LOCUS       NZ_NERK01000004         9234 bp    DNA     linear   CON 23-AUG-2024
DEFINITION  Bacillus halotolerans strain RSCu-8D Scaffold_4, whole genome
            shotgun sequence.
ACCESSION   NZ_NERK01000004
VERSION     NZ_NERK01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus halotolerans
  ORGANISM  Bacillus halotolerans
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 9234)
  AUTHORS   Gajera,H.P., Hirpara,D.G., Katakpara,Z.A., Kothari,V. and
            Golakiya,B.A.
  TITLE     Genome sequence of Bacillus halotolerans RSCu-8D
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9234)
  AUTHORS   Gajera,H.P., Hirpara,D.G., Katakpara,Z.A., Kothari,V. and
            Golakiya,B.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-APR-2017) Department of Biotechnology, Junagadh
            Agricultural University, Motibag, Junagadh, Gujarat 362 001, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-OCT-2016
            Assembly Method                   :: SPAdes v. 8.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 84.0x
            Sequencing Technology             :: IonTorrent
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002592025.1-RS_2024_08_23
            Annotation Date                   :: 08/23/2024 17:34:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,284
            CDSs (total)                      :: 4,199
            Genes (coding)                    :: 4,047
            CDSs (with protein)               :: 4,047
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 4, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 4, 2 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 101 of 152
            Pseudo Genes (incomplete)         :: 85 of 152
            Pseudo Genes (internal stop)      :: 26 of 152
            Pseudo Genes (multiple problems)  :: 53 of 152
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:21:27
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9234
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NERK01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..9234
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:>9234](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..>9234)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..5585)
                     /gene="fenA"
                     /locus_tag="B9T64_RS00320"
                     /old_locus_tag="B9T64_00320"
                     /pseudo=""
     CDS             complement(<1..5585)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-30). E-value: 1.8e-18.
                     Score: 57.5. Matches aSDomain:
                     nrpspksdomains_B9T64_RS00320_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (7-304). E-value:
                     4.6e-116. Score: 379.1. Matches aSDomain:
                     nrpspksdomains_B9T64_RS00320_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (468-864). E-value:
                     1.2e-106. Score: 348.7. Matches aSDomain:
                     nrpspksdomains_B9T64_RS00320_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (970-1039). E-value: 1.1e-23. Score:
                     75.2. Matches aSDomain: nrpspksdomains_B9T64_RS00320_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1054-1332). E-value:
                     4.7e-103. Score: 336.2. Matches aSDomain:
                     nrpspksdomains_B9T64_RS00320_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (1499-1861). E-value:
                     6.4e-98. Score: 319.9. Matches aSDomain:
                     nrpspksdomains_B9T64_RS00320_AMP-binding.2"
                     /codon_start=1
                     /gene="fenA"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389713.2"
                     /locus_tag="B9T64_RS00320"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="B9T64_00320"
                     /product="fengycin non-ribosomal peptide synthetase FenA"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 4.5e-115, bitscore:
                     371.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.2e-16, bitscore:
                     48.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 6.5e-88, bitscore:
                     281.7, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKANSIQDIYPLSYMQEGMLFHSLLQKDSRSYVEQASFTIEGEV
                     NLQFFQESINALVARHDIFRTIFISQNVSSPQQVVLRERTVSVLEEDIAHLDEAGQSAF
                     VERIKEKDRAKGFHLQKDMLMRITLIRTGMRQYTCIWTFHHIMMDGWCLGIVLKEFLQI
                     YASKIKGAPLSLEPVQPYGTYINWLMEQDKEKAVSYWDNYLSGIEQQTLLPKQKKTKST
                     SRQEQITFSFSKEDSSRLSELAVREEVTLSTIFHTLWGILLQKYNNSDDAVFGSVISGR
                     PSDIEGIEQMVGLFINTIPIRLHGSKTPFLQLVKDMQKDRLAAEAYSYHPLYEIQSRSA
                     VKQGLIDHILVFENYPVQKEIEMLNSQVPDSDLFQIHNFTVADETNYSFYLMVAPAEEI
                     HIKMSYDAALHDRSFVLSVKEHLLNTVSQILDHPHLSPEDIDITTDTEKLQLIGEIKDH
                     KPVYETIHAMFEKQAEKTPDARAVIDQAHSLTYKELNESANRLARHLRKKGVMRQEPVA
                     IMMERSTEFVTGILGILKAGGAIVPIDPHYPSDRINYILNDCGCSHVVSQAHFAPLLET
                     GLNVIYMEDIQIEDGSCLHSVNSADDLLYMIYTSGTTGKPKGVQFEHRNMANLLQFEFT
                     RSGIDFENDVLQFATPAFDVCYQEIFSTLLGGGTLHIVPEAIKRDVPQLFAFIKEHQTT
                     IVFLPTAFIKMIFSEQEFANAFPHDVKHLITAGEQLIVSSLLQDVLRQRSMYLHNHYGP
                     SETHVVSAYTIHPGNQIPEFPPIGKPIDCTDLYILNRQKQLQPRGVSGELYISGSSVAR
                     GYINNDKLTREKFLPDPFRSGAVMYRTGDVARIREDGSFEYIGRADDQVKIRGYRIEPQ
                     EIEIALMNHPEVKEAAILIRQNQGEEHELCAYYCSMHRLDSSALRQYLANKLPEYMIPA
                     KWVWVDSIPLTPNGKVDQSALPKPEASISSNTYAAPRNLLEVKLSQLFEDVLKEAPIGI
                     HDNFFDRGGHSLKATVLVSRIAKEFHVQVPLKDVFAHPTVEGLALIIRHAEENPFASVA
                     QAKEQPTYPVSSAQKRMYVLQHLDNGGVGYNMPAVLELEGKLDREKLAAVFKELISRHE
                     PLRTSFVPGEDAEPVQRIHSEVPFTLSEEASAAGFVRPFDLSQAPLFRAGLVKLANEKH
                     LLLVDMHHIISDGVSVQLLIREFTERYANRTLKPLRIQYKDYAVWQQEFQKGDTYKKQE
                     TYWLRQFSGELPVLELPTDKRRPAERNFTGGKVLFQMNEDITSRLKELARKNESTLYMT
                     LLALYSAFLSRLSGQEDIVIGSPIAGRPHADLEAVLGMFVNTLALRTHPAGNKTFTDFL
                     KEVKQTALEAYEHQDYPFEELVDQLGVQREMSRNPLFDTTLVLQNMEKQTLKMNDLLLR
                     WNDLQHPISKFDISLYVTEHEAELYCQFEYSTALFEEDTVQRWAGLFTTLVENTVSSPD
                     MELDDIPILTLEEERSFIDSCQPLEETGYRLKQTLHHALEQQAAKTPDQPAVIVEDEEI
                     TYKELNEQANRVAWELIDRGVKAETTVAIMGRRSPEMLIGIYAILKAGGAYLPIDPDYP
                     EERIRFLLKDSDSDILLIQSDNLDIPAFDGEIVHLSQLNSGLKRRLSNPNVEVYPDSLA
                     YMIYTSGSTGRPKGVQVEHQSAVNFLNSLQFRYPLNQSDVILHKTSYSFDASIWELFWW
                     PYGGASVYLLPQGGEKEPDMILKVIEEQQITAMHFVPSMLHAFLEYLKNGPVPIKTNRL
                     KRVFSGGEQLGAHLVSRFCELLPDVTLTNSYGPTEATVEAAFFDCPLDEKLDRIPIGKP
                     IHHVRLYILNQKQKMLPAGCIGELYIAGAGVARGYLNRPELTEERF"
     source          1..9234
                     /collected_by="Darshana G. Hirpara"
                     /collection_date="2015-10-10"
                     /db_xref="taxon:260554"
                     /geo_loc_name="India: Gujarat, Jasdan"
                     /host="Cuminum cyminum"
                     /lat_lon="21.52 N 70.45 E"
                     /mol_type="genomic DNA"
                     /organism="Bacillus halotolerans"
                     /strain="RSCu-8D"
                     /submitter_seqid="Scaffold_4"
     region          1..9234
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9234
                     /SMILES="NC([*])C(=O)N1C(CCC1)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(3..92)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0019"
                     /evalue="1.70E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="1861"
                     /protein_start="1831"
                     /score="58.6"
                     /tool="antismash"
                     /translation="PAGCIGELYIAGAGVARGYLNRPELTEERF"
     aSDomain        complement(3..1088)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS00320_AMP-binding.2"
                     /evalue="6.40E-98"
                     /label="B9T64_RS00320_AMP-binding.2"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="1861"
                     /protein_start="1499"
                     /score="319.9"
                     /specificity="substrate consensus: Glu"
                     /tool="antismash"
                     /translation="LEQQAAKTPDQPAVIVEDEEITYKELNEQANRVAWELIDRGVKAE
                     TTVAIMGRRSPEMLIGIYAILKAGGAYLPIDPDYPEERIRFLLKDSDSDILLIQSDNLD
                     IPAFDGEIVHLSQLNSGLKRRLSNPNVEVYPDSLAYMIYTSGSTGRPKGVQVEHQSAVN
                     FLNSLQFRYPLNQSDVILHKTSYSFDASIWELFWWPYGGASVYLLPQGGEKEPDMILKV
                     IEEQQITAMHFVPSMLHAFLEYLKNGPVPIKTNRLKRVFSGGEQLGAHLVSRFCELLPD
                     VTLTNSYGPTEATVEAAFFDCPLDEKLDRIPIGKPIHHVRLYILNQKQKMLPAGCIGEL
                     YIAGAGVARGYLNRPELTEERF"
     aSModule        complement(3..2423)
                     /domains="nrpspksdomains_B9T64_RS00320_Condensation_LCL.1"
                     /domains="nrpspksdomains_B9T64_RS00320_AMP-binding.2"
                     /incomplete
                     /locus_tags="B9T64_RS00320"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(201..230)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0018"
                     /evalue="3.90E-02"
                     /label="NRPS-A_a5"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="1795"
                     /protein_start="1785"
                     /score="7.1"
                     /tool="antismash"
                     /translation="SYGPTEATVE"
     CDS_motif       complement(615..674)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0017"
                     /evalue="3.80E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="1657"
                     /protein_start="1637"
                     /score="31.6"
                     /tool="antismash"
                     /translation="AYMIYTSGSTGRPKGVQVEH"
     CDS_motif       complement(861..899)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0016"
                     /evalue="4.80E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="1575"
                     /protein_start="1562"
                     /score="16.6"
                     /tool="antismash"
                     /translation="AILKAGGAYLPID"
     CDS_motif       complement(1428..1547)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0015"
                     /evalue="1.00E-20"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="1386"
                     /protein_start="1346"
                     /score="66.2"
                     /tool="antismash"
                     /translation="KQTALEAYEHQDYPFEELVDQLGVQREMSRNPLFDTTLVL"
     aSDomain        complement(1590..2423)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS00320_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="4.70E-103"
                     /label="B9T64_RS00320_Condensation_LCL.1"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="1332"
                     /protein_start="1054"
                     /score="336.2"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="TYPVSSAQKRMYVLQHLDNGGVGYNMPAVLELEGKLDREKLAAVF
                     KELISRHEPLRTSFVPGEDAEPVQRIHSEVPFTLSEEASAAGFVRPFDLSQAPLFRAGL
                     VKLANEKHLLLVDMHHIISDGVSVQLLIREFTERYANRTLKPLRIQYKDYAVWQQEFQK
                     GDTYKKQETYWLRQFSGELPVLELPTDKRRPAERNFTGGKVLFQMNEDITSRLKELARK
                     NESTLYMTLLALYSAFLSRLSGQEDIVIGSPIAGRPHADLEAVLGMFVNTLALRTH"
     CDS_motif       complement(1596..1685)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0014"
                     /evalue="2.50E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="1330"
                     /protein_start="1300"
                     /score="52.0"
                     /tool="antismash"
                     /translation="DIVIGSPIAGRPHADLEAVLGMFVNTLALR"
     CDS_motif       complement(1944..1976)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0013"
                     /evalue="2.60E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="1214"
                     /protein_start="1203"
                     /score="13.7"
                     /tool="antismash"
                     /translation="QYKDYAVWQQE"
     CDS_motif       complement(2007..2072)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0012"
                     /evalue="9.80E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="1193"
                     /protein_start="1171"
                     /score="27.7"
                     /tool="antismash"
                     /translation="MHHIISDGVSVQLLIREFTERY"
     CDS_motif       complement(2244..2354)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0011"
                     /evalue="2.70E-14"
                     /label="C2_LCL_024-062"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="1114"
                     /protein_start="1077"
                     /score="45.5"
                     /tool="antismash"
                     /translation="YNMPAVLELEGKLDREKLAAVFKELISRHEPLRTSFV"
     aSDomain        complement(2469..2675)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS00320_PCP.1"
                     /evalue="1.10E-23"
                     /label="B9T64_RS00320_PCP.1"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="1039"
                     /protein_start="970"
                     /score="75.2"
                     /tool="antismash"
                     /translation="EVKLSQLFEDVLKEAPIGIHDNFFDRGGHSLKATVLVSRIAKEFH
                     VQVPLKDVFAHPTVEGLALIIRHA"
     aSModule        complement(2469..5564)
                     /complete
                     /domains="nrpspksdomains_B9T64_RS00320_Condensation_DCL.1"
                     /domains="nrpspksdomains_B9T64_RS00320_AMP-binding.1"
                     /domains="nrpspksdomains_B9T64_RS00320_PCP.1"
                     /locus_tags="B9T64_RS00320"
                     /monomer_pairings="Pro -> Pro"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(2964..3029)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0010"
                     /evalue="2.30E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="874"
                     /protein_start="852"
                     /score="38.7"
                     /tool="antismash"
                     /translation="YIGRADDQVKIRGYRIEPQEIE"
     aSDomain        complement(2994..4181)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS00320_AMP-binding.1"
                     /evalue="1.20E-106"
                     /label="B9T64_RS00320_AMP-binding.1"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="864"
                     /protein_start="468"
                     /score="348.7"
                     /specificity="substrate consensus: Pro"
                     /tool="antismash"
                     /translation="FEKQAEKTPDARAVIDQAHSLTYKELNESANRLARHLRKKGVMRQ
                     EPVAIMMERSTEFVTGILGILKAGGAIVPIDPHYPSDRINYILNDCGCSHVVSQAHFAP
                     LLETGLNVIYMEDIQIEDGSCLHSVNSADDLLYMIYTSGTTGKPKGVQFEHRNMANLLQ
                     FEFTRSGIDFENDVLQFATPAFDVCYQEIFSTLLGGGTLHIVPEAIKRDVPQLFAFIKE
                     HQTTIVFLPTAFIKMIFSEQEFANAFPHDVKHLITAGEQLIVSSLLQDVLRQRSMYLHN
                     HYGPSETHVVSAYTIHPGNQIPEFPPIGKPIDCTDLYILNRQKQLQPRGVSGELYISGS
                     SVARGYINNDKLTREKFLPDPFRSGAVMYRTGDVARIREDGSFEYIGRADDQVKIR"
     CDS_motif       complement(3111..3200)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0009"
                     /evalue="2.90E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="825"
                     /protein_start="795"
                     /score="42.0"
                     /tool="antismash"
                     /translation="PRGVSGELYISGSSVARGYINNDKLTREKF"
     CDS_motif       complement(3309..3335)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0008"
                     /evalue="4.10E-01"
                     /label="NRPS-A_a5"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="759"
                     /protein_start="750"
                     /score="4.2"
                     /tool="antismash"
                     /translation="YGPSETHVV"
     CDS_motif       complement(3717..3773)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0007"
                     /evalue="1.30E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="623"
                     /protein_start="604"
                     /score="27.0"
                     /tool="antismash"
                     /translation="YMIYTSGTTGKPKGVQFEH"
     CDS_motif       complement(3954..3995)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0006"
                     /evalue="2.70E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="544"
                     /protein_start="530"
                     /score="14.3"
                     /tool="antismash"
                     /translation="LGILKAGGAIVPID"
     CDS_motif       complement(4518..4634)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0005"
                     /evalue="1.80E-20"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="356"
                     /protein_start="317"
                     /score="65.4"
                     /tool="antismash"
                     /translation="QKDRLAAEAYSYHPLYEIQSRSAVKQGLIDHILVFENYP"
     aSDomain        complement(4674..5564)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS00320_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="4.60E-116"
                     /label="B9T64_RS00320_Condensation_DCL.1"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="304"
                     /protein_start="7"
                     /score="379.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="QDIYPLSYMQEGMLFHSLLQKDSRSYVEQASFTIEGEVNLQFFQE
                     SINALVARHDIFRTIFISQNVSSPQQVVLRERTVSVLEEDIAHLDEAGQSAFVERIKEK
                     DRAKGFHLQKDMLMRITLIRTGMRQYTCIWTFHHIMMDGWCLGIVLKEFLQIYASKIKG
                     APLSLEPVQPYGTYINWLMEQDKEKAVSYWDNYLSGIEQQTLLPKQKKTKSTSRQEQIT
                     FSFSKEDSSRLSELAVREEVTLSTIFHTLWGILLQKYNNSDDAVFGSVISGRPSDIEGI
                     EQMVGLFINTIPIRLH"
     CDS_motif       complement(4680..4775)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0004"
                     /evalue="2.50E-19"
                     /label="C5_DCL_263-294"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="302"
                     /protein_start="270"
                     /score="61.7"
                     /tool="antismash"
                     /translation="DAVFGSVISGRPSDIEGIEQMVGLFINTIPIR"
     CDS_motif       complement(5094..5159)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0003"
                     /evalue="2.80E-12"
                     /label="C3_DCL_135-156"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="164"
                     /protein_start="142"
                     /score="39.0"
                     /tool="antismash"
                     /translation="FHHIMMDGWCLGIVLKEFLQIY"
     CDS_motif       complement(5379..5492)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0002"
                     /evalue="6.20E-16"
                     /label="C2_DCL_024-062"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="69"
                     /protein_start="31"
                     /score="50.7"
                     /tool="antismash"
                     /translation="SYVEQASFTIEGEVNLQFFQESINALVARHDIFRTIFI"
     aSDomain        complement(5496..5585)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS00320_NRPS-COM_Nterm.1"
                     /evalue="1.80E-18"
                     /label="B9T64_RS00320_NRPS-COM_Nterm.1"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="30"
                     /protein_start="0"
                     /score="57.5"
                     /tool="antismash"
                     /translation="MTKANSIQDIYPLSYMQEGMLFHSLLQKDS"
     CDS_motif       complement(5514..5555)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00320_0001"
                     /evalue="8.60E-05"
                     /label="C1_DCL_004-017"
                     /locus_tag="B9T64_RS00320"
                     /protein_end="24"
                     /protein_start="10"
                     /score="14.5"
                     /tool="antismash"
                     /translation="YPLSYMQEGMLFHS"
     gene            complement(5611..>9234)
                     /gene="fenE"
                     /locus_tag="B9T64_RS00325"
                     /old_locus_tag="B9T64_00325"
                     /pseudo=""
     CDS             complement(5611..>9234)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: AMP-binding (160-555). E-value:
                     2.5e-113. Score: 370.7. Matches aSDomain:
                     nrpspksdomains_B9T64_RS00325_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (660-728). E-value: 2.5e-24. Score:
                     77.3. Matches aSDomain: nrpspksdomains_B9T64_RS00325_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (738-1041). E-value:
                     1.7e-167. Score: 550.5. Matches aSDomain:
                     nrpspksdomains_B9T64_RS00325_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (1047-1200). E-value: 5.4e-49.
                     Score: 157.9. Matches aSDomain:
                     nrpspksdomains_B9T64_RS00325_TIGR01720.1"
                     /codon_start=1
                     /gene="fenE"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389714.1"
                     /locus_tag="B9T64_RS00325"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="B9T64_00325"
                     /product="fengycin non-ribosomal peptide synthetase FenE"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 7.5e-123, bitscore:
                     397.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.4e-16, bitscore:
                     46.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2.7e-78, bitscore:
                     250.1, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="YLPQEVRETALEAYEHQDYPFEELVDKLGMTRDMSRNPLFDVMFV
                     LQNMDQESMQLKDLCLRPATNVEHQVSKFDLTLYAYEEANGSMMFQMEYSTDLYQKNTI
                     EMWLHYLMNMLRAIIQDSEAALGTIHVLDENETHFLIHELNCTKREYPRHETISRLFEL
                     QAAQTPDAIAVAGDEQTLTYKELNIRANRIAAVLRRKGVGPETVVALLTTRTPEIAAGM
                     LGILKAGGAYLPIAKDLPADRISYMLSDSGAKILLQSEKANNQRLDLELKCEKIVIEEI
                     QGQGETKNFESPAGPHSLAYIIYTSGSTGKPKGVMIEQRSVIRLVKNSNYIEFTPEDRL
                     LLTSSLGFDVVTFEILGPLLNGAALHLTDKETFLDSHQLKRYIEQNGITTMWLTASLFN
                     HLTEQNEKIFSRLINLIIGGEALSASHVNRIKQACPELSIWNGYGPTENTTFSTCFHIE
                     KLYEHSIPIGRPIGNSTAFILNKWGMLQPIGAVGELCVGGDGVARGYLGRPDLTKEKFV
                     PNPFAPGEQMYRTGDLARWLPDGTIEYVGRVDDQVKIRGYRVELGEIESALRHIDGVKE
                     AAVLARTGQLGTKELYAYISVKEGTDAEQVRTHLSQMLPGYMMPAYVIEMDALPLTANG
                     KLNRKALPEPDITSKQTYVPPRNDLEEQLAIIWQEVLGTQRIGIEDSFFELGGDSIKAL
                     QVSARLGRYGWSLHASDLFRHPKIKDLSAVIRKTERVIDQGSVQGAVPWTPIQHWFLSQ
                     DIKERHHFNQSVMLFSPDCLSENALRASLKKLAEHHDALRMIYREDSGQQMQINQDIHE
                     SKLYSLRISDLSDSGMDWETSIKEEVANLQQSINLQQGPLLHAAWFKTLSGDYLFLTIH
                     HLVVDGVSWRILLEDLSAAYHQAASGQAIQLPPKTDSYQEYARRVQDYAQSSKLIREET
                     YWRSVEEEKAAELPYEMPYMENMNSSERETLRFSLTEADTAVLLQKVNHAYGTDTQDIL
                     LTAASLAICDWTGGSKLRMAMEGHGREHILPDLDISRTVGWFTSIYPVLIDFENQADEL
                     GPAVKTVKDTLGRIPNKGVGYGMLKYLTPPEHKSMVFSKTPEIGFNYLGQFNDIESQDR
                     FRPSGLGSGKDITPTWKREQLIEMSAMAAENQLHFQLSYPPARFHRGAMERLITMIECY
                     LQDIMKHCAEKQNTEKTLSDFSSQSLTAEDLDSISSLVEDL"
     aSDomain        complement(5632..6090)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS00325_TIGR01720.1"
                     /evalue="5.40E-49"
                     /label="B9T64_RS00325_TIGR01720.1"
                     /locus_tag="B9T64_RS00325"
                     /protein_end="1200"
                     /protein_start="1047"
                     /score="157.9"
                     /tool="antismash"
                     /translation="LGPAVKTVKDTLGRIPNKGVGYGMLKYLTPPEHKSMVFSKTPEIG
                     FNYLGQFNDIESQDRFRPSGLGSGKDITPTWKREQLIEMSAMAAENQLHFQLSYPPARF
                     HRGAMERLITMIECYLQDIMKHCAEKQNTEKTLSDFSSQSLTAEDLDSI"
     aSModule        complement(5632..8751)
                     /complete
                     /domains="nrpspksdomains_B9T64_RS00325_AMP-binding.1"
                     /domains="nrpspksdomains_B9T64_RS00325_PCP.1"
                     /domains="nrpspksdomains_B9T64_RS00325_Epimerization.1"
                     /domains="nrpspksdomains_B9T64_RS00325_TIGR01720.1"
                     /locus_tags="B9T64_RS00325"
                     /monomer_pairings="X -> D-X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(5938..5967)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00325_0011"
                     /evalue="6.10E-03"
                     /label="NRPS-E7"
                     /locus_tag="B9T64_RS00325"
                     /protein_end="1098"
                     /protein_start="1088"
                     /score="9.2"
                     /tool="antismash"
                     /translation="PEIGFNYLGQ"
     CDS_motif       complement(6007..6069)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00325_0010"
                     /evalue="1.20E-09"
                     /label="NRPS-E6"
                     /locus_tag="B9T64_RS00325"
                     /protein_end="1075"
                     /protein_start="1054"
                     /score="30.4"
                     /tool="antismash"
                     /translation="VKDTLGRIPNKGVGYGMLKYL"
     aSDomain        complement(6109..7017)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS00325_Epimerization.1"
                     /evalue="1.70E-167"
                     /label="B9T64_RS00325_Epimerization.1"
                     /locus_tag="B9T64_RS00325"
                     /protein_end="1041"
                     /protein_start="738"
                     /score="550.5"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="GAVPWTPIQHWFLSQDIKERHHFNQSVMLFSPDCLSENALRASLK
                     KLAEHHDALRMIYREDSGQQMQINQDIHESKLYSLRISDLSDSGMDWETSIKEEVANLQ
                     QSINLQQGPLLHAAWFKTLSGDYLFLTIHHLVVDGVSWRILLEDLSAAYHQAASGQAIQ
                     LPPKTDSYQEYARRVQDYAQSSKLIREETYWRSVEEEKAAELPYEMPYMENMNSSERET
                     LRFSLTEADTAVLLQKVNHAYGTDTQDILLTAASLAICDWTGGSKLRMAMEGHGREHIL
                     PDLDISRTVGWFTSIYPVLIDF"
     CDS_motif       complement(6118..6165)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00325_0009"
                     /evalue="2.60E-09"
                     /label="NRPS-E5"
                     /locus_tag="B9T64_RS00325"
                     /protein_end="1038"
                     /protein_start="1022"
                     /score="28.9"
                     /tool="antismash"
                     /translation="DISRTVGWFTSIYPVL"
     CDS_motif       complement(6571..6636)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00325_0008"
                     /evalue="7.70E-13"
                     /label="NRPS-E2"
                     /locus_tag="B9T64_RS00325"
                     /protein_end="887"
                     /protein_start="865"
                     /score="40.4"
                     /tool="antismash"
                     /translation="LFLTIHHLVVDGVSWRILLEDL"
     CDS_motif       complement(6844..6909)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00325_0007"
                     /evalue="6.90E-04"
                     /label="C2_LCL_024-062"
                     /locus_tag="B9T64_RS00325"
                     /protein_end="796"
                     /protein_start="774"
                     /score="12.1"
                     /tool="antismash"
                     /translation="ENALRASLKKLAEHHDALRMIY"
     CDS_motif       complement(6979..7005)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00325_0006"
                     /evalue="2.10E-03"
                     /label="NRPS-E1"
                     /locus_tag="B9T64_RS00325"
                     /protein_end="751"
                     /protein_start="742"
                     /score="11.2"
                     /tool="antismash"
                     /translation="WTPIQHWFL"
     aSDomain        complement(7048..7251)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS00325_PCP.1"
                     /evalue="2.50E-24"
                     /label="B9T64_RS00325_PCP.1"
                     /locus_tag="B9T64_RS00325"
                     /protein_end="728"
                     /protein_start="660"
                     /score="77.3"
                     /tool="antismash"
                     /translation="EEQLAIIWQEVLGTQRIGIEDSFFELGGDSIKALQVSARLGRYGW
                     SLHASDLFRHPKIKDLSAVIRKT"
     CDS_motif       complement(7537..7602)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00325_0005"
                     /evalue="1.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="B9T64_RS00325"
                     /protein_end="565"
                     /protein_start="543"
                     /score="39.5"
                     /tool="antismash"
                     /translation="YVGRVDDQVKIRGYRVELGEIE"
     aSDomain        complement(7567..8751)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9T64_RS00325_AMP-binding.1"
                     /evalue="2.50E-113"
                     /label="B9T64_RS00325_AMP-binding.1"
                     /locus_tag="B9T64_RS00325"
                     /protein_end="555"
                     /protein_start="160"
                     /score="370.7"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FELQAAQTPDAIAVAGDEQTLTYKELNIRANRIAAVLRRKGVGPE
                     TVVALLTTRTPEIAAGMLGILKAGGAYLPIAKDLPADRISYMLSDSGAKILLQSEKANN
                     QRLDLELKCEKIVIEEIQGQGETKNFESPAGPHSLAYIIYTSGSTGKPKGVMIEQRSVI
                     RLVKNSNYIEFTPEDRLLLTSSLGFDVVTFEILGPLLNGAALHLTDKETFLDSHQLKRY
                     IEQNGITTMWLTASLFNHLTEQNEKIFSRLINLIIGGEALSASHVNRIKQACPELSIWN
                     GYGPTENTTFSTCFHIEKLYEHSIPIGRPIGNSTAFILNKWGMLQPIGAVGELCVGGDG
                     VARGYLGRPDLTKEKFVPNPFAPGEQMYRTGDLARWLPDGTIEYVGRVDDQVKIR"
     CDS_motif       complement(7684..7773)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00325_0004"
                     /evalue="3.70E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="B9T64_RS00325"
                     /protein_end="516"
                     /protein_start="486"
                     /score="57.5"
                     /tool="antismash"
                     /translation="PIGAVGELCVGGDGVARGYLGRPDLTKEKF"
     CDS_motif       complement(8278..8334)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00325_0003"
                     /evalue="1.00E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="B9T64_RS00325"
                     /protein_end="318"
                     /protein_start="299"
                     /score="33.4"
                     /tool="antismash"
                     /translation="AYIIYTSGSTGKPKGVMIE"
     CDS_motif       complement(8527..8565)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00325_0002"
                     /evalue="1.60E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="B9T64_RS00325"
                     /protein_end="235"
                     /protein_start="222"
                     /score="15.0"
                     /tool="antismash"
                     /translation="LGILKAGGAYLPI"
     CDS_motif       complement(9094..9213)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B9T64_RS00325_0001"
                     /evalue="6.20E-23"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="B9T64_RS00325"
                     /protein_end="46"
                     /protein_start="6"
                     /score="73.3"
                     /tool="antismash"
                     /translation="RETALEAYEHQDYPFEELVDKLGMTRDMSRNPLFDVMFVL"
ORIGIN
        1 agaaaacgtt cttctgttaa ttccggacga ttcaagtagc ctctggcgac gccggctccg
       61 gcgatataca attctccgat acatccagca ggcagcatct tctgcttctg gttcagaata
      121 tacaatctga catgatgaat aggtttgcca atgggaatgc ggtcaagttt ttcatccagc
      181 gggcaatcaa agaatgccgc ttctacagta gcttcggtag gcccatagga attggttaat
      241 gtaacatctg gcagcaattc acagaaccga gagacaagat gtgcccccag ctgttcgcct
      301 ccagaaaaca ccctcttcag cctgttcgtt ttgatcggta cagggccatt ttttagatat
      361 tctaaaaacg catgaagcat agaaggcaca aaatgcatcg ctgtaatctg ctgttcttct
      421 atgaccttca atatcatgtc aggttctttt tcgcctcctt gaggaagcag ataaacagag
      481 gcgccaccgt acggccacca aaacagctcc catatcgaag catcgaagga atacgacgtt
      541 ttatgcaaaa tgacgtccga ttggttcaaa ggatagcgga actgaaggga atttaaaaaa
      601 ttcactgcag attgatgctc aacctgtaca cccttaggtc ttccagtaga gccggatgta
      661 taaatcatat aggctaaaga atccggatag acttcaacat ttggattgga cagacgacgt
      721 ttcagcccgc tgtttaattg actgagatga acgatttctc catcaaaagc cggaatgtct
      781 agattgtcag attgaataag gagaatgtca gaatcgctgt ccttcagcaa aaagcggatc
      841 cgttcctctg gataatccgg gtcaatagga agataagcac cgcctgcttt tagaatcgca
      901 taaatcccga tcagcatttc aggggaacgc cttcccataa tggcaacagt cgtttcggct
      961 tttactcccc ggtcgatcag ctcccaggct acacggttag cctgttcgtt caattccttg
     1021 taggttatct cttcgtcttc aacgatcacg gccggttggt caggtgtttt tgccgcttgc
     1081 tgttcgagtg catggtgcag ggtctgtttc aaccgatagc ctgtctcttc aagcggctgg
     1141 catgagtcaa tgaaagacct ttcttcttct aaagtcagga tcggaatatc gtccaattcc
     1201 atgtcaggac tggaaactgt gttttccaca agtgtcgtaa aaaggccggc ccacctttga
     1261 acggtatctt cttcaaacaa agctgtactg tattcaaact gacagtacag ctcagcttca
     1321 tgctctgtga catacaggga aatatcgaac ttagatatcg gatgctgcaa atcgttccat
     1381 ctaagcagca aatcattcat tttcagcgtt tgcttttcca tattttgcag cacaagtgtt
     1441 gtatcaaaca gcggattccg gctcatttcc cgctgcacac cgagttgatc aaccagctct
     1501 tcaaacggat aatcctgatg ctcataggct tccagtgcag tctgcttgac ttccttcaga
     1561 aaatccgtaa atgtcttatt gccggctggg tgcgtccgga gcgctaatgt attgacgaac
     1621 ataccaagga ctgcttcaag atcggcatgc gggcgtccgg caataggtga accgatcacg
     1681 atgtcttcct gaccgcttaa ccgcgacaaa aaagctgaat acaatgcgag tagcgtcatg
     1741 tacagcgtgc tctcattttt gcgggctagc tccttcaggc gggacgtgat gtcttcattc
     1801 atttgaaata aaactttgcc gcctgtgaaa ttccgttctg ccggtcggcg cttgtctgtt
     1861 gggagctcga gcaccggcag ctcaccgcta aactgtctca gccagtaagt ctcctgcttt
     1921 ttgtatgtgt ctcccttttg aaattcctgc tgccaaacgg cgtaatcttt atattggatt
     1981 cgtaacggct taagcgtccg atttgcatag cgctcagtga attcgcggat taaaagctga
     2041 acggatacac cgtcggaaat gatatgatgc atgtccacca gcagcaaatg cttctcgttc
     2101 gccagtttca caaggccagc tctgaataac ggcgcttgac tgaggtcaaa tggacggaca
     2161 aatccagcag cagaggcttc ttcacttaac gtgaacggaa cctcactgtg aatacgctgt
     2221 acgggctcgg catcttctcc cggcacaaag gacgttcgaa gcggctcatg ccggctgatc
     2281 agttccttga atacagcagc cagtttttcg cgatcaagct tcccttccag ttctaaaact
     2341 gccggcatgt tgtagccgac tcctccatta tctagatgct ggagcacgta catccgcttt
     2401 tgagctgatg atactgggta cgtcggttgt tcttttgcct gtgcaaccga cgcaaaggga
     2461 ttttcctctg catgtcgaat aatgagagcc aatccttcca ctgtcggatg ggcaaatacg
     2521 tccttcagcg ggacttgaac atgaaattcc ttggcgattc gcgacacaag caccgttgct
     2581 tttaacgagt gtccgccgcg gtcaaaaaag ttgtcgtgaa tgccgatcgg ggcttccttc
     2641 agcacatctt caaacagctg cgaaagcttt acctctaaca ggttccgcgg agcagcgtat
     2701 gtattactgc tgattgaagc ttccggtttt ggaagagctg attgatcgac ttttccgttc
     2761 ggcgtcaaag gaatgctgtc aacccagacc catttggccg gaatcatata ttccgggagc
     2821 ttatttgcca gatattgtcg taaagccgag gaatccagcc tgtgcatact gcaataataa
     2881 gcgcataact catgttcttc accttgattt tgccggataa gaatagcagc ttctttgacc
     2941 tctggatgat tcattaacgc gatttctatt tcttgcggct cgattcgata acctctgatt
     3001 ttcacttgat cgtcggctcg accgatatat tcaaagctgc cgtcttcacg aattctcgct
     3061 acatctcccg ttcggtacat gaccgctccg gacctgaagg gatcgggtaa aaatttctct
     3121 cgcgtcagtt tatcgttatt gatataacct ctggcgacac ttgaaccgga aatatatagt
     3181 tctcctgata ccccccgagg ctgcagctgt ttttggcgat ttaaaatata cagatcagta
     3241 caatcaatcg gttttccgat cggaggaaac tccggaattt ggttacccgg atgtatggta
     3301 tatgccgaca ctacatgagt ttctgaaggt ccataatgat tgtgcaagta catgctgcgc
     3361 tggcgcaaaa catcttggag aagactggaa acgattaatt gctcaccggc tgtaatcagg
     3421 tgttttacat catgaggaaa cgcgttcgca aattcctgtt cgctgaaaat cattttgata
     3481 aaagccgttg ggagaaacac aatagtcgtc tgatgctctt ttataaatgc aaacagctga
     3541 ggtacgtctc tttttatagc ttctggcacg atatgaagtg tgccgcctcc cagcaatgtt
     3601 gaaaatattt cttgatagca gacgtcaaag gcaggagttg caaattgcaa aacatcattt
     3661 tcaaaatcaa tgccagagcg agtgaattcg aactgcagca agttagccat atttcgatgt
     3721 tcaaattgaa cgccctttgg ttttcctgtt gtgcctgatg tgtaaatcat atagagcagg
     3781 tcgtccgcac tattcactga atgtaagcag ctgccgtctt cgatttgaat atcttccata
     3841 tagataacat tcaggccagt ctcaagcaaa ggtgcaaaat gcgcttgtga aacgacatga
     3901 gagcagccgc agtcattcag aatataattt attctgtcag acggataatg gggatcgatt
     3961 ggaacaatgg cgcctccagc cttcaatata ccgagaatac ctgtaacgaa ctcagttgag
     4021 cgttccatca tgattgcgac aggttcctgc ctcataacgc cttttttccg caaatgccgt
     4081 gccaatctgt tggcggattc gttcaattct ttataggtca gagaatgcgc ttgatcaatc
     4141 acagcccgcg cgtcaggcgt cttttctgcc tgtttttcaa acatggcatg gatggtttcg
     4201 taaactggtt tgtgatcttt gatttcccca atcagctgca gcttttcagt gtctgttgtg
     4261 atatcgatgt cttcgggtga gagatgcgga tgatctaaga tttgggaaac agtgttcaaa
     4321 agatgctcct taacagaaag cacaaaggat cggtcgtgca gtgctgcatc atagctcatt
     4381 tttatatgga tttcttccgc tggcgccacc ataagataaa agctgtaatt ggtttcatcc
     4441 gctactgtaa agttatgaat ctgaaaaaga tctgaatcag gcacctggct gttcagcatt
     4501 tctatttctt tctgcaccgg atagttttcg aatacaagaa tgtgatcaat caaaccttgc
     4561 ttgactgctg aacgggactg tatctcgtat agaggatggt agctgtatgc ttctgcagca
     4621 aggcgatcct tttgcatgtc ttttacgagc tgtaaaaatg gcgtttttga tccatgcagc
     4681 cgtatgggaa tcgtgttgat gaaaagcccg accatttgtt caattccttc aatgtctgag
     4741 ggccttcctg aaatgacaga accgaatacc gcgtcatcac tattgttata tttttgaaga
     4801 agaattcccc atagggtatg gaaaattgta ctgagtgtga cttcttctct tacggcaagc
     4861 tctgacagcc tgctgctgtc ttcttttgaa aatgaaaatg taatttgctc ctgcctgctt
     4921 gtactctttg tttttttctg cttcgggagc aaggtttgct gctcaatgcc ggaaagataa
     4981 ttgtcccaat aggaaacagc tttctctttg tcttgctcca tcagccaatt gatataagtt
     5041 ccgtatggct gaacgggctc caatgataat ggagcccctt ttatttttga tgcataaatc
     5101 tgaaggaatt cttttagtac aatgccgagg caccagccgt ccatcatgat gtgatgaaag
     5161 gtccaaatac aagtatattg ccgcattccg gttcgaataa gtgtgatgcg catgagcata
     5221 tctttttgca gatgaaaccc tttggcgcgg tccttttctt ttattcgttc gacaaacgca
     5281 gattggcccg cttcatctaa atgagcaatg tcctcctcca gtacactgac agtccgttcc
     5341 ctcaggacaa cctgctgagg tgaagagaca ttttggctga tgaaaatcgt tctgaaaatg
     5401 tcatgtcttg ctactaaagc attgatgctc tcttggaaaa actgcaaatt gacttctccc
     5461 tctattgtaa aagaggcttg ttcaacatat gaccgtgaat ctttttgcag gagggaatga
     5521 aagagcattc cttcctgcat ataagacagc ggataaatat cctgaattga attcgctttt
     5581 gtcatgttca tatctccctg tcacgatttt ctacaagtct tccaccaagc tggatatact
     5641 gtccaaatcc tctgccgtta atgattggct gctgaaatcg ctcagcgtct tttctgtgtt
     5701 ctgtttttca gcacagtgtt tcataatatc ctgcaaatag cattcaatca tggtgatgag
     5761 ccgctccatt gcacccctgt gaaaccgtgc cggaggatag ctgagctgaa aatgaagctg
     5821 attttctgct gccatcgcac tcatctcaat aagctgctcc cgcttccaag taggagtgat
     5881 gtccttaccg ctcccaagac cggatgggcg gaaacggtct tgactttcta tgtcgttgaa
     5941 ctgccctaaa tagttaaagc cgatttcagg tgtttttgaa aacaccatgc ttttatgttc
     6001 gggaggtgta agatatttca acatgccgta ccctacccct ttgttcggta tccggcccaa
     6061 tgtatctttc acggttttaa ctgcgggtcc aagctcatcc gcttgatttt caaagtcaat
     6121 aaggacgggg taaatggatg taaaccagcc aaccgtccgg ctgatgtcta aatccggcag
     6181 aatgtgttct cttccgtgtc cctccattgc catacggagc ttgctgcccc ctgtccagtc
     6241 acaaatcgct aaggaagccg ccgtcaatag aatatcttgt gtatctgtgc cataagcgtg
     6301 gtttaccttt tgcagcaaga ccgcggtgtc tgcttcagtg agtgaaaagc gcaatgtttc
     6361 cctttcactg ctgttcatat tctccatata aggcatttca tatggcagtt ccgctgcttt
     6421 ttcctcttca accgaccgcc agtacgtttc ctcgcgaatc agcttgctgc tttgggcata
     6481 atcttgaact cgccgtgcgt attcctgata ggaatctgtt ttaggcggaa gctgtatagc
     6541 ctgtcctgaa gctgcttgat gatatgccgc tgacaggtcc tccagtaaaa ttctccatga
     6601 taccccatca acgaccaaat gatgaatcgt gagaaataaa taatctccgg acagcgtttt
     6661 aaaccaagcg gcatgcagta atggtccttg ttggagattg atactttgct gaagatttgc
     6721 cacttcttct ttaatgctcg tttcccaatc cattcccgaa tctgataaat cagaaatcct
     6781 taagctgtac agtttggatt catgaatatc ttggttgatt tgcatctgtt gtccgctatc
     6841 ttcccgataa atcatccgca gggcatcatg atgctctgcc agttttttta atgatgcgcg
     6901 cagtgcattt tccgacaaac aatcagggct aaacagcatg acagactggt taaaatggtg
     6961 tctttctttt atatcctgag agaggaacca atgctgaata ggtgtccatg ggacagctcc
     7021 ctgaacagat ccttggtcta ttactctttc tgtcttccgg atgactgctg ataaatcttt
     7081 tattttggga tggcggaata aatcgcttgc atgcaagctc catccataac gtccgaggcg
     7141 cgcggatact tgaagagcct taattgaatc tccgcctagt tcaaaaaacg aatcttctat
     7201 tccgatcctc tgtgtcccga gaacctcctg ccatatgatc gcaagctgtt cttcaaggtc
     7261 atttcgaggc gggacgtacg tttgtttaga tgtgatatct ggttcaggca gtgcttttcg
     7321 gttcaatttt ccgttggcag tgagcgggag agcatccatt tctatcacat aggccggcat
     7381 catatagcct ggcagcattt gagaaaggtg agtacgcact tgttcagcat cagtaccctc
     7441 ttttacgctt atataggcgt aaagctcctt ggtgcctaat tgtcctgttc gcgccaatac
     7501 ggctgcttct ttgacaccgt ctatatgacg aagcgctgat tcaatctcac cgagctcaac
     7561 ccggtaccct cttattttta cctgatcatc gacacggcct acatattcaa ttgtgccgtc
     7621 cggtagccag cgtgccagat caccggtacg gtacatttgt tcgccgggcg caaatggatt
     7681 tggcacaaat ttctctttcg tcaggtctgg tcgaccaaga tagcctcttg ccacaccatc
     7741 tccgccgaca cacagttcac cgacagctcc gatcggctgc agcattcccc atttatttaa
     7801 gataaatgcg gttgaattac caatcggccg gccgatcgga atggaatgtt catacagttt
     7861 ttcaatgtga aaacaagtcg aaaatgttgt attctcagtc ggcccatatc cgttccagat
     7921 tgacaattcc gggcatgctt gcttgattcg attgacatga ctggctgaca gagcttctcc
     7981 gccaataatc agatttatga gccgggaaaa tattttctca ttctgttctg ttaaatggtt
     8041 gaaaagagat gcagtcagcc acattgtagt gattccgttt tgttctatgt atctcttaag
     8101 ctgatgggaa tccagaaatg tttctttatc tgttagatgc aaagcagcgc catttagcag
     8161 cggcccgagt atttcaaatg taacgacatc aaaaccgagt gaagaagtca gcagaagccg
     8221 gtcttcaggt gtaaattcaa tgtaattgct gtttttgaca agacggatca cacttcgctg
     8281 ctcaatcatg acacccttcg gtttgccggt tgatcccgag gtatagatga tataagcgag
     8341 tgaatgtggc cctgctggag attcaaagtt cttcgtttct ccttgccctt gaatctcttc
     8401 gatgacgatt ttctcacatt taagctctaa gtctaatcgt tgattgtttg ctttttcaga
     8461 ttgaaggagt atttttgcac cgctgtcaga cagcatatat gatattcggt cagcgggaag
     8521 atccttagca ataggcagat aggctcctcc agctttcaaa atgccgagca ttcctgctgc
     8581 aatttctgga gttcgtgttg tcaataatgc aactacagtt tcaggtccga ccccttttct
     8641 ccgaagaaca gcagcaatcc tgtttgcccg gatgttcagc tccttatacg ttaatgtttg
     8701 ttcatcgccg gccacagcta tggcatcagg cgtttgtgca gcctgtaact caaacagcct
     8761 gctgatggtt tcatgtctcg gatactctct ttttgtacag ttgagttcat ggataaggaa
     8821 atgggtttca ttctcgtcca atacatgtat tgttccaagt gctgcttcgc tgtcttggat
     8881 gatggcgcgc agcatattca ttaaatagtg aagccacatt tcaatcgtat ttttctgata
     8941 gagatcagtg ctatattcca tttgaaacat catagaacca ttagcttcct cgtacgcata
     9001 tagggttaaa tcgaactttg agacctgatg ttccacattt gttgcgggcc ggagacacag
     9061 gtccttcagc tgcatggatt cttggtccat attctgaagc acaaacatca catcaaacag
     9121 cggatttcgg ctcatatccc ttgtcatgcc gagcttatcg acaagctctt cgaacggata
     9181 atcctgatgt tcgtaagctt ccaatgcagt ctcgcgcact tcctgcggca gata
//
