LOCUS       NZ_NUOG01000086        18904 bp    DNA     linear   CON 31-JUL-2024
DEFINITION  Bacillus sp. AFS033286 AFS033286_82_A2_Contig41_140604, whole genome
            shotgun sequence.
ACCESSION   NZ_NUOG01000086
VERSION     NZ_NUOG01000086.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus sp. AFS033286
  ORGANISM  Bacillus sp. AFS033286
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 43921)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 43921)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Assembly Cell v. 4.2.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 78.73x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002583065.1-RS_2024_07_31
            Annotation Date                   :: 07/31/2024 14:02:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,247
            Genes (coding)                    :: 4,997
            CDSs (with protein)               :: 4,997
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 83 of 250
            Pseudo Genes (incomplete)         :: 197 of 250
            Pseudo Genes (internal stop)      :: 59 of 250
            Pseudo Genes (multiple problems)  :: 74 of 250
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:15:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 25017
            Orig. end                         :: 43921
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NUOG01000086.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..18904
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..18904
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..18904
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[10000:13912](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..13912
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            860..1741
                     /locus_tag="COE07_RS26160"
                     /old_locus_tag="COE07_26160"
     CDS             860..1741
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018764967.1"
                     /locus_tag="COE07_RS26160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26160"
                     /product="glycosyltransferase family A protein"
                     /protein_id="WP_098841513.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.7e-27,
                     bitscore: 87.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLTVFTATYNRGYIIGELYESLKKQTYNDFEWLVIDDGSTDNTE
                     ELFKLWVSEESEFQIRYYKVINGGKHRAINKATDLANGELFFIVDSDDYLVEGALETVV
                     KWERELQGNSVFCGISGNKGKSENEILGTTFEGEYLDATSLERKEYKIDGDKAEVFYTH
                     ILKEYKFDEYEGEKFLTERTVWDRMAYDGYKIRWFNKIIYVCDYLEDGLTKSGDDLYAE
                     NPQGTSCIIKQQIKFYNYNIKERLSCYNSYYNLVKRNIGLTQAAKYLEIPKGTLVWAII
                     LVKCKDLLLFRT"
     gene            1906..3450
                     /locus_tag="COE07_RS26165"
                     /old_locus_tag="COE07_26165"
     CDS             1906..3450
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264124.1"
                     /locus_tag="COE07_RS26165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26165"
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_098841514.1"
                     /sec_met_domain="Polysacc_synt (E-value: 3.7e-17, bitscore:
                     54.0, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVKNSVINISVGLGNQIIITSLSFISRTVFIRTLGVEYLGINGL
                     ITNILAMLALVEAGIGASIMYSLYKPVADNDQNKINVLMKLYRNAYMIIALIVLLLGLS
                     IMPFLDYFLKDTTVENIHLIYSIFLFNTVAPYLYLHKNSFLNICQKNYIVTGVYSISSI
                     ISMCLKISILYYTKNYILYLIVDSVITIVTTIILVSIVNKMYPFLKDKTSEKLDYKTKK
                     DITKNIRAIVLQNIGNYLVFGTDNIIISSFVSVAAVGLYSNYNMLIEICRTFINQIFNN
                     IYHSIGNLVASETVDKVYTVYKVYRFLNFWLYSSFTISLYIMVEPFISIWLGSEFLMNK
                     SVLLILMITFYERGMRNSITTVKTTSGIFHEDRFAPLCQAAINLIFSLILVQYIGIVGV
                     FIGTLISALTVPFWLTPYLVYKKVFYKPVRIYFLNYVFYVFLGLGTYFVTNFISGEIVV
                     NSIFSLMLKGLLCVVVPNFIYICVFYSSDEFKYLLGIFRKIVGVVFVRFKKKKLEL"
     gene            3546..5984
                     /locus_tag="COE07_RS26170"
                     /old_locus_tag="COE07_26170"
     CDS             3546..5984
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152738.1"
                     /locus_tag="COE07_RS26170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26170"
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="WP_098841515.1"
                     /transl_table=11
                     /translation="MTFKANLEDLDNLDLITQSPVNGDVLTYDSTRAKWVPKKSVFNTN
                     TIVDQSNSYVIELDKWGIKNDGTNPLATTQGINEAIVWAKASGYNHIILPSGIYSLRIN
                     STSFSCIIMQSGIHFEMADGCNLQLENNSSPWYRIFEIKNVRNVKISGGKIIGDKKGHI
                     YELGVKFVRGGVNADGSLNNNPNFIRSEVLDRNEQPGILKVFRLWKIPNVVTSAYSFYQ
                     YKDTISSSTLVGVRTNGQFAPASPTGRGWFAEIEKCNKMIFVIDITSSPLSDEQISKIS
                     AKVDSQNYTHEWGQGIEILGSHQIEIENIEISDCTGDAISTGWLQYKLNPSEYTQEDMG
                     SHIYIYNCNIHHCRRQGVTLGASNDTYVYKNQIHHIGKNDDGVTSDFRSGIAPMFGIDI
                     ESMVGESNIPYKFPYHKQDGLEVNFRLFIFNNYIHDNERGHFVNNDGNYVTLENNIFEG
                     YNIGSVSSYQKTMEIKYLNNSFINCSLIVKGNHLVDGANFNNGQCRMLDVEGAVVRNIQ
                     VKEGAFLVSSVYGYLGTPSVNVTTSTFTYSQPHEMGNTAKVCFEQWVGRVPAGIDVNKL
                     YYVVNRTDTSFQVSEAIGGAPVVITDAGETGFNISRYNYGRCYVENVLVERDWRPNTAV
                     DVGCTLLMTGGVVKNITVKNYDIDIKPPYNYVGRPITLNDLTVIEGATNIECCNINNGS
                     FIKAKAKRLGGDITFGSNDVKYMRRINANNCFFESIDVNLEGNVILANSTFVKSGIGKS
                     ASGLNSSMLLNSYLENSKINLYWLIKKNHMTIAKCTFNNVIISVRDGVRMIDNLDVSNN
                     "
     gene            6249..6854
                     /locus_tag="COE07_RS26175"
                     /old_locus_tag="COE07_26175"
     CDS             6249..6854
                     /EC_number="2.7.8.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bac_transf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1146: sugar transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002070010.1"
                     /locus_tag="COE07_RS26175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26175"
                     /product="sugar transferase"
                     /protein_id="WP_098841516.1"
                     /sec_met_domain="Bac_transf (E-value: 1.1e-67, bitscore:
                     218.4, seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRTFDLCISLFLLLVCLLPILVVAILVRLKLGSPVLFKQQRPGL
                     HGKPFNLYKFRTMLDEKDSNGELLPDQDRLTTFGANLRKYSLDELPQLINVVMGDLSLV
                     GPRPLLMEYLPLYSSKQAKRHNVKPGITGWAQINGRNSISWDEKFKLDVWYVENQSFLL
                     DLKILYLTISKVLKSEGITNNKHVTMPIFKGKIERGGE"
     gene            6841..7818
                     /locus_tag="COE07_RS26180"
                     /old_locus_tag="COE07_26180"
     CDS             6841..7818
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002165241.1"
                     /locus_tag="COE07_RS26180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26180"
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="WP_098841517.1"
                     /sec_met_domain="ATP-grasp_3 (E-value: 8.8e-26, bitscore:
                     82.3, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGENEMNILFTSSGRRVSLIKQFKDALKQEGIKGNIFTADLKETA
                     PTIYFSHKHFIVPRVNEEGYIDELLKICKSEMINLIIPLIDTELILLANNSKVFEGIGV
                     KVLVSSMELNKIAHNKKYTYNFFVSNNIPTPKVYSDEEIERKEYQFPLLIKPFDGSSSK
                     GVTKIMNEKELEFFKEYIPNAMIQEYVSGEEYTIDVMVDFYGNIKTIVPRLRIETRAGE
                     VSKGMTKKDIDIIKAAETVIKALPNPIGCITLQCFKKEDGQITFIEINPRFGGGIPLSI
                     EAGANFPLWTIEMCQGEMFTEQDFNWRENLTMLRYDEAVFMESI"
     gene            7820..8515
                     /locus_tag="COE07_RS26185"
                     /old_locus_tag="COE07_26185"
     CDS             7820..8515
                     /EC_number="3.1.3.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016086741.1"
                     /locus_tag="COE07_RS26185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26185"
                     /product="HAD family hydrolase"
                     /protein_id="WP_088229998.1"
                     /sec_met_domain="HAD_2 (E-value: 3.6e-27, bitscore: 87.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVIKAIVFDMDDTLYKEKDYVVSGFKAVDDWIKENYKKIGFYNIA
                     IQLFDSGERKFVFNKTLEKLNIDYDEKLISNLIEQYRSHKPDIQLLEEADWVLNNLINN
                     VKIGLISDGYLVAQERKINALKLKERFHSIILTDKLGREYWKPSQIPYEKISKELQVPH
                     QQCVYIGDNLSKDFITAKKLNWITIHINREDGIYRNLIVEQDYKAHYTINNLRRLPDIP
                     VLKHMFLDT"
     gene            8547..9701
                     /locus_tag="COE07_RS26190"
                     /old_locus_tag="COE07_26190"
     CDS             8547..9701
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (42-166). E-value:
                     2.2e-07. Score: 22.3. Matches aSDomain:
                     nrpspksdomains_COE07_RS26190_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557690.1"
                     /locus_tag="COE07_RS26190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26190"
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="WP_088229997.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 2.3e-97,
                     bitscore: 317.8, seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.5e-07, bitscore:
                     22.3, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQTVQHKKRIYLSSPHMSGNEQKYIKDAFDSNWIAPLGPNVDGFE
                     RELASFVGVKGGAAVSSGTAAIHLALRLLNVQKGDTVFCSSFTFIASANPIVYLGAEPV
                     FIDSEPETWNMSPQALALALHDADKAGKLPKAVILVHLYGQSAKLDEILSLCNHYDVPI
                     IEDAAESLGSTYKGKASGTFGKFGVYSFNGNKIITTSGGGMLVSDDVEALERARFLATQ
                     AKDPAPHYEHSEVGYNYRMSNILAGIGRGQLEVLEDRVRARKSIYKRYYEALSFIPGFY
                     FMPELENTRSNRWLTTLTIDEKESGISIGKLLRDLEVENIEARSMWKPLHTQSLFKKKK
                     YYPHSKNEDVSQHLFQSGICLPSGSNMLVEDQQRVIQGVLKALK"
     aSDomain        8673..9044
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_COE07_RS26190_Aminotran_1_2.1"
                     /evalue="2.20E-07"
                     /label="COE07_RS26190_Aminotran_1_2.1"
                     /locus_tag="COE07_RS26190"
                     /protein_end="166"
                     /protein_start="42"
                     /score="22.3"
                     /tool="antismash"
                     /translation="GFERELASFVGVKGGAAVSSGTAAIHLALRLLNVQKGDTVFCSSF
                     TFIASANPIVYLGAEPVFIDSEPETWNMSPQALALALHDADKAGKLPKAVILVHLYGQS
                     AKLDEILSLCNHYDVPIIED"
     gene            10001..11995
                     /locus_tag="COE07_RS26195"
                     /old_locus_tag="COE07_26195"
     CDS             10001..11995
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002199626.1"
                     /locus_tag="COE07_RS26195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26195"
                     /product="lasso peptide isopeptide bond-forming cyclase"
                     /protein_id="WP_088229996.1"
                     /sec_met_domain="GATase_7 (E-value: 1.3e-21, bitscore:
                     68.2, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 1.8e-25, bitscore:
                     81.7, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAITGIIHFNNEPVSIEHGTRLMSDLQKYPADDIQIWRKENAFL
                     GCHAQWITPESVGEQVPFYNYEKQLAITADAIIDNRDELFEKLQVEYADRKNMTDSELI
                     LLSYEKWGEAAPKYLVGDFVFMIWDEKKQILFGARDFSGSRTLYFYRSENKFAFCTIIK
                     PLFTLPYVEKTLNEQWIAEFLAIPVNFESVDPQLTVYKYIEQVPPSHTVLVKDGKVEFS
                     RYYIPTAGKMLNLKSNEEYEEAFREVYQSAVKARLRTHHEVGAHLSGGLDSGSVVSFAA
                     KDLRAENKKLHTFSYVPAEGFEDWTHKGRIADERPFIQSTVQYVGNIQDYYLELPERSP
                     LSEIDDWLETMEMPYKFFENTFWLRGVYEKASQRDIGVLLSGQRGNWTVSWGPMLDYQA
                     MLLKKLHWIRFYRELHLYSRNLGVKKARVFEVVRRKAFPFFHQLLSSEEQDVFPIIINP
                     EFAKKMNVFDRLKEQDVDITGTSISTAYDMKREQFEKPYYWSINGTYETKLSLRYALWD
                     RDPTNDLRIIQFCLSVPEEQYVQNGLDRSLIRRATKNFLPDKVRLNQRVRGVQGADGVY
                     RMAPFWNEFIEEVQELSVDPIISEFLNVEVIKKAISKICKEPRSEYAFDLDFRILMRSL
                     IFYRFIKKINMKGGDTYEKGMETTCIRST"
     gene            11955..12080
                     /locus_tag="COE07_RS27695"
     CDS             11955..12080
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002172970.1"
                     /locus_tag="COE07_RS27695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="paeninodin family lasso peptide"
                     /protein_id="WP_140417308.1"
                     /transl_table=11
                     /translation="MKKEWKQPVLEVLNINMTMAGPGHRLPDAVQPDPDDPVKYS"
     gene            12160..13131
                     /locus_tag="COE07_RS26200"
                     /old_locus_tag="COE07_26200"
     CDS             12160..13131
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108921.1"
                     /locus_tag="COE07_RS26200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26200"
                     /product="aldolase"
                     /protein_id="WP_088229995.1"
                     /transl_table=11
                     /translation="MIDSRGNVLYKAFGLKIVSEIPLPELSQMNEKKEMADVQIEITDL
                     SERWDRYDESQQKTIVVEDDVVMFHVPQTAIFSIQAGEKITFSPMPEAKEDKIRLYILG
                     TCMGAILMQRKILPLHGSVIAIDGKAYALIGDSGAGKSTLASAFLNAGYQLLSDDVVAV
                     SLLNKNNAVVHPSYAQQKLWQESINELGMEVAQFSPLFERETKYAVPVNSQFFPDPLPL
                     AGVIELVKTQGEDIEMIRIEGLTRLYKLYQHTFRNSLISKLGLMEWHFETSSNVASGME
                     MFQLRRPDSKFTAHELVQVILNTIQKGSMVDGCSFEKHIIRT"
     gene            13094..13402
                     /locus_tag="COE07_RS26205"
                     /old_locus_tag="COE07_26205"
     CDS             13094..13402
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002128586.1"
                     /locus_tag="COE07_RS26205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26205"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_098252571.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 2e-28,
                     bitscore: 89.8, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 4.3e-17, bitscore: 53.7,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVHSRNISLEHNVVQSEGNIVSDMDGEKVMLNVQKGNYYNLGEI
                     GGEIWERIEKPIKINQLVTMLVSNYEIERKDCEEQVLSFLECLFQEGLIQIKDQSNR"
     aSDomain        13133..13381
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_COE07_RS26205_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="1.30E-28"
                     /identifier="RREFam002.1"
                     /label="COE07_RS26205"
                     /locus_tag="COE07_RS26205"
                     /protein_end="96"
                     /protein_start="13"
                     /score="89.8"
                     /tool="antismash"
                     /translation="VVQSEGNIVSDMDGEKVMLNVQKGNYYNLGEIGGEIWERIEKPIK
                     INQLVTMLVSNYEIERKDCEEQVLSFLECLFQEGLIQI"
     gene            13439..13912
                     /locus_tag="COE07_RS26210"
                     /old_locus_tag="COE07_26210"
     CDS             13439..13912
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012261438.1"
                     /locus_tag="COE07_RS26210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26210"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_098252572.1"
                     /sec_met_domain="PF13471 (E-value: 7.8e-25, bitscore: 78.7,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIVKRLRVFLSLNMETKLLFLEAFIFLGWARVLKSITFSKVAPS
                     LGDYMNETSVSQIQPHGDTLKKVSEAISIMSRYTFWESQCLVKAIAGMKMLEKRHIEST
                     LYLGTAKDSHGELIAHAWLRSGSFYVTGLEGMEKFTVVGSFAKRLSEDTIKGE"
     gene            13914..15101
                     /locus_tag="COE07_RS26215"
                     /old_locus_tag="COE07_26215"
     CDS             13914..15101
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_5"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409755.1"
                     /locus_tag="COE07_RS26215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26215"
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_098252573.1"
                     /sec_met_domain="NTP_transf_5 (E-value: 8.7e-68, bitscore:
                     219.9, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKDFGLDVEFMSKELKLLLAILKLQDDETVQTYSNEWFSDIDWE
                     VFLEQAVHHRVYPVIYQKLKQVDEELIPSNIVQTLNRKYKKNTFHMLYLSAEMERINKL
                     CNENEIRMIFLKGPVLSQDLYGDISLRTSCDLDVLIPMRNLEKVEMLLLEQGYVKDDYI
                     QTILNDWKWRHHHITFFHPTKQIKLEIHWRLNPGPGKEPGFEELWKRKRKSTLTSNSVY
                     MLGEEDLFLFLVSHGARHGWSRLRWLVDIKKIVDQDLNWSEIYKLMRKYHMLHLGGQAL
                     ILAAQLLGVRLDERMMELVAKKHSKQLAQEALFYFKQMINLHTEPVPEEVFIYHSRHLF
                     SLMSNQQKILYIMSQLYPYPIDAKTLPLPKFLHFLYFPLRPFLCLWRKTKKLALL"
     gene            15113..16912
                     /locus_tag="COE07_RS26220"
                     /old_locus_tag="COE07_26220"
     CDS             15113..16912
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016096316.1"
                     /locus_tag="COE07_RS26220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26220"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_098841518.1"
                     /transl_table=11
                     /translation="MNHIIYFMKKLYSSAGNILYVNLFGMVLISLLEGIGIILLIPMIS
                     MTGIINIGEETTFIAPVSRFLQDFPKTTSLASILGIYILIVLGQNLLQRNITLRDARTQ
                     QAFVRELRIETYSMILKAKWSFFLEKRKTNLINILTTELARVSYGVNLILQLLAAILFT
                     FIQVGIAFLLSPQITIFVLVFGLLFLVASRVFIKKARILGGKTSELAKDYLSGITDHFN
                     GIKDIKSNTLESSRLDWLQSITEKMSNEQMEYMRIRSNSQLFYKVSMAILIAFFLLLSV
                     SMFQAQSTQLLLIILIFSRLWPRFMTIQSNLEQLAASIPAFKSLWELQEECKEAIEMQD
                     IHQQKHIEPICIKQGIECKDVYFRYNKQESLYALQNINVQIPINSMTAIVGHSGAGKST
                     LIDVLMGLIQPEKGQVLIDGTLLTTDNLLSLRQVISYVPQDPFLFNASIRENLLMIDSN
                     ASEEQIWSALEFAAATEFVRMLPNGLDTLIGDRGVRLSGGERQRLVLARAILRKPSILV
                     LDEATSALDTENEVKIQSAIERLKGTMTIVVIAHRLSTIRNADQVIVLGKGEIVQKGEF
                     NQLAKERKGVFRNLLGSQMEVGS"
     gene            17141..17464
                     /locus_tag="COE07_RS26225"
                     /old_locus_tag="COE07_26225"
     CDS             17141..17464
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016105867.1"
                     /locus_tag="COE07_RS26225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26225"
                     /product="PadR family transcriptional regulator"
                     /protein_id="WP_140417307.1"
                     /transl_table=11
                     /translation="MNVQFKKGVLELCVLALVKRKDSYGYELVQQISNKFLISEGSVYP
                     LLRRLTKEGYFQTYLKESTEGPPRKYYQLTEQGEMQLKLLVTEWRDFAKGVQEIIEGVN
                     WDE"
     gene            17457..17999
                     /locus_tag="COE07_RS26230"
                     /old_locus_tag="COE07_26230"
     CDS             17457..17999
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031379.1"
                     /locus_tag="COE07_RS26230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26230"
                     /product="DUF1700 domain-containing protein"
                     /protein_id="WP_098252575.1"
                     /transl_table=11
                     /translation="MNKEQFLKELSNQLRKLPEEERQDILFDYEEHFQFGIEEGKSESE
                     IIKGLGSPKAIAKELLAMYRFDEMKKDPSTSNVTRAVMAAIGLSLFNFIIVLGPLVAII
                     AFIFSFWVGGIASVVTPFFVIVKVFMGTFIWLDLFVSITFVGVGLLLCIIAYYSTKWFK
                     KLCVRYVIWNFKMIKGE"
     gene            18000..18803
                     /locus_tag="COE07_RS26235"
                     /old_locus_tag="COE07_26235"
     CDS             18000..18803
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016512438.1"
                     /locus_tag="COE07_RS26235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_26235"
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="WP_098841519.1"
                     /transl_table=11
                     /translation="MMKKILLVAIACIIVGVIGISQTYKKAVANVETGNKEDVIKNEAL
                     KNVEVDLAAGDVVIQKSPDSSFYVKQTGDVRKQEIRIVEEGDALKIIGGREKGDSFDFS
                     FYNFRFQTPTVTIFVPERFYQEIKVKSSAGQIEISDVKSDRVRVETLAGEVELKHVTAK
                     NVEGSTKAGEVNFEKVIGKVTAKTASGDVEIIDHDSKYDLEASSTAGDIDITLLEKPQN
                     AVISGQSAAGDVTIFNEENRNVTIGNGSTKINGKTAAGDVTIETR"
ORIGIN
        1 ggcgagatga tagagtaata gtaaaacata ttaataatag tggagtatcc aatgcgcgaa
       61 atttaggtat aaagtatgca actggagaat ggatactatt tctagatggt gatgatactt
      121 tagagatgga tgtaatagaa aaggtttgca aaaacattaa aatgaatgaa agtatggaga
      181 tgttgattgg aagttttaaa tataaaaaaa aagatggggt tgtttatgct aacaataacg
      241 atttctatag taaaggaatt gatattgtgt gtgattatgg gttatggaaa attaaaatat
      301 gtatgggttc ttttgttgta aaaaaagaaa taatagaccg aaacaacatc tgttttcatg
      361 aaaggacaaa atatggggaa gatgtggaat ttattaatta ttgcttagta aactcagaaa
      421 aggtaaaagt aactatggat tactttatga gctacataat tcataatgaa tcagcaatag
      481 cgaaagttaa ttttgataga tatgattgct atgaatctag atgtcgttcg ttaaaatata
      541 ttcagcaaag ataccctaac tacatagata tagagttatt gtacaaaaaa tatttacttc
      601 cagaagcaat tattgataca acatatcttt tatgtggaag tggtgtgagt ctatttgcaa
      661 ttaaaaagta tttaaggaaa aacagttatt atcaagtcat agaggcagtt cagaaagatg
      721 agaatacgcc tttgtattta agaaaaaaaa ttaataaatt cttaaataat gcaatgctta
      781 catggggagc atgctttttg tcaaatcaat attactattt tagagggaaa ttaggagtga
      841 taaaaaggaa ggttataata tgaaattaac tgtttttact gccacttata atagaggata
      901 tataataggt gaattatatg aatcgttgaa aaagcaaacg tacaacgatt tcgaatggtt
      961 agttattgat gacggttcta ctgataatac agaagaactt tttaaattat gggtgtcaga
     1021 agaaagtgaa ttccaaataa ggtattacaa agtaataaat ggtggtaaac atagggcaat
     1081 taataaagcg acggatctag caaacggaga gttgtttttt atagtagatt ctgatgatta
     1141 tctagtagaa ggtgctttag aaacagtagt gaaatgggaa cgagaattac aagggaactc
     1201 agtgttttgt gggatttctg ggaataaggg aaagagtgaa aatgaaattt tggggacaac
     1261 gtttgaagga gagtatttgg atgcaacttc acttgagaga aaagaatata aaatagacgg
     1321 ggataaagcc gaagtctttt atactcatat tttaaaagaa tataagtttg atgaatatga
     1381 aggagaaaaa tttctcacgg aacgtacagt gtgggataga atggcttatg atggctataa
     1441 aataagatgg tttaataaaa taatttatgt atgtgattac cttgaagatg gtctaactaa
     1501 gtctggtgat gacttatatg ctgaaaaccc tcaaggtacc tcatgtatta taaaacagca
     1561 aattaaattt tataactata atataaaaga aagattatct tgctataatt cctattataa
     1621 tttagtgaaa cgtaatatag gattgacaca agctgcaaag tatttggaaa ttccaaaagg
     1681 cactttagta tgggcaataa ttttagttaa gtgtaaggat ctgttattgt ttagaacttg
     1741 atacattcta taggaaatta ttgtgcctat tctaaaatga gaatataggg gaagttgtag
     1801 gattattagc tttatttata atataaatta attacagctt atacagaaag tcattattgg
     1861 agtcataaat atatttccgt agaaaaagta tgggtgtggg aaaagatgag agtgaaaaat
     1921 tcggtaatta atatttcagt cggtttgggg aatcaaatta ttattacatc gttaagtttt
     1981 atttcgagaa ccgtttttat aaggacatta ggagttgaat atttagggat aaatggactg
     2041 attacgaata tattagctat gttagcgtta gtagaagcag gtataggtgc tagtataatg
     2101 tatagtctat ataagccagt tgcagataat gatcaaaata aaattaatgt acttatgaag
     2161 ttatatagaa atgcgtatat gataatcgct ttaattgtat tgctactagg attatctatc
     2221 atgccttttc tagattattt tttaaaagat acaacggtcg aaaatataca tttgatttat
     2281 agtatttttt tatttaatac tgttgcacct tatttatatt tgcataaaaa ttccttttta
     2341 aatatttgtc aaaagaatta tattgtaaca ggggtatact caatatcttc tataatttca
     2401 atgtgcctta aaatttcgat attatattat acgaaaaatt atattttata tttaattgta
     2461 gatagtgtga taacaattgt tactactatt atattggtaa gcatagtaaa taaaatgtat
     2521 ccatttttga aggataaaac ttcagaaaaa ttagattata aaactaaaaa agatattact
     2581 aaaaatatta gagccattgt cttgcaaaat atagggaatt acttagtgtt tggaaccgac
     2641 aatataataa tctcctcatt tgttagtgtt gcagcggttg ggctctattc caattataat
     2701 atgttaattg aaatctgtag aacttttatt aatcaaattt ttaataatat ttatcatagt
     2761 ataggtaatt tagttgcaag tgagactgtt gataaagttt atactgtata taaagtatat
     2821 aggtttctta acttttggtt atattcctct tttactataa gtttgtatat aatggtcgaa
     2881 ccgtttattt ctatctggtt aggttcggag tttttaatga ataaaagtgt gttattaata
     2941 ctaatgatta ctttttatga gagaggaatg agaaattcta taactacggt gaaaacaaca
     3001 tctggaattt ttcatgaaga tagattcgct ccactatgcc aagcggcgat taatttgatt
     3061 ttctctctta ttctagttca atatattggt atcgtagggg tttttatagg gacgcttata
     3121 agtgcattga cagttccgtt ttggcttact ccgtatttag tttataaaaa agtattctat
     3181 aaacctgtaa gaatttattt tctgaattat gtattttatg tgtttttagg tttagggaca
     3241 tattttgtta caaattttat aagtggagag attgtagtaa atagtatttt tagtttaatg
     3301 ctcaaaggtt tattatgtgt tgtagttccg aattttatat atatatgtgt gttttatagt
     3361 tcagatgaat ttaaatattt actggggatt ttcagaaaga ttgtaggagt agtatttgtg
     3421 aggtttaaaa agaagaaatt ggagctatag atggatttag taacgcattt gtgttcatct
     3481 atccaagccg ttatgcctct tattacatag gatatataaa agtaataaag aggtgataag
     3541 tttaaatgac ttttaaagct aatcttgaag atcttgataa tttagatttg ataactcagt
     3601 cacctgtaaa tggtgatgtt ttgacttatg atagcactag agcgaaatgg gttcctaaaa
     3661 aatcagtgtt taacactaat acaatagtag atcaatctaa ttcttatgtc atagaactag
     3721 ataagtgggg tataaaaaat gatggaacca atccgctggc tactactcaa ggaataaatg
     3781 aagcaattgt atgggctaaa gctagtggat ataatcatat tatattacct agtgggatat
     3841 atagtttaag aattaatagt acatcgtttt catgtattat tatgcaaagt gggattcatt
     3901 ttgaaatggc tgatgggtgt aatttgcaat tagaaaacaa ttcatctcct tggtatagaa
     3961 tattcgaaat taaaaacgta aggaatgtga aaattagtgg tgggaaaatt attggagaca
     4021 aaaagggaca tatttatgaa ttaggagtga aatttgttag agggggagta aatgcagatg
     4081 gctccttaaa taataatccg aattttattc gtagtgaagt tttggatcga aacgaacaac
     4141 cgggaatttt aaaagtattt agattatgga aaattccaaa tgtagtaact tcagcatata
     4201 gtttttatca gtacaaagat actatttcca gtagtacact tgtaggtgtt agaacgaatg
     4261 gacaatttgc tccagcatct ccaactggaa ggggatggtt tgccgaaata gaaaaatgta
     4321 ataaaatgat ttttgttatt gatattacat catccccctt aagtgatgaa caaatttcga
     4381 aaattagtgc aaaggtagac agccaaaatt atactcatga gtggggacaa gggattgaaa
     4441 tattgggctc acatcaaatt gaaattgaaa atatagagat aagtgattgt acgggtgatg
     4501 ctatttctac aggttggtta caatataaac ttaatccatc agaatatact caagaagata
     4561 tgggatcgca tatttatatt tataattgca atatacatca ttgtagaaga caaggtgtaa
     4621 ctttaggtgc ttcgaatgat acgtatgttt ataaaaatca aattcatcat atcggtaaaa
     4681 atgatgatgg tgttacatcc gactttagga gtgggatagc accgatgttt gggatagata
     4741 ttgaatcaat ggtgggagaa agtaatattc catataaatt cccttatcac aaacaagatg
     4801 ggttggaggt gaattttaga ctatttattt ttaataacta tatccatgat aatgaacgtg
     4861 ggcattttgt aaataatgac ggaaattatg ttactttaga aaataatata tttgaaggat
     4921 ataacattgg aagtgttagt tcttaccaaa agacaatgga aatcaaatat cttaataatt
     4981 cctttataaa ttgttcatta attgtaaaag gaaatcatct agttgatggt gctaatttta
     5041 ataatggtca atgtagaatg ttagatgtgg aaggagccgt tgtacggaat attcaggtga
     5101 aagaaggagc ctttttagta tctagtgttt atggatatct tggaacacca tcagtaaatg
     5161 tgacgactag tacatttacc tatagtcagc cacatgaaat gggaaataca gcgaaagtat
     5221 gttttgaaca atgggttgga agggttccag caggaattga tgtaaataaa ctatattatg
     5281 tggtgaatag aacagataca agttttcaag tatctgaagc aataggagga gcaccagttg
     5341 ttattacaga tgcaggagaa acaggattta atattagtcg gtataattat ggtcggtgtt
     5401 atgtggagaa tgtactggtc gaacgtgatt ggagaccaaa tacagcggtt gatgtagggt
     5461 gcacattatt gatgacgggt ggtgtagtga agaatataac ggtaaaaaat tatgatatag
     5521 atattaaacc accgtataac tatgtaggga ggccgataac attaaatgat ttaacagtta
     5581 ttgaaggtgc aactaatata gagtgttgta atataaataa tgggagtttt ataaaagcaa
     5641 aggctaaaag actgggggga gatattacat ttggttctaa tgatgtaaaa tatatgagaa
     5701 gaataaatgc aaataattgt ttttttgaaa gtatagatgt caatttggaa ggaaatgtaa
     5761 ttttggcaaa tagtactttt gtgaagtctg gtattgggaa atcagctagt ggtctcaact
     5821 cttctatgct tctaaatagt tatttagaaa atagtaaaat taatttgtat tggttaatta
     5881 aaaaaaatca tatgacgata gctaaatgta cttttaacaa tgttataatt tctgtacggg
     5941 acggagttcg tatgattgat aatttggatg tttctaataa ttaatcgata atagttattt
     6001 tatgacgggg tttacatggc tcaaaaatga atattgatta tgaagaaggc tactatgttt
     6061 gtttatcagc atagtggctt ttttattata agtttaacat aatgctcata atattagggg
     6121 atgttttaaa tcgaagattt gttttatatt ggaaattcca aatgattcat tgtgatagaa
     6181 atattgtatt taaagaaacg gctaaacgat tgaaaattta ggtgaaaagg aatgaggtcg
     6241 gagtattaat gaaaaggact tttgatctat gcatttcatt atttttatta ttagtatgtt
     6301 tgcttcctat tctagtagta gcaatcttag ttaggctgaa attaggatct cctgtactgt
     6361 ttaaacaaca gcgcccggga ctgcatggaa aaccgtttaa tctttataag tttcgaacta
     6421 tgctagatga gaaggatagt aatggggaat tacttccaga tcaagataga ctgactactt
     6481 tcggggcaaa tcttagaaaa tatagtttag atgagttacc tcaacttata aatgtggtaa
     6541 tgggtgattt aagtttagta ggcccacgac cattattgat ggaatactta cccctatatt
     6601 ctagtaaaca ggctaaaagg cataatgtaa aaccaggaat tactggatgg gctcaaatta
     6661 atggaagaaa tagtatctca tgggatgaga aatttaaatt agatgtatgg tatgttgaga
     6721 atcaaagctt tcttttagat ttgaaaatat tatatctaac tattagtaag gttttaaagt
     6781 cagaagggat aacaaataat aagcatgtga ctatgccgat ttttaaggga aaaatcgaac
     6841 gtgggggaga atgaaatgaa tattttgttt actagctctg gtaggagggt ctcattaatt
     6901 aaacaattta aggatgcgct taaacaagaa ggaatcaaag gcaacatttt tactgcggat
     6961 ttaaaagaaa ctgctcctac tatttacttt agccataagc attttattgt tccgcgtgta
     7021 aatgaagagg gatatataga tgaattatta aaaatatgta aatccgaaat gataaatcta
     7081 ataattccgt taattgatac agaattaatc cttttggcta ataattcgaa ggtttttgaa
     7141 ggtattggtg taaaagtact agtttctagt atggaattaa ataaaattgc tcataacaaa
     7201 aaatacacat ataacttctt tgtttctaat aacatcccca cacctaaagt atatagtgat
     7261 gaggagattg aaagaaaaga atatcaattt ccattattaa ttaaaccttt tgatggcagt
     7321 tctagtaaag gtgtaaccaa aataatgaat gaaaaggaat tggaattctt taaggagtat
     7381 atacctaacg ctatgataca agagtatgtt tcaggagagg aatatactat agatgtcatg
     7441 gtagattttt atgggaatat aaaaacgatc gttcctagat tgcgtataga gacacgagca
     7501 ggtgaggtaa gtaaaggtat gaccaagaag gatattgata ttatcaaagc agcagaaact
     7561 gtaataaaag cccttcctaa tcccattggt tgcatcactt tacaatgttt taaaaaggaa
     7621 gatgggcaaa ttacgtttat tgaaataaat cctcgttttg gtggaggaat tccgttatcg
     7681 attgaagcgg gagcgaattt tccactatgg acaattgaaa tgtgccaagg agaaatgttt
     7741 acggagcaag atttcaattg gagagaaaat ttaacgatgc ttagatatga tgaagcggtt
     7801 tttatggaga gtatttaata tggtgatcaa agcaattgtt tttgacatgg atgatacttt
     7861 gtataaagaa aaagattatg tcgtaagtgg ctttaaagcg gtggatgatt ggattaaaga
     7921 gaactataag aaaattggat tttataatat agcaattcaa ttattcgatt cgggggaaag
     7981 aaagtttgtg tttaataaaa cacttgaaaa gttaaatatt gattatgatg aaaaattgat
     8041 tagtaatttg atagaacagt ataggagcca taaaccagat attcaattat tagaggaagc
     8101 ggattgggta ctaaataatt taattaataa cgtgaaaata ggactaattt ccgacggata
     8161 tttagttgcc caagaaagaa aaataaatgc attaaagtta aaagagagat ttcattcgat
     8221 tattcttact gataagcttg gaagagaata ttggaagccg agccaaattc cttatgaaaa
     8281 aataagtaag gaactacagg ttccacatca gcaatgtgtt tatataggag ataatttaag
     8341 taaggatttt ataactgcga agaaattaaa ttggataaca attcatatta atagagaaga
     8401 tggaatttac cgtaatctta tagttgagca agattataaa gcgcattata caataaataa
     8461 tttaagaaga cttcctgata tacctgtatt aaaacatatg tttttagata cataatattt
     8521 taaatatgta tagaggatgt gtttacatgc aaaccgttca acacaaaaaa cgaatctatc
     8581 tttcctctcc acacatgagt ggaaatgaac agaaatatat taaagatgct tttgactcaa
     8641 attggattgc acctcttggt ccaaatgtag atgggtttga aagagaactt gcttcttttg
     8701 taggagttaa aggtggggct gcggtgagtt caggtactgc ggccattcat ttagctttac
     8761 gattattaaa cgttcaaaag ggggatactg tgttttgttc aagtttcact tttatagcga
     8821 gtgcaaatcc gattgtttat ttgggggctg aaccagtatt tattgattca gaaccggaaa
     8881 catggaacat gtcaccgcaa gctctagcac ttgcgttgca tgatgctgat aaagcaggga
     8941 aattacctaa ggctgtcatt cttgttcatt tatatggtca gagtgcgaaa ttagatgaaa
     9001 ttctatcgct ttgtaatcac tatgatgttc ccataattga ggatgcagca gaatctttag
     9061 gttctacata taaaggtaaa gcaagtggta cattcggaaa gtttggtgtt tattcattta
     9121 atggaaataa aatcattaca acttcgggcg gtggaatgct tgtttctgat gatgtggaag
     9181 cattagaaag agcgagattt ttagcgacac aagcaaagga tccagcacca cattatgagc
     9241 atagtgaggt agggtataat tatcgaatga gtaatatatt agctggtatt ggaagaggac
     9301 aattagaagt attagaagat agggtgaggg ctagaaagtc tatatataaa cgatattatg
     9361 aggcgctatc tttcatacca ggattttatt ttatgccaga attagaaaat actcgttcaa
     9421 atcgttggct cactacattg acaattgatg aaaaggaatc cgggatctct attggaaaat
     9481 tattgaggga tttagaggta gagaatattg aagcgcgttc tatgtggaag ccacttcata
     9541 cgcagtcgct ctttaaaaag aagaagtatt atccacatag caaaaatgaa gatgtatcac
     9601 agcatctatt tcaaagtggg atttgtttgc cttcaggatc taatatgcta gttgaagatc
     9661 aacaaagggt cattcaaggc gttttaaaag ctctaaaatg aaagaaataa aaagtaagta
     9721 aaagtgaaat tagatttgta tacgtacgac tgatagttct ttataatgaa ctatttgtga
     9781 aagttatttg agaatatcca gtttgaattg acataaaaag gatataatgc taggttttaa
     9841 atatataaac cttagtctac aaacgaatta aagtaaataa ttgagaaggt aataaaataa
     9901 gtaattttgt tcgttaaaaa gaatgataaa gaggtatgtt taacgaattt aatatataaa
     9961 tagtatttat agtaactttt catatattag gaggggaaaa atgagcgcta taactggcat
    10021 tattcacttt aataacgaac ctgtatcaat tgaacatggc acacgattaa tgagtgactt
    10081 acaaaagtat cctgctgatg atattcaaat atggcgtaaa gaaaacgctt tcctaggatg
    10141 tcacgcacaa tggattacac cagagtcagt tggtgaacaa gtacccttct ataattatga
    10201 aaaacaactg gcaattacag cagatgctat tattgataac cgtgatgagt tgtttgaaaa
    10261 attacaagta gagtatgcgg atagaaaaaa tatgacggat agtgagttga ttttgctctc
    10321 ttatgagaaa tggggagaag cagcaccgaa atatttggtg ggcgattttg tctttatgat
    10381 ttgggatgag aaaaaacaaa ttctgttcgg agctagagat ttttctggga gccgaacact
    10441 ttatttttat cgtagtgaga ataaatttgc tttttgtact atcataaagc cgttatttac
    10501 tctaccatat gttgaaaaaa cattgaatga gcagtggatt gctgaatttt tagctatacc
    10561 tgtaaatttc gagtcagtag atccacagct aacagtctat aaatatatag aacaggtccc
    10621 tccatcacac actgttttgg taaaagatgg aaaagtcgaa ttttcgcgat actatatacc
    10681 aactgctggg aaaatgctta atttaaaatc aaatgaagag tatgaagagg cgtttcgaga
    10741 ggtgtatcaa agtgcagtga aggcacgttt acgaacacat cacgaagtag gagctcattt
    10801 gagcggaggg ctagactcag ggtctgtcgt tagctttgct gcaaaagatt tacgtgctga
    10861 aaataaaaaa ttacatacgt ttagttatgt cccggcagaa ggatttgaag attggacgca
    10921 caaaggtagg atagctgacg aaagaccatt tattcaatcg actgtacaat atgtagggaa
    10981 tatacaggat tattatttag aattaccgga aagaagccca ttatcggaaa ttgatgattg
    11041 gctggaaaca atggagatgc cgtataaatt ttttgagaat actttttggt tgaggggagt
    11101 ttatgaaaaa gcatctcagc gtgacatagg agtactcctt agcggccaaa gaggtaattg
    11161 gacagtatcg tgggggccta tgttagatta ccaggcaatg ctgttaaaaa aattacattg
    11221 gattcgtttt tatcgtgaac tacatttata tagtagaaat ctaggagtga aaaaagctcg
    11281 tgtttttgaa gtagtaagaa gaaaagcttt tccgtttttt catcagttac tttcttcaga
    11341 ggaacaagat gtttttccta taataatcaa tccggaattt gcaaaaaaaa tgaatgtttt
    11401 tgataggctc aaagaacagg atgttgatat aacaggtact tctatctcaa ctgcatatga
    11461 catgaaaaga gaacaatttg aaaaaccata ttattggagc ataaatggaa cgtatgaaac
    11521 gaaattatcg ttacgttatg ctttatggga tcgtgatcct acaaatgact tacggattat
    11581 tcaattttgt ttgtcagtcc cggaagaaca gtatgttcaa aatggtttag atcgatcgct
    11641 tatccggaga gccacaaaaa atttccttcc tgataaagta cgattaaatc aacgcgtccg
    11701 aggtgttcaa ggtgcagatg gtgtttaccg tatggctcct ttttggaatg aatttattga
    11761 ggaagttcaa gaacttagtg tagatccaat aatctctgaa tttctaaatg tagaggtaat
    11821 taagaaagct atctcaaaaa tatgtaaaga acctcgatca gagtatgcat ttgatttaga
    11881 ttttagaatt ttaatgcgta gcttaatctt ctatcgattt ataaaaaaaa ttaatatgaa
    11941 aggaggtgat acgtatgaaa aaggaatgga aacaacctgt attagaagta cttaatatta
    12001 atatgactat ggcgggtcca ggacatagac ttcctgacgc agttcaacct gatccagatg
    12061 atccagtgaa gtatagttaa gtgattggaa aactaaagct cttatatatt tttattatat
    12121 aagagctttc aaaaaaattg acaatgtggg gtgaatagga tgatcgattc gaggggaaat
    12181 gtgttataca aagccttcgg gttaaaaata gtaagtgaaa tcccattacc cgagttgtct
    12241 cagatgaatg aaaagaaaga gatggcagat gttcaaatcg aaataactga tttatctgaa
    12301 cggtgggata gatatgacga atcacaacaa aaaaccattg tagtggaaga tgatgtagtg
    12361 atgtttcatg ttcctcaaac tgcaatattt tctatccaag cgggcgagaa aattactttt
    12421 tcacctatgc cagaggcgaa agaagataaa attcgattat acattctggg aacttgtatg
    12481 ggggcaattt taatgcaacg gaaaatactt ccattgcatg ggagtgtaat cgccatagat
    12541 gggaaagcgt atgcattaat tggtgattca ggagctggaa aatcaacgtt agcctcggct
    12601 tttttgaatg caggatatca acttttaagt gatgatgtag ttgctgtgtc tcttttgaat
    12661 aaaaataatg cagtagttca tccttcttat gcacaacaaa aattatggca agagagcata
    12721 aatgagttgg gaatggaagt agcgcaattt agccctttgt ttgaaagaga aacgaagtat
    12781 gctgttcctg tgaattctca atttttcccc gatccattac ctcttgctgg tgttattgaa
    12841 ttagtaaaaa cgcagggaga agatattgag atgatccgta tagaaggtct cacacgcttg
    12901 tacaagttat accaacatac ttttagaaat tctttgattt ctaaattagg attgatggaa
    12961 tggcattttg aaacttcatc taatgttgca agtggaatgg agatgtttca attaagacgc
    13021 ccggattcga aatttacagc tcatgagctt gtgcaagtta ttttaaatac tatacagaaa
    13081 gggagtatgg ttgatggttg ttcattcgag aaacatatca ttagaacata atgttgtaca
    13141 gagtgaaggt aatatagtaa gtgatatgga tggagaaaaa gttatgttaa atgtccaaaa
    13201 aggaaattat tataacttag gtgaaatagg cggagaaata tgggagcgta ttgaaaagcc
    13261 gataaaaatt aatcagcttg ttactatgtt agtatctaac tatgaaattg aacgaaaaga
    13321 ttgtgaagag caagttcttt cctttttaga gtgtttattt caagaaggtt taattcaaat
    13381 aaaagatcaa tctaatagat aaattcgtaa tttcaagaga actgttagga agggattaat
    13441 gaatattgtg aaaaggttaa gggtattttt atcgctgaac atggaaacaa agcttttatt
    13501 tttagaggct tttatttttt tagggtgggc gcgtgtacta aaaagtatta ccttttccaa
    13561 agttgcacct tctttagggg attacatgaa cgaaacatca gtttcccaaa ttcagccaca
    13621 cggggataca ctaaagaagg tgtctgaagc tatctctatt atgagtcgtt atactttttg
    13681 ggaaagtcag tgtcttgtta aagctatagc tgggatgaaa atgctcgaaa aacgtcatat
    13741 tgagagtact ctttatttag gaactgctaa ggatagtcat ggggaattaa ttgcgcatgc
    13801 atggctacgg agcggttctt tttatgttac tggattagaa ggaatggaga aatttactgt
    13861 cgttggatca tttgcaaaga ggctaagtga ggacactatt aaaggagaat aaaatggaga
    13921 aagattttgg tctggatgta gagttcatgt ctaaggaatt gaaacttctt ttggcaattt
    13981 tgaagttaca agatgacgaa acagtacaaa cttacagtaa tgaatggttt tcggatattg
    14041 attgggaggt atttcttgag caagctgtgc atcatcgcgt ttatccggtg atatatcaaa
    14101 aacttaaaca ggtggatgag gaactaattc cttcaaatat tgtacaaacc ttgaatagaa
    14161 aatataaaaa aaatacattt cacatgcttt atttaagtgc tgaaatggaa cgaataaata
    14221 aactatgtaa tgaaaatgag attcgtatga tttttctaaa aggacctgta ctatctcagg
    14281 acttatatgg ggatatttct ttacgaacat cctgtgattt ggacgttcta attccgatgc
    14341 gaaatttaga aaaagtagaa atgctacttt tagagcaggg gtatgtaaaa gatgattata
    14401 ttcaaacgat attaaatgat tggaaatgga ggcatcatca tataacattt tttcatccaa
    14461 caaagcagat taagcttgag attcattgga ggttaaaccc cggaccgggt aaggaacctg
    14521 ggtttgaaga actttggaag cgaaagagaa aaagtaccct tacgagtaat tctgtatata
    14581 tgttagggga ggaagattta tttttatttc ttgtatctca tggtgctaga catggttggt
    14641 ccagattacg ctggttagtg gatataaaaa aaattgtcga tcaagatctt aattggagtg
    14701 agatttataa gttaatgaga aaatatcaca tgcttcattt aggaggacaa gcattaattt
    14761 tagcagctca attgctaggt gtacgactag atgaaaggat gatggaattg gtagcgaaaa
    14821 aacattcaaa gcagttagct caagaggcgc tgttttattt caagcaaatg ataaatttgc
    14881 ataccgaacc tgtcccagaa gaagtattta tctatcatag tcgacactta ttttcactaa
    14941 tgtctaatca gcaaaagatt ttatatatta tgagccaatt atatccttat ccaattgatg
    15001 caaaaacatt accgttacct aaatttcttc attttttata ttttccttta cgtccgtttt
    15061 tatgtctttg gaggaaaaca aagaagcttg ctttgttata gggggaacat cgatgaatca
    15121 tattatatat tttatgaaga aattatattc ttcagcggga aacatactgt atgtgaattt
    15181 atttggtatg gttttaataa gtttacttga agggataggt attattttat taattcctat
    15241 gataagtatg actggaataa ttaatatagg cgaggagact acttttattg cacctgtctc
    15301 tagatttttg caggattttc ctaaaacaac aagtctagct tctattttag gtatatatat
    15361 actaatagtg ttaggtcaaa atttactaca aagaaatata acgctaaggg atgcgagaac
    15421 gcaacaagct tttgtacgag aattaagaat agaaacatat agtatgattt taaaagcaaa
    15481 gtggagtttt tttctagaga aaaggaaaac aaatctaata aatatattaa ctacagaatt
    15541 agctcgtgta agttatgggg ttaatttaat tttacaattg ttagctgcta ttctgtttac
    15601 atttatacag gtggggattg cgtttttatt atctcctcaa ataactatat ttgttttggt
    15661 ctttggctta ttattcttgg tagcttctcg tgtttttata aaaaaagcac gaatattagg
    15721 cggtaagacc tcagaattgg caaaagacta tctttctggt ataacagatc attttaatgg
    15781 aattaaagat ataaagagta atactttgga atcatcacgt ttagattggc tacaatctat
    15841 aacagaaaag atgagtaatg agcaaatgga gtatatgaga ataagatcga actctcaatt
    15901 gttttataaa gtttcaatgg ctatattaat tgcatttttt ttattattat cagtaagtat
    15961 gtttcaagcg caatccaccc aattactact aattatcctc atcttttcca gactatggcc
    16021 gagattcatg acaattcaat caaatttaga acaactggct gcaagtattc ctgcttttaa
    16081 gtcattatgg gagttgcaag aggaatgtaa ggaagcgatt gaaatgcagg atatacatca
    16141 gcaaaaacat attgagccga tttgtataaa acaaggtatt gagtgtaagg atgtttactt
    16201 tcgttataat aagcaagaat ctttatatgc acttcaaaat attaatgtgc aaattcctat
    16261 aaatagtatg acggccattg tggggcattc tggtgctgga aaaagtacat taattgatgt
    16321 gttaatggga ttaatccaac cagaaaaagg tcaagtatta atagatggta ctcttcttac
    16381 aactgataat ttattgtcat taaggcaggt tattagctat gtaccacaag atccgttctt
    16441 atttaatgct agtataagag aaaatttatt gatgattgat tcgaatgcaa gtgaagaaca
    16501 aatttggtct gcattagaat ttgcggcagc aactgaattt gtacgaatgc ttccaaatgg
    16561 gcttgatacg ttgattggag atcgcggggt gaggctttcg ggtggtgaac gtcagcgact
    16621 tgtattagct cgggccattt taagaaaacc gtcgatttta gtattagatg aggctacgag
    16681 tgcgttagat actgaaaatg aagtgaaaat tcaatcagca atagaaagat tgaaaggaac
    16741 gatgactatc gttgttattg cacatcgctt atctactata cgcaacgcag atcaagttat
    16801 tgtgttaggg aaaggtgaaa ttgtacaaaa aggtgaattt aatcaattgg ctaaggagag
    16861 gaaaggtgtg tttcgaaatt tattgggctc acaaatggaa gttggttcat agaggagcaa
    16921 gagtgaatga agttttgtgt tcatacagtt cgaatcgaat aaaagcccga gtaaaggtca
    16981 gttataacaa gatcttattc gggcttttat ttgtgcgttt tctaaattta ggaaataaat
    17041 ataaataggt accttgcaat agatagtacc aagtggtata ttatggataa aagggctacc
    17101 gtgtattgaa cagtactaag gtaagaaagg gggattgcaa ttgaacgttc agtttaaaaa
    17161 aggtgtgtta gaactatgtg ttttggcact tgtgaaacgt aaagatagtt atggttatga
    17221 acttgttcag caaatctcaa ataaattttt aatatcggaa gggtctgtgt atccattatt
    17281 gcgtcgttta acgaaagagg gatattttca aacgtactta aaagagtcta cagaggggcc
    17341 gcctcgtaaa tattatcagt taacagagca aggggagatg caacttaagc tattggtaac
    17401 agagtggcga gattttgcga aaggtgtaca agaaattatt gaaggggtga attgggatga
    17461 ataaagaaca gtttcttaag gagttatcga atcagcttag aaagttacca gaagaagagc
    17521 ggcaggatat tttgtttgat tatgaagagc actttcaatt cggaatagaa gaagggaaaa
    17581 gtgaatcgga aataataaaa gggctaggtt ccccgaaagc aattgcaaaa gaattgctag
    17641 cgatgtatcg atttgatgag atgaaaaaag atccgtcgac ttcaaatgtg acaagagctg
    17701 tcatggcagc gattggactt agtttgttta attttattat tgtgttaggc ccgttagttg
    17761 caattatcgc ttttatattt tccttttggg ttggtggtat tgctagtgta gtgacaccgt
    17821 ttttcgttat tgtaaaagta tttatgggca cttttatatg gttagatctt ttcgttagta
    17881 taacatttgt tggagtaggt ttgttgttat gtattattgc ttattatagt acgaagtggt
    17941 ttaagaaatt atgcgtacga tatgtcatat ggaacttcaa aatgataaaa ggggagtaga
    18001 tgatgaaaaa aatactgtta gtagctatcg cttgtatcat tgttggagta atagggattt
    18061 cgcaaacata taaaaaagca gtagcaaatg tcgagacggg aaataaggaa gatgttatta
    18121 aaaacgaagc gttaaaaaac gtagaagttg atttggctgc tggagatgtt gttatccaaa
    18181 aatctccaga ctcctctttt tatgtaaagc aaacagggga tgtgcgaaaa caggagattc
    18241 gtattgttga agagggtgac gcattaaaaa ttataggagg aagagaaaag ggtgattcgt
    18301 ttgatttttc attttacaat ttcagatttc aaacgcctac agtaactata tttgtacctg
    18361 aacgttttta ccaagagata aaagtaaaat catccgctgg acaaatagaa attagtgatg
    18421 tgaaaagtga ccgtgtaaga gtggagacac ttgctggaga agtggagttg aaacatgtaa
    18481 cagctaagaa tgtggaggga tctacaaaag ctggtgaggt gaattttgaa aaagtaatag
    18541 ggaaagtgac agcgaaaaca gctagtggag atgtagagat tattgaccat gattctaaat
    18601 atgatttaga ggcaagttca accgctggag acatagatat tactttattg gaaaaaccac
    18661 aaaatgcagt tataagtggg caaagtgctg ctggagatgt aacgattttt aatgaggaaa
    18721 atagaaacgt aacaattggt aatgggagta caaagattaa cggtaaaaca gctgctggag
    18781 atgttactat agaaacacgt taataaataa tatgtgcgtt tttgaatgga attctatcaa
    18841 aatataaaaa gaagataatt acctatataa ataaggtaat tatcttcaga gtgtttggaa
    18901 accc
//
