LOCUS       NZ_NUOG01000013        10267 bp    DNA     linear   CON 31-JUL-2024
DEFINITION  Bacillus sp. AFS033286 AFS033286_82_A2_Contig24_140604, whole genome
            shotgun sequence.
ACCESSION   NZ_NUOG01000013
VERSION     NZ_NUOG01000013.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus sp. AFS033286
  ORGANISM  Bacillus sp. AFS033286
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 365820)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 365820)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Assembly Cell v. 4.2.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 78.73x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002583065.1-RS_2024_07_31
            Annotation Date                   :: 07/31/2024 14:02:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,247
            Genes (coding)                    :: 4,997
            CDSs (with protein)               :: 4,997
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 83 of 250
            Pseudo Genes (incomplete)         :: 197 of 250
            Pseudo Genes (internal stop)      :: 59 of 250
            Pseudo Genes (multiple problems)  :: 74 of 250
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:15:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 220832
            Orig. end                         :: 231099
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NUOG01000013.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10267
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10267
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10267
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5267](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5267)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(434..447)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.04"
                     /tool="antismash"
     misc_feature    complement(463..479)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.0"
                     /tool="antismash"
     gene            complement(1036..1479)
                     /locus_tag="COE07_RS09545"
                     /old_locus_tag="COE07_09545"
     CDS             complement(1036..1479)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="COE07_RS09545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_09545"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_098252060.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDKANEKILMVKNKGKNGSYYTLPGGAVKFGETL
                     EEAVIREVKEETGLHISVNGICSISEAFFEERDHHAIFFNFSGEIIGGETYISRPKEIE
                     EITWMELHIAAPYLRIPEHLLDILQKKETVPYFFNGTIVHQST"
     misc_feature    1622..1633
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.51"
                     /tool="antismash"
     gene            complement(1671..2087)
                     /locus_tag="COE07_RS09550"
                     /old_locus_tag="COE07_09550"
     CDS             complement(1671..2087)
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016104977.1"
                     /locus_tag="COE07_RS09550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_09550"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_098252059.1"
                     /transl_table=11
                     /translation="MEIVQQWIKEDSDYIREKVIEYNQKHLSDDEKDPSEQVSFIVRNE
                     KEEIVGGITAITFWHHVHVDFLWVSEEYRHEGYGSELIKRIEEFAIEKGCRLINLDTFS
                     FQAPDFYKKHGYKVIGISEDHPKGHNHYYLEKRL"
     gene            complement(2221..3429)
                     /locus_tag="COE07_RS09555"
                     /old_locus_tag="COE07_09555"
     CDS             complement(2221..3429)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="COE07_RS09555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_09555"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_088229342.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAGKLQQKFGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLIDSLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNTVTETLHNDYTPREMMKTKQVYFLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAIFNTIGRIILGTLSDKIGRLKIVSA
                     TFIVIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGALLGGFQPTFITIGVLCVISFIISILIRPPSAETKKDSNQLHR
                     KIA"
     gene            complement(3708..3851)
                     /locus_tag="COE07_RS27850"
                     /old_locus_tag="COE07_09560"
     CDS             complement(3708..3851)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="COE07_RS27850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_09560"
                     /product="hypothetical protein"
                     /protein_id="WP_088229343.1"
                     /transl_table=11
                     /translation="MKENVSKNKKKNIKDVTTEQNAIYSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            complement(3883..4899)
                     /locus_tag="COE07_RS09565"
                     /old_locus_tag="COE07_09565"
     CDS             complement(3883..4899)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="COE07_RS09565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_09565"
                     /product="serine hydrolase"
                     /protein_id="WP_088229344.1"
                     /transl_table=11
                     /translation="MYTYDKLISWVENIKEKNHSSATALCIIKDNKIVLEHYSGYHSNT
                     SKSKKVTASSQFNVASARKSYLGLMVAYALYEGKINSIEDEAIQYFKDFNPLLLGKTTI
                     RHLVTHSHGLTETDDGTIFREFEPGQAWAYRDINVRMMTRLIYQLYNKSFPELLKERVF
                     KPAKFQETGWRIQQDENLVKVVNNPNEAAISEVGTVDDGTEKNLFVSAREFACWGNLHL
                     NQGMINSKQIVPKEVIKIATSLQSPNYKNNELPQNGLFWFVQNEPAQLSEIGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRFNYGGDNYLYYLREFSNLVADTFSSGNRA"
     gene            complement(5001..5267)
                     /locus_tag="COE07_RS09570"
                     /old_locus_tag="COE07_09570"
     CDS             complement(5001..5267)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071381.1"
                     /locus_tag="COE07_RS09570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_09570"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_140417262.1"
                     /sec_met_domain="TIGR03601 (E-value: 5.6e-49, bitscore:
                     159.2, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQSLNLNDYQSGNVVYWDQQQNQYPYYYIQDDARRCGG
                     CGGCGGRCGGCGGRCGGCAGRCGGCIGCAGCFSCFNCWNWWFI"
     gene            complement(5450..5896)
                     /locus_tag="COE07_RS09575"
                     /old_locus_tag="COE07_09575"
     CDS             complement(5450..5896)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="COE07_RS09575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_09575"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_088229346.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAVMIKDEKLLVAEYIGYHYFLPGGHVEVGESAENA
                     LIRELREELGVNCSIKQFLGVIENQWQDREVLHHEINHIFEIDSQELHIEFIPKSKESH
                     LAFHWIDYNQEALNDYKIMPAPSVKELLERKLSDELLNCWISNF"
     gene            complement(<5933..6028)
                     /locus_tag="COE07_RS09580"
                     /old_locus_tag="COE07_09580"
                     /pseudo=""
     CDS             complement(<5933..6028)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160776.1"
                     /locus_tag="COE07_RS09580"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_09580"
                     /product="cytochrome C biogenesis protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MYFTEVKIEVCIPEEYISLLRDELNKVDTCKT"
     gene            complement(6059..6517)
                     /locus_tag="COE07_RS09585"
                     /old_locus_tag="COE07_09585"
     CDS             complement(6059..6517)
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="COE07_RS09585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_09585"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_098252058.1"
                     /transl_table=11
                     /translation="MNIHLKVVTRENWRDALNLQVKKEQRNFVPSVAVSLAKVYIKPDG
                     DNVEYVPFAIYDGDHMVGFIMHAFARETTDMYWINGFIIDQKQQGNGYGKAALQESIYL
                     IKNTSMACKEIRLTVHKDNISAKKLYERYGFQSLGHDYDGEEVYRLFV"
     gene            complement(6628..7539)
                     /locus_tag="COE07_RS09590"
                     /old_locus_tag="COE07_09590"
     CDS             complement(6628..7539)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107015.1"
                     /locus_tag="COE07_RS09590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_09590"
                     /product="DMT family transporter"
                     /protein_id="WP_088229348.1"
                     /transl_table=11
                     /translation="MHNKKWDLRIICAHTLTILIWGTAFPAIRRGLESYTPEHLTLLRL
                     LIASFILLLFSFIYKLRLPDLKDIPAIFMFGALGFTIYHIALNYGEKTVNAGSASLIVS
                     VTPIVTAIIAFVFLNEKMKLNGWIGGVISFIGIAFISFSQGDSIQLKSGGLFILLAAVS
                     ESLFFVFQTSYLKKYGFLPFTIYTILSSTVCMLIFLPGIYQEILAAPLEVNLSVIYLGL
                     FPTVLPYIALAYIISHAGASEATSSLYLTPVTACFIAWVWLGEVPTFISVVGGVITILG
                     VLIAHVSLKKDIRSEKYSNING"
     gene            7692..9125
                     /locus_tag="COE07_RS09595"
                     /old_locus_tag="COE07_09595"
     CDS             7692..9125
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (114-464). E-value:
                     6.2e-36. Score: 116.2. Matches aSDomain:
                     nrpspksdomains_COE07_RS09595_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016122597.1"
                     /locus_tag="COE07_RS09595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_09595"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_088229349.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 3.7e-35, bitscore:
                     116.3, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTHKKTPKYQQIVDYIKEKIENGEWPIGSKIPSQRQLAKLFHVN
                     RSTVITALDEIMADGLIQGQIGVGTIVTNNTWSLLATNPPPDWSEYVKAGIHKPSKLMV
                     REINEAEANKKLIQLSKGELSPDLFPIETMQAIMKNVSTELDYFGYEEQKGFYPLREAI
                     STYVKTFGIHVSPNSILIVSGALQALQLISIGLLHRESTVLLEQPSYLYSLHVFQSANI
                     HLSGISMDHHGILPSDLLKRIKYSQKQNILYSIPCFQNPTGILMSKERRKEILKICEKE
                     QLPIIEDDIYRELWIDEQPPVPLKSMDKHGHVLYIGSLSKTLTPGLRIGWIIGPEPVID
                     RLSDIKMQTDYGSSSLSQRVAAEWINKGFYEDHVANVRIHLKKRRLMMIQALNEYCTDI
                     ATWDVPTGGVFIWIKIVPNIPMKKLFSEALSKGLLLNPGRIYEEKSDQCIRLSYGYASS
                     EQITNGIKLLSGLIRKLMA"
     aSDomain        8034..9083
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_COE07_RS09595_Aminotran_1_2.1"
                     /evalue="6.20E-36"
                     /label="COE07_RS09595_Aminotran_1_2.1"
                     /locus_tag="COE07_RS09595"
                     /protein_end="464"
                     /protein_start="114"
                     /score="116.2"
                     /tool="antismash"
                     /translation="KLIQLSKGELSPDLFPIETMQAIMKNVSTELDYFGYEEQKGFYPL
                     REAISTYVKTFGIHVSPNSILIVSGALQALQLISIGLLHRESTVLLEQPSYLYSLHVFQ
                     SANIHLSGISMDHHGILPSDLLKRIKYSQKQNILYSIPCFQNPTGILMSKERRKEILKI
                     CEKEQLPIIEDDIYRELWIDEQPPVPLKSMDKHGHVLYIGSLSKTLTPGLRIGWIIGPE
                     PVIDRLSDIKMQTDYGSSSLSQRVAAEWINKGFYEDHVANVRIHLKKRRLMMIQALNEY
                     CTDIATWDVPTGGVFIWIKIVPNIPMKKLFSEALSKGLLLNPGRIYEEKSDQCIRLSYG
                     YASSEQITNG"
     gene            complement(9171..9701)
                     /locus_tag="COE07_RS09600"
                     /old_locus_tag="COE07_09600"
     CDS             complement(9171..9701)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495179.1"
                     /locus_tag="COE07_RS09600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_09600"
                     /product="DUF4269 domain-containing protein"
                     /protein_id="WP_088229350.1"
                     /transl_table=11
                     /translation="MFTSITYLQSGNDKQQQVYNVLNKLNIMEDLSLYSPVLCGTIPIR
                     IDTPQSDLDIVMEVHHFNAFEQEMRSLYGSYEGFKIKKKKIKNTESIKVNFKFEGFEFE
                     LFAQPKPVRNQNAYKHMIVEHMLLMQHPYIREKIIHLKEQGLKTETAFAQVLKINGDPY
                     EELILLGQEMRLW"
ORIGIN
        1 atcgaccata aatcgtccat cgaaagatat atttaattgt gtttcacctt gaatcacatc
       61 tatttgttgt gtttcagcta ttttaccaac ttgagagtcg ttagaataga ttttaatagt
      121 ggattcgttg atgatttcta agtttacatt gttatttgcc cattcacttg ctaataaact
      181 cgatcgatct acgccttgta aaatcttttt tctatctatg tgaataaccg tttgaaattc
      241 atttggaatg agactagata tattaggata tttcccttca attagtcttg aatataacgt
      301 agttgtgccg aatgtaaaaa caatatgact ctctgaaaaa tagatatata cgagtttcga
      361 attactattc attaatttaa gtagttcact aatagttgaa ctaggtacta tacagtttgc
      421 cttcacattt gtagaaacta gtgtttcacg tatagcaagt ctatgcgagt cagttgcagc
      481 acaaattaat ttattatgat ctaactctat atgtacgccg gtaagaacgg gtctagattc
      541 atttttagcc actgcaaaaa cagtttgttt aaacacttca attaattgtt ctgtttctat
      601 ttgtatttct gtatgattgt ctattagtgg tacgttagga aactcatctg ctggaaatcc
      661 atttaagttt aaagtaattt ctgctgattg tatggtaatt gtttgctcat tcttactttt
      721 tattactata tcatttggca ttttttttat tatttcaatg aaatattttg caggtacaac
      781 aatagttcct gcttttaaga tagttgtaat ttgttcgtct tcgattgaaa tagggatgaa
      841 tttctcgata aaaatattcg aattactcgc gattaaagta attccagatt gatctgctgt
      901 tattttgata ccagataata taggaattaa agcttttgtc gatatagctt tacttacttc
      961 tgaaagtgct tgagtgaagt gtttatgatt aacgataaac tccatttttc ccccgcctta
     1021 tatgaaatga tatttttatg tagattgatg aacgattgtt ccgttaaaaa agtacggtac
     1081 tgtttctttc ttttgtaaaa tgtctagtaa atgttctggt atacgtaaat acggtgctgc
     1141 tatatgaagc tccatccaag taatctcttc aatttctttt ggccgggata tatatgtctc
     1201 tccgccgatt atctcgcctg aaaaattaaa gaaaatggcg tgatggtctc tctcctcgaa
     1261 aaaggcttcg ctgatagagc aaatcccgtt tacagatata tgtagtcctg tttcttcttt
     1321 cacttctcga attactgcct cttctaacgt ttccccgaat ttaaccgcac cgcctggtag
     1381 tgtgtaataa gaaccatttt tccctttgtt ttttaccatc aagatctttt cgttagcttt
     1441 atcgtacaaa agcgcgtatg taacattgac ttttttcata cgtacctcct ctaattttaa
     1501 taaaaggaat tttctatatt tatattattg tatcatttcc tcgtttcgat atataaatta
     1561 actaaaccct tcatttatcg ctataagtaa cgtctgctac atataaaaaa gaccttattt
     1621 tgaaaaggtt atccaaagta cggtcttttt attttttaat actacacact ttataatctt
     1681 ttttctaaat aataatgatt atgccctttc ggatgatctt cacttatccc aattacttta
     1741 tatccatgct ttttataaaa atcaggagct tggaaactaa aggtatctaa gttgatgagc
     1801 ctacatcctt tctcgattgc aaattcttca atacgcttta ttaattcgct tccgtaccct
     1861 tcatgtctgt attcttcaga aacccacagg aaatcaacat gtacatgatg ccaaaaggtt
     1921 attgctgtta tccctcctac tatttcttct ttttcgtttc taactataaa actaacctgc
     1981 tctgaagggt ctttttcgtc atctgaaaga tgtttttgat tatattcaat tactttttct
     2041 cgtatgtaat cactatcttc tttaatccat tgctgtacta tttccacttt taatatcacg
     2101 ctccttttat tttatttatt ccatttcata attgtattgt cctgtcttta ctgatcaatt
     2161 tacggagcaa agtttacgtt gtagtgaaaa agagccctag cataaggact ctttttaaaa
     2221 tcaagctatt ttccgatgta actgatttga atcctttttc gtttctgcac ttggcggacg
     2281 gattaaaata gaaatgatga aagagatcac acataaaaca ccaattgtta tgaaggttgg
     2341 ctgaaaccct ccaagtagtg caccaataaa tgaacctgca agtgcaccaa atccaaaacc
     2401 ttggtatacg ataccgtaat ttgtactatg gttttttaaa ccgaagaaat caccaacaat
     2461 ggctgggaat attgtgatgt taccaccgaa acaaaaagca acgcttgcta cacaagcgaa
     2521 atatatcccg taatttagtg ggataaaact tagtgtaaag actgacaatc ctataacaat
     2581 aaacgttgcc gagacaattt ttaatcgacc tattttatct gataacgttc caaggatgat
     2641 tctacctatt gtattaaaaa ttgcaatcat agcgacggcg ttagcagcag ttgctgcact
     2701 aagaccaacg agctggactc cgatatcttt taccatcccg attaaataca atccacccat
     2761 acacgacgta aatagcatga aaaacaagaa atatacttgt ttcgttttca tcatttcacg
     2821 tggcgtataa tcattgtgta atgtttctgt taccgtgttg cttacaattg cttcacgtaa
     2881 gaagaacgat ccaatcaata ctaaaagtaa aacgataata ccccaatata aaaatgcttg
     2941 tgatacacca agactatcga taagattacc atttatatat ctaaagatta aactccccat
     3001 tccatatgct gaaacagata taccagaaat aagcccttta cggtttggaa accatttaat
     3061 taaattagat agtgatgtaa tatatgctgt tccatcagca tatccaacga caacaccggc
     3121 caataaataa agtaatggta atgaggaaac ttgtgaacta agtattaaac cgagtcctaa
     3181 aacaattcct gcagtagcga taagtttacg aagaccaaat ttttgttgta gctttcctgc
     3241 aaatagagtt gaaaatgata aagaaaaact cgtaatagag aaagtaatcg cgactgaatt
     3301 gagattccat ccaaacttac ttacaagggg ttgattaaat aaactccatg tataaattgt
     3361 tcctaggcca atttgaacaa tgattgtacc tagaacaatt agcaacggat ttatagatgt
     3421 ttgtttcata cgtactgtcc ccttctttta tcatgacatg aattttcata gttagtatat
     3481 ccacgataaa atgcaagata ctgtacgaaa aatggtaatt aatttcatat acacaaaaag
     3541 acacttcata atgaagtgcc tttttgcagg ttatatcaga tatatttaaa gaaagctttg
     3601 ttaagataag acattttcaa atgcttatgt atatattacc aaatgatttc ttacatgtaa
     3661 tgggacttga gatgtatttt taattcatca tatagtcgga taagaaatta atattcaatt
     3721 tcatcaaaat ccttcggctg cggtaccgtc tgcatgtttg ctgcgtcttt tgggtcgcta
     3781 taaatcgcat tttgctctgt agttacatct tttatatttt tctttttatt tttgcttaca
     3841 ttttctttca cgttatatca ctcctttttg ttataggagt aattatgccc tattaccgct
     3901 actaaatgta tctgcgacta aattgctaaa ttcacgtaaa taatataaat aattatcacc
     3961 gccgtaatta aaccttttat tgtacatttt tgcaacaact acattatatt caggtatcac
     4021 taaaatggtt ggtccagtaa ttcctaatat ttgatacgaa ccttttggaa cacgttcacc
     4081 aatttcactt aattgtgcag gttcattttg aacgaaccaa aacaacccgt tttgtggtaa
     4141 ttcattattt ttataatttg gactctgcaa gctggtagca atttttataa cttcttttgg
     4201 aacaatttgt ttactattta tcataccttg atttaaatgg aggttacccc aacatgcaaa
     4261 ctctctagct gagacaaaca gatttttttc agtaccatca tctactgtac ccacttcgct
     4321 aatcgcggct tcatttggat tattaacaac tttaactaaa ttttcatctt gctgaattct
     4381 ccatcctgtt tcttggaatt tagcaggttt aaacacacgc tcttttaaca attcaggaaa
     4441 acttttgtta tatagctgat aaataagacg tgtcatcatt cttacattaa tatctctata
     4501 cgcccaagct tgtccgggtt caaattcacg aaaaattgtc ccgtcatccg tttccgttaa
     4561 gccatgcgag tgagttacta aatgtcttat cgttgtttta ccaagtaata aaggattgaa
     4621 gtctttaaaa tattgtatcg cctcgtcctc aatagagttt attttcccct cgtaaagcgc
     4681 atacgctacc attaatccta aataactttt tctcgcagaa gcaacgttaa actgagaaga
     4741 tgctgttacc tttttacttt tagatgtatt tgaatggtat ccactataat gctctagtac
     4801 aattttgtta tcctttataa tacaaagtgc tgtagcagaa ctatgatttt tttcttttat
     4861 attctctacc caagagatca acttatcata tgtatacaaa taaatcctcc ttaatacgca
     4921 taaaaatcac agtatgaaag aaaagcataa actagtattt acgcctttct ttcatatgaa
     4981 atattagtga tttactacaa ttaaataaac caccagttcc agcaattaaa gcagctgaaa
     5041 catccagcgc aacctataca tccaccgcaa cgacctgcac aaccgccgca tctgccgcca
     5101 caaccaccac agcgtccacc acaaccgcca catccaccgc agcgacgtgc atcgtcctga
     5161 atgtagtaat atggatattg attttgttgt tgatcccaat acacaacatt accagactga
     5221 taatcattta ggtttaatga ttgtagttct tgttgaaact gattcattac gaaaccctcc
     5281 atttataaat ataaattccg tccaaatatt aaaattaata tagaaacaag gctatagact
     5341 aaatgcgtac atcaacaaaa tatgaactta ctctaggtga tgtaccttgt tttttagcct
     5401 attttcttac tttccttttg gcggacggat ttactattga tagaggcgtt taaaaattgc
     5461 taatccaaca gtttagtagt tcatcactta attttctttc tagtaactct ttaactgaag
     5521 gtgctggcat gattttataa tcatttaaag cttcttgatt ataatctatc cagtgaaacg
     5581 ctaaatgaga ttctttagat ttcggtatga attcaatatg taactcttgt gaatctatct
     5641 caaaaatatg attgatttca tggtgaagaa cttctcgatc ttgccattgg ttttctatga
     5701 ctcctaaaaa ttgttttata ctacaattta ctccgagttc ttctcgtagt tctcttatta
     5761 atgcattctc agctgattcg ccaacttcaa catgaccacc tggtaaaaaa taatgatatc
     5821 cgatgtattc agcaactagc aatttttcat cttttatcat gacagcccgt acaatatgat
     5881 ggaatttgct tttcaatcta tattcctcct agtcattcaa tttaaaatag cacgttttac
     5941 aagtgtcgac tttatttaat tcatctcgta ataatgaaat gtattcttca ggaatacata
     6001 cttcgatttt cacttctgta aagtacatgt tttatttcac gcctttactt aacaaaagtt
     6061 aaacgaataa acgatatact tcttcaccat cataatcatg tcccaatgat tgaaaaccat
     6121 aacgttcata tagtttcttt gcagagatat tatctttatg tactgttaat ctaatttctt
     6181 tacacgccat acttgtattt tttattaaat aaatactttc ttgtaatgct gcttttccat
     6241 aaccattacc ttgctgcttt tgatcgataa taaatccgtt tatccaatac atatcagttg
     6301 tttctcttgc aaaagcatgc ataataaaac caaccatatg gtcgccgtca tatatcgcaa
     6361 atggtacgta ctctacattg tcaccatctg gttttatata tactttagca agtgaaactg
     6421 ctactgatgg aacaaagttt ctttgctctt ttttaacttg taaatttaat gcatctctcc
     6481 aattctctct cgtaacaacc tttaagtgga tattcaatat tgtaagctcc tttattaaga
     6541 ggatatattt ttatattcaa cgctgtaaaa taattccctg tttcttgatt ttaagaaatg
     6601 tgttgaatta tcacttctta aaaaaatcta tccattaata ttgctgtact tttcacttcg
     6661 aatatctttc tttaatgata catgtgctat taaaactcca agtatagtaa ttaccccacc
     6721 aactaccgaa ataaaagtag gtacttcacc taaccatacc caagctataa aacacgcagt
     6781 tactggcgtt aaatatagag aacttgttgc ctcggaagca ccagcatgag atataatata
     6841 tgccaatgca atatacggga gtactgttgg aaataagcct aaatatataa cgctcaaatt
     6901 aacttcaaga ggagctgcta gtatttcttg ataaattccc ggtaagaaaa taagcataca
     6961 taccgtacta gataaaattg tatatatggt aaatggcaag aagccgtact tttttaagta
     7021 agatgtttgg aaaacgaaaa ataggctttc tgaaacggct gctaataata taaataaccc
     7081 tccacttttt aactgaatag aatctccttg gctgaacgat atgaatgcaa tccctataaa
     7141 actaattacc ccaccgatcc atccatttaa tttcattttt tcattcaaaa aaacaaaagc
     7201 aataattgcc gttactatag gtgtgactga aacaattaaa cttgcagatc cagcatttac
     7261 tgttttttcg ccgtagttta atgcaatatg ataaatagta aaccctaaag caccgaacat
     7321 aaaaattgct gggatatctt ttaaatcagg tagccgtaac ttatatataa aggagaacaa
     7381 aagaagtata aatgaagcaa ttaataaacg tagtaaagtc aaatgctcag gtgtataaga
     7441 ttcaagcccc ctacgaattg ccggaaaagc agttccccat ataagaatgg ttaaagtatg
     7501 agcacatata atgcgtaaat cccacttttt attatgcatg tttttctccc tccctaataa
     7561 accaaccatt ttcaaattac caattatttc ttttcatcat atttaatagt tgctacaatg
     7621 tcttcaacca gttatttatt gaaaaaacca accacttttc aacttttaaa taaaagaggg
     7681 agatttttca catgaaaact cataaaaaaa cgcccaaata tcagcaaatt gtagactata
     7741 taaaagaaaa aattgaaaat ggagaatggc caattggaag taagattccg agtcaacggc
     7801 agttagcaaa attatttcat gtaaatcgga gtactgtaat aactgcgcta gatgaaatta
     7861 tggcggatgg cttaattcaa ggacaaatcg gagtaggaac aattgtaacg aataatacat
     7921 ggtcattgtt agcgacgaac cctcctcccg attggagtga gtatgtaaaa gctggtatcc
     7981 ataaacccag caaattaatg gtacgagaaa taaatgaagc agaagcaaat aaaaaactta
     8041 tccagcttag taaaggcgaa ctttcacctg atcttttccc gatagaaact atgcaggcta
     8101 ttatgaagaa cgtaagcaca gaattagatt atttcggata cgaggaacaa aaagggtttt
     8161 atccattacg tgaagcaata agtacatacg taaaaacatt cggaatacat gtatcgccta
     8221 attctatatt aatcgtctct ggtgcactac aagcattaca attaatttca attggtcttt
     8281 tacatagaga gtcaactgtt ttacttgaac aaccttctta cttatattca cttcatgtat
     8341 ttcaatcagc aaatatacac ctatctggta tatcaatgga ccatcacggt attttaccta
     8401 gcgacttatt aaaacgaatt aaatacagtc aaaagcaaaa tattttatat tctattcctt
     8461 gctttcaaaa tccaacaggg atattaatgt ctaaagagcg acggaaagaa atattaaaaa
     8521 tatgcgagaa agaacagtta cctatcatcg aagacgatat ttaccgtgaa ttatggattg
     8581 atgaacaacc accagtacca ttaaaatcaa tggataaaca cggacatgta ttatatattg
     8641 gcagtctttc taaaacactt acccctggac ttagaattgg gtggattatc gggccagaac
     8701 ctgtaattga tagattatca gatataaaaa tgcaaactga ctatgggtcg agctctttat
     8761 ctcaaagagt tgctgctgaa tggataaata aaggatttta tgaggatcat gtagcaaatg
     8821 taagaattca ccttaaaaaa cgaagactaa tgatgataca agctttaaat gagtattgta
     8881 cagacattgc cacttgggac gttcctactg gcggagtttt tatatggata aagattgtac
     8941 ctaacattcc gatgaagaaa ctattttcag aggcactatc aaaaggtctt cttttaaacc
     9001 ctggacgtat ttatgaagaa aaatcagatc aatgcatacg tttatcatat ggatatgctt
     9061 cttcagaaca aatcactaac ggaattaaac tattaagcgg attaatccgt aagttaatgg
     9121 cataacaaaa actcctcatt tatctataga ggagttttta tgtgtcttat ttaccacaat
     9181 ctcatttctt gccccaataa aataagttct tcataaggat ctccgttaat ttttaatact
     9241 tgagcaaaag ctgtttcagt ttttaaacct tgttccttca agtggattat tttttctctt
     9301 atatatggat gctgcattaa cagcatgtgt tctacaatca tatgtttata tgcattttgg
     9361 ttacgcactg gcttaggttg agcaaataat tcaaattcaa aaccttcaaa tttaaaattc
     9421 acctttatcg attcggtatt tttaattttt ttctttttta ttttgaaccc ttcataagaa
     9481 ccatataaag atctcatttc ttgttcaaaa gcattaaagt gatgtacttc cattacaata
     9541 tctaagtcag attgaggagt atcaattcta atagggattg tcccgcaaag aactggacta
     9601 tataaagata aatcctccat tatgtttagt ttatttaaaa cattatatac ttgttgttgc
     9661 ttgtcattcc cagattgtaa atatgtaata gatgtaaaca ttgaagtctc tccttcattt
     9721 cacgtaatac gtgtctcact aaattcaaga aaaatttgtt ctaggagtac ctttatttca
     9781 ataaattaag ccgaactaac tatatttttc aacttttctc ctatactgca atactataaa
     9841 aataaaaagg agaaataaat atgggtattt tcaatattct tttgatcatc cacatactat
     9901 ttggcacgat ctgcttaatt accggtatta tagcgatggt tgctcaaaaa aagaaaggga
     9961 agcatacgga atggggcgaa atatatcatg catcctacgt tgttgtcaca attactgcaa
    10021 taatactatc cattatcaac tgggataaaa tcgcatattt attctatgta gcaatttttt
    10081 cttattcatt tgcgatttat ggttaccttg caagaaaaaa gcgttggaaa aattggctcc
    10141 accaccatat taggggaatg ttaggctcct atatcggtgc tgttaccgca ctattagtaa
    10201 acatcggcat tcatattccc attttaaatt tgcttccgcc aatatggttt tggtttttac
    10261 ctacctt
//
