LOCUS       NZ_NUOG01000004        10312 bp    DNA     linear   CON 31-JUL-2024
DEFINITION  Bacillus sp. AFS033286 AFS033286_82_A2_Contig7_140604, whole genome
            shotgun sequence.
ACCESSION   NZ_NUOG01000004
VERSION     NZ_NUOG01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus sp. AFS033286
  ORGANISM  Bacillus sp. AFS033286
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 93152)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 93152)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Assembly Cell v. 4.2.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 78.73x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002583065.1-RS_2024_07_31
            Annotation Date                   :: 07/31/2024 14:02:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,319
            CDSs (total)                      :: 5,247
            Genes (coding)                    :: 4,997
            CDSs (with protein)               :: 4,997
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 83 of 250
            Pseudo Genes (incomplete)         :: 197 of 250
            Pseudo Genes (internal stop)      :: 59 of 250
            Pseudo Genes (multiple problems)  :: 74 of 250
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:15:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 38340
            Orig. end                         :: 48652
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NUOG01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10312
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10312
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10312
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5312](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5312)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    122..134
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 21.78"
                     /tool="antismash"
     gene            complement(474..1103)
                     /locus_tag="COE07_RS01455"
                     /old_locus_tag="COE07_01455"
     CDS             complement(474..1103)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001994305.1"
                     /locus_tag="COE07_RS01455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_01455"
                     /product="DUF2185 domain-containing protein"
                     /protein_id="WP_098840787.1"
                     /transl_table=11
                     /translation="MSWYLDNVYELNKKSPYTFYVPSIKVFGNLKVGDLVKLIFVTKNE
                     EEDGFHGERMWVEITERNEKKFVGKLSNDPYRLNLKIGDEISFGIENICDTEYDDPASK
                     EWDFYFDTKVIVSNDVLEKREFNFMLKEDPREEGDSGWSILSGYESDDYVNDPKNFQII
                     SIGVILNIDDSILEFIRKPPLCAYERNDKGEFYKIEDYNWDAYLNG"
     gene            complement(1170..1955)
                     /locus_tag="COE07_RS01460"
                     /old_locus_tag="COE07_01460"
     CDS             complement(1170..1955)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454680.1"
                     /locus_tag="COE07_RS01460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_01460"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_098840788.1"
                     /transl_table=11
                     /translation="MKWRVQMQTKWSLSEYENPKRYDIENKSLSDFPFLLSWAQKLNIQ
                     NEWILDIACGTGRVTIPFIEYGYQMIGVDIHEGMLAEAKRKTTDCKNVKWLQQDCLQLN
                     IEETISLAFMVGHGFQHFLTNIHQNQLLTSIHHVLAENGIFIFDTRFPSKEELIQPSTE
                     EYWTTVNDEKGRKCDLYTEMTYDSIQQIQHYITTRKFYEGDTLVEELKTTIDLRYTYPQ
                     ELERLLELNGFELLHIYNDWDGKELGEDCYSMVVVCRKK"
     misc_feature    complement(1969..1985)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 24.3"
                     /tool="antismash"
     gene            2079..2816
                     /locus_tag="COE07_RS01465"
                     /old_locus_tag="COE07_01465"
     CDS             2079..2816
                     /GO_function="GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002127309.1"
                     /locus_tag="COE07_RS01465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_01465"
                     /product="ZIP family metal transporter"
                     /protein_id="WP_098840789.1"
                     /transl_table=11
                     /translation="MERLWIPMIVTFFSFGGLLLGGAVGVATRQLIEEKMHRLYALCGG
                     ILFGLLSLEIIPETFSGYEIIGPILGIAIGILVMSLLDNYFHHPMIHKIDQQAWQTFLF
                     LSFAIFIHNMPSGFALGTAFISHAESAIPFLIAIVIHHIPEGLALIIPFLFTKHKYISF
                     LLTTLLLSLILGTGTVFGILMEGKALHLQGLIMGSAIGSLGYVTIHEMLWKAKKQLSFL
                     TFLTWATSGLLLITVFTLFAGHH"
     gene            2898..4598
                     /locus_tag="COE07_RS01470"
                     /old_locus_tag="COE07_01470"
     CDS             2898..4598
                     /EC_number="1.2.3.3"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002200542.1"
                     /locus_tag="COE07_RS01470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_01470"
                     /product="pyruvate oxidase"
                     /protein_id="WP_098840790.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 1.6e-49, bitscore:
                     160.4, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.7e-42, bitscore:
                     137.4, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFEKTAGEALVDLLIDWGVEHIYGMPGDSINSIIEALRKKQDKIK
                     FIQVRHEEAGALAAAAYAKLTGKLGVCMAIAGPGAIHLLNGLYDAKLDKAPVLAISGQV
                     ESDLLGTDFFQEVNLERMFDDVAVYNQRIMSAEQLPAVVNQAIRMAYTKKGVSVLTIPD
                     DVPKFEVKNGARVTNTSFTLPELFPQKEDLSAAKLAINEATKPVILAGKGAKHARESLL
                     AFAEHIGAPIVLTLPAKGIIPDEHPLCIGGLGLIGTKPSYEAMKHADTLIMVGTSYPFT
                     GFLPEKAKTIHIDTDPAQIGKRYVTDIGLAGDADKTLKWLVANVEKHEDHTFLEHHQEM
                     MKKWEEKLHNQEEDSSTPIKPQRVMHALQKVAEDDAILSVDVGNVTVWTARHFRMTNQQ
                     FIISSWLATLGCGLPGALAGKIAFPDKQVFAVCGDGGFGMTMNDFVTAVKYKLPIVVVV
                     LNNNKIAMIKFEQEVMGNIEFGTDLTNPDFAKYAEACGGIGYRVENLDELLPAFESAVK
                     QNRPCIIDVVVDVNEAPMPAKITFGQAAGYTKHMIKELFEEGKIDLPPL"
     gene            complement(4644..>4781)
                     /locus_tag="COE07_RS28150"
                     /pseudo=""
     CDS             complement(4644..>4781)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267869.1"
                     /locus_tag="COE07_RS28150"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="peptide ABC transporter permease"
                     /pseudo=""
                     /transl_table=11
                     /translation="ISNSMQEAVKLGNVKRPKRENKEVKVDKKIQSNVNNISFYREVQK
                     "
     gene            complement(5001..5312)
                     /locus_tag="COE07_RS01475"
                     /old_locus_tag="COE07_01475"
     CDS             complement(5001..5312)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071369.1"
                     /locus_tag="COE07_RS01475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_01475"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_176550862.1"
                     /sec_met_domain="TIGR03601 (E-value: 4.8e-38, bitscore:
                     122.2, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKFQQELQALSLNDYHSGNIVYWDQQNQYPYYYIEDAARRCGGC
                     GRCGGGGFRCGGFRCFGCFGCFSCGGCGGCGGCGGCGGCSNCFNGFTDTTGTIITYEY"
     gene            complement(5637..6638)
                     /locus_tag="COE07_RS01480"
                     /old_locus_tag="COE07_01480"
     CDS             complement(5637..6638)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1085:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267869.1"
                     /locus_tag="COE07_RS01480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_01480"
                     /product="ABC transporter permease"
                     /protein_id="WP_088233350.1"
                     /transl_table=11
                     /translation="MWQVVKRDVRFWIGVTFLSVLMIVSIGNTVFFDGNIREITMMYNE
                     KGELEAAPFSPSSKFWFGTDVKGRDLFQLIIEGAKWTVGASIVIAVLRVVIGGAIGLLL
                     GMYSKRSFPVISSFFDPFSIVPMIMISYFILKEVLMFDSGEAAAPFHLRVAFQILVLTC
                     LAVPTVMLYVAQEVKRIKKEEFMLAATVLGGSKWQRLKHHVWPHMLPSFLLLVAQQFVS
                     VLLLLMHLGLLKLFFGGTIMFGLDPDSVTKEWTGLIGQNFRHLTTHTWIVLIPIAFYSM
                     TILSGNLISNSMQDAIKLGNVRMPKKKDKEVKVEKQSQPTVNGFSFHREVSK"
     gene            complement(6640..7506)
                     /locus_tag="COE07_RS01485"
                     /old_locus_tag="COE07_01485"
     CDS             complement(6640..7506)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1118:
                     binding-protein-dependent transport systems"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002148061.1"
                     /locus_tag="COE07_RS01485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_01485"
                     /product="ABC transporter permease subunit"
                     /protein_id="WP_098253440.1"
                     /transl_table=11
                     /translation="MFIRIGKWCGVTCLQLFAAFICILFLGALPRLFKGLQIDLIGFWN
                     TVVFLGEKLLQPGEITYGFRNSRKLFPQIWIHYVETMFVFLSAFILSLLIAYFIVVWVL
                     QRSQSKQRIWNGVFVALESIPDILLILLSQLLVVIIFQKTGFMPVKHAGLGEEKVRMLP
                     IICLAIPTTLLFIKLLLLRFREELEKDYSIFAKSKGLSLRYILTHHISRNVLLTTVYYA
                     KTNILFMLSNLYIIEWLFNTYGMFVFVKENSKLEIFTVSLVILYVPLFILFRILHTLLQ
                     NVIKERV"
     gene            complement(7728..9611)
                     /locus_tag="COE07_RS01490"
                     /old_locus_tag="COE07_01490"
     CDS             complement(7728..9611)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1243: ABC
                     transporter, permease component"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002031895.1"
                     /locus_tag="COE07_RS01490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="COE07_01490"
                     /product="FtsX-like permease family protein"
                     /protein_id="WP_088233351.1"
                     /transl_table=11
                     /translation="MLFKLSRHSMKKMLKDYMVLLIGLVISISIFYMFQTMAMNSEFTK
                     DNSLISSIRLVFWVGAILLSFITVFYIIYANSFLLTLRRKELGMYMMLGAKKKKVAQLL
                     FIETFGMGIVSIIIGILVGMLLASVAGNFLINGMEISAKGSYSSVYTPAIIVTAIFFLI
                     LFFITGLMNSIRLLRKTELELIREDETLDEVKKSKTRIIIMTVFGMLLVSTGYYFMVNI
                     KLFAEVGFIIATIVTTLGTYFIFSSLLPFFVMKIKGNKKRNETGLNSFTYAQLRFRVNS
                     LSRVLGTVAMLIALGAGAMTAGMAFQKNVGIMTDFSRVYDVVIHDPNEQDRAALKEMEI
                     VEESKYKYKVDGEAVYYLRNDLTAKPPLVSDHFDTKTLKEPARKRVAEPLTEPVYSALD
                     APEAANKLPRMPKDWEDAVVREIQITHNQFNGKPVRIADEEHYKAVQGTEHSVILVRVD
                     DMKKYKPLLLDIDKRQKELIEKTIGAKVELYTKMTIYQFMNSFMSGTMFMGFFLGIAFL
                     AMMASSLMFKILTGASRDVRRYEMLRKIGVRRSMLTKSIYKEISYLFIFPAIIGISHVL
                     VGLNLFSFILVDPFVKVWVPIGIFLVIYFIYYWITVQLYKGMVMPKEEVAK"
     misc_feature    9569..9584
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 23.21"
                     /tool="antismash"
ORIGIN
        1 caatccgcac aaaattcaat ttcagtaccc tcttttaccc gtgcgtccaa cgcgtcatct
       61 gtccattcat aagatactag tttctgcatc tcttttttcg tttcagtatg cgcaatagtt
      121 aatgcatctt gcaatacagg caatccaaaa atatgatccc agtgccaatg tgttaatact
      181 acaaagtcag gacttgttac attctgttct tttaacattt ctaaaaataa ttgtgcatgt
      241 gcttctgaat tccccgcatc aatcattaaa gttttttctt ttccaacgac cattcctaat
      301 ataggtctat ccgtttcaga aacaggtgtc atataccaaa atgagtttcc tatttttttt
      361 atttgctgca taattagtac ctccatttac tactactctc ttcaattttt atttgtacta
      421 gcgcatagcg tgaaataata tatattgctt tctttaaaac gtagtaactt cttctatcca
      481 tttaaataag catcccaatt ataatcttct atcttataaa attcaccttt atcatttctt
      541 tcatatgcac ataaaggtgg tttcctaata aattccaaaa tagaatcatc tatattcaat
      601 atgactccaa ttgaaataat ttgaaagttt ttgggatcat ttacataatc atcactttca
      661 tagccactta atattgacca accagagtca ccttcttctc ttggatcctc tttaagcatg
      721 aagttaaatt ctcttttttc aagtacatca ttactaacaa ttaccttcgt gtcaaaataa
      781 aaatcccact cttttgaagc tggatcgtca tattccgtat cacatatatt ttcaattcca
      841 aatgaaattt catcacctat ttttaaattt aaacgatacg gatcattgct cagtttccct
      901 acaaacttct tttcatttct ttcagtgatt tcaacccaca ttctttcacc atgaaaccca
      961 tcttcttctt cgtttttagt tacaaaaatt aactttacca aatctccaac ctttaaattc
     1021 ccaaaaactt ttatactggg aacataaaat gtatatgggg attttttatt taactcataa
     1081 acgttatcta aataccagga cattttttca ccctcctaaa ataataatta tgtaagacac
     1141 caattcaatc cttagagatt tttatactat tatttcttcc gacaaacaac caccattgaa
     1201 taacaatcct ctcctaactc cttcccgtcc caatcattat atatatgtag caactcgaac
     1261 ccgttcaact ctaacaatct ttctaattct tgcggatacg tataacgaag atcaatcgtt
     1321 gtttttagtt cctctactag cgtatcaccc tcataaaact ttcttgttgt tatgtagtgc
     1381 tgtatttgct gaattgaatc gtatgtcatt tcagtatata aatcacattt cctacccttt
     1441 tcatcattaa cagttgtcca atattcttct gtagatggtt gtattaattc ttcttttgaa
     1501 gggaaacgtg tatcgaatat gaatatacca ttctcagcta acacatgatg aatagatgtt
     1561 aataattgat tttgatgaat attcgtaaga aaatgctgga atccatgccc caccataaat
     1621 gctagcgaaa tggtctcttc aatattcaat tgtaagcagt cttgttgtaa ccattttaca
     1681 ttcttacaat ccgtagtttt tctcttcgct tcagcaagca ttccttcatg tatatctaca
     1741 ccgatcattt gatatccata ttctataaat ggaattgtaa ctcttcctgt tccacaagca
     1801 atatctagaa tccattcatt ttgtatattt agtttttgtg cccatgatag taagaacgga
     1861 aaatctgaca aacttttatt ttcaatatca tatcgttttg gattttcgta ttcagataaa
     1921 ctccattttg tttgcatttg tactctccat ttcatttcaa tatataaatt ataataaatt
     1981 tacagttttt tctctataat tagtcataat aatttggact aatctaactt tccttctcgt
     2041 aagctaaaaa taagggtata gaatcggagt gtgatactat ggaacgatta tggattccaa
     2101 tgattgttac gtttttttca ttcgggggat tactattagg aggggcagtt ggagtagcaa
     2161 cacgccaact aattgaagaa aaaatgcatc gtttatacgc attatgtggt gggattttgt
     2221 ttggactttt atcacttgaa attattcctg aaacattttc aggctatgaa ataattggtc
     2281 ctatacttgg tatagctatc ggtattttag ttatgagctt attagataac tattttcatc
     2341 atccaatgat acataaaata gatcaacaag catggcaaac tttccttttt ctctctttcg
     2401 ctatattcat tcacaatatg ccaagtggat tcgccttagg cacagctttt attagtcatg
     2461 ccgaatctgc cattcccttt ttaatagcaa ttgttattca tcatattcca gaaggattag
     2521 ctttaataat cccctttctc tttacaaagc ataaatatat ttccttttta ttaacaactt
     2581 tattactctc tcttattctc ggtaccggta cggtttttgg cattttgatg gaaggaaaag
     2641 ctcttcacct gcaagggctc attatgggaa gtgctatcgg ttcacttggc tatgtcacaa
     2701 ttcatgaaat gctttggaaa gctaaaaaac agctctcttt ccttacgttt ttaacatggg
     2761 caactagtgg tttattacta ataacagtat ttacactatt cgctggtcat cattaaagct
     2821 aaaatttttt tcatttgtat atttttcctt tttctccaaa acataatgtt atctacctat
     2881 tataaggagg aaccattatg tttgagaaaa cagctggtga agcattagtt gatttattaa
     2941 ttgactgggg agtagaacac atatacggta tgcctgggga ttccatcaat tctataattg
     3001 aagctttaag aaaaaaacaa gacaaaatta aattcatcca agttcgccac gaagaagctg
     3061 gcgcattagc agcggctgcc tatgcgaaat taacaggaaa actaggcgta tgtatggcta
     3121 ttgcaggacc aggtgcaatc catttactca atggtttata tgatgccaaa ctcgataaag
     3181 cccctgtact agcaatttca ggacaagtag aaagcgattt gctcggaaca gattttttcc
     3241 aagaagtaaa cttggaaaga atgtttgatg atgttgcggt ttataaccag cgcattatgt
     3301 cagcagaaca gttacctgct gtagtgaacc aagccattcg aatggcttat acgaaaaaag
     3361 gtgtgtccgt tcttactatt ccagatgatg tgccgaaatt tgaagtgaaa aatggtgctc
     3421 gtgttacaaa cacttctttt acattgcctg agctatttcc tcaaaaagaa gatttatctg
     3481 ccgcgaaact cgcaataaat gaagcgacaa agcctgttat tttagctgga aaaggcgcaa
     3541 aacatgcgag agagtcttta cttgcatttg cagaacacat tggcgctcca atcgttctta
     3601 ccttaccagc taaaggaatt attcctgatg aacaccctct ttgtatcggt ggattaggat
     3661 taattggaac gaaaccttct tatgaagcaa tgaaacatgc ggatacttta attatggttg
     3721 gtacttctta tccatttact ggtttcttac ccgaaaaggc aaaaacaatt catatcgata
     3781 cagatcctgc ccaaatcgga aagcgttatg tgacagatat cggcttagct ggtgatgctg
     3841 ataaaacatt aaaatggtta gttgcaaatg ttgaaaaaca tgaagaccat actttcttag
     3901 aacaccatca agaaatgatg aaaaagtggg aagaaaaatt acacaatcaa gaagaagact
     3961 catccactcc aattaaacca cagcgagtta tgcacgctct gcagaaagta gcagaagatg
     4021 acgcaattct ttcagtcgat gttggcaatg taacagtatg gactgcaaga catttccgta
     4081 tgacaaacca acaatttatc atttctagct ggcttgcaac acttggttgt ggtttacctg
     4141 gtgccctcgc tgggaaaatt gcgttcccgg ataaacaagt gtttgcagtt tgtggtgatg
     4201 gtgggtttgg aatgacaatg aacgactttg taacagctgt taaatataag ctaccaatcg
     4261 ttgttgtcgt attgaacaac aataaaattg ctatgattaa attcgaacaa gaggttatgg
     4321 ggaatataga attcggtaca gacttaacaa accctgattt tgcaaaatac gcagaagctt
     4381 gtggtggtat tggataccgt gttgagaatt tagatgaact acttcctgct ttcgaaagtg
     4441 cagtaaaaca aaacagaccg tgtattattg atgtagttgt agatgtaaat gaagcaccaa
     4501 tgccagcaaa aatcacattt ggtcaagcag ctggttatac gaaacatatg ataaaagaat
     4561 tatttgaaga aggtaaaatt gatttacctc ccctttaaac aaaataagat agctgaaaac
     4621 aaattgtctt cagctatctt attttatttt tgtacctctc tataaaaaga aatattattc
     4681 acattagact gtattttctt atctactttc acttctttat tttccctctt cggccttttc
     4741 acatttccta gcttaactgc ctcttgcata ctattactaa tatcacgaat gatttttgag
     4801 tattcacatt aatattatct acttaattaa gtcttttttt caagtgacat atcagcgata
     4861 ttttatatct atttcgtata tgtgttagca agctcgtttg caaacttgtt agcgcttttt
     4921 tataaaattt gtataatgct ttaaaaagta tgaatccgtt ttaatcaatc tttttcaaaa
     4981 tggattcctt ttgtctctta tcaatattca tatgttataa tagtaccagt agtatcagta
     5041 aaaccattaa aacaattaga gcaaccacca caaccaccac aaccaccaca accgccacag
     5101 ccgccacaac taaagcaacc gaagcaacca aaacaacgga atccaccaca acgaaatcca
     5161 ccaccaccac aacgaccaca accgccacag cgacgagcag catcttctat atagtaatat
     5221 ggatattgat tttgctggtc ccaatacaca atatttccag aatggtaatc attcaggctt
     5281 aacgcttgta gttcttgttg aaacttattc attttcataa cctccattta ttaactacga
     5341 ccccatagat gctttcctat atttctgtct gttaaatcta agtagaaata gcgctataaa
     5401 ctaagttcaa tacgtacacc aacaaaatat gacctatcac tagatgatgc accttgttca
     5461 tatacccatt tttagttttt ttactatggg ttcattttta taaatggaaa ttataatttc
     5521 cctggtgcac ttgctggaaa aattgctttt ccgtataaac aagtatttgc agttttacct
     5581 cccctttaaa atataaaaaa ggtagctgaa aacttattat tttcagctac cttcctctat
     5641 tttgatacct ctctatgaaa tgaaaaaccg ttcacggtag gctgcgattg tttctctact
     5701 ttcacttctt tatccttctt ctttggcatt cttacgtttc ctagttttat cgcatcttgc
     5761 atactattac taatcagatt tcccgacaat attgtcatac tatagaaagc gattggtatt
     5821 aatacaatcc atgtgtgtgt agttaagtga cggaagtttt ggccaattaa tcctgtccat
     5881 tcttttgtta cactatctgg atctaatcca aacattatcg ttcctccgaa aaacaactta
     5941 agtaaaccaa gatgcataag aagtaatagc acacttacaa attgttgagc tactaataaa
     6001 agaaaagatg gtaacatatg tggccataca tggtgcttca gtctctgcca cttacttcct
     6061 ccaagcactg ttgccgccaa cataaattct tctttcttaa tacgcttcac ttcttgcgct
     6121 acatacaaca taactgtcgg tactgcaaga catgtaagaa ctaaaatttg aaatgcaact
     6181 cgcaaatgaa acggagcagc tgcctcccca ctgtcgaaca ttaaaacttc ctttaatata
     6241 aaataggaaa tcataatcat cggtacaata ctaaagggat caaaaaagga tgaaataact
     6301 ggaaatgagc gtttactgta cataccaagt aataagccaa tagctccgcc aattactaca
     6361 cgtaaaactg cgataacgat actcgctcct acggtccatt ttgccccttc tattattaat
     6421 tgaaaaagat cgcgtccttt cacatcagtt ccgaaccaaa atttactaga tggtgaaaat
     6481 ggagcagcct ctaattctcc cttctcgtta tacatcatcg ttatttcacg aatattccca
     6541 tcaaaaaaca ccgtatttcc aatactaaca atcattaata cactcaaaaa agtaacacct
     6601 atccaaaatc taacatccct ttttacaact tgccacatgt tacacacgct cctttataac
     6661 attttgcagc agcgtatgta atatacgaaa tagtataaaa agcggtacgt ataatataac
     6721 taaactcact gtaaatattt ctaatttaga attttctttt acaaaaacaa acatgccgta
     6781 cgtattaaat agccattcaa taatatataa atttgataac ataaataaaa tatttgtttt
     6841 tgcataatag actgttgtta acaagacatt tcttgaaata tgatgcgtca aaatatatct
     6901 taaacttaat cctttactct tggcaaatat actataatct ttctctaatt cttctctaaa
     6961 acgtaataat agtagcttaa taaataataa tgttgtaggt atagcaaggc atattattgg
     7021 taacatgcgc actttttcct cacccaatcc cgcatgtttc accggcataa atcctgtttt
     7081 ttgaaagata attacaacta gaagctgaga tagtaaaatt aataaaatat ctggaatact
     7141 ttcaagcgct acaaaaactc cattccatat tcgttgcttt gattgcgagc gttgtaatac
     7201 ccacacgacg ataaaatatg caataagtaa agatagtata aatgcggaaa ggaacacaaa
     7261 cattgtctct acataatgaa tccatatttg tggaaataac tttcttgaat tccgaaatcc
     7321 ataagtaatt tcacccggtt gaagtaactt ctctccaaga aacacaactg tgttccaaaa
     7381 accgattaaa tcaatttgca gtcctttaaa taaacgtggt agcgccccta aaaaaagaat
     7441 acaaataaat gcggcaaaca attgtaaaca tgtaacccca caccacttac caattcgaat
     7501 aaacataaca attacccctt ttatttaaat gaatattcag aaataaatac catgtattgt
     7561 gcctatacct tgtaatgaaa tcacccctct ctcgacaaaa cgttgcgatt cccgtcattt
     7621 ctcggagctg tattatattt ttcgactttt atccaacatt ttccttctca attcattaca
     7681 tactagaaaa gagcccttgt tttcaaaaca agggcttttt tcttaaacta ttttgctacc
     7741 tcttctttcg gcattaccat acctttatac aattgaaccg ttatccagta ataaataaag
     7801 taaatgacca agaagatccc tatcggcacc catactttca caaatgggtc aactaatata
     7861 aagctgaata agttaagccc tactaacacg tgggaaatac caataatcgc tgggaagata
     7921 aataaatatg aaatttcttt atatatactt ttcgttaaca tactgcgtct cacaccgatt
     7981 tttcgtaaca tttcatagcg gcgtacgtca cgtgaagcac ctgttaatat tttgaacata
     8041 agggaacttg ccatcattgc taaaaaggca attccgagga aaaatcccat aaacattgtt
     8101 ccgctcatga aactgttcat gaactgataa attgtcattt tcgtatatag ttctactttt
     8161 gcacctattg ttttttcaat taattctttt tgtctcttat ctatgtcgag caacaatggt
     8221 ttatattttt tcatatcgtc tactcttact agtattacac tatgttctgt tccttgaact
     8281 gccttataat gctcctcatc agcaattctt acaggttttc cgttaaattg attatgtgta
     8341 atttgtattt ctcttacaac tgcatcttcc caatcttttg gcatgcgagg aagcttattc
     8401 gctgcttcgg gagcgtccaa agcagaatag acaggttcag ttaaaggttc agccacacgt
     8461 ttacgtgcag gctcttttaa agtttttgta tcaaaatgat cggaaacaag tggcggtttt
     8521 gcagttaagt cgttacgtaa gtaatacacc gcttctccat ctactttata cttatacttg
     8581 ctttcttcaa caatttccat ttcttttaat gctgctctat cttgttcatt tggatcatga
     8641 atgacaacat cataaacacg tgagaagtct gtcataatac ctacattttt ttgaaatgcc
     8701 atacctgctg tcattgcacc tgcacctaat gcaattagca tcgctacagt gcctaatact
     8761 ctcgataaac tatttactcg aaagcgtagc tgtgcatatg taaaactatt taagcccgtt
     8821 tcatttcgtt ttttatttcc tttaattttc atcacaaaaa atgggagcaa tgaactgaaa
     8881 ataaaatacg ttccaagtgt cgtaacgatt gtcgctatta taaaaccgac ttccgcaaat
     8941 aattttatat tcaccataaa atagtaccct gtacttacta gtaacatacc aaatactgtc
     9001 attataataa tgcgagtttt acttttcttc acttcatcta atgtttcatc ttcacgaatt
     9061 aactctagtt ctgttttacg taataaacgg atgctattca ttaaaccagt aataaaaaat
     9121 agtattaaga agaatatagc tgtaactata atcgctggtg tatatacaga tgaataacta
     9181 ccttttgctg aaatttccat gccatttatt aaaaagtttc ctgctacact tgctagtaac
     9241 attcctacta aaataccgat aataatacta acaataccca taccgaatgt ttcaataaat
     9301 aatagctgtg ctactttttt cttttttgct ccaagcatca tgtacatacc tagttctttt
     9361 cttcttaatg tgagcaagaa agagttggca tatataatat aaaataccgt aatgaaagat
     9421 aatagtattg caccaaccca gaagacgagt ctaatactac taatcagact attatctttc
     9481 gtaaattcac tattcatcgc catcgtttgg aacatataaa aaatactaat actaataacg
     9541 agaccaatca gtaaaaccat atagtctttt aacatctttt tcatactatg acgtgataat
     9601 ttgaataaca tagtctcgcc cccctactta gactcagaag ctgagcccat atgagcgagc
     9661 acacctaaaa tgtcttgata gaaattttct cgtgttcctt gacgatgtaa ctctttatat
     9721 agaagaccgt cttgaataaa taaaatacgt tcacaataac tcgcactaaa agcatcgtgt
     9781 gtaaccatta aaatacttac attgtgattt tcatgtaaat cttgcatcgc ttctaataaa
     9841 ctttttgcgt ttttactatc aagcgctcct gtcggttcat ctgctaatac gattgctgga
     9901 ttatgtacta aagctcgtgc tgccgctgtt ctttgctttt gaccgccaga aacagcggac
     9961 ggatatttcg ttaaaatttc agtaatccct aatttatttg caacttcatt gacctttcca
    10021 gtaatctcac ttgatggtac tccttgcaaa gaaagtggta aagcgatgtt ttcataaatt
    10081 gataaatttt ctagtaagtt aaaatcttga aaaatgaatc ctaatttttg agatcgaaaa
    10141 tctgataatg cattaccttt catagaagta atatttatgc cagctatcgt cacactacca
    10201 ccagtcgctt gatcaagtgt actaatcaca ttaagtaatg tcgtttttcc agatccagaa
    10261 ggacccataa tccctacaaa ctccccttct ttaatactta atgaaacacc cc
//
