LOCUS       NZ_NUIO01000070        10330 bp    DNA     linear   CON 20-MAY-2024
DEFINITION  Bacillus thuringiensis strain AFS049207
            AFS049207_100_C7_Contig31_140514, whole genome shotgun sequence.
ACCESSION   NZ_NUIO01000070
VERSION     NZ_NUIO01000070.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 137818)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 137818)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Assembly Cell v. 4.2.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.45x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002575705.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 01:16:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,769
            CDSs (total)                      :: 5,694
            Genes (coding)                    :: 5,513
            CDSs (with protein)               :: 5,513
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 72 of 182
            Pseudo Genes (incomplete)         :: 113 of 182
            Pseudo Genes (internal stop)      :: 64 of 182
            Pseudo Genes (multiple problems)  :: 54 of 182
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:44:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 91186
            Orig. end                         :: 101516
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NUIO01000070.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10330
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10330
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10330
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5330](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5330
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    433..447
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 17.35"
                     /tool="antismash"
     gene            523..1971
                     /locus_tag="CN977_RS24835"
                     /old_locus_tag="CN977_24835"
     CDS             523..1971
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-469). E-value:
                     2.9e-30. Score: 97.5. Matches aSDomain:
                     nrpspksdomains_CN977_RS24835_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="CN977_RS24835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_24835"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_000471636.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 7.6e-30, bitscore:
                     97.4, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYLKKMIMKGHLLGNQKIPSTRELSEL
                     LSVSRNTVLAAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKNKLNTVTALADE
                     LDLMKHGVRWEKGMIVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYKPL
                     MNYLLHYMEMKGVDISNKDILITNGFTEGLDIVLSSLSKKSGRVICENPTHHAALKLFR
                     LHGLEIHGIDMNEDGIDTNEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYKVPIIEDGFNEELRYSGSHLAPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQF
                     IPFKRMTGDGGLHLFIELEEHMNARTLLQKCYEKGVTFSPGDVFYSDGEGANTFRLGFS
                     RLKEEEIVRGIKIIGDTLKNEIWS"
     aSDomain        958..1929
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CN977_RS24835_Aminotran_1_2.1"
                     /evalue="2.90E-30"
                     /label="CN977_RS24835_Aminotran_1_2.1"
                     /locus_tag="CN977_RS24835"
                     /protein_end="469"
                     /protein_start="145"
                     /score="97.5"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYKPLMNYLLHYMEMKGVDISNKDILITNGFT
                     EGLDIVLSSLSKKSGRVICENPTHHAALKLFRLHGLEIHGIDMNEDGIDTNEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYKVPIIEDGFNEELRYSGSHLAPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQFIPFKRMTGDGGLHLFIELEEHMNARTL
                     LQKCYEKGVTFSPGDVFYSDGEGANTFRLGFSRLKEEEIVRGI"
     gene            1976..2890
                     /locus_tag="CN977_RS24840"
                     /old_locus_tag="CN977_24840"
     CDS             1976..2890
                     /EC_number="6.3.2.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="CN977_RS24840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_24840"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_016077645.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 2.3e-23,
                     bitscore: 76.0, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 7.4e-15, bitscore:
                     48.6, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 1e-09, bitscore: 31.4,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 1.5e-54,
                     bitscore: 177.8, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 2.8e-07, bitscore:
                     23.5, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRY
                     EGVETPDWIELTKMEDLKLDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVF
                     EWDSEVVIEKYVKGDEITCSILDGKQLPIVSIRHAAEFFDYNAKYDDTSTVEEVIELPA
                     EINERVNKASLACYKALKCSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAG
                     ISYSKLLDMIIETSLKVRKEEGF"
     misc_feature    complement(2913..2925)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     regulatory      2964..3069
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            3197..3886
                     /gene="tenA"
                     /locus_tag="CN977_RS24845"
                     /old_locus_tag="CN977_24845"
     CDS             3197..3886
                     /EC_number="3.5.99.2"
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="CN977_RS24845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_24845"
                     /product="thiaminase II"
                     /protein_id="WP_074790141.1"
                     /transl_table=11
                     /translation="MTFSQSLRKEVDSIWEASFNHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITKEEKDNFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWISAY
                     GSDWFRTLVEEQIIRLDTIAEKVTEADRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDT
                     DVKDVIG"
     gene            4284..4547
                     /locus_tag="CN977_RS24850"
                     /old_locus_tag="CN977_24850"
     CDS             4284..4547
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101034.1"
                     /locus_tag="CN977_RS24850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_24850"
                     /product="DUF3937 domain-containing protein"
                     /protein_id="WP_002082716.1"
                     /transl_table=11
                     /translation="MNFERCYMFTNKKLIRFGLSLFVFLGIINFTISYFQTYLETAADI
                     KWVIPEIWKTILLDVPQGILVLLGAIALYDFTKEASQKDASI"
     gene            5001..5330
                     /locus_tag="CN977_RS24855"
                     /old_locus_tag="CN977_24855"
     CDS             5001..5330
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071363.1"
                     /locus_tag="CN977_RS24855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_24855"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001071349.1"
                     /sec_met_domain="TIGR03601 (E-value: 6.5e-40, bitscore:
                     128.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQEIQALSLNDYRSGNIVYWDQQNQYPYYYIEDAARRCGGC
                     GRCGGCGGGRCGGFRCGGFRCIGCFGCFGCGGCGGCGGCGGCSNCFDGFSGTTDTTGTI
                     ITYEY"
     gene            <5480..5614
                     /locus_tag="CN977_RS30085"
                     /pseudo=""
     CDS             <5480..5614
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016077325.1"
                     /locus_tag="CN977_RS30085"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="site-specific integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="YMYVPTKNIVIINGYIQSFK"
     misc_feature    complement(5774..5792)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.87"
                     /tool="antismash"
     gene            5824..6309
                     /locus_tag="CN977_RS24860"
                     /old_locus_tag="CN977_24860"
     CDS             5824..6309
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124614.1"
                     /locus_tag="CN977_RS24860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_24860"
                     /product="hypothetical protein"
                     /protein_id="WP_002082715.1"
                     /transl_table=11
                     /translation="MNMVELKSIFHSYKVKRKIAKDLYGKRDELTLLLNEFNNMKCTVT
                     SEKKKNNILSRLQLIYQNIKLDKQYPLSVSFDSKLLERLEKESLHTIEDGVTCLHLMLD
                     MNYEKIKQYGSSTSRSFVPLSQSSICLADCICLTGFIVGLLGVISFGGFMLSICSTT"
     misc_feature    complement(6556..6569)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.22"
                     /tool="antismash"
     misc_feature    6611..6624
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.3"
                     /tool="antismash"
     gene            6616..7317
                     /locus_tag="CN977_RS24865"
                     /old_locus_tag="CN977_24865"
     CDS             6616..7317
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736205.1"
                     /locus_tag="CN977_RS24865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_24865"
                     /product="DUF3962 domain-containing protein"
                     /protein_id="WP_086396620.1"
                     /transl_table=11
                     /translation="MKKLRLLTFENIVEPLLNEKVSFIYFPIEWLDIVEIHYKTFLLTS
                     KLKRLNERLYDMFSDILFIQHNPYVLNENTPWIVSKEPIKQEQLNYIFQSWYEIIHDWK
                     PNKLVEPPKYEWQSDLISNLPVLHDNETYSKWVPALISHIFCERPIYLENTNAEEIYFS
                     PLRSQNICEAMSGPIKDEKTQDFFSYVYRFECITRGGENAPLLNISIGIRRFYQEYKMI
                     GQTNPDMTTFV"
     gene            complement(7356..8465)
                     /locus_tag="CN977_RS24870"
                     /old_locus_tag="CN977_24870"
     CDS             complement(7356..8465)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1077: phage integrase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675870.1"
                     /locus_tag="CN977_RS24870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_24870"
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_098829710.1"
                     /transl_table=11
                     /translation="MKGHIRKRGNKYCIVIDIGPDPETGKRRQKWFSGYKTKKEAQADV
                     AKKITELNEGTFIEPSKVTLKDYLNHWLEIKSISIQKSTFAGYKAFINQHVIPSIGMVA
                     LHKLNVMHIQKCYKTAIDKGITNNSILLMHRILKSALNLAVKQNILSRNPADFAEIPKK
                     EKTPIQTWTEEEVKKFLAHSQESRYHIGYLLAITTGMRLGEVLGLRWQDIDFEKHTVTI
                     NQTSGHDNKIKKTAKTNSSKRTIPVPNETIVALKKHKILINKEKLRFGSAYLDQDLINC
                     NEFGRIIKRAHFRKSFIRATHKVGIKEIKFHDLRHTHATILLKQGVNPKIISERLGHTD
                     ISMTLSVYSHVLPNMQEEAVKNFGKSIFG"
     gene            9013..10164
                     /locus_tag="CN977_RS24875"
                     /old_locus_tag="CN977_24875"
     CDS             9013..10164
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265310.1"
                     /locus_tag="CN977_RS24875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_24875"
                     /product="AimR family lysis-lysogeny pheromone receptor"
                     /protein_id="WP_098829711.1"
                     /transl_table=11
                     /translation="MWKALNQIEKELCAAGIRKNKLANYWGVKPSTVTKVFKGNTDMSF
                     GFLSKTVILLNKGIQVQENLLTDYINITKPKSENLREAMEDLALRGKFNLLINIINSES
                     QSKVAENREFANVYRIIYRRYIGEIDAIQYHKALSFESKSIRTSEMEVLIEILLCQAQY
                     QSGNFTSLNERLKSLEIKLNKISNRYIRECYKLRYKEAIAVTSLQGGEVIEARHVSMEL
                     LDDLEWDNFFSFPKVNAYLKLGESYIFSANEYEKAKYYLEKTLEVLGDSKINGIERKRK
                     MVQHTLSFLKIHHDKEISSIDVVHPGELAYLRIKQGKRIEARKLLIQLKEKNGRLTDIQ
                     TAYLGLTYEGTKKEELMKRSLLMCQKSGNIFYSNLPKIHLGLI"
ORIGIN
        1 gaaagaagcg caaaataaag gaattgctga gacaaaccct atattagata taagtggttc
       61 agatagtgcg tgtaaattgt tacttttgac aaacagctta atggaaacag agaaaacact
      121 tactgatata catataaaag gaatcgagca cgttacaaaa cagcaaatac aaaatgctaa
      181 ggaacaacat aaaattatta aattaatagc atcaatatat aaggatgagg gtggcaatgt
      241 gaatttaaat gttgaaccgt gcgaaataga aaaagatcat ccgttagcaa aagtaaatgg
      301 gaccgaaaaa ggaattacgt tctttacaga tacgatggga caagttacta caattggtgg
      361 ggcttctaat ccacgaggcg ctgcagctgc agctttaaaa gatgtaatta acttatatcg
      421 aaaagacttg taagaccgta ccgcccttta taaaaaatac atatgtcgtg gaccttttgt
      481 gaatgaaata aatatgcaaa aatgattgca gggggtaagg aattgtttaa ggattttaaa
      541 gttgttaaag atcgtcccgt ttatattcaa ttgaaagatt atttgaagaa gatgattatg
      601 aaagggcact tgctcgggaa tcaaaaaatc ccatcaacaa gggaactaag tgaattacta
      661 agtgtgagta gaaacactgt acttgctgct tatgcagatt tagagcaaga aggactcatt
      721 tatgcagtta aaggaaaagg gaactttgtt gcgaaggtag atatatctaa cacttcgtct
      781 gttgaaatag attggaaaaa taaacttaat acagtgaccg cattagcgga tgaattagat
      841 ttaatgaaac atggtgttcg ttgggaaaag ggaatgattg tttttaatag tatagccccg
      901 gatgagaagc tatttgatgt tgaaaacttc aaaagagctt ttcttactcg tatgtccatt
      961 gaaggggaca tcgtattgaa ctacggatat gcaaagggat ataaaccgtt aatgaattat
     1021 ttacttcatt acatggaaat gaaaggtgta gacatttcga ataaagatat tctaattaca
     1081 aatggattta cagaaggatt ggatattgta ctttcttctt tatcaaaaaa atccggtcgt
     1141 gtcatttgtg agaatccaac tcaccacgct gcactaaagc tgttccgttt acatggactt
     1201 gaaattcatg ggattgatat gaatgaggat ggtattgata cgaatgaagt tgaaaaaagc
     1261 ttacgtgaaa aagattttga ttttgcttat ttaattcctt cttatcataa tccaaccggt
     1321 attgttacga gttcagagaa aagaacggaa ttgatgaggt tattctcgaa atataaggtt
     1381 cctattatag aggacggatt taatgaagaa ttacgttatt caggttcaca tttagctccc
     1441 ttattaactt ttgctggagc tggcaataat gtgatttata ttagtagctt ttcaaaggta
     1501 cttttccctg gtttacgtgt aggttggatt attgcagata aagagctgat tcattattta
     1561 gaaagcgtaa aaagagcaag aacaatccac acatctacgt tagatcaagc ggttctattt
     1621 caatatttac atgagggata ttttgagaaa tatttaaaaa aggcaagatc ggtttataag
     1681 aaaaaatatg agttagctgt cggcgcgtgt aatcagttca ttcctttcaa aagaatgact
     1741 ggagatggtg gacttcattt atttatagag ctagaagagc atatgaatgc ccgcacactt
     1801 ttacaaaagt gttatgagaa aggcgttacg ttttcacctg gagatgtttt ttactctgat
     1861 ggagaagggg cgaacacttt ccgattaggg ttttcgcgct tgaaagaaga agaaatagtt
     1921 cggggaatta aaataattgg tgatacatta aaaaatgaaa tttggagttg agaaaatgaa
     1981 aatcggtgtt attatgggcg gggtatcgtc tgaaaaacaa gtttcaatta tgacagggaa
     2041 tgaaatgatt gctcatttag ataaaaataa gtatgaaata gtcccaatta cattaaatga
     2101 aaaaatggat ttaattgaaa aagcgaaaga cattgacttt gcgttgctcg cattacacgg
     2161 gaaatatgga gaagatggga cagttcaagg aacactggag agtttaggga ttccttatag
     2221 cggaagtaat atgttatcta gtagtatatg tatggataag aatatttcga aaaagatttt
     2281 acgttatgaa ggtgtagaaa caccagattg gattgaactt acaaaaatgg aggatctaaa
     2341 gcttgatgag ttagataagt taggatttcc attagtggta aaaccaaact ctggcggatc
     2401 gagtgtcggg gtgaaaatcg tttacaataa aaatgaattg atctcaatgc tagaaactgt
     2461 atttgaatgg gattctgaag tagtaattga gaagtatgta aaaggtgatg aaattacatg
     2521 ttccattctg gatggaaaac agttacctat agtttcaatt cgacatgcag ccgagttctt
     2581 tgactacaat gcaaaatatg atgatactag tacagttgag gaagttatcg aacttccagc
     2641 ggaaattaat gaacgtgtaa ataaagcgtc actggcttgt tataaagcat taaaatgtag
     2701 tgtttatgca agagttgata tgatggtgaa ggatggaatt ccatatgtaa tggaaattaa
     2761 tacattacca gggatgacac aatcaagttt attgccaaaa agtgcagaag cagcgggaat
     2821 tagttatagc aaactattag atatgattat tgaaacatca ttaaaagtta gaaaagaaga
     2881 agggttttaa ggggattata taactaattc ttttgacagt ttcacgaagt atgctatgat
     2941 gaatacatta taaaaataaa atatttatcc actagggggg ccttttatag gctgagatca
     3001 aatgtgattt tgagactctt agtacctgat ctggttaatg ccagcgtagg gaagtgggaa
     3061 agacgttgct atttaatgag taaataaata ctgtcaattt cactttcttt acgcctgtaa
     3121 agaaagtttt tttattttac agctttaata attgtggata aatacttata ttttatacat
     3181 tattggaggg atttaaatga ctttttcaca atcattacgt aaagaagtag attcaatttg
     3241 ggaagcgagt tttaaccatc cttttgtaaa aaaacttggt gaaggaacgt tagatttagc
     3301 tagttttcgc tattatgtgc ttcaagattc atattatttg agtcattttg ctagagtaca
     3361 aactttagga gctgcaaaag ctcttgaatt agagacgaca gctcgtatgg cacatcatgc
     3421 tcaaaataca tatgaggccg agttatcttt acatgagaat tttgctaaaa agctagggat
     3481 aacaaaagaa gaaaaagata attttattcc tgcgccaact gcatatgcgt atacttcaca
     3541 tatgtatcgt gctgcgtatg aagggcattt aggagatatc attgcagcaa ttttaccttg
     3601 ctattggttg tattacgaaa ttggggaacg tttaaaagaa tgtcagccgg aagagccaat
     3661 ttataacgaa tggatttctg cttatggatc tgattggttc cgtacgctag ttgaagaaca
     3721 aattatacgg ttagatacta ttgctgaaaa agtaacagag gcagaccgca agcgtatgaa
     3781 acagcatttt attattagca gtcaatatga atattcattt tgggaaatgg cttatacatt
     3841 agaaaagtgg ccagtggata cagacgtaaa agacgtaatt ggataaagaa tagaagtaat
     3901 gttagagata ggtatggatc atacctatct ctatattttt tcttgtcaaa tttgcgtaaa
     3961 gtcattagtg ctacggatat ggtgaaatag aatatcgata atttagctca tttaattgtc
     4021 tacgcattaa ctggataaac ttctgtcaca tttattttac aattaaagaa cgtaaatgtt
     4081 ggtggtatga agttaaataa gaaaagaaac ccgagctgtt agctgtaacg gagttgtttt
     4141 ttctttgcag gaatttctaa gttaacatga agtgctgttc atacaatatg caaatttgca
     4201 tattgtgttt ttttgagttc tttgtgtgtt aaaatcaaat atagagatat ggaaatataa
     4261 agaagcaaaa gcaggaatat ccaatgaatt ttgagaggtg ttatatgttt acaaataaga
     4321 aattaattcg atttggttta tcgttgtttg tatttttagg gataattaat tttacaatca
     4381 gctatttcca aacatatctt gaaacagcag cagatattaa atgggtaatt ccagaaattt
     4441 ggaaaactat tttactagat gttcctcaag gtatacttgt tcttttaggc gcaattgcat
     4501 tatatgattt cacaaaagag gcatcacaaa aagacgcatc aatctaagtg tgtctttttt
     4561 caattagaaa tttcatagag agctttgctg gagaaataga ataaaaagcc ctaattaggg
     4621 cttttttctt tataggaatt tcttaaccgt catagaaacc ggttacttag aggtgttata
     4681 gcaaaggcat ttacagtaca agaaaatcta agtgatatat ggaaatatat aggatttgtc
     4741 cgcgtgatta ttgagaaatt aattgagctt agtaagtatg aagatatgac gctagaattt
     4801 atattttata atttctattt ataaaaatga gcccatagta aaaataggta tatgaacaag
     4861 gtgcatcatc tagtgataag tcatactttg ttggtgtacg tattgaactt agtttatagc
     4921 gctatttcta cttagatgta acgaacagaa atatagggaa gcatcaatga ggttgtattt
     4981 tataaacgga ggttatgaaa atgaatcagt ttcaacaaga aatacaagcg ttaagcctta
     5041 atgattaccg atctggaaat attgtctatt gggaccagca aaatcaatat ccatattact
     5101 atattgaaga tgctgctcgt cgctgtgggg gttgtggtcg ttgtggcggt tgtggcggag
     5161 gtcgttgtgg cggattccgt tgtggtggat tccgttgtat tggttgcttc ggttgttttg
     5221 gttgcggagg ttgtggtggt tgtggcggct gtggcggttg ctctaactgt tttgatggtt
     5281 ttagtggtac tactgatact actggtacta ttataacgta tgaatattga ttgcatttat
     5341 cctgctattt gccgggcagt aagaccctaa aaaaattcag cgagagcaaa gagaagttag
     5401 gtggccggct gcccataaaa gcccgatgga tgagggctaa ttaaagtttc accttatcaa
     5461 ttaaaaaaca ttagttttat atatgtacgt gccgacaaag aatatagtca ttattaatgg
     5521 atatattcaa tcattcaagt gaaagagtaa caataagata tataggtgta atcaagatgt
     5581 gatggataaa gcaatgacta gatttaaaat ctaatcattg tttttttctt atggttttat
     5641 cattaaatat ctgtctagtt tgggcattgc ttccttctag ctaatgattt tattacaacc
     5701 gtgtaaggtt aatgtaatgt taatcgatag taaaataagt tgcttgagtg tattttaaat
     5761 aagattaaaa agtactgttt atctataaaa taattgggta aataatatgt aattaggaag
     5821 tgtatgaata tggttgaact aaaatctatt ttccattcgt ataaagtaaa gagaaaaata
     5881 gctaaagatt tatacggaaa aagagatgaa ttgacactgt tattaaatga atttaataat
     5941 atgaaatgta cagtaacatc tgaaaagaag aaaaataata tattatctcg tttgcaacta
     6001 atttatcaaa atataaaatt agataagcag tatcctctat cagtttcttt tgatagtaaa
     6061 ttattggagc gattagaaaa agaatctcta catactattg aggatggtgt aacatgtcta
     6121 catttaatgt tagatatgaa ttatgaaaaa ataaaacaat atgggtcgag tacaagtagg
     6181 tcattcgttc cattatcgca gtcttctatt tgtcttgctg attgtatttg tttaacagga
     6241 tttatagtag gtttactagg agtaatttca tttggagggt tcatgttatc tatatgttca
     6301 actacataac gcttgatatt tgtaaaaaga gcaccgattt aaggtgttct tttttggttt
     6361 ttctgtaaag ttattgtaaa tgataagtac aatatattat atgtattata taatttaaat
     6421 gtattaaatt atgaatacta agaggtgttg tagataataa ttacttttaa tttttaaaag
     6481 agtcggtttg ataatgtttt atgtagatag tttttacttt tcttttaaat actttgttta
     6541 aatggattag attaagtaac ttttgttcta gctttttgat aatcaagaca actgaagaaa
     6601 ggaaagaatc attttatgaa aaaattaagg ttgttaacat ttgaaaatat agtagaacct
     6661 cttttaaatg aaaaggtatc atttatatac tttcctattg aatggctgga catcgtagag
     6721 atacattata agacgttttt attaacgagt aagttgaaac gtttgaatga aagactgtat
     6781 gatatgtttt ctgatatatt gtttattcag cataatccgt acgtattaaa tgaaaataca
     6841 ccatggattg tatcaaaaga acctattaaa caagagcagc tcaattatat ttttcaaagt
     6901 tggtatgaga ttattcatga ttggaaacca aataaattag tagagccacc aaaatatgaa
     6961 tggcaatccg atttgatttc taatttgcca gtactacatg ataatgaaac gtattctaag
     7021 tgggtgcccg ctttaatctc acatattttt tgtgagcgtc ctatatattt agaaaataca
     7081 aatgcagaag aaatctattt ttctcctctt agatcacaaa atatttgtga ggcgatgtca
     7141 gggccaataa aagatgaaaa aacacaagat tttttctcct atgtatatcg attcgaatgc
     7201 ataacccgtg gtggtgagaa cgctccatta ttaaatattt caatagggat tcggagattt
     7261 tatcaagaat ataagatgat aggtcaaaca aaccctgata tgacaacgtt tgtttgactt
     7321 tttttgataa aatacaaaca ttttgcaaac ataggttatc caaagatact tttaccgaag
     7381 tttttaacgg cttcttcctg catattcggt aaaacatgag aatagacact taatgtcatt
     7441 gaaatatctg tatgacctaa tctctcactg atgattttag ggttaactcc ttgtttcaat
     7501 agtatagttg cgtgtgtatg tcttaaatca tggaatttaa tttcttttat acctactttg
     7561 tgtgtcgccc taatgaaact ttttctgaaa tgtgctcttt ttatgattct tccaaactca
     7621 ttacaattta ttaaatcttg atctagataa gcagaaccaa accttaattt ctctttatta
     7681 attaaaatct tatgtttttt taaggctact attgtttcat taggtacagg aattgtgcgt
     7741 tttgacgaat ttgtttttgc agtttttttg attttattgt catgaccaga tgtttgattt
     7801 attgtaacgg tatgtttttc aaaatcaatg tcctgccatc gtaaccctag aacttctccc
     7861 agacgcatac ctgtagttat tgcaagtaga tacccaatgt gatatcgtga ttcttgtgaa
     7921 tgagctaaaa atttttttac ttcttcctct gtccaagtct ggataggggt tttttctttt
     7981 ttaggtatct cagcaaaatc tgctggattt cgagaaagaa tattttgttt tacagctagg
     8041 tttaaagcac tctttaaaat tctatgcata agcagaatgg aattatttgt aataccttta
     8101 tctatcgcag tcttatagca tttttgaatg tgcataacat ttaatttatg gagtgcgacc
     8161 attcctatac taggtataac atgttggttg ataaatgcct tatagccagc aaaggtactc
     8221 ttttgtatac taatactttt aatttctaac caatgattta ggtaatcttt tagcgtaact
     8281 ttagatggct ctataaaagt tccttcattc aattctgtaa tcttctttgc tacatcggcc
     8341 tgtgcttctt tttttgtctt atatccagaa aaccacttct gtcttctttt tcctgtctct
     8401 ggatcagggc cgatatcaat aactatgcaa tatttatttc ctctttttcg aatatgtcct
     8461 ttcacttaaa acactccttc attttgtttt gaatcatgtt gtataagtct agttgtaatt
     8521 ttgctgctat gaaaattaca tgtttggaca tatcagcgat ggatatattt taccatataa
     8581 aaataaattc agttatagtg taaggggatt catttgttgt taaacaatag tgaaatgcct
     8641 actccgatta ttaaatgcaa tattgcatat ttttttagga agtctaaaaa acaaggaata
     8701 tattaatgtt tttaactttc tcaagtattt tcagaatatg agagaataga tgtttgggtt
     8761 atttgtgata gaatattctt aataatataa tttgacggaa cgaaaaagac ccatagcgtg
     8821 tgtaattgtg ctgggaacac ttttacaccg tttgccctaa ttgtagtagg gaaaacattt
     8881 gccatgagcc ttttagttac gactatgcgt aacatatacg gctagtataa cacaactttt
     8941 agatgtaatt catcattaag gtgcgttttc atgataggga agtgtgtctt gttccaataa
     9001 gggggacaaa acatgtggaa agctctaaat caaattgaaa aagagttatg tgcagctgga
     9061 ataaggaaaa ataaactagc aaattattgg ggagttaagc caagtactgt tacaaaagtt
     9121 tttaaaggta acacagacat gagttttggc tttctttcta agacagtcat cctattaaac
     9181 aaaggcatac aggttcaaga aaatttatta acagattata taaatataac gaaaccaaaa
     9241 tcagaaaact tacgtgaggc aatggaggat ttagctttaa ggggaaagtt taatctacta
     9301 attaatatta taaatagtga atcgcaatca aaggtagcag aaaatagaga atttgctaat
     9361 gtgtatcgaa ttatatatag acgatatata ggtgagattg atgctataca gtaccataag
     9421 gcattaagct tcgaaagtaa atcaataaga acctcagaga tggaagtgtt aattgaaatt
     9481 ttattatgtc aagcgcaata tcaatcgggt aattttactt ctttaaatga gcgattaaaa
     9541 tctcttgaaa taaaactaaa taaaataagt aataggtaca ttcgagaatg ttataaatta
     9601 cgatataaag aagccattgc ggttacttcg ttgcagggtg gtgaagtcat tgaagcaagg
     9661 catgttagca tggaactatt agatgatctt gaatgggata actttttttc ttttccaaaa
     9721 gtaaatgcat atttaaagct aggagaatcg tatatttttt cagcgaatga atatgagaag
     9781 gcaaaatatt atttggaaaa gactcttgaa gtgctagggg atagtaagat taatggaatt
     9841 gagagaaaaa gaaaaatggt gcaacataca ttatcatttt taaagattca tcatgataaa
     9901 gaaattagta gcatagatgt tgttcatcca ggcgaactag catatttaag gattaaacaa
     9961 ggtaagagga ttgaagcgag aaaactattg attcaattaa aagagaagaa tggaaggtta
    10021 acagacatac agactgcata tttaggttta acatatgaag gtactaaaaa agaagagtta
    10081 atgaaacgtt ctcttttgat gtgtcaaaaa tcagggaata tattttattc gaatttacca
    10141 aaaatacact tgggtttaat ttgaataatt ggtataattg tcttcggaaa gaggtgaaat
    10201 aatgaaaaaa ataattacaa tcattcctgc gctattaata gctgctacat tattcattaa
    10261 cactgattct ataaaagaaa aacctagtac aaatgattta aaacctacaa ttcaacacat
    10321 gatggttgac
//
