LOCUS       NZ_NUIO01000034        14256 bp    DNA     linear   CON 20-MAY-2024
DEFINITION  Bacillus thuringiensis strain AFS049207
            AFS049207_100_C7_Contig45_140514, whole genome shotgun sequence.
ACCESSION   NZ_NUIO01000034
VERSION     NZ_NUIO01000034.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 14256)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14256)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Assembly Cell v. 4.2.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.45x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002575705.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 01:16:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,769
            CDSs (total)                      :: 5,694
            Genes (coding)                    :: 5,513
            CDSs (with protein)               :: 5,513
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 72 of 182
            Pseudo Genes (incomplete)         :: 113 of 182
            Pseudo Genes (internal stop)      :: 64 of 182
            Pseudo Genes (multiple problems)  :: 54 of 182
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:44:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 14256
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NUIO01000034.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..14256
                     /collected_by="AgBiome"
                     /collection_date="2014-01-14"
                     /country="USA: IA"
                     /db_xref="taxon:1428"
                     /isolation_source="excrement"
                     /mol_type="genomic DNA"
                     /organism="Bacillus thuringiensis"
                     /strain="AFS049207"
                     /submitter_seqid="AFS049207_100_C7_Contig45_140514"
     region          1..14256
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RRE-containing"
                     /region_number="1"
                     /rules="minimum(1, [Bottromycin_Methyltransferase_RRE,
                     Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /tool="antismash"
     cand_cluster    1..14256
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /kind="single"
                     /product="RRE-containing"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..14256
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[6355:7459](-)"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(6356..7459)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
     gene            complement(145..420)
                     /locus_tag="CN977_RS23410"
                     /old_locus_tag="CN977_23410"
     CDS             complement(145..420)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529644.1"
                     /locus_tag="CN977_RS23410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23410"
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="WP_098829684.1"
                     /transl_table=11
                     /translation="MGQFQSNLQTATQIATKMGSAPDRIQSATTRSITKATRTTLSVNF
                     KAQEANQQMLDLTKQFSAAFQQAVDNIHSVAKEFERMDNELHNTFR"
     gene            complement(583..>792)
                     /locus_tag="CN977_RS23415"
                     /old_locus_tag="CN977_23415"
     CDS             complement(583..>792)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918587.1"
                     /locus_tag="CN977_RS23415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23415"
                     /product="WXG100 family type VII secretion target"
                     /protein_id="WP_098829687.1"
                     /transl_table=11
                     /translation="LSQLQNLQNELRGEWEGRAFEGFDQQFNELKPKVQNFAQLLQEIN
                     MQLNKTAEAVASHDEELSRNFGLK"
     misc_feature    762..774
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 21.58"
                     /tool="antismash"
     assembly_gap    793..814
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(988..2349)
                     /locus_tag="CN977_RS23420"
                     /old_locus_tag="CN977_23420"
                     /pseudo=""
     CDS             complement(988..2349)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611143.1"
                     /locus_tag="CN977_RS23420"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="CN977_23420"
                     /product="IS3 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTKFTSEDNMNAVIHYQDGSESIKDIAKSLGANHEVVRMWIKQFE
                     YHGIQAFEKGYTTYSAQYKLDVLNYMFENGTSPNETAVIFNISSPGLIRKWRIQLHQKG
                     VDALKSKKKGRQSMTKDNQKSIKKQTQTEESVEALQAELERLRMENAYLKKLNALVQNK
                     EKSSNKIRRK"
     misc_feature    complement(2372..2385)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.94"
                     /tool="antismash"
     misc_feature    2373..2386
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.67"
                     /tool="antismash"
     gene            complement(2427..2756)
                     /locus_tag="CN977_RS23425"
                     /old_locus_tag="CN977_23425"
     CDS             complement(2427..2756)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566267.1"
                     /locus_tag="CN977_RS23425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23425"
                     /product="hypothetical protein"
                     /protein_id="WP_000566275.1"
                     /transl_table=11
                     /translation="MIDLLNKWMLESTGNFNIVVGITALLFLGSVIALIIIYKKIGKPD
                     ESTNAIYLKITSRMFTTQILMNAIFISLVGKDIENFRQIFILFEAFVFFIGAIYSFKLY
                     RQEYK"
     gene            3492..3641
                     /locus_tag="CN977_RS30425"
     CDS             3492..3641
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016097815.1"
                     /locus_tag="CN977_RS30425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000473828.1"
                     /transl_table=11
                     /translation="MFKKLVVGALATGISLIVSAFGTPFNNRMQVLAMLHSKFVEDSVL
                     VQKC"
     gene            complement(3681..>4014)
                     /locus_tag="CN977_RS23435"
                     /old_locus_tag="CN977_23435"
                     /pseudo=""
     CDS             complement(3681..>4014)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259899.1"
                     /locus_tag="CN977_RS23435"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="CN977_23435"
                     /product="DDE-type integrase/transposase/recombinase"
                     /pseudo=""
                     /transl_table=11
                     /translation="KVKGQWI"
     gene            complement(4096..4329)
                     /locus_tag="CN977_RS23440"
                     /old_locus_tag="CN977_23440"
     CDS             complement(4096..4329)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005923.1"
                     /locus_tag="CN977_RS23440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23440"
                     /product="hypothetical protein"
                     /protein_id="WP_001005924.1"
                     /transl_table=11
                     /translation="MNESVRKSEGELKKYEFTASELEMLFSGFYVAVRQLPENIGPKIY
                     EAIRCKSEEVLLKCLEESIRGSEEKKKEKSGF"
     misc_feature    4371..4384
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.4"
                     /tool="antismash"
     gene            complement(4610..5089)
                     /locus_tag="CN977_RS23445"
                     /old_locus_tag="CN977_23445"
     CDS             complement(4610..5089)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014471322.1"
                     /locus_tag="CN977_RS23445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23445"
                     /product="DinB family protein"
                     /protein_id="WP_001033113.1"
                     /transl_table=11
                     /translation="MNKIELIIMNSNEVRRRSLNLWSSIPSEVLFHRVDSSSMTILEQV
                     RHVLEAEYLYLEILKTRKSLPYSDDEPNPFNTRDFTTIQDEVDFAKPYRKNFLDFIMGL
                     NEEDLENIKIDRSDVGYTRNLGDMLMRMAYHEAVHTGQLLRDMRALGLKRPHLWD"
     gene            complement(5117..6349)
                     /locus_tag="CN977_RS23450"
                     /old_locus_tag="CN977_23450"
     CDS             complement(5117..6349)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937089.1"
                     /locus_tag="CN977_RS23450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23450"
                     /product="MFS transporter"
                     /protein_id="WP_000937089.1"
                     /transl_table=11
                     /translation="MLPKVFLNKNYVILFSGMTISKLGMWFFTIAIPLIVYDLTHSPSK
                     MAFVLALELGAQVIFSLIGGALADQMSKKKIMIIGDYASALFVLVVPILFLSGYNSIIV
                     IYIAAFTLSALAAFHHPSFESAVPEILSKEDLIQGNSFFRLSETIITFLGPAVAGIAIA
                     LFGNSNVLLITSVAFFLSGTIFLFLKFRDFAEEEKKRPELVKPIVEGLRYVFGTKIILV
                     GSLVIFGINIGFGAVESLFMYYLKDYLGLSPINIGLIFSLQAIGPILAVYFASKFKHIP
                     RGNVIIYSGIVIGLAQMLLYFSQFSLVILVFCQIFIKGSVTLLAINWFTLRQETVPSKL
                     LGRVISSTRMVAFLALPFSAMIAGFLAESINVLSIFLVAGAFAIVSSIVGVSLGLFDRK
                     RLDKIQNDTAL"
     gene            complement(6356..7459)
                     /locus_tag="CN977_RS23455"
                     /old_locus_tag="CN977_23455"
     CDS             complement(6356..7459)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Pantocin_Microcin_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ThiF"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802791.1"
                     /locus_tag="CN977_RS23455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23455"
                     /product="ThiF family adenylyltransferase"
                     /protein_id="WP_098829685.1"
                     /sec_met_domain="Pantocin_Microcin_RRE (E-value: 2.6e-30,
                     bitscore: 94.5, seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="ThiF (E-value: 1e-46, bitscore: 149.1,
                     seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPKFKETIPYFFLNKEIQIGEEEGLAGIIPDPTGSISFLINKLD
                     GQRNIPNIVQEVQKEFPDISSNEIIEAIKVLGNEGYLEDNSIVPELNDYTLERYKANIN
                     YFSLFTKFGEDKYKIQEKILETPIALLGVGGLGTQVLYHLAALGFHNIKALDFDNIELS
                     NFNRQLLYSESDIGNSKVEMAKKRISQFNPNVDLQIINKKIESPQDVIEHIEGTELVIC
                     VADKPTLHIANWVNEGVVKCNLPMVYGGVLNTRGRFFSMIPSKTGCVQCHINYARQLND
                     KQDEQLEAMNNMEFTRNNAAISPNVAIVAGTIVNEALKILTQIAQPISLGKVMEVDFLT
                     LETNTVSSWEKMLDCPLCGQVVKSEVK"
     aSDomain        complement(7175..7459)
                     /aSDomain="Pantocin_Microcin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing E1-like protein in a
                     pantocin/microcin cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_CN977_RS23455_Pantocin_Microcin_RRE.1
                     "
                     /evalue="5.20E-30"
                     /identifier="RREFam017.1"
                     /label="CN977_RS23455"
                     /locus_tag="CN977_RS23455"
                     /protein_end="95"
                     /protein_start="0"
                     /score="94.5"
                     /tool="antismash"
                     /translation="MKPKFKETIPYFFLNKEIQIGEEEGLAGIIPDPTGSISFLINKLD
                     GQRNIPNIVQEVQKEFPDISSNEIIEAIKVLGNEGYLEDNSIVPELNDYT"
     misc_feature    7695..7709
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.5"
                     /tool="antismash"
     gene            complement(7752..8552)
                     /locus_tag="CN977_RS23460"
                     /old_locus_tag="CN977_23460"
     CDS             complement(7752..8552)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016097814.1"
                     /locus_tag="CN977_RS23460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23460"
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="WP_000394562.1"
                     /sec_met_domain="PEP_mutase (E-value: 5.7e-65, bitscore:
                     209.0, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFKEKIKLFRTLHDQNNPLMLPNAWDASSAKIFERNDFQAIGTT
                     SAGIAATLGYPDGEKIPLELMLNSISNISKNTKLPVSADIEGGYAANIFDLRKILNELE
                     KFEVVGINIEDGRGTYLREINEQKVILDFIKAYSPHLFINARIDSFWLDIGDLKDRIKD
                     VIVRAEEYKKSGADCIFIPGVKDIETIKVLRENIELPINLLQSENLNSMQNIKTLKIER
                     VSLGSSAFRNILTNLDKISSTFKQGKLLHSNGNLTYQDLNLLMD"
     misc_feature    8593..8608
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 23.85"
                     /tool="antismash"
     gene            8675..10576
                     /locus_tag="CN977_RS23465"
                     /old_locus_tag="CN977_23465"
     CDS             8675..10576
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NpaA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684559.1"
                     /locus_tag="CN977_RS23465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23465"
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="WP_098829686.1"
                     /sec_met_domain="NpaA (E-value: 2.5e-188, bitscore: 617.0,
                     seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIAIVGLGPRGISVFERIVSNILSELKSESYEFFLIDPHPPGGA
                     VWRTDQSPQLLMNTVASQITLFTDDSVQCRGPIVNGPSLLEWANELRDKIDTLDMPTVI
                     TEEIYSLEPNSYPTRALYGYYLKDSFNTILEELPSNIKINLIQDTLINLYPNNEDYTLF
                     LNKSLTPIKAEKVIFTTGHSDQKLTSAEEHFFEFANSNNLNYFPPSNPADVNLDSIEPK
                     TPVIIRGLGLNFFDYMSLLTSGRDGKFEEINDKLNYIPSGKEPLLIAGSRRGLPYHSRG
                     QNQKGSSERHIPLFLKNEVIEQLKHKNNLSFKKEIWPFVKLEVESIYYTTLIRNLYGIE
                     KSCQFLYEFVNCAIEYHKDLLINFGVKSELFFDWHKISCPYGDKKFNSITDFHNWLDTY
                     LHNDINEANLGNKNSPLKAALDVLRDIRNEIRILVDHGGINAESYKEELRNWYTPLNAF
                     LSIGPPVQRIKELRALIRAGILVLVGPEATVATSPINKDFKISSNRVKDSIFHAKNLID
                     ARLPSVNINQSKNILISNMLKAGLASEFKIATNNNYIFESGGLAVTPPPNRLIDKNGKV
                     NPNLYSFGIPTEGVHWVTAAGARPGVNSVTLLESDSIARDVLGCNITINNVYKQTIS"
     gene            complement(10612..11967)
                     /gene="purB"
                     /locus_tag="CN977_RS23470"
                     /old_locus_tag="CN977_23470"
     CDS             complement(10612..11967)
                     /EC_number="4.3.2.2"
                     /GO_function="GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA]"
                     /GO_process="GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="purB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089379.1"
                     /locus_tag="CN977_RS23470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23470"
                     /product="adenylosuccinate lyase"
                     /protein_id="WP_001089379.1"
                     /transl_table=11
                     /translation="MNSTPIDSEIFKDQFGTKQMRTNFSDENLIQLWLNSEVALAKAEA
                     KLGIIPQNAATNIANAADQNKLDFNKIREGMKKTGHPFVAFLHEFESLCDEESRKYIHW
                     GATTQDIIDTGLTLQIKGAHELIYNQTQEILKICLRLSQQYKSTVMPGRTLGQHALPIT
                     FGYKISIWSDELGRHLERLQQGADRYLVGQLGGAVGTLASLGAQGLAVRKEYVKNLNLI
                     EPLVSWHTSRDRFAEFSMILNMIGATTAKVAEEIISLQGTEIREVEEGYHSGKVGSSTM
                     PHKRNPMTCSYIVGLTKIVNRKSTLAIDSMIQKHERDVTFWQSEWSYISEICITLSGAL
                     EQLKNVLTNLIVNEEQMKKNLFLTKGLIVSENVMLELGKIIGRTTAHDLLYELTMRAFE
                     EDKDLKDILLEDNEIKKYFSDEEINSLTNPESYTGLSEKFVDDVYNKWLSYT"
     misc_feature    complement(12505..12521)
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 22.72"
                     /tool="antismash"
     CDS             complement(12813..>14254)
                     /NRPS_PKS="Domain: PCP (6-57). E-value: 2e-10. Score: 32.7.
                     Matches aSDomain: nrpspksdomains_CN977_RS23475_PCP.1"
                     /NRPS_PKS="Domain: NAD_binding_4 (95-342). E-value:
                     3.3e-49. Score: 159.2. Matches aSDomain:
                     nrpspksdomains_CN977_RS23475_NAD_binding_4.1"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895184.1"
                     /locus_tag="CN977_RS23475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23475"
                     /product="thioester reductase domain-containing protein"
                     /protein_id="WP_141526699.1"
                     /sec_met_domain="PP-binding (E-value: 3.3e-09, bitscore:
                     27.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 7.7e-50, bitscore:
                     159.2, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LGIDSSVIGINDNFFDLGGHSLKIMSTLVKTFSEGWNVTIKDYFE
                     LKTINNIAKKIQYGYKVNSHSDTLDIKLTTPPKKLKKTKRHEFSNNSKILLTGATGFLG
                     IHLLEQLLDTTECKIYCLVRGENKQEANNRLLHMLKFYFKNKFVSYQSLVNQRIFIVQG
                     DLAKQNMGLENELYKELHQQISTVIHAAALTKHFGEYSEFERANVQAVKELLAFVGDTK
                     DLHHISTTGVAGQFVLNETETIFKESDFYVKQNYKDNVYVKSKFLAEYEIFKAILTGTD
                     ATIYRVGNLTNRYIDGQHQYNFTENAFMSKLKFILQYGIVTDVLLSSEVEFTPVDYCSK
                     IITGFVTSNEIHENSKNCVNHIYNHLTLNLNVLVKLLNSMGYPIKVISETEYQQFILEL
                     SKDKKKQEDIQNLMTFEEPKDNCYKPINLDSTETQKTLKLLGIQWPIINIEYIQQIINY
                     MTSVTGSNSVAKLFKQSYTLKN"
     gene            complement(12813..>14256)
                     /locus_tag="CN977_RS23475"
                     /old_locus_tag="CN977_23475"
     aSDomain        complement(13227..13967)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CN977_RS23475_NAD_binding_4.1"
                     /evalue="3.30E-49"
                     /label="CN977_RS23475_NAD_binding_4.1"
                     /locus_tag="CN977_RS23475"
                     /protein_end="342"
                     /protein_start="95"
                     /score="159.2"
                     /tool="antismash"
                     /translation="LTGATGFLGIHLLEQLLDTTECKIYCLVRGENKQEANNRLLHMLK
                     FYFKNKFVSYQSLVNQRIFIVQGDLAKQNMGLENELYKELHQQISTVIHAAALTKHFGE
                     YSEFERANVQAVKELLAFVGDTKDLHHISTTGVAGQFVLNETETIFKESDFYVKQNYKD
                     NVYVKSKFLAEYEIFKAILTGTDATIYRVGNLTNRYIDGQHQYNFTENAFMSKLKFILQ
                     YGIVTDVLLSSEVEFTPVDYCSKII"
     aSModule        complement(13227..14234)
                     /domains="nrpspksdomains_CN977_RS23475_PCP.1"
                     /domains="nrpspksdomains_CN977_RS23475_NAD_binding_4.1"
                     /incomplete
                     /locus_tags="CN977_RS23475"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        complement(14082..14234)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CN977_RS23475_PCP.1"
                     /evalue="2.00E-10"
                     /label="CN977_RS23475_PCP.1"
                     /locus_tag="CN977_RS23475"
                     /protein_end="57"
                     /protein_start="6"
                     /score="32.7"
                     /tool="antismash"
                     /translation="VIGINDNFFDLGGHSLKIMSTLVKTFSEGWNVTIKDYFELKTINN
                     IAKKIQ"
ORIGIN
        1 tgttcttgag cttgaatcgc gaattgattt cggtcctgtt cttcaaatac atttcgtaac
       61 tttagattta actctttaat tttattttca atatctcgac ccatcttttt cctcctacta
      121 ttttacttta cgtaggttgt cacatcagcg aaaagtattg tgaagttcgt tatccattct
      181 ctcaaattct ttagctaccg aatgaatatt atcaaccgct tgttgaaagg cagcggaaaa
      241 ttgtttcgtc aagtctaaca tttgttgatt cgcttcttgt gctttgaagt taacagataa
      301 tgttgtacgc gtcgcctttg ttatagaacg agttgttgca ctttgaatcc tatcaggggc
      361 tgatcccatt ttcgtagcaa tttgcgttgc tgtttgtaaa ttactttgaa attgtcccat
      421 tctttcaaac cctttctata agttttccaa acgcatcaaa gaaatctcat aaaatatata
      481 tccctatcaa tatcactcgc tctaggcatg caattaaatg agccatacaa tataagctgt
      541 atggctcatt tgaaatgctt gttatataga gtatgacaat ttttatttca aaccgaaatt
      601 acgtgataat tcttcatcat gagaagcaac agcttctgca gttttattca gctgcatatt
      661 aatttcttgt aatagctgtg caaagttttg tacttttggc tttaattcat tgaactgttg
      721 atcaaaacct tcgaaagcac gaccttccca ttctcctctt aattcatttt gcaagttttg
      781 tagttgactt aannnnnnnn nnnnnnnnnn nnnntattgg ttaattttac taaatatatt
      841 ataatgtatt attttgaact tttaaacaat ttatctttct attgaatatt agaatattaa
      901 tattctactt tttcttgctt tacaaggttt tatgcaaaaa agaccaccaa aatgaactga
      961 ccccataaag ttagacaggt cgtttcatta ggcagccttt agggcatgag ttcgatattg
     1021 taccggactc atgcctttta gtttttcctt aattcttttg tgattataat aatcgatata
     1081 tttttcaaat tcttgtttaa agtgttctat gctttcaaaa tcttttagat aaagaaattc
     1141 agatttcata ataacaaaga aactttcaat aaccgcatta tcgtaacagt tccctttacg
     1201 agatatactt tgcgtaatct tgcgttcctt tagggcatga cgatacttct tcatttgata
     1261 atgccagcct tgatcagagt gaataaggag ttcgtcttct tctgtcaatt gttgaaaaga
     1321 cttctccaac atcttcgata caagtgaata ggttggtctt gaatcaattg tataagcgat
     1381 tatctctcca ttaaatagat ctaacatcgg tgataagtag agcttctcgc cacataattt
     1441 aaattctgtt atgtctgtaa cccatttttc gttcggcttt tcagctttaa aattacgttc
     1501 taaaatattt ggcgcaatct tacctacttg tcctttatac gagcgatatt ttttcatacg
     1561 aactagactc tttaaaccta actctttcat aattcgttga acttttttat ggttgatttt
     1621 gtgtccttcg ttcattaact catcgcgaat acggcgatat ccataacgac cttggtgttc
     1681 atcgtaaatg ttttgaatta aggatcttta gctctttatc tttatctggt cgattagaat
     1741 tctgtatcca gtaataatat gtacttcatg ggagttctgc taattttaaa agtgcctgca
     1801 ccggaaatgc tttccttagt tcatagatta cttgcgcctt atcttgtttg acgatttttc
     1861 cttgttttga actaaggcat tcaacttttt taaataagca ttctccatac gcaaacgctc
     1921 caattcagct tgtaatgctt ctactgactc ttctgtttgt gtttgtttct taattgattt
     1981 ttgattgtcc ttagtcatag attgacgccc ctttttcttc gatttaaggg cgtctactcc
     2041 tttttgatgt agctgaatac gccattttct aataagtcca ggtgaagaaa tattaaaaat
     2101 gactgccgtt tcgtttggag acgtcccatt ttcgaacata taatttagta cgtctagttt
     2161 atactgtgca gaatacgttg tatagccttt ttcaaaggcc tggattccat gatattcaaa
     2221 ttgttttatc cacatgcgaa ccacttcgtg attcgctccc aatgatttcg caatatcttt
     2281 tatactctca cttccatctt gataatgtat tactgcattc atgttgtctt ctgaagtaaa
     2341 tttagtcata aaaaaactac acctccaatt gttagactgt gtctaacaat tggggtgcag
     2401 tttttttatt gtttcttgca agtaaattat ttatattcct gtctatataa tttaaacgaa
     2461 taaatagctc caataaaaaa tacaaaagct tcaaataata taaagatttg acggaaattc
     2521 tcaatatctt taccaactaa agaaataaat atagcattca ttagaatttg agtagtaaac
     2581 atacgagaag taatttttaa ataaatagca tttgtgcttt catctggttt accaattttt
     2641 ttgtaaataa taataagtgc aattactgaa cccaaaaata gaagtgctgt aattcctaca
     2701 acgatattga aattgcctgt actttctaac atccatttat ttaatagatc aatcattatt
     2761 atcttccttt cctacataag agaatatttc gtccttcttt ttcactataa taaaaaaaga
     2821 aaaaggaaaa catcgaactt tcttacaaac attcgtgtat tcttacagtt ttgtcatttt
     2881 gtaaatgtat tcatcgcctt gcctttcact attttctttg gaaaacatag tgaattacga
     2941 gaaagagcgc tacaagatca acttccaagg agtagtgcac taaaactctt aattaatgca
     3001 attagtttgt tttaaagtag taagtggaca atacttctta gttgtagaaa gtatcgagat
     3061 ccctatgcca attccagcgt ttgctgcagc tctattaatt ggtttaggaa taatcccgct
     3121 ttgttcttaa tttaaggaac tccaaacagc cctacctttc aattgataaa ccctcaagtg
     3181 tatgaatgct tggaggtttc ttataaaaaa aagacgccat aaggcgcccc cacaattggg
     3241 tagataagac tttagttttc caatctaaag aagttttgta tcagctcttt gagagtatat
     3301 cataatattc ggttcattta ttatgtattt ttacatacct ataaaaatcc ctctattttc
     3361 cacttaataa aatgaattac caatattaat aagattcaaa taaacatttc tacaaattca
     3421 aaacatttac aaaataagaa aagtgatata tttaaggtgc aagtcataaa aaatattaag
     3481 ggggatttat tatgttcaag aaattagtag ttggagcatt agcaactggt attagcttaa
     3541 ttgttagtgc atttggaaca ccgtttaata ataggatgca ggtgttagcc atgttacata
     3601 gtaaattcgt tgaagattcg gttctggtgc aaaaatgtta gttggaaaca tagaatagca
     3661 attttctctt catttctatc ttatgaagct aagccaaacc atttaggaat aaattctttc
     3721 tgattttgga cagacttgtc cggtaaaaca agttgcccgt tttttatcat atgcatagat
     3781 tcgacaccac tcaatataga gattgctgtt ttaaatgact tgaatctcta caggataggc
     3841 agggttctta tctactgtta tcacacgagg tttagaaaca tgcgaaaaag ccaaggcttt
     3901 cttgaaaaag cgcttgactg ctggtttatc tcttgattta cttagataaa aatcaattgt
     3961 attaccctct gaatcaactg cacaatataa ctatatccat tgacctttta cttttctttc
     4021 aacttaggtc ttatatcttt ctgtaacgtt tgtgagggat ttaagtgtca attctttgta
     4081 cgataaattt caatgttaaa atccgctttt ttcttttttc ttttcttcag accctcttat
     4141 tgattcttct aaacacttca ataatacttc ttcacttttg catcgaatag cttcataaat
     4201 tttaggtcca atgttctcag gcaattgcct aactgcaaca tagaatccac taaacaacat
     4261 ttctaattca cttgctgtaa actcatactt ctttaactcg ccttccgatt tgcgtacaga
     4321 ttcattcacc agagcattct gtgttatatc taacaaacat attaaataat cgtataaact
     4381 tgcattttct tatatatatt caataacttg caaacgttca ttaggaaatc ttctcaacgc
     4441 ttccattttt atctgtctat aactcggagt gtgctcatga atatctgtta aaaactcttc
     4501 agtgctcata actcttcccc ctacatactc atgttatata ttttaacatg tataactcgt
     4561 cagaaataga aacatttatt tagaatgcta acggataacc atttttaatt taatcccata
     4621 gatgaggtct ttttaaacct aaagccctca tatctcttaa taattgccct gtatgtacgg
     4681 cttcatgata cgccattctc attaacatat ctccaaggtt tctagtatat cctacatctg
     4741 agcgatctat ttttatattt tcaagatctt cctcatttaa tcccattata aaatctaaga
     4801 aattttttcg ataaggtttt gcaaaatcaa cctcatcttg aatggttgta aaatcgcgtg
     4861 tattgaaagg atttggttca tcatcgctat agggcaaact ttttctagtc tttaaaattt
     4921 caagatataa atattcagct tctaaaacat gccgtacttg ttctaatata gtcattgaag
     4981 atgaatcaac cctatgaaat aaaacctcac tgggaataga actccataaa tttaaacttc
     5041 tccggcgtac ttcattagaa ttcataatta ttagttcaat tttgttcata aaataactcc
     5101 ctttacactt aactaattac aatgctgtat cattttgaat cttatctaat ctttttctat
     5161 caaataaacc aagacttaca cctacgattg atgatactat cgcgaaagca cctgcaacta
     5221 agaagattga caaaacattt attgactcag ccaaaaagcc agctatcata gcactaaaag
     5281 gtaaagctaa aaatgctacc attctagtag aactgataac tcttccgagc aacttgctag
     5341 gtacagtctc ttgtcgcaat gtaaaccaat taatagctaa taaagtaact gatcctttaa
     5401 taaaaatttg acagaatact aaaattacta agctgaattg agagaagtac aggagcatct
     5461 gggcaagtcc tataacaatc cctgaataaa taataacatt tcctcttgga atatgtttaa
     5521 atttacttgc aaaataaaca gccaaaattg gtccaattgc ctgtaatgaa aaaattaatc
     5581 caatatttat aggactcaag cctagataat ctttcaagta atacataaaa agagattcta
     5641 cagctccaaa tcctatatta ataccaaaaa taactagaga accaactaag ataattttcg
     5701 ttccaaagac atatcttagt ccttcaacaa taggttttac taattcaggg cgtttcttct
     5761 cttcttcagc aaaatctcta aattttaaaa ataaaaatat tgttcctgat aaaaagaagg
     5821 ccacacttgt tattaataaa acattcgaat tcccaaaaag cgctattgca atccccgcta
     5881 cggcaggtcc caagaatgtg ataatcgttt cgcttagtct aaagaatgag ttaccttgaa
     5941 taagatcctc tttagataag atttcaggaa ctgcagattc aaaactagga tgatgaaaag
     6001 ctgctaaagc agataaggta aaagcggcaa tatatataac tattatgctg ttataaccac
     6061 ttaaaaaaag tataggaact acgagtacaa aaagcgctga agcatagtct cctattatca
     6121 ttatcttctt tttagacatt tggtctgcta atgccccacc tattaaacta aaaataactt
     6181 gggctccaag ctctaatgct agtacgaagg ccattttact tggagaatgg gttaaatcat
     6241 aaacaattaa tggaattgct atagtgaaga accacattcc taatttactt attgtcatcc
     6301 cagaaaaaag tattacataa ttcttattta aaaaaacttt aggtagcata tcaaatcact
     6361 tcacttcact tttaactact tgaccacaga gaggacaatc taacattttt tcccacgaag
     6421 aaacagtatt agtttctagg gttaaaaagt ccacttccat aactttccct aaagaaatag
     6481 gttgtgcaat ctgcgttaaa atttttaatg cttcattaac aattgttcca gctacaatag
     6541 ctacgtttgg acttatagca gcattgtttc tagtaaactc catgttattc atagcttcta
     6601 attgctcgtc ttgtttatcg tttaattgtc ttgcataatt aatgtgacat tgtacacaac
     6661 ctgtcttaga aggaatcata gaaaagaatc taccacgagt atttaatact ccaccataaa
     6721 ccattggcag attacattta accacacctt cgtttaccca gtttgcaata tgtaatgttg
     6781 gcttatctgc aacacatatc acaagctcag ttccttcaat atgttcaatc acatcttgag
     6841 gtgactctat tttcttattt ataatttgaa gatccacatt agggttgaat tgggatattc
     6901 gctttttagc catttcaacc ttactattcc ctatatctga ttccgaataa agcaactgtc
     6961 tattaaaatt acttaattca atattatcaa agtccagagc ttttatatta tgaaatccta
     7021 atgctgccaa atgatacagt acttgtgttc caagtcctcc tacaccaaga agtgcaatag
     7081 gcgtttctaa aatcttttct tgaattttat atttatcttc tccgaatttc gtgaatagtg
     7141 aaaaataatt tatattcgct ttatatcttt ctaaggtgta atcattgagt tctggtacaa
     7201 ttgaattatc ttctaaataa ccttcatttc ctaaaacttt tatagcctca ataatttcat
     7261 ttgaactaat atcaggaaat tctttttgta cttcttgaac gatgtttgga atgtttctct
     7321 ggccatctaa tttatttatt agaaatgaga tgctaccagt agggtctgga attatacctg
     7381 ctaaaccttc ttcttcacca atttgaattt ctttatttag gaaaaaataa gggatagttt
     7441 ctttaaattt tggtttcatt ttataacatc tccttaatta attgattaat caaaaaatta
     7501 agaagaaccg gaaatccggt tcttcttaat gacttaacct ggtacattag taagtgcatt
     7561 agcagcaact acttttttag aagctttttt tactttaacg atcatatgta atcacctcct
     7621 acatttgata aatctagcat aacacacaaa aaagagaaat tacataatag tctgatagtt
     7681 tcaggttgag aaactttttt ttgaaaatta tagaaataac tatttttagt agtttattca
     7741 cctgtaaaaa atcaatccat aagcaagttt aagtcttgat aggttaagtt tccatttgaa
     7801 tgtaataatt tcccctgttt aaaggtgctg cttatcttat caaggtttgt tagaatattc
     7861 ctaaaagctg aagatccaag actcactctc tcaattttta gtgtctttat gttttgcatg
     7921 gaatttaagt tttcactttg aagcaaattt ataggtaact ctatattttc tctaagtact
     7981 tttattgtct caatatcctt aactccagga ataaaaatac aatctgctcc ggatttttta
     8041 tattcttctg cacgaacgat tacatcctta atacgatctt ttaaatcgcc aatatctagc
     8101 caaaaactat caattcttgc atttatgaaa agatgaggcg aataagcttt aataaagtct
     8161 aaaatgacct tttgctcatt aatttctctt aaatacgtac ctctcccatc ctctatattt
     8221 atacctacca cttcaaattt ttctaactca tttaatattt ttcgtaaatc aaaaatatta
     8281 gcggcatatc ctccttcaat atcagctgaa acaggaagtt tggtattttt cgagatatta
     8341 ctaatggaat ttaacattaa ctctaaagga atcttctcgc catctggata acctaaggtt
     8401 gctgcgatac cagcactagt agtcccaatc gcttgaaaat cgtttctctc aaaaatctta
     8461 gcacttgatg catcccatgc atttggaagc attaatggat tgttttgatc atgtaacgtt
     8521 cgaaataact taattttctc tttaaattcc aatttaaaca ccctttagta aattattttt
     8581 ttagttttac acttgacttt ttttgcacct actagttatg ttgttttaca taacggatta
     8641 aattagatct tgcagaaagg ataggtgtga gtatatgaaa atcgcgatag taggtttagg
     8701 accaagaggt atatcagtat ttgaaagaat agtctctaat attttatctg aattaaaatc
     8761 agaatcttat gaattttttt tgattgatcc acatcctcca ggaggagctg tatggagaac
     8821 agaccagtca cctcaacttt taatgaatac agtggcttcg caaatcactt tatttactga
     8881 tgatagcgtc cagtgtcggg gtcctatcgt taatgggcct agtctattag aatgggcaaa
     8941 tgaattaagg gataaaattg acactttaga tatgcccacc gttattacag aagaaattta
     9001 ttccttagaa cctaactctt accctacaag agctctatac ggctattatc ttaaagattc
     9061 atttaatact attcttgaag aattaccctc aaatattaag attaacttaa ttcaagacac
     9121 attaattaat ctctatccga acaatgaaga ttatacttta tttcttaata agtctttaac
     9181 accaattaaa gctgaaaagg ttatctttac aactgggcac tcagatcaga aattaacttc
     9241 agctgaagaa catttctttg agtttgctaa tagtaacaac ctaaattact tcccaccatc
     9301 caatccagca gatgttaatt tagattcaat agaacctaaa actcccgtaa taataagggg
     9361 attaggcctt aatttttttg attacatgtc acttttaaca agtgggagag atggaaagtt
     9421 tgaagaaatt aatgacaaat taaattacat tccctctggt aaagaacctt tattaattgc
     9481 aggttctagg cgaggcttac cttatcactc taggggacaa aatcaaaaag gttcttcaga
     9541 aagacatatc ccactatttc taaaaaatga agttatcgag cagttaaaac ataaaaataa
     9601 cctaagcttt aaaaaagaga tatggccttt cgtaaaattg gaagttgaaa gtatctatta
     9661 tacaacttta attcgcaatc tctatggaat agaaaaatct tgtcagttcc tttacgagtt
     9721 tgttaattgt gccatagaat accataagga tcttttaatc aactttggag taaaatcaga
     9781 actatttttt gattggcaca aaattagttg tccttatggt gataaaaaat ttaattcaat
     9841 aacagacttt cataattggt tagatactta cttacataat gatattaacg aagcaaacct
     9901 gggtaataaa aacagtccac taaaagccgc attagatgtt ttaagggata tccgaaacga
     9961 aataagaata cttgttgatc atggaggaat aaatgctgaa tcttataaag aagagttacg
    10021 caattggtat acacctttaa acgcatttct ttcaattggc cctcctgttc aaagaatcaa
    10081 agaattgagg gcattaataa gagccggaat tttagtttta gttggacctg aagcaactgt
    10141 agctacttca cctataaata aagattttaa aattagctct aaccgtgtta aggattcgat
    10201 atttcatgca aagaacctaa ttgatgctag actaccctca gttaatataa atcaaagtaa
    10261 aaatatttta atctcaaata tgttaaaagc aggtctagcc tcagaattta agattgcaac
    10321 gaacaataat tatatttttg aaagtggagg tttagcagtg actccccccc caaatagatt
    10381 aatagataaa aacggtaaag ttaatcctaa tttatactcc tttggaattc caacagaagg
    10441 cgttcattgg gtaactgctg cgggagcgcg accaggggtt aattctgtaa cactccttga
    10501 atccgattca attgcaagag atgttctagg ctgcaatatt acaattaata atgtgtataa
    10561 gcagaccatt agctgaggaa ttattcctca gctaatggtc ttaaattggt tttatgtata
    10621 actcaaccac ttgttataaa catcatcaac aaacttttca ctcaaccctg tgtatgattc
    10681 tggattagta agggaattta tctcttcatc gctaaaatac ttcttaattt cattgtcttc
    10741 taataaaata tcttttaaat ctttgtcttc ttcaaaagct ctcattgtta attcgtacaa
    10801 taaatcatgg gcagtagttc taccaattat cttccccaat tctaacatta cgttctcaga
    10861 tactattaaa cctttggtaa gaaaaagatt ctttttcatt tgttcttcat ttactattaa
    10921 gtttgttaag acatttttaa gttgttctaa tgcgccgctt aaagttatgc aaatttccga
    10981 aatataagac cattcagatt gccaaaatgt aacatctctt tcgtgtttct gaatcatact
    11041 gtcaatagct aaagttgatt ttctattgac tattttagtt aatcccacaa tataagagca
    11101 agtcatagga tttcttttat gcggcatagt gctacttcct actttaccag aatggtaccc
    11161 ctcttctact tctctaatct ctgttccttg aaggcttatt atttcttctg caactttagc
    11221 tgtagttgct ccaatcatat ttaaaatcat gctaaattct gcaaatcggt ctctagacgt
    11281 gtgccaagat actaagggtt caattaggtt taaatttttt acatactctt ttctaacagc
    11341 taacccttgc gctcctaatg aagctaaagt accaactgca cctcccagtt gccccactaa
    11401 atatcggtct gcaccttgtt gtaacctttc taagtgacga ccaagttcat ctgaccatat
    11461 agatatctta tatccaaaag taatcggtaa ggcatgctga cctaacgttc tgcctggcat
    11521 tactgttgac ttatattgct ggcttaatct caaacatatc tttaatattt cttgagtttg
    11581 attataaata agttcatgag cacctttaat ttgcagggta agtcccgtgt caattatatc
    11641 ttgtgttgtt gctccccaat gaatgtactt acgtgattct tcatcacata aactctcgaa
    11701 ctcatgtagg aatgctacaa aggggtgtcc agtttttttc attccttctc taatcttatt
    11761 aaaatcaagt ttattttggt cagctgcatt tgcaatatta gtcgctgcat tttgaggtat
    11821 aatacctaat ttagcttcag cttttgctaa ggcaacttct gaattaagcc acaattgtat
    11881 taaattctcg tctgaaaaat ttgttctcat ttgttttgta ccaaactggt ccttaaagat
    11941 ttcagaatca attggtgtcg aattcattag tatcaccttt ctttttcatt aatttaagat
    12001 aaaatatcta ctattgatat tagcatatta atgtaagtaa tttgtcttaa aattgatttt
    12061 atatattttt gaaacctaaa atgaaaagga atacgggaac aaaagaacaa aaaaacacaa
    12121 cagataaaag attcttttcg taacaaacga agggatcttt tatctgttgt aagaaccatg
    12181 ggttcccaag aatccttcac ctatcattta ttcatttcct gttcccaatt atcaacttaa
    12241 atatccgcta ctccctgttc caatggctac acatctcctt ttgttactgt agcttgttca
    12301 tataacgtgt ttaacgatgt tattccccat aaagaaaaag ataatccact taaaattgta
    12361 atctcgatga cagttttccc cttaccttat ataacgtaga aagctgaaaa agtacttgtt
    12421 atattgttat tattccttca tactcgtctc tacatagtct aaattcaatt cattaaattc
    12481 ttctaatgta tctaacgctc taacgtattc tatgggggta tttctttctt tgttggataa
    12541 tagcttgttg tattttccca tctgtttgca atttttcgac agcaatgtct catacgatat
    12601 gaaaaactct actagggtgt cttttaccca atattcaaaa ggttacatgt gtttgctgac
    12661 taaaaattat ttccttataa aggatagaat cacaataatt atgcatataa ttccgcctaa
    12721 aagcttgtcc tatttcagtg attttgtcta aaaaattttg cgtatttttt taactgtgtt
    12781 cctttaaaat ttctcatttc tcatgagtct gttcagtttt ttaaagtgta actttgttta
    12841 aaaagctttg caacagaatt actaccagta acactcgtca tataattaat aatttgttgt
    12901 atatattcaa tattaataat aggccattga atacctaata attttagtgt tttttgagtt
    12961 tcagtcgaat caagatttat tggtttataa caattatctt ttggttcttc aaatgtcatg
    13021 aggttttgaa tatcttcctg tttcttttta tctttagaaa gttctaaaat aaattgttga
    13081 tactctgttt cagaaattac tttaattgga tatcccattg aatttagtaa ttttacaagt
    13141 acatttaaat ttaacgtaag atgattgtat atatgattta cgcaattttt gctattttca
    13201 tgtatttcat ttgaagttac aaaccctgtg ataattttgc tgcaataatc tacaggtgta
    13261 aactcaactt cacttgataa taatacatct gtcacgatac catattgtaa tataaatttt
    13321 aatttgctca taaatgcatt ttcggtgaag ttatattgat gttgtccatc aatatatcta
    13381 tttgtaagat ttcctactct ataaatagta gcatctgttc ccgttaatat tgccttgaat
    13441 atttcatatt ccgccaaaaa tttacttttc acatacacat tatctttgta attttgtttt
    13501 acataaaaat cgctttcttt aaatattgtt tccgtttcat ttaatacaaa ttgtccggct
    13561 acaccagttg tagatatatg atgtaaatcc ttagtatcac caacaaatgc aagaagttct
    13621 tttaccgcct ggacatttgc tctttcaaat tcactatact cgccaaaatg ttttgttaaa
    13681 gctgcagcat gaataacagt tgatatttgt tgatgaagct ccttatataa ttcgttttct
    13741 aatcccatat tttgtttagc taaatcacct tgtacaataa agatacgttg atttactaaa
    13801 gattgatagc ttacaaattt atttttaaaa taaaatttta acatgtgtaa taaacgatta
    13861 tttgcctctt gtttattttc cccacgaact aaacaatata tcttacattc tgtagtatcc
    13921 aatagttgtt ctagtaggtg aatacctaag aatcctgtag cacctgttaa aagtattttg
    13981 ctattattac taaattcgtg tctttttgtt ttctttagct tctttggagg agttgtcaat
    14041 ttaatatcta atgtatctga atgtgaattt actttatatc catattgaat ttttttagct
    14101 atattattaa ttgtttttaa ttcaaagtaa tctttaatag ttacattcca accttcagaa
    14161 aatgttttta ctaaagtact cataattttt aaagagtgcc cacctagatc aaaaaaatta
    14221 tcatttatcc caatgacaga ggagtcaatc cctaat
//
