LOCUS       NZ_NUIO01000031        16545 bp    DNA     linear   CON 20-MAY-2024
DEFINITION  Bacillus thuringiensis strain AFS049207
            AFS049207_100_C7_Contig42_140514, whole genome shotgun sequence.
ACCESSION   NZ_NUIO01000031
VERSION     NZ_NUIO01000031.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 16545)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16545)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Assembly Cell v. 4.2.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.45x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002575705.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 01:16:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,769
            CDSs (total)                      :: 5,694
            Genes (coding)                    :: 5,513
            CDSs (with protein)               :: 5,513
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 72 of 182
            Pseudo Genes (incomplete)         :: 113 of 182
            Pseudo Genes (internal stop)      :: 64 of 182
            Pseudo Genes (multiple problems)  :: 54 of 182
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:44:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 16545
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NUIO01000031.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..16545
                     /collected_by="AgBiome"
                     /collection_date="2014-01-14"
                     /country="USA: IA"
                     /db_xref="taxon:1428"
                     /isolation_source="excrement"
                     /mol_type="genomic DNA"
                     /organism="Bacillus thuringiensis"
                     /strain="AFS049207"
                     /submitter_seqid="AFS049207_100_C7_Contig42_140514"
     region          1..16545
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /tool="antismash"
     cand_cluster    1..16545
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..16545
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[6050:13942](+)"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      6051..13942
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     gene            238..693
                     /locus_tag="CN977_RS23130"
                     /old_locus_tag="CN977_23130"
     CDS             238..693
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865202.1"
                     /locus_tag="CN977_RS23130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23130"
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_098829670.1"
                     /transl_table=11
                     /translation="MKTSKVTEWILGGRNPGNYILYNDVNVKYTNEVSKNLYSLENHEG
                     YGTSYCFLDKHLYVGERVKFSGMIMTKSVKEYAGLWIRAEQQGKNLWEVYHIHSNSLSV
                     ENSWKEYFVILDVPKNIETLSVGVSLKGTGHIWFSSMSVEIVDKKNN"
     CDS             complement(879..956)
                     /gene="allorf_00879_00956"
                     /locus_tag="allorf_00879_00956"
                     /protein_id="allorf_00879_00956"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MDTSVFAGISHFKQSVRTVQTVKCK"
     CDS_motif       complement(933..956)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MDTSVFAG"
                     /locus_tag="allorf_00879_00956_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(879..932)
                     /prepeptide="core"
                     /locus_tag="allorf_00879_00956_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-5.60"
                     /molecular_weight="1917.3"
                     /monoisotopic_mass="1916.1"
                     /core_sequence="ISHFKQSVRTVQTVKCK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MDTSVFAG"
                     /leader_location="[932:956](-)"
                     /alternative_weights="1935.3"
                     /alternative_weights="1953.3"
                     /alternative_weights="1971.3"
     CDS             1256..1357
                     /gene="allorf_01256_01357"
                     /locus_tag="allorf_01256_01357"
                     /protein_id="allorf_01256_01357"
                     /tool="antismash"
                     /transl_table=1
                     /translation="MIINPFISFLKEYQASTFLDCILVSHKKSFPVM"
     CDS_motif       1256..1303
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MIINPFISFLKEYQAS"
                     /locus_tag="allorf_01256_01357_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       1304..1357
                     /prepeptide="core"
                     /locus_tag="allorf_01256_01357_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-6.00"
                     /molecular_weight="1911.3"
                     /monoisotopic_mass="1910.0"
                     /core_sequence="TFLDCILVSHKKSFPVM"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MIINPFISFLKEYQAS"
                     /leader_location="[1255:1303](+)"
                     /alternative_weights="1929.3"
                     /alternative_weights="1947.4"
     gene            complement(1436..2314)
                     /locus_tag="CN977_RS30030"
     CDS             complement(1436..2314)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022240.4"
                     /locus_tag="CN977_RS30030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF2599 domain-containing protein"
                     /protein_id="WP_142338658.1"
                     /transl_table=11
                     /translation="MNDRLKKLIISGTLAGIVFSITNVASANSTDISEETTYTNAMNSD
                     YTKDNLTFSTANGEIIKIGHLQSINPTKNNFYFFSNKKDSLPNFKKVKNGVEQSIKIDP
                     TKNNGKYKVPFDFENGESIKVEEDGSAVIYNKNKESIAVVMPTEAKSQSGEILEVHTTL
                     KNENTLEYYVNIKEEVNPITIKNTATSYAFSQYFSSGNWITRDGMVSLSLEPTSVLTKL
                     IVNRAIRTDSWNKVKDTFSMDDQWGNTSSMQNQYLCHYDFAKSFKTPWNLEPHRPDVGY
                     LKTVAATCNPL"
     misc_feature    3062..3075
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 23.97"
                     /tool="antismash"
     gene            complement(3193..3918)
                     /locus_tag="CN977_RS23140"
                     /old_locus_tag="CN977_23140"
     CDS             complement(3193..3918)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465744.1"
                     /locus_tag="CN977_RS23140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23140"
                     /product="ABC transporter permease"
                     /protein_id="WP_098033719.1"
                     /transl_table=11
                     /translation="MILNIKNYASYNLKELFKKGHLAVGLLGALGPALIISYFILKGNT
                     SFHIKHVSNFYCMLGMLAAVLHPLYFVNRDYSSKTISLINNSKQNRKNYVLANVIIALF
                     VSLIYSAIGIVLLLVTKQLGVPGELQASFLLGFFVNVFLLVLTFFLFGYLLLLYGVRSG
                     AVYVILTALLLFVHNILSNLLMSSHNKFLSGLIENFPGYFYPVMVGSNPLSTLQYLIGC
                     LTIIVLFVLVLKKSEKIEM"
     gene            complement(3902..4771)
                     /locus_tag="CN977_RS23145"
                     /old_locus_tag="CN977_23145"
     CDS             complement(3902..4771)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465748.1"
                     /locus_tag="CN977_RS23145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23145"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_098829671.1"
                     /transl_table=11
                     /translation="MHVNSITKKYGGSVALKEISIDFKQGEIIGLVGSNGAGKSTLMKI
                     ITQTIQTFDGSVEGNNSVGYLIEEPKLFSNKTGLEHLSYFSEIYGNTFEISEYEELLKS
                     LQLFNVLNKKVKEYSLGMKQKLGIVISLLNNPNYVVLDEPTNSMDIETSLEVLQQLRKM
                     ANEWNVGILISSHKLEDIETICDRILFLEKGTLLEEQQFNNKNLYILKLVFADSTELAK
                     FIEHQEFGSIIHSSEKTVKIETTVANSEIFQFLNQLGIRLIDFTAEKKTLRNVYMTKIR
                     GEYHDTKY"
     misc_feature    4766..4778
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 21.58"
                     /tool="antismash"
     gene            complement(4798..5151)
                     /locus_tag="CN977_RS30830"
                     /old_locus_tag="CN977_23150"
     CDS             complement(4798..5151)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019257547.1"
                     /locus_tag="CN977_RS30830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23150"
                     /product="DUF2178 domain-containing protein"
                     /protein_id="WP_098829672.1"
                     /transl_table=11
                     /translation="MKIESSSEISNSIFYPLKIWAEKSNGNWNMLIGSGLLLILVGVIL
                     VFVCLKKIGKGDERTNQIYLQSTLIMLSGVILCDLVFPKEYMWQVFFLFKYSLAFLASG
                     IYLAVRYKRDFFN"
     gene            complement(5141..5380)
                     /locus_tag="CN977_RS23155"
                     /old_locus_tag="CN977_23155"
     CDS             complement(5141..5380)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085662.1"
                     /locus_tag="CN977_RS23155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23155"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_075312773.1"
                     /transl_table=11
                     /translation="MKKDFGDSISNKVYEYRVLARMSQQELAEQVGVSKQTIFVMEKGN
                     YAPTLLLAFRIADFYKVDVSDIFTYLKGNDPNEN"
     gene            5580..5768
                     /locus_tag="CN977_RS23160"
                     /old_locus_tag="CN977_23160"
     CDS             5580..5768
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_ha"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF000539.0"
                     /locus_tag="CN977_RS23160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23160"
                     /product="plantaricin C family lantibiotic"
                     /protein_id="WP_218939745.1"
                     /sec_met_domain="mature_ha (E-value: 6.1e-13, bitscore:
                     38.2, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNEISKEVQLAQVTWLEEVVDQEFDDDVFGACTTNTFSLSDYWG
                     NDGSWCTATHECLSWCK"
     CDS_motif       5580..5675
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     MKNEISKEVQLAQVTWLEEVVDQEFDDDVFGA"
                     /locus_tag="CN977_RS23160_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       5676..5768
                     /prepeptide="core"
                     /locus_tag="CN977_RS23160_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="2.00"
                     /molecular_weight="3255.5"
                     /monoisotopic_mass="3253.4"
                     /core_sequence="CTTNTFSLSDYWGNDGSWCTATHECLSWCK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKNEISKEVQLAQVTWLEEVVDQEFDDDVFGA"
                     /leader_location="[5579:5675](+)"
                     /alternative_weights="3273.5"
                     /alternative_weights="3291.6"
                     /alternative_weights="3309.6"
                     /alternative_weights="3327.6"
                     /alternative_weights="3345.6"
     gene            5829..6032
                     /locus_tag="CN977_RS23165"
                     /old_locus_tag="CN977_23165"
     CDS             5829..6032
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF038161.1"
                     /locus_tag="CN977_RS23165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23165"
                     /product="class II lanthipeptide, LchA2/BrtA2 family"
                     /protein_id="WP_098829673.1"
                     /transl_table=11
                     /translation="MNNNKSNTNNSLKNELELGKYLESDMIALTEDDVAGGITPTVSIA
                     LTAVSGYISSNTCPTTACTRAC"
     CDS_motif       5829..5939
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     MNNNKSNTNNSLKNELELGKYLESDMIALTEDDVAGG"
                     /locus_tag="CN977_RS23165_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       5940..6032
                     /prepeptide="core"
                     /locus_tag="CN977_RS23165_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-0.90"
                     /molecular_weight="2805.2"
                     /monoisotopic_mass="2803.5"
                     /core_sequence="ITPTVSIALTAVSGYISSNTCPTTACTRAC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MNNNKSNTNNSLKNELELGKYLESDMIALTEDDVAGG"
                     /leader_location="[5828:5939](+)"
                     /alternative_weights="2823.2"
                     /alternative_weights="2841.2"
                     /alternative_weights="2859.3"
                     /alternative_weights="2877.3"
                     /alternative_weights="2895.3"
                     /alternative_weights="2913.3"
                     /alternative_weights="2931.3"
                     /alternative_weights="2949.4"
     gene            6051..8984
                     /gene="lanM"
                     /locus_tag="CN977_RS23170"
                     /old_locus_tag="CN977_23170"
     CDS             6051..8984
                     /GO_process="GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="lanM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03897.1"
                     /locus_tag="CN977_RS23170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23170"
                     /product="type 2 lanthipeptide synthetase LanM"
                     /protein_id="WP_098829674.1"
                     /sec_met_domain="DUF4135 (E-value: 1.1e-65, bitscore:
                     212.1, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 1e-48, bitscore:
                     155.7, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVTYRKQFYPELEQSDFDEHIAPLLHYIDDLSVHPTNNLNNAKTF
                     KDQELYPYHNSCRMISRNIWEKVWDPKMDKYLQYPESFKNVICELFSQIAFSYLIRCFA
                     EQIKTLPKQSMNSKDIYDYYNQKLMDTQFKEFADIYSVVWGRCNNLLKNRAIAIKETIQ
                     LTQKHRLKIEEEFNIPCTSRIVSVESGGDTHNNGSSVAIITFEQGEKLVFKPRSVSGEL
                     GYTNFIREINKFISPKMPSLTVIDFGNYGFTSFVETNEEKNDMVQAGRLACLMFLLNAS
                     DMHYSNILWTNEGPLPIDLETLFHPSRIRTGIPESKRSAYHAMEKSVYGTGVLPISLGK
                     KTSNDSVDVGFTGIRNQNSVSPFKVFEIKDGFSSDIKVVWKRQSIDISLSNDPNLESLI
                     HKRCEQVIDGFSEFFMQILHKKDLFVDAVIKSFSNAKFRYIHNMTYRYSQLLRILTDAE
                     PSRNIETAHALLSRIGILSMSSDVNLVLSECKQLWNGDIPYFSVNFKGTEVLCGNEVVS
                     SISTSPESQFISKMKNLTKTDMTKQINLIRLAFVAKLADPHADGKLKLEESESIEEDRP
                     RFERMNTAKDNNILKHREIISWFSKSLTESLLDDRYEHLPKTWIGPVAKNGTQGWAPGV
                     LGYDLYSGRVGPALALAVSGKLLSDDRAIKASYEVFEKSAKILEERSFELRNVLLSGIG
                     GFSGVSGLLWTLYAAGDIVENNRWKDIAVNSWPILHRSLESQDNNFFDMITGKSSSIVM
                     RYRMQKDFKLSDDIINKILSLAYSRINANDTKITSGLTHGYGQLLWFFALVNQKQENSA
                     VKRVVEDIDSIICNKYTNNDGITETYIDGGSTSISSSWCNGLAGLLIAYYEAYKANILP
                     QESVLNIINQLKRIPISRVPVLCHGSLGIVEALQYVSESFSEETSDILLEMQTTFCSPE
                     YIFSYYKNGKGRYPLSPGLMAGQAGALLHLCKSLDSTIKTSPLTFDY"
     gene            8986..11163
                     /locus_tag="CN977_RS23175"
                     /old_locus_tag="CN977_23175"
     CDS             8986..11163
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615515.1"
                     /locus_tag="CN977_RS23175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23175"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_098829678.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.6e-35, bitscore:
                     110.0, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKILSKIKKRKLTPTLQLTQAECGLCCVRTILEAYDYQISMTELR
                     QVKEPGRDGLGLQQLKSLLSHFGMDAKTYRIKDTRALTVIDLPIIVFWKGSHFVCVESY
                     NEHEVIIMDPSIGRITISTSDFSENSDGYILVAQPGIDFKRRSVSKLSMWKKTYIWPSN
                     MIGLYLKIALISIVLVGMTLAIPIFTQQLIDNGLGDAISFASILGSLIIALIIMISLNF
                     LRTFFSIKIIYRFSWYLVSGAFTRLLSLPAKYFTVRAPGEIIYRLNSLTRIQGVLGTTL
                     VQTCLDFISGVAILGYVFWISPLLGVMILLSILSAFALLIIGQSYVSSATDKELHEATN
                     AQSIQLDAVISINSVKLGGYVQSYINDWEQRFKKFLNATSHRMRIQQGIIGSILAGIQV
                     FAPLIILVTSLYLAESGLVTLGQAIAIQSIASLLFVYVNSIFTVVSEVVVAARYIVLAE
                     DIFEYPIEYSRKDSKGMPTGSISIKNLNFSYTSDGIPSLKNINMDISDGETIAIVGVSG
                     SGKTTLGKVISSLFEPTSGSMYFGGIEYHEYNLDALRDSISYIPQEAHLHNRTIMENLK
                     LGTKLTETEIIEFCDSLEFLDFINKFPMGYNTVISETGANLSGGQRQRIHIARVLLQTP
                     KLLIMDEATSSLDNISQSYVYDTLSKLKCTKVVIAHRFATILNADRIFVLENGCIVQSG
                     SHEELINKDGLYAKLFGAELERRSEYATSSR"
     gene            11144..13942
                     /gene="lanM"
                     /locus_tag="CN977_RS23180"
                     /old_locus_tag="CN977_23180"
     CDS             11144..13942
                     /GO_process="GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="lanM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070: lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03897.1"
                     /locus_tag="CN977_RS23180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23180"
                     /product="type 2 lanthipeptide synthetase LanM"
                     /protein_id="WP_180232510.1"
                     /sec_met_domain="DUF4135 (E-value: 9.9e-77, bitscore:
                     248.4, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 1.2e-35, bitscore:
                     112.8, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQQVADKLVHAVESFFSDEIQHTNYKIKELLSQQEVVSNAEIASM
                     NCLHALLEYHVWRLCHKTFIYEFHEYRESLSYPADPLSSKIFDQYVSSIDKQLISSWFT
                     KYDVLRKMVTQATNNTCSFIEDMCANFSSDATLLVSRGLISRGARLQAIRPLDSDPHNG
                     SKVVLCLEFESSKKILYKPRSLEIDVLIDKLFSETLKFDTLQDYSPVPRTVNRGEYGWQ
                     EFVQHTPISQSEIGSAYYNLGLCAAVFSCLGATDLHDENIIFNGIFPYVIDLETSIQPT
                     YSRTGNNLHELMGERIAHSIANTSIIPAKQVTIPHEILIGAINTPYPQTTNEMVFSLKN
                     PGTDAIDLAKEKITVTRTTVPIMLPGNQAPDPLPYQEDFLKGYSDGYEKMLDKREQIYS
                     ILTDIQSLIRLVIRPTVQYYFMLDACLFPENLVNDNAINQVLKYLKPPKLIRDSEIAKN
                     TLEEEIRSIKNGDIPHFSIKANGKRLYSGDFISNEIFDVSPANHAMEKLKDLSQKRLLH
                     DKRLIAEGYSEIRIHEAEHKKVDDLGYQSPLFSNVLRKVTKDNPNPLIELISSLSITTK
                     TDNAETGWIGGVYGNIPVSYQSTALISLHDTGGILFLLEHLTEDKNTANRSEYTNLFNQ
                     AKRGLKSLRSAFSSQLESAPTSIISGSSSLDFIFNHNTNRVKEIEQIVSQIQSENIPRG
                     DVFIGPVGIGLILASFSETPNQILKNLEQKICDESPSNMLSSDGIAHGTLGVIWAQFRL
                     SYALKNTEKCKYLFKKASQISFPNVVNTAGWCNGNAGLLMVLAEMARALNEPLDFYELA
                     KKSTALPKNGPIDLSICHGAAGVLQSLLFTYEASGDYWYLSLANQYWETVLDLANNHGF
                     YTGEKNRDYLLGYFLGWSGVADSALLLKMYNNGDSAWFPLNLSSVSYQRNNIYKEEKEC
                     VH"
     gene            13930..15024
                     /locus_tag="CN977_RS23185"
                     /old_locus_tag="CN977_23185"
     CDS             13930..15024
                     /NRPS_PKS="Domain: PKS_ER (44-206). E-value: 2.3e-14.
                     Score: 45.2. Matches aSDomain:
                     nrpspksdomains_CN977_RS23185_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /locus_tag="CN977_RS23185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23185"
                     /product="alcohol dehydrogenase catalytic domain-containing
                     protein"
                     /protein_id="WP_098829676.1"
                     /transl_table=11
                     /translation="MRSLVAINPSKKNSMDYTELGIVKFGQEDILFGTVELEQPFFDTE
                     DPKNREYVLVRVTAFSCNYRDKALLLNNYNSIKGSDRLFVPFGSEFSANIVAIGKDVHE
                     FKVGDSVMSDCSYPDSGKQGVLPGIATNFASLGWLRLHKKKIIKKPKSLSDSEAACFSL
                     GSQTASGMIRRSGILENGGNPLVFSARSSTSLFIIQQLLSYGITPICLSTSEWSDEEIQ
                     KIYPSRVELVSNAMNGASSDIVKQKITHVFDPFFDMNIGAALYYLQTGGTYVTCGLRDQ
                     HPLLSNDTPSDAEPMVRGALQMSIFKNVSILGNCLGSKEDLEKAIQLQNKIIIDQEYQL
                     EKGVDFVQRSFFDSSRFGKCVLFL"
     aSDomain        14062..14547
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CN977_RS23185_PKS_ER.1"
                     /evalue="2.30E-14"
                     /label="CN977_RS23185_PKS_ER.1"
                     /locus_tag="CN977_RS23185"
                     /protein_end="206"
                     /protein_start="44"
                     /score="45.2"
                     /tool="antismash"
                     /translation="EDPKNREYVLVRVTAFSCNYRDKALLLNNYNSIKGSDRLFVPFGS
                     EFSANIVAIGKDVHEFKVGDSVMSDCSYPDSGKQGVLPGIATNFASLGWLRLHKKKIIK
                     KPKSLSDSEAACFSLGSQTASGMIRRSGILENGGNPLVFSARSSTSLFIIQQLLSYGI"
     gene            15609..>16072
                     /locus_tag="CN977_RS23190"
                     /old_locus_tag="CN977_23190"
                     /pseudo=""
     CDS             15609..>16072
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611090.1"
                     /locus_tag="CN977_RS23190"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="CN977_23190"
                     /product="IS3 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKTRVHYPEEVKWKVIEMKKDGYSNRTIMEKLGIKNVSQIKTWMK
                     "
     gene            16371..>16545
                     /locus_tag="CN977_RS23195"
                     /old_locus_tag="CN977_23195"
     CDS             16371..>16545
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016082930.1"
                     /locus_tag="CN977_RS23195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_23195"
                     /product="replication initiation protein"
                     /protein_id="WP_142338659.1"
                     /transl_table=11
                     /translation="MSLQQELQIKENNIVSKSNTLIEASSRLNLVEQKMLLCLASNIEP
                     SDRDFKTYTFPIK"
ORIGIN
        1 tgttatggat agtatttatg aatacataaa aaagaataga agaaaaggaa gtcccagata
       61 atcttttggg gcttatttgt tattaataaa tcaacgactg ctatgcttaa atatagaagt
      121 tgttggttgt gatggcattt agcaaaaatt atcaaaaaaa acatagtagt tatgagtata
      181 attcaaatta ggttaagttg attactgaaa ttaaataaat gaataggcgg tataataatg
      241 aagactagta aagtgacaga atggatatta ggtggacgga atccgggtaa ttatatttta
      301 tataatgatg ttaatgtgaa atatacaaat gaagtttcaa aaaacttata ttctttagaa
      361 aatcacgaag gatatggtac atcatactgt tttttggata aacatctata tgtaggtgaa
      421 agggttaaat tttcagggat gataatgaca aagtcagtta aagaatatgc gggtttatgg
      481 attagggctg agcaacaggg aaaaaatctc tgggaggttt atcatataca ttctaattcc
      541 ttaagtgtgg aaaattcgtg gaaagaatat tttgtgattt tagatgtacc taaaaatata
      601 gaaacattat cagtaggagt gtctttaaaa ggtactggtc atatatggtt tagttcaatg
      661 agtgttgaaa ttgtagataa aaagaacaat taatgtagat ttaaaataaa atggaatcaa
      721 aaattccccc tcaaatgttt cttacaaact tttgagaata cccctgttag tccctacatt
      781 gatatagtaa aagaagagga gtgaggacac atgcgtgaaa ttccttggcc agattcagat
      841 tcttttctag ggtttatagg taattaacaa atagatgatt atttacattt aacagtttga
      901 accgtcctta cggactgttt gaagtgggag attcctgcga acactgatgt atccaccggt
      961 taccttagca ggctctgtcc gtagtcccta cggtgaaaac atcagtatct tgtacgttga
     1021 ctcaccacgc ttcctacttt agctttgttt tcgcaacttc agcaaacgtg gggcgaaatc
     1081 tacttgaagt aacaatgact aagtaagaaa aagaaaggca gataaggaat atgaaaaagt
     1141 gagcatttat tcaagcagtt taagtatcca aacgggggga atcctctttt ttatataaaa
     1201 tttgcaattt catatacttt tttcaattag ttgaatttga acaatatcac tatagatgat
     1261 aataaatccc ttcatttcct tcttaaaaga atatcaggct tcgacttttc tggattgtat
     1321 acttgtgtcc cacaaaaaaa gctttccagt tatgtaactt ggaaagctct ttatatggtg
     1381 acccctacta gatagttaat cttctagata gttaaccttc tagatagtta tgggtttata
     1441 acggattgca tgtagcagca acagtcttca aatagccgac atctggacga tgcggttcta
     1501 agttccatgg tgtcttaaat gattttgcaa aatcataatg acataaatat tgattttgca
     1561 tagagcttgt atttccccat tgatcatcca tcgagaaagt atccttaact ttattccaac
     1621 tgtctgttcg aattgcgcgg tttacaatta attttgttaa tacacttgtg ggctctaagc
     1681 ttaaagaaac cataccatct cttgtaatcc agttaccact agaaaaatat tgtgagaaag
     1741 catatgatgt ggctgtgttc ttaattgtta tgggattaac ttcttcttta atatttacat
     1801 aatactctaa ggtgttctcg tttttaagtg tagtatgtac ttctaaaatt tctccagatt
     1861 gagatttagc ctcagtgggc ataacaacgg caattgattc tttattttta ttgtaaataa
     1921 ctgcagaacc atcttcctct acctttatcg attcgccgtt ttcgaaatcg aatggaactt
     1981 tatattttcc attatttttt gtaggatcga tttttataga ttgttcaact ccatttttca
     2041 cctttttaaa attaggtaat gaatctttct tatttgaaaa aaaataaaaa ttattttttg
     2101 taggattaat tgattgtaga tggccaattt ttataatttc tccattagca gtagaaaaag
     2161 ttaaattatc tttagtataa tcactattca tcgcatttgt atatgttgtt tcttctgata
     2221 tatctgtgga atttgcagaa gctacatttg taatactaaa tacgattcca gctagggtcc
     2281 ccgatataat taatttcttc agtctatcat tcaattacaa catctcctct gatttttatt
     2341 atcacaactt tctacaaatt aacatatata gatatgaatg taaatgtttc tttttgtttt
     2401 ttatgaaaaa atagaaaatt tacatattta ttacaactcc ttcaattgag gaaaaaagat
     2461 aggaggtttt ttattatttt aagacttcgg tacagaaaac ttaaaatata tggaaataga
     2521 attctactgt aaaacccctt gtaaactgaa tattaagaaa ttttataaaa ggaatcataa
     2581 atgggataat taaaggaaag caatttaaac aagatatttc tttgatagcc ttggctacta
     2641 tgtatctttg tttttagtct atcttgatac ctctgaaatt ttaagagaat gtgatttttc
     2701 agttcaatta aaatgtcccc ccgtttactt aaggggagac gtttgaaagg ttagtaggat
     2761 catctttact atcgtaacca gattcaatct caattacata ttgttgcttc attaacacaa
     2821 cactcctttt atgataaaaa agggattgat taatttaagt gtagatctcc gtaactttta
     2881 tacttcagct agttgcataa caatgagctt aatcagtata tacaatttat aaaagggtat
     2941 gcttgttttt gcataccctt tcttgtattt taatgaagaa atatagctta atattatcta
     3001 ttaagtaaat aatcccaagc ttttttaata actcctggaa atggcataaa acacctcttt
     3061 atataattat tactatatta tattgaaatt gatataacat ataaaaatat aacattttac
     3121 ggtaatgtct aattttagtt atatacagga atctatcata tacaacgcgt aataaaatcc
     3181 aataaaacct tcctacattt caatcttttc gctcttttta agaacaagta caaaaagtac
     3241 gattatggtt aaacaaccaa taaggtattg tagagtggaa agtgggtttg aaccaaccat
     3301 aactggatag aaataaccag gaaaattttc gattaagcct gaaagaaatt tgttatgact
     3361 actcatcaat aaatttgaca aaatattatg aacgaaaagt aacaaagctg ttaaaattac
     3421 ataaactgca ccactccgaa ccccatataa taataataga tatccgaaca agaaaaaagt
     3481 caacactaga agaaagacat taacaaagaa acctagaagg aaggaagcct gcaattctcc
     3541 aggcactcca agttgttttg taactagaag caaaactatt cctattgctg aatagattaa
     3601 actcacaaat agtgcaataa tgacattagc tagcacatag ttttttctgt tttgttttga
     3661 gttgttaatc aatgagattg tcttagatga gtaatctctg ttaacaaagt aaagtgggtg
     3721 taatactgcc gcgagcattc ctaacataca ataaaaattt gagacatgtt taatgtgaaa
     3781 cgacgtgttt ccttttagga taaagtagct tatgattagt gctggcccta aggctcccag
     3841 caatcccaca gccaaatggc cttttttaaa tagttctttt aaattgtaac tagcataatt
     3901 tttaatattt agtatcatga tattcacctc tgattttggt catataaaca ttacgaagcg
     3961 ttttcttttc agcagtaaaa tcaattaatc ttattcctaa ttgatttaaa aactggaaaa
     4021 tctcagaatt agctactgtg gtttcaattt taactgtttt ttcacttgaa tggatgatac
     4081 tcccgaactc ttgatgttca ataaattttg ctaattcagt ggaatcagca aaaactaatt
     4141 ttagaatata cagattcttg ttgttaaatt gttgttcttc aagaagagtg cctttttcta
     4201 aaaacaagat gcgatcacat atagtttcaa tatcttctaa cttatggctg gaaattaaga
     4261 taccgacatt ccactcatta gccatttttc tcaattgctg taatacttcg agggatgttt
     4321 ctatatccat gctgtttgtt ggttcatcca atacaacgta atttggatta ttcaatagac
     4381 ttataacaat tcctaatttc tgcttcattc ccaaagaata ttcttttact tttttattca
     4441 atacattaaa taattgtaga ctttttagaa gttcctcgta ttcactaatt tcgaatgtat
     4501 ttccatatat ttctgagaaa taggacaaat gctctaatcc agtcttatta ctgaagagtt
     4561 tgggttcttc aattaagtat ccgacagaat tattgccttc aacagaccca tcaaatgtct
     4621 gaattgtttg agtgattatc ttcataagtg tactttttcc agcaccattg cttcctacta
     4681 aaccaatgat ttccccttgt ttgaaatcaa tagatatttc ttttaaagct acagagccac
     4741 catatttctt agtaattgaa tttacatgca ttttccaata tcctcctgtt tatttttcta
     4801 gttaaaaaaa tctcttttat atcgaacagc tagatatatt cctgaagcga ggaacgctag
     4861 agaatatttg aataagaaga aaacttgcca catatattct ttcgggaaaa ccaagtcgca
     4921 caaaattact ccacttaaca ttatgagggt actttgtaaa tatatttgat ttgttctttc
     4981 gtcaccttta cctatttttt ttagacatac gaataccagt attaccccca ctaaaatcaa
     5041 tagcaaaccg ctaccaataa gcatattcca attaccattt gatttttccg cccagatttt
     5101 taaaggataa aagattgaat tcgatatttc tgagctagat tcaattttca tttggatcat
     5161 ttcctttcaa ataagtaaaa atatcactga catcaacttt ataaaaatcg gcaatacgaa
     5221 aagctagtag taacgtaggc gcataatttc ctttttccat tacaaaaatt gtttgtttag
     5281 aaacaccaac ttgctctgcc aactcttgct gagacattct ggcaagtact cgatattcat
     5341 agactttatt tgatatcgaa tcgccaaaat cttttttcat aatcatcacc tcttaaaaag
     5401 agtgtaaatt accattttat aaaagtaaag taaactttta taaaataagg atattgttat
     5461 atatttaaaa aaaattgtaa attattaacg attttgcttg cctaaatata tattattata
     5521 ttaatctgta aattgtaata cttcaaaaaa atgtttattt ttcaaggagg tggaataaag
     5581 tgaagaatga aattagtaaa gaggtgcaac ttgcacaagt tacttggtta gaagaagttg
     5641 tagatcaaga atttgatgat gatgtatttg gagcatgcac tactaataca ttttctctta
     5701 gtgattactg gggaaatgat gggtcgtggt gtacagctac tcatgaatgc ttgtcatggt
     5761 gtaaatagtt tagaaatgct tctatggaat aataattaaa aaataattta tgaactgggg
     5821 agctaataat gaacaataat aagtctaata ctaataatag tttaaaaaat gaattagaac
     5881 ttggaaaata tttagaatca gatatgattg cattaactga ggatgacgtt gctggcggaa
     5941 ttaccccaac agtatcaatt gctcttacgg cagtttcggg atatatttca agtaatactt
     6001 gtccgactac tgcttgtaca cgcgcttgtt aaaggaggga tagaaaattt atggttactt
     6061 ataggaaaca gttttatccg gaacttgaac agtcggattt cgatgaacat attgcaccac
     6121 ttctccatta tattgatgat ttatctgtgc accctacaaa taatttgaat aatgcgaaaa
     6181 cctttaagga ccaggaatta tatccctatc ataactcttg taggatgatt tctagaaaca
     6241 tctgggagaa agtatgggac ccgaagatgg acaagtatct tcagtatccg gaatctttca
     6301 aaaacgttat ctgtgagcta ttctcacaga tagcgttttc atatttaata agatgttttg
     6361 ctgaacagat taaaacatta cctaagcagt ctatgaattc aaaagatatt tatgattact
     6421 ataatcaaaa attaatggat acacagttta aagaatttgc ggatatttac tcagttgtgt
     6481 ggggaagatg taataatcta ttaaaaaata gagcaattgc aatcaaagag actattcagt
     6541 tgactcaaaa acatcgtttg aaaatagagg aagaattcaa cattccgtgc actagtcgta
     6601 ttgtttctgt agaatcagga ggagacacac ataataatgg gtcttcagta gcgattatta
     6661 cctttgaaca aggagaaaag cttgttttta aaccacgttc agtctctgga gaacttggat
     6721 atacaaattt tattcgagag attaataaat ttatatctcc aaagatgcct tctctgactg
     6781 ttattgattt tggtaattat ggttttacgt cgtttgtaga aactaatgaa gaaaagaatg
     6841 atatggttca agccggcagg ttagcatgtt taatgttttt attaaatgct tcagatatgc
     6901 attatagtaa catattatgg acaaatgagg ggccacttcc aatagatttg gaaactctgt
     6961 ttcatccatc tcgcattaga actggaattc ctgagtctaa aagaagcgcg tatcatgcaa
     7021 tggagaaatc ggtatatggt acaggtgtac ttccaatatc attaggtaaa aaaacaagta
     7081 atgattcagt tgatgtcgga ttcactggca ttcgtaatca gaatagtgta agtcctttta
     7141 aagtcttcga aataaaagat ggtttttctt cagacataaa agttgtctgg aagcgtcaaa
     7201 gtatagacat ttctttatct aatgatccca atttagaatc gttaatacat aaaagatgtg
     7261 aacaagttat tgatggtttc tctgaatttt ttatgcaaat attgcataaa aaagatttgt
     7321 ttgttgatgc agttataaag tcatttagta atgctaaatt tcgctatata cataacatga
     7381 catatagata ttcacaacta ttacgtatct taacagatgc ggaaccatcc aggaatatag
     7441 aaacagcgca tgctttattg tcacgcattg ggatcctgag tatgtcaagt gatgtcaatt
     7501 tggttctatc tgaatgtaaa caactttgga atggggatat accgtacttt tccgtcaatt
     7561 ttaaaggaac agaggtttta tgtggaaatg aagttgtttc ttctatttca acaagtcccg
     7621 aatcacaatt tatttcgaaa atgaaaaatc ttacaaaaac agacatgact aaacaaatca
     7681 atctaatacg attagctttt gttgctaagt tagcagatcc acatgctgat ggaaagttga
     7741 aattagagga atcagagtct atcgaggaag acagacctag atttgaaaga atgaatacag
     7801 caaaagataa taatatttta aagcataggg aaataatttc ttggttttct aaatcattaa
     7861 ctgagtcact tttggatgat cgctacgaac atttacctaa aacttggatt ggtcctgttg
     7921 caaaaaatgg gactcaaggt tgggcaccag gagttttagg atacgattta tatagcggca
     7981 gagttggccc agcacttgca ttagcagtgt ccggtaaact tttatcggat gatagggcaa
     8041 taaaagcctc ttatgaggtt tttgaaaaat cagcgaaaat tctagaagaa agaagtttcg
     8101 aacttagaaa tgtccttctt tcaggaattg gtgggttttc tggagtttca ggtctactgt
     8161 ggacattata tgcggcaggg gatatcgttg aaaacaacag atggaaagac atagctgtta
     8221 attcttggcc gatattgcac cgttctttgg aatcacaaga taataatttt ttcgatatga
     8281 ttacggggaa aagcagttcg attgttatga ggtaccgaat gcaaaaagat tttaaattaa
     8341 gtgatgatat tataaataaa attttatcat tggcatattc aagaataaat gcaaatgata
     8401 caaaaataac ttcaggattg actcatggat atggtcaatt actttggttt tttgctttgg
     8461 tcaatcaaaa acaagaaaat agcgcggtaa aaagagtagt tgaagatatt gattcgatta
     8521 tctgtaataa atatacaaat aatgatggaa tcacagagac atatatagat ggcggaagta
     8581 cgagtatatc atcatcttgg tgcaatggtt tagcaggact attaatagcc tattacgaag
     8641 catacaaagc aaacattttg cctcaagaat cagtactaaa tataattaat cagctaaaaa
     8701 ggatacctat ctcacgggtt ccggtacttt gtcacggaag cttgggaata gtagaagcgc
     8761 ttcagtatgt aagtgaatca ttttcagaag aaacatctga cattcttcta gaaatgcaaa
     8821 caactttctg ctctccagaa tatattttta gttactataa aaatgggaaa ggccgatatc
     8881 ctctaagccc aggattgatg gcgggtcaag ctggtgcgct attgcacctt tgcaaatctc
     8941 ttgattctac tattaagact tcccctttaa cgtttgatta ttaacatgaa aatactttct
     9001 aaaattaaaa aaagaaaact cacgccaact ttacaattaa cacaagcaga atgtgggcta
     9061 tgctgtgtta gaacaattct ggaggcttat gattaccaaa tttcaatgac agagctcaga
     9121 caagttaaag agccaggtag agacggttta ggattgcaac aactaaagag tttgctctct
     9181 cattttggga tggatgcaaa aacttatcga ataaaagaca cgagggcact tacagtaata
     9241 gaccttccaa tcatagtttt ttggaaaggc tctcattttg tttgtgttga atcatataat
     9301 gaacatgaag taataatcat ggacccttcg ataggtcgga taacaatatc cacatcagat
     9361 ttttcagaaa actccgatgg gtatatacta gttgctcaac ctggaataga ttttaagaga
     9421 cgcagcgtta gcaaactttc tatgtggaaa aaaacgtata tttggccatc taatatgata
     9481 ggtttatatt taaaaatagc tttaataagt attgttttag ttggaatgac attagcaatt
     9541 cccatattta cacagcaatt aattgacaat gggcttggtg atgctatttc ctttgcttca
     9601 atactcgggt ctctgatcat agcattaatt atcatgatat cgctaaattt tctcagaacc
     9661 ttcttctcaa taaagataat ttatcgtttt agctggtatc ttgtgagtgg tgcttttaca
     9721 cgtcttttat cactcccagc aaagtatttt acagtaaggg cgccaggtga aattatttat
     9781 cggcttaact cccttacacg aatacagggt gttttgggca caacattagt acagacatgt
     9841 cttgatttta taagcggtgt agcaatatta ggctatgtgt tttggatatc cccattactc
     9901 ggagtaatga tactgttgtc cattttatca gcttttgctt tattaattat tggccaatct
     9961 tacgtgagtt ctgcaacaga taaagaacta catgaggcaa ctaacgcaca aagtatacag
    10021 ttagatgcgg tgatttccat aaatagcgtg aaattaggag gctatgtcca gtcctatatc
    10081 aatgactggg aacagcgttt caaaaaattc ttaaatgcaa caagccacag aatgcgtatc
    10141 caacaaggaa taataggatc gatattagcc ggaatacaag tatttgctcc tttgattatt
    10201 cttgttacaa gtttatatct ggcagaatca ggactcgtaa cactcgggca agcaattgcg
    10261 attcagtcaa ttgcgtcatt attattcgtt tatgtgaatt caatatttac agttgtatca
    10321 gaagttgtgg tagcagctcg atatattgtg ttagctgagg atatatttga atatcctata
    10381 gaatactccc gtaaagattc aaagggaatg cctacaggat caatatctat taaaaatctt
    10441 aattttagtt atacttccga tggtattccg tcccttaaaa acattaatat ggacatttct
    10501 gatggagaaa cgattgctat tgttggggta tccggatccg ggaagactac gcttgggaag
    10561 gtaataagta gtttatttga acctactagc ggttctatgt attttggggg aattgagtat
    10621 catgaatata atcttgatgc attaagagac tcaattagct atattcccca agaagcacac
    10681 ttgcacaatc gaactataat ggaaaacctc aaacttggca ctaagctaac agaaacagaa
    10741 ataatagagt tttgtgattc attagagttt ttggatttca ttaataaatt tcctatgggg
    10801 tacaatacag tcatatcaga aacgggagca aacctatctg gaggacagag acaacgtatt
    10861 catatagcaa gggttttact tcaaacaccc aaattactga taatggatga agcaacttca
    10921 tcacttgata atatttcaca atcatatgtt tatgatacat tatcaaaact taagtgtaca
    10981 aaagttgtta tagcacatcg gtttgcgact attctaaatg ctgatcgtat ttttgttctt
    11041 gaaaatggat gcatcgtaca aagtggatct catgaggaat taattaataa agatggatta
    11101 tatgcaaaat tgtttggtgc ggagcttgaa aggagaagtg aatatgcaac aagtagcaga
    11161 taaactagtc catgctgtgg agagtttctt ttcggatgaa atacaacata ctaattataa
    11221 gataaaagaa cttctttctc agcaagaagt tgtatcaaat gctgaaatag catctatgaa
    11281 ctgtttacat gcattattgg aatatcatgt gtggagactg tgtcataaaa ctttcatata
    11341 tgaatttcac gaatatcgag aaagtttatc ttatcctgct gatcctttat catcaaaaat
    11401 atttgatcaa tatgtcagct caatcgataa gcaactcata agcagttggt ttacaaaata
    11461 tgatgtttta agaaaaatgg tcacacaagc aacgaataat acttgttctt tcatcgaaga
    11521 tatgtgcgct aattttagca gtgatgccac tttacttgta tcaagaggtt taattagtag
    11581 aggagcaaga ttacaagcta tcaggcctct tgactccgat cctcacaacg gttcaaaagt
    11641 tgtcctttgt ttggaatttg aatcgtcaaa aaagattcta tataagcctc gctctttaga
    11701 gattgatgtt ttaatagata agcttttttc tgaaactctg aaatttgata ctttacagga
    11761 ctattcacca gttcctcgaa cggttaatag gggggaatac ggttggcaag agtttgtgca
    11821 gcatacacct ataagccaat cagagattgg aagtgcttat tataatctcg gtttatgtgc
    11881 ggcggtgttt agctgtcttg gagcaacaga tttacatgat gaaaatatta tttttaatgg
    11941 gatctttcct tatgttatag atttagaaac cagtatacaa ccgacataca gtcgtacagg
    12001 taataacctc cacgaattaa tgggggaaag aattgctcat tcgattgcta atacgtcaat
    12061 aattccagct aaacaagtga caattccgca tgagatattg attggcgcta ttaatacacc
    12121 ctatccacaa acaaccaatg aaatggtttt ttcattgaaa aatccaggga cagacgctat
    12181 agatttggcc aaggaaaaga ttactgttac tcgaacaact gtaccaatca tgttacctgg
    12241 taatcaagca cccgatccgc ttccatatca agaagatttt ctcaagggtt actcagatgg
    12301 gtatgaaaaa atgttagaca aacgagagca aatttattct atattaactg atattcaatc
    12361 tttaatcaga ttagttataa ggcctacagt tcagtattac tttatgcttg acgcatgtct
    12421 attcccagaa aatttagtta atgataatgc aataaatcaa gtgctaaaat atctaaaacc
    12481 tcctaagtta attagggatt ctgaaatagc taagaatacg ttagaagaag aaattcgttc
    12541 tataaaaaac ggggatattc cacacttttc cataaaagct aatggtaaac gtctgtattc
    12601 aggggacttt atttcaaatg agatatttga tgtatcaccg gcaaatcatg caatggaaaa
    12661 gttaaaggac ctttcacaaa agcggctatt acatgataag cgcttaattg cagaaggata
    12721 ctctgagatt agaatacatg aagcagagca taaaaaagta gacgatttgg gatatcaatc
    12781 ccctctcttt tcaaatgtct tgcgaaaggt gacaaaagat aatcctaatc ctttgattga
    12841 attaataagt tcattgtcaa ttactaccaa aacagataat gctgaaactg gatggattgg
    12901 aggagtatat ggaaatatcc ctgtatccta tcaatctact gctcttattt cgcttcatga
    12961 tactgggggt attctatttc tcttggagca cctgacagaa gataaaaaca cagcaaatcg
    13021 ttccgagtac actaatcttt ttaatcaggc taaacgaggt ttaaaatctc ttcgtagtgc
    13081 attttcttca caattagaat ctgcaccaac atcaataatt tcaggatcta gttccttgga
    13141 ttttatattt aaccataata cgaatagagt aaaagaaata gaacaaattg tttctcaaat
    13201 tcaatcagag aatattccgc gtggtgatgt atttattggt ccagtaggta taggtctcat
    13261 attagcatca ttttcggaaa cgccgaatca gattttaaaa aatttagaac aaaaaatttg
    13321 cgatgaaagc ccatcgaata tgttatctag tgatggaata gcacatggca ctttaggagt
    13381 tatatgggca caatttagat tatcttatgc tttaaaaaat actgaaaaat gtaagtatct
    13441 ttttaaaaaa gcttcacaaa tatcttttcc gaatgttgtt aatactgcag gttggtgtaa
    13501 cggaaatgct ggcttgttaa tggttctagc tgaaatggca agagcattaa acgaaccatt
    13561 agatttctat gaattagcga agaaatcaac agcattgccc aaaaatggac ctatagattt
    13621 atctatatgt catggggctg ctggggtact tcaatctcta ctatttacat atgaagcttc
    13681 tggggattat tggtatctat cacttgcaaa tcagtattgg gaaactgtat tagatttagc
    13741 taataatcat ggattttata ctggagaaaa gaatagagac taccttttgg gatactttct
    13801 tggttggagt ggagtagctg attctgctct cctgttaaag atgtacaaca atggtgactc
    13861 tgcttggttt ccacttaatc taagctctgt ttcatatcaa cgaaataata tatataagga
    13921 ggagaaagag tgcgttcatt agtggcaatt aatccgagta agaagaattc aatggattat
    13981 acagaactag gaattgttaa gtttggacaa gaagatatac tgtttggaac agttgaatta
    14041 gaacaacctt ttttcgatac tgaagatccg aagaatcgtg aatatgtatt agtaagagta
    14101 acagcttttt catgcaatta ccgtgataaa gccttattac ttaataacta caattcaatc
    14161 aagggttctg accgattgtt tgttcctttt ggctcagaat ttagcgcaaa tattgttgct
    14221 atcggtaagg acgtacatga atttaaggtc ggggatagtg ttatgtctga ttgttcatac
    14281 cctgatagcg gaaaacaagg agttttaccc ggtatagcta caaattttgc atcgttgggt
    14341 tggttgcgtc tccataagaa aaagattata aaaaaaccaa aaagcctaag tgatagtgaa
    14401 gctgcttgtt tttctttggg atcccaaacc gcgtcaggga tgataagacg ctctggaata
    14461 cttgagaatg gtgggaatcc attagtattt agtgcacgat caagcacatc actgtttatt
    14521 attcaacagt tactctctta tggcatcact ccaatttgtt tgtcaacgtc agagtggagt
    14581 gatgaagaaa tacaaaagat atatcctagc cgcgtagaat tagtaagtaa cgctatgaat
    14641 ggtgcatcaa gtgatatcgt caagcagaag attacacatg tttttgatcc gtttttcgat
    14701 atgaacattg gcgctgcatt atattactta caaactggtg ggacatatgt tacttgtggt
    14761 ttacgagatc aacatccact attgtccaat gacacaccaa gtgatgcgga acctatggtc
    14821 agaggagcat tacaaatgag tatttttaag aatgtttcta ttttgggaaa ttgcctaggg
    14881 tcaaaagaag atttagaaaa agcaattcag ctacaaaata aaataatcat cgatcaggaa
    14941 tatcaactag aaaaaggtgt agattttgta caacgctctt tttttgattc ctcccgattt
    15001 ggaaaatgtg ttctgtttct ctaaaaataa tttagtatag gaagggtttg tcgtgtcaac
    15061 aataaaatgg attttactat tttttctatc tacaggaatt ggtacatttg tataccggat
    15121 aatggagaat ataaatataa acatttgggt atctagagcc actggttgtc tcgtttcatt
    15181 aatcgttgca cttcttctat acaaatattt caataggaga taatttttca atggagtagt
    15241 aactttttaa atgatgattt tcaataaggg gttataagcc gttcgtaaat gtgtgtactt
    15301 ttacagacgt tttaatgttt ttccattaga aatcacgatt attgaataac gtattgtaca
    15361 aatgactgta gttttaaatg atgtggatta agcaattggg cgcagaaatc ggtacgaaac
    15421 gttcctgact caaatgaaat gtggtcacac aatttcgctg aaggtcaggc agtttatggc
    15481 ccgattgctg tataacctta tataaaaccc cttcagattg aagtgacccc tgaaaacggg
    15541 acacatatca aaacacctcc attgaattcg catactaagt atgcaaattc aatggaggtg
    15601 tttttggttt gaaaacaaga gttcattacc cagaagaagt aaagtggaaa gtgattgaaa
    15661 tgaaaaagga tgggtattcc aatcggacca ttatggagaa gcttggaatt aaaaatgttt
    15721 cccaaattaa gacatggatg aaatagtatc gcaccggtca aacgtaccgt tttcaacaac
    15781 ctgtaggaaa acaatatgct tatggaaaag gaccaaacga acttagtgaa ttagaaaagc
    15841 tacgattaga aaataaacat ttaaaaacaa aattacttgt atggggaaag tatctggaaa
    15901 tcgaaaggag ctgaaaccac ataccgttat tactctatgg aatgaattaa aaacaaaggt
    15961 aaccgcccaa gaactgtgta aagtattaga attaccgcgt tctacatttt atcgttggtt
    16021 acagataact gaacgctcta aagatgaggt agaagaaaga ataaagaaag tctttattag
    16081 ctctttatgt ttagtgagta aacatggtag ttcttattcc ttacaataaa gtgtgtaagt
    16141 tggatacatc tatgctattt acatttcgcc ctccatatat ttaagtatcc ttttatatta
    16201 ctaataatat aaccaactaa atatggtaat acagttttag ttggttatat attttttgat
    16261 gtataacaaa aatggtttaa atgataacaa aattacttct gaaaaccgaa ctgcaatgta
    16321 ataaaaatat ttgttatagt tattgtagta aggaaaggta ggaagaggat atgtccctac
    16381 aacaagagtt acagattaaa gaaaataata ttgtttcgaa atccaatact ttaattgagg
    16441 caagttcacg tttaaattta gtagaacaaa agatgctgct ttgcttggca agtaatattg
    16501 aaccaagtga tcgggatttt aaaacatata catttccaat taaac
//
