LOCUS       NZ_NUIO01000011        10279 bp    DNA     linear   CON 20-MAY-2024
DEFINITION  Bacillus thuringiensis strain AFS049207
            AFS049207_100_C7_Contig14_140514, whole genome shotgun sequence.
ACCESSION   NZ_NUIO01000011
VERSION     NZ_NUIO01000011.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 71171)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 71171)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Assembly Cell v. 4.2.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64.45x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002575705.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 01:16:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,769
            CDSs (total)                      :: 5,694
            Genes (coding)                    :: 5,513
            CDSs (with protein)               :: 5,513
            Genes (RNA)                       :: 75
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 72 of 182
            Pseudo Genes (incomplete)         :: 113 of 182
            Pseudo Genes (internal stop)      :: 64 of 182
            Pseudo Genes (multiple problems)  :: 54 of 182
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:44:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 59087
            Orig. end                         :: 69366
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NUIO01000011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10279
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10279
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10279
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5279](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5279
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            977..2338
                     /locus_tag="CN977_RS13970"
                     /old_locus_tag="CN977_13975"
     CDS             977..2338
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017763222.1"
                     /locus_tag="CN977_RS13970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_13975"
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_074790178.1"
                     /transl_table=11
                     /translation="MNGKRNIFTCISIIGIGLASFSNSSFAANVTDNSVQNSIPVVNQQ
                     VAAAKEMKPFPQQVNYAGVIKPNHVTQESLNASVRSYYDNWKKKYLKNDLSSLPGGYYV
                     KGEITGDADGFKPLGTSEGQGYGMIITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNL
                     MGWVVADSKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGTVNYLKEAQDMITKGIKAS
                     NVTNNSRLNLGDWDSKNSLDTRPSDWMMSHLRAFYEFTGDKTWLTVINNLYDVYTQFSN
                     KYSPNTGLISDFVVKNPPQPAPKDFLDESEYTNAYYYNASRVPLRIVMDYAMYGEKRSK
                     VISDKVSSWIQNKTNGNPSKIVDGYQLNGSNIGSYSTAVFVSPFIAASITSSNNQKWVN
                     SGWDWMKNKRESYFSDSYNLLTMLFITGNWWKPVPDDKKIQNQINDAIYEGYDN"
     gene            2501..3424
                     /locus_tag="CN977_RS13975"
                     /old_locus_tag="CN977_13980"
     CDS             2501..3424
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="CN977_RS13975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_13980"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_086397133.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAV
                     HNAAYILQLANRQDIPVISGATKPLTEEITTYYPEIHGAEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGKDLTIVDVGRSTSLAIAFNLWNDLMLNVKAIYFMGGVFLEVGNVTPLA
                     EANVYGDPIASKIVFHQAKNLLIFPLNVTNKAVLTPNVFSYIQANTKNPFHMIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVVAISGLVNPSFFEFVHRTVNVDTYGDTKGQTFADFRPS
                     SIPEGGHIALEIDEQKFIQDFIKIML"
     gene            3502..3960
                     /locus_tag="CN977_RS13980"
                     /old_locus_tag="CN977_13985"
     CDS             3502..3960
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="CN977_RS13980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_13985"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_074790181.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRENWEEALKLQVKENQLKFVPSVAVSLTKVYIKPDG
                     DNVEYIPFAIYVGDLMVGFVMHAVVRKTSDMYWINGFIIDQSQQGNGYGKAALQESINI
                     IKNTFKSCKEIRLTVHKDNISAKKLYERYSFKSLEQEYDGEQVYRLFV"
     gene            3991..4338
                     /gene="cutA"
                     /locus_tag="CN977_RS13985"
                     /old_locus_tag="CN977_13990"
     CDS             3991..4338
                     /GO_process="GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="cutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160776.1"
                     /locus_tag="CN977_RS13985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_13990"
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="WP_001160789.1"
                     /transl_table=11
                     /translation="MQFTEVKIEVFIPEEYIETLRDELNKIGACKTGEYDHCLSYSSVK
                     GYWRPLDEASPFNGEIGRICEGQECKVEIKCKRELVKDALEVINDIHPYETPMIYIIPI
                     LNDYFEQLYIE"
     gene            4355..4801
                     /locus_tag="CN977_RS13990"
                     /old_locus_tag="CN977_13995"
     CDS             4355..4801
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="CN977_RS13990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_13995"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_000836826.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAVMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LIRELREELGVNCSIQQFLGVIENQWQDKEVLHHEINHIFEVESQDLHTDLPPKSSESH
                     LAFQWIDCNKEALNHYEIMPMPLVKELLERKLSDELLNCWISNF"
     gene            5001..5279
                     /locus_tag="CN977_RS13995"
                     /old_locus_tag="CN977_14000"
     CDS             5001..5279
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071381.1"
                     /locus_tag="CN977_RS13995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_14000"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_142338638.1"
                     /sec_met_domain="TIGR03601 (E-value: 2.8e-38, bitscore:
                     122.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQHELQTLNLNDYQTGNVVYWDQQQSQYPYYYIQDDARRCGG
                     CGGCGGRCGGCGGGRCGGCGGCGGCGGRCVGCAGCFGCFNCWNWWII"
     gene            5381..6397
                     /locus_tag="CN977_RS14000"
                     /old_locus_tag="CN977_14005"
     CDS             5381..6397
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="CN977_RS14000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_14005"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_016077591.1"
                     /transl_table=11
                     /translation="MYTYEKLISWVENIKEKNQSSAAALCIIKDNKIVLEHYSGYHSNT
                     STSKKVTAPSQFNVASARKSYLGLIIAYALYEGKINSIDDEAIKYFKDFDPVLLGKTTI
                     RHLVTHSHGLEETNDGTIFREFEPGQSWAYRDINVRMMTLLIYQLYNKSFPELLKERVF
                     KPAKFQETGWRIQQDENLVEIINNPNEDPISGIGTVNDGTEKNLFVSAREFAQWGNLHL
                     NQGMIHDKQIIPKEVIKIATSLQSPTFINKELPQNGVFWFIQNEPAQLSELGERIPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSNRIRA"
     gene            6429..6572
                     /locus_tag="CN977_RS30275"
                     /old_locus_tag="CN977_14010"
     CDS             6429..6572
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="CN977_RS30275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_14010"
                     /product="hypothetical protein"
                     /protein_id="WP_016077590.1"
                     /transl_table=11
                     /translation="MKDKVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFGEI
                     EY"
     gene            6853..8061
                     /locus_tag="CN977_RS14010"
                     /old_locus_tag="CN977_14015"
     CDS             6853..8061
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="CN977_RS14010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_14015"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_000814378.1"
                     /transl_table=11
                     /translation="MKQSSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAAKLQKKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLIDNLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNAVTETLHNDYTPREMMRTKQVYLLFFMLFTS
                     CMGGLYLISMVKDIGVQLVGLSAATAANAVAMIAIFNTVGRIILGTLSDKIGRMKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGAILGGFQPTFIIIGVLSVISFIISILIRPPTAEKKKELKHLHR
                     KVA"
     gene            8192..8569
                     /locus_tag="CN977_RS14015"
                     /old_locus_tag="CN977_14020"
     CDS             8192..8569
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706722.1"
                     /locus_tag="CN977_RS14015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_14020"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_000706908.1"
                     /transl_table=11
                     /translation="MKIVQQWVQEDSDYIREKVIEYNQKYLADEEKTPSEKVSFIVRNE
                     KEEIVGGVTEEYRHEGYGNKLIKLIEEFAIEKGCRLINLDTFSFQAPDFYKKHGYKVIG
                     VSEDHPKGHNHYYLEKRLKSI"
     misc_feature    complement(8752..8770)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.4"
                     /tool="antismash"
     gene            8851..9285
                     /locus_tag="CN977_RS14020"
                     /old_locus_tag="CN977_14025"
     CDS             8851..9285
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="CN977_RS14020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN977_14025"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_098829345.1"
                     /transl_table=11
                     /translation="MKKVNVTYAILYDKTNEKILMVKNKGENGSYYPLPGGAVKLGETL
                     EEAVIREVKEETGLHINVKGICSISEAFFEERDHHAIFFNFLGEIIGGETYISRPKEIE
                     EITWMELHIAAPYLRIPEHLLDLLQKKETVPYFFNGTIVH"
     misc_feature    complement(9477..9490)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.32"
                     /tool="antismash"
     misc_feature    complement(10233..10247)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 22.53"
                     /tool="antismash"
ORIGIN
        1 ggcaattcgt atggggcttg aatcttatac acctgagcat cttactttac tacgcttatt
       61 aatcgcttca tttatacttc tgttgttttc ttttatctac aagttacggc taccggattt
      121 aaaggatatc ccagcaattt ttatgttcgg tgccttagga ttcacttttt atcacattgc
      181 attaaactac ggcgaaaaaa cagtaaatgc tggttctgca agtttaattg tttcggtcac
      241 acctatagta acggcaattc ttgcttctat atttttaaac gaaaaaatga aattaaatgg
      301 ttggatcggt ggtgtaatta gttttatagg aattgctctc atatcattta gtcaaggaga
      361 tagtattcaa ttaaatagtg ggggattatt tatattattt gcagcaattt cagaaagcct
      421 atattttgtt ttccaaactt cttacttaaa aaagtacggc ttcttaccat ttaccatata
      481 tacaatttta tctagtactg tatgtatgct tattttctta ccgggaatgt atacagaaat
      541 actagcagct cctcttgaag tgaatgtgag tgttttatat ttaggcttat ttccaacagt
      601 actcccatat atcgcattgg cctatatcat atctcatgct ggtgctgctg aggcaacaag
      661 ttctctatat ttaacgccag taactgcatg ttttattgct tggttatggt taggagaagt
      721 gccaacttta gtttcgataa ttggcggagg gattactata cttggtatcg tgattgctca
      781 tataccagta ttaagaaaag aaaaacatag gaatttagca aataatcaat ccgtataacg
      841 taattcaata ataattttat gatacttttt catatagttt ctttcttatg ttgataaata
      901 taatattttg tatttcagtt aaatattgta tgttacattt tatgtagtaa gcattttaaa
      961 aggagatgac aacataatga atggaaaaag aaatattttc acatgtattt ctattatagg
     1021 aatcggtcta gctagttttt ctaattctag tttcgcagca aatgtaacgg acaattcagt
     1081 acaaaattct attcccgtag ttaatcaaca agtagctgct gcaaaggaaa tgaaaccatt
     1141 tccccagcaa gttaattatg caggtgttat aaaaccgaat catgttacac aggaaagttt
     1201 aaatgcttct gtaagaagtt actacgataa ttggaaaaag aaatatttga aaaatgattt
     1261 atcttcttta cctggtggtt attatgtaaa aggagagatt acaggtgatg cggatgggtt
     1321 taagccactt ggaacttcag aaggtcaagg gtatgggatg ataattacag tattaatggc
     1381 tggttatgat tcgaatgctc aaaaaatcta tgacggttta tttaaaacag caagaacttt
     1441 taaaagttct caaaatccta atttaatggg atgggttgtt gcagatagta aaaaagcaca
     1501 aggtcatttt gattctgcta ctgatgggga tttagatatt gcgtattctc ttcttcttgc
     1561 tcataagcag tggggatcaa atggaacagt taattattta aaagaagcac aagacatgat
     1621 tacaaaaggt attaaagcta gtaatgttac aaataatagc cgactaaatt taggagattg
     1681 ggattctaaa aattcacttg atacgaggcc atctgattgg atgatgtcac accttagagc
     1741 attttatgaa tttacaggtg ataaaacttg gcttactgtt attaataatt tgtacgatgt
     1801 ttatacgcaa tttagtaata agtactctcc aaatacagga cttatttcag atttcgttgt
     1861 aaaaaaccca ccacaacccg cacctaaaga cttcttagat gagtcagaat atacaaatgc
     1921 atattattac aatgctagtc gggtaccatt gagaattgta atggactatg cgatgtacgg
     1981 cgagaaaaga agtaaagtca tttctgataa agtctcttca tggattcaaa ataaaacgaa
     2041 tggaaatcct tctaaaattg tggatggtta tcaattaaat ggatctaata ttggtagtta
     2101 ttcaactgct gtatttgttt caccgtttat tgctgcaagt ataacgagta gcaataatca
     2161 aaagtgggta aatagcggtt gggattggat gaagaataag agagaaagct attttagtga
     2221 tagttataat ttattaacta tgttatttat tacaggaaat tggtggaaac ctgtacctga
     2281 tgataaaaaa atacaaaatc aaataaatga tgcaatttat gaaggatacg ataattaatt
     2341 aaatttgaga atggttactt aagaaaggaa ttagcatacg ctagttcctt tttaatattt
     2401 tgtaaaacat tatccattct aaaattcgca tagatatatt tgcttttttc atatacatag
     2461 ttattgatga ctctatgttt ttatgagggg agagtatttc ttggaaaaag tattattttt
     2521 cggagaccca ggtattgatg actcatttgc cattatgtat ggtttactgc atcccgaaat
     2581 tgaaattgta ggtattgtaa ctggatacgg aaatgttgag catatacatg ctgtacataa
     2641 tgcagcgtat attttacagt tagcaaatag acaagacatt cctgtcatta gtggtgcaac
     2701 aaaaccactt acagaagaaa ttactacgta ctaccctgaa attcacggag cagaaggatt
     2761 agggcccatc catgttccag aaagcgtatt atctattcca atatacaatt ttggtagtat
     2821 cgctgcgatt cttgtaaagt acggaaaaga tttaacgatt gttgatgtag gaagatctac
     2881 ctcgttagca attgctttta atttatggaa tgatttaatg ctaaatgtaa aagcaatata
     2941 tttcatgggt ggtgtttttt tagaagtagg aaacgttacg ccgttagcag aggcaaatgt
     3001 gtatggagat cctatagcaa gtaaaattgt tttccatcaa gcaaagaatt tattaatatt
     3061 ccctttaaat gtaacaaaca aagctgtgtt aacaccaaat gtatttagct atattcaagc
     3121 taatacaaag aatccatttc atatgataat gaaaccaatg tatgattact atttatctgc
     3181 ataccaaaaa cttaatccat ctatcgaagg acctttgcta catgatgtgg ttgcaataag
     3241 tggattagta aatccaagtt tttttgaatt tgtgcatcgt acagtaaatg tagatacata
     3301 cggagatacg aaaggacaaa catttgctga tttccgccct agctctatac ctgaaggggg
     3361 ccatatagca ttagaaatag atgaacaaaa attcattcaa gattttataa aaatcatgtt
     3421 ataagttgtt gtcgaaaaag ggaattattt caatgcgttg aatataaaaa tacatccctc
     3481 tagataaagg agcttacaat attgaatgtc cagttaaagg ttgttacgag agaaaattgg
     3541 gaagaagcat taaagctaca agttaaagaa aatcagttga aatttgttcc atccgtagca
     3601 gtttcgctta ctaaagtata tataaaacca gatggtgaca atgtagagta cataccattc
     3661 gctatatacg ttggtgacct tatggttggt tttgtaatgc atgcagttgt aagaaagaca
     3721 tcagatatgt attggattaa cggatttatt attgatcaat cgcagcaagg taatggatat
     3781 ggaaaagcag cattacaaga aagtattaat ataataaaaa atacttttaa atcgtgtaaa
     3841 gaaattagat taaccgtaca taaagataat atctctgcaa agaaactata tgaacgatac
     3901 agtttcaaat cattagaaca ggagtatgat ggcgagcaag tatatcgttt atttgtttaa
     3961 ctattaggaa gtaaaggcat gaaatgaaat atgcaattta ctgaagtgaa aattgaagtg
     4021 ttcattcctg aagaatatat tgaaacatta agggatgaat taaataaaat tggagcatgt
     4081 aaaacgggcg aatatgacca ttgtctttcc tatagttcag tgaaaggata ttggagacct
     4141 ttagacgagg catctccttt taatggagaa attggtcgaa tatgcgaagg gcaagaatgt
     4201 aaagttgaaa taaagtgtaa acgagaactt gtaaaagatg ctcttgaagt tattaatgat
     4261 attcatccgt atgaaacacc tatgatttat attataccaa tactaaatga ctattttgag
     4321 caactatata ttgaataact aggaggaata tcaattgaaa agtaaatttc atcatattgt
     4381 acgagcagtc atgataaaag atgaaaaatt attagttgct gaatatattg gccatcatta
     4441 ttttttacct ggtggccatg ttgaaattgg agaatcagca gagaatgcat taataagaga
     4501 gttaagagaa gaacttggag taaactgtag tatacaacaa tttttaggag tcatagaaaa
     4561 tcaatggcaa gataaagaag tgcttcatca tgaaattaac cacatttttg aagtagagtc
     4621 acaagattta catacggact taccaccgaa atctagcgag tctcatttag catttcaatg
     4681 gatagattgt aataaagaag ccttaaatca ttatgaaatc atgccaatgc ctttagtgaa
     4741 ggaattacta gaaagaaagt taagtgatga actactaaat tgttggatta gtaattttta
     4801 aatcacttta tttatagtaa atacgtccat cgaatgaata taaagaaaat aggctgaaaa
     4861 acaaggtaca tcacctagtg taagttcata ttttgttgat gtacgcattt agtctatagt
     4921 cttgtttcta ctttgttcac aagaatttat taactttaat atttgggcgg aatttatatt
     4981 tataaatgga ggggttctta atgaatcagt ttcaacatga actacaaaca ttaaatctta
     5041 atgattatca aactggtaat gttgtgtatt gggatcaaca acaaagtcaa tatccatact
     5101 actacattca agacgatgca cgtcgttgcg gaggatgcgg aggttgtggt ggacgctgtg
     5161 gcggttgtgg cggcggtaga tgtggtggct gtggtggctg tggtggctgt ggtggacgtt
     5221 gcgtaggttg cgctggttgt tttggttgct ttaattgctg gaactggtgg attatttaaa
     5281 tatagtacat cattaatatt ttatatgaaa gaaaggtgta aatactgttt atacttttct
     5341 ttcatgctgt aattttgtat tagtgttaag ggggatttat ttgtatacat acgaaaagtt
     5401 gatttcttgg gtagaaaata taaaagaaaa aaatcaaagt tctgctgcag cactttgtat
     5461 tataaaagat aataaaatcg tgctagagca ttatagtggt tatcactcaa atacatctac
     5521 aagcaagaaa gtaacagcac cttcacaatt taacgttgct tctgctagaa aaagttattt
     5581 aggattaata atagcgtatg cgctttatga ggggaaaata aactctattg atgatgaagc
     5641 gataaaatat tttaaagact ttgatcctgt attgcttggt aaaacgacga taagacattt
     5701 agtaacacat tcgcacggat tagaagaaac gaatgacggg acaatttttc gtgaatttga
     5761 accgggacaa tcatgggcgt atagagatat taatgtaaga atgatgacac ttcttattta
     5821 tcagctatat aacaaaagtt ttcctgaatt gttaaaagag cgtgttttta aacctgctaa
     5881 atttcaagaa acaggatgga gaatacagca agatgaaaat ttggttgaaa ttattaataa
     5941 tccaaatgaa gacccgataa gtggaattgg tacagtaaat gatggtactg aaaaaaatct
     6001 atttgtctca gctagagaat ttgcgcagtg gggcaatctt catctaaatc aaggcatgat
     6061 acatgataaa caaatcattc caaaagaagt tataaaaatt gctacgagtt tgcagagtcc
     6121 aacatttata aacaaagagc taccacaaaa tggggtgttt tggttcatcc aaaatgagcc
     6181 tgcacaatta agcgaacttg gtgaacgtat tccaaaaggg tcgtatcaaa tattagggat
     6241 tactggaccg actattttag taatacctga atataatgta gttgttgcaa aaatgtataa
     6301 taaaagatat aactacggcg gtgataatta cttatattat ttacgtgaat ttagtaattt
     6361 agtcgctgat acatttagta accgtattag ggcataatta ctcctataac aaaaaggagt
     6421 gattgaaagt gaaagataaa gtgaacaaaa ataaaaagca aaatataaaa gatgtaaata
     6481 cagagcaaaa tgcaatttat agcgatccta aagatgcagc taatatgcaa actgtaccgc
     6541 agccgaaaga ttttggtgaa atcgaatatt aatttttcat ccgactatat gatgaatgaa
     6601 aaatacgtct taagtctcat aacatttaag aaattatttg ataacatata tacataagca
     6661 tttgaaaatg tcttatctta acaaagcttt ctttagaaat atctgatata acctgcaaaa
     6721 ggcacttctt tatgaagtgt ctttttgtgt atatgaactt aattaccatt ttccgtacag
     6781 tatcttgtaa tttatgatgg atatactaac tgtgaaaatt catgtcaaga taaaagaagg
     6841 ggacaagtta gtatgaaaca aagttctata aatccgttac taattgttct aggtacaatc
     6901 attgttcaaa ttggccttgg aacaatttat acatggagtt tatttaatca accccttgta
     6961 agtaagtttg gatggaatct taattcagta gcgataactt tctccataac aagtttttct
     7021 ttatcattct caactttatt tgcagcaaag ttgcagaaaa aattaggact tcgaaaactt
     7081 attgctactg caggaattgt tttaggactc ggcttaatac ttagttcaca agtttcttct
     7141 ttaccactat tatatttatt agctggtgtc gttgttggtt atgcggatgg aacagcttat
     7201 attacatcac tatctaattt aattaaatgg tttccaaatc ggaaagggct tatttcaggt
     7261 atatctgtat cagcgtatgg aatgggcagc ttaatcttta gatatataaa cggaaatctt
     7321 atcgataacc ttggtgtatc acaagcattc ttatattggg gtattatcgt gttactttta
     7381 gtgttaatcg gatcgttctt cttacgtgag gcaattgtaa gtaatgctgt aactgaaaca
     7441 ttacacaatg actatactcc gcgtgaaatg atgcgaacga aacaagtata tctcctattt
     7501 tttatgttat tcacctcgtg tatgggtggt ttgtatttaa taagtatggt aaaagatatc
     7561 ggcgtacaac tcgttggact tagcgcagca accgctgcaa acgccgttgc tatgattgca
     7621 atctttaata cagtaggtag aattattctt gggacgctat cagataaaat cggccgaatg
     7681 aaaattgtct ctgcaacgtt tattattata ggattgtcag tctttacttt aagttttatt
     7741 ccgctaaatt acggaatcta ttttgcttgt gtagcaagtg tcgccttttg cttcggtggt
     7801 aatataacta tattcccagc tattgtcgga gatttctttg gattaaaaaa ccatagtaca
     7861 aattacggca ttgtctacca aggtttcgga tttggtgcgc ttgcaggatc atttattgga
     7921 gcgatactcg ggggatttca accaactttc attataatcg gtgttttaag tgttatttcc
     7981 ttcattattt caatattaat ccgtccacca actgcagaaa agaaaaagga actaaaacat
     8041 ttacatcgga aagttgctta attttaaaaa gagtcctcat gttagggctc ttttttattc
     8101 cattaacttt gcttctttac taactcatta aagacaggac aatatcatta taaaattgaa
     8161 tagataagta aaaaggagcg tgacacttaa aatgaaaata gtacagcagt gggtacaaga
     8221 agatagtgat tacataagag agaaagtaat tgaatataat caaaaatatc tcgcagatga
     8281 agaaaaaaca ccttcagaaa aggttagttt catagtaaga aatgaaaaag aagaaatcgt
     8341 aggtggggta actgaagaat ataggcatga aggttacgga aacaagttaa taaagcttat
     8401 tgaagaattt gcaattgaaa aagggtgtag gttaattaat ttagatacct ttagtttcca
     8461 agctcctgat ttttataaga aacatggata taaagtaatt ggagtaagtg aagatcatcc
     8521 gaaaggacat aatcattatt atttagaaaa acggctaaaa agtatataga atttgttaac
     8581 attagtattt ggaatacaaa tgtaatcaag gcgaggttat tattaatttt agttttaatt
     8641 taatcaattt tcatgctaaa acttcaagaa cgttctttgg ataatcttcc taagatacgt
     8701 tctttcttta tatatcagtg gcccattctt attaactcca ccaatagcgg taaaggcaag
     8761 acttaactaa cttatatata ttaaaaggag ttaatggtag aatggataga agacagaaaa
     8821 ttccttttat taaaaataga ggaggtacat atgaaaaaag ttaatgttac atacgcgatt
     8881 ttgtacgata aaacaaacga aaagatctta atggtaaaaa acaaaggaga aaatggttct
     8941 tattatccat taccaggtgg cgcagttaaa ttaggagaaa cattggaaga agcagtaatt
     9001 cgagaagtaa aagaagaaac agggttacat ataaacgtaa aagggatttg ttctatcagc
     9061 gaagcctttt tcgaggaaag agatcatcat gcaattttct ttaattttct aggcgaaata
     9121 atcggcgggg agacatatat atcaagacca aaagaaatcg aagagattac ttggatggaa
     9181 ttacatatag cagcaccgta tttacgcata ccagaacatt tactagattt attacaaaag
     9241 aaagaaacag taccttattt ttttaacgga acaatcgttc attaatcttc ataaaaatat
     9301 tatctcatgt aaggtgggga aaacatggag ttcatcgtta atcacaaaca atttactcaa
     9361 gcactttcag aagttaacaa agcgatatca acaaaatctt taattcctat attatctggt
     9421 ataaaaataa ctgcagatca atctggaatt actttaattg caagtaactc gaatattttt
     9481 attgaaaaat tcattcctgt ttcaattgaa gacgaaaaaa ttgcaactat tttaaaggca
     9541 ggaactattg ttgtacctgc aaagtatttc attgaaataa taaagaaaat gccaagtgat
     9601 atagcaataa aaagtaagaa tgagcaaatt attaagatac aatcagaaga aatcacatta
     9661 agtttaaatg gatttcctgc agatgagttt ccgaatgtac cgcttataga caatcatgca
     9721 gaaatcaagg tagagacaga gcaattgatt gaagtattta aacaaacagt ctttgcggta
     9781 gcgaaaaatg aatctagacc tgttcttact ggggtgcata tagagttatc taataataag
     9841 tttatttgtg ctgcaactga ctcacataga ctagcgatac gtgaaacgcg actttcctca
     9901 gatgtaaaag caaattgtat cgtaccaagc gcaaccatta gtgaacttct aaaattaatg
     9961 aacagcaatt cggaattcgt atatatttac ctttcagaga gtcatattat atttacgctt
    10021 ggaacaacta cattatattc aagactaatt gaagggaaat atcctaatat ttctaacctt
    10081 attccaaatg actttaaaac gattattaac atagatagaa aaaagatatt acaaggtgta
    10141 gatcgatcaa gtttattagc aagtgaatgg gccaataaca atgttaactt agaaatcata
    10201 aacgaatcta caattaaaat ttcttcaaac gcttctcaaa ttgggcaaat atccgaaacg
    10261 caacaaatag atgcgattc
//
