LOCUS       NZ_MTJS01000002        20858 bp    DNA     linear   CON 23-AUG-2024
DEFINITION  Lactococcus lactis strain 537 contig_8, whole genome shotgun
            sequence.
ACCESSION   NZ_MTJS01000002
VERSION     NZ_MTJS01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Lactococcus lactis
  ORGANISM  Lactococcus lactis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Lactococcus.
REFERENCE   1  (bases 1 to 1672417)
  AUTHORS   Ho,V., Lo,R., Bansal,N. and Turner,M.S.
  TITLE     Characterization of Lactococcus lactis isolates from herbs, fruits
            and vegetables for use as biopreservatives against Listeria
            monocytogenes in cheese
  JOURNAL   Food Control (2017) In press
REFERENCE   2  (bases 1 to 1672417)
  AUTHORS   Lo,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2017) School of Agriculture and Food Sciences, The
            University of Queensland, Hartley Teakle Bldg (No. 83), Slip Rd, St
            Lucia, Brisbane, QLD 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. Jun 2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1036.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002563475.1-RS_2024_08_23
            Annotation Date                   :: 08/23/2024 15:27:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,579
            CDSs (total)                      :: 2,519
            Genes (coding)                    :: 2,482
            CDSs (with protein)               :: 2,482
            Genes (RNA)                       :: 60
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 51
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 14 of 37
            Pseudo Genes (incomplete)         :: 23 of 37
            Pseudo Genes (internal stop)      :: 8 of 37
            Pseudo Genes (multiple problems)  :: 7 of 37
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:09:48
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1237048
            Orig. end                         :: 1257906
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MTJS01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20858
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="2"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20858
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20858
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10858](-)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10858)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    216..231
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 19.86"
                     /tool="antismash"
     misc_feature    complement(302..319)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 23.05"
                     /tool="antismash"
     misc_feature    complement(524..540)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 21.16"
                     /tool="antismash"
     gene            complement(2219..2599)
                     /locus_tag="BW154_RS08610"
                     /old_locus_tag="BW154_08625"
     CDS             complement(2219..2599)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003131357.1"
                     /locus_tag="BW154_RS08610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08625"
                     /product="cell division protein FtsL"
                     /protein_id="WP_003131357.1"
                     /transl_table=11
                     /translation="MAAQPKEYFDIETAAKKDSYLIDTETLAPEVLASKFKKFSGVEKV
                     FYLTMVVAALVLAVGLLYVKTKTLEVQGNTMDLRSKISQETTRKTEYDQQILDLTSGTK
                     VLDTANKADLKQNPSNVLKATK"
     gene            complement(2757..3710)
                     /gene="rsmH"
                     /locus_tag="BW154_RS08615"
                     /old_locus_tag="BW154_08630"
     CDS             complement(2757..3710)
                     /EC_number="2.1.1.199"
                     /GO_function="GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="rsmH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017864364.1"
                     /locus_tag="BW154_RS08615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08630"
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="WP_080739867.1"
                     /transl_table=11
                     /translation="MPKTIAFKHDTVLLHETVDMLEVKPNGIYVDATLGGAGHSEYLLS
                     KLTDGHLYSFDQDETAHENAKVRLSEQLAEDKVTLIKSNFRYLKSALAELGVTKIDGIL
                     YDLGVSSPQFDDSQRGFSYKKEARLDMRMDQNQTLSAYEVVNDYPYEALVRIFFRYGED
                     KFSKQIARKIEQARKIKPIETTIELADLIKSALPQKELKKKGHPAKRIFQAIRIEVNDE
                     LGAAEESIEQAIDLLKVDGRISVITFHSLEDRLTKTIFKEYSTVNVPKGLPMLPKDMEA
                     KLKLVNRKPVLASEEELEFNNRAHSAKLRVAQKQRD"
     misc_feature    3794..3807
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.27"
                     /tool="antismash"
     gene            complement(3930..4868)
                     /locus_tag="BW154_RS08620"
                     /old_locus_tag="BW154_08635"
     CDS             complement(3930..4868)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017864363.1"
                     /locus_tag="BW154_RS08620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08635"
                     /product="DUF805 domain-containing protein"
                     /protein_id="WP_098393957.1"
                     /transl_table=11
                     /translation="MVQAYKKFWLGAFTFNRKTSRKDFWSALLTHIIIFVILFKAYHFF
                     NLLDFYQLTTLWQTFASFFQLVFNLYFFGSLLSFIALTVRRLNDADLPWALIFLNFILG
                     LGTLVLLVLNLLPSSPRALKFEEYKINSSQEFNNLPETKTLSGIFKDYFKNYFEFRGRT
                     TRRNFWWMQLFWGLTFILFLFLIYLFNQFEQIMFGYNFIGSMVLRLFFFLFILGTFFPQ
                     LTIHVRRLRDDGLSNLGLSLLLGGTSGILIFYQMFTKALKITYITGHYQLVQYLLFLLV
                     MIAVLSLILVEVMATGELKTNKKNSLFEKID"
     gene            complement(4892..5413)
                     /locus_tag="BW154_RS08625"
                     /old_locus_tag="BW154_08640"
     CDS             complement(4892..5413)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015426163.1"
                     /locus_tag="BW154_RS08625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08640"
                     /product="DUF805 domain-containing protein"
                     /protein_id="WP_058205392.1"
                     /transl_table=11
                     /translation="MIKAYIKYWKKAGDLKSYSNRSDYWWVFLTNSIIFAILSILNFMV
                     TIPKAGKIMSQSATLSQKEIIQQVTDLYANPTGGALVIVIVTALIGLAILVPNISLTAR
                     RLRDARFPWWIALIFGVAAIYGLVTMFIHQDFLAKMGYFVGFINFIIYILCLFPTKYGI
                     DEEDDSRTYE"
     gene            complement(5511..6077)
                     /locus_tag="BW154_RS08630"
                     /old_locus_tag="BW154_08645"
     CDS             complement(5511..6077)
                     /GO_process="GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011675826.1"
                     /locus_tag="BW154_RS08630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08645"
                     /product="TMEM175 family protein"
                     /protein_id="WP_038601472.1"
                     /transl_table=11
                     /translation="MSKTRLEAFTDAVIAIIMTVLVLELVRPDSDSLQSFIALLPHLGV
                     YFVSFFILAIYWVNHHHLFQSIKRINGKVLWINISFLFVLSLIPIFSNWVSIYPNSFIP
                     ELGYVIIFFLGNFIYLLLTRELLKINGHRKTSETTIRKNILSVVLNIVSILLGYFIAPV
                     IMLVGSALIFSMWVIPDKNIEKMFK"
     gene            complement(6419..>6601)
                     /locus_tag="BW154_RS13105"
                     /old_locus_tag="BW154_08650"
                     /pseudo=""
     CDS             complement(6419..>6601)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015426162.1"
                     /locus_tag="BW154_RS13105"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08650"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="PVFFFFFIYEHYNQNYFFLFVGIVGACAGIICMLCEMLIQILRNQ
                     KIKNKLKKLSIDKFL"
     assembly_gap    6602..6736
                     /estimated_length=135
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6871..8538)
                     /gene="recN"
                     /locus_tag="BW154_RS08640"
                     /old_locus_tag="BW154_08655"
     CDS             complement(6871..8538)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019292004.1"
                     /locus_tag="BW154_RS08640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08655"
                     /product="DNA repair protein RecN"
                     /protein_id="WP_098393958.1"
                     /transl_table=11
                     /translation="MLQEISIKNFAIIEEIHLSFESGMTILTGETGAGKSIIIDAMSLL
                     LGGRASSDFVRHGANKAEIEGLFFFDKTPELKAVLLELGFEEVDSEIILRREIFANGRS
                     VCRINGQMVNLATLRQVGELLVDIHGQHDSQELMNPKSHLRLLDEFGDEAFDAIKDNYK
                     LKFENFKNLRQQLNLRQKNEREFAQRIEILQFQAEEIEAAEINLEEDEQLVNRREKLNN
                     IKNIADSLSSAYLALDDEDNDYSSLNNIRTTMTELDKISNFDNDYQELADKTAESYYVL
                     EEVANQIQRIMSDLEFNPAELLQIEDRIMTLTTLKKKYGPELSDVMNYLEKVQLELSEL
                     TGSEKDSENLENSVKVAQVQLIEASKKLNEARHKIAKEVEKDIKHELSELYMEKADFKV
                     SFEGAKFSANGNEHVEFFIQTNPGEGFKPLAKTASGGELSRLMLAIKSSFSRRENKTAI
                     VFDEVDTGVSGRVAQAIASKIYKIARHGQVLAISHLPQVVAVADRQFYIEKASTDDITT
                     STVRKLNFDERVNEIAKMLAGEDLTKEALAQAKRLLSK"
     gene            complement(8687..9133)
                     /locus_tag="BW154_RS08645"
                     /old_locus_tag="BW154_08660"
     CDS             complement(8687..9133)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015426160.1"
                     /locus_tag="BW154_RS08645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08660"
                     /product="arginine repressor"
                     /protein_id="WP_038601476.1"
                     /transl_table=11
                     /translation="MKREDRLNFIAQFIRENEIKTQEELVNTLLTHGIDVTQATVSRDI
                     KSLALIKVPAESGGYRYDLPKNKEVLQSSLHKALAFDAITGTKIKDNMLWILANPGTTS
                     LVKNYLLEEYGDDIFSIIIDDNSALVIFEMEEDAKNLYNLLTEF"
     gene            complement(9130..9936)
                     /locus_tag="BW154_RS08650"
                     /old_locus_tag="BW154_08665"
     CDS             complement(9130..9936)
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003131349.1"
                     /locus_tag="BW154_RS08650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08665"
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="WP_038601477.1"
                     /transl_table=11
                     /translation="MKERIDVLATNQGLFETREQAKRGVMAGLVVDSKSGERYDKPGQK
                     IDDGTELRLKGEKLRYVSRGGLKLEKALKEFHLEINGKTCLDIGSSTGGFTDVMLQNGA
                     KLVYALDVGTNQLAWKIRSDERVVVMEQFNFRNAVLADFGQGRPSFTSIDVSFISLDLI
                     LPPLYEILEENGEVAALIKPQFEAGREQVGKNGIIRDSKVHQMTIEKVLKTATQLGFSV
                     KGLTFSPIKGGAGNVEFLVHLLKDGKAEIAQQVNIESVLQKESEEL"
     misc_feature    complement(9949..9962)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 24.74"
                     /tool="antismash"
     gene            complement(10001..10858)
                     /locus_tag="BW154_RS08655"
                     /old_locus_tag="BW154_08670"
     CDS             complement(10001..10858)
                     /EC_number="2.5.1.-"
                     /GO_function="GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012897509.1"
                     /locus_tag="BW154_RS08655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08670"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_098393959.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.9e-53, bitscore:
                     178.0, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTKIRKLEDFLTEFYESAEFPTGLAESAKYSLLAGGKRIRPLLF
                     LNLLEAFNLELSKAHYHVAAALEMIHTGSLIHDDLPAMDNDDYRRGQLTNHKKFDEATA
                     ILAGDTLFFDPFFILSTADLSAEIIVALTRELAFASGSYGMVAGQILDMAGEGKELTLA
                     EIEQIHRLKTGRLLTFPFVAAGIVAQKSTDEVEKLRQVGQILGLAFQIRDDILDVTATF
                     AELGKTPGKDILEEKSTYVAHLGLEGAKKSLTENLSEVKKLLTALSVTDSSEIFKIIEQ
                     LAVK"
     gene            complement(10939..12096)
                     /locus_tag="BW154_RS08660"
                     /old_locus_tag="BW154_08675"
     CDS             complement(10939..12096)
                     /EC_number="2.7.1.31"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017864361.1"
                     /locus_tag="BW154_RS08660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08675"
                     /product="glycerate kinase"
                     /protein_id="WP_038601481.1"
                     /transl_table=11
                     /translation="MKKKFVLAPDSFKESLSALEVCQAMEKGIKKVFPEAEIISMPMAD
                     GGEGTTDALIDATGGRRVYKKVQGSFANTNVEAYFGILGNSNTAVVEMAKANGLELVAL
                     EDRNPLITSTYGTGQLIKAAMDEGVDKIIIGIGGSATNDGGSGMARALGARFLNKDQEE
                     IPLGGAFLKEVRTIDMSDFDPRVEEIEILIATDVTNPLTGNNGASYVFGPQKGATEEMV
                     KVLDENLKHYAEIVHNNLGKDVKNIPGAGAAGGLGAGLLAFTNAKMQLGIELVAQTIYL
                     EDKIKEADYVFTGEGGMDYQTKFGKAPYGVCKIAKKYKKPCFALAGYIGDGAEVLYEEG
                     MTAIFGILSQAQSLSDALADGAKNVERTSQNITRVLAMNEQKAHL"
     gene            complement(12245..12484)
                     /locus_tag="BW154_RS08665"
                     /old_locus_tag="BW154_08680"
     CDS             complement(12245..12484)
                     /EC_number="3.1.11.6"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003131346.1"
                     /locus_tag="BW154_RS08665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08680"
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="WP_003131346.1"
                     /transl_table=11
                     /translation="MATKKEDVKFEDNLAELENIVRKLESGDVALEDAIAEFQKGMKIS
                     ETLKKTLNEAEETLVKIVGKNDQESDFSPEQKDY"
     gene            complement(12484..13737)
                     /gene="xseA"
                     /locus_tag="BW154_RS08670"
                     /old_locus_tag="BW154_08685"
     CDS             complement(12484..13737)
                     /EC_number="3.1.11.6"
                     /GO_component="GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA]"
                     /GO_function="GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /GO_process="GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="xseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003131345.1"
                     /locus_tag="BW154_RS08670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08685"
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="WP_098393960.1"
                     /transl_table=11
                     /translation="MTEYLSVSTLTKYLKAKFDRDPYLERVYLTGEISNFRRRPNHQYF
                     ALKDEGAVIQATMWAGQFRKLDFELEEGMKVLAVGRISIYPPSGSYSINIESLVPDGVG
                     ALAVKFEQLKKKLTAEGLFEQRWKQTLPQFSKKIAVVTSPSGAVIRDIITTVQRRFPMS
                     QIVLYPTKVQGQGSAEEIAENIRRANERGDFDVMIIGRGGGSIEDLWGFNEEIVVRAIF
                     ESRIPIISSVGHETDVTLADFVADSRAATPTAAAELATPNTKVDLINWANEQEKRLFNR
                     LTHLIKIRRERVDKLSQSVVFRQPERLYDGHLQKLDRLCERLSVLTENKVANMKHRYEL
                     SAGKLIPTYGKIVEAKKNKTEQLYQSLLLLDISKIKARGFSLVTDEKGKIIKSVSDVKK
                     GQTLDVELTDGKVIVEVK"
     gene            complement(13904..14758)
                     /locus_tag="BW154_RS08675"
                     /old_locus_tag="BW154_08690"
     CDS             complement(13904..14758)
                     /GO_function="GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003131344.1"
                     /locus_tag="BW154_RS08675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08690"
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="WP_038602888.1"
                     /transl_table=11
                     /translation="MNLIDGKALAAKMQAELKVKVDKLKEADNVPGLAVILVGEDPASQ
                     IYVRNKARQATAIGLNSSVVRLPETVTEQELLDLIEQYNQSEQWHGILVQLPLPEHISE
                     EKVLLAIDPEKDVDGFHPMNMGRLWSGNPLMIPSTPAGIMEMFREYNVELSGKRAVVIG
                     RSNIVGKPMAQLLMMADATVTIAHSKTENLRELTKEADILVVAIGRDRMIKAEDIKEGA
                     VVIDVGMNRDEDGKLHGDVDFDEVKNVASFITPVPGGVGPMTITMLMEQTVRAASRKMN
                     ENK"
     CDS             complement(14914..>16591)
                     /codon_start=3
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BW154_RS08680"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BW154_08695"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="GAIENDLSNTISQIQNSDILNQVVNQLQSAINNIVESATGIVNNL
                     PSLGAIENDLSNTISQIQNSDILNQVVNQLQSAINNIVESATGIVNNLPSLGAIENDLS
                     NIISQIQNNVNNFVNNALNGITTIINSLTPSVGASTVNPNSSANSSQSSSSASSSSSAA
                     SSSTSSSNVSSNTSSNSSEANTSSSTSNDSSSTSSESGVPSSSSVESSQTSSAGVNSSN
                     SSSESSSISSSTSSESSIASSSSVDSSQSSSAGVNGSSSSSEGSNTSSSNSSESSVASS
                     SSVDSSHSSSAGVSSSDSSSESSSISSSTSSESGIASSSSADSSHSSSVGISSSDSSSE
                     SSSSSSSTSSESSTASSSSVDSSHSSSAGVSSSDSSSEGSSVSSLSSSESSITNSSGIS
                     DSNSNTDQTSTTSPSSENSSHSSLSSNESSSASLTSSESSGSTTSSSMLTNPNLQANDK
                     NGSSLTSNSSYTSEVKLNNLSDSNLPSNEAKVSTLISKVSSSPSLSNSLSATSLQLVPT
                     VTKEELPKTGADDDLSLVLAELGLGLVATLTIGKVKYKKGFE"
     gene            complement(14914..>16595)
                     /locus_tag="BW154_RS08680"
                     /old_locus_tag="BW154_08695"
                     /pseudo=""
     assembly_gap    16596..16681
                     /estimated_length=86
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16682..18448)
                     /locus_tag="BW154_RS08685"
                     /old_locus_tag="BW154_08700"
     CDS             complement(<16682..18448)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015426153.1"
                     /locus_tag="BW154_RS08685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08700"
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
                     /protein_id="WP_179884627.1"
                     /transl_table=11
                     /translation="MKKIRPEVSTVISKSHFRTWKSGKSWLFATSALALIIVSGTITDQ
                     NVKADTAVDSSQQISGITEETSYSALASSTNSDVAASQNQVAYEQASDQSSNNSLANTV
                     ETDTEGMTSDVSDSSNSINESQNTISTAVIQTATNNIVSLADSSSSNDNISNSILSSSN
                     AADSVDSAAVSQSSTSSSVILSESSAIDSGIGSTSQSSEINLAENSSANGSSAAVASFT
                     AILATNSSVVPMLTQALAAAAPATTSGSAILNTTLGDLVNQAISTVGISGLENILNTLG
                     TFNIPGMTTAASALNGVEQIVNIVGNIQEAAANPGAFLLNGMKSAGLDVSQIPLVGGQI
                     VTAFNAIPSMSPAAMLNFLENPTIPGLSSIPGANLVLSPVLSAISTVTSGIVNQLNTTT
                     SNALGGVNFDIDTLVSLQGNDLVNYLAGLVVNSAINRAGQIAWSQLSPTISNIPLVGTT
                     VNNVLSPILNNLTGASLGEVANLTGVSSLLDQVNNSLGNLTSLGSTALATIENTLQNSL
                     NSLGNLPAGASDILNQVLNQLQNAINNIVESATGIVNNLPSLGAIENDLSNTISQIQNS
                     DILNQVVNQLQSA"
     misc_feature    18589..18604
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: strong, score: 30.52"
                     /tool="antismash"
     gene            18649..20727
                     /locus_tag="BW154_RS08690"
                     /old_locus_tag="BW154_08705"
     CDS             18649..20727
                     /EC_number="7.2.2.-"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015662 - P-type ion transporter activity
                     [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1037: heavy metal
                     translocating P-type ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905590.1"
                     /locus_tag="BW154_RS08690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW154_08705"
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="WP_098393961.1"
                     /transl_table=11
                     /translation="MSNKTHSSNHEEMMSEHHHNHEMVDMKHENHEMHGMDNMHDMDNM
                     DMMNHGGHMMHMGDMSKKLKVAIILMIPLLLISPIAGFTILKFPGSEILQLILGTIIFF
                     YSGTPFFSGAKGELKSRKPAMMMLITMGITVAYAYSVYATIMSLNGHMGMNFWFELATL
                     IVIMLIGHLIEMKAIMGAGDALKDLASLVPKKAHLKSGKDVELSELKVGDLLLVKENEK
                     IPADGLILSEALVDESMITGESRAVNKKTNDLVYGGSLNQNQPFEMKVTTLGKDSFLNQ
                     VAELVKKAQAQKSNLENMADRVAGYLFYAALIVGIFSLIFWTVSSNFSFALLLAVSVFV
                     IACPHALGLAVPLVVSRLTSISAKNGLLIQNRTSLEKINTIKYALMDKTGTLTDGKFIV
                     RNVIDFTDETEILQIMAALEGSSTHPIAQSIVSAAKPLENLKVEAVENIPGVGIKGQVN
                     QNFYQIVNYKYLRENQLSYDEQKIAQYLDLGLTVSFLINEQQDILGFIALGDSPKADAK
                     AFINGLLAQGITPVMLTGDNKETAQKIASALNIPEFRAELKPEDKAEIVKEYQKKAGVL
                     FIGDGVNDSPALATATIGFAIGAGTSVAISTADVVLVNSNPSDVLDMINISKRMLRKMK
                     QNLWFGAGYNIIAIPVAAGILYPFTGIYIDPLIAAVLMSISTVIVSINAMGLRYDKK"
ORIGIN
        1 ttgtttcctg acccttcata agttacttta attccaaacc aattagccaa ttgctcaact
       61 tcttttttcg accaatcata catatcaggc atcacctgat ctttatcatt cgtcaaaagt
      121 aaaattcgtg aatttgctga aactttttca ccttcagcaa tcgattgagc agttactttt
      181 gcccctgtcc cgataataac cggactaagg acagtacggc gtaagctatc agcagtatct
      241 cctgaatctt tgcctttata attctcaatg gtcactttac cagctttagt atcagtatca
      301 gtatcagcac taactgataa atcattcttc attgcttcag cacttgttaa caatggattt
      361 gccacacgag caatatattt aagtgtccaa ctttctgtag gaatttttac attcatatag
      421 aaaataaaat caggattctt agcaggatac atcacaacag cagagtaaag gtaatcttga
      481 gaacctgtca tataaccacc cgttggtgag gcaatttggg ccgtccccgt tttaactgca
      541 gccggttcac cattgaccat aaataaagga ccagcagcaa gattttgctc agaatcgcca
      601 cttgttgaat aagatgttcc gtaaacaggg tcagtattta ctgttaacat caaatcacgc
      661 acaccacttg ctgcatcatt agagaccgga tgtccaataa cttctgcttt agaagtcaaa
      721 cttgtttttg tattcggatc tactactttg ctaacaatat gtggttcaag cattgttcca
      781 tttccagcaa tagccgtcca acctctaatc aattgaagtg gagtaaccgc aaccccttga
      841 ccaaatgaag attgaatttg cgtaacggca ttcgatgaag gaagggctcc tggattttca
      901 ccatctaaac cggctcttac tttaagacca aatttaaatt tattaagata acttccccat
      961 aaatcattac ccatgttcat ttcaatttta ctcattccaa tatttgaaga taaagcaaaa
     1021 ccttgagcat aggttactgt ttctggcaaa gtataacttt tattttctgt tacatcccag
     1081 tcattaatcg ttgtatcata aacttgaagt ttccgttggt aagtttcatt aagatttact
     1141 tttccactat caagcgctcc agccatcaag aaagttttaa atgttgaacc cggttcaaag
     1201 gctgattgtg agagcaaact gttccaagtg aaatactttt gtttctcagc gtcattgatc
     1261 gttgttgccg tataggttgg acgttgactt gttgctaaaa tctcaccggt atgagcatca
     1321 actaaagttc ctgaaagttg ttgtgctcca gtatccgtag ccgctttgtc catatatcct
     1381 tcaagatttc tttgaagttg tgcatcaagt gtagtataaa cgtcttgacc atttttaaca
     1441 ggttcaatga ctttagttgt tcctggaatt gggcgaccat agatatcttt ttgatatgtt
     1501 tctttaccat tttcaccact caaaacatta ttaaaggctt tttccaagcc catatcaccc
     1561 ttgagagctt gagtaccatt ttcatcttta ggacgagcaa taccaataaa ttgagaagca
     1621 aaatttccaa aaggatatga acgagaaata tttgccgtga aaccaagtcc aacaattttt
     1681 tcattctcag ctgctttttg aatctctttc attttttcaa gagagatatc actcccttta
     1741 gggccaaatt gaacttgttt caatttagaa ttgagctgct ttttaatcaa atcacggtca
     1801 attccaagtt tactattcaa gaaatctcca actttggtaa aatctttctt atcaatataa
     1861 agaggatttc cgttcgaatc aacctgtgtt ttatcaatta cagcataaat tgtataggta
     1921 cttgagtcta ccgcaatagc cgcacctgta cggtcatata ttgtcccacg cttcgcctga
     1981 accgttactg tgctttggta gttattcttg gccattgttg taagattggt tccaccgaca
     2041 tgattgacgg taattagcca gacaaaacga aatataaaaa ccgtaaacag cgcaatagct
     2101 aggaaaaaca ggctcttacc tactcgttta cgatttcttt ctgtactcca attcgttttt
     2161 cgggcatttc gtgatacttt tctaaaagga aagaaaatta gtttttttaa aaattttatc
     2221 atttcgttgc tttcaataca ttagaaggat tttgttttaa atcagcttta ttcgccgtat
     2281 caagcacttt agtacctgat gttaaatcta aaatttgttg atcatattca gtttttcgtg
     2341 ttgtttcttg agaaattttt gaacgtaagt ccattgtatt cccttgaact tcaagtgtct
     2401 tagtcttaac ataaagtaga ccaacagcca acactaaagc agcaactacc attgtcagat
     2461 agaatacctt ctcaacacca gaaaactttt taaacttact tgctagaacc tctggcgcta
     2521 aagtttccgt atcaatgaga taactatctt ttttcgccgc agtttctata tcgaagtatt
     2581 ctttaggctg tgctgccatc tttgcctcca ctcgtttatc ttactaaaga aatttaactt
     2641 actttcttca ctattgaaaa gagaaaagct aaatattgtt atttcattta tataagacat
     2701 ccaaaaacaa tcctcaagtg ggaaaaccct ctttattaga ccaacttgtt atttgattaa
     2761 tctctttgtt tttgcgctac tcttaatttt gcactatgag ctcgattatt aaattcgagt
     2821 tcttcttcgc ttgcaagaac tggtttgcga ttaaccaatt ttagtttagc ttccatatct
     2881 ttaggaagca tcggtaatcc cttaggaaca ttaactgtac tatattcttt gaaaattgtt
     2941 tttgttaaac gatcttccaa agaatgaaaa gtaattaccg aaattcgacc atcaactttt
     3001 aataaatcaa tggcttgttc gattgattct tccgctgctc caagttcatc atttacttca
     3061 atacgaatcg cttggaaaat gcgtttggct ggatgccctt ttttcttgag ctccttttga
     3121 ggtaatgcag attttattaa atccgcaagc tcaattgttg tctcaatagg tttaattttt
     3181 cttgcttgtt caatcttacg agcaatttgt ttagagaatt tatcttcgcc gtagcggaaa
     3241 aaaatgcgaa ctaatgcttc gtaaggataa tcatttacaa cttcataagc agaaagagtt
     3301 tgattttggt ccatccgcat gtctaatctt gcctcttttt tgtaagagaa accacgttga
     3361 ctatcatcga attgtgggct agaaactcct aaatcataaa gaattccatc aattttcgtg
     3421 actcctaatt cagctaaagc tgatttaaga tagcgaaaat tacttttaat caatgttact
     3481 ttatcttctg caagttgctc acttaagcgt acttttgcgt tttcatgagc agtttcatcc
     3541 tggtcaaatg aataaagatg accatcagtc agtttactaa gaagatactc actatgtcct
     3601 gctccaccta gtgtcgcatc aacataaatt ccatttggct ttacttcaag catatctacg
     3661 gtttcatgta gtaagactgt atcatgctta aatgctattg tttttggcaa gagcgccgtc
     3721 ctccttgtat acttacagtt tattatatca ctttttttct atttttgtag tagtttacat
     3781 gattatgtaa agattttttt aataaaaagg cttacatgaa ctgcatttgt atctattact
     3841 gacttctttg atgtcaattc aatttacaag ttgtgtaaac gtgacttttt atagtttaac
     3901 aattaatact aaaaatagac tgaaattaat cagtctattt tttcaaataa agaatttttt
     3961 ttatttgttt ttaattctcc tgtcgccatt acttctacaa gaattaaact caatactgca
     4021 atcattacta gtaaaaataa gaggtattga actaattgat agtgaccggt tatatatgtt
     4081 atttttaaag cctttgtgaa catttgatag aaaattaata ttccacttgt tccacctaac
     4141 aaaagtgata gtcctaaatt acttagacca tcatctctta agcgtcttac atgaatcgta
     4201 agttgtggga aaaatgttcc taagataaat aggaaaaaga ataaacgcag taccatacta
     4261 ccaataaaat tgtagccaaa cattatttgt tcaaattgat tgaaaagata aattaaaaac
     4321 agaaaaagaa taaaggtcaa tccccaaaac agttgcatcc accaaaaatt tcttcttgtc
     4381 gttcttccgc gaaattcaaa gtaatttttg aaatagtcct tgaatatgcc tgacaatgtt
     4441 tttgtctctg gtaaattatt aaattcttga gaagaattaa tcttgtattc ctcgaatttt
     4501 aaagccctag gtgaacttgg aagtaggtta agtaccaaga gcacaagagt tcctagccca
     4561 aggataaaat taagaaaaat aagtgcccaa ggtaaatcag catcatttaa tcttcgaaca
     4621 gttagagcaa tgaaggacaa taaacttcca aagaaatata ggttaaatac caattgaaag
     4681 aatgaagcaa aagtttgcca taaagtcgtt agttgataaa aatctaataa attaaaaaaa
     4741 tgataagctt taaacaagat aacaaatatt atgatatgag ttaataaggc tgaccaaaaa
     4801 tctttacgac tagtttttct attgaaagta aaagctccta gccaaaattt tttataagct
     4861 tgaaccatat ttttcctctc tatttttatg attattcata agtccttgaa tcatcttctt
     4921 cgtcaatgcc atatttagtt ggaaaaagac aaagaatata aataatgaaa ttgataaatc
     4981 caacaaaata acccattttt gctagaaaat cttgatgaat aaacattgta accagcccat
     5041 aaattgcagc tactccaaaa ataagagcaa tccaccaagg aaacctagca tcacgtaaac
     5101 gacgagctgt caaactgata ttaggaacaa gaatagctag accaatgaga gctgtaacaa
     5161 taactattac taatgctcca cccgtggggt tggcatataa gtctgtcacc tgttgaataa
     5221 tttctttttg tgataaagtt gcagattggc tcattatttt tccagctttg gggattgtca
     5281 ccataaagtt taaaatgctg agaatcgcaa atatgattga attagtcaag aaaacccacc
     5341 aataatccga acgatttgaa taagatttta aatctcctgc ttttttccaa tatttgatgt
     5401 atgctttaat cattgttatt cctctttact tataatatta tttactgaca gaaggtcagt
     5461 tatttattcc aaactttttt attaagtaaa ctcatatttt tattaaaaga ttatttaaac
     5521 attttttcaa tatttttatc tggaattacc cacattgaaa aaattaaggc tgaaccaaca
     5581 agcatgatta ctggagctat aaaataacct aataaaatgc tgacaatatt gagcacaaca
     5641 cttaggatat tttttctaat cgttgtctca gacgttttac gatgtccatt gattttcaat
     5701 aactctcttg tcaaaagtag ataaataaaa ttccctaaga aaaagataat cacatagcct
     5761 aattctggga taaatgaatt aggatagatt gatacccagt tagaaaaaat aggaataagt
     5821 gataagacaa aaagaaaact aatatttatc catagaactt ttccattaat tcttttgatg
     5881 ctttgaaaaa gatgatggtg attgacccag taaatagcaa gtataaaaaa acttacaaaa
     5941 tagacaccca gatgcggaag taaagcaata aaactttgta agctatctga atctggacgg
     6001 accaattcta aaactaagac agtcatgata attgcgatga cagcatccgt aaatgcttct
     6061 aaacgtgttt tactcatttt taactcctaa attataaaat acgacttcag tttttaaatt
     6121 acaaacctct cctttcagta ctatggttga tatctcatta taacatattg agtttaattc
     6181 ataaaagaac tttaacagaa gttcttaatg gtaaaaaatc taattttact tcataatatt
     6241 tcctttggag ataaaaagcc ccaataaatt gaggcttcaa atttataata aaaacaccct
     6301 tatcttatat aggagaaggc gtttttatct atggctctct gacttggaag ttggcacata
     6361 gtatataaag atattttagc ctttttcaaa aggcttgtca gtaacatttg ctaacaaatc
     6421 aaagaaattt atctatactt agttttttta atttattttt tatcttttga tttctaagaa
     6481 tttggatcag catttcgcat aacatacaga ttattcccgc acaggcacca acaataccta
     6541 cgaacaagaa aaaataattt tgattataat gctcataaat aaaaaaaaaa aaaaaaacag
     6601 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnncaat ttaattttaa cattgattat ttcgttatat acatatttgt
     6781 ggttacaatt ttacactgat tatgattttg tcatcaaaaa gataaaaagt cctgattaaa
     6841 aatcaagact ttttcttaat aatattcgat ttatttgctc aataaacgtt tagcctgagc
     6901 caaagcttct ttagttaaat cttcaccagc aagcattttc gcaatttcat taactcgttc
     6961 gtcaaagttt aattttctaa ctgtggaagt agtaatatca tcggttgaag ctttttcaat
     7021 ataaaattgt ctgtcggcaa cggccacaac ttgtggaagg tgagaaattg caagaacttg
     7081 cccatgtctc gcaattttat atattttact agcaatcgct tgagcaacac ggccagaaac
     7141 accagtgtca acctcgtcaa aaacaatggc tgttttattt tcgcgtcttg aaaagcttga
     7201 ttttatagcg agcattaaac gtgaaagttc accacccgaa gccgtttttg ccagtggttt
     7261 aaatccttct cccggattgg tttgaataaa gaattcaaca tgctcatttc catttgccga
     7321 aaactttgcc ccttcaaaac taactttgaa atcagctttt tccatataaa gctcagaaag
     7381 ttcgtgttta atatcttttt caacttcttt tgcaatttta tggcgcgctt cattaagttt
     7441 tttacttgct tcaatgagtt gaacttgtgc aactttaact gaattttcta gattttctga
     7501 gtccttttct gaacccgtca gttctgagag ttcaagttgg actttctcta aatagttcat
     7561 cacatcggac agttctgggc catatttttt cttcaaagta gtcaaggtca taatccggtc
     7621 ttcaatttgt aaaagttccg ctggattaaa ttctaaatca ctcatgattc tttgaatttg
     7681 attagcaact tcttctaata cataataact ttcagcggtc ttgtcagcaa gttcttgata
     7741 gtcattatca aagttactaa ttttatccag ttctgtcatt gtggttcgga tattattaag
     7801 acttgaataa tcattatctt catcatcaag cgctagataa gcactactaa gcgaatcagc
     7861 aatattttta atattattta acttttctcg gcgattaact aattgttcat cttcttcaag
     7921 attaatttcc gccgcttcaa tttcttcagc ctgaaattga aggatttcaa ttctttgagc
     7981 aaattctcgc tcattttttt gtcgtaaatt gagctgttga cgcaaatttt tgaagttttc
     8041 aaatttgagt ttgtaattat cttttatcgc atcaaaggct tcatcaccaa attcatcaag
     8101 taaacgaaga tggcttttcg gattcatcaa ttcttggcta tcgtgttgac catgaatatc
     8161 aactaataat tcacccactt gtcttagcgt agctaaatta accatttgac cattgattcg
     8221 acaaacggag cgaccattgg caaaaatttc acgtcttaaa ataatttcag aatctacttc
     8281 ttcaaagcca agttctaata gtaccgcctt caattctgga gttttatcaa agaaaaagag
     8341 accttctatt tcggctttgt ttgccccatg ccgcacaaaa tcactcgaag cacgcccacc
     8401 taataataaa gacattgcat caataattat tgattttccc gcaccagttt ccccagttaa
     8461 aatcgtcatt ccactctcaa aggatagatg aatttcttca ataattgcaa aatttttgat
     8521 tgaaatctct tgtaacatca aaatttcctt tctcaaaaat aaatgacaag gctattagta
     8581 aactaataac cttatctttt tcctactttg attctttttt actgacagct attaattcgc
     8641 tcaaaatatt cagcagaatt ttatcacaat attcttgtca gtaaatttaa aattctgtca
     8701 gtaaattata aagattttta gcatcttctt ccatttcaaa aataactaaa gcactattat
     8761 catcaataat gattgaaaaa atatcatcgc catattcttc caaaagataa tttttcacaa
     8821 gactcgttgt ccctggatta gcaagaatcc agagcatatt atctttgatt tttgttcccg
     8881 taattgcatc aaaagctaag gctttatgaa ggcttgactg taaaacttcc ttgttttttg
     8941 gtaaatcata acgataacca ccagattcag ctggcacctt gattagggca agtgatttaa
     9001 tatcgcggga aacggtcgct tgagtaacgt caattccatg agttaagagg gtattcacaa
     9061 gttcttcttg cgttttaatt tcgttttcac ggataaattg agcaataaag tttaatcggt
     9121 cttctctttt catagttctt cactttcttt ttgtaaaact gattcaatat taacctgctg
     9181 agcaatttct gcttttccat ctttaagtaa atggacaaga aattcaacgt ttccagcccc
     9241 acccttgatt ggcgaaaagg tcaaaccttt tactgaaaaa ccaagttgag tggctgtttt
     9301 taaaactttt tcaatagtca tttggtgaac tttagaatca cgaataattc cattttttcc
     9361 gacttgttca cgtcccgctt caaactgcgg tttaataagt gctgctactt cgccattttc
     9421 ttccaaaatt tcataaagtg gcggtagaat taaatctaaa gaaataaaac taacatctat
     9481 actagtaaat gacggtcttc cttgtccaaa gtcagctaaa acagcatttc ggaaattaaa
     9541 ttgttccata acgacaacgc gttcatctga gcgaattttc caagccaatt gattggttcc
     9601 aacatctaaa gcatagacta atttggctcc attttgaagc attacatcag taaaaccacc
     9661 agttgaagaa ccaatatcaa gacaagtttt tccattaatt tctaaatgga attctttaag
     9721 cgccttttct aattttaatc ctcctcgact aacatatctt aacttttcac ctttaagacg
     9781 aagttctgtt ccatcatcaa ttttctgacc aggtttatcg taacgttctc ctgatttgct
     9841 atcaacaact agaccagcca ttactcctct tttggcctgc tctcgtgttt caaacaaacc
     9901 ttgattcgtg gcaagtacgt caattctttc tttcatgtag atctttcttt atagtaatta
     9961 tacgtatttt tcttagaaat gtaactaata atttcttttt ttatttcact gccaattgct
    10021 caataatttt aaaaatttca gagctgtcag taactgataa ggctgtcagt aattttttta
    10081 cctctgacag attttctgtc agtgattttt ttgctccctc aagtcctaaa tgagcaacat
    10141 atgttgattt ttcctctaaa atatccttac ctggtgtttt tccaagctca gcaaaagttg
    10201 ctgtaacatc taaaatatcg tctcgaattt gaaaagccaa tcccaaaatt tgccccactt
    10261 gacgaagttt ttcaacttca tccgttgatt tttgagcaac aattccagca gctacgaaag
    10321 gaaaagttaa aagtcgccca gttttcaagc gatgaatctg ctcaatttca gctaatgtca
    10381 actcttttcc ttcaccagcc atatctaaga tttgacccgc aaccattcca taagaacctg
    10441 aagcaaaggc caattcacgc gtcaacgcta ctattatttc tgctgataaa tcagcagttg
    10501 aaaggataaa gaaagggtca aaaaataaag tatcaccagc taaaattgca gttgcctcgt
    10561 caaatttctt gtgattagtt aactgcccgc gacgataatc atcattatcc attgctggta
    10621 agtcatcatg gattaaagaa cccgtatgaa tcatctcaag agcggctgcc acatgataat
    10681 gagccttact caattctaaa ttaaaagcct ctaataggtt taaaaaaagg agagggcgaa
    10741 ttcgtttacc gcctgccaat aaggaatatt ttgctgattc ggctaagcca gttggaaatt
    10801 cagcgctttc ataaaattca gttaaaaagt cttctaactt tcgtatcttc gtatccatta
    10861 ccctaattat accaaaattc acggctaaaa accgtgatga tttgaggaaa ttttttattt
    10921 tttaaatccg ttccattttt ataagtgagc cttttgctca ttcattgcta atactcttgt
    10981 aatattttga cttgttcgct caacgttctt tgcaccgtca gccaaggcat ctgacaaaga
    11041 ttgtgcttga ctcaaaatac caaaaatagc ggtcattcct tcctcataaa gaacttctgc
    11101 tccatcccca atatatcctg ccaaagcaaa gcaaggtttc ttgtattttt ttgcaatttt
    11161 acagacgcca taaggtgctt taccaaattt tgtctgataa tccattccac cttcacctgt
    11221 aaatacataa tcagcttctt taattttatc ttctaaatat attgtttgtg caacaagctc
    11281 aattcctaat tgcatttttg cattggtaaa agcaagcaaa cctgctccca aaccacctgc
    11341 tgcaccagct cccggtatat ttttaacatc ttttcctaga ttattatgaa caatttctgc
    11401 ataatgcttc aagttttcat ccaatacttt gaccatttcc tcagttgctc ccttttgtgg
    11461 tccaaaaaca taagaagcgc cattatttcc ggttaaagga ttggtgacat cagtagcaat
    11521 taatatttca atttcttcca ctcttgggtc aaaatcactc atatcaattg ttctgacttc
    11581 tttaaggaaa gctccaccaa gcggaatttc ttcttggtct ttatttaaaa acctagctcc
    11641 caaagcacga gccatgccag aaccaccatc attagtcgca ctcccaccaa tgccaatgat
    11701 gattttatct actccttcat ccattgcggc cttaattaat tgtcctgttc cataggttga
    11761 tgtaatcaat ggatttctat cttccaaagc aactaattcc agaccatttg cctttgccat
    11821 ctcaacgacc gccgtatttg agtttcccaa aataccaaaa taggcctcta catttgtatt
    11881 tgcaaatgaa ccctgtactt ttttatatac ccgtcgtcca cccgttgcat caatcaaagc
    11941 atctgttgtt ccctcaccac catcagccat tggcatagaa attatttcag cctcaggaaa
    12001 tactttttta attccttttt ccattgcttg acaaacttcc agcgcactca aactttcttt
    12061 gaaagaatct ggcgctaata caaatttttt cttcatttaa tctactcttt ctataaaaga
    12121 cttgaccaca actctattta ctattagtat ataataaata agattgatat tagaataata
    12181 ggcttttttg tcagtaaaat tttgtttaaa ataaaaaacc ttgaaaaatc aaagtttttt
    12241 tattttagta gtctttctgt tctggagaga aatcactctc ctgatcattt tttccaacta
    12301 tttttactaa tgtttcttcc gcttcattga gtgttttttt caaagtttcc gaaattttca
    12361 ttcctttttg aaattcagca atagcatctt ccaacgctac atcaccagac tctaacttac
    12421 gaacaatatt ttctaactct gccaaattat cttcaaattt tacatcttct ttttttgttg
    12481 ccattatttt acctccacaa ttacttttcc gtcagtcaat tcaacatcta aagtctgacc
    12541 ttttttcaca tcacttacgg atttaataat ctttcccttt tcgtcagtaa cgagggaaaa
    12601 accacgtgct tttatttttg agatatctaa taaaagtaaa gactgatata gttgctccgt
    12661 cttattcttc ttagcttcaa ctatctttcc gtaagtcgga attaatttcc cagctgacag
    12721 ctcataacgg tgtttcatat ttgcaacttt attttccgtc agcactgaca aacgttcaca
    12781 cagacggtca agtttttgta agtgtccatc gtacaagcgt tctggctgtc taaaaacgac
    12841 tgactggctt aatttatcta ctcgttcacg ccttatttta attaaatgtg ttaagcggtt
    12901 aaaaagtcgt ttttcctgct cattcgccca atttattaaa tcaaccttcg tatttggtgt
    12961 tgctaattcg gctgccgctg ttggagtcgc tgcacgacta tcagcaacaa aatcagctaa
    13021 agtcacatct gtttcatgtc caaccgatga aataattgga atacgtgatt caaaaattgc
    13081 tcgaaccaca atttcttcat taaatcccca taaatcttcg atagaaccac caccacgacc
    13141 aataatcatg acatcaaaat caccacgttc attagctcga cggatatttt cagcaatttc
    13201 ttcagccgaa ccttgtcctt gaacttttgt cggataaaga acaatttgac tcatcggaaa
    13261 acgacgttga accgtcgtaa taatatctcg aatgaccgct cctgacgggc tagttaccac
    13321 agctattttc ttagaaaatt gtggcaaagt ttgtttccaa cgttgttcaa atagcccttc
    13381 agcagtcagc tttttcttca actgttcaaa cttaaccgca agagctccaa cgccatctgg
    13441 aactaaactt tcaatattta tagaataaga gccagaaggt ggataaatac tgattcgacc
    13501 aacagccaaa actttcatcc cctcttccaa ttcaaagtct aatttgcgaa attgtcccgc
    13561 ccacatcgta gcttgaatca cagcaccctc atctttaagt gcaaaatatt ggtgatttgg
    13621 tcttcttcta aaatttgaaa tttctcctgt caaataaacc cgttcaagat aaggatcacg
    13681 gtcaaatttc gcttttaaat attttgttaa ggttgatact gataaatatt ctgtcattcc
    13741 ctcactcttt agttcttaat taaacttttt actaatcatt ctgttagtaa gatttctact
    13801 gctaaaatcg aaaaaaagta aaaagtttat cttcaaaaaa cttctaactt aaaaataagt
    13861 ccttagaaat tactataaac ttagttactc ttttattaat taactattta ttttcgttca
    13921 tttttctact tgctgcacga actgtctgtt ccatgagcat tgtaatcgtc atcggaccaa
    13981 ctccaccagg tactggggta ataaaacttg caacattttt tacttcatca aaatcaacat
    14041 caccatgaag ttttccatct tcatcacggt tcattccaac atcaattaca actgcacctt
    14101 ctttgatatc ctcagcttta atcatccggt cacggccaat agcaactacc aaaatatcag
    14161 cttccttagt caattcgcgc aagttttctg tttttgaatg agcaattgtc acagtagcat
    14221 cagccatcat taataattgc gccattggtt ttccgacgat atttgaacga ccaatcacaa
    14281 ctgcacgttt accagaaagt tccacattat attcacggaa catttccata ataccggctg
    14341 gagttgaagg aatcatcaga ggattcccag accacaaacg tcccatattc attgggtgaa
    14401 aaccatccac atctttttct ggatcaatag ctagtaaaac cttttcttca gaaatatgtt
    14461 ctggcaatgg caattgtacc aaaataccat gccattgttc tgattgatta tactgttcaa
    14521 ttaaatctaa cagttcttgt tctgtcactg tttctggtag acgaaccaca ctggaattta
    14581 atccaattgc tgtggcttgt cgtgctttat ttcggacata aatttgactc gcaggatctt
    14641 cgcccacaag aataacggca agacctggaa cattgtccgc ttcttttaat ttatcgactt
    14701 taactttcaa ctcagcttgc attttagcag caagtgcttt tccatctatc aagttcatag
    14761 aactattata ccataagcca acattcatcc tctaatcatt atcattcctt tttctttctt
    14821 tattttaaat tgttcgtaat ttttaataaa gaaataattc caaattaaaa agaaacttct
    14881 ttccaaagaa aagaagtcct taattaaaat atactactca aaaccttttt tatatttaac
    14941 ttttccaata gttaacgttg ccactaagcc taatcccaat tctgctaata ctaaacttaa
    15001 atcgtcatca gcacctgttt tgggaagttc ttctttcgtt acagttggta caagttgtaa
    15061 gctcgttgcg cttaatgaat ttgaaagact tggactactt gataccttag aaattaaagt
    15121 cgaaactttt gcttcattcg aaggtaaatt tgagtcactc aagttattta gtttcacttc
    15181 tgaagtatac gaactatttg aagttagact tgagccattt ttatcattag cttgtaaatt
    15241 tggattagtc aacatacttg aagatgttgt tgaaccactt gactcagatg aagtcaaact
    15301 tgcactactt gattcatttg aactcagact tgaatgactt gaattttcag aagagggact
    15361 tgtagtacta gtttgatcag tatttgaatt tgaatcactt atcccactag aatttgtaat
    15421 actactttcg cttgaactta aacttgaaac gcttgatccc tctgagcttg aatctgagct
    15481 acttacccca gctgaacttg agtgacttga gtccaccgaa gatgaactag cagtactact
    15541 ttcactggaa gttgaacttg aagaacttga gctctctgag cttgaatctg agctacttat
    15601 cccaacagaa cttgagtgac ttgagtccgc cgaagatgaa ctggcaatac cactttcact
    15661 ggaagttgaa cttgaaatgc ttgaactctc tgagcttgaa tctgagctac ttaccccagc
    15721 tgaacttgag tgacttgagt ccaccgaaga tgaactggca acactacttt cactggaatt
    15781 tgaacttgaa gtatttgaac cttctgagct tgaacttgag ccatttaccc cagctgaact
    15841 cgattgactt gagtctaccg aagatgaact agcaatacta ctttcactgg aagttgaact
    15901 tgaaatgctt gaactctctg agcttgaatt tgaactattt actccagcag aacttgtttg
    15961 acttgactct accgaggatg aacttgggac accactttca ctggaagttg aacttgaatc
    16021 attagaagtc gaacttgaag tatttgcttc actagaatta gaactcgtgt tgcttgatac
    16081 atttgaagat gaagttgaac tactagctgc acttgatgaa gaactggcac tactgcttga
    16141 ttggctacta ttagcactag aatttggatt aactgttgaa gcaccaacac ttggagttaa
    16201 agaattgata attgtcgtta ttccattaag tgcattatta acaaaattat taacattatt
    16261 ttgtatttga ctaatgatat tcgaaaggtc attttcgata gctccaagac ttggtaagtt
    16321 atttacaata cctgtcgcac tttcaacaat attattgata gcactttgca attgattgac
    16381 cacttggtta agaatatccg aattttgtat ttgactaatg gtattcgaaa ggtcattttc
    16441 gatagctcca agacttggta agttatttac aatccccgtc gcactttcaa caatattatt
    16501 aatagcactt tgcaattgat tgaccacttg gttaagaata tccgaatttt gtatttgact
    16561 aatggtattc gaaaggtcat tttcgatagc tccaannnnn nnnnnnnnnn nnnnnnnnnn
    16621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16681 nagcactttg caattgattg accacttggt taagaatatc cgaattttgt atttgactaa
    16741 tggtattcga aaggtcattt tcgatagctc caagacttgg taagttattt acaatccccg
    16801 tcgcactttc aacaatatta ttgatcgcat tttgcaattg attgagcact tggttaagaa
    16861 tatccgaagc accagcagga agattaccaa gtgagtttag actattttgg agcgtgttct
    16921 caatcgtggc taaggctgtt gaaccaaggc ttgtaagatt acccaaacta ttatttactt
    16981 ggtcaagaag gctggaaact cccgttaaat tcgcaacttc tcccaaagat gctccagtaa
    17041 gattatttaa tattgggctt aaaacattat taactgtagt acctacaaga ggaatattac
    17101 taatagttgg agataattga ctccaagcaa tttgacctgc tcgattaatt gctgaattaa
    17161 ctactaaacc agccaagtag ttgacaagat catttccttg aagagagaca agtgtgtcta
    17221 tatcaaaatt aacaccaccc aaggcattgg aagttgtcgt attaagttga ttaacaatcc
    17281 cacttgttac agtacttatt gcactaagta ctgggcttag tactagattt gcccccggaa
    17341 tacttgaaag tcctggaatt gttggatttt caagaaaatt aagcatagca gcagggctca
    17401 ttgatgggat ggcattaaat gccgtcacaa tctgccctcc aactaatgga atttggctaa
    17461 catctaaacc tgcagatttc attccattta atagaaaggc cccaggatta gcagcagctt
    17521 cctgaatatt tccaacaata ttgacaattt gttcaacacc attaagagct gacgcggctg
    17581 tagtcattcc tggaatatta aaagttccaa gagtgtttaa aatattttct aaaccactaa
    17641 ttcccactgt cgaaattgct tgatttacaa gatctccgag tgttgtgtta agaatagctg
    17701 aaccacttgt tgtagcaggc gctgctgcag ctagtgcctg agtaagcatt ggtaccacgg
    17761 acgaattagt cgctagaata gctgtaaaag aagcaacagc agcacttgaa ccattagcac
    17821 tactattttc cgcaagattt atttccgaac tttgagaagt tgatccaata ccactatcaa
    17881 ttgctgaact ttcactcaaa attactgatg aacttgttga actttgagag acagctgccg
    17941 aatcaacgct gtctgcagca ttagaagaac ttaatataga attgctgata ttatcatttg
    18001 atgagcttga atcagcaagt gatacaatat tgttcgtagc agtttgaata accgctgtac
    18061 ttatcgtatt ttggctttca ttaattgagt tgctactatc tgaaacatct gaagtcattc
    18121 cctcagtatc agtttctact gtgttagcca gtgaattatt agaactttgg tcactagctt
    18181 gttcgtaagc aacttgattt tgactagcag caacatcact atttgtcgac gaagctagtg
    18241 ctgaataact tgtttcttct gtaataccac taatttgctg actactatca actgctgtgt
    18301 ccgcttttac attttggtca gtaatcgttc ctgaaacgat aattaaagct aatgctgaag
    18361 tagcaaataa ccaacttttc cctgatttcc aggttctaaa atgggacttg gagataacag
    18421 tagacacttc tggccgtatt tttttcattt attctctcct tgctgatact ttttataaag
    18481 tatcactatg attatatatc atgatatcac atttaagtta caaaagaaac ctttctgttg
    18541 ttatttcgta acatttatag aatacaactt ttttccttga ctttgatagt ttacaaatgt
    18601 aaactatata tagatagaaa tttcaaagca ttcatttagg aggaaaatat gtcaaataaa
    18661 actcattcgt ctaatcacga agaaatgatg tctgagcatc atcacaatca tgaaatggtg
    18721 gatatgaagc acgaaaacca tgagatgcat ggcatggata atatgcatga tatggacaat
    18781 atggatatga tgaatcatgg aggacacatg atgcatatgg gtgatatgag taaaaagctc
    18841 aaagtggcta tcattctaat gattcccctt ctactcattt caccaatcgc tggctttact
    18901 atccttaaat tccccggaag tgaaattttg caactcatcc ttggtacaat tatcttcttt
    18961 tactctggga ctcctttttt tagcggagca aaaggtgaat taaaaagccg taagccagcc
    19021 atgatgatgc tgattacaat gggaattacc gttgcctatg cttattctgt ttatgccaca
    19081 atcatgagtc ttaacggaca tatgggcatg aacttctggt ttgaactggc aactttaatt
    19141 gtcattatgt tgattggtca cctgattgag atgaaagcaa tcatgggtgc tggcgatgca
    19201 ttgaaagatt tagcaagcct tgttcccaaa aaagctcact taaaaagtgg gaaggatgtg
    19261 gagctttcag aactaaaagt tggcgattta cttttagtga aagaaaatga gaaaatacct
    19321 gcagatggcc ttatattaag tgaagcgctc gttgatgagt caatgattac tggtgaaagc
    19381 cgagcagtca ataaaaaaac aaatgacctt gtttatggtg gttccctcaa tcaaaaccaa
    19441 ccatttgaga tgaaagtcac aacccttgga aaagattcat ttctcaatca agtggccgaa
    19501 ttagtcaaga aagcccaagc tcaaaaatct aatttggaaa atatggctga cagagtggct
    19561 ggttaccttt tctatgctgc acttattgtc ggtatcttct cattaatttt ttggacagtt
    19621 agctctaact ttagttttgc tctacttctt gcggtttctg tttttgtaat tgcatgtcca
    19681 cacgctttag gtcttgcggt tccacttgtt gtttctcgct taaccagtat ttctgctaaa
    19741 aatggtttat taattcaaaa cagaacttct ctagaaaaaa taaatacaat taaatatgcc
    19801 ttaatggata aaacaggaac attaactgac ggtaagttta ttgttcgtaa tgttattgat
    19861 tttactgatg aaacagaaat tctccaaatc atggcagcac ttgaaggaag ttccactcac
    19921 ccgattgctc aatcaattgt ttcagctgct aaacctcttg aaaatctaaa agttgaagcg
    19981 gttgaaaata ttccaggagt tgggattaaa gggcaagtta atcaaaactt ttatcaaatt
    20041 gttaactata aatatcttcg tgaaaatcaa ctttcttatg atgaacaaaa gattgctcaa
    20101 tatttagatt taggtttaac tgtatctttc ctaatcaatg agcaacaaga tattttagga
    20161 tttattgcct taggagattc tcctaaagca gacgcgaaag cctttattaa tggtttatta
    20221 gcccaaggaa ttacccctgt tatgctcacg ggtgataata aagaaactgc tcaaaaaatt
    20281 gctagtgcac taaatattcc cgaatttaga gcggaattaa aacctgaaga taaagcagaa
    20341 attgtgaaag agtaccaaaa gaaagccggc gtgcttttca ttggtgatgg tgtcaatgat
    20401 tccccagctc ttgcaactgc tacgattggt tttgcgattg gagcaggaac ttcggttgct
    20461 atcagtaccg ctgatgtcgt cctagttaac tccaatccaa gtgacgtctt ggatatgatt
    20521 aatatctcca aacgcatgct tagaaaaatg aaacaaaacc tctggtttgg agcaggctat
    20581 aacattattg ctattcctgt tgcagcgggt attctttatc catttacagg gatttatatt
    20641 gaccctctca ttgcagcagt cctgatgtca atctcaaccg tgattgtctc aatcaacgcg
    20701 atgggattga ggtatgataa aaaatagaag agcctatgct cttctatttt tttcatacta
    20761 attatgaaac ttttatcact taaatatact taaagattaa gtatgatatc acacaaatac
    20821 taatgattgt aatgatattt tttaaatatg atttattt
//
