GCF_002563455.1 - 6 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_MTJR01000006.1 (Lactococcus lactis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 3.1	terpene-precursor	167,812	188,669
Region 3.2	T3PKS	879,534	920,688

NZ_MTJR01000004.1 (Lactococcus lactis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 6.1	RiPP-like	1,437	11,604

NZ_MTJR01000005.1 (Lactococcus lactis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 7.1	betalactone	103,876	136,407
Region 7.2	lanthipeptide-class-i	137,516	163,547	High	nisin A	ribosomal:RiPP:Lanthipeptide

NZ_MTJR01000009.1 (Lactococcus lactis)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 9.1	RaS-RiPP	1	22,619

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 3.1	terpene-precursor	167,812	188,669
Region 3.2	T3PKS	879,534	920,688
Region 6.1	RiPP-like	1,437	11,604
Region 7.1	betalactone	103,876	136,407
Region 7.2	lanthipeptide-class-i	137,516	163,547	High	nisin A	ribosomal:RiPP:Lanthipeptide
Region 9.1	RaS-RiPP	1	22,619

No secondary metabolite regions were found in these records:: NZ_MTJR01000007.1 (Lactococcus lactis); NZ_MTJR01000003.1 (Lactococcus lactis); NZ_MTJR01000012.1 (Lactococcus lactis); NZ_MTJR01000001.1 (Lactococcus lactis); NZ_MTJR01000008.1 (Lactococcus lactis); NZ_MTJR01000011.1 (Lactococcus lactis); NZ_MTJR01000010.1 (Lactococcus lactis)

Compact view

NZ_MTJR01000006.1 - Region 1 - terpene-precursor

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Location: 167,812 - 188,669 nt. (total: 20,858 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

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other genes

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Terpene

TFBS Finder

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: synthetase

BW153_RS04150: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.39	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0001756.3	0.29	terpene	retigeranin, arathanatriene	Arabidopsis thaliana
BGC0001605.3	0.28	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0001227.3	0.27	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.27	terpene	carotenoid	Streptomyces avermitilis
BGC0000648.3	0.27	terpene	carotenoid	Myxococcus xanthus
BGC0001529.5	0.27	other (other)	benzastatin J, 7-hydroxyl benzastatin B, 7-hydroxyl O-demethylbenzastatin A, JBIR-67, 7-hydroxyl benzastatin F, 7-hydroxyl O-demethylbenzastatin D, O-demethylvirantmycin, 7-hydroxyl benzastatin D, virantmycin, benzastatin K, methylbenzastatin K	Streptomyces sp. RI18
BGC0001456.3	0.27	terpene	isorenieratene	Streptomyces argillaceus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.39	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0001756.3	0.29	terpene	retigeranin, arathanatriene	Arabidopsis thaliana
BGC0001605.3	0.28	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0001227.3	0.27	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.27	terpene	carotenoid	Streptomyces avermitilis
BGC0000648.3	0.27	terpene	carotenoid	Myxococcus xanthus
BGC0001529.5	0.27	other (other)	benzastatin J, 7-hydroxyl benzastatin B, 7-hydroxyl O-demethylbenzastatin A, JBIR-67, 7-hydroxyl benzastatin F, 7-hydroxyl O-demethylbenzastatin D, O-demethylvirantmycin, 7-hydroxyl benzastatin D, virantmycin, benzastatin K, methylbenzastatin K	Streptomyces sp. RI18
BGC0001456.3	0.27	terpene	isorenieratene	Streptomyces argillaceus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

BW153_RS04150: PT_FPPS_like

Domain 1 (25 - 281): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: DMAPP, IPP → FPP

Transcription Factor Binding Sites

Binding sites found:

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 30.52 out of 30.97

Confidence: strong

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 24.74 out of 28.14

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.27 out of 26.06

Confidence: strong

Additional weak hits (4):

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 25.66 out of 33.14

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.48 out of 19.52

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 19.12 out of 30.11

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 23.05 out of 33.73

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CopR	Copper-responsive regulator
MogR	Repressor of flagellar motility genes
PerR	Peroxide regulator

NZ_MTJR01000006.1 - Region 2 - T3PKS

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Location: 879,534 - 920,688 nt. (total: 41,155 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

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Gene overview

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MIBiG comparison

KnownClusterBlast

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TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase reductase

BW153_RS07560: dehydrogenase

smcogs: SMCOG1181: dehydrogenase

BW153_RS07645: hydroxymethylglutaryl-CoA reductase

smcogs: SMCOG1187: hydroxymethylglutaryl-CoA reductase

Transferases: acetyltransferase

BW153_RS07650: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

BW153_RS07690: Chloramphenicol acetyltransferase

smcogs: SMCOG1156: Chloramphenicol acetyltransferase

Ligases: synthase

BW153_RS07655: hydroxymethylglutaryl-CoA synthase

smcogs: SMCOG1043: hydroxymethylglutaryl-CoA synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000866.5	0.51	other (other)	polyhydroxyalkanoate	Burkholderia sp. DSM 9242
BGC0000867.3	0.49	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0002561.3	0.40	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0001080.5	0.37	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0000286.3	0.36	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0000934.4	0.36	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0001076.5	0.35	other (nucleoside)	liposidomycin B, liposidomycin A, liposidomycin C, liposidomycin Z, liposidomycin H, liposidomycin G, liposidomycin L, liposidomycin M, liposidomycin K, liposidomycin N	Streptomyces sp. SN-1061M
BGC0000872.5	0.35	other (other)	A-90289 A, A-90289 B	Streptomyces sp. SANK 60405
BGC0001078.5	0.35	PKS, terpene	furaquinocin B	Streptomyces sp. KO-3988
BGC0002525.3	0.34	PKS	fusarubin, 1233A, 1233B, NG-391, lucilactaene	Fusarium sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000866.5	0.51	other (other)	polyhydroxyalkanoate	Burkholderia sp. DSM 9242
BGC0000867.3	0.49	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0002561.3	0.40	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0001080.5	0.37	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0000286.3	0.36	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0000934.4	0.36	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0001076.5	0.35	other (nucleoside)	liposidomycin B, liposidomycin A, liposidomycin C, liposidomycin Z, liposidomycin H, liposidomycin G, liposidomycin L, liposidomycin M, liposidomycin K, liposidomycin N	Streptomyces sp. SN-1061M
BGC0000872.5	0.35	other (other)	A-90289 A, A-90289 B	Streptomyces sp. SANK 60405
BGC0001078.5	0.35	PKS, terpene	furaquinocin B	Streptomyces sp. KO-3988
BGC0002525.3	0.34	PKS	fusarubin, 1233A, 1233B, NG-391, lucilactaene	Fusarium sp.

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.21 out of 26.06

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.92 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.96 out of 26.06

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.26 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.93 out of 26.06

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.41 out of 27.78

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.76 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.30 out of 26.06

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.00 out of 27.78

Confidence: medium

Additional weak hits (4):

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 22.66 out of 26.12

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 25.35 out of 36.54

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 24.42 out of 31.73

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 23.72 out of 32.93

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

TFBS Finder

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
MogR	Repressor of flagellar motility genes

NZ_MTJR01000004.1 - Region 1 - RiPP-like

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Location: 1,437 - 11,604 nt. (total: 10,168 nt) Show pHMM detection rules used

Region on contig edge.

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BW153_RS00315: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000507.5	0.29	ribosomal	epicidin	Staphylococcus epidermidis
BGC0000631.5	0.28	terpene	Botrydial, 8,9-epibotrydial, 1-epibotrydial, Dihydrobotrydial, 10-Oxo-dihydrobotrydial, 10-dehydroxy dihydrobotrydialone, 10-epi-dihydrobotrydial, Botryendial, Botryenalol, Botrydienal, Norbotrydialone acetate, 10-Oxodihydrobotry-1(9),4(5)-diendial, 10-Oxodehydrodihydrobotrydial, Dehydrobotrydienol, 11-Hydroxydehydrobotrydienol, 12-hydroxydehydrobotrydienol, Secobotrytriendiol, Secobotrytriene-10,12,15-triol, Secobotrydiene-3,10,15-triol, Secobotrydiene-3,4,10,15-tetraol, 4β-Acetoxytetrahydrobotryslactone	Botrytis cinerea B05.10
BGC0001547.3	0.25	terpene	citreohybridonol	Aspergillus stellatus
BGC0000188.5	0.24	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0001591.4	0.23	other (other)	fatty acid enol ester, N-tetradecanoyl tyrosine	uncultured bacterium CSLC2
BGC0002002.3	0.23	NRPS	1,6-phenazinedimethanol, fontizine A	Serratia fonticola
BGC0000297.5	0.22	NRPS, PKS, saccharide	aeruginoside 126B, aeruginoside 126A	Planktothrix agardhii NIVA-CYA 126
BGC0000259.3	0.22	PKS	prodigiosin	Serratia marcescens
BGC0002614.2	0.21	NRPS, PKS	subglutinol A, sesquicillin A, colletotrichin, metarhizin A	Penicillium shearii
BGC0002495.2	0.21	NRPS	photobactin	Photorhabdus luminescens

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000507.5	0.29	ribosomal	epicidin	Staphylococcus epidermidis
BGC0000631.5	0.28	terpene	Botrydial, 8,9-epibotrydial, 1-epibotrydial, Dihydrobotrydial, 10-Oxo-dihydrobotrydial, 10-dehydroxy dihydrobotrydialone, 10-epi-dihydrobotrydial, Botryendial, Botryenalol, Botrydienal, Norbotrydialone acetate, 10-Oxodihydrobotry-1(9),4(5)-diendial, 10-Oxodehydrodihydrobotrydial, Dehydrobotrydienol, 11-Hydroxydehydrobotrydienol, 12-hydroxydehydrobotrydienol, Secobotrytriendiol, Secobotrytriene-10,12,15-triol, Secobotrydiene-3,10,15-triol, Secobotrydiene-3,4,10,15-tetraol, 4β-Acetoxytetrahydrobotryslactone	Botrytis cinerea B05.10
BGC0001547.3	0.25	terpene	citreohybridonol	Aspergillus stellatus
BGC0000188.5	0.24	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0001591.4	0.23	other (other)	fatty acid enol ester, N-tetradecanoyl tyrosine	uncultured bacterium CSLC2
BGC0002002.3	0.23	NRPS	1,6-phenazinedimethanol, fontizine A	Serratia fonticola
BGC0000297.5	0.22	NRPS, PKS, saccharide	aeruginoside 126B, aeruginoside 126A	Planktothrix agardhii NIVA-CYA 126
BGC0000259.3	0.22	PKS	prodigiosin	Serratia marcescens
BGC0002614.2	0.21	NRPS, PKS	subglutinol A, sesquicillin A, colletotrichin, metarhizin A	Penicillium shearii
BGC0002495.2	0.21	NRPS	photobactin	Photorhabdus luminescens

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 24.68 out of 28.14

Confidence: medium

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 30.52 out of 30.97

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 24.06 out of 27.78

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.95 out of 26.06

Confidence: medium

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 24.10 out of 32.93

Confidence: medium

TFBS Finder

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
CopR	Copper-responsive regulator
LexA variant 3	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes
PerR	Peroxide regulator

NZ_MTJR01000005.1 - Region 1 - betalactone

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Location: 103,876 - 136,407 nt. (total: 32,532 nt) Show pHMM detection rules used

betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: carboxylase

BW153_RS02895: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

Ligases: ligase

BW153_RS02860: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002116.3	0.41	ribosomal	darobactin A	Photorhabdus khanii HGB 1456
BGC0000501.3	0.40	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0001579.4	0.39	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0001391.3	0.37	ribosomal	thusin	Bacillus thuringiensis serovar rongseni
BGC0000412.5	0.36	NRPS	pyochelin	Pseudomonas aeruginosa PAO1
BGC0000904.5	0.35	other (other)	FR-900098	Streptomyces rubellomurinus
BGC0002092.3	0.34	NRPS	stechlisin B2, stechlisin C3, stechlisin D3, tensin, stechlisin E2, stechlisin F	Pseudomonas sp.
BGC0000497.3	0.34	ribosomal	BHT-Aα, BHT-Aβ	Streptococcus ratti
BGC0002681.3	0.34	other (other)	rhizoferrin	Francisella tularensis subsp. tularensis SCHU S4
BGC0002699.2	0.34	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002116.3	0.41	ribosomal	darobactin A	Photorhabdus khanii HGB 1456
BGC0000501.3	0.40	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0001579.4	0.39	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0001391.3	0.37	ribosomal	thusin	Bacillus thuringiensis serovar rongseni
BGC0000412.5	0.36	NRPS	pyochelin	Pseudomonas aeruginosa PAO1
BGC0000904.5	0.35	other (other)	FR-900098	Streptomyces rubellomurinus
BGC0002092.3	0.34	NRPS	stechlisin B2, stechlisin C3, stechlisin D3, tensin, stechlisin E2, stechlisin F	Pseudomonas sp.
BGC0000497.3	0.34	ribosomal	BHT-Aα, BHT-Aβ	Streptococcus ratti
BGC0002681.3	0.34	other (other)	rhizoferrin	Francisella tularensis subsp. tularensis SCHU S4
BGC0002699.2	0.34	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 25.90 out of 27.78

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 19.05 out of 20.97

Confidence: strong

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 25.98 out of 27.78

Confidence: strong

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.20 out of 26.33

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.76 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.11 out of 26.06

Confidence: medium

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 25.74 out of 30.25

Confidence: medium

Additional weak hits (2):

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 23.17 out of 35.63

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 22.59 out of 29.25

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
ColR	Phenol-responsive regulator
MogR	Repressor of flagellar motility genes

NZ_MTJR01000005.1 - Region 2 - lanthipeptide-class-i

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Location: 137,516 - 163,547 nt. (total: 26,032 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: protease

BW153_RS03005: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: cyclase dehydratase

BW153_RS02985: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

BW153_RS02995: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

BW153_RS03040: diguanylate cyclase

smcogs: SMCOG1025: diguanylate cyclase

Ligases: synthase

BW153_RS02945: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000535.4	0.96	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0000538.3	0.93	ribosomal	nisin Z	Lactococcus lactis
BGC0000536.3	0.86	ribosomal	nisin Q	Lactococcus lactis
BGC0001789.3	0.78	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000537.3	0.76	ribosomal	nisin U	Streptococcus uberis
BGC0000549.3	0.68	ribosomal	salivaricin D	Streptococcus salivarius
BGC0000542.3	0.59	ribosomal	paenicidin B	Paenibacillus terrae
BGC0002323.2	0.58	ribosomal	nisin J	Staphylococcus capitis
BGC0000541.5	0.57	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000506.4	0.56	ribosomal	entianin	Bacillus subtilis subsp. spizizenii

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000535.4	0.96	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0000538.3	0.93	ribosomal	nisin Z	Lactococcus lactis
BGC0000536.3	0.86	ribosomal	nisin Q	Lactococcus lactis
BGC0001789.3	0.78	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000537.3	0.76	ribosomal	nisin U	Streptococcus uberis
BGC0000549.3	0.68	ribosomal	salivaricin D	Streptococcus salivarius
BGC0000542.3	0.59	ribosomal	paenicidin B	Paenibacillus terrae
BGC0002323.2	0.58	ribosomal	nisin J	Staphylococcus capitis
BGC0000541.5	0.57	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000506.4	0.56	ribosomal	entianin	Bacillus subtilis subsp. spizizenii

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BW153_RS02985

BW153_RS02980

Leader:

MSTKDFNLDLVSVSKKDSGASPR

Core:

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

Core with dehydrations:

IDhbDhaIDhaLCysDhbPGCysKDhbGALMGCysNMKDhbADhbCysNCysDhaIHVDhaK

antiSMASH-DB 4.0 matches for BW153_RS02980

100.0%

NC_013656.1and 18 others:

NC_020450.1 Lanthipeptides: LILO_RS03015_lanthipeptideNZ_CP009472.1 Lanthipeptides: LG36_RS03420_lanthipeptideNZ_CP010050.1 Lanthipeptides: QI18_RS03300_lanthipeptideNZ_CP015903.1 Lanthipeptides: LLUC08_RS03255_lanthipeptideNZ_CP024954.1 Lanthipeptides: CV702_RS02990_lanthipeptideNZ_CP066300.1 Lanthipeptides: LacL0098_RS03205_lanthipeptideNZ_CP098790.1 Lanthipeptides: M5C73_RS03405_lanthipeptideNZ_JAALEQ010000006.1 Lanthipeptides: HFD15_RS10120_lanthipeptideNZ_JAJONJ010000005.1 Lanthipeptides: LSG16_RS07195_lanthipeptideNZ_JAQDWM010000007.1 Lanthipeptides: PGU01_RS08180_lanthipeptideNZ_JAQDXV010000018.1 Lanthipeptides: PGT80_RS11850_lanthipeptideNZ_JAQEIZ010000019.1 Lanthipeptides: PGX18_RS12025_lanthipeptideNZ_JAQEOU010000022.1 Lanthipeptides: PGV42_RS12330_lanthipeptideNZ_JAQEOV010000015.1 Lanthipeptides: PGW09_RS10765_lanthipeptideNZ_MTJP01000006.1 Lanthipeptides: BW151_RS03795_lanthipeptideNZ_MTJR01000005.1 Lanthipeptides: BW153_RS02980_lanthipeptideNZ_MTJS01000002.1 Lanthipeptides: BW154_RS09850_lanthipeptideNZ_RAGL01000022.1 Lanthipeptides: D6108_RS11395_lanthipeptide

Lanthipeptides: LLKF_RS13210_lanthipeptide

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

34

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

34

97.1%

NZ_CP065984.1and 2 others:

NZ_CP095737.1 Lanthipeptides: MXM90_RS03205_lanthipeptideNZ_JAQDXJ010000021.1 Lanthipeptides: PGU19_RS11740_lanthipeptide

Lanthipeptides: I6I21_RS02785_lanthipeptide

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

34

ITSISLCTPGCKTGALMGCNMKTATC+CSIHVSK

1

ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK

34

97.1%

NZ_JAQDYA010000007.1and 1 other:

NZ_JAQDYA010000007.1 Lanthipeptides: PGT72_RS09560_lanthipeptide

Lanthipeptides: PGT72_RS09560_lanthipeptide

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

34

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVS+

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSR

34

with 123 more sequence matches

Only the first 10 are displayed here.

85.3%

NZ_FBTD01000003.1:

Lanthipeptides: PB1E_RS03075_lanthipeptide

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

34

ITS+SLCTPGCKTGALMGCNMKTA+C C +HVSK

1

ITSVSLCTPGCKTGALMGCNMKTASCGCHVHVSK

34

82.4%

NZ_JADNFO010000025.1:

Lanthipeptides: I2C89_RS09745_lanthipeptide

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

34

ITS SLCTPGCKTG LM C++KTATCNCSI VSK

1

ITSYSLCTPGCKTGVLMACHLKTATCNCSIIVSK

34

79.4%

NZ_CP090412.1and 3 others:

NZ_QSTB01000004.1 Lanthipeptides: DXC22_RS03785_lanthipeptideNZ_VSTZ01000009.1 Lanthipeptides: FYK98_RS07445_lanthipeptideNZ_VSTZ01000009.1 Lanthipeptides: FYK98_RS07445_lanthipeptide

Lanthipeptides: LZF92_RS09890_lanthipeptide

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

34

ITS SLCTPGCKTGALMGC MKTA+C C +H+SK

1

ITSYSLCTPGCKTGALMGCTMKTASCGCHVHISK

34

77.1%

NZ_FNDP01000013.1:

Lanthipeptides: BLP54_RS11230_lanthipeptide

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHV

32

ITS SLCTPGC TG LMGCN KTATCNCS+HV

3

ITSKSLCTPGCVTGVLMGCNNKTATCNCSVHV

34

70.6%

NC_022237.1and 1 other:

NZ_JUZI01000019.1 Lanthipeptides: ADI02_RS09325_lanthipeptide

Lanthipeptides: SII_RS05025_lanthipeptide

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

34

+TS+ +CTPGC TGALMGCN++TATCNC +H+SK

1

VTSVFMCTPGCVTGALMGCNLQTATCNCHVHISK

34

68.6%

NZ_CP013655.1and 2 others:

NZ_CP013655.1 Lanthipeptides: ATZ35_RS03635_lanthipeptideNZ_CP013655.1 Lanthipeptides: ATZ35_RS03630_lanthipeptide

Lanthipeptides: ATZ35_RS03640_lanthipeptide

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

34

ITS SLCTPGC TG LMGC +KT TCNCS+ + K

1

ITSKSLCTPGCVTGVLMGCALKTITCNCSVGIGK

34

68.6%

NZ_FNDP01000013.1and 1 other:

NZ_FNDP01000013.1 Lanthipeptides: BLP54_RS11225_lanthipeptide

Lanthipeptides: BLP54_RS11220_lanthipeptide

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHV

32

ITS SLCTPGC TG LMGC +ATCNCS+HV

3

ITSKSLCTPGCVTGLLMGCAGSSATCNCSVHV

34

67.6%

NZ_CP059471.1and 3 others:

NZ_FNZW01000001.1 Lanthipeptides: BMZ61_RS02110_lanthipeptideNZ_FOYC01000001.1 Lanthipeptides: BMZ88_RS03370_lanthipeptideNZ_KE384124.1 Lanthipeptides: G597_RS0106700_lanthipeptide

Lanthipeptides: H1R75_RS06635_lanthipeptide

1

ITSISLCTPGCKTGALMGCNMKTATCNCSI

30

ITS SLCTPGCKTGALM C +KTATC C

1

ITSKSLCTPGCKTGALMTCPIKTATCGCHF

30

67.6%

NZ_LR793265.1:

Lanthipeptides: HV392_RS09830_lanthipeptide

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

34

ITS SLCTPGC TG LMGC ++A CN +HVSK

1

ITSYSLCTPGCITGVLMGCQFQSAGCNVHVHVSK

34

64.7%

NZ_CP009913.1and 1 other:

NZ_CP009913.1 Lanthipeptides: SSAL8618_RS00810_lanthipeptide

Lanthipeptides: SSAL8618_RS00815_lanthipeptide

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

34

ITS SLCTPGC TG LMGC++++ CN +HVSK

1

ITSYSLCTPGCITGVLMGCHIQSIGCNVHVHVSK

34

MIBiG 4.0 matches for BW153_RS02980

97.1%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

1

ITSISLCTPGCKTGALMGCNMKTATCNCSIHVSK

34

ITSISLCTPGCKTGALMGCNMKTATC+CSIHVSK

1

ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK

34

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 21.61 out of 25.88

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 16.78 out of 18.16

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.40 out of 19.26

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.62 out of 26.06

Confidence: medium

Additional weak hits (3):

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 20.28 out of 29.72

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 24.64 out of 35.63

Confidence: weak

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 15.72 out of 18.46

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

BW153_RS02980 - Class I

Cleavage pHMM score: 26.70

RODEO score: 27

Monoisotopic mass: 3310.6 Da

Molecular weight: 3313.0 Da

Number of bridges: 5

Alternative weights: (assuming N unmodified Ser/Thr residues); 3331.0 Da N = 1; 3349.0 Da N = 2; 3367.0 Da N = 3; 3385.1 Da N = 4

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 141 - 229; Score: 28.7

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CRP	Cyclic AMP receptor protein
MogR	Repressor of flagellar motility genes
PsrA	Oleic acid responsive regulator

NZ_MTJR01000009.1 - Region 1 - RaS-RiPP

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Location: 1 - 22,619 nt. (total: 22,619 nt) Show pHMM detection rules used

Region on contig edge.

RaS-RiPP: (PF04055 and TIGR01716)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BW153_RS11185: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001209.3	0.62	ribosomal	streptide	Streptococcus thermophilus LMD-9
BGC0001929.2	0.43	ribosomal	WGK	Streptococcus ferus
BGC0000902.4	0.42	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0000285.5	0.37	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0002287.2	0.37	NRPS	mutanocyclin, leuvalin, tyrvalin	Streptococcus mutans
BGC0000779.3	0.34	saccharide	lipopolysaccharide	Aeromonas piscicola
BGC0002007.2	0.32	terpene	atolypene A, atolypene B	Amycolatopsis tolypomycina
BGC0001650.4	0.30	NRPS	Le-pyrrolopyrazines	Lysobacter enzymogenes
BGC0002545.3	0.30	PKS	sungeidine C, sungeidine B, sungeidine D, sungeidine H, sungeidine A, sungeidine E, sungeidine F, sungeidine G	Micromonospora sp.
BGC0002387.2	0.29	PKS	yangpumicin A	Micromonospora yangpuensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001209.3	0.62	ribosomal	streptide	Streptococcus thermophilus LMD-9
BGC0001929.2	0.43	ribosomal	WGK	Streptococcus ferus
BGC0000902.4	0.42	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0000285.5	0.37	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0002287.2	0.37	NRPS	mutanocyclin, leuvalin, tyrvalin	Streptococcus mutans
BGC0000779.3	0.34	saccharide	lipopolysaccharide	Aeromonas piscicola
BGC0002007.2	0.32	terpene	atolypene A, atolypene B	Amycolatopsis tolypomycina
BGC0001650.4	0.30	NRPS	Le-pyrrolopyrazines	Lysobacter enzymogenes
BGC0002545.3	0.30	PKS	sungeidine C, sungeidine B, sungeidine D, sungeidine H, sungeidine A, sungeidine E, sungeidine F, sungeidine G	Micromonospora sp.
BGC0002387.2	0.29	PKS	yangpumicin A	Micromonospora yangpuensis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.97 out of 26.06

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.06 out of 19.26

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.21 out of 26.06

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.21 out of 26.06

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.40 out of 19.26

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.93 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.11 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.23 out of 26.06

Confidence: medium

Additional weak hits (2):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.55 out of 32.74

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 24.19 out of 28.14

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
MogR	Repressor of flagellar motility genes