LOCUS       NZ_MTJR01000004        10168 bp    DNA     linear   CON 23-AUG-2024
DEFINITION  Lactococcus lactis strain 417 contig_4, whole genome shotgun
            sequence.
ACCESSION   NZ_MTJR01000004
VERSION     NZ_MTJR01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Lactococcus lactis
  ORGANISM  Lactococcus lactis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Lactococcus.
REFERENCE   1  (bases 1 to 388226)
  AUTHORS   Ho,V., Lo,R., Bansal,N. and Turner,M.S.
  TITLE     Characterization of Lactococcus lactis isolates from herbs, fruits
            and vegetables for use as biopreservatives against Listeria
            monocytogenes in cheese
  JOURNAL   Food Control (2017) In press
REFERENCE   2  (bases 1 to 388226)
  AUTHORS   Lo,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2017) School of Agriculture and Food Sciences, The
            University of Queensland, Hartley Teakle Bldg (No. 83), Slip Rd, St
            Lucia, Brisbane, QLD 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. Jun 2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 793.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002563455.1-RS_2024_08_23
            Annotation Date                   :: 08/23/2024 15:24:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,353
            CDSs (total)                      :: 2,288
            Genes (coding)                    :: 2,273
            CDSs (with protein)               :: 2,273
            Genes (RNA)                       :: 65
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 3 of 15
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:59:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1436
            Orig. end                         :: 11604
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            MTJR01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10168
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10168
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10168
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5168](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5168)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(55..68)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 24.68"
                     /tool="antismash"
     gene            complement(163..996)
                     /locus_tag="BW153_RS00315"
                     /old_locus_tag="BW153_00315"
     CDS             complement(163..996)
                     /NRPS_PKS="Domain: PKS_KR (5-153). E-value: 6.4e-07. Score:
                     21.4. Matches aSDomain:
                     nrpspksdomains_BW153_RS00315_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905089.1"
                     /locus_tag="BW153_RS00315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW153_00315"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_010905089.1"
                     /transl_table=11
                     /translation="MTQKIVAITGASNGMGFEAAELFAKRGWKVYAGARRVEKIPQYEN
                     NIKALKLDVTSSESNQAFVKKILDEAGHIDVLINNAGYGEYGPAEEIPMDKIRNQFETN
                     FFGAVELTQLVLPTMRAQNYGRIVNISSIGGDVYMPLGAYYHATKAALQQWSDVLDLEV
                     AQFGIRSVCVQPGGTQSSWGNIALENSKKNMSSNSAYQPLVNSVASGLDRFMKNSAKAS
                     DLAEVFYKAATEIKPSLRYLHSFGDKFMVNVARKHPKIFRKALLITMRYFEKHNQ"
     aSDomain        complement(538..981)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BW153_RS00315_PKS_KR.1"
                     /evalue="6.40E-07"
                     /label="BW153_RS00315_PKS_KR.1"
                     /locus_tag="BW153_RS00315"
                     /protein_end="153"
                     /protein_start="5"
                     /score="21.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAITGASNGMGFEAAELFAKRGWKVYAGARRVEKIPQYENNIKAL
                     KLDVTSSESNQAFVKKILDEAGHIDVLINNAGYGEYGPAEEIPMDKIRNQFETNFFGAV
                     ELTQLVLPTMRAQNYGRIVNISSIGGDVYMPLGAYYHATKAALQ"
     gene            1397..1864
                     /locus_tag="BW153_RS00320"
                     /old_locus_tag="BW153_00320"
     CDS             1397..1864
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905090.1"
                     /locus_tag="BW153_RS00320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW153_00320"
                     /product="universal stress protein"
                     /protein_id="WP_010905090.1"
                     /transl_table=11
                     /translation="MSKNYKKILVAIDGSEQAEEALKEAIVLAKRDNSQLFVLHATDKN
                     SIYAAGNPVPVVPAPAIPVVPAVPVLEESADNEAKEVLDKALAIINNEVKFEEIRVDGS
                     AKNEIVDFAKEHEIDMIVMGSSGKGALDRMLLGSTAVYVVKHAPCSVTIIK"
     misc_feature    complement(1949..1964)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: strong, score: 30.52"
                     /tool="antismash"
     gene            1992..2189
                     /locus_tag="BW153_RS00325"
                     /old_locus_tag="BW153_00325"
     CDS             1992..2189
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011675138.1"
                     /locus_tag="BW153_RS00325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW153_00325"
                     /product="hypothetical protein"
                     /protein_id="WP_098395973.1"
                     /transl_table=11
                     /translation="MKVTFKTLDGRTMTKEFTSVDEFVTLQNREIPVIDDSAKVLEVVI
                     SGQVEEFSGNVADLYFKLSK"
     gene            2220..2840
                     /locus_tag="BW153_RS00330"
                     /old_locus_tag="BW153_00330"
     CDS             2220..2840
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896940.1"
                     /locus_tag="BW153_RS00330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW153_00330"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_012896940.1"
                     /transl_table=11
                     /translation="MTDYVFKAGRKDLAPLLLLHSTGGDEHQLVEIAEMIAPSHPILSI
                     RGRINEQGVNRYFKLRGLGGFTKENFDLESLDEETDWLTDEVSLLAEKHDLDVHKMIAI
                     GYSNGANVALNMFLRGKINFDKIIAFHGMQLEDFEQTVQLDDKHVFLSYAPNDMIVPQK
                     NFGDLKGDLEDSGCQLEIYESSLGHQLTQEEVLAAKKWLTETK"
     gene            2891..4060
                     /locus_tag="BW153_RS00335"
                     /old_locus_tag="BW153_00335"
     CDS             2891..4060
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425630.1"
                     /locus_tag="BW153_RS00335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW153_00335"
                     /product="ring-cleaving dioxygenase"
                     /protein_id="WP_058221149.1"
                     /transl_table=11
                     /translation="MAELTKIETKIPENLTLGGVHHVTAITSSAQKIYDFFTNILGLRL
                     AKLTVNQDDYETYHLYFTEEDGHGADMTFFDFKDIPKGNHGVNNIERASFRVPTDASLD
                     YWTRRFDNAHVKHGEISSKFGQKTLEFEDFDGQLYQLISDQNNSGDDSKGRSWQLSNVP
                     QEHGITGLGPVFVKVENAENLRTILETVFGFRYAGQEDDLHLFEVANGGNGAALILQEA
                     GEKEAYAYQGYGTIHHLALGTANPETLNYWIERIKAFRLPHSGLVDRFYFSSEYVRVAP
                     GVLFEIATYTPGIGALDMAKSGLGAVDSYEEALITSGFWIDQTKEESGLSLSLPPHLFP
                     GDEATKARTAASLRPLDTSDAHRDRTKDELWTVEKVLEHKAGTPAETVQ"
     gene            4071..4661
                     /locus_tag="BW153_RS00340"
                     /old_locus_tag="BW153_00340"
     CDS             4071..4661
                     /EC_number="1.5.1.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017863958.1"
                     /locus_tag="BW153_RS00340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW153_00340"
                     /product="flavin reductase family protein"
                     /protein_id="WP_003132255.1"
                     /transl_table=11
                     /translation="MKSYQANELDEKTVYKLLSGSIVPRPIAWVTSQNLEGLVNVAPFS
                     FFNVASSNPPLLSISFTGNKDSLNNLLTTKEAVVHLVNEDNVELMNQTAAPLAEHISEA
                     EEFSLELVPSQKVQVPSLKESNVRLETKLYHHLPLGESGHLVLLEVVNFSFAEELLDEE
                     NFHVNLNKLKPVGRLAGDDYSTLGNRFSLLRPR"
     gene            complement(4697..4999)
                     /locus_tag="BW153_RS00345"
                     /old_locus_tag="BW153_00345"
     CDS             complement(4697..4999)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003132256.1"
                     /locus_tag="BW153_RS00345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW153_00345"
                     /product="hypothetical protein"
                     /protein_id="WP_003132256.1"
                     /transl_table=11
                     /translation="MTKYIFPNLKDNQRDLFNIINQILSRDKLSIEERKCFLIAKKNIE
                     SGRNFDPQISELISSLQYLAHSSDVQYFFSEAQKRMLVNPGTGGSLYGWSNFESK"
     gene            complement(5001..5168)
                     /locus_tag="BW153_RS00350"
                     /old_locus_tag="BW153_00350"
     CDS             complement(5001..5168)
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003132257.1"
                     /locus_tag="BW153_RS00350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW153_00350"
                     /product="lactococcin family bacteriocin"
                     /protein_id="WP_003132257.1"
                     /sec_met_domain="Lactococcin (E-value: 4.1e-08, bitscore:
                     27.9, seeds: 3, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENRLNFEAISDDELAKIVGGGYPNNQSMNDVLHWLNGHNDGNPK
                     QLPKWMGGLG"
     misc_feature    complement(5525..5539)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 24.06"
                     /tool="antismash"
     misc_feature    5526..5539
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.95"
                     /tool="antismash"
     gene            complement(5735..5884)
                     /gene="rpmG"
                     /locus_tag="BW153_RS00355"
                     /old_locus_tag="BW153_00355"
     CDS             complement(5735..5884)
                     /GO_component="GO:0005840 - ribosome [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpmG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /locus_tag="BW153_RS00355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW153_00355"
                     /product="50S ribosomal protein L33"
                     /protein_id="WP_003132258.1"
                     /transl_table=11
                     /translation="MRVNITLEHKESGERLYLTQKNKRNTPDKLELKKYSKKLRKHVIF
                     KEVK"
     gene            complement(5914..6087)
                     /gene="rpmF"
                     /locus_tag="BW153_RS00360"
                     /old_locus_tag="BW153_00360"
     CDS             complement(5914..6087)
                     /GO_component="GO:0015934 - large ribosomal subunit
                     [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpmF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905107.1"
                     /locus_tag="BW153_RS00360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW153_00360"
                     /product="50S ribosomal protein L32"
                     /protein_id="WP_010905107.1"
                     /transl_table=11
                     /translation="MAVPARHTSSAKKNRRRTHYKLTAPTVTFDETTGDYRHSHRVSLK
                     GYYKGRKVRDTK"
     gene            6451..8232
                     /locus_tag="BW153_RS00365"
                     /old_locus_tag="BW153_00365"
     CDS             6451..8232
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1037: heavy metal
                     translocating P-type ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017863960.1"
                     /locus_tag="BW153_RS00365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW153_00365"
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="WP_021722893.1"
                     /transl_table=11
                     /translation="MKNWKKLILVFVIAGVALIAYFGFNQPFLARLIVTIAGGVLAFSM
                     LIEMIKTLRSGSYGVDLLAITAIIATLLVGEYWASLIIILMLVGGESLEDYANGRAHRA
                     LAALLDKSPDIAHVQKGDKVVDLPIDHVEVGSHLLIRPMEIIPIDGKLISEAVVLDTAS
                     LTGETKPNELVKGDPVLSGAINGNSSIQIETTVIAAESQFQKIVELVKETEKTPAQFVR
                     LADRYAVPFTIIAYLIAAVAYFISGDPVRIAEVLVVASPCPLILAAPIAFVSGMSRSSQ
                     NGALIKNGTVIEKLAKAREIFLDKTGTITEGTIKVDSLKPIETVDEVELLQLVYTVEKS
                     SGHILAKAVVEYAENKKVQPLKLTSLTEVAGLGVIGEIEGQEIKIGRSSFAFAPDNLNY
                     ETAFYVSKAGKYVGAVTFSDTLRPEAKETIAQMRKIGLEKITMLTGDNQLVADKIASTV
                     GIDNVYAGLMPDEKLKFIKNATNRPVIMVGDGVNDAPALATADVGISIGVGSGTVASEA
                     ADIVLLQNDLRKVTTSIEISRDTLKIAKQAVMIGIIICIILMFIAALGVIPAVIGALFQ
                     EIIDVVSILYALRALRG"
     gene            complement(8282..9067)
                     /locus_tag="BW153_RS00370"
                     /old_locus_tag="BW153_00370"
     CDS             complement(8282..9067)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014571835.1"
                     /locus_tag="BW153_RS00370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BW153_00370"
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="WP_021722894.1"
                     /transl_table=11
                     /translation="MVEQITQLKLSTIVKNPYQPRLVFDEDKLKELANSIQENGVLQPI
                     IVRKSKLVGYELLAGERRFQASKLAGLETIPAIIRSYSDEEMMTLSILENLQRENLNPL
                     EESKSLAQLADKLGMTHDQIAKALGKSRSYVSNLIRLLGLPDTILKHVENREISPAHAR
                     TLLAEKNPKKQLELVDKIIKEQLNVRALEAIIYGEEKVTDKSVSKLNINLFTDETEKEL
                     MKKLGNRVKIQANKKYQGNLSIHFDNLDELEHLIKLLKK"
     misc_feature    complement(9238..9256)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.1"
                     /tool="antismash"
ORIGIN
        1 ttgattattc gtcagcaatt cttgaaccat agctttctcc tttagctata aaacttatta
       61 taattataag aaattcagaa aattctagcg atatgatttt ttaatggagc tgatagaact
      121 tttaaaagta gaaataaaaa aatacaactc aaggttgtat ttttattgat tgtgcttttc
      181 aaagtaacgc attgtaataa gaagagcttt acgaaagatt tttggatgtt tacgagcaac
      241 atttaccatg aatttatcac cgaaagagtg gaggtagcga aggctcggtt taatctccgt
      301 cgcagcttta taaaaaactt cagctaaatc agaagcttta gcactatttt tcataaaacg
      361 gtcaagtcct gaagcaacag aatttaccaa aggttggtaa gcagagtttg aactcatatt
      421 ctttttagaa ttttctaggg caatatttcc ccagcttgac tgagttccac caggttgaac
      481 acaaactgaa cggataccaa attgggcaac ttcgaggtcc aaaacgtcag accattgttg
      541 aagggcggct tttgtagcat gataataagc accaagtggc atatagacat ctccaccaat
      601 tgatgaaata ttaacaattc gtccataatt ttgagctctc attgttggta agacaagttg
      661 agttaattca accgcaccaa agaagttggt ttcaaactga ttgcgaattt tatccattgg
      721 gatttcttcg gctggtccgt attcgccata acctgcatta ttgatgagga cgtcaatatg
      781 acctgcctca tctaaaattt ttttcacaaa agcttggttt gattcagagc tagtaacatc
      841 cagttttagt gctttgatgt tgttctcgta ttgtggaatt ttttcaacgc ggcgtgcccc
      901 agcgtatact ttccaacccc gtttggcaaa aagttctgct gcctcaaatc ccattccatt
      961 tgaagcacct gtgattgcaa caattttttg tgtcatctct ttagagtcct ttcaatttgt
     1021 ctgtatcttg ttttcatgat tttaaaagta attataccac tttagtaaat caaaggctaa
     1081 aaaaacttcc gcggagtaaa cggaagtttt aggagtattt atgaaaaaag tttatttagg
     1141 agtatggaaa ctattatacg ttataatgct gaaatgaaac ttaaatgaaa aagaaaatgt
     1201 ttttgtattt ttagaatccc ttttctataa atcaattcta actataagga cctgaagatt
     1261 gagtgataat actagtttga agcattctta gtaagaaagt cattttttat aaatggttta
     1321 tagaataaat tgtacagcgt ttaattggac ttgctctctg aaataacgta aaattgtagt
     1381 gaggaggacg gttacaatga gtaaaaatta taaaaaaatt ctagtcgcaa ttgatggatc
     1441 tgagcaggca gaagaagcgt taaaagaagc tattgtcctt gctaaaagag acaactcaca
     1501 attattcgtt ttacatgcga cggataaaaa cagtatttat gcagctggta acccagttcc
     1561 tgtggttcct gcaccagcta ttccagtagt tccagcagtt cccgtacttg aagaaagtgc
     1621 agataatgaa gctaaagaag ttttagacaa agctttggca attatcaata atgaagtgaa
     1681 gtttgaagaa attcgagttg atggttcggc aaaaaatgaa attgtcgatt tcgctaaaga
     1741 acatgaaatt gatatgattg tgatgggctc ttcaggaaaa ggcgctcttg accgaatgtt
     1801 gcttggctct acagcagttt acgtggttaa acatgcacct tgtagtgtaa caatcatcaa
     1861 ataaattaga tagatttcaa gaatcggcaa gcatttgctg atttttttgt attttaaaaa
     1921 taaattcctt gacaatgaaa ttcttttcgt ttacaattgt aaacatagaa atgagaaagt
     1981 tggattgaac aatgaaagtt acttttaaaa cccttgatgg acgtacaatg actaaagaat
     2041 ttactagtgt agatgaattc gtaacactac aaaatcgtga aattccggta attgatgatt
     2101 cagcgaaagt tcttgaagtt gtaatttctg gacaagttga agaatttagt ggaaatgttg
     2161 ctgatttgta tttcaaactt agtaaataag aatctgaaat cgtgaatgga ggaggaaaga
     2221 tgacagatta tgtttttaag gcaggtcgga aagatttggc acctcttttg ttattacata
     2281 gcacgggtgg agatgagcat caattagtag aaattgctga aatgattgcc ccttcacatc
     2341 ctattttgtc aattcgtgga cgtattaatg agcaaggagt caatcgttat tttaaactcc
     2401 gaggcttagg tggttttact aaagaaaatt ttgatcttga atctttagat gaagaaacag
     2461 actggttgac ggatgaggtc agtttgttgg ctgaaaaaca tgaccttgac gtccataaaa
     2521 tgatagctat cggttattca aatggtgcaa atgtagcttt gaatatgttt cttagaggaa
     2581 aaataaattt tgataaaatt attgcctttc atgggatgca attggaagac tttgaacaga
     2641 ccgttcaact tgatgataag catgtctttc taagctatgc gccaaatgat atgattgttc
     2701 ctcagaaaaa ctttggtgat ttaaaaggtg atttggaaga ttcaggctgt cagcttgaaa
     2761 tatatgaaag ttcacttggt catcaattga cgcaagagga agttttagcc gctaaaaaat
     2821 ggcttacaga aacaaaataa ttttgtattg aaaaaatttc agtgcaaagt aactaaatag
     2881 gagaaaacaa atggctgaat taacaaaaat tgaaacaaaa atacctgaaa atcttactct
     2941 tggtggtgtt catcacgtaa cagcaatcac ctcaagtgct caaaaaatat atgatttttt
     3001 cacaaacatt ttgggacttc gcttggcaaa attaaccgtt aatcaagatg actatgaaac
     3061 ttatcacctc tatttcacag aagaagatgg gcatggagct gatatgacat tctttgattt
     3121 caaagatatt cctaaaggaa atcatggggt taataatatc gaacgtgctt cattccgtgt
     3181 accgacagat gcatccttag attattggac ccgtcgtttt gacaatgccc atgttaaaca
     3241 tggcgaaatt tctagtaaat ttggtcaaaa aaccttagag ttcgaagatt ttgatggtca
     3301 attatatcaa ttgatttctg accaaaataa tagtggggat gattcaaaag gacgttcttg
     3361 gcaactttca aatgttccac aagagcatgg aattactgga cttggcccag tttttgtaaa
     3421 agttgaaaat gctgaaaatc ttcggaccat tttggaaaca gtttttggct tccgttacgc
     3481 tggtcaagaa gatgatttac atctttttga agtggcgaat ggtggaaatg gagcagcttt
     3541 gattcttcaa gaagctggtg aaaaagaagc ttacgcttat caaggttatg gtacgattca
     3601 tcatttagct ctgggaacag caaatcctga aactttaaac tattggattg aacgcattaa
     3661 ggctttccgt ttgccacatt ctggtttggt tgaccgtttc tatttctcaa gtgaatatgt
     3721 cagagtagca ccaggagttc tctttgaaat tgcgacttac acaccaggga ttggggcatt
     3781 ggacatggca aaatcaggtc taggagctgt tgattcttat gaagaagctt taattacttc
     3841 tggtttctgg attgaccaaa ctaaggaaga atcaggttta tctttgagct tacctcctca
     3901 tttattcccc ggagatgaag ctactaaagc gagaacagcc gcaagtttac gtccattgga
     3961 cacttcagat gctcatcgtg accgcacaaa agatgaatta tggactgttg aaaaagtatt
     4021 ggaacacaaa gctggaactc cggctgaaac agtgcaataa aggagaactg atgaaatcat
     4081 atcaagcaaa tgagctcgac gaaaaaacag tctataaact tctgtcaggc agtattgttc
     4141 cacgcccaat tgcttgggtg accagtcaaa atctcgaagg tctagttaat gtggcaccct
     4201 ttagtttttt caatgttgct tcgtcaaatc caccactttt atccatttcg tttacgggaa
     4261 ataaagatag tttgaataat ttattgacga cgaaagaagc agtggttcac cttgtgaatg
     4321 aggataatgt ggagcttatg aatcaaacgg cagcaccatt agcggagcat attagtgaag
     4381 cggaagaatt ttcacttgaa cttgtccctt ctcagaaagt tcaggttcca agtttaaagg
     4441 agtcaaatgt tcgtttagaa actaaattgt atcatcattt acctttagga gagagtgggc
     4501 atttagttct tcttgaagtt gtcaatttct cttttgcaga ggaactattg gatgaggaaa
     4561 actttcatgt caatctaaat aaactcaaac cagttgggcg tttagcgggt gatgattaca
     4621 gtaccttagg aaatcgattc agccttttac gacctagata aataaaaaaa cttactgatg
     4681 tttggtcagt aagtttttat ttgctttcaa agtttgacca tccatacaga gaaccaccag
     4741 tacctggatt taccaacatt cttttctgtg cctctgaaaa aaaatactgc acatcagatg
     4801 aatgagcaag atactgcaaa ctagaaatca attcactaat ttgaggatca aaatttcgac
     4861 cactttcaat atttttttta gctatcaaaa aacattttct ttcttcaatg gaaagtttat
     4921 ctctagataa aatttgatta ataatgttga ataaatctct ctggttatct tttaaatttg
     4981 gaaaaatata ttttgtcatt ttaacctaaa ccacccatcc atttaggtaa ttgtttggga
     5041 tttccatcat tatgaccatt gagccaatga agaacatcat tcatgctttg gttattaggg
     5101 tatcctcctc caacaatttt ggcaagttcg tcatcagaaa tagcctcaaa atttaatcga
     5161 ttttccataa taatctcctt ttcttaatat cactatttta atactattcg ttccatcttt
     5221 cattagcata cgttacacat caatgttgtc ttaatattat tctattttta taattaatca
     5281 agcctaccta tctatactat aaggtaagag taaatcacta ttttgataga tatatattct
     5341 gataagggtt catttttgaa ggggatgctt gttgtaacta attttttatt ctcatgtcta
     5401 aaatttttca caacactatt ttcttcatta aaaaaacaca atagtggaaa aataggagaa
     5461 atggagcgag tggttaaaat cagctcataa cttaacagtt atggctattt tatatctagt
     5521 tttgaatttt ttgaaaaaag ttaagagaag gtaaaatgaa gaaaaaatcc acttccttat
     5581 gtggaggtgg atttgattac ccaaatataa atttctcaaa ggtagagaaa aaggtagaga
     5641 ttcaattgat ttttattgaa atgtaatgaa agtcaagttt tttcaaacac ctctaaatca
     5701 atattttaca agtctattga ctcgtaacga atccttattt aacttcttta aagattacgt
     5761 gtttacgcaa ttttttagag tattttttaa gttccaattt gtctggagtg ttgcgtttgt
     5821 tcttttgagt aaggtacaga cgttcaccag attctttgtg ttcaagtgta atatttacgc
     5881 gcatgatagt ccctttctat aacttttgtc gaattattta gtgtcgcgaa ctttgcggcc
     5941 tttgtagtag cctttaagtg aaacgcgatg tgagtgacgg tagtcaccag tagtttcgtc
     6001 aaaagtaaca gttggagctg tcaatttgta atgtgtacga cgacggtttt tctttgctga
     6061 tgaagtgtga cgtgcaggta ctgccattgt ttttttcctc cgtagaattt tatctcgttc
     6121 ttgcagagtg caaaaacgta ctttattagt atagcataaa ttttcagaaa tgcaagtttt
     6181 gtaaagtaaa attacttgac aaatttttgc aaacctttct gtcagtaaaa tttgagtaag
     6241 caaaagtact gacaaatatc tttgacaact aaaatgatag ggcctttagc gcaaatttga
     6301 taaaatagaa gtaacttaac ggataactta aaaatttaag ctcagcttcg aaagaatcaa
     6361 agcggcgtaa ggagaagaca atcaggtcgt aaaaactcct cacaaagcag ggcaatgttc
     6421 taggacaatt aaatttttaa ggagaaatga atgaagaact ggaaaaaact gattcttgtt
     6481 tttgtcattg caggcgtggc attgattgct tactttggct ttaatcagcc ttttttggca
     6541 cgcttaattg ttacaattgc tgggggagtc ttggccttct caatgctgat tgaaatgatt
     6601 aaaactttac ggtctggctc ttatggtgtt gacttattgg ccatcacggc cattattgct
     6661 actttactag tgggtgaata ttgggcctct ttgattatta ttctcatgct tgttggaggt
     6721 gagagtttag aagactatgc aaatggtcga gctcaccgag ctttagctgc tttattagat
     6781 aagtcgcctg atattgctca tgtgcaaaaa ggagataagg tggtcgattt accgatagac
     6841 catgtggaag tcgggagtca tttattgatt agaccgatgg aaattattcc aattgacggg
     6901 aaacttattt ctgaggcagt ggtacttgat acagcgtcat tgacgggtga aaccaagccg
     6961 aatgaattag taaaaggtga tcctgttctt tcgggagcta tcaatgggaa ttctagtatc
     7021 cagattgaaa ctacggtgat agcggcagaa tcacagtttc aaaagattgt agaactggtt
     7081 aaagaaacgg agaaaactcc agctcagttt gtacgtctgg ctgatcgtta tgctgttcct
     7141 tttacaatta ttgcctatct tattgcagca gttgcttact ttatttctgg tgacccagtg
     7201 cggattgcgg aagtcctagt ggttgcaagc ccttgtccat tgattctggc agcccctatc
     7261 gcgtttgttt cgggaatgag tcgctctagt caaaatggag cattaatcaa aaatggaaca
     7321 gtcatcgaaa aattagcaaa agctcgggaa atatttttag ataaaacagg tacaattact
     7381 gaggggacaa ttaaggttga cagtctaaaa ccaatagaaa cggtagatga agtagaactt
     7441 ttacagttgg tttacacggt tgaaaaatca agtggtcata ttttagcaaa agctgttgtt
     7501 gaatatgctg aaaataaaaa ggttcaacca ttaaaattga cctctttaac tgaggttgct
     7561 ggacttggtg tgattggaga aatcgaaggt caagaaatta aaattgggcg aagttctttt
     7621 gcttttgcac ctgataatct caactatgaa acagcatttt atgtgagtaa agcaggtaaa
     7681 tatgttgggg cggtcacatt ctctgatact ttaagaccag aagctaaaga aacaattgct
     7741 cagatgagaa aaattggtct tgaaaagatt acaatgttga cgggtgacaa tcaacttgtt
     7801 gctgataaaa ttgcgagcac tgttggaatt gacaatgttt atgcaggttt gatgccagat
     7861 gaaaaattaa aatttattaa aaatgcgacg aatagaccag taattatggt tggagatgga
     7921 gttaatgatg ctccagctct agccacagca gatgttggaa tttcgattgg tgtgggctca
     7981 ggtacagttg caagtgaagc ggctgacatt gttctattgc aaaatgacct taggaaagtg
     8041 acgacatcta ttgaaatcag tcgtgatacg ttaaaaattg ctaaacaagc ggtcatgatt
     8101 gggattatca tttgtattat tttgatgttt attgcagctc tgggagtgat tccagctgtg
     8161 attggagctt tgttccaaga gatcattgat gttgtttcaa ttctctatgc gcttcgggct
     8221 ttaagaggat aaaaaaattt actgacggaa tgcttaaaag tcctctgtca gtaaattttt
     8281 tttatttttt tagtaatttt attagatgtt ccagttcatc aagattatca aaatgaatgg
     8341 ataaattccc ttgatatttt ttatttgctt gaatcttaac cctatttccc aattttttca
     8401 tcagttcttt ttcagtttcg tcagtaaaaa gattgatatt taatttactg acagatttgt
     8461 cagtaacttt ttcttcacca taaataatag cttcaagtgc tctgacattg agctgctctt
     8521 taattatttt atcaacgagc tctaattgct ttttaggatt tttttcagct aaaagtgttc
     8581 tagcgtgagc aggtgaaatc tctcgatttt caacatgctt caaaattgtg tcaggcaaac
     8641 caagtaaacg aataagatta gagacataag accttgattt tccaagggcc ttagcgattt
     8701 gatcgtgggt cattcccaat ttgtcagcca gttgtgccaa gcttttggac tcttctagtg
     8761 gattgagatt ttctctctgc aaattttcta aaattgagag agtcatcatt tcttcatcag
     8821 aatagcttct aataattgct ggtatagttt ctaaaccagc taattttgaa gcttgaaacc
     8881 gacgttcacc tgctaaaagc tcatagccaa caagttttga tttacgaacg attatgggtt
     8941 gtaaaacgcc attttcttgg atagaattag ccaattcttt taatttatcc tcatcaaaaa
     9001 caagacgagg ctggtaaggg ttttttacaa ttgttgacaa cttaagttga gtgatttgtt
     9061 ctaccatatt tttcactttc tgtcagtaaa ttttattgtg ctctatttac tgacagattt
     9121 gtcagctcct ctttttttgt tcatcaatcc attctttacg actgtggagt gctttttcat
     9181 atcttcccat atcatcagga cggaaataat cagcattttt aattttatca ggtaagtact
     9241 gttggtttac ccagtgatta ggaaaattat gagcatattg gtattctaca gaccttccta
     9301 aatcctttgc cccagcgtaa tggccatcct gtaagtgtgg tggtacagca agatttccat
     9361 attttcctaa atcagcaatg gcttcatcca tcgctaaata agccgcattt gatttagggg
     9421 ataaagccaa gtcaataatg atattggcaa ggggaatccg tgcctcagga aaaccgagtt
     9481 tttcggctgc tgataatgct gaaaccgtat gaagtgcggc atcaggattt gctaaaccaa
     9541 tatcttcata agccataacg gttaagcgcc gggctagact ctgcaaatct ccgccctcaa
     9601 ttaaacgagc cgcatagtgt aaacttgcgt tgacatcaga accacgaatt gacttttgta
     9661 aagcagaaag caagtcataa tgagcatcgc catctttatc aaaagttgca gcttttcttt
     9721 gcaaagaatt ttccatatca tctaaagtga catgatgttc ttctgaggaa agaacggcta
     9781 attctaaggc attaaaagta gaacgtaaat ccccattagt cgaatgaatt aaaaagtaaa
     9841 gtgcatcatc gtcaatagtc acatcaaaat caaatccacg ttctttatct tgtaaactta
     9901 ggtctacagc aaattctaaa tcttctggtt ctaaaggttt taattcaaaa atctgtacgc
     9961 gagaacgaat cgctggaaca actgaaaaat aagggttttc ggttgttgct ccaattaaaa
    10021 taatttgtcc attttctaaa agtggtaaaa gaaaatcttg tttaggtttg tctaggcgat
    10081 gaatttcatc aagtaataaa acaagctgac cagaaaattc ggcttctgag gcaatttctt
    10141 gaagtctttt tttagtgtct gtcgttgc
//
