LOCUS       NZ_NTSF01000032         7284 bp    DNA     linear   CON 02-APR-2024
DEFINITION  Bacillus thuringiensis strain AFS021634
            AFS021634_110_H4_Contig32_140523, whole genome shotgun sequence.
ACCESSION   NZ_NTSF01000032
VERSION     NZ_NTSF01000032.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 65953)
  AUTHORS   Bleich,R.M., Kirk,G.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 65953)
  AUTHORS   Bleich,R.M., Kirk,G.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Assembly Cell v. 4.2.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 48.88x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002563165.1-RS_2024_04_02
            Annotation Date                   :: 04/02/2024 02:22:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,903
            CDSs (total)                      :: 5,824
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 84 of 212
            Pseudo Genes (incomplete)         :: 133 of 212
            Pseudo Genes (internal stop)      :: 67 of 212
            Pseudo Genes (multiple problems)  :: 60 of 212
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:49:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 7284
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NTSF01000032.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..7284
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..7284
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..7284
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1954:2284](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(1955..2284)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(<1..665)
                     /locus_tag="CN325_RS24210"
                     /old_locus_tag="CN325_24210"
     CDS             complement(<1..665)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736205.1"
                     /locus_tag="CN325_RS24210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_24210"
                     /product="DUF3962 domain-containing protein"
                     /protein_id="WP_141534893.1"
                     /transl_table=11
                     /translation="MKKLRLLTFENIVDPLLNEKVSFIYFPIEWLDIVEIHYKTFLLTS
                     KLKRLNERLYDMFSDILFIQHNPYVLNENTPWIVSKEPIKQEQLDYIFQSWYEIIHDWK
                     PNRLVEPPKYEWQSDLISNLPILHDNETYSKWVPALISHIFCERPIYLENTNAEEIYFS
                     PLRSQNICEAMSGPIKDEKTQDFFSYVYRFECITRGGENAPLLNISIGIRRFYQEYKM"
     misc_feature    complement(657..670)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.3"
                     /tool="antismash"
     gene            complement(976..1461)
                     /locus_tag="CN325_RS24215"
                     /old_locus_tag="CN325_24215"
     CDS             complement(976..1461)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124614.1"
                     /locus_tag="CN325_RS24215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_24215"
                     /product="hypothetical protein"
                     /protein_id="WP_002041181.1"
                     /transl_table=11
                     /translation="MNMIELKSIIHSYKLKRKIAKDLYGKRDELTLLLNEFNKMKCTVT
                     SEKKKNNILSRLQLIYQNMKLDKQYPLSVAFDSKLLERLEKEPLHTIEDGVTCLHLMLD
                     VNYEKIKQYGSSTSRSFVPLSQSSICLADCICLTGVVVGLLGAISFGGLLLSICSIT"
     misc_feature    1493..1511
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.87"
                     /tool="antismash"
     misc_feature    complement(1562..1579)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.24"
                     /tool="antismash"
     gene            complement(1605..>1805)
                     /locus_tag="CN325_RS30705"
                     /pseudo=""
     CDS             complement(1605..>1805)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012132.1"
                     /locus_tag="CN325_RS30705"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="YMYVPTKNIAIINGYIQSFK"
     gene            complement(1955..2284)
                     /locus_tag="CN325_RS24220"
                     /old_locus_tag="CN325_24220"
     CDS             complement(1955..2284)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071363.1"
                     /locus_tag="CN325_RS24220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_24220"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_141534894.1"
                     /sec_met_domain="TIGR03601 (E-value: 2.7e-40, bitscore:
                     128.8, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQALSLNDYRSGNIVYWDQQNQYPYYYIEDATRRCGGC
                     GRCGGCGGGRCGGFRCGGFRCIGCFGCFGCGGCGGCGGCGGCSNCFDGFSGTTDTTGTI
                     ITYEY"
     gene            complement(2817..3080)
                     /locus_tag="CN325_RS24225"
                     /old_locus_tag="CN325_24225"
     CDS             complement(2817..3080)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101034.1"
                     /locus_tag="CN325_RS24225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_24225"
                     /product="DUF3937 domain-containing protein"
                     /protein_id="WP_002082716.1"
                     /transl_table=11
                     /translation="MNFERCYMFTNKKLIRFGLSLFVFLGIINFTISYFQTYLETAADI
                     KWVIPEIWKTILLDVPQGILVLLGAIALYDFTKEASQKDASI"
     gene            complement(3478..4167)
                     /gene="tenA"
                     /locus_tag="CN325_RS24235"
                     /old_locus_tag="CN325_24235"
     CDS             complement(3478..4167)
                     /EC_number="3.5.99.2"
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="CN325_RS24235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_24235"
                     /product="thiaminase II"
                     /protein_id="WP_098015609.1"
                     /transl_table=11
                     /translation="MTFSQSLRKEVDSIWEASFNHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITKEEKDNFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWISAY
                     GSDWFRTLVEEQITRLDTIAEKVTVADRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDT
                     DVKDVIG"
     regulatory      complement(4295..4400)
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     misc_feature    4439..4451
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     gene            complement(4474..5388)
                     /locus_tag="CN325_RS24240"
                     /old_locus_tag="CN325_24240"
     CDS             complement(4474..5388)
                     /EC_number="6.3.2.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="CN325_RS24240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_24240"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_003270605.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 1e-23, bitscore:
                     76.0, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 1.5e-15, bitscore:
                     49.7, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 1.5e-10, bitscore: 33.0,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 5.4e-55,
                     bitscore: 178.1, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 1.5e-07, bitscore:
                     23.2, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRY
                     EGVETPDWIELTKMEDLNLDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVF
                     EWDSEVVIEKYIKGDEITCSILDGKQLPIVSIRHAAEFFDYNAKYDDTSTVEEVIELPA
                     EINERVNKASLACYKALKCSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAG
                     ISYSKLLDMIIETSLKVRNEEGF"
     gene            complement(5393..6841)
                     /locus_tag="CN325_RS24245"
                     /old_locus_tag="CN325_24245"
     CDS             complement(5393..6841)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-469). E-value:
                     5.7e-30. Score: 96.6. Matches aSDomain:
                     nrpspksdomains_CN325_RS24245_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="CN325_RS24245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_24245"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_000471611.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 6.7e-30, bitscore:
                     96.5, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYLKKMIMKGHLLGDQKIPSTRELSEL
                     LSVSRNTVLAAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKNKLNTVTALADE
                     LDLMKHGVRWEKGMIVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYRPL
                     MNYLLHYMEMKGVDISNKDILITNGFTEGLDIVLSSLSKKSGRVICENPTHHAALKLFR
                     LHGLEVHGIDMHEDGIDTNEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYNVPIIEDGFNEELRYSGSHLAPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQY
                     IPFKRMTGDGGLHLFIELEEHMNARTLLQKCYEKGVTFSPGDVFYSDGEGANTFRLGFS
                     RLKEEEIVRGIKIIGDTLKNENWS"
     aSDomain        complement(5435..6406)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CN325_RS24245_Aminotran_1_2.1"
                     /evalue="5.70E-30"
                     /label="CN325_RS24245_Aminotran_1_2.1"
                     /locus_tag="CN325_RS24245"
                     /protein_end="469"
                     /protein_start="145"
                     /score="96.6"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYRPLMNYLLHYMEMKGVDISNKDILITNGFT
                     EGLDIVLSSLSKKSGRVICENPTHHAALKLFRLHGLEVHGIDMHEDGIDTNEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYNVPIIEDGFNEELRYSGSHLAPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQYIPFKRMTGDGGLHLFIELEEHMNARTL
                     LQKCYEKGVTFSPGDVFYSDGEGANTFRLGFSRLKEEEIVRGI"
     misc_feature    complement(6917..6931)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 17.35"
                     /tool="antismash"
ORIGIN
        1 atcatcttat attcttgata aaatctccga atccctattg aaatatttaa taatggagcg
       61 ttctcaccac cgcgggttat gcattcgaat cgatatacat aggagaaaaa atcttgtgtt
      121 ttttcatctt ttatcggccc tgacatcgcc tcacaaatat tttgtgatct aagaggggaa
      181 aaatagattt cttctgcatt tgtattttct aaatatatag gacgctcaca aaaaatatgt
      241 gagattaaag cgggcaccca cttagaatac gtttcattat catgtagtat tggcaaatta
      301 gaaatcaaat cggattgcca ttcatatttt ggtggttcta ctaatctatt tggtttccaa
      361 tcatgaataa tttcatacca actttgaaaa atataatcga gctgctcttg tttaataggt
      421 tcttttgata caatccatgg tgtattttca tttaatacgt acggattatg ctgaataaac
      481 aatatatccg aaaacatatc atacaatctt tcattcaaac gtttcaactt actcgttaat
      541 aaaaatgtct tataatgtat ctctacgata tccagccatt caataggaaa gtatataaat
      601 gatacctttt catttaaaag ggggtctact atattttcaa atgttagcag ccttaatttt
      661 ttcataaaat gattccttcc tttcttcagt tgtcttgatt atcaaaaagc tagaacaata
      721 gttacttaat ctaatacatt taaacaaagt atttaaaaga aacgtaaaaa ctgcttatct
      781 acataaatca ttatcaaacc gactctttta aaaattaaaa gtaattatca tatacaacac
      841 ctctttatat tcgtgtttta atacatctga attatacaat gtatataata tattgtactt
      901 atcatttaca ataactttac aaaaaaacca aaaaagaaca ccttaattcg gtgccctttt
      961 tacaaatatc aacctttatg taattgaaca tatagataac agtaaacccc caaatgaaat
     1021 tgctcctagt aaacctacta caactcctgt taaacaaata caatcagcaa gacaaataga
     1081 agactgcgat aatggaacaa atgacctact tgtactcgaa ccatattgtt ttattttctc
     1141 ataattcaca tctaacatta gatgtaaaca tgttacacca tcctcaatag tatgtagagg
     1201 ttctttttct aatcgctcca ataatttact atcaaaagca actgatagag gatactgctt
     1261 atctaatttc atattttgat aaattagttg caaacgagat aatatattgt ttttcttctt
     1321 ttcagatgtt actgtacatt tcattttatt aaattcattt aataacagtg tcaattcgtc
     1381 tctttttccg tataaatctt tagctatttt tctctttagt ttatacgaat ggataataga
     1441 ttttagttca atcatattca tacacttcct aattacatat tatttacccg attattttat
     1501 agataaacag tactttttaa tcttatttaa attacactca agcatcttca tttactatcg
     1561 attaacatta cattaacctt acaaagttgt aataaattca ttagctagaa gaaatcaatc
     1621 cctaaaccag acagatattt gatgatacaa ccataagaaa aaagcaatga gtagatttta
     1681 aatctagtca ttgctttatc catcacgtct tgattacatc tatatatctt attgttactc
     1741 tttcacttga atgattgaat atatccatta ataatggcta tattctttgt cggcacgtac
     1801 atatataaaa ctaatgtttt ttaattgata aagtgaagct ttaattagcc ctcaccaatc
     1861 gggcttttat gggtagtccg ccacctaact tctctttgct ctcgctgaat ttttttaggg
     1921 tcttactgcc cggcaaatag caggataaat gcaatcaata ttcatacgtt ataatagtac
     1981 cagtagtatc agtagtacca ctaaaaccat caaaacagtt agagcaacca ccacagccgc
     2041 cacaacctcc gcaacctccg caaccaaaac aaccgaagca accaatacaa cggaatccac
     2101 cacaacggaa tccgccacaa cgacctccac cacaaccacc acaacgacca caaccgccac
     2161 agcgacgagt agcatcttca atatagtaat atggatattg attttgttgg tcccaataga
     2221 caatatttcc agatcggtaa tcattaagac ttaacgcttg tagttcttgt tgaaactgat
     2281 tcattttcat aacctccgtt tataaaatac aacctcatcg atgcttccct atatttctgt
     2341 tcgttacatc taagtaaaaa tagcgctata aactaagttc aatacgtaca ccaacaaagt
     2401 atgacttatc actaaatgat gcaccttgtt catataccta tttttgctat gggctcattt
     2461 ttataaagtg aaactttaat cagtgggggg agttcatccc ccgctgatta ttaaccctca
     2521 ccaatcnncg attggtgagg gttaataaaa attaaaaaat atgaattcta gcgtcatatc
     2581 ttcataccta ctaaattcaa ttaacttctc aataattatg gtgccatatc ctatatattt
     2641 ccgtctgcct cttagatttt tctgtaatgt aaatgcgttt cgctataaca tctcctagtg
     2701 accggtttct atgacgaata agaaattcct acaaagaaaa aagcccttat tagggctttt
     2761 cattctattt ctccagcaaa actctctatg aaattcctaa tagaaaaaag acacacttag
     2821 attgatgcgt ctttttgtga tgcctctttt gtgaaatcat ataaagcaat tgctcctaaa
     2881 agaacaagta taccttgagg aacatctagc aaaatagttt tccaaatttc tggaattacc
     2941 catttaatat ctgctgctgt ttcaagatat gtttggaaat agctgattgt aaaattaatt
     3001 atccctaaaa atacaaacaa cgataaacca aatcgaatta atttcttatt tgtaaacata
     3061 taacacctct caaaattcat tggatattcc cgcttttgct tctttatatt tccatatctc
     3121 tatatctaat tttaacacat aaagagctca aaaaaatacg atatgcatat ttgcatatcg
     3181 tattaacaac actccatgat aatttagaaa ttcctgcaaa gaaaaaacaa ctccgttaca
     3241 gctaacagct cgggtttctt ttcttattta acttcatacc accaacattt acgttctttg
     3301 attgtaaaat aaatgtgaca gaagtttatc cagttaatgc gtagacaagt aaatgagcta
     3361 aattatcgat attctatttc gccatatccg tagcactaat gactttacgc aaatttgaca
     3421 agaaaaaata tatagatagg tatgatccat acctatctat aacattactt ctattcttta
     3481 tccaattaca tcttttacgt ctgtatccac tggccacttt tctaatgtat aagccatttc
     3541 ccaaaatgaa tattcatatt gactgctaat aataaaatgc tgtttcatac gtttgcggtc
     3601 tgccactgtt actttttcag cgatagtatc taaccgtgta atttgctctt caactagcgt
     3661 acggaaccaa tcagatccat aagcagaaat ccattcgtta taaattggct cttccggctg
     3721 gcactctttt aagcgttcgc caatttcata atacaaccaa tagcaaggta aaattgctgc
     3781 aatgatatct cctaaatgcc cttcatacgc agcacgatac atatgtgaag tatacgcata
     3841 tgcagttggc gcaggaataa aattatcttt ttcttctttt gttatcccta gctttttagc
     3901 aaaattctca tgtaaagata actcggcctc atatgtattt tgagcatgat gtgccatacg
     3961 agctgtcgtc tctaattcaa gagcttttgc agctcctaaa gtttgtactc tagcaaaatg
     4021 actcaaataa tatgaatctt gaagcacata atagcgaaaa ctagctaaat ctaatgttcc
     4081 ttcaccaagt ttttttacaa aaggatggtt aaaactcgct tcccaaattg aatctacttc
     4141 tttacgtaat gattgtgaaa aagtcattta aatccctcca ataatgtata aaatataagt
     4201 atttatccac aattattaaa gctgtaaaat aaaaaaactt tctttacagg cgtaaagaaa
     4261 gtgaaattga cagtatttat ttactcatta aatagcaacg tctttcccac ttccctacgc
     4321 tggcattaac cagatcaggt actaagagtc tcaaaatcac atttgatctc agcctataaa
     4381 aggcccccct agtggataaa tattttgttt ttataatgta ttcatcatag catacttcgt
     4441 gaaactgtca aaagaattag ttatataatc tccttaaaac ccttcttcat ttctaacttt
     4501 taatgatgtt tcaataatca tatctaatag tttgctataa ctaattcccg ctgcttctgc
     4561 acttttgggc aatagacttg attgtgtcat ccctggtaat gtattaattt ccattacata
     4621 tggaattccg tccttcacca tcatatcaac tcttgcataa acactacatt ttaatgcttt
     4681 ataacaagcc agtgaagctt tatttacacg ttcattaatt tctgctggaa gttcgataac
     4741 ttcctcaact gtactagtat catcatattt tgcattgtaa tcaaagaact cggctgcatg
     4801 tcgaattgaa actataggta actgttttcc atccagaata gaacatgtaa tttcatcacc
     4861 ttttatatac ttctcaatta ctacttcaga atcccattcg aatacagttt ctagcattga
     4921 gatcaattca tttttattgt aaacgatttt caccccgaca ctcgatccgc cggagtttgg
     4981 ttttaccact aatggaaatc ctaacttatc taactcatca agatttagat cctccatttt
     5041 tgtaagttca atccaatctg gtgtttctac accttcataa cgtaaaatct ttttcgaaat
     5101 atttttatcc atacatatac tactagataa catattactt ccgctataag gaatccctaa
     5161 actctccagt gttccttgaa ctgtcccatc ttctccgtat tttccgtgta atgcgagcaa
     5221 tgcaaagtca atgtctttcg ctttttcaat taaatccatt ttttcattta atgtaattgg
     5281 gactatttca tacttatttt tatctaaatg agcaatcatt tcattcccgg tcataattga
     5341 aacttgtttt tcagaagata ccccgcccat aataacaccg attttcattt tctcaactcc
     5401 aattttcatt ttttaatgta tcaccaatta ttttaattcc ccgaactatt tcttcttctt
     5461 tcaagcgcga aaaccctaat cggaaagtgt tcgctccttc tccatcagag taaaaaacat
     5521 ctccaggtga aaacgtaacg cctttctcat aacacttttg taaaagtgta cgggcgttca
     5581 tatgctcttc tagctctata aataaatgca gtccaccatc tccagtcatt cttttgaaag
     5641 gaatgtactg gttacatgcc ccgacagcta actcatattt tttcttataa acagatcttg
     5701 ccttttttaa atatttttca aaatatccct catgtaaata ttgaaacaga acagcttgat
     5761 ctagcgtaga tgtgtgaatc gttcttgctc tttttacgct ttctaaataa tgaatcagct
     5821 ctttatctgc aataatccac cctacacgta aaccagggaa aagtaccttt gaaaagctac
     5881 taatataaat cacattattg ccagcaccag caaaagttaa taatggagct aaatgtgaac
     5941 ctgaataacg taattcttca ttaaatccgt cctctataat aggaacgttg tattttgaga
     6001 ataacctcat caattccgtt cttttctctg aactcgtaac aataccggtt ggattatgat
     6061 aagaaggaat taaataagca aaatcaaaat ctttttcacg caaacttttt tcaacttcat
     6121 tcgtatcaat accatcctca tgcatatcaa tcccatgaac ttcaagtcca tgtaaacgga
     6181 aaagcttaag tgcagcatgg tgagttggat tctcacaaat gacacgaccg gatttttttg
     6241 ataaagaaga gagtacaata tccaatcctt ctgtaaatcc atttgtaatt agaatatctt
     6301 tattcgaaat gtctacacct ttcatttcca tgtaatgaag taaataattc attaacggtc
     6361 tatatccctt tgcatatccg tagttcaata cgatgtcccc ttcaatggac atacgagtaa
     6421 gaaaagctct tttgaaattt tcaacatcaa atagcttctc atccggggct atactattaa
     6481 aaacaatcat tcccttttcc caacgaacac catgtttcat taaatctaat tcgtccgcta
     6541 atgcggtcac tgtattaagt ttatttttcc aatctatttc aacagacgaa gtgttagata
     6601 tatccacctt cgcaacaaag ttcccttttc ctttaactgc ataaatgagt ccttcttgct
     6661 ctaaatctgc ataagcagcg agtacagtgt ttctactcac acttagtaat tcacttagtt
     6721 cccttgttga tgggattttt tgatccccga gcaaatgccc tttcataatc atctttttca
     6781 aatagtcttt taattgaata taaacgggac gatctttaac aactttaaaa tccttaaaca
     6841 attccttacc ccctgcaatc atttttgcat atttatttca ttctcaaaag gtccacgaca
     6901 tatgtatttt ttataaaggg cggtacggtc ttacaaatct tttcgatata agttaattac
     6961 atcttttaaa gctgcagctg cagcgcctcg tggattagaa gccccaccaa ttgtagtaac
     7021 ttgtcccatc gtatctgtaa agaacgtaat tcctttttcg gtcccattta catttgctaa
     7081 cggatgatct ttttctattt cgcacggttc aacatttaaa ttcacattgc caccctcatc
     7141 cttatatatt gatgctatta atttaataat tttatgttgt ttcttagcat tttgtatttg
     7201 ctgttttgta acgtgctcga ttccttttat atgtatatca gtaagtgttt tctctgtttc
     7261 cattaagctg ttagtcaaaa gtaa
//
