LOCUS       NZ_NTSF01000011        10249 bp    DNA     linear   CON 02-APR-2024
DEFINITION  Bacillus thuringiensis strain AFS021634
            AFS021634_110_H4_Contig11_140523, whole genome shotgun sequence.
ACCESSION   NZ_NTSF01000011
VERSION     NZ_NTSF01000011.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 308991)
  AUTHORS   Bleich,R.M., Kirk,G.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 308991)
  AUTHORS   Bleich,R.M., Kirk,G.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Assembly Cell v. 4.2.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 48.88x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002563165.1-RS_2024_04_02
            Annotation Date                   :: 04/02/2024 02:22:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,903
            CDSs (total)                      :: 5,824
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 84 of 212
            Pseudo Genes (incomplete)         :: 133 of 212
            Pseudo Genes (internal stop)      :: 67 of 212
            Pseudo Genes (multiple problems)  :: 60 of 212
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:49:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 42228
            Orig. end                         :: 52477
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NTSF01000011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10249
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10249
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10249
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5249](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5249
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    365..374
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 17.8"
                     /tool="antismash"
     gene            989..2350
                     /locus_tag="CN325_RS12010"
                     /old_locus_tag="CN325_12015"
     CDS             989..2350
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017763222.1"
                     /locus_tag="CN325_RS12010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_12015"
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_098390811.1"
                     /transl_table=11
                     /translation="MNGKRKIFTCISIVGIGLASFSNSSFAASVTDNSIQNSIPVVNQQ
                     VAAAKEMKPFPQQVNYAGVIKPNHVTQESLNASVRSYYDNWKKKYLKNDLSSLPGGYYV
                     KGEITGDADGFKPLGTSEGQGYGMIITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNL
                     MGWVVADSKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGTVNYLKEAQDMITKGIKAS
                     NVTNNNRLNLGDWDSKSSLDTRPSDWMMSHLRAFYEFTGDKTWLTVINNLYDVYTQFSN
                     KYSPNTGLISDFVVKNPPQPAPKDFLDESEYTNAYYYNASRVPLRIVMDYAMYGEKRSK
                     VISDKVSSWIQNKTNGNPSKIVDGYQLNGSNIGSYSTAVFVSPFIAASITSSNNQKWVN
                     SGWDWMKNKRESYFSDSYNLLTMLFITGNWWKPVPDDKKIQNQINDAIYEGYDN"
     gene            2513..3436
                     /locus_tag="CN325_RS12015"
                     /old_locus_tag="CN325_12020"
     CDS             2513..3436
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="CN325_RS12015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_12020"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_098390812.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATKPLTEEITTYYPEIHGAEGLGPIHVPENVLSIPIYNF
                     GSIAAILIKYGKDLTIVDVGRSTSLAIAFNLWNDLMLNVKGIYFMGGVFLEVGNVTPLA
                     EANVYGDPIASKIVFHQAKNLFIFPLNVTNKAVLTPNVFNFIQANAKNPFHMIMKPMYD
                     YYLSAYQKLNPSMEGPLLHDVVAISGLVNPSFFEFVHRTVNVDTYGDTKGQTFADFRPS
                     SISDGAHIALEIDEQKFIQDFIKIML"
     gene            3513..3971
                     /locus_tag="CN325_RS12020"
                     /old_locus_tag="CN325_12025"
     CDS             3513..3971
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="CN325_RS12020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_12025"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_098390813.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRENWEEALKLQVKGNQLKFVPSVAVSLAKVYIKPDG
                     DKVEYIPFAIYDGDLMVGFIMHAVVIETSDMYWINGFIIDQSQQDNGYGKAALQESINI
                     IKNTFKSCKEIRLTVHKDNISAKKLYERYGFISLGQEYDGEQVYRLFV"
     gene            4006..4338
                     /gene="cutA"
                     /locus_tag="CN325_RS12025"
                     /old_locus_tag="CN325_12030"
     CDS             4006..4338
                     /GO_process="GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="cutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002041227.1"
                     /locus_tag="CN325_RS12025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_12030"
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="WP_098390814.1"
                     /transl_table=11
                     /translation="MKIEVFIPEEYIETLRDELNKIGACKTGEYDHCLSYSSVKGYWRP
                     LDEASPFNGEIGRICEGQECKVEIKCKRELVKDALEVINDIHPYETPMIYIIPILNDYF
                     EQLYIE"
     gene            4355..4801
                     /locus_tag="CN325_RS12030"
                     /old_locus_tag="CN325_12035"
     CDS             4355..4801
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="CN325_RS12030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_12035"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_098390815.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAVMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LIRELREELGVLCSIQQFLGVIENQWQDKEVLHHEINHIFEVESQGLHTDLTPHSSESH
                     LAFHWIDYSKESLNTHKIMPTPSVKELLERKLSDELLNCWISNF"
     gene            5001..5249
                     /locus_tag="CN325_RS12035"
                     /old_locus_tag="CN325_12040"
     CDS             5001..5249
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071345.1"
                     /locus_tag="CN325_RS12035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_12040"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001071345.1"
                     /sec_met_domain="TIGR03601 (E-value: 3.1e-44, bitscore:
                     143.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQHELQTLNLNDYQTGNVVYWDQQQSQYPYYYIQGDARRCGG
                     CGGCGGRCGGCGGGRCGGCVGCAGCFGCFNCWNWWII"
     gene            5351..6366
                     /locus_tag="CN325_RS12040"
                     /old_locus_tag="CN325_12045"
                     /pseudo=""
     CDS             5351..6366
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="CN325_RS12040"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_12045"
                     /product="serine hydrolase domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MYTYEKLTSWVEDIKEKNHSSAAAICIIKNNKVVLEHYSGYHSNT
                     STNKKATASSQFNVASARKSYLGLMIAYALYEGKINSIDDEAIKYFKDFNPALLGKTTI
                     RHLVTHSHGLEEMNDGTIFREFEPGQAWAYRDINVRMMTGLIYQLYNKSFPELLKERVF
                     KPANFQETGWRVQRDENLVDVVNNPNEDAISEIGTVDDGTEKICLSQLENLLIGAIFI"
     gene            6398..6541
                     /locus_tag="CN325_RS30920"
                     /old_locus_tag="CN325_12050"
     CDS             6398..6541
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="CN325_RS30920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_12050"
                     /product="hypothetical protein"
                     /protein_id="WP_000653680.1"
                     /transl_table=11
                     /translation="MKDKVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            6821..8029
                     /locus_tag="CN325_RS12050"
                     /old_locus_tag="CN325_12055"
     CDS             6821..8029
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="CN325_RS12050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_12055"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_098390816.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAAKLQKKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLIDNLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNAVTETLNNDYAPREMMRTKQVYLLFFMLFTS
                     CMGGLYLISMVKDIGVQLVGLSAATAANAVAMIAIFNTVGRIILGTLSDKIGRMKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGAILGGFQPTFIIIGVLSVISFIISILIRPPNAEKKKELKHLHR
                     KVA"
     gene            8160..8585
                     /locus_tag="CN325_RS12055"
                     /old_locus_tag="CN325_12060"
     CDS             8160..8585
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706722.1"
                     /locus_tag="CN325_RS12055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_12060"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_098390817.1"
                     /transl_table=11
                     /translation="MKIVQQWVQEDSDYIREKVIGYNQKYLADEEKTPSEKVSFIVRNE
                     KEEIVGGVTAITFWHHVHVNFLWVSEEYRHEGYGSKLIKLIEEFAIEKGCRLINLDTFS
                     FQAPDFYKKHGYKVIGVSEDHPKGHNHYYLEKRLKSI"
     misc_feature    complement(8766..8784)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.4"
                     /tool="antismash"
     gene            8865..9308
                     /locus_tag="CN325_RS12060"
                     /old_locus_tag="CN325_12065"
     CDS             8865..9308
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="CN325_RS12060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN325_12065"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_000757601.1"
                     /transl_table=11
                     /translation="MKKVNVTYAILYDKTNEKILMVKNKGENGSYYTLPGGAVKLGETL
                     EEAVIREVKEETGLHINVKGICSISEAFFEERDHHAIFFNFLGEIIGGETYISRPKEIE
                     EITWMELHIAAPYLRIPEHLLDLLQKKETVPYFFNGTIVHQSS"
     misc_feature    complement(9491..9504)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.32"
                     /tool="antismash"
ORIGIN
        1 aactgctttt ccagcaattc gtatggggct tgaatcttat acgcctgaac accttacttt
       61 actacgctta ttaatcgcct catttatact tttgttattt tcatttatat acaaactacg
      121 actaccagat ttaaaggata tcccagcaat ttttatattt ggtgctctag gatttactat
      181 ttatcacatc gcattaaact acggcgaaaa aacagtaaat gctggatctg caagtttaat
      241 aatttcagtc actccaatag tgacggcaat tcttgcttct gtttttttga atgaaaaaat
      301 gaaattaaat gggtggatcg gtggtgtaat gagttttgca ggaattgcct taatttcatt
      361 tagcgaaggc gacgctattc aattgaatag cgggggatta tttatattat tagcggcgat
      421 ttcagaaagt ctatttttcg ttttccaaac atcttactta aaaaaatacg gcttcttacc
      481 atttaccata tatacaattt tgtctagtac tgtatgcatg cttattttct tacccggagt
      541 ttatcaaaaa atactagcag ctcctcctga agttaacttg agcgttatat atttaggtgt
      601 ctttccaaca gtacttccgt atatagcatt ggcctatatt atatctcgtg ctggtgcttc
      661 tgaagcaaca agttctctat atttaacgcc aataactgcg tgtttcattg cttggatatg
      721 gttagaagaa gtgccaactt tagtttcgat aattggcgga ggaattacta tacttggaat
      781 tgtgattgct catttaccag tattaagaaa agaaaaacat aggaatttaa caaataatca
      841 atccgtataa tgtaattcaa taatgatttt atgatacttt tttatatagt ttctttctta
      901 tgttgataaa tataatattt tgtatttcag ttaaatattg tatgttacat tttatgtagt
      961 aagcatttta aaaggagctg acaacataat gaatggaaaa agaaaaattt tcacatgtat
     1021 ttctattgta ggaatcggac tagctagttt ttctaattct agtttcgcag caagtgtaac
     1081 ggacaattca atacaaaatt ctattcccgt agttaatcaa caagtagctg ctgcaaagga
     1141 aatgaaacca tttccccagc aagttaatta tgcaggtgtt ataaaaccga atcatgttac
     1201 acaggaaagt ttaaatgctt ctgtaagaag ttactacgat aattggaaaa agaaatattt
     1261 gaaaaatgat ttatcttctt tacctggtgg ttattatgta aaaggagaga ttacaggtga
     1321 tgcggatggg tttaagccac ttggaacttc agaaggtcaa gggtatggga tgataattac
     1381 agtattaatg gctggttatg attcgaatgc tcaaaaaatc tatgacggtt tatttaaaac
     1441 agcaagaact tttaaaagct ctcaaaatcc taatttaatg ggatgggttg tcgcagatag
     1501 taaaaaagca caaggtcatt ttgattctgc tactgatgga gatttagata ttgcgtattc
     1561 tcttcttctt gctcataagc agtggggatc taatggaaca gttaattatt tgaaagaagc
     1621 acaagacatg attacaaaag gtattaaagc tagtaatgtt acaaataata accgactaaa
     1681 tttaggagat tgggattcta aaagttcact tgatacgaga ccatctgatt ggatgatgtc
     1741 acaccttaga gcattttatg aatttacagg tgataaaact tggcttactg ttattaataa
     1801 tttgtacgat gtttatacgc aatttagtaa taagtactct ccaaatacag gacttatttc
     1861 agatttcgtt gtaaaaaacc caccacaacc cgcacctaaa gacttcttag atgagtcaga
     1921 atatacaaat gcatattatt acaatgctag tcgggtacca ttgagaattg taatggacta
     1981 tgcgatgtac ggcgagaaaa gaagtaaagt catttctgat aaagtctctt catggattca
     2041 aaataaaacg aatgggaatc cttctaaaat tgtggatggt tatcaattaa acggatctaa
     2101 tattggtagt tattcaactg ctgtatttgt ttcaccgttt attgctgcaa gtataacaag
     2161 tagcaataat caaaagtggg taaatagcgg ttgggattgg atgaagaata agagagaaag
     2221 ctattttagt gatagttata atttattaac tatgttattt attacaggaa attggtggaa
     2281 acctgtacct gatgataaaa aaatacaaaa tcaaataaat gatgcaattt atgaaggata
     2341 tgataattaa ttaaatttga gaatggttac ttaagaaagg aattagcata cgctagttcc
     2401 tttttaatat tttgtaaaac attatccatt caaaaattag catagatata cttgcttttt
     2461 tcatatacat agttattgat gactctatgt ttttgtgagg ggagagtatt tcttggaaaa
     2521 agtattattt ttcggagacc caggtattga tgactcattt gccattatgt atggtttact
     2581 gcatcccgaa attgaaattg tcggtattgt aaccggatac ggaaatgttg agcatataca
     2641 tgctgcacat aatgcagcgt atattttaca gttagcaaat agacaagaca ttcctgtcat
     2701 tagtggtgca acaaaaccac ttacagaaga aattactacg tactatcctg aaattcacgg
     2761 agcagaagga ttagggccta tccatgttcc agaaaatgta ttatctattc caatatataa
     2821 tttcggcagt attgctgcga ttcttataaa gtacggaaaa gatttgacga ttgttgatgt
     2881 aggaagatct acctcgttag caattgcttt taatttatgg aatgatttaa tgctaaatgt
     2941 aaaaggaatc tatttcatgg gtggtgtttt tttagaagta ggaaatgtta caccattagc
     3001 agaggcaaac gtgtatggag atccgatagc aagtaaaatt gttttccatc aagcaaagaa
     3061 tttattcata ttccctttaa atgtaacaaa caaagctgtg ttaacaccaa atgtatttaa
     3121 ctttattcaa gctaatgcaa agaatccatt tcatatgata atgaaaccaa tgtatgacta
     3181 ctatctatcc gcataccaaa aacttaatcc atctatggaa ggacctttgc tacatgatgt
     3241 ggttgcaata agtggattag taaatccaag tttttttgaa tttgtgcatc gtacagtaaa
     3301 tgtagataca tacggagata cgaaaggaca aacatttgct gattttcgcc ctagctctat
     3361 atctgatgga gcccatatag cactagaaat agatgaacaa aaattcattc aagattttat
     3421 aaaaatcatg ttataagttg ttgtcgaaaa agggaattat ttcactacgt tgaatataaa
     3481 catacatcct ctatataagg gagcttacaa tattgaatgt ccaattaaag gttgttacga
     3541 gagaaaattg ggaagaagca ttaaagctac aagtaaaagg aaatcagttg aaatttgttc
     3601 catccgtagc agtttcgctt gctaaagtat atataaaacc agatggtgac aaggtagagt
     3661 acataccatt cgctatatac gatggtgacc ttatggttgg ttttattatg catgctgttg
     3721 taatagagac atcggatatg tattggatta acggatttat tattgatcaa tcgcagcaag
     3781 ataatggtta tggaaaagca gcattacaag aaagtattaa tataataaaa aatactttta
     3841 aatcgtgtaa agaaattaga ttaacagtac ataaagataa tatctctgca aagaaactat
     3901 atgaacgata tggtttcata tcattaggac aggagtatga tggtgagcaa gtatatcgtt
     3961 tatttgttta actattagga agtaaaggtg tgacaattta ctgaagtgaa aattgaagtg
     4021 ttcattcctg aagaatatat tgaaacatta agggatgaat taaataaaat tggagcatgt
     4081 aaaacgggcg aatatgacca ttgtctttcc tatagttcag tgaaaggata ttggagacct
     4141 ttagacgagg catctccttt taatggagaa attggtcgaa tatgcgaagg gcaagaatgt
     4201 aaagttgaaa taaagtgtaa acgagaactt gtaaaagatg ctcttgaagt tattaatgat
     4261 attcatccgt atgaaacacc tatgatttat attataccaa tactaaatga ctattttgag
     4321 caactataca ttgaataact aggagggata tcaattgaaa agtaaattcc atcatattgt
     4381 acgagcagtc atgataaaag atgaaaaatt attagttgct gaatatatcg gccatcatta
     4441 ttttttacct ggtggccatg ttgaaattgg agaatcagca gagaacgcat taataagaga
     4501 gttaagagaa gaacttggag tactctgtag tatacaacaa tttttaggag tcatagaaaa
     4561 tcaatggcaa gataaagaag tgcttcatca tgaaattaat cacatctttg aagtagagtc
     4621 acaaggttta catacggatt taacaccaca ctctagcgag tctcatttag catttcattg
     4681 gatagattat agtaaagaat ctttaaatac tcataaaatc atgccaacgc cttcagttaa
     4741 agaattacta gaaagaaaat taagtgatga actattaaat tgttggataa gtaattttta
     4801 aatcactcta tttatagtaa ataggtccat cgtaaggata taaagaaaat aggctaaaaa
     4861 acaaggtaca tcacctagtg taagttcata ttttgttgat gtacgcattt agtctatagt
     4921 cttgtttcta ttttgtttac aaaaatatat aaactttaac gtttgggcga aatttatatt
     4981 tataaatgga ggggttcgta atgaatcagt ttcaacatga attacaaaca ttaaacctta
     5041 atgattatca aactggtaat gttgtgtatt gggatcaaca acaaagtcaa tatccatatt
     5101 attacatcca aggcgatgca cgtcgttgtg gcggatgcgg aggttgtggt ggacgctgtg
     5161 gcggttgcgg cggcggtaga tgtggtggtt gcgtaggttg tgccggatgt tttggttgct
     5221 ttaattgctg gaactggtgg attatttaaa tatagtacat ctttaatatt ttatatgaaa
     5281 gaaaggtgta aatactgttt atacttttct ttcatgctgt aattttgtat taggattaag
     5341 gaggatttat ttgtatacat acgaaaagtt gacttcttgg gtagaggata taaaagaaaa
     5401 aaatcatagt tctgcagcag caatttgtat tataaaaaat aataaagtcg tattagagca
     5461 ttatagcggt tatcactcaa atacatcgac taataaaaaa gcaaccgcat cttcgcagtt
     5521 caacgttgct tctgctagaa aaagttattt aggattaatg atagcgtatg cgctttacga
     5581 ggggaaaata aactctattg atgatgaagc gataaaatat tttaaagact ttaatcctgc
     5641 attgcttggt aaaacaacga taagacattt agtaacacat tcgcacggat tagaagaaat
     5701 gaatgacggg acaatttttc gtgaatttga accaggacaa gcatgggcat atagagatat
     5761 taatgtaaga atgatgacag gtcttattta tcagctatat aacaaaagtt ttcctgaatt
     5821 gttaaaggag cgtgttttta agcctgctaa ttttcaagaa acaggttgga gagttcagcg
     5881 agatgaaaat ttagttgacg ttgttaataa tccaaatgaa gacgcaatta gtgaaattgg
     5941 tacggtagat gacggtactg aaaaaatctg tttgtctcag ctagagaatt tgcttattgg
     6001 ggcaatcttc atttaaatca aggtgtgata aacggtaagc aaattgttcc aaaagaagtt
     6061 ataaaaatcg ctacgagttt gcagagtcca acatatgtaa ataaagagct accacaaaat
     6121 ggcttgtttt ggtttgttca aaatgaacct gcacaattaa gcgaacttgg tgaacgtgtt
     6181 ccaaaagggt cgtatcaaat attagggatt actggaccga ctattttagt aatacctgaa
     6241 tataatgtag ttgttgcaaa aatgtataac aaaagatata actacggcgg taataattac
     6301 ttatattatt tacgtgaatt tagtaattta gtcgctgata catttagtaa cgttaatagg
     6361 gcataattac tcctataaca aaaaggagtg attgaaattg aaagataaag tgaacaaaaa
     6421 taaaaaacaa aatataaaag atgtaaatac agagcaaaat gcaatttata gcgatcctaa
     6481 agatgcagct aatatgcaaa ctgtaccgca gccgaaggat tttgatgaaa tcgaatatta
     6541 atttttcatc cgactatatg atgaatgaaa aatacgtctt aagtctcatc acatataaga
     6601 aattatttga taacatatat acataagcat ttgaaaatgg cttatcttaa caaagctttc
     6661 tttagaaaca tctgatataa cctgcaaaag acacttcttt atgaagtgtc tttttgtgta
     6721 tatgaactta attaccattt tccgtacagt atcttgcaat ttacgatgga tatactaact
     6781 gtgaaaatcc atgtcaagat aaaagaaggg gacagttagt atgaaacaaa cttctataaa
     6841 tccgttacta attgttctag gtacaatcat tgttcaaatt ggccttggaa caatttatac
     6901 atggagttta tttaatcaac cccttgtaag taagtttgga tggaatctta attcagtagc
     6961 gataactttc tctataacaa gtttttcttt atcattctca actttatttg cagcaaaatt
     7021 gcagaaaaaa ttaggacttc ggaaacttat tgctactgca ggaattgttt taggactcgg
     7081 cttaatactt agttcacaag tttcttcctt accactctta tatttattag ctggtgtcgt
     7141 tgttggttat gcggatggaa cagcttatat tacatcacta tctaatttaa ttaaatggtt
     7201 tccaaatcgg aaagggctta tttcaggtat atctgtatca gcttatggaa tgggcagctt
     7261 aatctttaga tatataaacg gaaatcttat cgataatctt ggtgtatcac aagcattctt
     7321 atattggggc attatcgtgt tacttttagt attaatcgga tcgttcttct tacgtgaggc
     7381 aatcgtaagt aatgctgtaa ctgaaacatt aaacaatgac tatgctccgc gtgaaatgat
     7441 gcgaacgaaa caagtgtatc tgctattttt tatgttattc acctcgtgta tgggtggttt
     7501 gtatttaata agtatggtaa aagatatcgg tgtacaactc gtcggactta gtgcagcaac
     7561 cgctgcaaac gccgttgcta tgattgcaat ttttaataca gtaggtagaa tcattcttgg
     7621 gacgttatca gataaaatcg gccgaatgaa aattgtctct gcaactttta ttattatagg
     7681 tttgtcagtc tttactttaa gttttattcc gctaaattac ggaatctatt ttgcttgtgt
     7741 agcaagtgtc gccttttgct tcggcggtaa tataactata ttcccagcta ttgtcggaga
     7801 tttctttgga ttaaaaaacc atagtacaaa ttatgggatt gtctaccaag gtttcggatt
     7861 tggtgcgctt gcaggatcat ttattggagc gatactcgga ggatttcaac caactttcat
     7921 tataatcggt gttttaagtg ttatttcctt cattatttca atattaatcc gcccaccaaa
     7981 tgcagagaag aaaaaggaac taaaacattt acatcggaaa gtagcttaat tttaaaaaga
     8041 gtcctcatgt tagggctctt ttttattcca ttaactttgc ttctttacga acttattaaa
     8101 gacaggacaa tttcattata aaattaaata gataagtaaa aaggagcgtg acacttaaaa
     8161 tgaaaatagt acagcagtgg gtacaagaag atagtgatta cataagagag aaagtgattg
     8221 gatacaatca aaaatatctt gcagatgaag aaaaaacacc ttcagagaag gttagtttca
     8281 tagtaagaaa tgaaaaagaa gaaatagtag gtggggtaac ggcaataact ttttggcacc
     8341 atgttcatgt taactttcta tgggtttctg aagaatatag gcatgaaggt tacggaagca
     8401 agttaataaa gcttattgaa gaatttgcaa ttgaaaaagg gtgtaggtta attaatttag
     8461 atacttttag tttccaagct cctgattttt ataagaaaca tggatataaa gtaattggag
     8521 taagtgaaga tcatccgaaa ggacataatc attattattt agaaaaacgg ttaaaaagta
     8581 tatagaattt gttaacgtta gtatttgaaa tacaaatgca ataagggcga ggttattatt
     8641 aatttcagta ttaattgaat caatttttat actaaaactg caagaacgta ctttggataa
     8701 tcttcctaag gtacgttctt tttttatatc agtagcctat tcttattaac tccaacaata
     8761 gcggtaaagg caagacttaa ctaacttata tatattaaaa ggagttaatg gtagaatgaa
     8821 cagaagacag aaaattcctt ttatttaaaa tagaggaggt acatatgaaa aaagttaatg
     8881 ttacatacgc gattttgtac gataaaacaa acgaaaagat cttaatggta aaaaacaaag
     8941 gagaaaatgg ttcttattat acattaccag gtggcgcagt taaattagga gaaacgttag
     9001 aagaagcagt aattcgagaa gtaaaagaag aaacagggtt acatataaac gtaaaaggga
     9061 tttgttctat cagtgaagcc tttttcgagg aaagagatca tcatgcaatt ttctttaatt
     9121 ttctaggcga aataatcggc ggggagacat atatatcaag accaaaagaa atcgaagaga
     9181 ttacttggat ggaattacat atagcagcac cgtatttacg cataccagaa catttactag
     9241 atttattaca aaagaaagaa acagtacctt atttttttaa cggaacaatc gttcatcaat
     9301 cttcataaaa atattatctc atgtaaggcg gggaaaacat ggagttcatc gttaatcaca
     9361 aacaatttac tcaagcactt tcagaagtta acaaagcgat atcaacaaaa tctttaattc
     9421 ctatattatc tggtataaaa ataactgcag atcaatctgg aattacttta attgcaagta
     9481 actcgaatat ttttattgaa aaattcattc ctgtttcaat tgaagacgaa aaaattgtaa
     9541 ctattttaaa ggcaggaact attgttgtac ctacaaagta tttcattgaa ataataaaga
     9601 aaatgccaag tgatatagca ataaaaagta agaatgagca aattattaag atacaatcag
     9661 aagaaatcac attaagttta aatggatttc ctgcagatga gtttccgaat gtaccgctta
     9721 tagacaatca tgcagaaatc aaggtagaga cagagcaatt gattgaagta tttaaacaaa
     9781 cagtctttgc ggtagcgaaa aatgaatcta gacctgttct tactggggtg catatagagt
     9841 tatctaataa taagtttatt tgtgctgcaa ctgactcaca tagactagcg atacgtgaaa
     9901 cgcgactttc ctcagatgta aaagcaaatt gtattgtacc aagcgcaacc attagtgaac
     9961 ttctaaaatt aatgaacaac aattcggaat tcgtatatat ttacctttca gagagtcata
    10021 ttatatttac gcttggaaca actacattat attcaagact aattgaaggg aaatatccta
    10081 atatttctaa ccttattcca aatgacttta aaacgattat taacatagat agaaaaaaga
    10141 tattacaagg tgtagaccga tcaagtttat tagcaagtga atgggccaat aacaatgtta
    10201 acttagaaat cataaacgaa tctacaatta aaatttcttc aaacgcttc
//
