LOCUS       NZ_NTWJ01000051         8931 bp    DNA     linear   CON 01-APR-2024
DEFINITION  Bacillus thuringiensis strain AFS009835
            AFS009835_104_C9_Contig51_140523, whole genome shotgun sequence.
ACCESSION   NZ_NTWJ01000051
VERSION     NZ_NTWJ01000051.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 81578)
  AUTHORS   Bleich,R.M., Kirk,G.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 81578)
  AUTHORS   Bleich,R.M., Kirk,G.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Assembly Cell v. 4.2.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 61.85x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002562205.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 01:49:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,978
            CDSs (total)                      :: 5,906
            Genes (coding)                    :: 5,721
            CDSs (with protein)               :: 5,721
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 82 of 185
            Pseudo Genes (incomplete)         :: 109 of 185
            Pseudo Genes (internal stop)      :: 57 of 185
            Pseudo Genes (multiple problems)  :: 52 of 185
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:13:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8931
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NTWJ01000051.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..8931
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..8931
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..8931
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3610:3931](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(3611..3931)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(18..32)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 19.98"
                     /tool="antismash"
     misc_feature    19..33
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 18.69"
                     /tool="antismash"
     gene            471..1580
                     /locus_tag="CN427_RS29835"
                     /old_locus_tag="CN427_29835"
     CDS             471..1580
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1077: phage integrase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675870.1"
                     /locus_tag="CN427_RS29835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_29835"
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_098283028.1"
                     /transl_table=11
                     /translation="MKGHIRKRGNKYCIVIDIGPDPETGKRRQKWFSGYKTKKEAQADV
                     AKKITELNEGTFIEPSKVTLKDYLNHWLEIKSMSIEKSTFAGYRAFINQHVIPSIGMVA
                     LHKLNVMHIQKCYKTTIDKGIASNSILLMHRILKSALNLAVKQNIMSRNPADFAEIPKK
                     EKTPIQTWTEEEVKKFLAHSQESRYHIGYLLAITTGMRLGEVLGLRWQDVDFEKHTVTI
                     NQTSGHDNKIKKTAKTNSSKRTIPVPNETIAALKKHKILINKEKLRFGSAYLDQDLINC
                     NEFGRIIKRAPFRKSFIRATHKVGIKEIKFHDLRHTHATILLKQGVNPKIISERLGHTD
                     ISMTLSVYSHVLPNMQEEAVKNFGKSIFG"
     gene            complement(1649..2320)
                     /locus_tag="CN427_RS29840"
                     /old_locus_tag="CN427_29840"
     CDS             complement(1649..2320)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124615.1"
                     /locus_tag="CN427_RS29840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_29840"
                     /product="DUF3962 domain-containing protein"
                     /protein_id="WP_000736204.1"
                     /transl_table=11
                     /translation="MKKLRLLTFENIVEPLLNEKVSFIYFPIEWLDIVEIHYKTFLLTS
                     KLKRLNERLYDMFSDILFIQHNPYVLNENTPWIVSKEPIRQEQLDYIFQSWYEIIHDWK
                     PNRLVEPPKYEWQSDLISNLPVLHDNETYSKWVPALISHIFCERPIYLENTNEEEIYFS
                     PLRSQNICEAMSGPIKDEKTQDFFSYVYRFECITRGGENAPLLNISIGIRRFYQEYKMI
                     G"
     misc_feature    complement(2312..2325)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.3"
                     /tool="antismash"
     gene            complement(2632..3117)
                     /locus_tag="CN427_RS29845"
                     /old_locus_tag="CN427_29845"
     CDS             complement(2632..3117)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124614.1"
                     /locus_tag="CN427_RS29845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_29845"
                     /product="homoserine dehydrogenase"
                     /protein_id="WP_071714876.1"
                     /transl_table=11
                     /translation="MNMIELKSIIHSYKLKRKIAKDLYGKRDELTLLLNEFNKMKCTVT
                     SEKKKNNILSRLQLIYQNMKLDKQYPLSVAFDSKLLERLEKEPLHTIGDGVTCLHLMLD
                     VNYEKIKQYGSSTSRSFVPLSQSSICLADCICLTGVVVGLLGAISFGGLLLSICSIT"
     misc_feature    3149..3167
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.87"
                     /tool="antismash"
     misc_feature    complement(3218..3235)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.24"
                     /tool="antismash"
     gene            complement(3327..>3461)
                     /locus_tag="CN427_RS31100"
                     /pseudo=""
     CDS             complement(3327..>3461)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016077325.1"
                     /locus_tag="CN427_RS31100"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="site-specific integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="YMYVPTKNIAIINGYIQSFK"
     gene            complement(3611..3931)
                     /locus_tag="CN427_RS29850"
                     /old_locus_tag="CN427_29850"
     CDS             complement(3611..3931)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071363.1"
                     /locus_tag="CN427_RS29850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_29850"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001071363.1"
                     /sec_met_domain="TIGR03601 (E-value: 8e-44, bitscore:
                     140.4, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQALSLNDYRSGNIVYWDQQNQYPYYYIEDAARRCGGC
                     GRCGGCGGGRCGGFRCGGFRCIGCFGCFGCGGCGGCGGCGGCSNCFDGFSGTTDTIITY
                     EY"
     gene            complement(4481..4744)
                     /locus_tag="CN427_RS29855"
                     /old_locus_tag="CN427_29855"
     CDS             complement(4481..4744)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101034.1"
                     /locus_tag="CN427_RS29855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_29855"
                     /product="DUF3937 domain-containing protein"
                     /protein_id="WP_002082716.1"
                     /transl_table=11
                     /translation="MNFERCYMFTNKKLIRFGLSLFVFLGIINFTISYFQTYLETAADI
                     KWVIPEIWKTILLDVPQGILVLLGAIALYDFTKEASQKDASI"
     gene            complement(5142..5831)
                     /gene="tenA"
                     /locus_tag="CN427_RS29860"
                     /old_locus_tag="CN427_29860"
     CDS             complement(5142..5831)
                     /EC_number="3.5.99.2"
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="CN427_RS29860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_29860"
                     /product="thiaminase II"
                     /protein_id="WP_098352952.1"
                     /transl_table=11
                     /translation="MTFSQSLRKEVDSIWEASFNHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITKEEKDNFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAVILPCYWLYYEIGERLKECQPEEPIYNEWISAY
                     GSDWFRTLVEEQITRLDTIAGKVTEADRKRMKQHFIISSQYEYSFWEMAYTLEKWAVDT
                     DVKDVIG"
     regulatory      complement(5959..6064)
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     misc_feature    6103..6115
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     gene            complement(6138..7052)
                     /locus_tag="CN427_RS29865"
                     /old_locus_tag="CN427_29865"
     CDS             complement(6138..7052)
                     /EC_number="6.3.2.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="CN427_RS29865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_29865"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_098399378.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 1.2e-23,
                     bitscore: 76.0, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 2.8e-15, bitscore:
                     49.0, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 1.2e-10, bitscore: 33.6,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 6.1e-55,
                     bitscore: 178.1, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 1.8e-07, bitscore:
                     23.2, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRY
                     EGVETPDWIELTKMEDLNLDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVF
                     EWDSEVVIEKYIKGDEITCSILDGKQLPIVSIRHAAEFFDYNAKYDDTSTVEEVIELPA
                     EINKRVNKASLTCYKALKCSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAG
                     ISYSKLLDIIIETSLKVRNEEGF"
     gene            complement(7057..8505)
                     /locus_tag="CN427_RS29870"
                     /old_locus_tag="CN427_29870"
     CDS             complement(7057..8505)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-469). E-value:
                     2.6e-30. Score: 97.7. Matches aSDomain:
                     nrpspksdomains_CN427_RS29870_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="CN427_RS29870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_29870"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_000471607.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 3.6e-30, bitscore:
                     97.6, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYLKKMIMKGHLLGDQKIPSTRELSEL
                     LSVSRNTVLAAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKNKLNTVTALADE
                     LDLMKHGVRWEKGMIVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYKPL
                     MNYLLHYMEMKGVDISNKDILITNGFTEGLDIVLSSLSKKSGRVICENPTHHAALKLFR
                     LHGLEVHGIDMHEDGIDTNEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYKVPIIEDGFNEELRYSGSHLAPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQY
                     IPFKRMTGDGGLHLFIELEEHMNARTLLQKCYEKGVTFSPGDVFYSDGEGANTFRLGFS
                     RLKEEEIVRGIKIIGDTLKNEIWS"
     aSDomain        complement(7099..8070)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CN427_RS29870_Aminotran_1_2.1"
                     /evalue="2.60E-30"
                     /label="CN427_RS29870_Aminotran_1_2.1"
                     /locus_tag="CN427_RS29870"
                     /protein_end="469"
                     /protein_start="145"
                     /score="97.7"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYKPLMNYLLHYMEMKGVDISNKDILITNGFT
                     EGLDIVLSSLSKKSGRVICENPTHHAALKLFRLHGLEVHGIDMHEDGIDTNEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYKVPIIEDGFNEELRYSGSHLAPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQYIPFKRMTGDGGLHLFIELEEHMNARTL
                     LQKCYEKGVTFSPGDVFYSDGEGANTFRLGFSRLKEEEIVRGI"
     misc_feature    complement(8581..8595)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 17.35"
                     /tool="antismash"
ORIGIN
        1 attccggtta ctcccgttgg gcctgtcgga cctgttgctc cacttgctcc cgttggtaga
       61 gtaaatgagg aaatcggtgg caatgtcggt cctattaaac tcgaatccag tgaacttgca
      121 gatagatttt tagagtttaa tccttcccat ttctttttcc tctccatata tctcactcca
      181 aataaaaatc atttcttaat taagaaaata ttaattgtac aaaatatgat atatacaaga
      241 tgtactattc caaataaaat attttaaata aaaatatttc cttaaatata caaaattatt
      301 ttataaaatt aacttttgta aaaaacaacc aatatattaa ataatcaatt ctgttcatat
      361 atggtaaaat atatccatcg ctgatatgtc caactatgta attttcatag cagcaaaatt
      421 aaaactagac ttataccaca tgactcaaaa taaatgaagg agtgttttaa gtgaaaggac
      481 atattcgaaa aagaggaaat aaatattgca ttgttataga tatcggccct gatccagaga
      541 caggaaaaag aagacagaag tggttttctg gatataagac aaaaaaggaa gcacaagccg
      601 atgtggcaaa gaagattaca gagttgaatg aaggaacttt tatagaacca tctaaagtta
      661 cgttaaagga ttatctaaat cattggctag aaattaaaag tatgagcata gaaaagagca
      721 cctttgctgg ctatagggca tttatcaacc aacatgttat acctagtata ggaatggtcg
      781 cgcttcataa attaaatgtt atgcacattc aaaaatgcta taagactacg atagataaag
      841 ggattgcaag caattctatt ctgcttatgc atagaatttt aaaaagcgct ttaaacctag
      901 ccgtaaaaca aaatattatg tctcgaaatc cagcagattt tgctgagata cctaaaaaag
      961 aaaaaacccc tatccagact tggacagagg aagaagtaaa aaagttttta gctcattcac
     1021 aagaatcacg atatcacatt gggtatctac ttgcaataac tacaggtatg cgtctgggag
     1081 aagttttagg tttacgatgg caggatgttg attttgaaaa acataccgtt acaataaatc
     1141 aaacatctgg tcatgacaat aagatcaaaa aaactgcaaa aacaaattct tcaaaacgca
     1201 caattcctgt acctaatgaa acaatagccg ccttaaaaaa acataaaatt ttaattaata
     1261 aagagaaatt aaggtttggt tctgcttatc tagatcaaga tttaataaat tgtaatgagt
     1321 ttggaagaat cataaaaaga gcacctttca gaaaaagttt cattagggcg acacacaaag
     1381 taggtataaa agaaattaaa ttccatgatt taagacatac acacgcaact atactattga
     1441 aacaaggagt taaccctaaa atcatcagtg agagattagg tcatacagat atttcaatga
     1501 cattaagtgt ctattctcat gttttaccga atatgcaaga agaagccgtt aaaaactttg
     1561 gtaaaagcat ctttggataa cgtatgtttg caaaatgttt gcaattcata aaaacaggtc
     1621 aaacaaacgt tgttatatca gggcttgttt aacctatcat cttatattct tgataaaatc
     1681 tccgaatccc tattgaaata tttaataatg gagcgttctc accaccgcgg gttatgcatt
     1741 cgaatcgata tacataggag aaaaaatctt gtgttttttc atcttttatc ggccctgaca
     1801 tcgcctcaca aatattttgt gatctaagag gggaaaaata gatttcttct tcatttgtat
     1861 tttctaaata tataggacgc tcacaaaaaa tatgtgagat taaagcgggc acccacttag
     1921 aatacgtttc attatcatgt agtactggca aattagaaat caaatcggat tgccattcat
     1981 attttggtgg ttctactaat ctatttggtt tccaatcatg aataatctca taccaacttt
     2041 gaaaaatata atcgagctgc tcttgtctaa taggttcttt cgatacaatc catggtgtat
     2101 tttcatttaa tacgtacggg ttatgctgaa taaacaatat atccgaaaac atatcataca
     2161 atctttcatt caaacgtttc aacttactcg ttaataaaaa tgtcttataa tgtatctcta
     2221 cgatgtccag ccattcaata ggaaagtata taaatgatac cttttcattt aaaaggggtt
     2281 ctactatatt ttcaaatgtt agcagcctta attttttcat aaaatgattc cttcctttct
     2341 tcagttgtct tgattatcaa aaagctagaa caatagttac ttaatctcat ccatttaaac
     2401 caagtattta aaagaaacgt aaaaactgtt tatctacata aattattatc aaaccgactc
     2461 ttttaaaaat tcaaagtaat tatcatgtat aacacctcta ttatatttga atttcgatac
     2521 attcgaatta tacattacac ttaatgcatt gtacttatca tttacaatat ctttacaaaa
     2581 aaaccaaaaa agaacacctt aattcggtgc cctttttaca aatatcaacc tttatgtaat
     2641 tgaacatata gataacagta atcccccaaa tgaaattgct cctagtaaac ctactacaac
     2701 tcctgttaaa caaatacaat cagcaagaca aatagaagac tgcgataatg gaacaaatga
     2761 cctacttgta ctcgaaccat attgttttat tttctcataa ttcacatcta acattagatg
     2821 taaacatgtt acaccatccc caatagtatg tagaggttct ttttctaatc gctccaataa
     2881 tttactatca aaagcaactg atagaggata ctgcttatct aatttcatat tttgataaat
     2941 tagttgcaaa cgagataata tattgttttt cttcttttca gatgttactg tacatttcat
     3001 tttattaaat tcatttaata acagtgtcaa ttcatctctt tttccgtata aatctttagc
     3061 tatttttctc tttagtttat acgaatggat aatagatttt agttcaatca tattcataca
     3121 cttcctaatt acatattatt ctcccgatta ttttatagat aaacagtact ttttaatctt
     3181 atttaaatta cactcaagca tcttcattta ctatcgatta acattacatt aaccttacaa
     3241 agttgtaata aattcattag ctagaagaaa tcaatcccta aaccagacag atatttgatg
     3301 ataaaaccat aagaaaaaag caatgattag attttaaatc tagtcattgc tttatccatc
     3361 acctcttgat tacacctata tatcttattg ttactctttc acttgaatga ttgaatatac
     3421 ccattaataa tggctatatt ctttgtcggc acgtacatat ataaaactaa tgttttttaa
     3481 ttgataaggt gaaactttaa ttagccctca ccaattgggc ttttatgggt agtccgccat
     3541 ctaacttctc tttgctcccg ctgaattttt ttagggtctt actgccctgc aaatagcagg
     3601 ataaatgcaa tcaatattca tacgttataa tagtatcagt agtaccacta aaaccatcaa
     3661 aacagttaga gcaaccacca caacctccac aacctccaca acctccgcaa ccaaaacaac
     3721 cgaagcaacc aatacaacgg aatccaccac aacggaatcc gccacaacga cctccaccac
     3781 aaccaccaca acgaccacaa ccgccacagc gacgagcagc atcttcaata tagtaatatg
     3841 gatattggtt ttgctggtcc caatagacaa tatttccaga ccggtaatca ttaagactta
     3901 acgcttgtag ttcttgttga aactgattca ttttcataac ctccgtttat aaaatacaac
     3961 ctcattgatg cttccctata tttctgttcg ttacatctaa gtaaaaatag tgctataaac
     4021 taagttcaat acgtacacca acaaagtatg acttatcact aaatgatgca ccttgttcat
     4081 atacctattt ttgctatggg ctcattttta taaagtgaaa ctttaatcag tgggggggag
     4141 ttcatccccc gctgattatt agtcctcacc aatcaggctt ttacgaacag cccgttaatg
     4201 cgggataaat aaaaattaaa aaatatgaat tctagcgtca tatcttcata cctactaaat
     4261 tcaattaatt tctcaataat tatgttgtca tatcctatat atttccgtct gccgcttaga
     4321 tttttctgta ctgtaaatgc ctttcactat aacacctcca agtaaccggt ttctatgaca
     4381 gataagaaat tcctacaaag aaaaaagccc ttattagggc ttttcattct atttctccag
     4441 caaaactctc tatgaaattc ctaatagaaa aaagacacac ttagattgat gcgtcttttt
     4501 gtgatgcctc ttttgtgaaa tcatataaag caattgcgcc taaaagaaca agtatacctt
     4561 gaggaacatc tagtaaaata gttttccaaa tttctggaat tacccattta atatctgctg
     4621 ctgtttcaag atatgtttgg aaatagctga ttgtgaaatt aattatccct aaaaatacaa
     4681 acaacgataa accaaatcga attaatttct tatttgtaaa catataacac ctctcaaaat
     4741 tcattggata ttcctgcttt tgcttcttta tatttctata tctttatatc taattttaac
     4801 acataaagaa ctcaaaaaaa tacgatatgc atatttgcat atcgtattaa ccacactcca
     4861 tgataattta gaaattcctg caaagaaaaa acaactccgt tacagctaac agctcaggtt
     4921 tcttttctta tttaacttca taccaccaac attaacgttc tttgattgta aaataaatgt
     4981 gacagaagtt tatccagtta atgcgtagac aagtaaatga gctaaattat cgatattcta
     5041 tttcgccata tccgtagcac taatgacttt acgcaaattt gacaagaaaa aatatataga
     5101 taggtatgat ccgtacctat ctataacatt acttctattc tttatccaat tacgtctttt
     5161 acgtctgtat ccactgccca cttttctaat gtataagcca tttcccaaaa tgaatattca
     5221 tattgactgc taataataaa atgctgtttc atacgtttgc ggtctgcctc tgttactttt
     5281 ccagcgatag tatctaaacg tgtaatttgt tcttcaacta gcgtacggaa ccaatcagat
     5341 ccataagcag aaatccattc gttataaatt ggctcttccg gctggcactc ttttaagcgt
     5401 tcgccaattt cataatacaa ccaatagcaa ggtaaaatta ctgcaatgat atctcctaaa
     5461 tgcccttcat acgcagcacg atacatatgt gaagtatacg catatgcggt tggcgcagga
     5521 ataaaattat ctttttcttc ttttgttatc cctagctttt tagcaaaatt ctcatgtaaa
     5581 gataactcgg cctcatatgt attttgagca tgatgtgcca tacgagctgt cgtctctaat
     5641 tcaagagctt ttgcagctcc taaagtttgt actctagcaa aatgactcaa ataatatgaa
     5701 tcttgaagca cataatagcg aaaactagct aaatctaatg ttccttcacc aagttttttt
     5761 acaaaaggat ggttaaaact cgcttcccaa attgaatcta cttctttacg taatgattgt
     5821 gaaaaagtca tttaaatccc tccaataatg tataaaatat aagtatttat ccacaattat
     5881 taaagctgta aaataaaaaa actttcttta caggcgtaaa gaaagtgaaa ttgacagtat
     5941 ttatttactc attaaatagc aacgtctttc ccacttccct acgctggcat taaccagatc
     6001 aggtactaag agtctcaaaa tcacatttga tctcagccta taaaaggctc ccctagtgga
     6061 taaatatttt gtttttataa tgtattcatc atagcatact tcgtgaaact gtcaaaagaa
     6121 ttagttatat aatctcctta aaacccttct tcatttctaa cttttaatga tgtttcaata
     6181 attatatcta atagtttgct ataactaatt cccgctgctt ctgcactttt gggcaatagg
     6241 cttgattgtg tcatccctgg taatgtatta atttccatta catatggaat tccgtccttc
     6301 accatcatat caactcttgc ataaacacta cattttaatg ctttataaca agtcagtgaa
     6361 gctttattta cacgtttatt aatttctgct ggaagttcga taacttcctc aactgtacta
     6421 gtatcatcat attttgcatt gtaatcaaag aactcggctg catgtcgaat tgaaactata
     6481 ggtaactgtt ttccatccag aatagaacat gtaatttcat caccttttat atacttctca
     6541 attactactt cagaatccca ttcgaataca gtttctagca ttgagatcaa ttcattttta
     6601 ttgtaaacga ttttcacccc gacactcgat ccgccagagt ttggttttac cactaatgga
     6661 aatcctaact tatctaactc atcaagattt agatcctcca tttttgtaag ttcaatccaa
     6721 tctggtgttt ctacaccttc ataacgtaaa atctttttcg aaatattttt atccatacat
     6781 atactgctag ataacatatt acttccgcta taaggaatcc ctaaactctc cagtgttcct
     6841 tgaactgtcc catcttctcc gtattttccg tgtaatgcga gcaatgcaaa gtcaatgtct
     6901 ttcgcttttt caattaaatc cattttttcg tttaatgtaa ttggaactat ttcatactta
     6961 tttttatcta aatgagcaat catttcattc cccgtcataa ttgaaacttg tttttcagac
     7021 gataccccgc ccataataac accgattttc attttctcaa ctccaaattt cattttttaa
     7081 tgtatcacca attattttaa ttccccgaac tatttcttct tctttcaagc gcgaaaaccc
     7141 taatcggaaa gtgtttgctc cttctccatc agagtaaaaa acatctccag gtgaaaacgt
     7201 aacgcctttc tcataacact tttgtaaaag tgtgcgggca ttcatatgct cttctagctc
     7261 tataaataaa tgaagtccac catctccagt cattctttta aaaggaatgt actggttaca
     7321 tgccccgaca gctaactcat attttttctt ataaacagat cttgcctttt ttaaatattt
     7381 ttcaaaatat ccctcatgta aatattgaaa cagaacagct tgatctagcg tagatgtgtg
     7441 aatcgttctt gctcttttta cgctttctaa ataatgaatc agctctttat ctgcaataat
     7501 ccagcctaca cgtaaaccag ggaaaagcac ctttgaaaag ctactaatat aaatcacatt
     7561 attgccagct cccgcaaaag ttaataatgg agctaaatgt gaacctgaat aacgtaattc
     7621 ttcattaaat ccgtcctcta taataggaac cttgtatttt gagaataacc tcatcaattc
     7681 cgttcttttc tctgaactcg taacaatacc ggttggatta tgataagaag gaattaaata
     7741 agcaaaatca aaatcttttt cacgcaaact tttttcaact tcattcgtat caataccatc
     7801 ctcatgcata tcaatcccat gaacttcaag tccatgtaaa cggaaaagct taagtgcagc
     7861 atggtgagtt ggattctcac aaatgacacg accggatttt tttgataaag aagagagtac
     7921 aatatccaat ccttctgtaa atccatttgt aattagaata tctttattcg aaatgtctac
     7981 acctttcatt tccatgtaat gaagtaaata attcattaac ggtttatatc cctttgcata
     8041 tccgtagttc aatacgatgt ccccttcaat ggacatacga gtaagaaaag ctcttttgaa
     8101 attttcaaca tcaaatagct tctcatccgg ggctatacta ttaaaaacaa tcattccctt
     8161 ttcccaacga acaccatgtt tcattaaatc taattcgtcc gctaatgcgg tcactgtatt
     8221 aagtttattt ttccaatcta tttcaacaga cgaagtgtta gatatatcca ccttcgcaac
     8281 aaagttccct tttcctttaa ctgcataaat gagtccttct tgctctaaat ctgcataagc
     8341 agcgagtaca gtgtttctac tcacacttag taattcactt agttcccttg ttgatgggat
     8401 tttttgatcc ccgagcaaat gccctttcat aatcatcttt ttcaaatagt cttttaattg
     8461 aatataaacg ggacgatctt taacaacttt aaaatcctta aacaattcct taccccctgc
     8521 aatcattttt gcatatttat ttcattctca aaaggtccac gacatatgta ttttttataa
     8581 agggcggtac ggtcttacaa atcttttcga tataagttaa ttacatcttt taaagctgca
     8641 gctgctgcgc ctcgtggatt agaagccccg ccaattgtag taacttgccc catcgtatct
     8701 gtaaagaacg taattccttt ttcggtccca tttacttttg ctaacggatg atctttttct
     8761 atttcgcacg gttcaacatt taaattcaca ttgccaccct catccttata tattgatgct
     8821 attaatttaa taattttatg ttgtttctta gcattttgta tttgctgttt tgtaacgtgc
     8881 tcgattcctt ttatatgtat atcagtaagt gttttctctg tttccattaa g
//
