LOCUS       NZ_NTWJ01000025        10261 bp    DNA     linear   CON 01-APR-2024
DEFINITION  Bacillus thuringiensis strain AFS009835
            AFS009835_104_C9_Contig25_140523, whole genome shotgun sequence.
ACCESSION   NZ_NTWJ01000025
VERSION     NZ_NTWJ01000025.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 118525)
  AUTHORS   Bleich,R.M., Kirk,G.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 118525)
  AUTHORS   Bleich,R.M., Kirk,G.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Assembly Cell v. 4.2.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 61.85x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002562205.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 01:49:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,978
            CDSs (total)                      :: 5,906
            Genes (coding)                    :: 5,721
            CDSs (with protein)               :: 5,721
            Genes (RNA)                       :: 72
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 82 of 185
            Pseudo Genes (incomplete)         :: 109 of 185
            Pseudo Genes (internal stop)      :: 57 of 185
            Pseudo Genes (multiple problems)  :: 52 of 185
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:13:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 88570
            Orig. end                         :: 98831
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NTWJ01000025.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10261
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10261
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10261
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5261](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5261
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            978..2339
                     /locus_tag="CN427_RS25625"
                     /old_locus_tag="CN427_25630"
     CDS             978..2339
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017763222.1"
                     /locus_tag="CN427_RS25625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_25630"
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_016122599.1"
                     /transl_table=11
                     /translation="MNGKRKIFTCISIVGIGLASFSNSSFAASVTDNSIQNSIPVVNQQ
                     VAAAKEMKPFPQQVNYAGVIKPNHVTQESLNASVRSYYDNWKKKYLKNDLSSLPGGYYV
                     KGEITGDADGFKPLGTSEGQGYGMIITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNL
                     MGWVVADSKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGTVNYLKEAQDMITKGIKAS
                     NVTNNNRLNLGDWDSKSSLDTRPSDWMMSHLRAFYEFTGDKTWLTVINNLYDVYTQFSN
                     KYSPNTGLISDFVVKNPPQPAPKDFLDESEYTNAYYYNASRVPLRIVMDYAMYGEKRSK
                     VISDKVSSWIQNKTNGNPSKIVDGYQLNGSNIGSYPTAVFVSPFIAASITSSNNQKWVN
                     SGWDWMKNKRESYFSDSYNLLTMLFITGNWWKPVPDDKKTQNLINDAIYEGYDN"
     gene            2502..3425
                     /locus_tag="CN427_RS25630"
                     /old_locus_tag="CN427_25635"
     CDS             2502..3425
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="CN427_RS25630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_25635"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_000416776.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATKPLTEEITTYYPEIHGAEGLGPIHVPENILSIPIYNF
                     GSIAAILIKYGKDLTIVDVGRSTSLAIAFNLWSDLMLNVKGIYFMGGVFLEVGNVTPLA
                     EANVYGDPIASKIVFHQAKNLLIFPLNVTNKAVLTPNVFNYIQANTKNPFHMIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVVAISGLVNPSFFEFVHRTVNVDTYGDTKGQTFADFRPS
                     SISDGAHIALEIDEQKFIQDFIKIML"
     gene            3502..3960
                     /locus_tag="CN427_RS25635"
                     /old_locus_tag="CN427_25640"
     CDS             3502..3960
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="CN427_RS25635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_25640"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_001102684.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRENWEEALKLQVKGNQLKFVPSVAVSLAKVYIKPDG
                     DNVEYIPFAIYDGDLMVGFVMHAVVIETSDMYWINGFIIDQSQQGNGYGKAALQESIDI
                     IKNTFKSCKEIRLTVHKDNISAKKLYERYGFISLGQEYDGEQVYRLFV"
     gene            4018..4338
                     /gene="cutA"
                     /locus_tag="CN427_RS25640"
                     /old_locus_tag="CN427_25645"
     CDS             4018..4338
                     /GO_process="GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="cutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470501.1"
                     /locus_tag="CN427_RS25640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_25645"
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="WP_000470501.1"
                     /transl_table=11
                     /translation="MFIPKEYIETLRDELNKIGACKTGEYDHCLSYSSVKGYWRPLEEA
                     SPFNGEIGQICEGQECKVEIKCKRELVKDALEVINDIHPYETPMIYIIPILNDYFEKLY
                     IK"
     gene            4355..4801
                     /locus_tag="CN427_RS25645"
                     /old_locus_tag="CN427_25650"
     CDS             4355..4801
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="CN427_RS25645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_25650"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_000836831.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAVMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LKRELREELGVNCSIQQFLGVIENQWQDKEVLHHEINHIFEVESQDLHTDLTPKSSESH
                     LAFHWIAYNKEALNHYEIMPMPLVKELLESKLNDELLNCWISNF"
     gene            5001..5261
                     /locus_tag="CN427_RS25650"
                     /old_locus_tag="CN427_25655"
     CDS             5001..5261
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071342.1"
                     /locus_tag="CN427_RS25650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_25655"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001071340.1"
                     /sec_met_domain="TIGR03601 (E-value: 1.3e-40, bitscore:
                     130.8, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQHELQTLNLNDYQTGNVVYWDQHQSQYPYYYIQDDARRCGG
                     CGGCGGRCGGCGGGRCGGCGGRCVGCAGCFSCFNCWNWWII"
     gene            5363..6379
                     /locus_tag="CN427_RS25655"
                     /old_locus_tag="CN427_25660"
     CDS             5363..6379
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="CN427_RS25655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_25660"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_000288887.1"
                     /transl_table=11
                     /translation="MYTYDKLISWVEDIKEKNHSSATALCIIKDNKIILEHYSGYHSNT
                     STSKKVTASSQFNVASARKSYLGLMIAYALYEGKINSIDDEAIKYFKDFDPVLLGKTTI
                     RHLVTHSHGLEETNDRTIFREFEPGQSWAYRDVNVRMMTRLIYQLYNKSFPELLKERVF
                     KPANFQETGWRVQRDENLVDVVNNPNEDAISEIGTVDDGTEKNLFVSAREFAQWGNLHL
                     NQGMIDDKQIVPKEVIKIATSLQSPTFINKELPQNGVFWFIQNEPAQLSELGERIPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSNGNRA"
     gene            6411..6554
                     /locus_tag="CN427_RS31445"
                     /old_locus_tag="CN427_25665"
     CDS             6411..6554
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="CN427_RS31445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_25665"
                     /product="hypothetical protein"
                     /protein_id="WP_000653680.1"
                     /transl_table=11
                     /translation="MKDKVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            6834..8042
                     /locus_tag="CN427_RS25665"
                     /old_locus_tag="CN427_25670"
     CDS             6834..8042
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="CN427_RS25665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_25670"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_072938623.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAAKLQKKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLIDNLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNAVTETLHNDYTPREMMRTKQVYLLFFMLFTS
                     CMGGLYLISMVKDIGVQLVGLSAATAANAVAMIAIFNTVGRIILGTLSDKIGRIKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGAILGGFQPTFIIIGVLSFISFIISILIRPPTAEKTKELKHLHR
                     KVA"
     gene            8293..8919
                     /locus_tag="CN427_RS25670"
                     /old_locus_tag="CN427_25675"
     CDS             8293..8919
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633406.1"
                     /locus_tag="CN427_RS25670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_25675"
                     /product="cadmium resistance transporter"
                     /protein_id="WP_000633410.1"
                     /transl_table=11
                     /translation="MITTIISSVVAFATTNIDDIFILLVLFSQVRTEVIRKEGRAVREK
                     ATRKKLYIVIGQYLGFSMIIFLSIIGSLSSFFIPVSWIGLLGLVPIYMGVKGLLSLRSY
                     KSNEVIDNVTVSLFKVASITLANGADNISIYIPMFASQTLETNIVTLVIFFSMIAIWCF
                     ISYKLIKAPILAKVLEENCHIIVPIVLIGLGMFILFRSGTIGLFS"
     gene            9169..9585
                     /locus_tag="CN427_RS25675"
                     /old_locus_tag="CN427_25680"
     CDS             9169..9585
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016104977.1"
                     /locus_tag="CN427_RS25675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_25680"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_097853357.1"
                     /transl_table=11
                     /translation="MKIVQQWVQEDSDYIRERVIEYNQKYLADEEKTPSEKVSFIVRNE
                     KEEIVGGVTAITFWHHVHVDFLWVSEEYRHEGYGSKLIKLIEEFAIEKGCRLINLDTFS
                     FQAPDFYKKHGYKVIGVSEDHPKGHNRYYLEKRL"
     gene            9805..10248
                     /locus_tag="CN427_RS25680"
                     /old_locus_tag="CN427_25685"
     CDS             9805..10248
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="CN427_RS25680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN427_25685"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_000757628.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDKTNEKILMVKNKGENGSYYTLPGGAVKLGETL
                     EEAVIREVKEETGLHINVNGICSISEAFFEERDHHAIFFNFLGEIIGGETYISRPKEIE
                     EITWMELHIAAPYLRIPEHLLDLLQKKETVPYFFNGTIVHQSS"
ORIGIN
        1 cagcaattcg tctggggctt gaatcttata cgcctgaaca ccttacttta ctacgcttat
       61 taatcgcctc atttatactt ttgttatttt catttatata caaactacga ctaccggatt
      121 taaaggatat cccagcaatt tttatatttg gtgctttagg gtttactatt tatcacatcg
      181 cattaaatta cggcgaaaaa acagtaaatg ctggatctgc aagtttaatt atttcagtca
      241 ccccaatagt gacggcaatt cttgcttctg tttttttgaa tgacaaaatg aaattaaatg
      301 gttggcttgg tggtgtaatt agttttgcag gaattgcctt aatttcattt agccagggcg
      361 atgctattca attgaatagc gggggattat ttatattatt agcggcgatt tcagaaagtc
      421 tatttttcgt tttccaaaca tcttacttaa aaaaatacgg cttcttacca tttaccatat
      481 atacaatttt gtctagtact gtatgtatgc ttattttctt acccggagtt tatcaagaaa
      541 tactagcagc tcctcttgaa gttaacttga gcgttatata tttaggtgtc tttccaacag
      601 tactcccgta tattgcattg gcctatatta tatctcgtgc tggtgcttct gaagcaacaa
      661 gttctctata tttaacgcca ataactgcgt gtttcattgc ttggatatgg ttaggagaag
      721 tgccaacttt agtttcgata attggcggag gaattactat acttggaatt gtgattgctc
      781 atataccggt attaagaaaa gaaaaacata ggaatttagc aaataatcaa tccgtataat
      841 gtaattcaat aataatttta tgatactttt ttatatagtt tctttcttat gttgataaat
      901 ataatatttt gtatttcagt taaatattgt atgttacatt ttatgtagta agcattttaa
      961 aaggagctga caacataatg aatggaaaaa gaaaaatttt cacatgtatt tctattgtag
     1021 gaatcggact agctagtttt tctaattcta gtttcgcagc aagtgtaacg gacaattcaa
     1081 tacaaaattc tattcccgta gttaatcaac aagtagctgc tgcaaaggaa atgaaaccat
     1141 ttccccagca agttaattat gcaggtgtta taaaaccgaa tcatgttaca caggaaagtt
     1201 taaatgcttc tgtaagaagt tactacgata attggaaaaa gaaatatttg aaaaatgatt
     1261 tatcttcttt acctggtggt tattacgtaa aaggagagat tacaggtgat gcggatgggt
     1321 ttaagccact tggaacttca gaaggtcaag ggtatgggat gataattaca gtattaatgg
     1381 ctggttatga ttcgaatgct caaaaaatct atgacggttt atttaaaaca gcaagaactt
     1441 ttaaaagctc tcaaaatcct aatttaatgg gatgggttgt cgcagatagt aaaaaagcac
     1501 aaggtcattt tgattctgct actgatgggg atttagatat tgcgtattct cttcttcttg
     1561 ctcataagca gtggggatct aatggaacag ttaattattt gaaagaagca caagacatga
     1621 ttacaaaagg tattaaagct agtaatgtta caaataataa ccgactaaat ttaggagatt
     1681 gggattctaa aagttcactt gatacgagac catctgattg gatgatgtca caccttagag
     1741 cattttatga atttacaggt gataaaactt ggcttactgt tattaataat ttgtacgatg
     1801 tttatacgca atttagtaat aagtactctc caaatacagg acttatttca gatttcgttg
     1861 taaaaaaccc accacaaccc gcacctaaag acttcttaga tgagtcagaa tatacaaatg
     1921 catattatta taatgctagt cgagtacctt taagaattgt aatggactat gcgatgtacg
     1981 gcgagaagcg aagtaaagtt atttctgata aagtctcttc atggattcaa aataaaacga
     2041 atggaaatcc ttctaaaatt gtggatggtt atcaattaaa tggatccaat attggtagtt
     2101 atccaactgc tgtatttgtt tcaccgttta ttgctgcaag tataacgagt agcaataatc
     2161 aaaagtgggt aaatagtggt tgggattgga tgaagaataa gagagaaagt tattttagtg
     2221 atagttataa tttattaact atgttattta ttacaggaaa ttggtggaag cctgtacctg
     2281 atgataaaaa aacacaaaat ctaataaatg atgcaattta tgaaggatac gataattaat
     2341 taaatttgag aatggttact gaagaaagga attagcattc gctagttcct ttttaatgtt
     2401 ttgtaaaaca ttatccattc taaaattcgc atagatatac ttgctttttt catatacata
     2461 gttattgatg actctatgtt tttatgaagg gagagtattt cttggaaaaa gtattatttt
     2521 tcggagaccc aggtattgat gactcatttg ccattatgta tggtttactg catcccgaaa
     2581 ttgaaattgt cggtattgta accggatacg gaaatgttga gcatatacat gctgcacata
     2641 atgcagcgta tattttacag ttagcaaata gacaagacat tcctgtcatt agtggtgcaa
     2701 caaaaccact tacagaagaa atcactacgt actatcctga aattcacgga gcagaaggat
     2761 tagggcccat ccatgttcca gaaaatatat tatctattcc aatatataat ttcggcagta
     2821 ttgctgcgat tcttataaag tacggaaaag atttaacgat tgttgatgta ggaagatcta
     2881 cctcgttagc aattgctttt aatttatgga gtgacttaat gctgaatgta aaaggaatct
     2941 atttcatggg tggtgtcttt ttagaagtag gaaacgttac gccattagcg gaggcaaacg
     3001 tgtatggaga tcctatagca agtaaaattg ttttccatca agcaaagaat ttattaatat
     3061 tccctttaaa tgtaacaaac aaagctgtgt taacaccaaa tgtatttaac tatattcaag
     3121 ctaatacaaa gaatccattt catatgataa tgaaacccat gtatgattac tatctatccg
     3181 cataccaaaa gcttaatcca tctatcgaag gacctttgct acatgatgtg gttgcaataa
     3241 gtggattagt aaatccaagt ttttttgaat ttgtgcatcg tacagtaaat gtagatacat
     3301 acggagatac gaaaggacaa acatttgctg attttcgccc tagctctata tctgatggag
     3361 cacatatagc actagaaata gatgaacaaa aattcattca agattttata aaaatcatgt
     3421 tataagttgc tgtcgaaaaa gggaattatt tcactacgtt gaatataaac atacatcctc
     3481 tatataaggg agcttacaat attgaatgtc cagttaaagg ttgttacgag agaaaattgg
     3541 gaagaggcat taaagctaca agttaaagga aatcagttga aatttgttcc atccgtagca
     3601 gtttcgcttg ctaaagtata tataaaacca gatggtgaca atgtagagta cataccattc
     3661 gctatatacg atggtgacct tatggttggt tttgttatgc atgctgttgt aatagagaca
     3721 tcggatatgt attggattaa cggatttatt attgatcaat cgcagcaagg taatggttat
     3781 ggaaaagcag cattacaaga aagtattgat ataataaaaa atacttttaa atcgtgtaaa
     3841 gaaataagat taaccgtaca taaagataat atctctgcaa agaaattata tgaacgatat
     3901 ggtttcatat cattagggca ggagtatgat ggtgagcaag tatatcgttt atttgtttaa
     3961 ctattaggaa gtaaaggtgt gaaatgaaca cttcaattta ctgaagcgaa aattgaagtg
     4021 ttcattccta aagaatatat tgaaacatta agggatgaat taaataaaat tggagcatgt
     4081 aaaacgggcg aatatgacca ttgtctttcc tatagttcag tgaaaggata ttggagacct
     4141 ttagaagagg catctccttt taatggagaa attggtcaaa tatgcgaagg acaagaatgt
     4201 aaagttgaaa taaagtgtaa acgagaactt gtaaaagatg ctcttgaagt tattaatgat
     4261 attcatccgt atgaaacacc tatgatttat attataccaa tactaaatga ctattttgag
     4321 aaactataca ttaaataact aggaggaata tcaattgaaa agtaaatttc atcatattgt
     4381 acgagcagtc atgataaaag atgaaaaatt attagttgct gaatatatcg gccatcatta
     4441 ttttttacct ggtggccatg ttgaaattgg agaatcagca gagaatgcat taaaaagaga
     4501 gttaagagaa gaacttggag taaactgtag tatacaacaa tttttaggcg tcatagaaaa
     4561 tcaatggcaa gataaagaag ttcttcatca tgaaattaac cacatttttg aagtagagtc
     4621 acaagattta catacggatt taacaccgaa atctagcgag tctcatttag catttcactg
     4681 gatagcttat aataaagaag ccttaaatca ttatgaaatc atgccaatgc ctttagtaaa
     4741 ggaattacta gaaagtaagt taaatgatga actcctaaat tgttggatta gtaattttta
     4801 aatcacttta tttatagtaa atacgtccat cgaatgaata taaagaaaat aggctaaaaa
     4861 acaaggtaca tcacctagtg taagttcata ttttgttgat gtacgcattt agtctatagt
     4921 cttgtttcta ctttgtttac aagaatttat taactttaat atttggacgg aatttacatt
     4981 tataaatgga ggggttcgta atgaatcagt ttcaacatga actacaaaca ttaaacctta
     5041 atgattatca aactggtaat gttgtgtatt gggatcaaca ccaaagtcaa tatccatact
     5101 actacattca agacgatgca cgtcgttgcg gcggatgcgg cggttgtggt ggacgctgtg
     5161 gtggttgtgg cggcggtaga tgtggtggtt gtggtggacg ttgcgtaggt tgcgctggct
     5221 gttttagttg ctttaattgc tggaactggt ggattattta agtatagaaa atccaagtta
     5281 ttttatatga aagaaaggtg taaaaactat tttgcacttt tctttcatgc tgtaattttg
     5341 atgtgtatta agggggattt atttgtatac atatgataag ttgatttctt gggtagagga
     5401 tataaaagaa aaaaatcata gttctgctac agcactttgt attataaaag ataataaaat
     5461 catactagag cattatagtg gttatcactc aaatacatct acaagcaaga aagtaacagc
     5521 atcttcacaa tttaacgttg cttctgctag aaaaagttat ttaggattaa tgatagcgta
     5581 tgcgctttac gaggggaaaa taaactctat cgatgatgaa gcgataaaat attttaaaga
     5641 ctttgatcct gtattgcttg gtaaaacgac gataagacat ttagtaacac attcgcacgg
     5701 attagaagaa acgaatgaca ggacaatttt tcgtgaattt gaaccgggac aatcatgggc
     5761 gtatagagat gttaatgtaa gaatgatgac acgtcttatt tatcagctat ataacaaaag
     5821 ttttcctgaa ttgttaaagg agcgtgtgtt taaacctgct aattttcaag aaacaggttg
     5881 gagagttcag cgagatgaaa atttagttga cgttgttaat aatccaaatg aagacgcaat
     5941 tagtgaaatt ggtacggtag atgacggtac tgaaaaaaat ttatttgtct cagctagaga
     6001 atttgcgcag tggggtaatc ttcatttaaa tcaaggtatg atagatgata aacaaattgt
     6061 tccaaaagaa gttataaaaa tcgctacgag tttgcagagc ccaacattta taaacaaaga
     6121 gctaccacaa aatggggtgt tttggttcat ccaaaatgaa cctgcacaat taagcgaact
     6181 tggtgaacgt attccaaaag ggtcgtatca aatattaggg attactggac cgactatttt
     6241 agtaatacct gaatataatg tagttgttgc aaaaatgtat aataaaagat ataactacgg
     6301 tggtgataat tacttatatt atttacgtga atttagtaat ttagtcgctg atacatttag
     6361 taacggtaat agggcataat tactcctata acaaaaagga gtgattgaaa atgaaagata
     6421 aagtgaacaa aaataaaaag caaaatataa aagatgtaaa tacagagcaa aatgcaattt
     6481 atagcgatcc taaagatgca gctaatatgc aaactgtacc gcagccgaaa gattttgatg
     6541 aaatcgaata ttaatttttc atccgactat atgatgaatg aaaaatacgt cttaagtctc
     6601 ataacattta agaaattatt tgataacata tatacataag catttgaaaa tgtcttatct
     6661 taacaaagct ttctttagaa acatctgata ttacctgcaa aagacacttc tttatgaagt
     6721 gtctttttgt gtatatgaac ttaattacca ttttccgtac agtatcttgc aatttatgat
     6781 ggatatacta actgtgaaaa ttcatgtcaa gataaaagaa ggggacagtt agtatgaaac
     6841 aaacttctat aaatccgtta ctaattgttc taggtacaat cattgttcaa attggccttg
     6901 gaacaattta tacatggagt ttatttaatc aaccccttgt aagtaagttt ggatggaatc
     6961 ttaattcagt agcgataact ttctccataa ctagtttttc tttatcattc tcaactttat
     7021 ttgcagccaa gttgcagaaa aaattaggac ttcgaaaact tattgctact gcaggaattg
     7081 ttttaggact cggcttaata cttagttcac aagtttcttc cttaccacta ttatatttat
     7141 tagctggtgt cgttgttggt tatgcggatg gaacagctta tattacatca ctatctaatt
     7201 taattaaatg gtttccaaat cggaaagggc ttatttcagg tatatctgta tcagcgtatg
     7261 gaatgggcag cttaatcttt agatatataa acggaaatct tatcgataac cttggtgtat
     7321 cacaagcatt cttatattgg ggtattatcg tgttactttt agtgttaatc ggatcgttct
     7381 tcttacgtga ggcaattgta agtaatgctg taactgaaac attacacaat gactatactc
     7441 cgcgtgaaat gatgcgaacg aaacaagtat atctgctatt ttttatgtta ttcacctcgt
     7501 gtatgggtgg tttgtattta atcagtatgg taaaagatat cggcgtacaa cttgttggac
     7561 ttagcgcagc aaccgctgca aacgccgttg ctatgattgc aatctttaat acagtaggta
     7621 gaatcattct tgggacgtta tcagataaaa tcgggcgaat caaaattgtc tctgcaacgt
     7681 ttattattat aggattgtca gtctttactt taagttttat tccgctaaat tacggaatct
     7741 attttgcatg tgtagcaagt gtcgcctttt gtttcggcgg taatataact atattcccag
     7801 ctattgtcgg agatttcttt ggattaaaaa accatagtac aaattatggg attgtctacc
     7861 aaggtttcgg atttggtgcg cttgcaggat cattcattgg agcgatactt ggaggatttc
     7921 aaccaacttt cattataatt ggtgttttaa gttttatttc cttcattatt tcaatattaa
     7981 tccgtccacc aactgcagag aagacaaagg aactaaaaca tctacatcgg aaagtggcgt
     8041 aatactaaaa agagtcctaa tatagggctc tttttagtac aaaacatttt ttgtgtagag
     8101 agaatagagt tatttaattt aaaatgaaat gagtagcaga tacttagaat attcatattg
     8161 ttttacgaag gaactaacat gttgctaaaa acatttagaa aattacagtt aaccaacttt
     8221 gtgatggaac gtgcacttca ttcatagacc attattttct tataagttta caatgaaagt
     8281 agttggcttt tattgattac aaccataatt tcttctgttg tggcattcgc tacaacaaat
     8341 attgatgaca tttttatact acttgtttta ttttcacaag taagaacaga agtaattagg
     8401 aaagaaggaa gagctgtccg cgaaaaagct acgagaaaaa aactttatat tgttattgga
     8461 caatatttag gatttagtat gattattttt ctaagtatca ttgggtcttt aagctctttt
     8521 ttcattcctg tttcgtggat tgggttatta ggattagtgc caatttatat gggggttaaa
     8581 ggactattgt cacttcgttc ttataagagt aatgaagtta tcgataacgt tactgtttca
     8641 ttatttaaag tagcttcaat tacattagct aatggagctg acaatatttc gatttatata
     8701 ccaatgtttg ctagtcaaac tcttgaaaca aatatcgtta cattagttat ctttttttcc
     8761 atgatagcga tatggtgttt tattagctac aaattgataa aagctcctat actagctaaa
     8821 gtacttgagg aaaactgtca tattattgtt ccaattgttc taattggttt aggaatgttc
     8881 attctttttc gtagtggtac aattggacta ttctcctaag gtgttgaaat aacggaagtt
     8941 ttcataaaaa agaattgaac aattttaata ttttttgagc aattatattc attgagatta
     9001 attagattct acacaacgta tataagtctt attacagatg aaatgtagta gggctttttt
     9061 tattttagtg aaatgaaaca attgtattag tcatctacga ctattattta gacaggagat
     9121 tagaattatg aagttgaata gctatcgaaa aggagtgtga cacttaaaat gaaaatagta
     9181 cagcagtggg tacaagaaga tagtgattac ataagagaga gagtgattga atacaatcaa
     9241 aaatatcttg cagatgaaga aaaaacccct tcagagaagg ttagtttcat agtaagaaat
     9301 gaaaaagaag aaatagtagg tggggtaaca gcaataactt tttggcacca tgtccacgtt
     9361 gactttctat gggtttctga agaatatagg catgaaggtt acggaagcaa gttaataaag
     9421 cttattgaag aatttgcaat tgaaaaaggg tgtaggttaa ttaatttaga tacttttagt
     9481 ttccaagctc ctgattttta taagaaacat ggatataaag taattggagt aagtgaagat
     9541 catccgaaag gacacaatcg atactattta gaaaaaaggt tataaagtat atagaattca
     9601 aaaatgaatc aattagagca taaagagcgt acattgaata atcaatgcac gctctgtttt
     9661 attttgtaat gaaggactat cgattaatta actttgctaa gagcagataa atgaaatact
     9721 caattaactt ttttatattt taaaacgaat aaatggtaga atgtaacaaa gatagaaaat
     9781 tcccattatt agaggtggac ttagatgaaa aaagtaaatg ttacatatgc acttttatac
     9841 gataaaacaa acgaaaagat cttaatggta aaaaacaaag gggaaaatgg ttcttattat
     9901 acattaccag gtggcgcagt taaattagga gaaacgttag aagaagcagt aattcgagaa
     9961 gtaaaagaag aaacagggtt acatataaat gtaaacggga tttgttctat cagcgaagcc
    10021 ttttttgagg aaagagatca tcatgcaatt ttctttaatt ttctaggcga aataatcggc
    10081 ggggagacat atatatcaag gccaaaagaa atcgaagaga ttacttggat ggaattacat
    10141 atagcagcac cgtatttacg cataccagaa catttactag atttattaca aaagaaagaa
    10201 acagtacctt atttttttaa cggaacaatc gttcatcaat cttcataaaa atattatctc
    10261 a
//
