LOCUS       NZ_CP022685            11299 bp    DNA     linear   CON 17-JAN-2024
DEFINITION  Streptomyces formicae strain KY5 chromosome, complete genome.
ACCESSION   NZ_CP022685
VERSION     NZ_CP022685.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces formicae
  ORGANISM  Streptomyces formicae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9611874)
  AUTHORS   Holmes,N.A., Devine,R., Qin,Z., Seipke,R.F., Wilkinson,B. and
            Hutchings,M.I.
  TITLE     Complete Genome Sequence of Streptomyces formicae KY5, the
            formicamycin producer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9611874)
  AUTHORS   Holmes,N.A., Devine,R., Qin,Z., Seipke,R.F., Wilkinson,B. and
            Hutchings,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2017) School of Biological Sciences, University of
            East Anglia, Norwich Research Park, Norwich, Norfolk NR4 7TJ, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.0; Newbler v. 2.3
            Assembly Name                     :: KY5 Genome
            Expected Final Version            :: yes
            Genome Coverage                   :: 56.0x
            Sequencing Technology             :: PacBio; 454; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/17/2024 10:10:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,274
            CDSs (total)                      :: 8,185
            Genes (coding)                    :: 8,082
            CDSs (with protein)               :: 8,082
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 39 of 103
            Pseudo Genes (incomplete)         :: 77 of 103
            Pseudo Genes (internal stop)      :: 11 of 103
            Pseudo Genes (multiple problems)  :: 22 of 103
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:13:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7219937
            Orig. end                         :: 7231236
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022685.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Matthew Hutchings,
            University of East Anglia, UK.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11299
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="31"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11299
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11299
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6299](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6299)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(112..132)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.24"
                     /tool="antismash"
     gene            complement(407..2008)
                     /locus_tag="KY5_RS31535"
                     /old_locus_tag="KY5_6208c"
     CDS             complement(407..2008)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (107-478). E-value:
                     3.4e-20. Score: 64.6. Matches aSDomain:
                     nrpspksdomains_KY5_RS31535_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505456.1"
                     /locus_tag="KY5_RS31535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_6208c"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_098245397.1"
                     /sec_met_domain="PF00561 (E-value: 5.1e-24, bitscore: 84.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAAARYGTAGSLVLTALVAAPAGSASGAPPSPETRGTAVAAQR
                     AEAAGIRWGTCAKAEGLPPSVRCGTVTVPLDYAHPDGKKIKLTVSRTKATKKAAGKAVA
                     RQGALVFNPGGPGGSGMYFPLVGVLPEWKRIAAAYDLVGYAPRGVGRSAPLTCQDPKQY
                     VKAPTQAPTHPDASYKRERVAQAKAYARGCGTRNPGLRHYTTLNNARDLDVLRAAVGER
                     KLTFMGASYGTYFGAVYATLFPSHVRRMVFDSAVNPEPSQIWYGNNLEQSMAFEGRWAD
                     FRAWVAKHDKVYHLGGTPDEVLRSYEKVRAELSHKPAGGKVGPGQLQAAFLKAGYYDDY
                     WPTRASALSAYLKGDPKPLIEQAAPEPKGAAEEENGNAVYTAVECNDAAWPTDFRVWDR
                     DNSDLARVAPFETWDNAWMNLPCAYWPAPRQQAVDVRTAKGALPPTLILAAERDAATPY
                     EGARELNRRLRDSVLVTEEGAGTHGIAAGPNKCVNGHLDAYLLEGRLPERHAECAPHRE
                     PKPLSGERAADLPKGV"
     aSDomain        complement(575..1687)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KY5_RS31535_Abhydrolase_1.1"
                     /evalue="3.40E-20"
                     /label="KY5_RS31535_Abhydrolase_1.1"
                     /locus_tag="KY5_RS31535"
                     /protein_end="478"
                     /protein_start="107"
                     /score="64.6"
                     /tool="antismash"
                     /translation="ALVFNPGGPGGSGMYFPLVGVLPEWKRIAAAYDLVGYAPRGVGRS
                     APLTCQDPKQYVKAPTQAPTHPDASYKRERVAQAKAYARGCGTRNPGLRHYTTLNNARD
                     LDVLRAAVGERKLTFMGASYGTYFGAVYATLFPSHVRRMVFDSAVNPEPSQIWYGNNLE
                     QSMAFEGRWADFRAWVAKHDKVYHLGGTPDEVLRSYEKVRAELSHKPAGGKVGPGQLQA
                     AFLKAGYYDDYWPTRASALSAYLKGDPKPLIEQAAPEPKGAAEEENGNAVYTAVECNDA
                     AWPTDFRVWDRDNSDLARVAPFETWDNAWMNLPCAYWPAPRQQAVDVRTAKGALPPTLI
                     LAAERDAATPYEGARELNRRLRDSVLVTEEG"
     gene            complement(2106..2879)
                     /locus_tag="KY5_RS31540"
                     /old_locus_tag="KY5_6209c"
     CDS             complement(2106..2879)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034043.1"
                     /locus_tag="KY5_RS31540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_6209c"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_098245398.1"
                     /transl_table=11
                     /translation="MFWLPLLLLAWAATGLSCGRLCLAASRAATHERAAPRGHELTLYE
                     TAFLSGGPSRVADVALVAMAGARRLLIAHTGWATVVDPVGRDEVERSVLGAIGPDGQSR
                     IAPIRKGAATADAVRALGERLVAAGLAVPESAGTGVAAAARQVRAATVAVLALGALAVC
                     MPTQGRAASGQVPVAIWFSLPLMLCVGCLIIARIEVHPYTRWASPAGQRLLGALPAARD
                     ELTTVAVRGVRALPDPGLRAAFGHRSRPDTAQRGH"
     misc_feature    2911..2926
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 20.66"
                     /tool="antismash"
     misc_feature    complement(2941..2956)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.68"
                     /tool="antismash"
     gene            complement(2997..3743)
                     /locus_tag="KY5_RS31545"
                     /old_locus_tag="KY5_6210c"
     CDS             complement(2997..3743)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007385411.1"
                     /locus_tag="KY5_RS31545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_6210c"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_098245399.1"
                     /transl_table=11
                     /translation="MRSRSINGTGLVVAGLVATLAALLFPLWSYEDRSGTTLDRLNAET
                     VSTDFGPLSALDREFITKVRLAGLWELPAGQQAEERGTTKAVRTAGEHLVEGHTFLDAR
                     VREVAARLSLELPSQPNPQQRGWLAELSAAHGQTYDEDFANILRRAHGKVFSVVAEVRA
                     STRNSLVRALADDANTTVLDHIKVLEKTGLVDFDGLARDAATGQAPATRSPAPPGPTAT
                     PSPAAPVTPSPTFSLPPAASRPKPDK"
     misc_feature    complement(3746..3762)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.35"
                     /tool="antismash"
     misc_feature    complement(3791..3802)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
     gene            complement(3890..4996)
                     /locus_tag="KY5_RS31550"
                     /old_locus_tag="KY5_6211c"
     CDS             complement(3890..4996)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="KY5_RS31550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_6211c"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_098245400.1"
                     /transl_table=11
                     /translation="MSIAGVVYTVVISLCAAELIRGLAAARRTWSPHRTRPPLDAEHGV
                     YVRGTLEAAFLSGGPARVADALIAGLHEDGRLVVAGPGVVGIPRPTARNAGEQAVIDAY
                     AAGPSGALHGLRTAVMRSLPVQETGEALAGRGLVVRPGRQDKWRRRAGVQVLLSVFGFV
                     VAGMLTVAQYAGDSAASGSADSVGDGIAMLLPAGIFGVVSGLICLSVSSKQLTLAGQYA
                     LREYVGSAGHLTGAAHLVATRGLAAAHPDLRAQLVAAARVRVRVPAVTAAGTYPAPYQG
                     TNAGSGNSGTPTWCGSGGGGGGGGFTCSGGAGSACSGGGGSSCGGGGGSSCGGGGGSSC
                     GGGGGSSCGGGGGGSSCGGGGSSCGGGS"
     gene            complement(5001..6299)
                     /locus_tag="KY5_RS31555"
                     /old_locus_tag="KY5_6212c"
     CDS             complement(5001..6299)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010034053.1"
                     /locus_tag="KY5_RS31555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_6212c"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_098245401.1"
                     /sec_met_domain="DUF692 (E-value: 1.9e-96, bitscore: 321.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRHLGTGIGWRPEIADAVEAMPGIDWVEAVAENVCPGHLPDSLVR
                     LRERGVTVVPHGVSLGLGGADRPDEGRLRALAERAEALGSPLVTEHIAFVRAGGPLTAT
                     QPLEAGHLLPVPRTRDALDVLCENVRIAQAALPVPLAVENIAALISWPCEEMTEGQFLY
                     ELVERTGVRLLIDVANLHTNHVNRGEDPAKALDELPVEAIAYVHVAGGFERDGVWHDSH
                     AHPVPDAVLAVLADLASRVSPPGVLLERDENFPAPAELERELTAIRETLTTAGAPAAAT
                     GAESTTADPAPASPDARQRTGLAQAALLSALVAGTPAPEGFDHARLRVQSHALAAKRAD
                     VVAKVAPELPGILGGDYRAAFVEYARSRPMTGGYRHDALTFAEHLLLAGRPEEAQARRE
                     LSDWWLERSGPAPLSLRPAARLRRATRRVLLGR"
     gene            complement(6380..7144)
                     /locus_tag="KY5_RS31560"
                     /old_locus_tag="KY5_6213c"
     CDS             complement(6380..7144)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643870.1"
                     /locus_tag="KY5_RS31560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_6213c"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_098245402.1"
                     /transl_table=11
                     /translation="MRRVNGTALIIAALVATLGAIAFPVWSYADRSGTGPANLAAGSVP
                     TRWGPLSAADRDLLVKVRLAGLWEIPAGQQAVERAPTEAVKEAGDHLIVGHTDLDRRVR
                     SAAAQLGVELPSQPTAQQRGWLQELTDASGETYQRKFANLLRASHGKVFAVIAQVRDST
                     RNSLVRQLATDSNQTVLDHITMLEATGLVDFDGIANGAATSTASPTGPPPPSGATPPAP
                     SPAGPSGDVSTTSRPSPLPPGRIETGRPEPQM"
     gene            complement(7200..7847)
                     /locus_tag="KY5_RS31565"
                     /old_locus_tag="KY5_6214c"
     CDS             complement(7200..7847)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153152.1"
                     /locus_tag="KY5_RS31565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_6214c"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_098245403.1"
                     /transl_table=11
                     /translation="MTGGTRLDGRVERGNQTRRLVLRRTVDVASVEGLDALSLGRIATE
                     LKLSKSGVFALFGSKEELQLATVRAAGRIYLDTVVEPALRTPPGVARVWRLCTAWLDYS
                     EQRVFPGGCFFYGVIAEFDAREGPVHDALVRANRDWSELMERSLAEAVDAAELHADTDL
                     PQLAFECIALMETANAHSVLHDESRAYRWASTAIAARLRAAATDPALVPAPH"
     gene            complement(7844..8605)
                     /locus_tag="KY5_RS31570"
                     /old_locus_tag="KY5_6215c"
     CDS             complement(7844..8605)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015579256.1"
                     /locus_tag="KY5_RS31570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_6215c"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_234362948.1"
                     /transl_table=11
                     /translation="MSSDKTHPEASQGPDAPSAADSPFRHENTSRDEAPQFVLPLVARI
                     ERAAPPARTDALETAARAVLVLLSDERSVGEGEWAQAVRDWQDARIRKVVRRARGAEWR
                     RAETLPGITVTGKSAEVRVFPPVPLDGWPKELVKLQVSGTELDDPEPPPGADSAHPVLW
                     MSPDVEMSAGKAMAQAGHGAQLAWWELSDADRTAWRDAGFPLSVRTPDAARWRELTASG
                     LPVVRDAGFTEIAPGSCTVVADHPALRTARA"
     gene            8685..9680
                     /locus_tag="KY5_RS31575"
                     /old_locus_tag="KY5_6216"
     CDS             8685..9680
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317912.1"
                     /locus_tag="KY5_RS31575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_6216"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_098245405.1"
                     /transl_table=11
                     /translation="MERDLAIRLDGVGRRYALRGPWVLRGVDLGIGAGTLVRIEGANGT
                     GKSTLLRLLAGIDAPSEGRREGRPRTAFVPERFPASLPFSAVGYLTHLGRVHGLARGAA
                     ERSAEQWLERFGAGGYARTPLAELSKGSSQKVAVAQALLADPELLVLDEAWTGLDAAAR
                     AELDRAVAERRAAGGAVVFVDHDPRRLLGATDETYAVVGAALNRRTESGTWGEGEGGGS
                     AAPPAGPTVVIEAQGPLGAELPPSVASAVTGIGEPTPEGVTRLSVPESRSDVVLKELLM
                     ARPPWHVVTVGGAGPTVSSASASASASDSASASASASVSSSLSVDGGEPR"
     gene            9677..10348
                     /locus_tag="KY5_RS31580"
                     /old_locus_tag="KY5_6217"
     CDS             9677..10348
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997023.1"
                     /locus_tag="KY5_RS31580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_6217"
                     /product="ABC transporter"
                     /protein_id="WP_098245406.1"
                     /transl_table=11
                     /translation="MNALLHYQLALLARSQRWLPPVILYAVFLAVGVQGGQPVLDSLAY
                     AAAALLPVAAWLVRICATNEPPAARACSAAAAGPGRAHLASLLAALLATVALGAFAVLV
                     VTAISDASSTDARHVVPRLPSAAAGLLAMLVSALIGTAVGALTNWPLLRSAGRAVPALL
                     LLALLALVTTGSPAKSVLTDLTAGSREGVVPVPVGALAGAGAVAVAALWAACALSSRRG
                     G"
     gene            complement(10383..10589)
                     /locus_tag="KY5_RS42305"
                     /old_locus_tag="KY5_6218c"
     CDS             complement(10383..10589)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564411.1"
                     /locus_tag="KY5_RS42305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_6218c"
                     /product="hypothetical protein"
                     /protein_id="WP_234363245.1"
                     /transl_table=11
                     /translation="MSMGTGTGTGAGTGAGTGTGEVVGTGSGEPLAECVLCRAPTEYPE
                     SHKGITLCPVCEWQEAQRTACSG"
ORIGIN
        1 ggcgcgcact gaagcacgcg cccggtcctt ccgctgcctg cactgcggtc tcgacgtccc
       61 gatgaccgca ccgggcacca gccatcgcaa tcactgtccg aactgcctgt ggagccgtca
      121 cctcgacgac accccgggcg accgggcggc cgactgcggt gcccggatgg aacccctcgc
      181 catctccgtc aagggagacg gcgaatgggt cctcgtccac cgctgcgtcc actgcggcgt
      241 cctgcacgcg aatcgcacgg cgggcgacga caacgcgttg ccgctgaccc gtcttgcggt
      301 gcggccactc gcacaggccc cgttcccctt ggagcggctc ggggccctgt aaggggcgcg
      361 gggaactgcg cgaccagcca cagcggacgc gcggccgccg acggggctag acccccttgg
      421 gaagatcggc ggcccgttct cctgacaagg gcttcggctc ccggtgcggt gcgcattccg
      481 cgtgccgttc cgggaggcgg ccctccagga gatacgcgtc caggtggccg ttgacgcact
      541 tgttggggcc cgcggcgatg ccgtgcgtgc ccgccccctc ctcggtcacc aggaccgagt
      601 cccgcagcct gcggttgagt tcccgcgcgc cctcgtacgg ggtcgccgcg tcccgctcgg
      661 cggccaggat cagcgtcggg ggcagcgcgc ccttcgccgt gcgcacgtcc accgcctgct
      721 gccggggcgc gggccagtag gcgcagggca ggttcatcca ggcgttgtcc caggtctcga
      781 acggcgccac gcgcgcgagg tcggagttgt cgcggtccca gacccggaag tccgtcggcc
      841 aggccgcgtc gttgcactcg acggcggtgt agacggcgtt gccgttctcc tcctccgcgg
      901 cgcccttcgg ctccggtgcc gcctgctcga tcagcggctt cggatcgccc ttcagatagg
      961 cggaaagggc cgaggcgcgc gtcggccagt agtcgtcgta gtacccggcc ttgaggaagg
     1021 cggcctggag ctgaccgggg ccgaccttgc cgccggcggg cttgtgggac agctccgcgc
     1081 ggaccttctc gtagctgcgc agcacctcgt cgggcgtccc gcccaggtgg tacaccttgt
     1141 cgtgcttggc cacccaggcg cggaagtccg cccagcgccc ctcgaacgcc atcgactgtt
     1201 cgaggttgtt gccgtaccag atctgcgagg gctcggggtt gaccgccgag tcgaacacca
     1261 tccgccgcac gtgcgagggg aagagcgtcg cgtacaccgc gccgaagtag gtgccgtacg
     1321 aagcgcccat gaacgtcagc ttccgctcgc cgacggcggc ccgcagcacg tccaggtcac
     1381 gggcgttgtt cagcgtcgtg tagtgccgca ggcccggatt gcgggtgccg cagccgcgcg
     1441 cgtacgcctt cgcctgcgcg acgcgctccc gcttgtacga ggcgtcgggg tgggtcggcg
     1501 cctgggtggg cgccttcacg tactgcttgg ggtcctggca ggtcagcggc gccgagcggc
     1561 ccaccccgcg cggggcgtag ccgaccaggt cgtacgccgc agcgatgcgc ttccactcgg
     1621 gcaacacgcc caccagcggg aagtacatgc ccgagccgcc ggggccgccc gggttgaaga
     1681 cgagcgcgcc ctggcgcgcg accgccttgc ccgccgcctt cttcgtggcc ttggtgcggc
     1741 tgacggtcag cttgatcttc ttgccgtcgg ggtgcgcgta gtcgaggggc accgtgaccg
     1801 tgccgcaccg gacggacggc gggaggccct cggccttcgc gcaggtgccc cagcggatcc
     1861 cggcggcctc cgcgcgctgg gcggcgacgg cggtgccgcg tgtctcgggc gacggcggag
     1921 cgccgctcgc ggaacccgcg ggggcggcga cgagggcggt caggaccaag gatccggcgg
     1981 ttccgtagcg ggcggctgct gctctcatcg cgcttcttac cccttttgtt cggcggcact
     2041 actcgaacaa aagggatact tcgtgcggca gttggcgaag taaagcaccg gcccgccgtg
     2101 tcggctcaat gaccccgttg cgccgtatca ggccgggacc ggtgcccgaa ggccgcccgc
     2161 aggccggggt cgggcagggc ccggacgccg cgtacggcga cggtggtcag ctcgtcgcgg
     2221 gccgcgggca gcgcgccgag gagccgctgc cccgcggggg aggcccaccg cgtgtacggg
     2281 tggacctcga tgcgggcgat gatgaggcac cccacgcaga gcatcagcgg cagcgagaac
     2341 cagatcgcca ccgggacctg ccccgaggcc gcccgcccct gggtcggcat gcacaccgcg
     2401 agggcgccga gcgcgagcac cgccaccgtc gccgcgcgca cctgccgcgc ggcggccgcg
     2461 acgcccgtgc ccgcgctctc cggcacagcg agtccggccg cgacgagccg ctcgccgagg
     2521 gcgcgcaccg cgtcggcggt ggccgcgccc ttgcgtatgg gcgctatccg cgactggccg
     2581 tcggggccga tcgcgccgag caccgaacgc tccacctcgt cgcggccgac cgggtcgacg
     2641 acggtcgccc acccggtgtg cgcgatcagc agccgacgcg cccccgccat cgcgacgagc
     2701 gccacatcgg cgacccgcga ggggccgccg gagaggaagg ccgtctcgta gagggtcagc
     2761 tcgtggccgc ggggcgcggc cctctcgtgg gtggcggcgc ggctcgcggc gaggcagagc
     2821 ctgccgcagg agaggccggt cgcggcccag gcgaggagga gaaggggcag ccagaacatg
     2881 gcggatttct atgcgaaggc gctgcgcggc gccacggtgt tttcactatg tggacagcgg
     2941 gtgacgggtg ggtaacgttc cgtttttccg tcgagggggg cgggacgggg agcgggctac
     3001 ttgtccggct tcgggcgcga ggccgccggg gggagcgaga acgtcggtga cggggtgacc
     3061 ggtgcggcgg gggacggtgt ggcggtgggg cccggtgggg cgggggagcg ggtcgcgggg
     3121 gcctggcccg tggcggcgtc gcgcgcgagt ccgtcgaagt cgacgaggcc ggtcttctcc
     3181 aggaccttga tgtggtcgag gacggtggtg ttggcgtcgt cggcgagggc gcggaccagc
     3241 gagttgcggg tgctcgcgcg gacctcggcc accaccgaga agaccttgcc gtgggcgcgg
     3301 cgcaggatgt tcgcgaagtc ctcgtcgtac gtctggccgt gcgcggcgct caactcggcg
     3361 agccagccgc gctgttgggg attgggctgg ctcggcagtt cgaggctgag ccgggccgcg
     3421 acctcgcgta cgcgcgcgtc gaggaaggtg tggccctcga cgaggtgctc acccgccgtg
     3481 cgcacggcct tggtggtgcc gcgctcctcg gcctgctgtc cggcagggag ttcccacagg
     3541 cccgcgaggc gcaccttggt gatgaactcc cggtccaggg cggacagcgg cccgaagtcg
     3601 gtcgacacgg tctccgcgtt gagcctgtcg agggtggtgc ccgagcggtc ctcgtacgac
     3661 cagaggggga agagcagggc agccagggtc gcgacgagcc ccgcgacgac gagaccggtg
     3721 ccgttgatgg atcgggatcg catggtgcct cctgttgcgg caccgcacgt tagtgcgcgt
     3781 ggggtggggt gcgttccctt acgcccagga gtacgtcaac tgccgtacgg cggcgaccgg
     3841 tcggcggtcc cggcgtgcgc cctcggaaga ggggaggccg gactgctcat catgacccgc
     3901 cgccgcagga ggatccgccc ccaccgcagg aggatccgcc tcctccgccg ccgcaggagg
     3961 atccgccgcc tccgccacag gaggagccgc cacctccgcc gcacgacgag ccgccgcccc
     4021 cgccgcagga cgagccccca ccaccggaac acgccgagcc cgcgccgccg gagcaggtga
     4081 acccgccgcc cccgccaccg ccgcccgacc cgcaccacgt gggcgtcccg ctgttgccgc
     4141 tccccgcatt cgtgccctgg tacggagcgg ggtacgtccc cgccgcggtc accgcgggga
     4201 cccggacccg tacgcgggcc gcggcgacca gctgggcccg cagatcgggg tgggccgcgg
     4261 ccaggccgcg ggtcgccacc aggtgcgcgg cgcccgtgag atggcccgcc gagccgacgt
     4321 actcgcgcag cgcgtactgc ccggcgagcg tgagctgctt cgagctgacg gacaggcaga
     4381 tcaggccgct cacgacgccg aagatcccgg cgggcagcag catcgcgata ccgtcgccca
     4441 ccgagtccgc cgacccgctc gccgcgctgt caccggcgta ctgcgccacc gtcagcatgc
     4501 ccgccaccac gaacccgaag acgctcagga gcacctgtac accggcccgg cgccgccact
     4561 tgtcctgccg cccaggacgc acgacgaggc cgcgccccgc gagcgcctcg cccgtctcct
     4621 gcaccggcag gctccgcatg acggccgtcc gcagcccgtg cagcgcgccg ctcggccccg
     4681 cggcgtacgc gtcgatcacc gcctgctcgc ccgcattgcg cgccgtcggc cggggaatcc
     4741 cgacgacgcc ggggcccgcc acgaccagac ggccgtcctc gtgcagaccc gcgatcagcg
     4801 cgtcggccac gcgcgccggg ccgccggaga ggaacgcggc ctccagggtg ccgcgcacgt
     4861 agacgccgtg ctcggcatcg agcggcggcc gcgtccggtg cggcgaccag gtgcgccgag
     4921 cagcggccag gccccggatc agttccgcgg cgcacagcga gatgaccacc gtgtacacca
     4981 cacccgcgat gctcatgccg tcaccgcccg aggagtacgc gccgggtggc ccgtcgcagc
     5041 cgtgcggcgg gccgcaggga cagcggcgcg gggcccgaac gctccagcca ccagtcgctc
     5101 aactcccggc gcgcctgcgc ctcttcgggc cgccccgcga gcagcaggtg ctcggcgaag
     5161 gtcagcgcgt cgtgccggta gccaccggtc atcggccggg acctcgcgta ctcgacgaac
     5221 gccgcgcggt aatccccgcc gaggatcccc ggcagctcgg gcgccacctt cgccacgacg
     5281 tcggcccgct tggccgcgag cgcgtggctc tgcacccgca gccgcgcgtg gtcgaacccc
     5341 tcgggcgcgg gcgtccccgc cacgagcgcg gagagcagcg cggcctgcgc gagcccggtg
     5401 cgctggcgcg cgtccgggga ggcgggcgcc gggtcggcgg tggtggactc cgcgccggtg
     5461 gcggcggccg gtgcgcccgc ggtcgtgagc gtctcccgga tcgcggtcag ttcgcgctcc
     5521 agctcggcgg gtgccggaaa gttctcgtcg cgctccagga ggacaccggg cgggctcacc
     5581 cgggacgcga ggtcggcgag cacggcgagc acggcatcgg gcaccgggtg cgcgtggctg
     5641 tcgtgccaga cgccgtcccg ctcgaagccg cccgcgacgt ggacgtacgc gatggcctcg
     5701 accggcagtt cgtcgagggc cttggccggg tcctcgccgc ggttgacgtg gttggtgtgg
     5761 aggttggcga cgtcgatgag cagccgtacg cccgtgcgct cgacgagctc gtacaggaac
     5821 tgcccctccg tcatctcctc gcagggccag gagatcagcg ccgcgatgtt ctccacggcg
     5881 agcggcacgg gcagcgcggc ctgcgcgatg cggacgttct cgcacagcac gtcgagcgcg
     5941 tcacgggtgc gcggcaccgg cagcaggtgc ccggcctcga gcggctgcgt cgcggtgagc
     6001 ggaccaccgg cccgtacgaa cgcgatgtgc tcggtgacga gcggcgagcc gagcgcctcg
     6061 gcgcgctcgg ccagcgcccg cagccttccc tcgtcggggc ggtcggcgcc gccgagcccc
     6121 agcgagacgc cgtgcggcac cacggtcacc ccgcgctcgc gcagccgcac cagcgaatcg
     6181 ggcaggtgcc ccgggcagac gttctccgcc acggcctcca cccagtcgat gccgggcatc
     6241 gcctccacgg cgtccgcgat ctcgggacgc cagccgattc cggtgcccag atgcctcatg
     6301 tcgctccccc ttcgtccccc gttgccagga ccatggcccc gaccgtgcgg atcgaacccc
     6361 ggggaggaga cgttcagagc tacatttgag gttccggccg cccggtctcg atccggccgg
     6421 gaggcagggg cgaggggcgt gacgtggtgg acacgtcgcc gctcgggccc gcgggggagg
     6481 gggcgggagg cgtcgcgccg gacggcgggg gaggcccggt ggggctcgcc gtggaggtgg
     6541 ccgcgccgtt cgcgatcccg tcgaagtcga cgagcccggt cgcctccagc atcgtgatgt
     6601 ggtcgaggac ggtctggttg gagtccgtgg cgagctggcg gaccagggag ttgcgggtgc
     6661 tgtcgcggac ctgggcgatg acggcgaaga ccttcccgtg cgaggcccgc agcaggttcg
     6721 cgaacttccg ctggtacgtc tcgccgctcg cgtccgtcag ctcctggagc cagccgcgct
     6781 gctgtgcggt gggctggctc gggagttcga caccgagctg cgccgcggcc gaccgcacgc
     6841 gccggtcgag atcggtgtgg ccgacgatga ggtggtcccc cgcctccttg accgcctcgg
     6901 tcggcgcgcg ctcgacggcc tgctgtccgg cggggatctc ccacagaccg gcgagccgca
     6961 ccttcacgag caggtccctg tcggccgccg acagcggacc ccaacgggtg ggcacggacc
     7021 ccgcggccag gttggcgggc cccgtgcccg agcggtcggc gtaggaccac acggggaacg
     7081 cgatcgcccc cagcgtggcc acgagtgccg cgatgatcag agccgtgccg ttgacgcgtc
     7141 gcaaggtgcc tcccgaatcg ccgtggtagc gggagatacg gaagggacag gcgatgcgtt
     7201 cagtgcggcg cgggcaccag tgccggatcc gtcgccgccg cgcgcagccg cgcggcgatg
     7261 gcggtgctcg cccagcggta ggcccgcgac tcgtcgtgca gcaccgagtg cgcgttcgcc
     7321 gtctccatga gggcgatgca ctcgaaggcg agctggggga ggtccgtgtc cgcatgcagt
     7381 tcggcggcgt cgacggcctc ggcgagcgag cgctccatca gctcgctcca gtcgcggttg
     7441 gcgcggacga gcgcgtcgtg caccgggccc tcgcgggcgt cgaactcggc gatcaccccg
     7501 tagaagaagc agccgccggg gaagacgcgc tgctcggagt agtcgagcca cgccgtgcag
     7561 agccgccaca cgcgggcgac gccgggcggg gtccgcaggg cgggttccac caccgtgtcc
     7621 agatagatgc gcccggcggc ccgcacggtc gccagctgca actcctcctt ggagccgaag
     7681 agcgcgaaca cgccgctctt gctcagcttc agctcggtgg cgatccggcc gagcgacagc
     7741 gcgtcgagcc cctccaccga agcgacgtcg accgtgcgcc gcagcaccag gcggcgggtc
     7801 tggttgccgc gctcgacccg gccgtcgagc cgggtgccgc ccgtcacgcc cgtgccgtac
     7861 gcagggcggg gtggtcggcg accaccgtgc aggaaccggg ggcgatctcc gtgaagcccg
     7921 cgtcgcgcac caccggcagc ccgctcgcgg tgagttcgcg ccagcgcgcc gcgtccggcg
     7981 tgcgcacgga cagcgggaag cccgcgtcgc gccacgcggt ccggtccgcg tccgacagct
     8041 cccaccaggc cagctgggcg ccgtgacccg cctgggccat cgccttgccc gccgacatct
     8101 cgacgtcggg gctcatccag agcacggggt gcgcgctgtc cgcgccgggc ggcggctccg
     8161 ggtcgtcgag ctcggtgccc gagacctgga gcttgaccag ctccttgggc cagccgtcga
     8221 gcggcaccgg cgggaagacg cggacctccg ccgacttgcc cgtgaccgtg atgccgggca
     8281 gcgtctccgc ccgccgccac tcggcgcctc gggcgcgcct gaccaccttg cggatccggg
     8341 cgtcctgcca gtcgcgcacg gcctgcgccc actcgccttc gccgacggaa cgctcgtcgc
     8401 tcagcagcac gaggacggcg cgggccgcgg tctccagggc gtccgtgcgg gccgggggag
     8461 cggcgcgttc gatgcgggcg acgaggggca gcacgaactg cggggcctcg tcgcgcgagg
     8521 tgttctcgtg ccggaagggg ctgtccgccg cgctcggggc gtccgggccc tgcgacgctt
     8581 cggggtgggt cttgtcgctg ctcacgcgtc cagtctgcca ggcggaagat cggccgtacc
     8641 tgggattact cctcgcgcga aggggcgtgc gcgaggatga ccgcatggaa cgtgatctcg
     8701 cgatacggct cgacggggtg ggcaggcgct acgccctgcg cggtccctgg gtgctgcgcg
     8761 gcgtggacct cggcatcggc gcgggcaccc tggtgcggat cgagggcgcc aacggcacgg
     8821 ggaagtcgac cctgttgaga ctcctcgcgg gcatcgacgc accctcggag gggcgtcggg
     8881 aagggcgccc gcgcacggcg ttcgtccccg aacgcttccc cgcgtcgctg ccgttcagcg
     8941 cggtgggcta cctgacgcac ctgggccgcg tgcacggcct cgcgcggggc gccgctgagc
     9001 ggagcgccga acagtggctg gagcgtttcg gcgcgggcgg ttacgcgcgt acgccgctgg
     9061 cggagctgtc caagggcagc agccagaagg tcgccgtggc gcaggccctg ctcgccgacc
     9121 ccgaactgct cgtgctcgac gaggcgtgga cgggcctgga cgcggcggcc cgcgccgaac
     9181 tcgaccgcgc cgtggccgaa cggcgggcgg ccgggggtgc ggtggtcttc gtcgaccacg
     9241 atccgcggcg gctcctcggg gcgacggacg agacgtacgc ggtggtgggc gcggcgctca
     9301 accgccgtac ggagagcggt acttggggtg agggggaagg cggcggcagc gcggcgccgc
     9361 ccgcaggacc caccgtcgtc atcgaggcgc aggggccgct gggcgcggag ctgccgccgt
     9421 ccgtcgcgtc ggcggtgacg ggtatcggcg agccgactcc ggagggagtg acgcggctct
     9481 cggtgccgga gagccgttcc gacgtcgtac tgaaggaact gctcatggcg cggccgccct
     9541 ggcacgtggt gacggtgggc ggcgcggggc cgacggtgtc gtcggcgtcg gcgtcggcgt
     9601 cggcgtcgga ttcggcatcg gcatcggcgt cggcttcggt gtcgtcgtcc ctgtcggtgg
     9661 acgggggtga gcctcgatga acgcgctgct ccactaccag ctcgcgctgc tcgcgcggtc
     9721 ccagcggtgg ctgccgcccg tgatcctcta cgcggtcttc ctcgccgtcg gcgtgcaggg
     9781 cggccagccc gtcctggact cgctcgcgta cgcggccgcc gccctgctgc cggtggccgc
     9841 ctggctggta cggatctgtg ccaccaacga gccgcccgcg gcgcgcgctt gctccgccgc
     9901 cgcggcgggt cccggccgcg cgcacctcgc ctcgctgctg gccgcgctgc tcgcgacggt
     9961 ggcgctcggc gcgttcgcgg tcctggtggt gacggccatc agcgacgcgt cgagcacgga
    10021 cgcgcggcac gtggtccccc ggctgccctc ggccgcggcg ggtctcctcg cgatgctggt
    10081 cagcgcgctc atcggcacgg cggtcggcgc gctcaccaac tggccgctgc tgcgctcggc
    10141 ggggcgcgcc gtccccgcgc tgctgcttct ggcgctcctc gccctggtca ccacgggttc
    10201 gcccgcgaag tccgtcctga cggatctgac ggcgggttcg cgggaggggg tggtaccggt
    10261 accggtgggc gcgctcgcgg gggcgggtgc cgtcgcggtg gcggcgctgt gggcggcgtg
    10321 cgcgctcagt tcgcggcggg gcgggtaggg gcgcgggttc cttgacggcc tgcccgggct
    10381 cctcagcccg agcaggcagt gcgctgggct tcctgccact cgcagacggg gcagagggtg
    10441 atgcccttgt gggactccgg gtactcggtg ggcgcgcggc acaggacgca ctcggcgagg
    10501 ggctcgccgc ttcccgtacc cacgacttcg ccggtacccg tacctgcacc tgtacccgca
    10561 cccgtacctg tacccgtacc catgctcatg aagtgctcat cgcccatgcg gcccagccta
    10621 gctccgggcc tccacgggcc cggcggtcgc ggcggcgccg gtcgcccccg ccgcgccgcc
    10681 cgccgcgccg atctgctccg acacccgcac gaagcggaat ccgcgctcgc gcagcttcgg
    10741 gacgatcgtc cgcagggcgc gctcggtcgc gggggcggcg ctgcgcgtgc agtgcatgac
    10801 gaccaccgaa ccgggccgca ccccgtcgag cacctgccgg gcgaccgcgc ccgagtccgt
    10861 ggcgaaggcg tcgccgctca ccacgtccca ctgcacggcg gtgacgccgg gcgcgagcgc
    10921 gcgcagggct tgacggtcgt agcagccgcc gggaaaacgg aagtacggca ccgcgcgctc
    10981 gacgcccgcc ttgcggaagg cgtcgaaggc gcgctccatg tccgtgcgca tgcggggcgc
    11041 ggggacggtc ggcaggccgt agcagctgcc ggtgaaggcg tgatggctgt acgagtggtt
    11101 ggcgacctcg aagagcgggt ccttgccgat gtccttggcc tgtgccgggt actcctcggc
    11161 ccagcgtccc gtcatgaaga aggtcgccgg cacgtcgagc cgtcgcaggg tggcgacgag
    11221 cgcggggttg tcgaaccgct ccccggccgc cgcgcggggc ccctggtcgg cggtcatgtc
    11281 ggcgtcgaag gtgagcgcg
//
