LOCUS       NZ_CP022685            10306 bp    DNA     linear   CON 17-JAN-2024
DEFINITION  Streptomyces formicae strain KY5 chromosome, complete genome.
ACCESSION   NZ_CP022685
VERSION     NZ_CP022685.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces formicae
  ORGANISM  Streptomyces formicae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9611874)
  AUTHORS   Holmes,N.A., Devine,R., Qin,Z., Seipke,R.F., Wilkinson,B. and
            Hutchings,M.I.
  TITLE     Complete Genome Sequence of Streptomyces formicae KY5, the
            formicamycin producer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9611874)
  AUTHORS   Holmes,N.A., Devine,R., Qin,Z., Seipke,R.F., Wilkinson,B. and
            Hutchings,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2017) School of Biological Sciences, University of
            East Anglia, Norwich Research Park, Norwich, Norfolk NR4 7TJ, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.0; Newbler v. 2.3
            Assembly Name                     :: KY5 Genome
            Expected Final Version            :: yes
            Genome Coverage                   :: 56.0x
            Sequencing Technology             :: PacBio; 454; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/17/2024 10:10:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,274
            CDSs (total)                      :: 8,185
            Genes (coding)                    :: 8,082
            CDSs (with protein)               :: 8,082
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 39 of 103
            Pseudo Genes (incomplete)         :: 77 of 103
            Pseudo Genes (internal stop)      :: 11 of 103
            Pseudo Genes (multiple problems)  :: 22 of 103
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:13:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3356799
            Orig. end                         :: 3367105
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022685.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Matthew Hutchings,
            University of East Anglia, UK.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10306
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="20"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10306
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10306
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5306](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5306)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    516..530
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.11"
                     /tool="antismash"
     misc_feature    522..538
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 19.14"
                     /tool="antismash"
     gene            554..1051
                     /locus_tag="KY5_RS14315"
                     /old_locus_tag="KY5_2839"
     CDS             554..1051
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454773.1"
                     /locus_tag="KY5_RS14315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_2839"
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="WP_098242611.1"
                     /transl_table=11
                     /translation="MADGTGGIEDLGGVQGLAGLDFASVTPEEFAQIVKGLSGKEIAEL
                     ARDVELRGRVLAEVFTRMERQFRPDAAGSLKALIRWKITGSGDNEEAVYETDIADGTCT
                     VRKGRSDADPRVTLILADAEFLKLVSGNASPVTMFMTRKVKIAGDVALASGLTRYFDIP
                     KA"
     gene            1098..2813
                     /locus_tag="KY5_RS14320"
                     /old_locus_tag="KY5_2840"
     CDS             1098..2813
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KY5_RS14320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KY5_2840"
                     /product="hypothetical protein"
                     /protein_id="WP_098242612.1"
                     /transl_table=11
                     /translation="MTSTHSPARGRPPEAVVGLLPTARGGRWDRWRLPLALAPALVILV
                     AGFQRRWMSDDGHIYVRTVRQILAGNGPVFNAGERAESSTGTLWQWLLVAAGATGADIA
                     TAAMYGGLLLTAAGFALAALGALRLHGAERRVGALLPCGALVLLALPPVWDFATSGLET
                     GLATCWLAGAWLALVAGPRAWPTCVVLGLGPLVRPDLALVSLVFLAAQWLMTRPSWRRA
                     LAGAAAASALPVAYEVFRMGYYGHLMPLPGVTKEASRSLWTRGLAYLADFAGPYLLWVP
                     LLFLAAVALPSGRVRHSVPLVPALAPVVAGLLCWVYVIKVGGDFMHGRMFLPGLFLMLL
                     PVFLVPLTRAWTIAALGVGIWALACASVLRIPYEGRIGPSGVADERGVYVRQNAAPHPL
                     HHDFAGFPGNRAYARLVHDAADGGAPVLLLGRTPADAGAPGVTGIYNTLGFSGTVVPLR
                     GAALDPIGLAYPLAAHSEGINGRVGHDKRLPDEWIYADRGGPGAPPAGLDPARVDAARR
                     ALRCGPFAELRAATREPLTAGRFWRNLTGAVERTSFRFPDDPVRAERAACGGTS"
     gene            2948..3166
                     /locus_tag="KY5_RS41775"
                     /old_locus_tag="KY5_2841"
     CDS             2948..3166
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="KY5_RS41775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="KY5_2841"
                     /product="hypothetical protein"
                     /protein_id="WP_098242613.1"
                     /transl_table=11
                     /translation="MKRTARALTFVALTAALTVPTVGIASATAPVAAPTTTKSAPSHDW
                     DDDWHHYHHHYYPGVGVGLGVGLGILL"
     gene            complement(3236..3517)
                     /locus_tag="KY5_RS14330"
                     /old_locus_tag="KY5_2842c"
     CDS             complement(3236..3517)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020117194.1"
                     /locus_tag="KY5_RS14330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_2842c"
                     /product="hypothetical protein"
                     /protein_id="WP_098242614.1"
                     /transl_table=11
                     /translation="MTSPRRLIAAVSLAAGAAALLAPTAHASDRADATGAPSVLSAIDD
                     LGTTAIPDDHKDELPTVASQLSGLQRLNDLHQLTDLVAPVTNAVPAVQ"
     misc_feature    3546..3566
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.78"
                     /tool="antismash"
     misc_feature    3562..3575
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.37"
                     /tool="antismash"
     misc_feature    3598..3613
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.89"
                     /tool="antismash"
     misc_feature    complement(3638..3654)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.95"
                     /tool="antismash"
     gene            3737..4003
                     /locus_tag="KY5_RS14335"
                     /old_locus_tag="KY5_2843"
     CDS             3737..4003
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018568416.1"
                     /locus_tag="KY5_RS14335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_2843"
                     /product="chaplin"
                     /protein_id="WP_098242615.1"
                     /transl_table=11
                     /translation="MSRIAKAAAVTLGTGAVVLGGAGLAMADAGAHGEAVGSPGVISGN
                     LLQIPVNIPVNVCGNTVNGIGALNPAFGNQCANTGGDDAGYGG"
     gene            complement(4097..4969)
                     /locus_tag="KY5_RS14340"
                     /old_locus_tag="KY5_2844c"
     CDS             complement(4097..4969)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116480.1"
                     /locus_tag="KY5_RS14340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_2844c"
                     /product="tyrosinase family protein"
                     /protein_id="WP_098247247.1"
                     /transl_table=11
                     /translation="MAHTRKNQSSLSRAERRRFVDAVLKLKRSGQYDEFVRTHIDYYVS
                     DGERSLRVAHMTPSFFPWHRRFLLEFERALQRLDAGVTVPYWDWTKDRTPAVSLWGDDL
                     MGGNGRPSDGQVTTGPFAYGSGHWTVKVGVTDAEYLTRNLGRAQDPIDLPTARDVAWAM
                     EDSAYDAEPWDSTSEGGFRNRLEGWTSASGTERWRNHNRVHRWVGGHMLGGASVNDPVF
                     WLHHAYVDLLWDRWQRRHPDSAPYLPERPPRLGSAQYRRIVARHEPMPPWNVKPDALLE
                     HKKLYRYGS"
     misc_feature    complement(4745..4747)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5001..5306)
                     /locus_tag="KY5_RS14345"
                     /old_locus_tag="KY5_2845c"
     CDS             complement(5001..5306)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672801.1"
                     /locus_tag="KY5_RS14345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_2845c"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_267894320.1"
                     /sec_met_domain="melC (E-value: 3.5e-37, bitscore: 126.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFRTAQTRSAPDLAGPAGDLLFDEMYRGRRIVGQPSAGLDADAAG
                     DWNVTVDGRPLGLMRRADGSYLSMVDHYESHATPLSATRAAVDALGSHQTLSGRHH"
     gene            complement(5468..5980)
                     /locus_tag="KY5_RS14350"
                     /old_locus_tag="KY5_2846c"
     CDS             complement(5468..5980)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358348.1"
                     /locus_tag="KY5_RS14350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_2846c"
                     /product="hypothetical protein"
                     /protein_id="WP_098242617.1"
                     /transl_table=11
                     /translation="MKKSTRGALAVALACAAAAGAGAGTAAQAAEQPSVTVPLNGLEYA
                     LDMEAPELTTGVPVPLPGEGLDGPRYAQDSLLPERAVPRFPVSGVLPDTQLSAPLTDML
                     GEDTVESVGAEARGSSVEAVTPSPNLDLPLTAPQPELWGLPAPKLPNAGIEAPELRAQP
                     AANLGLA"
     gene            complement(6117..6803)
                     /locus_tag="KY5_RS14355"
                     /old_locus_tag="KY5_2847c"
     CDS             complement(6117..6803)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644657.1"
                     /locus_tag="KY5_RS14355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_2847c"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_098242618.1"
                     /transl_table=11
                     /translation="MRATSAPGRVPPQAVGEPDPVAPGEAAAPAPGPGWAADSGASRGL
                     GVLLVVTGALGLLAAWVITLDKFTLLEDPDFIPGCSLNPVVACGNIMKSEQAAAFGFPN
                     PLLGLATYPVVLCVGMSLLAGARFPRGYWLTLNAGTAFGVGFCTWLQFQSLYRINSLCL
                     WCCLAWAATLLMFWYVTSYNVSRGFLPVPAGIRVVLTEFSWAAPVLHMGVIGMLILTRW
                     WDFWIS"
     gene            complement(6803..7306)
                     /locus_tag="KY5_RS14360"
                     /old_locus_tag="KY5_2848c"
     CDS             complement(6803..7306)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008746418.1"
                     /locus_tag="KY5_RS14360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_2848c"
                     /product="DUF5949 family protein"
                     /protein_id="WP_098242619.1"
                     /transl_table=11
                     /translation="MTSTTSEQRPFHAADLGTLAVIAWSGEHPEDQKDMPFLLAYSLGD
                     TEGGPESTAEALTTMLHNAGLPIGGPLVDGARVPSMPVSLLVEADQAVVNMPQVNAQCP
                     VPPEWLAAVQQRGHAYFMATTRAWPEAKPGEKVSEELLSEFAGDEETLLNAAHCLLPAR
                     SLRS"
     gene            complement(7552..7803)
                     /locus_tag="KY5_RS14365"
                     /old_locus_tag="KY5_2849c"
     CDS             complement(7552..7803)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018529519.1"
                     /locus_tag="KY5_RS14365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_2849c"
                     /product="chaplin"
                     /protein_id="WP_098242620.1"
                     /transl_table=11
                     /translation="MKNAKKAALVLAAAGLAAGAASGSAFATDGDGASAQGAAVESPGV
                     ISGNLGQVPVDVPVNVAGNTGTLIGGLNPAFGNEAVND"
     misc_feature    complement(7801..7815)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.2"
                     /tool="antismash"
     misc_feature    complement(7850..7865)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.6"
                     /tool="antismash"
     gene            complement(7936..8214)
                     /locus_tag="KY5_RS14370"
                     /old_locus_tag="KY5_2850c"
     CDS             complement(7936..8214)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018529518.1"
                     /locus_tag="KY5_RS14370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_2850c"
                     /product="hypothetical protein"
                     /protein_id="WP_098247248.1"
                     /transl_table=11
                     /translation="MKSLKAAAVLAGSIALAASAAPAFASDLAPTSLNGGLETITSQDL
                     LASEPLSSNLLDTENGDSVVNTVKDAAGGLNTAGGPAQVLGGLPLAK"
     misc_feature    8407..8420
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.18"
                     /tool="antismash"
     gene            complement(8503..9231)
                     /locus_tag="KY5_RS14375"
                     /old_locus_tag="KY5_2851c"
     CDS             complement(8503..9231)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006382606.1"
                     /locus_tag="KY5_RS14375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_2851c"
                     /product="hypothetical protein"
                     /protein_id="WP_098242621.1"
                     /transl_table=11
                     /translation="MRALPVRRIATTTLCATLLLGTAGPAVAAVAAVGDAGHDETQSAP
                     RAPAPDASALLAQTKQLADVGGVITPVTALLDAVLKADGNKLSPDEAKKHVDAVKAAID
                     AAAKKAPEAPAAKLPATPEAPKLPAAPAVPKLPTGGSERADKAPLDVKADALAALQSAV
                     DALVKAATAGDAKTVLTQAPVVLTSLVNVAVATLLSGGLPAPSLPGLPPLPKLPTGGLP
                     EAPKLPTGALPQTPSLPVAP"
     misc_feature    complement(9360..9374)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.28"
                     /tool="antismash"
     gene            complement(9507..9746)
                     /locus_tag="KY5_RS14380"
                     /old_locus_tag="KY5_2852c"
     CDS             complement(9507..9746)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056482.1"
                     /locus_tag="KY5_RS14380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_2852c"
                     /product="chaplin"
                     /protein_id="WP_098242622.1"
                     /transl_table=11
                     /translation="MSRTTKALALSAVAAAAMAGTAGVAAADSGAQGASSNSPGVISGN
                     TAQVPVHVPVNVCGNSVNVIGLLNPAFGNQCAND"
     misc_feature    9866..9882
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 21.17"
                     /tool="antismash"
ORIGIN
        1 cgtgggcgac gaagtcggcg tcctcgtcgc ccagcgggga gccgctctcc gcgatgccgg
       61 ccaggaccag gcccgccacc tcggcaagca ccgcacggcg cgcctcggcg acggcggccg
      121 cgatggcctc gctgagctcg gacgcctggc ggtgcagcac cacccagctg tcgcggtgct
      181 cggccacgaa ggcgaagaac gccaggagcg ccgcgtgcaa ccggagttcg ggggccgccc
      241 cgcccgtcgc cgccgcgccc tggaacgccg cgacgagccg gtccgcctcg cggcgcagac
      301 aggcgaggaa gaggccctcc ttggaatcga ggtagagata caccatcggc ttggaaattc
      361 ccgcgagttc ggcgatttcg tccaccgaag ccgcgtgata gccacgcttg gcgaagacct
      421 ggaccgccac gtcgatgatc tgctgctcgc gctcgcgcct cgggacgcgc cgcctgccgg
      481 tcacccttgt cagcctcctc ggtccgttct accttactgt ttagtaagtt tactttagag
      541 tcaggagatt gccatggcgg acgggaccgg cggcatcgag gacctcggcg gcgtgcaggg
      601 ccttgccggg ctcgacttcg cgagcgtcac ccccgaggag ttcgcgcaga tcgtgaaggg
      661 cctctccggc aaggagatcg ccgagctcgc ccgggacgtc gagctgcgcg gccgcgtgct
      721 cgccgaggtg ttcacgcgca tggagcggca gttcaggccg gacgcggcgg gctccctcaa
      781 ggcgctcatc cgctggaaga tcaccggcag cggggacaac gaggaggcgg tgtacgagac
      841 ggacatcgcc gacggcacgt gcaccgtccg caagggccgc tccgacgccg acccgcgcgt
      901 caccctgatc ctggccgacg ccgagttcct caaactggtc tcgggcaacg cgagtccggt
      961 cacgatgttc atgacgcgca aggtgaagat cgcgggcgac gtcgcgctcg cctcggggct
     1021 cacgcgctac ttcgacatcc cgaaggcctg agcccaccgg gttcaagcgg ctcccgggcc
     1081 gggccgatgt aacgatcatg acgtctacgc actccccggc ccgaggccgt cccccggaag
     1141 ccgtcgtcgg gctcctgccg accgcgcgcg gcggccggtg ggaccgctgg cgcctgcccc
     1201 tcgcgctcgc gcccgccctg gtgatcctcg tcgccgggtt ccagcggcgc tggatgtcgg
     1261 acgacggaca catctacgta cgcacggtcc ggcagatcct cgcgggcaac ggccccgtct
     1321 tcaacgcggg cgaacgcgcc gagtcgtcca ccggcaccct ctggcagtgg ctgctcgtcg
     1381 ccgcgggcgc gaccggcgcc gacatcgcca ccgccgcgat gtacggcgga ctgctgctca
     1441 ccgccgccgg gttcgcgctg gccgccctcg gcgcactgcg gctgcacggc gcagagcggc
     1501 gggtcggcgc gctgctcccc tgcggcgcgc tcgtgctcct cgcccttccg cccgtctggg
     1561 acttcgccac gtcgggcctg gagaccgggc tcgccacctg ctggctcgcg ggcgcctggc
     1621 tcgcgctcgt cgccgggccg cgcgcctggc cgacctgcgt cgtcctgggc ctcggcccgc
     1681 tggtccgtcc cgacctggcc ctggtctcgc tcgtcttcct ggccgcgcag tggctcatga
     1741 cgcgcccctc ctggcgccgc gcgctcgcgg gcgcggcggc cgcgagcgcg ctgcccgtcg
     1801 cctacgaggt cttccggatg ggctactacg gccacctgat gccgctgccc ggcgtcacca
     1861 aggaggcgtc gcgcagcctg tggacgcggg ggctcgccta tctcgcggac ttcgcgggcc
     1921 cctatctgct gtgggtgccg ctgctcttcc tcgccgccgt cgcgctgccc tcgggccggg
     1981 tgcgccattc ggtccccctg gtccccgcgc tcgcccccgt cgtcgcgggc ctgctgtgct
     2041 gggtgtacgt catcaaggtg ggcggcgact tcatgcacgg gcggatgttc ctgccgggtc
     2101 tcttcctgat gctgctcccc gtcttcctgg tgccgctcac gcgcgcgtgg accatcgccg
     2161 cgctcggcgt cggcatctgg gccctcgcgt gcgcgagcgt cctgcgcatc ccctacgagg
     2221 ggcggatcgg cccctccggc gtcgccgacg aacgaggcgt ctacgtacgg cagaacgcgg
     2281 caccccaccc cctgcaccac gacttcgcgg gcttccccgg caacagggcg tacgcgcgcc
     2341 tcgtgcacga cgcggcggac ggcggggcgc ccgtgctgct gctcggcagg acacccgccg
     2401 acgccggggc gccgggcgtg acggggatct acaacacgct cggcttcagc ggcaccgtcg
     2461 taccgctgag gggcgccgcc ctcgacccga tcggcctcgc ctacccgctc gccgcccact
     2521 ccgagggcat caacggccgg gtggggcacg acaaacggct gcccgacgag tggatctacg
     2581 ccgaccgggg cggcccgggg gcgccgcccg ccggactcga tcccgcgcgg gtcgacgcgg
     2641 cccggcgggc gctgcgctgc ggccccttcg cggaactgcg ggcggccacc cgcgagccgc
     2701 tgacggcggg ccggttctgg cggaacctga cgggcgcggt ggagcggacg tccttccgct
     2761 tccccgacga tccggtgcgg gcggagcggg cggcctgcgg aggcacctcc taagccgacg
     2821 cggcccccga ctgagttcac tcgcctggcc gtactcggcg cgctgtggcg tacgcggtcg
     2881 ggcagcgtgg gccatgtccc tgcacgccct cgttggcgtg tgtacgtccc agtcgaagga
     2941 ttcccggatg aagcgcacag cacgcgccct gacgttcgtc gccctcaccg cggccctgac
     3001 ggtccccacg gtcgggatcg cctccgcgac cgccccggtg gccgcgccca cgaccaccaa
     3061 gagcgccccg agccacgact gggacgacga ctggcaccac taccaccacc actactaccc
     3121 gggcgtcggt gtgggcctgg gtgtcggtct gggcatcctg ctctgacctg gcctgacctc
     3181 acacgcgaga tggccgccac gactgtcgtg gcggccatct cgcgtgtcgg gcgaactact
     3241 gaacggcggg caccgcgttg gtgacgggcg ccaccaggtc cgtgagctgg tgcaggtcgt
     3301 tcaaccgctg gaggccgctc agctggctcg ccacggtggg cagctcgtcc ttgtggtcgt
     3361 ccgggatggc ggtggtgccg aggtcgtcga tggcgctgag cacggacgga gcacccgtcg
     3421 cgtcggcgcg gtcggaggcg tgggctgtcg gcgcgagcag ggcggctgct ccggccgcca
     3481 gcgagacagc agcgatgagt cgtcggggag aagtcacgtt tcctgaaacg gccgagtccg
     3541 ggtaaagaca cggctccggc atttttctca ttcaagaagc gcagaattgc gcagctattc
     3601 gggttttctt ccccgtgaag cctgaagtta ctggattcgg tgggaaaatc acaccgtgac
     3661 ctcgggatga cgatcggcgt tacacgaagt gcgactgcag ctatgcagcc gctctttcga
     3721 gtcaaaggag aacgtgatgt ctcgcatcgc aaaggcagcc gccgtcaccc tcggcacggg
     3781 agccgtcgtg ctcggcggcg ctggtctggc catggcggac gccggcgcgc acggtgaagc
     3841 tgtcggctcc cccggcgtga tctcgggcaa cctgctccag atcccggtca acatcccggt
     3901 caacgtctgc gggaacaccg tgaacggcat cggcgctctc aacccggcct tcggcaacca
     3961 gtgcgccaac acgggcggcg acgacgccgg ctacggcggc tgagttccgt catctcgtca
     4021 gtgagacgct cggccgggat tcccggccga gcgcttctca tgagcggcct tcgaggtcgg
     4081 ccggggcccg gccgggtcag cttccgtacc ggtagagctt cttgtgttcc aggagggcgt
     4141 ccggcttgac gttccagggc ggcatcggct cgtgccgcgc cacgatgcgc cggtactggg
     4201 ccgagccaag ccggggcggc cgctcgggca gatagggggc ggagtccgga tggcggcgct
     4261 gccagcggtc ccacaggaga tccacgtacg cgtggtgcag ccagaacacc gggtcgttca
     4321 ccgaggcccc gcccagcatg tgtccgccca cccagcggtg cacgcggttg tggttgcgcc
     4381 accgctccgt gccgctcgcg gacgtccagc cctccagcct gttgcggaac cctccttccg
     4441 acgtggagtc ccacggctcg gcgtcgtagg cggagtcctc catggcccag gccacgtcgc
     4501 gcgcggtggg caggtcgatg gggtcctggg ccctgcccag gttgcgggtg aggtactcgg
     4561 cgtccgtgac gcctaccttg acggtccagt gaccgctccc gtaggcgaag ggcccggtcg
     4621 tgacctgccc gtccgacggc cgtccgttcc cgcccatcag gtcgtcgccc cacagcgaga
     4681 cggcgggcgt acggtccttc gtccagtccc agtagggcac cgtgacgccc gcgtcgaggc
     4741 gctgtaacgc ccgctcgaac tccagcagga agcggcggtg ccaggggaag aaggacggcg
     4801 tcatgtgggc cacccgcagg ctgcgctccc cgtccgacac gtagtagtcg atgtgcgtgc
     4861 gcacgaactc gtcgtactgc cccgagcgct tgagcttgag caccgcgtcg acgaatctgc
     4921 ggcgctccgc gcgactgagg ctgctctggt tcttgcgcgt atgagccatg tccatccgtt
     4981 ccacgggcgg gcggccgtac tcagtggtga cggccgctca atgtctggtg gctgccgagc
     5041 gcgtcgaccg ccgccctggt ggcgctcagg ggtgtcgcgt gcgactcgta gtgatccacc
     5101 atgctgaggt agctgccgtc ggcgcggcgc atgaggccga gcggccttcc gtcgaccgtg
     5161 acgttccagt cgcctgccgc gtcggcgtcc agaccggccg acggctgccc cacgatgcgg
     5221 cgcccccggt acatctcgtc gaacaacaaa tcgcccgccg gacccgcgag atcgggcgcc
     5281 gaccgcgtct gtgccgtacg gaagattccc gcggcaccca gcagcgacgc gaaggcggtg
     5341 cccagcaggt ggcggcgggt gagccggttg acgaaggact tgcgtggcgc ctgcggggcg
     5401 gccgcgggca gcactgcgag ggcctggtcc acggccgggc cctcgcacgt cgactggggc
     5461 gccatcgtca ggcgagcccg aggttggccg cgggctgcgc gcgcagttcc ggcgcctcga
     5521 tgcccgcgtt gggcagcttc ggcgcgggca ggccccacag ctccggctgc ggggccgtca
     5581 ggggaaggtc caggttgggg gacggcgtga ccgcctcgac ggacgacccc cgggcctcgg
     5641 cgccgacgga ctccacggtg tcctcgccca gcatgtccgt gagcggggcc gaaagctgcg
     5701 tgtcggggag gacgccgctg accgggaagc gcgggaccgc gcgctcgggc agcaggctgt
     5761 cctgtgcgta gcgcggtccg tcgaggccct cgccgggcag gggcaccggg acgcccgtgg
     5821 tgagttcggg ggcctccatg tccagggcgt actcgagccc gttcaacggg acggtcaccg
     5881 agggctgttc cgccgcctgg gccgccgtgc ccgcgcccgc tcccgcggcc gccgcgcagg
     5941 caagggctac ggcaagggca cctctggttg acttcttcat tgctcgattc gtcctttcgc
     6001 tgtgccgatc actcggtcct cgacccgctc aacgagtcgg ccgtgccgga agtgcagaat
     6061 tcccccgact gcaccaatgc gcctgtacgg gaatcccgtt ggagcggact cgggcctcag
     6121 ctgatccaga agtcccacca gcgggtgagg atcagcatgc cgatgacccc catgtggaga
     6181 acgggagccg cccacgagaa ttcggtgagg acgacacgga ttccggcggg tacggggagg
     6241 aatccccggc tgacgttgta cgacgtcacg taccagaaca tgagaagggt ggcggcccac
     6301 gccagacagc accagagaca gagcgaattg atgcggtaca gggactggaa ctgcagccac
     6361 gtgcagaatc cgacgccgaa ggccgtgccc gcgttgaggg tgagccagta gccgcgcggg
     6421 aagcgggccc cggcgagcag gctcatgccg acgcagagca ccacggggta agtggcgagg
     6481 ccgagcaggg ggttggggaa cccgaacgcc gccgcctgct cgctcttcat gatgttgccg
     6541 caggccacca cggggttgag gctgcacccc gggatgaagt ccgggtcttc caggagcgtg
     6601 aacttgtcga gcgtgatgac ccacgccgcc agcagcccga gcgcgccggt gaccaccagg
     6661 agcacgccga gtccgcggct cgccccgctg tccgcggccc accccgggcc cggcgcgggg
     6721 gcagcggcct cgccgggggc gacggggtcg ggttcaccga cggcctgcgg cgggacccgg
     6781 ccgggcgcgg aggtggcgcg catcaggaac gcaggctgcg cgccggcagc aggcagtgcg
     6841 cggcgttgag cagggtctcc tcgtcaccgg cgaactcgct gagcagttcc tcgctgacct
     6901 tctcccccgg cttggcctcg ggccaggccc gggtggtggc catgaagtag gcgtgcccgc
     6961 gctgctgcac ggcggcgagc cactccggcg gcaccgggca ctgcgcgttg acctggggca
     7021 tgttgacgac ggcctggtcg gcctcgacaa ggaggctgac cggcatcgag gggacgcgag
     7081 ctccgtcgac gagcgggccg ccgatgggca ggcccgcgtt gtggagcatc gtggtcagcg
     7141 cctcggcggt ggactccgga ccgccctcgg tgtcgccgag cgagtaggcg agcaggaagg
     7201 gcatgtcctt ctggtcctcg gggtgttcac cgctccaggc gatgaccgcg agggtgccca
     7261 ggtctgccgc gtggaaggga cgctgttcgc tggttgttga ggtcatgggg gaaccgtata
     7321 gaggcgcgga caccgcgccg ggcctttgtt cacccgtgtg ggcgagcccc gtggctccat
     7381 cacacgatgg ggtaacgcgg agggcccacc gaggaggctc ccggtcgaac tggcggtctg
     7441 tcgcggcgcc ttgtccggct ccggacagac cgaagggccg gccccgtgga agaggtggac
     7501 cggcccttgc gccttcggcg cgtgcccgag gcgtccccga gggggtgtcg ttcagtcgtt
     7561 gacggcctcg ttgccgaacg ccgggttcag accaccgatg agggtgccgg tgttgcccgc
     7621 gacgttcacc ggaacgtcga cgggaacctg gcccaggttg ccggagatga cgccggggga
     7681 ctccacggcc gcgccctggg cgctggcacc gtcaccgtcg gtggcgaagg cgctgccgga
     7741 agccgcgccc gcggcgagac cggcagcggc gaggacgagt gcggccttct tggcattctt
     7801 catgatctga accttccctt gggggtttgc tttagcctat ggattcgcct gtgagccgga
     7861 tcgcaacaac acgccgactc tccctctccg gcgttcgatt caattcgcct tacgggaggg
     7921 gaaatcgagg ggcgattact tggcgagggg aagtccaccg agaacctgag cgggcccacc
     7981 ggcggtgttc aggccgcccg cggcgtcctt gaccgtgttg acgacggagt cgccgttctc
     8041 ggtgtcgagc aagttgctgc tgagcggctc cgaggcgagc aggtcctggc tggtgatggt
     8101 ctccaggccg ccgttcaggc tggtcggcgc caggtcgctg gcgaaagcgg gcgcggcgga
     8161 agcggcgaga gcgatcgatc ctgcgaggac agcagcagcc ttcagggact tcatcatgtt
     8221 cctttccttg gcggctattg ccgcgtatgg gggttccgtt caggcgttga ttacctgtcg
     8281 tctctgttca ctcctaccaa cgagcgcagg ccgataagga aactcctggc ggcaggaatt
     8341 ccggcaacgg gaccgaatgg cgagcgcgtc ccatcctcat atcaactggc gtacaagtga
     8401 tccgtgttga ccccggtcat gcgagagccc gccatgccgg gagatcggca tggcgggctc
     8461 tcgtgcgacc ggtggccggg gacgggcctc ggccacctcg cctcagggcg cgaccggcag
     8521 ggacggcgtc tggggcaggg caccggtggg cagcttcggc gcctcgggga gccccccggt
     8581 gggcagcttc ggcagcggcg gaaggcccgg caggctcggc gcgggcaggc cgccgctcag
     8641 gagcgtggcg acggccacgt tgaccaggct ggtgagcacc acgggggcct gcgtgagcac
     8701 cgtcttggcg tcgcccgcgg tggcggcctt gacgagggcg tccaccgccg actgcagggc
     8761 ggcgagcgcg tccgccttga cgtcgagcgg cgccttgtcg gcgcgctcgg agccgccggt
     8821 cgggagcttg ggaaccgccg gcgcggcggg cagcttgggc gcctccggcg tggcgggcag
     8881 cttggcggcc ggcgcttcgg gggccttctt ggccgccgcg tcgatcgccg ccttcacggc
     8941 gtcgacgtgc ttcttggcct cgtcgggaga gagtttgttg ccgtcggcct tgaggacggc
     9001 gtcgagcagt gcggtgaccg gggtgatgac gccaccgacg tcggcgagct gcttggtctg
     9061 ggccagcagc gcggacgcgt cgggggcagg cgcgcggggg gcggactggg tctcgtcgtg
     9121 gccggcgtcg ccgacggcgg cgacggcggc gacggcgggt cccgccgtac cgaggagcag
     9181 cgtcgcgcac agggtggtgg tggcgatacg ccgtacgggc agagcgcgca taggtaaatc
     9241 cctttcggtg atgcgtcgga tcttgtgatc accgtggaat cgacccacgc ccgccgcaac
     9301 cgagcgcctg cggaacatcc cgcccttccc tcgaccggtg cggcatacat gcaggtgagg
     9361 cccggatgtg gtgggtggtc agcggcctgc gccgcgcccg ccattcgggg cacgcgccgc
     9421 tcgcccgacc gggtgcgccc tcggccccac gcacgacggc cgcccctgcg gaagcaggga
     9481 cggccgtcgc ggtggagacg acgcgcttag tcgttggcgc actggttgcc gaaggccggg
     9541 ttcagcaggc cgatgacgtt gacgctgttg ccgcacacgt tgacgggaac gtggaccggc
     9601 acctgggcgg tgttgcccga gatgacgccg ggggagttgc tggacgcgcc ctgcgcgccg
     9661 gagtcggcgg cggcgacgcc ggcggtcccc gccatggcgg cggcggcaac ggcggagagg
     9721 gcgagagcct tggtcgtacg cgacatgggg tactgctcct tgctgggtat tgcgatagat
     9781 gggcggttgc ccgtcctgtg tgtgaacggc cgaccccggt gccaagttgt gcgcccagac
     9841 gggtgatcgt ggcgcgtgtc ccccgtgctg acgaaaccgg cacgcgggcg ttgagcggcg
     9901 tcgccgcacg gcccccgggg gatgtgggcg gcccgtagcg gcaccaccgc cccgccgcct
     9961 ccttcacaat tccgacatac gtcgcggagt tgacggatgt ggctccttca aaccgctaca
    10021 gttgcgcttc ccctcgggtg cattccaccc ataagcccac agaagagcgc attacatgcg
    10081 aagttctcat ctccgctgtg gttcccaggt cgtcacaccc cggaaggcca atgccgtcat
    10141 gcgccattct ctgggcctgc gccgtgtgtt cgtcccgctg ctcgcactca ccgcgtgcct
    10201 caccacgcac ggcaccgggg cagcggcccc acccggtccg cccggcaacg agcgaagccg
    10261 cctcgacttc acccagcgct tccacgcgct gcagcacggc ggcatc
//
