LOCUS       NZ_CP022685            10405 bp    DNA     linear   CON 17-JAN-2024
DEFINITION  Streptomyces formicae strain KY5 chromosome, complete genome.
ACCESSION   NZ_CP022685
VERSION     NZ_CP022685.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces formicae
  ORGANISM  Streptomyces formicae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9611874)
  AUTHORS   Holmes,N.A., Devine,R., Qin,Z., Seipke,R.F., Wilkinson,B. and
            Hutchings,M.I.
  TITLE     Complete Genome Sequence of Streptomyces formicae KY5, the
            formicamycin producer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9611874)
  AUTHORS   Holmes,N.A., Devine,R., Qin,Z., Seipke,R.F., Wilkinson,B. and
            Hutchings,M.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2017) School of Biological Sciences, University of
            East Anglia, Norwich Research Park, Norwich, Norfolk NR4 7TJ, United
            Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.0; Newbler v. 2.3
            Assembly Name                     :: KY5 Genome
            Expected Final Version            :: yes
            Genome Coverage                   :: 56.0x
            Sequencing Technology             :: PacBio; 454; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/17/2024 10:10:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,274
            CDSs (total)                      :: 8,185
            Genes (coding)                    :: 8,082
            CDSs (with protein)               :: 8,082
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 39 of 103
            Pseudo Genes (incomplete)         :: 77 of 103
            Pseudo Genes (internal stop)      :: 11 of 103
            Pseudo Genes (multiple problems)  :: 22 of 103
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:13:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2160651
            Orig. end                         :: 2171056
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022685.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Matthew Hutchings,
            University of East Anglia, UK.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="16"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(300..1397)
                     /locus_tag="KY5_RS08950"
                     /old_locus_tag="KY5_1775c"
     CDS             complement(300..1397)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123619.1"
                     /locus_tag="KY5_RS08950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_1775c"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_098241723.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.1e-15, bitscore:
                     56.6, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDHAVLHVKGRVLVGPEDVRGELWVVGGRVTYDRPVGAAAAEIR
                     TVEGWALPGLVDAHCHVGLDTHGPVDAATAEKQALTDREIGTLLIRDAGSPSDTRWIDD
                     REDLPKIIRAGRHIARTRRYIRNYAHEIEPDDLVAYVAQEARRGDGWVKLVGDWIDRDA
                     GDLTACWPRGAVEAAIAEAHRLGARVTAHCFAEDSLRDLVEAGIDCIEHATGLTEDTIP
                     LFAERGVAIVPTLVNIATFPDLAAGGEVKFPRWSAHMRRLYDRRYDTVRAAYDAGVPVY
                     AGTDAGGSLAHGLVAAEVAELTLAGIPAVDALSATTWGAREWLGRPGLVEGAPADLVVY
                     EADPRADVRVLAAPRRVVLNGRVVG"
     gene            1486..2586
                     /locus_tag="KY5_RS08955"
                     /old_locus_tag="KY5_1776"
     CDS             1486..2586
                     /NRPS_PKS="Domain: Aminotran_5 (52-286). E-value: 7.3e-27.
                     Score: 86.1. Matches aSDomain:
                     nrpspksdomains_KY5_RS08955_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644018.1"
                     /locus_tag="KY5_RS08955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_1776"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_234363153.1"
                     /sec_met_domain="Aminotran_5 (E-value: 6.1e-20, bitscore:
                     70.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAQHFAAIEARVAGLLATEQDVVIMQGEALLPLE
                     GCIRGAARPGTTALNVITGPYGQTFGNWLRDCGATVIDLAVPFHTAVTAEQVRQAFAAH
                     PEIDFVSLVHAEAATGNTNPVAEIGAVVREHGALFMLDAVASVAAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWQRLAANPQAPRRSYLSLLDWKERWIDGGRKALLHAP
                     AQLEMLALEACVERIETEGLDALMARHATAAAATRAGALALGGGLEPYVYEAGDAAPVA
                     TTLRAPSGVDASELVARALAADPVLPLVAGGGALAKEMIRVNHYGPDATRGVVHASLAA
                     LGAAMTESGLTVDLEAARRAVTDAWS"
     aSDomain        1642..2343
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KY5_RS08955_Aminotran_5.1"
                     /evalue="7.30E-27"
                     /label="KY5_RS08955_Aminotran_5.1"
                     /locus_tag="KY5_RS08955"
                     /protein_end="286"
                     /protein_start="52"
                     /score="86.1"
                     /tool="antismash"
                     /translation="RPGTTALNVITGPYGQTFGNWLRDCGATVIDLAVPFHTAVTAEQV
                     RQAFAAHPEIDFVSLVHAEAATGNTNPVAEIGAVVREHGALFMLDAVASVAAEPLLPDA
                     WGVDLCVIGAQKAMGGPAGVSAVSVSERAWQRLAANPQAPRRSYLSLLDWKERWIDGGR
                     KALLHAPAQLEMLALEACVERIETEGLDALMARHATAAAATRAGALALGGGLEPYVYEA
                     GDAAPVATTLRA"
     misc_feature    2723..2736
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.73"
                     /tool="antismash"
     misc_feature    complement(2783..2800)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.86"
                     /tool="antismash"
     misc_feature    2849..2864
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    2977..2995
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.2"
                     /tool="antismash"
     misc_feature    2997..3011
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.38"
                     /tool="antismash"
     gene            3020..3571
                     /gene="ectA"
                     /locus_tag="KY5_RS08960"
                     /old_locus_tag="KY5_1777"
     CDS             3020..3571
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987798.1"
                     /locus_tag="KY5_RS08960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_1777"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_199842990.1"
                     /transl_table=11
                     /translation="MTAAQADLPTDLQAKFQKMPEGLRLDSPVVADGAAIWRIARDSKV
                     LDLNSSYSYLLWCRDFARTSVVARDADGEVAGFITGYIRPDRPGTLMVWQVAVDHPHRG
                     KGLAAALLDGLTAQVTATHPVDSLETTISPDNTASEALFTSYARRHGASVARTVLFDAG
                     LFPDDGHEPEVLYLIEPLPH"
     misc_feature    complement(3054..3070)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.79"
                     /tool="antismash"
     misc_feature    3062..3076
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.64"
                     /tool="antismash"
     gene            3679..4950
                     /gene="ectB"
                     /locus_tag="KY5_RS08965"
                     /old_locus_tag="KY5_1778"
     CDS             3679..4950
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 1.7e-73.
                     Score: 239.5. Matches aSDomain:
                     nrpspksdomains_KY5_RS08965_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="KY5_RS08965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_1778"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_098241726.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.3e-76, bitscore:
                     254.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAQGSRMFDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLETFQNVILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYLDGQTPDFIWFERLLEDSGSGLNTPAAVIVETVQGEGGINVARA
                     EWLRKLADVCERWDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDVWEPGEHNGTFRGNNPAFVTAAAALETYWTDGPAMEKQTLARGQQIEETL
                     SALRAEYPDDTVEYRGRGLVWGIQFRDKERAGKVAKRAFELGLLIETSGPESEVVKLLP
                     ALTITPDELDEGLRTLARAVRETA"
     aSDomain        3763..4776
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_KY5_RS08965_Aminotran_3.1"
                     /evalue="1.70E-73"
                     /label="KY5_RS08965_Aminotran_3.1"
                     /locus_tag="KY5_RS08965"
                     /protein_end="366"
                     /protein_start="28"
                     /score="239.5"
                     /tool="antismash"
                     /translation="FDRAQGSRMFDEDGHEYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMSTTAKRAFLETFQNVILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYLDGQTPDFIWFER
                     LLEDSGSGLNTPAAVIVETVQGEGGINVARAEWLRKLADVCERWDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDVWEPGEHNGTFRGNNPAFV
                     TAAAALETYWTDGPAMEKQTLARGQQIEETLSALRAEYPDDTVEYRGRGLVWGIQFR"
     misc_feature    4991..5004
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.85"
                     /tool="antismash"
     gene            5001..5405
                     /locus_tag="KY5_RS08970"
                     /old_locus_tag="KY5_1779"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019545556.1"
                     /locus_tag="KY5_RS08970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_1779"
                     /product="ectoine synthase"
                     /protein_id="WP_098241727.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.6e-52, bitscore:
                     175.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAASGTWESKRIVLAKERVGFSLHETILY
                     AGTETSMWYANHIEAVVCTKGEAELTDETTGEKYTITPGTMYLLDGHEKHTMRIKEDFH
                     CLCVFNPPVTGREDHDENGVYPLLTEPDEG"
     gene            5470..6369
                     /gene="thpD"
                     /locus_tag="KY5_RS08975"
                     /old_locus_tag="KY5_1780"
     CDS             5470..6369
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976953.1"
                     /locus_tag="KY5_RS08975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_1780"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_098241728.1"
                     /sec_met_domain="PhyH (E-value: 1.8e-37, bitscore: 128.5,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTTDLYPTRGAEEVLIERKDPVVWSEPGTEGPIAAGDLA
                     GFERDGFLAIDQLITPDEVGVYHAELERLMNDPAMREDERSIVEPRTQEIRSLFEVHRI
                     SELFGRLAADPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTKNHDTNGGLMIMPGSHKTFLGCAGATPKDNYKRSLQMQDAGIPSDGT
                     LTHFASDHGIRLFTGEAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENTAVEPFA
                     APVRRPEFIGARDFTPVK"
     gene            complement(6455..7516)
                     /locus_tag="KY5_RS08980"
                     /old_locus_tag="KY5_1781c"
     CDS             complement(6455..7516)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969934.1"
                     /locus_tag="KY5_RS08980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_1781c"
                     /product="alkene reductase"
                     /protein_id="WP_098241729.1"
                     /transl_table=11
                     /translation="MTTAFDPIDLSGSRLGNRIVMAPMTRSRAGEGGVATDLTAEYYAQ
                     RASAGLIVTEGIQPSVVGQGYPDTPGLHSAAQVAGWRKVTDAVHAAGGRIFAQIMHAGR
                     IGHPVLLPEGLVNVAPSPVAAEGQVFTAEGPKEFITPRELTGAEVRATIGDFVTAARNA
                     IDAGFDGVELHGANGYLIHQFLAPGSNRRTDEWGGSPENRVRFAVEVAKAVTAEIGAER
                     TGFRVSPGNPFNDISEPDPEPVYIALAKELAALDLAYLHVMEVGEIRDLSNRLRKEFGG
                     TFVLSPQTEGPTSHGALALVEDGTADLIAFGALFLANPDLPARLRTEGPYNTPDQSTFY
                     GGTEKGYTDYPTL"
     misc_feature    complement(7601..7615)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.31"
                     /tool="antismash"
     gene            7695..8249
                     /locus_tag="KY5_RS08985"
                     /old_locus_tag="KY5_1782"
     CDS             7695..8249
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643608.1"
                     /locus_tag="KY5_RS08985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_1782"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_098241730.1"
                     /transl_table=11
                     /translation="MKADTAPSATAGDPVCTPGSHGVAQAGPVSLAVSRVARLHRVAAG
                     KLLKGLGLYPGQEFVMMHLWDAGPVRQSELIKAVELDPSTVTKMLQRLEQAGHVRRRPD
                     PADRRAVLVEATDASCGLLAQVEGAWAQLEGRTLAGLDAGERVELARLLAKVEASLCVE
                     GEDSRCGALPGAVPGAGSPLS"
     gene            complement(8477..9490)
                     /locus_tag="KY5_RS08990"
                     /old_locus_tag="KY5_1783c"
     CDS             complement(8477..9490)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477735.1"
                     /locus_tag="KY5_RS08990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_1783c"
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="WP_098241731.1"
                     /transl_table=11
                     /translation="MTTEHEDVRELLGAWAVEAVLPGDEHRITAHLPGCAACSAEARRL
                     RDTVRHLEGPPAPPPSDVPHVLSRAFRTRAPALRTAPHAAPYAAAVAGLQALLGELGER
                     GDWETPVAHDWDVHDTVAHLIAADEPLALHLGLHARVPASSVPEGAPWRAAWAARTSDV
                     IAHERLRTPAQTVATWRNQAAGLLAAPAAHDRELAARAAVLMGVRLPVGDHFLVRAFEA
                     WVHADDIGRALDRRVPPPRVPHLWQLVRLAVRILDIALGPQAPTVALTIEGEGGGTEWI
                     LGDTTDAVRAELVLDPVDFCLLIGGRRPPTDIPHGLSGDTSAAHHLLTRAASLSWL"
     gene            complement(9487..10104)
                     /locus_tag="KY5_RS08995"
                     /old_locus_tag="KY5_1784c"
     CDS             complement(9487..10104)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121398.1"
                     /locus_tag="KY5_RS08995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="KY5_1784c"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_098241732.1"
                     /transl_table=11
                     /translation="MKRAFDTADARGGPERPPPDDDLHRRLVYGDEAALADAYAAYGDL
                     VLRVAARVTRSPAAAEDVAQEVFAHLWSRPYAFDARRGSLRAWLSMLAHRRAVDWVRGE
                     ERHRKATHVDETVLRAVPAPGPAPDEAVLEQERSLLLHSALARLPLHQRQVVHLAYFAG
                     RTYRQAAVELGIPEGTAKTRLRTALRTLAETLADPPLRGEIP"
     misc_feature    complement(10093..10109)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.82"
                     /tool="antismash"
ORIGIN
        1 agtggtggaa cccccacagg agaaatggag ccgccgacgg cgcacgccac atggggacat
       61 cacgtgcgcg ccgagggcgt cgccctcctt cgacgaaggg cgacgcggga atctttacgc
      121 actgagagtg aacggtcagc aacctggcgt gagttcactc caaagggagg tttccgacct
      181 tgctttcgaa tcgggaggca cgggcgttcc cttgagcctc tgaagcaacc ctcgtatcgg
      241 ggggtgaagt cgcagaaggg ggtagatcaa cgacccgtga gacctggcgt aacggccggt
      301 caacccacca cccgcccgtt cagcacgacc cgccgaggcg ccgcgagcac ccgtacgtcc
      361 gcccgcggat ccgcctcgta caccacgagg tccgccggcg caccctcgac gagcccggga
      421 cgccccagcc actcccgcgc gccccacgtc gtcgcggaga gtgcgtcgac cgcggggatg
      481 cccgcgaggg tcagttcggc gacctcggcg gcgaccaggc cgtgggcgag ggagccgccc
      541 gcgtcggtgc ccgcgtagac cggcaccccg gcgtcgtacg cggcgcgcac cgtgtcgtag
      601 cggcggtcgt agagcctgcg catgtgggcg gaccagcgcg ggaacttgac ctcgccgccc
      661 gccgcgaggt cggggaaggt ggcgatgttg accagtgtcg ggacgatggc gacgccccgc
      721 tcggcgaaca gggggatcgt gtcctccgtg agcccggtgg cgtgctcgat gcagtcgatg
      781 cccgcttcca ccaggtcgcg cagggagtcc tcggcgaagc agtgcgcggt gacgcgggcg
      841 ccgagccggt gggcctcggc gatcgccgcc tccaccgcgc cgcggggcca gcaggccgtc
      901 aggtcgcccg cgtccctgtc gatccagtcg ccgacgagct tcacccagcc gtcgccgcgc
      961 cgcgcctcct gggcgacgta ggcgaccagg tcgtcgggct cgatctcgtg ggcgtagttg
     1021 cggatgtagc gccgggtgcg ggcgatgtgc cgaccggccc ggatgatctt cggcaggtcc
     1081 tcgcggtcgt cgatccagcg ggtgtcggag ggggagcccg cgtcgcggat cagcagggtg
     1141 ccgatctccc ggtcggtcag ggcctgcttc tcggccgtgg cggcgtccac cggaccgtgg
     1201 gtgtccaggc cgacgtggca gtgcgcgtcg acgaggccgg gcagggccca cccctcgacg
     1261 gtgcggatct cggcggccgc cgcgccgacc gggcggtcgt acgtcacccg ccctccgacc
     1321 acccacagtt cgccccgtac gtcctcgggc cctacgagca cccgcccctt cacgtgcaac
     1381 accgcgtgat cgctcatgaa cagcagccta aaaggcggcc ggtacgctcg gtaccgcagc
     1441 ccgaacgaac tcgtgagcga agtgagcagt gacgtgagca gtactgtgac gcaccccttt
     1501 ctggacctgg cgccgctgag cgcccagcac ttcgccgcca tcgaggcgcg ggtggcgggg
     1561 ctgctcgcca ccgagcagga cgtcgtgatc atgcagggcg aggccctgct gcccctggag
     1621 ggatgcatca ggggcgccgc gcgacccggc acgacggcgc tgaacgtgat cacggggccg
     1681 tacggccaga cgttcgggaa ctggctgcgg gactgcggcg cgacggtgat cgacctcgcg
     1741 gtgccgttcc acacggcggt caccgcggag caggtgcgcc aggcgttcgc cgcgcacccg
     1801 gagatcgact tcgtctcgct ggtgcacgcg gaggcggcga ccggcaacac caaccccgtc
     1861 gcggagatcg gcgcggtcgt gcgcgagcac ggcgcgctgt tcatgctgga cgcggtggcc
     1921 tcggtggcgg ccgagccgct gctgcccgac gcgtggggcg tggacctgtg cgtgatcggc
     1981 gcgcagaagg cgatgggcgg gcccgcgggc gtctccgcgg tgtcggtcag cgagcgggcc
     2041 tggcagcgcc tggccgccaa cccgcaggcg ccgcgccgct cctacctctc cctcctggac
     2101 tggaaggagc gctggatcga cggcggcagg aaggcgctgc tgcacgcgcc cgcccagctg
     2161 gagatgctgg cgctcgaggc gtgcgtggag cggatcgaga cggagggcct ggacgccctg
     2221 atggcgcggc acgccacggc cgcggccgcc acgcgcgcgg gcgcgctcgc gctgggcggc
     2281 ggtctcgagc cctacgtgta cgaggcgggg gacgcggccc cggtcgccac cacgctgcgc
     2341 gcgccttcgg gcgtcgacgc ctccgagctc gtcgccaggg cgctcgccgc ggacccggta
     2401 ctgccgctgg tcgccggggg cggggcgctc gccaaggaga tgatccgcgt caaccactac
     2461 ggccccgacg cgacccgcgg cgtcgtccac gcctcgctcg cggccctggg cgcggccatg
     2521 acggagtcgg gcctcacggt cgacctggag gcggcacgcc gcgcggtgac ggacgcctgg
     2581 agctagccgc cgcgacccgg gggcagggtg cgggtcgcgg gcggccgcgc gttcgtcgtg
     2641 gcttgtcgcg cagttccccg caccccttcc gggactcctc ttccattgaa ttccctcaga
     2701 cgccctcaag gcattatcgc gaattgttta taaacccgag aagcacagct tcccgtattc
     2761 ttctgcccgc tttccgggga cctaaacact aaggtttcaa cagcgccaac caccgtaatt
     2821 cggacccacc ccgcaccagt tgttgatctg tgacgcactc cacatcgcgt ccattttgtg
     2881 cactatctcc tgggcaaatc accataaatg gtcgggatct cgtgcgggcg cgcacccccg
     2941 cgtgataaca catggaccgc tctcacgtaa cccctttgct cgatgcaatc ccaggaattg
     3001 ctcggtaaat tcaatccgca tgaccgctgc acaagccgat ctaccgaccg acctgcaagc
     3061 gaaattccaa aaaatgcccg agggactgcg actcgacagt cccgtggtcg ccgatggcgc
     3121 cgcgatctgg cggatcgccc gcgactcgaa ggtgcttgac ctcaactcct cgtacagcta
     3181 tctgctgtgg tgccgtgact tcgcgcggac ctccgtggtg gcgcgggacg ccgacggcga
     3241 ggtggccggt ttcatcaccg gctacatccg ccccgaccga cccggcaccc tgatggtctg
     3301 gcaggtggcc gtcgaccacc cgcaccgcgg caagggcctc gccgccgccc tcctcgacgg
     3361 gctgaccgcg caggtgaccg ccacgcaccc ggtggactcc ctggagacga ccatctcgcc
     3421 cgacaacacg gcgtccgagg ccctcttcac ttcgtacgcg aggcggcacg gcgcatcggt
     3481 cgcgcgcacg gtcctgttcg acgcgggcct gttccccgac gacgggcacg agcccgaggt
     3541 gctctacctc atcgagccac tgccgcactg agcgacgacg gacggcgggc gcccgcccgg
     3601 ctccccgccc cactcccccg gctttccctc aacacccccc acctcccccg cactacctca
     3661 tctcccagga gattcgctgt gaccatcacc cagcccgacc tgagcgtctt cgagaccctg
     3721 gagtcggagg tgcgcagcta ctgccgcggt tggcctacgg tcttcgaccg cgcgcagggc
     3781 agccgcatgt tcgacgagga cggccacgag tacctcgact tcttcgccgg agccggttcg
     3841 ctcaactacg gccacaacaa cccggtcctg aaacgcgccc tgatcgacta catcgagcgc
     3901 gacggcgtca cgcacggcct cgacatgtcg acgaccgcga agcgcgcctt ccttgagacc
     3961 ttccagaacg tcatcctgcg cccccgcgac ctgccgtaca aggtcatgtt cccgggcccg
     4021 acgggcacca acgccgtgga gtcggcgctg aagctggccc gcaaggtcaa gggccgcgag
     4081 tcgatcgtgt cgttcacgaa cgccttccac ggcatgtcgc tcggctcact cgcggtgacc
     4141 ggcaacgcct tcaagcgggc gggcgccggc atcccgctgg tgcacggcac gccgatgccg
     4201 ttcgacaact acctcgacgg ccagaccccc gacttcatct ggttcgagcg cctcctcgag
     4261 gactccggct cgggcctgaa caccccggcg gccgtgatcg tcgagacggt gcagggcgag
     4321 ggcggcatca acgtcgcccg cgccgagtgg ctgcgcaagc tcgccgacgt gtgcgagcgc
     4381 tgggacatgc tgctcatcgt cgacgacatc cagatgggct gcggccgcac cggcgcgttc
     4441 ttctccttcg aggaggcggg catcacgccg gacatcgtga cggtgtcgaa gtccatcagc
     4501 ggctacggcc tgcccatgtc gctctgcctg ttcaagccgg agctcgacgt gtgggagccg
     4561 ggcgagcaca acggcacctt ccggggcaac aacccggcct tcgtcacggc cgccgccgcc
     4621 ctggagacgt actggaccga cggccccgcg atggagaagc agaccctggc ccgcggccag
     4681 cagatcgagg agaccctcag cgcgctgcgc gccgagtacc ccgacgacac cgtcgagtac
     4741 cgcgggcgcg gcctggtgtg gggcatccag ttccgcgaca aggagcgggc gggcaaggtg
     4801 gccaagcgcg ccttcgagct cggcctgctc atcgagacct ccggccccga gagcgaggtc
     4861 gtcaagctcc ttccggcgct gacgatcacg cccgacgagc tggacgaggg gctgcggacg
     4921 ctggcacgcg ccgtgcgcga gaccgcttga cggccgccgt cccctcaccg tacgacagtt
     4981 aagaaagcag gcaactcacc gtgatcgtcc gttcgttcaa ggacattgaa ggcaccgacc
     5041 ggcatgtgaa ggccgcatcg ggcacctggg agagcaagcg catcgtcctc gccaaggagc
     5101 gcgtggggtt ctcgctccac gagaccatcc tgtacgcggg tacggagacg tcgatgtggt
     5161 acgcgaacca catcgaggcc gtcgtctgca ccaagggcga ggccgagctg accgacgaga
     5221 cgaccggcga gaagtacacg atcacgccgg gcaccatgta tctgctcgac gggcacgaga
     5281 agcacaccat gcggatcaag gaggacttcc actgcctgtg tgtcttcaac ccgcccgtca
     5341 ccggccgtga ggaccacgac gagaacggcg tgtacccgct gctcacggag ccggacgagg
     5401 gctgagaccg gtccaccggt ccgctgacat cccgaacagg gccgtacgag aggagaggca
     5461 ggcaccacca tgaccaccgc acccgaacgc accaccgacc tgtacccgac ccgaggcgcc
     5521 gaagaggtgc tcatcgaacg caaggacccc gtggtgtggt ccgagccggg taccgaaggg
     5581 ccgatcgcgg ccggggatct ggcggggttc gagcgggacg gtttcctggc gatcgaccag
     5641 ttgatcaccc cggacgaagt gggcgtgtac cacgccgagc tggaacggct catgaacgat
     5701 cccgcgatgc gggaggacga gcgttccatc gtcgagccgc gcacgcagga gatccgctcc
     5761 ctcttcgagg tgcaccggat cagtgagctc ttcggcaggc tggccgccga cccgcgcgtg
     5821 gtgggcaggg cccggcagat cctcggctcg gacgtgtacg tccaccagtc gcggatcaac
     5881 gtgaagccgg gcttcggcgc ctccgggttc tactggcact cggacttcga gacctggcac
     5941 gcggaggacg gcctgccgaa catgcggacc gtctcggtgt cgatcgcgct gacgaagaac
     6001 cacgacacca acggcggcct catgatcatg ccggggtcgc acaagacgtt cctcggctgt
     6061 gcgggcgcga cgccgaagga caactacaag cggtcgctgc agatgcagga cgcgggcatc
     6121 ccttcggacg ggacgctgac ccacttcgcg tccgaccacg ggatccggct cttcaccggt
     6181 gaggccggtt ccgcgacgtg gttcgactgc aacgcgatgc acggttcggg ggacaacatc
     6241 accccctatc cgcggtcgaa cgtcttcatc gtgttcaaca gcgtggagaa cacggcggtc
     6301 gagccgttcg cggcaccggt acgacggccc gagttcatcg gtgcgcggga cttcacgccg
     6361 gtgaagtagc ctgccgcgta cgtcagtcgg cccggcgccc cccgtgggtg ccgggccgac
     6421 tcgcgtacgg ccggtacgtc gcgtaccgcc ggtgctacag cgtcgggtag tccgtgtagc
     6481 ccttctccgt gccgccgtag aaggtcgact ggtcgggtgt gttgtagggg ccctcggtcc
     6541 gcaggcgggc aggcaggtcg gggttggcga ggaacagcgc gccgaaggcg atcaggtccg
     6601 cggtgccgtc ctcgaccagg gcgagcgcgc cgtggctggt ggggccctcg gtctgcgggc
     6661 tcaggacgaa cgtgccgccg aactccttgc gcagcctgtt ggagaggtca cggatctccc
     6721 ccacctccat gacgtgcaga tacgccaggt cgagcgcggc cagctccttc gcgagggcga
     6781 tgtacacggg ctccgggtcg ggctcggaga tgtcgttgaa ggggttgccg ggcgagacgc
     6841 ggaagccggt gcgctcggcg ccgatctccg cggtgacggc cttggcgacc tcgacggcga
     6901 agcggacgcg gttctcgggc gagccgcccc actcgtccgt gcgccggttg gagcccgggg
     6961 cgaggaactg gtggatcagg tagccgttgg ccccgtgcag ctcgacgccg tcgaaacccg
     7021 cgtcgatcgc gttgcgcgcg gcggtgacga agtcgcctat ggtggcgcgc acttcggcgc
     7081 cggtcagctc gcgcggggtg atgaactcct tgggcccctc ggccgtgaag acctggccct
     7141 cggcggcgac cggcgagggg gcgacgttca cgagcccctc gggcagcagc acggggtggc
     7201 cgatgcggcc cgcgtgcatg atctgggcga agatccggcc gcccgccgcg tgcacggcgt
     7261 cggtgacctt gcgccagccc gccacctgcg ccgcgctgtg caggcccggt gtgtccgggt
     7321 atccctggcc gaccacggaa ggctggatgc cctcggtgac gatcagtccg gcggaggcgc
     7381 gctgggcgta gtactcggcg gtcagatccg tggccacacc gccctcacca gcgcgactgc
     7441 gggtcatggg ggccatgacg atgcggttgc cgaggcggct gcctgacagg tcgatcgggt
     7501 cgaacgcggt ggtcatcagg actcccgggc aggttgttat gtggtcggcc aagtatctgc
     7561 gccggatgcc actgtaaccc attcattggc cgaccaaggt aatcttctgg aagacatact
     7621 gacgacatac tgaggacgta tctggagatg cgccgagagc accccgcgac cagcagcagg
     7681 agcaggagca cgcgatgaag gccgacaccg caccgtccgc aacggccggc gaccccgtct
     7741 gtacccccgg ctcgcacggg gtggcgcagg cggggcccgt cagcctcgcc gtcagccggg
     7801 tggcgcggct gcatcgcgtg gccgcgggca agctgctcaa ggggctcggg ctgtaccccg
     7861 gccaggagtt cgtgatgatg cacctgtggg acgcggggcc cgtgcgccag tccgaactca
     7921 tcaaggcggt cgagctcgac ccgtccacgg tcacgaagat gctccagcgg ctcgagcagg
     7981 ccgggcatgt gcggcggcgg cccgatccgg cggatcggcg ggccgttctc gtcgaggcca
     8041 ccgatgcgag ttgcgggttg ctggcccagg tggagggggc gtgggctcag ttggaggggc
     8101 ggacgcttgc ggggctggat gcgggggagc gggtggagtt ggctcggctg ctggccaagg
     8161 ttgaggcgag tctttgtgtg gagggggagg attcgcggtg cggggccttg ccgggggccg
     8221 tgccgggggc tgggtcgccg ttgagttgac gtcagccgtg cgtttgcgca gttccccgcg
     8281 cccctgactg cgacgttgcc tgtgacctcg tccagtgggc ctgagccgtg cttctcgcgc
     8341 agttccccgc gcccctggct gcgacgttgc ctgtgtcgtc gttgagttga cgtcagacgt
     8401 gcttctcgcg cagttcctcg cgccccttat ccgcgacgtc gcagcgtggg tgtcagtcgt
     8461 gtctgggtga gtggggctat agccacgaca gggaggctgc tcgggtgagg aggtggtggg
     8521 ctgctgatgt gtcgcccgac aggccgtggg ggatgtcggt cggggggcgg cggccgccta
     8581 tcaggaggca gaagtcgacc gggtccagga ccagttcggc gcggacggcg tcggtcgtgt
     8641 cgcccaggat ccactccgtg ccgccgccct ccccctcgat ggtgagcgcc accgtcggtg
     8701 cttgcgggcc gagggcgatg tcgaggatgc gcaccgcgag cctgacgagc tgccacaggt
     8761 gcgggacccg cggtggaggc acccgccggt cgagcgccct gccgatgtcg tcggcgtgca
     8821 cccacgcctc gaaggcccgc accaggaagt ggtcgccgac cggcagccgt acgcccatca
     8881 acaccgcggc gcgcgcggcc agttcacggt cgtgggcggc gggtgccgcc agcaggcccg
     8941 cggcctggtt gcgccaggtg gcgacggtct gcgcgggagt gcgcagccgc tcgtgggcga
     9001 tgacgtcgga ggtgcgcgcc gcccaggccg cccgccacgg cgccccctcc ggcaccgagg
     9061 aggcgggcac gcgcgcgtgc agcccgaggt ggagcgcgag cggttcgtca gcggcgatca
     9121 ggtgcgcgac ggtgtcgtgc acgtcccagt cgtgcgcgac cggcgtctcc cagtcgcccc
     9181 gctcgcccaa ctcccccagg agcgcctgga gcccggcgac cgcggccgcg tagggcgcgg
     9241 cgtgcggggc ggtgcgcagg gcgggggcgc gggtgcggaa cgcgcgggaa agcacgtgcg
     9301 ggacgtcgga cggcggcggg gccggtggcc cctccaggtg ccgcaccgtg tcccgcagcc
     9361 tgcgcgcctc cgcggagcag gccgcgcagc cgggcaggtg ggccgtgatc cggtgctcgt
     9421 ctccgggcag gaccgcttcc accgcccagg ctccgagcag ttcgcgtacg tcctcgtgtt
     9481 ccgtggtcac gggatctcac ctcgcagggg cgggtccgcg agggtctctg ccagggtgcg
     9541 cagggccgtc cgcaggcggg tcttcgcggt gccttcgggg atgccgagct cgaccgcggc
     9601 ctgacggtac gtccggcccg cgaaataggc caggtgcacc acctgccgct ggtgcagcgg
     9661 gagccgggcg agcgcggagt gcagcaggag cgagcgttcc tgttcgagga cggcctcgtc
     9721 gggggcgggt cccggggcgg ggacggcgcg cagcaccgtc tcgtccacgt gcgtggcctt
     9781 gcggtgccgt tcctcgccgc gcacccagtc cacggcccgc cggtgggcga gcatggagag
     9841 ccaggcgcgc aggctgccgc ggcgcgcgtc gaaggcgtac ggcctgctcc agaggtgggc
     9901 gaagacctcc tgcgcgacgt cctccgcggc ggcggggctg cgggtgaccc gggcggcgac
     9961 gcggagcacg aggtcgccgt aggcggcgta ggcatcggcg agcgcggcct cgtcgccgta
    10021 gacgaggcgc cggtgcaggt cgtcgtcggg cggcggccgc tcggggccgc ctcgcgcgtc
    10081 cgccgtgtcg aacgcccgct tcatcggcac caccacctcg cgcggccgcc ctgcgcgccc
    10141 gccccgccct gccaagcgtg gcgtccagcc gcccggcgcg ccagggttca gccggagagc
    10201 acgtcgagca gccggtccac atccgcctcg gtgttgtaca ggtggaacgc ggcgcgcagg
    10261 tgtcccgcgc gtgcggaggt gacgaccccg gcgcgggcca gttcgggctg ccggtcgccg
    10321 aggcccggca cggagacgat gggggacgtg ccgggcaccg gggtgcggcc gatcctggcg
    10381 agccccgcgc ggtagcgggc ggcga
//
