LOCUS       NZ_NUEG01000032        10264 bp    DNA     linear   CON 30-JUL-2024
DEFINITION  Bacillus wiedmannii strain AFS005864
            AFS005864_61_A10_Contig17_140312, whole genome shotgun sequence.
ACCESSION   NZ_NUEG01000032
VERSION     NZ_NUEG01000032.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus wiedmannii
  ORGANISM  Bacillus wiedmannii
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 415820)
  AUTHORS   Bleich,R.M., Kirk,G.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 415820)
  AUTHORS   Bleich,R.M., Kirk,G.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Assembly Cell v. 4.2.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 37.62x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002555855.1-RS_2024_07_30
            Annotation Date                   :: 07/30/2024 04:09:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,628
            CDSs (total)                      :: 5,554
            Genes (coding)                    :: 5,373
            CDSs (with protein)               :: 5,373
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 181
            CDSs (without protein)            :: 181
            Pseudo Genes (ambiguous residues) :: 0 of 181
            Pseudo Genes (frameshifted)       :: 56 of 181
            Pseudo Genes (incomplete)         :: 126 of 181
            Pseudo Genes (internal stop)      :: 39 of 181
            Pseudo Genes (multiple problems)  :: 35 of 181
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:21:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 116900
            Orig. end                         :: 127164
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NUEG01000032.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10264
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10264
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10264
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5264](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5264
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(145..1578)
                     /locus_tag="CN905_RS19815"
                     /old_locus_tag="CN905_19820"
     CDS             complement(145..1578)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (116-465). E-value:
                     2.1e-35. Score: 114.4. Matches aSDomain:
                     nrpspksdomains_CN905_RS19815_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213549.1"
                     /locus_tag="CN905_RS19815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN905_19820"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_097917000.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.5e-34, bitscore:
                     114.5, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTNKKLPKYRQIIDYMKEKIENGEWPIGSKIPSQRQLAKLFQVN
                     RSTVITALDELMADGLIQGKIGAGTIVTNNTWSLLATNPPPDWSEYVKAGIHKPSKLMV
                     REINEAEANKKLIHLSKGELAPQLFPLETMQSIMKSVCEELEYFGYEEQKGFLPLREAI
                     SKYVKSFGIHTSASSILIVSGALQALQLISIGLLHRESTVLLEQPSYLYSLHVFQSANI
                     NLSGISMDHHGILPNELLKRIKYIQKKNILYSIPSFQNPTGTLMSQERRKEIIKICKKE
                     QLPIIEDDIYRELWIDEPPPAPLKSIDKHGHVLYVGSLSKTLSPGLRIGWIIGPEPVID
                     RLSDIKMQTDYGSSSLSQRVAAEWIHKGYYEEHVANVRIQLKERRRIMIRALNKYCADI
                     ASWDIPSGGLFIWLKVVPSIPMKKLFSEALSNGILLNPGRIYEEESDRYIRLSYGYASP
                     EQMTNGIKLLSELIRKLMT"
     aSDomain        complement(184..1230)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CN905_RS19815_Aminotran_1_2.1"
                     /evalue="2.10E-35"
                     /label="CN905_RS19815_Aminotran_1_2.1"
                     /locus_tag="CN905_RS19815"
                     /protein_end="465"
                     /protein_start="116"
                     /score="114.4"
                     /tool="antismash"
                     /translation="IHLSKGELAPQLFPLETMQSIMKSVCEELEYFGYEEQKGFLPLRE
                     AISKYVKSFGIHTSASSILIVSGALQALQLISIGLLHRESTVLLEQPSYLYSLHVFQSA
                     NINLSGISMDHHGILPNELLKRIKYIQKKNILYSIPSFQNPTGTLMSQERRKEIIKICK
                     KEQLPIIEDDIYRELWIDEPPPAPLKSIDKHGHVLYVGSLSKTLSPGLRIGWIIGPEPV
                     IDRLSDIKMQTDYGSSSLSQRVAAEWIHKGYYEEHVANVRIQLKERRRIMIRALNKYCA
                     DIASWDIPSGGLFIWLKVVPSIPMKKLFSEALSNGILLNPGRIYEEESDRYIRLSYGYA
                     SPEQMTNGI"
     gene            1730..2641
                     /locus_tag="CN905_RS19820"
                     /old_locus_tag="CN905_19825"
     CDS             1730..2641
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551598.1"
                     /locus_tag="CN905_RS19820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN905_19825"
                     /product="DMT family transporter"
                     /protein_id="WP_097916998.1"
                     /transl_table=11
                     /translation="MHNKKWDLRIICAHAFTILIWGTAFPAIRMGLESYTPEHLTLLRL
                     LIASFILLLFSFIYKLRLPDLKDIPAIFMFGALGFTIYHIALNYGEKTVNAGSASLIVS
                     VTPIVTAIFASVFLNEKMKLNGWIGGVISFIGIAFISFSQGDAIQLNSGGLFILLAAIS
                     ESLFFVFQTSYLKKYGFLLFTIYTILSSTVCMFIFLPGIYTEMLAAPLAVNVSVIYLGL
                     FPTVLPYIALAYIISHAGAAEATSSLYLTPVTACFIAWIWLGEVPTFVSIIGGGITILG
                     VLIAHISLKKEIQNEEYCNTAR"
     gene            2837..3760
                     /locus_tag="CN905_RS19825"
                     /old_locus_tag="CN905_19830"
     CDS             2837..3760
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="CN905_RS19825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN905_19830"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_097916996.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRKDIPVISGATKPLTEKITTYYPEIHGPEGLGPIHVPESVLSIPIYNY
                     GSIASILVKYGKDLTIVDVGRSTSLAIAFNLWNDLMLNVKGIYFMGGVFLEVGNVTPLA
                     EANVYGDPIASKIVFQQTKNLLIFPLNVTNKAVLTPNVLNYIQSNSKNPFHTIMKPMYD
                     YYFSAYQKLNPSIEGPLLHDVVAISGLVNPSFFKFVHRTVNVDTFGDTKGQTFADFRPS
                     SISEGAHIALEIDEQKFIQDFIKIML"
     gene            3837..4295
                     /locus_tag="CN905_RS19830"
                     /old_locus_tag="CN905_19835"
     CDS             3837..4295
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="CN905_RS19830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN905_19835"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_097916994.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRENWEAVLKLQVKENQTKFVPAVAVSLAKVYIKPDG
                     DNVEYIPFSIYDGDLMVGFIMHAVVRETADMYWINGFIIDQKQQGNGYGKAALQESISL
                     IKNTCKACKEIRLTVHKDNVFAKKLYERYGFQPLGHEYDGEQVYRLFV"
     gene            4356..4802
                     /locus_tag="CN905_RS19835"
                     /old_locus_tag="CN905_19840"
     CDS             4356..4802
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="CN905_RS19835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN905_19840"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_097916992.1"
                     /transl_table=11
                     /translation="MKNKFHHIVRAVMLKDNKLLLAEYIGHHYFLPGGHVEGGESAEKA
                     LIRELREELGVNCSIKQFLGVIENQWQDHEVLHHEINHIFEVDSQELHIDFIPKSKESH
                     LAFHWIDYNQEALNTYKIMPVPSVKELLERKLSDELLNCWISNF"
     gene            5001..5264
                     /locus_tag="CN905_RS19840"
                     /old_locus_tag="CN905_19845"
     CDS             5001..5264
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071342.1"
                     /locus_tag="CN905_RS19840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN905_19845"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_060486806.1"
                     /sec_met_domain="TIGR03601 (E-value: 3.1e-41, bitscore:
                     134.5, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQSLNLNDYQSGNVVYWDQLQQNQYPYYYIQDDARRCG
                     GCGGCGGRCGGCGGGRCGGCGGRCVGCAGCFGCFNCWNWWII"
     gene            5366..6382
                     /locus_tag="CN905_RS19845"
                     /old_locus_tag="CN905_19850"
     CDS             5366..6382
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="CN905_RS19845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN905_19850"
                     /product="serine hydrolase"
                     /protein_id="WP_097916991.1"
                     /transl_table=11
                     /translation="MYTYDKLISWVENIKEKNHSSATALCIIKDNKIVLEHYNGYHSNT
                     SSNKKVTASSQFNVASARKSYLGLMIAYALYEGKINSIDDEAIKYFNDFDPVLLGKTTI
                     RHLVTHSHGLGETNEGTIFREFEPGQAWAYRDINVRMITRLIYQLYNKSFPELLKERVF
                     DLANFKETGWRIQQDENLVKVVDNPNEDAISEIGTVDDGTEKNLFVSAREFAYWGNLHL
                     NQGVINGKKIVPKEVIEIATSLQSPTFTNKELPQNGLFWFVQNEPALLSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFRNNNRA"
     gene            6414..6557
                     /locus_tag="CN905_RS29460"
                     /old_locus_tag="CN905_19855"
     CDS             6414..6557
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="CN905_RS29460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN905_19855"
                     /product="hypothetical protein"
                     /protein_id="WP_097916989.1"
                     /transl_table=11
                     /translation="MKDEVSKNKKQNIKDVITEQNAIYSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            6837..8045
                     /locus_tag="CN905_RS19855"
                     /old_locus_tag="CN905_19860"
     CDS             6837..8045
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="CN905_RS19855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN905_19860"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_098085775.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAAKLQQKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGSLIDSLGVS
                     QAFLYWGIIVLLLVLTGSFFLREAIVSNIATETLHNDYTPREMMRTKQVYLLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAIFNTVGRIVLGTLSDKIGRMKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGAFIGALLGGFQPTFIIIGVLSVISFVISILIRPPKAEKKKETKHLYR
                     KVA"
     misc_feature    complement(8246..8262)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 24.49"
                     /tool="antismash"
     gene            8335..8961
                     /locus_tag="CN905_RS19860"
                     /old_locus_tag="CN905_19865"
     CDS             8335..8961
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237130.1"
                     /locus_tag="CN905_RS19860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN905_19865"
                     /product="cadmium resistance transporter"
                     /protein_id="WP_097916985.1"
                     /transl_table=11
                     /translation="MVTTIISSFIAFISTNIDDIFILLVLFSQVRTGVIKKEGRAVREK
                     TKMKELYIVIGQYLGFSVIIFLSIMGSLSSFFISISWIGLLGFVPIYMGVKGLLSLRSY
                     KGNEVIANASSSLFKVASITLANGADNISIYIPMFASQNLKTNIVTVVIFFCMIAIWCF
                     ISYTLLRAPILAKVLERNCHIIVPIVLIGLGMFILFRSNTIELLY"
     gene            9151..9567
                     /locus_tag="CN905_RS19865"
                     /old_locus_tag="CN905_19870"
     CDS             9151..9567
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016104977.1"
                     /locus_tag="CN905_RS19865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CN905_19870"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_097916983.1"
                     /transl_table=11
                     /translation="MKIVKQWVQEDSDYIREKVIEYNQKHISEEEKKPSEKISFIVRNE
                     KEKIVGGITAITFWHHIHVDFLWVSEEYRHEGYGSKLLKLIEEFAIENECRLINLDTFS
                     FQAPDFYKKHGYKVIGVSEDHPKGHNHYYLEKRL"
ORIGIN
        1 ggtttaaaaa cagaacctgc ttttgctcaa gtattaaata ttgacggaga tccttatgac
       61 gaacttattg tattgggaca ggaaatgaga ttgtggtaaa cacgatacac aaaaactcct
      121 ctataaataa aagaggagtt tttgttatgt cattaactta cggattaatt cactcaacaa
      181 tttaatcccg ttagtcattt gctctggtga agcatatcca tatgataagc gaatgtaccg
      241 atctgattct tcttcataaa tacgtcctgg gtttaaaaga attccatttg atagtgcctc
      301 tgaaaataat ttcttcatcg gaatgctagg tacaaccttt agccatataa ataatccacc
      361 acttggaata tcccacgatg caatgtctgc acaatattta tttaaagctc gtatcatgat
      421 tcgtcttcgt tcttttagtt gaattcttac gtttgcaaca tgctcctcat aataaccttt
      481 atgtatccat tcagcagcaa ctctttgaga taaagagctc gatccatagt ctgtttgcat
      541 ttttatatct gacaatctat caataactgg ttcgggtcca atgatccatc caattctaag
      601 tcctgggcta agtgttttag aaagactacc aacatataat acatgtccat gtttatctat
      661 cgactttaaa ggagctggag gtggttcatc aatccataac tctcggtaaa tatcatcttc
      721 aattataggt aactgttcct ttttacatat ttttattatt tcttttctcc gttcttgaga
      781 cattaatgtc cctgttgggt tttgaaaact agggatagaa tataaaatgt ttttcttttg
      841 aatgtattta atgcgtttta ataattcgtt aggtaaaata ccgtgatgat ccatagatat
      901 accagataga tttatatttg ctgattgaaa tacatgaagt gaatataagt aagaaggttg
      961 ttcaagtaaa acggttgatt ctctatgcaa aagaccaatt gatattaatt ggagtgcttg
     1021 taatgcacca gagacgatta atatagaact ggctgatgta tgtattccga atgattttac
     1081 gtatttacta attgcttcac gtaacggtaa aaaacctttt tgttcttcgt atccaaaata
     1141 ctctaattcc tcacatacgc ttttcataat cgactgcata gtttctagcg ggaaaagttg
     1201 aggcgcaagt tcacctttac taagatggat aagtttttta tttgcttctg cttcattgat
     1261 ttctcgtacc attaacttgc taggtttatg aataccagct tttacatatt cactccaatc
     1321 aggcggggga ttcgtcgcta ataatgacca tgtattattt gttacaattg ttcctgctcc
     1381 aattttccct tgaatcaatc catctgccat aagttcatct agtgcagtta ttacagtgct
     1441 acgatttact tgaaataatt ttgctaattg cctttgacta gggatcttac ttccaattgg
     1501 ccattctcca ttttcgattt tttctttcat atagtctatt atttgcctat atttaggcag
     1561 ttttttattc gttgtcataa aaaaactccc tcgttcactt taaaaatgcc aagtggttgg
     1621 tttttacgat aaataactgg ttgaagacat tgtatcaact attaaatatg atgaaaagaa
     1681 ataattggta atttgaaagt ggttggttta ttagggaggg agagataaca tgcataataa
     1741 aaaatgggat ttacgcatca tatgtgctca cgcttttacg attctaatat ggggaaccgc
     1801 ttttccggca attcgtatgg gactcgaatc gtatacacct gagcatctta ctttactacg
     1861 tttattaatt gcttcattta tacttctttt gttttccttt atatataagt tacgactacc
     1921 agatttaaag gatatcccag caatttttat gtttggtgct ttagggttta ctatttatca
     1981 cattgcatta aactacggtg aaaaaacagt aaatgctgga tctgcaagtt taattgtttc
     2041 agtcacacct atagtaacgg cgatttttgc ttctgttttt ttgaacgaaa aaatgaaatt
     2101 aaatggttgg attggtggtg taattagttt tataggaatt gctttcatat catttagtca
     2161 aggagatgca attcaattga atagtggggg attatttata ttattagcag cgatttcaga
     2221 aagcctattt ttcgttttcc aaacttctta cttaaaaaag tacggcttct tgctatttac
     2281 catatataca attttatcta gtactgtatg tatgtttatt ttcttaccgg gaatttatac
     2341 agaaatgcta gcagctcctc ttgcagtgaa tgtaagtgtt atatatttag gcttatttcc
     2401 aacagtactc ccatatattg cattagctta tatcatatct catgctggcg ctgctgaggc
     2461 aacaagctct ctatatttaa ctccagtaac tgcatgtttt attgcttgga tatggttagg
     2521 tgaagtgccg acttttgttt cgataatcgg cgggggaatt acaatacttg gagttttaat
     2581 tgcacatata tcattaaaga aagaaataca aaatgaagag tactgcaata cagcaaggta
     2641 gtttgattac tatttgtaag aaattttatt gaactgttaa ttaaattact tatttgagaa
     2701 agaactaaca taatgttagt tctttttatt tatacaatgc ttactagtaa catccatata
     2761 aaattgtata gtttaattat ttttttacat atacatagtt atcatctgct attttctata
     2821 tggaaggagg catcctttgg aaaaagtact gtttttcgga gaccctggta ttgatgactc
     2881 gtttgccatt atgtatggtt tactgcatcc cgaaattgaa attgtcggta ttgttactgg
     2941 atacggaaat gttgagcata tacatgctgc acataatgca gcgtatattt tacagttagc
     3001 aaatagaaaa gacattcctg tgattagcgg tgcaacaaaa ccacttacag aaaaaattac
     3061 aacatactat cctgaaattc atggaccaga aggattaggg cctattcatg tgccagaaag
     3121 tgtattgtct attccgatat acaattatgg tagtattgct tcgattcttg taaagtacgg
     3181 aaaagattta acaattgtcg atgtaggaag atcgacctcg ttagcgattg cttttaattt
     3241 atggaatgat ttaatgctaa atgtaaaagg aatatatttt atgggtggtg tctttttaga
     3301 agtaggaaac gttacgccat tagcggaggc aaacgtatat ggagatccta tagcaagtaa
     3361 aattgttttt cagcaaacaa agaatttatt gatatttcct ttaaatgtaa caaataaagc
     3421 tgtgttaaca ccaaatgtat taaactatat ccaatctaat tcaaagaatc cattccatac
     3481 gataatgaaa ccaatgtatg attactactt ttctgcatac caaaaactta atccatctat
     3541 cgaagggcct ttgcttcatg atgtggttgc tataagtgga ttagtaaatc caagtttttt
     3601 taaatttgtg catcgtacag tgaatgtaga tacattcgga gatacgaaag gacaaacatt
     3661 tgctgatttt cgccctagct ctatatctga aggagcccat atagctttag agatagatga
     3721 acaaaaattc attcaagact ttataaaaat catgttataa gttgttgtcg aaaaaggaaa
     3781 ttatttcact ctgttgaatt taaaaatata ttctctgtat aaaggagctt acactattga
     3841 atgtccagtt aaaagttgtt acgagagaga attgggaagc tgtcttaaaa ctacaagtta
     3901 aagaaaatca gacgaaattt gttccagctg tagcagtctc acttgctaaa gtatatataa
     3961 aaccagatgg tgataatgta gagtacatac cattttcgat atacgacggc gatctaatgg
     4021 ttggttttat tatgcatgct gttgtaagag agacagctga tatgtattgg ataaacggat
     4081 ttattatcga tcaaaaacag caaggtaatg gttatggtaa ggcggcatta caagaaagta
     4141 tttccttaat aaaaaataca tgtaaggcgt gtaaagaaat tagattaaca gtacataaag
     4201 ataatgtctt tgcaaagaaa ctatatgaac gttacggttt tcaaccattg ggacatgaat
     4261 atgatggtga gcaagtatat cgtttgttcg tttaagtatt acgaagtaaa gatgtgaaat
     4321 ggaaaatgta attaaatgac agggaggatt aacaattgaa aaataaattt catcatattg
     4381 tacgagctgt tatgttaaag gataataaat tattattggc tgaatatatt ggccatcatt
     4441 attttttacc aggtggtcat gttgaaggtg gcgaatcagc tgagaaagca ttaataagag
     4501 aactaagaga agaactcgga gtaaattgta gtataaaaca atttttagga gtgatagaaa
     4561 accaatggca agaccatgaa gttcttcatc atgaaatcaa tcatattttt gaagtagatt
     4621 cgcaagagtt acatattgat ttcataccaa aatctaaaga atctcattta gcgtttcact
     4681 ggatagatta taatcaagag gctttaaata cgtataaaat catgccagta ccttcagtta
     4741 aagagttact agaaaggaaa ttaagcgatg aactactaaa ctgttggatt agcaattttt
     4801 aaacacctct attaatagta aatacgtcca ctgaaaggag attaagaaaa taggctaaaa
     4861 aacaaggtac atcacctagt gtaagttcat attttgttga tgtacgcatt tagtctatgg
     4921 cttttatcta cttgttttgc aaaaatatat aaactgtaac attgggacgg aatttatttt
     4981 tataaatgga gggtttcgta atgaatcagt ttcaacaaga actacaatca ttaaacctta
     5041 atgattatca atctggtaat gttgtgtatt gggatcaact acaacaaaat caatatccat
     5101 actattacat tcaagacgat gcacgtcgtt gtggcggatg cggaggttgt ggtggtcgct
     5161 gtggtggatg tggcggcgga agatgtggag gttgtggtgg tcgttgtgta ggctgtgctg
     5221 gatgtttcgg ttgttttaac tgctggaact ggtggatcat ttaagtatag aaaattaaag
     5281 ctattttact tgaaggaaag gtgtgaaaac tattttgcac ttttctttca agctgtaatt
     5341 tttatgcgta ttaaggggga tttatttgta tacatatgat aagttgattt cttgggtaga
     5401 gaatataaaa gaaaaaaatc atagttctgc aacagcactt tgtattataa aagataacaa
     5461 aatcgtacta gagcattata atggctatca ttcaaataca tcgagtaata aaaaagtaac
     5521 cgcatcttca cagttcaacg ttgcttctgc gagaaaaagt tatttaggat taatgatagc
     5581 atatgcgctt tacgagggaa aaataaactc tattgatgac gaggcgataa agtactttaa
     5641 tgatttcgac cctgtattac ttggtaaaac aacgataaga catttagtaa cccattcgca
     5701 cggtttagga gaaacgaatg aggggacaat ttttcgtgaa tttgagcctg gacaagcatg
     5761 ggcgtataga gatattaatg taagaatgat aacacgtctt atttatcagc tatataacaa
     5821 aagttttcct gaattgttaa aggagcgtgt atttgatcta gctaacttca aagaaacagg
     5881 ttggagaatt cagcaagatg aaaacttagt taaagttgtt gataatccaa atgaagacgc
     5941 aattagtgaa attggtacgg tagatgatgg gactgaaaaa aatctgtttg tctcagctag
     6001 agaatttgct tattggggca atcttcattt aaatcaaggt gtgataaatg gtaagaaaat
     6061 tgttccaaaa gaagtgatag aaattgctac gagtttgcag agtccaacat ttacaaacaa
     6121 ggaactacca caaaacgggt tgttttggtt tgttcaaaat gaacctgcgc ttttaagtga
     6181 gcttggagaa cgagttccaa aaggatctta tcaaatatta ggaattacag gaccgactat
     6241 tttagtaata cccgaatata atgtagttgt tgcgaaaatg tataataaaa gatataacta
     6301 cggcggtgat aattatttat attatttacg tgaatttagt aatttagtcg ctgatacatt
     6361 tcgaaacaat aatagggcat aattactcct ataatgaaaa ggagtgattt aaagtgaaag
     6421 atgaagtgag caaaaataaa aagcaaaata taaaagatgt aattacagag caaaatgcga
     6481 tttatagcga tcctaaagat gcagctaata tgcaaactgt accgcagccg aaagattttg
     6541 atgaaatcga atattaattt ttcatccgac tatatgatga atgaaaaata cgtcttaagt
     6601 cccatcacat atcagaaatc atttggtaac atatatacat aagcatttga aaatgtctta
     6661 tcttaacaaa gctttcttta gaaatatctg atataacctg caaaaggcac ttctttatga
     6721 agtgtctttt tgtgtatatg aacttaatta ccattttccg tacagtatct tgcaatttat
     6781 gatggatata ctaactgtga aaattcatgt caagataaaa gaaggggaca gttagtatga
     6841 aacaaacttc tataaatccg ttactaattg ttctaggtac aatcatagtt caaattggcc
     6901 ttggaacaat ttatacatgg agtttattta atcaacccct tgtaagtaag tttggatgga
     6961 atcttaattc agtagcgata actttctcca taacaagttt ttctttatca ttctcaactt
     7021 tatttgcagc aaagttgcag caaaaattag gacttcgtaa actcatcgct actgcaggaa
     7081 ttgttttagg actcggttta atacttagtt cacaagtttc ttccttacca ttactatatt
     7141 tattagctgg tgtcgttgtt ggttatgcag atggaactgc ttatattaca tcactatcta
     7201 atttaattaa atggtttcca aatcgaaaag gacttatttc tggtatatct gtttctgcat
     7261 atggaatggg cagcttaatc tttagatata taaacggaag tcttatcgat agtcttggtg
     7321 tatcacaagc atttttatat tgggggatta tcgtcctact tttagtatta accggatcgt
     7381 tcttcttacg tgaagcaatc gtaagtaaca tcgcaactga gactttacac aatgactata
     7441 cacctcgtga aatgatgcga acgaaacaag tgtatctctt gtttttcatg ttatttacat
     7501 cgtgtatggg agggctatat ttaatcggta tggtaaaaga tattggcgta cagctcgttg
     7561 gacttagtgc agcaactgca gctaacgccg ttgcaatgat tgcaatcttt aatacggtag
     7621 gtagaatcgt tcttggaacg ttatcagata aaattggccg aatgaaaatt gtctctgcaa
     7681 cgtttataat aattggattg tcagtcttta ctttaagttt cattcctcta aattacggaa
     7741 tttatttcgc ttgtgtagca agtgtcgcct tttgctttgg tggtaatatc acaatcttcc
     7801 cagcaattgt tggagatttc ttcggattaa aaaatcatag tacaaattat gggattgtat
     7861 atcaaggttt tggatttggt gcacttgcag gagcattcat cggagcacta cttggtggat
     7921 ttcaaccgac ctttattata attggtgttc tatctgttat ctctttcgtt atttcaatat
     7981 taattcgtcc accaaaagca gaaaagaaaa aagaaacaaa acatttatat cggaaagtag
     8041 cttaattctg gaaaagagtc ctaataaagg gctcttttta tattacaaaa tatttgatta
     8101 ccccttttta ataaatgttt aggaagaaaa gagttactta attcaaaatg aaatgatttg
     8161 aaaagtaata aagagattta tttctcaacg aacgaagtag cagattagta aaatattcat
     8221 atcgttttat agaggtactg aaatcatgtt ataaaactac attaatcacc tttgttatta
     8281 gatatgcaat tcatttgtat accattattt tattataaag gtagttggct tatattggtt
     8341 acaaccataa tttcttcttt tatcgcattc atttcaacaa atattgatga catttttata
     8401 cttcttgtat tattttcaca agtaaggaca ggagtaatta aaaaagaagg tagagctgtc
     8461 cgggaaaaaa ctaagatgaa agaactttat attgttattg gacaatattt aggatttagt
     8521 gtaattattt ttttgagcat catgggttct ttaagctctt ttttcatttc tatatcttgg
     8581 attgggttat taggattcgt gccaatttat atgggagtga aaggactatt gtcacttcgt
     8641 tcttataaag gtaatgaagt cattgctaac gcttctagtt cattatttaa agtagcttcg
     8701 attacattag ctaatggagc tgataatatt tcaatttata taccaatgtt cgctagtcaa
     8761 aatcttaaaa caaatattgt tacagtagtt atcttctttt gcatgatagc aatatggtgt
     8821 tttattagtt acacattgtt aagagctcct attttagcta aagtacttga gagaaattgt
     8881 catattatcg ttccaatagt tctaattggc ttaggaatgt tcattctttt tcgcagtaat
     8941 acaattgaat tattgtacta atatgttaaa actaacggaa gttttaatta aaaagaatca
     9001 aatgctgtat aaagccgtac tacagataaa atgtagtacg gcttttcttt attttagtga
     9061 aatgaaacaa taccctacga ctattaagtt agacaggata ttacgattat taaattgaat
     9121 agatacttta aaaggagtgt gataattaaa atgaaaatag taaaacaatg ggtccaagaa
     9181 gatagtgatt acataagaga aaaagtaatt gaatataacc aaaaacatat ttcggaagaa
     9241 gagaaaaagc cttcagaaaa aattagtttt atcgtgagaa atgaaaagga aaaaattgta
     9301 ggtggaataa ctgcaattac attttggcac cacatacatg tcgatttcct ttgggtttct
     9361 gaagagtata gacacgaagg ctacggaagc aagttattaa aacttattga agaatttgca
     9421 attgaaaatg agtgtaggct aatcaattta gatactttta gctttcaagc tcctgatttt
     9481 tataaaaagc atggatataa agtaattgga gtaagtgaag atcatccgaa aggacataat
     9541 cattactatt tagaaaaacg tttataaagt gtatatagaa ttaacgttag tattttgaat
     9601 acaaatttat ccttttggat tatgcattta aaggagaatc ttgagtgaat tcatatttag
     9661 aggcacttct aaagcaaaaa aagatcgaag tgaaatatta gttacgggaa actaattatt
     9721 tatagcgaat atacctaaaa taggcatcta aatatatgca ttcagatgaa ggggaaatgt
     9781 tatgaattca tttgtaaatg ataggtttga tgaaacgaga aaacatctat tcgaggaaat
     9841 tatgatattg gatgatgctc aattcaatag taagccagac aagaataaat ggagtatagc
     9901 acaagtatgt catcacttag ttttattgga taaagtggtc ataaaggtta tttcatctgg
     9961 cttaaaaaag attgatagta ccctaaaaga gcgtcgagaa atccgctcta tattacaaga
    10021 tagatcgtta aaatttatag ctccaggaat gattgaacca agtttagaac catttgaagt
    10081 gcagcaaatg gtcgatttgt taaacgaatc gagaaaagaa ttaatgcgtt tcctaagtac
    10141 aatagaagat gaatctatat taacgaaaaa atcagtgatg catccagctc ttggagaatt
    10201 acttcttgat cagtggatag aactgatcta tttgcatgaa cagcgtcata tagaacaaat
    10261 caaa
//
