LOCUS       NZ_NVOB01000041         7692 bp    DNA     linear   CON 01-APR-2024
DEFINITION  Bacillus toyonensis strain AFS095804
            AFS095804_119_G5_Contig41_140618, whole genome shotgun sequence.
ACCESSION   NZ_NVOB01000041
VERSION     NZ_NVOB01000041.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus toyonensis
  ORGANISM  Bacillus toyonensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 29170)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 29170)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Assembly Cell v. 4.2.83
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 54.85x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002552225.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 01:56:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,087
            CDSs (total)                      :: 6,001
            Genes (coding)                    :: 5,774
            CDSs (with protein)               :: 5,774
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 103 of 227
            Pseudo Genes (incomplete)         :: 136 of 227
            Pseudo Genes (internal stop)      :: 60 of 227
            Pseudo Genes (multiple problems)  :: 61 of 227
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:33:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 21478
            Orig. end                         :: 29170
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NVOB01000041.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..7692
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..7692
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..7692
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5288](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5288)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1972..2598)
                     /locus_tag="CON62_RS27485"
                     /old_locus_tag="CON62_27490"
     CDS             complement(1972..2598)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402086.1"
                     /locus_tag="CON62_RS27485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_27490"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000402084.1"
                     /transl_table=11
                     /translation="MEIELKNISKKYENKQVLDNFNLVVNKGEMMAITGQSGTGKSTLL
                     NIVGLLEEPDSGDVIIQGIENAWKSEKKQIELFRYTIGYLFQNYALIDNETVSKNLDVA
                     LEYVKLPNKDDKKKEVLEKVGLLDKLNSKIYQLSGGEQQRIALARLMLKKNDIILADEP
                     TGSLDEVNRDQVLSILKSLNNEGKTILIVTHDPEVSKICTNVVTL"
     gene            complement(2604..4823)
                     /locus_tag="CON62_RS27490"
                     /old_locus_tag="CON62_27495"
     CDS             complement(2604..4823)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002154969.1"
                     /locus_tag="CON62_RS27490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_27495"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_098009950.1"
                     /transl_table=11
                     /translation="MKKILITLVLVAYAVSSFVFTKHFEKREMVNINFSDKNGIQVVEL
                     NKDERPEDKLRKIEELSKKEKINIYKLSYKPIKTSKKQKVTIYAAIGDETQFKQKYPLE
                     WGEIFTQDHSAASYLSSRDTKDKEQVGKINIFSPELMVEIRPIEAAKDENLRGAYFVDS
                     NNPNVVQKVKEKLNTEIGFKTEESERVGAMNILENQGVDKYFIIVLTIVLLLIFLSFLY
                     YILLNYKSLAIRKLFGYTDRELIFKHLLKESIQIHTLSLMVVIIGQVIYLFFYNGMSKF
                     LDFGSTWIVWQLIFSGISILVSLLPSLMIYHIKILELLKNKKPLKLVQFLNYSSKFIFS
                     IFLIIFSFNLINYYQDLTVQNSNQEQWDTTKNYSFYEYQSNIIGDRTNWEYETGIKSKK
                     LFGITNQKGALLIKPSDGITYKKQLEYNNSLNNTIGDVKEYDPHGGNTLQVNSNYLQDN
                     PVYDLNGKKINIDADYGDYLVILVPNKYRSNEADILKMYKEWYQFTRFIDEDKHKESIG
                     EKIEPHKQVEIKLLYIQDNQKHFLYNPMLEVNNQNYSKGAILIVVNSENMGGDSYLSYL
                     TGRYFFPKVDNPSQPYKELKEDIKIAGLQDIILTTPSLYSIVDDYLFKLKSELQFTLLF
                     VTLLLSIEIIITCFTILNYLERNKFIHAVQRINGFDFYKRHSKICFLLSLFWLIIYLGA
                     LTFKLATYTIISLIIPAYWLVELLIVYFIIRFVEKKKNKDVLKGA"
     misc_feature    4776..4796
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 26.98"
                     /tool="antismash"
     gene            complement(5001..5288)
                     /locus_tag="CON62_RS27495"
                     /old_locus_tag="CON62_27500"
     CDS             complement(5001..5288)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768886.1"
                     /locus_tag="CON62_RS27495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_27500"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000768886.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 4.5e-18,
                     bitscore: 56.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLKKTVSSLVLSAAILGSGATAAFAATQNVGGGTWNYGTGGGSV
                     WSNYYHGSKVHKSSVIGQYYYSSPWTNPGYTSYASAPDTAGVDESYWSVK"
     misc_feature    5540..5553
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.97"
                     /tool="antismash"
     gene            5942..6076
                     /locus_tag="CON62_RS32860"
     CDS             5942..6076
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653179.1"
                     /locus_tag="CON62_RS32860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_000653177.1"
                     /transl_table=11
                     /translation="MKDIYELLQEIHINLTEFEEIKCSEVEKEKVKGFVKELIKKKED"
     gene            complement(<6282..>6347)
                     /locus_tag="CON62_RS33040"
                     /pseudo=""
     CDS             complement(<6282..6347)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259872.1"
                     /locus_tag="CON62_RS33040"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /product="IS6 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSIKDS"
     gene            complement(6378..6665)
                     /locus_tag="CON62_RS27500"
                     /old_locus_tag="CON62_27505"
     CDS             complement(6378..6665)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1058: ArsR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016107136.1"
                     /locus_tag="CON62_RS27500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_27505"
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000935902.1"
                     /transl_table=11
                     /translation="MLNYEKDAEVFKALAHPVRIEILKELIIRGACNVTQIVGILGIPQ
                     STVSQHLAKMKSQKLIRNDRKGLEVYYSAQNKTINSIVEILLDYKSTNCK"
     misc_feature    complement(7044..7058)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.82"
                     /tool="antismash"
     gene            complement(7203..7514)
                     /locus_tag="CON62_RS27505"
                     /old_locus_tag="CON62_27510"
     CDS             complement(7203..7514)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637520.1"
                     /locus_tag="CON62_RS27505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_27510"
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="WP_000637486.1"
                     /transl_table=11
                     /translation="MIVTTTNTIQGKEIIEYIDIVNGEAIMGANIVRDIFASVRDVVGG
                     RSGAYESKLKEARDIAMEEMKQLATQKGANAIVGIDVDYEVVRDGMLMVAVSGTAVRV"
ORIGIN
        1 ggaataacca gtacctcgat ttatccaact gttctttatc acgtttccag aggaatcttt
       61 tgcactatat ttagtagggt ccttttcatt agatataatc tctattttgt cataattttg
      121 tttctcattt tctttacctg gataccactc tattaaccaa ttgttaattt cattgttgct
      181 tttatcagag aaacgaattt cagatttgaa actccaatca gtaccaaatt gagaagtata
      241 ttgattacca gccaaactta ccccattttt gttattcaaa cggaattgaa atttcccatc
      301 gttagaattt cctatctgaa caagttcaaa cacttcatca tcgccaagcg aatcggaata
      361 ttgaagtccg tttccttctt ttcctgaaag atattttgaa cctgtcttta cgattacttt
      421 attcgttcct tttttataag ggataatttg ataaccttta ccgtcattta gtgtaatgaa
      481 tggcagtgcc atagatgtct ctaggacgtt agtacgctcc gccaatacat cagataatcg
      541 atcataaaaa tctgcacgag ctttaggatc attattaaca tagttgtcaa ggacatcttt
      601 agatactggc tttgaagtta atgttatcaa tcgtttttgt tcatctgata atttatttgc
      661 tatctgatca aataggtaat ctgaatttct agcaatcacc gtccaatcgc caaaggagtc
      721 accggaatat tttttgtaaa gtggaccaag aaatcttggg tttttaccag atgctttttc
      781 tacaaatgca tgttggcctt gatttatcac aactccggtc tttccttcgt ctaattgtgc
      841 ttgtgtaatg taaagaagat cgtgttctga aacatttgct gcattagggt ttaaatctat
      901 aattgcacct gcttcagtaa ttttaagctg ctgtccagat tttagttgat gaatttcaat
      961 ttttccattt ggatagcgta ctttaacttg tttaagaaaa ttcaaatcac cttgtgtatg
     1021 atacatagaa atattaaatt ccccagattt aaaactagga gcttggtaag tttcgttttc
     1081 tgcatataca tcaatgtgag tacctgggtc tcgatcccct gggcttattc caagtgcttc
     1141 ataagtgttt gctacagcac ctaatacagt aatagttata tctactaccg cacctatccc
     1201 tgcactagcc ttactttctc caacaggagc catagaaaaa agaataccta atgccgctgt
     1261 tgcagggatt accttctttc caattcctac aggaagaatt ttttttaccc tttttgtttc
     1321 ttttccattc ataatgtcat tcctcgcttt ttatttttct atttattaat ccgaatctta
     1381 cgttcgaaag gttatcttaa tttaaaaacc tttcaaatct cttacgtaaa tattacaaaa
     1441 gtctttcata aggtgatttt attgtttgtt ttcactaatt ctccataatt cactcttaac
     1501 caaagtaata ttcctataac atatcccttg aaacctgttt gaactaccct cactttcact
     1561 tcgtttagaa nnagattgat acttgcgtta attcaaatag ttgcacaaaa acgaaacctt
     1621 tcaaactcga atttaaaggc catctaccag aaaaggctaa agcctcagtt aaaatgaagt
     1681 tggatactgg agaataaagc cactaaaaaa agaatgccct ttttgttatt taaaggagca
     1741 ttcttttttt ataagataag cgtacattcc ctttcagcaa aggtttaaag aatttcttgt
     1801 tatgaggatc tttatgtttc tcatgtatga cgaaagcctg ttacaataga actaagtcaa
     1861 atatgaaata cccttaggaa gctctcatag gtataagatc tatgggggat ttttcattac
     1921 agttattttg aagtttttgc accataaccg ttaatacctt attttcaaaa tttatagtgt
     1981 aacaacgtta gtacatattt tagatacttc agggtcatga gtaacaatta gaattgtttt
     2041 tccttcatta tttaatgatt ttaatatact aagcacctga tcccgattca cttcatctaa
     2101 tgatccagtt ggctcatctg ctaggatgat atcattcttt tttaacatga gcctagctaa
     2161 tgctattctt tgttgttctc caccactaag ttgataaatt ttactgttta atttgtctag
     2221 tagcccaact ttttctagaa cttctttttt cttgtcatct ttattaggta acttaacata
     2281 ttctagtgct acatctaaat ttttgctcac agtctcatta tcaattaatg cataattttg
     2341 aaagagatag cctattgtat atctaaaaag ctcgatttgt tttttctcgc tcttccaagc
     2401 gttttctatt ccttgtatga ttacatctcc agagtctggt tcttctaaaa gacctactat
     2461 atttaatagc gttgattttc cggttccact ttgacccgta atagccatca tttctccttt
     2521 atttacaacc aaattaaaat tatcaagcac ttgtttattt tcatatttct tagatatatt
     2581 ttttagctca atttccactt gaactacgct ccttttaata cgtccttatt tttcttcttt
     2641 tctacaaatc gaatgatgaa ataaacaatt aataattcaa ctaaccaata agctggaatt
     2701 ataagtgaaa taattgtata ggttgctaat ttaaatgtta atgcacctaa ataaattatt
     2761 aaccaaaaaa gacttaagag aaaacagatt ttactgtgtc gcttataaaa atcaaagcca
     2821 ttgattcttt gaacggcatg aataaattta tttctttcta aataatttaa aattgtaaaa
     2881 caagtaataa taatctctat gctaagtaat aatgttacaa aaagaagagt aaattgaagt
     2941 tcgcttttta atttaaagag ataatcatct acaatactat ataatgacgg cgttgtaaga
     3001 atgatatctt ggagacctgc gattttaatg tcttctttta attctttata aggctgactt
     3061 ggattatcta cttttgggaa aaaataacgc ccagttaaat aacttaaata gctgtctcca
     3121 cccatatttt ctgagttaac aacaatcaat atagcgccct tgctataatt ttggttgtta
     3181 acctctagca taggattata caaaaaatgt ttttggttat cttggatata gagaagttta
     3241 atttcaacct gtttatgtgg ttcaattttt tctcctatgc tttccttatg tttatcttca
     3301 tcaataaacc ttgtgaattg ataccattct ttatacattt ttaaaatatc agcttcgttg
     3361 ctgcgatatt tattgggaac taatataact aaatagtcac cataatcagc atcgatattg
     3421 atttttttac cattcaaatc ataaactgga ttatcttgta aataattaga gttcacttga
     3481 agggtgttac cgccgtgagg atcatactct ttaacatctc ctatagtatt gtttaaagaa
     3541 ttgttatact ctaactgttt tttgtacgtt ataccatctg atggttttat aagcaaagcc
     3601 cctttttggt tagttattcc aaatagtttc ttgcttttaa ttccagtttc atattcccag
     3661 tttgtgcggt cgcctataat attactttga tattcataaa aactataatt ttttgttgta
     3721 tcccattgtt cctggttaga gttttgtaca gttagatctt gataataatt tataagatta
     3781 aaagaaaaga taattagaaa aatcgaaaag ataaactttg aactataatt taaaaattga
     3841 actagtttta atggtttttt attttttaat agttccagaa tttttatatg atatatcatt
     3901 aaagaaggaa gtaggctaac tagaatagaa atacctgaaa aaattaattg ccacacaatc
     3961 catgtactac caaaatctaa gaatttactc atcccattat aaaaaaacaa gtatattact
     4021 tgtcctataa taactaccat taaggataac gtatgaattt gaatactttc ttttaataga
     4081 tgcttaaaaa tcagttctct atccgtatat ccaaacagtt ttctaattgc caaactttta
     4141 taatttaata atatataata taaaaaactc aggaagatga gtaacagaac tatagtaaga
     4201 actataataa agtacttatc aactccctga ttttctagga tgttcatagc acctactcgt
     4261 tctgactctt ccgttttgaa cccgatttcg gtatttaatt tctcttttac cttttgaaca
     4321 acgtttggat tgttactatc aacaaaataa gcgcctctca agttttcatc ttttgccgct
     4381 tctataggcc taatttccac cataagttca ggtgaaaata tgtttatctt tccaacctgc
     4441 tctttatctt tcgtatctct tgatgacaaa taacttgctg cggaatgatc ttgagtgaat
     4501 atctctcccc attctagcgg atatttttgt ttgaattgtg tttcatcccc gatagctgca
     4561 tagatagtta ctttttgttt ttttgaagtt ttaatcggtt tatatgataa tttatagatg
     4621 ttgatttttt cctttttgga taattcctca atctttctta gtttatcttc cgggcgttca
     4681 tctttattca attcaaccac ttgaattcca tttttatcag aaaaatttat atttaccatt
     4741 tctctttttt caaagtgctt agtaaataca aatgaagata cagcataggc tacaagtact
     4801 aaagtaatta atattttttt cataccagtc tccattttcc gtattctatt ttcatttaga
     4861 attcaaatga agatagaata ccctttgtct tcaaatatta ataaaaataa tttgcttatc
     4921 tatatgtata taagtcacat aaaatagaat cctagaacat gcaggtaata acccacgagt
     4981 tctaggattt ttcttacttt ttatttaaca gaccaataag attcatctac accagctgta
     5041 tcaggtgcac ttgcatatga agtataacct gggttcgtcc atggagaact ataataatat
     5101 tgtccgatta ctgaagattt gtgaactttg cttccgtggt aataatttga ccaaactgaa
     5161 ccaccaccgg ttccataatt ccatgttccc ccccctacat tttgtgttgc agcaaatgct
     5221 gctgttgctc ctgaaccaag tattgcagca cttaatacta atgaacttac agtttttttc
     5281 aatttcaatg ttttttcccc ctttaacatc taattaattt atttagatat tgcagactga
     5341 ctaccttttt atttttaaac aaccgcatta aaaaataaat atatagaaca gaatacaata
     5401 aactaacaaa taggaagtaa ataaggtaaa gatactcaaa tgaaaaaata attcaattaa
     5461 aaatagggtg ggaaatatta acttcaaatc gtttttttaa atattatcac agtttttttc
     5521 aagaaagaca cattcaaaat tttttaaata aaattcagaa ttttctgtaa ttatttgata
     5581 taaaaatagt gatacttaaa aagaaaattt tcaatcaagt aagtatttac aaatatagag
     5641 gggagtaatt ggaaagtatt ttaaatgatg caatttaaga aatagacatt ttcaattttt
     5701 tgttagcatg gaatcgtatg gtatgcgatc taaaaaaaat ttgtggtaag tgtgagaaat
     5761 cactttgttt aatacgttat ataaggtata agaaattgtc tatccattat taacgttaca
     5821 tattacaaat taaatatttc gataatacgg aaagaataaa tttttactaa atatgcctta
     5881 gattcacttt cttaattgca taaaaaatta aaataagaaa gaatttaggg ggtgagatag
     5941 tatgaaagat atttatgaac tattacaaga gatccatata aacctaactg aatttgaaga
     6001 aataaaatgt agtgaggtag aaaaagagaa agtaaaaggt tttgtaaaag agttaattaa
     6061 aaagaaggaa gactgaaaat gtttataacg caagtatcaa tctaaagaat gaagcgataa
     6121 ggcttctaac cgcaaggact gtggagatag cctaactctt tacttaggaa tctccctctt
     6181 ctaaaaaagt gaaagtgggg gtagtttaat cataagaagt cattgacgta tttgaaacat
     6241 gaaaattaat tgtattaact ttaaataaca gcacggtatt agtacatcca ttgaccttta
     6301 atttttacat atgtctcatc aatccgctaa gaatccttga ttgacttagg gggttctgga
     6361 acaaagatta ttatttttta cttacaatta gtagatttgt aatctagtag tatttcaaca
     6421 atggaattaa tagttttgtt ttgggcacta tagtagactt ctaatccttt tctatcattt
     6481 ctaattaatt tttgagattt catctttgct aaatgctgtg aaacagtaga ttgtgggata
     6541 cccagtatac ctacaatttg tgttacatta catgcccctc ttataattaa ttctttcaaa
     6601 atttcaattc ttactggatg agctaatgcc ttaaaaactt ctgcgtcttt ttcataattt
     6661 aacaaactaa acatctcctt ttgaatcgat atatgtttat aatattaata atttattctg
     6721 catcatcttt ttgatttaat tagtttttta ttttttcaac ataccctggt atgcttgaga
     6781 cactcttatt ctcttttttt acttccttta attcatctga aaatgttgca tttggctttt
     6841 tcatattaga cataattcca agatcacctt tgatggtttc ttacctaagt aactttgctt
     6901 aaccgaatca gtggtttcta gtagtaggaa ttgacctcta ccaaggaatt gactcccttc
     6961 atgtactttc gcaagaatcc tttttgggat atgccagaaa ttccaatagt taatagcgat
     7021 tgtgtaaacc ctacacctaa tgtatcgact tatttgatac attgttagct attcgccaga
     7081 acctctatgt aactaatttt tttcaaagag tatttttcta taaaaaactg caactctaat
     7141 tgttagttat gtctaacaat tagaattgca gttcaccaat taggttatat tttgctttct
     7201 atttagacac gtacagctgt accgcttact gcaaccatta gcattccatc acgaacgact
     7261 tcgtagtcta catcgatacc aacaattgca tttgctcctt tttgcgttgc aagttgtttc
     7321 atttcttcca tggcaatgtc acgagcttct tttaatttac tttcataagc accagaacgg
     7381 ccacctacaa cgtcacgaac agaagcgaat atgtcgcgta cgatatttgc acccataatt
     7441 gcttcaccgt ttacgatgtc tatatactca ataatttctt taccctgaat agtattagtt
     7501 gttgttacga tcattattaa cacactccta taatttttaa aaagttattg ttgattgcta
     7561 taatcctatt attaatttta aaaactaaat aaactatcta ataaccttac ttgaactacc
     7621 cccactttca cttcgtttag aagaggggga ttcctaagta aagagttcta ttgaactcta
     7681 atttattagg ct
//
