LOCUS       NZ_NVOB01000040        10324 bp    DNA     linear   CON 01-APR-2024
DEFINITION  Bacillus toyonensis strain AFS095804
            AFS095804_119_G5_Contig40_140618, whole genome shotgun sequence.
ACCESSION   NZ_NVOB01000040
VERSION     NZ_NVOB01000040.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus toyonensis
  ORGANISM  Bacillus toyonensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 127335)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 127335)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Assembly Cell v. 4.2.83
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 54.85x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002552225.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 01:56:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,087
            CDSs (total)                      :: 6,001
            Genes (coding)                    :: 5,774
            CDSs (with protein)               :: 5,774
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 103 of 227
            Pseudo Genes (incomplete)         :: 136 of 227
            Pseudo Genes (internal stop)      :: 60 of 227
            Pseudo Genes (multiple problems)  :: 61 of 227
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:33:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1822
            Orig. end                         :: 12146
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NVOB01000040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10324
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10324
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10324
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5324](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5324)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(342..503)
                     /locus_tag="CON62_RS32220"
                     /old_locus_tag="CON62_26765"
     CDS             complement(342..503)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866154.1"
                     /locus_tag="CON62_RS32220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_26765"
                     /product="hypothetical protein"
                     /protein_id="WP_000866207.1"
                     /transl_table=11
                     /translation="MKVLKNQLREWKNQSKKKNKKKRKEKFSTREIEDLMGVHRPCYER
                     RRGALRQK"
     gene            complement(786..1586)
                     /locus_tag="CON62_RS26770"
                     /old_locus_tag="CON62_26775"
     CDS             complement(786..1586)
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097507.1"
                     /locus_tag="CON62_RS26770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_26775"
                     /product="C1 family peptidase"
                     /protein_id="WP_000097507.1"
                     /transl_table=11
                     /translation="MSSNESNFSFTSNKDIVKYLNVRKDPVDLRDKIFRSYLFSNEADL
                     PTQVDLRPKMSGIVNQWNIGSCTANAIVSGLREYLLLQSGRPFTCLSRLFLYYHEREIE
                     GHIQEDSGANVRDGMDVLKKIGVCPEEDFPYTENFRDKPSPKAESDATLYKIGTYHRIQ
                     DLSGLKAALAHGLVVVLGFAVPKSFMSEEVKKTGIVPVSGTDDVIVEDGQQAGHAVCAA
                     GYDDNKKTVIIRNSWGEEWGDKGYCYFPYELFENGIVQDMWTGR"
     gene            2080..2301
                     /locus_tag="CON62_RS26775"
                     /old_locus_tag="CON62_26780"
     CDS             2080..2301
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807684.1"
                     /locus_tag="CON62_RS26775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_26780"
                     /product="hypothetical protein"
                     /protein_id="WP_000807684.1"
                     /transl_table=11
                     /translation="MAEHTKCVCEQLERLQLGTEVDVFLTGTTLEDLIFTNFNPDNFCV
                     TFVDNTTEPGSIIVLDCRDIQALRIEAE"
     gene            2382..2681
                     /locus_tag="CON62_RS26780"
                     /old_locus_tag="CON62_26785"
     CDS             2382..2681
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110270.1"
                     /locus_tag="CON62_RS26780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_26785"
                     /product="hypothetical protein"
                     /protein_id="WP_000110270.1"
                     /transl_table=11
                     /translation="MSVFINYAITPKVFNPNLTSLPNNRRSFIPTQKESSKNICYLLET
                     YYLGKKIKQLLVNGTILKASIFVGFHNRSRLATFIDENGDITVVNCERIDAILL"
     misc_feature    complement(2781..2799)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.44"
                     /tool="antismash"
     misc_feature    complement(3648..3664)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: medium, score: 22.69"
                     /tool="antismash"
     misc_feature    3648..3664
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.06"
                     /tool="antismash"
     gene            3788..4135
                     /locus_tag="CON62_RS26785"
                     /old_locus_tag="CON62_26790"
     CDS             3788..4135
                     /GO_component="GO:0043592 - exosporium [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343262.1"
                     /locus_tag="CON62_RS26785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_26790"
                     /product="exosporium leader peptide-containing protein"
                     /protein_id="WP_098009930.1"
                     /transl_table=11
                     /translation="MDEFLSSAALNQNSIGPTLPLMQPFQFPTRPTGNTGPPGIVSVTF
                     NQSHIPATSPTNLVLPAISVGVNQIVKLEGVFRFDYVSLSPLTEFTVSSILALKRGVFT
                     IAVQMELQEDI"
     gene            4573..4845
                     /locus_tag="CON62_RS26790"
                     /old_locus_tag="CON62_26795"
     CDS             4573..4845
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088127.1"
                     /locus_tag="CON62_RS26790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_26795"
                     /product="hypothetical protein"
                     /protein_id="WP_000088127.1"
                     /transl_table=11
                     /translation="MSRKSDITAKDRKDAGKKALKSAVVKGWINVGKWGFFGDMVAFMD
                     RYYTGYKNYNLKKKRERRADSSSSFWFWCNKSAYKSQRVRQEYKT"
     gene            complement(5001..5324)
                     /locus_tag="CON62_RS26795"
                     /old_locus_tag="CON62_26800"
     CDS             complement(5001..5324)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108508.1"
                     /locus_tag="CON62_RS26795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_26800"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001032074.1"
                     /sec_met_domain="TIGR03601 (E-value: 2.1e-38, bitscore:
                     123.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKFQQELQALSLNDYQSGNIVYWDQQNQYPYYYFEDAARRCGGC
                     GRCGGCGGRCGGGGFRCGGFRCIGCFGCFGCGGCGGCGGCSNCFNGFNGTTDTTGTIVT
                     YEY"
     gene            complement(5975..6664)
                     /gene="tenA"
                     /locus_tag="CON62_RS26800"
                     /old_locus_tag="CON62_26805"
     CDS             complement(5975..6664)
                     /EC_number="3.5.99.2"
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="CON62_RS26800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_26805"
                     /product="thiaminase II"
                     /protein_id="WP_000144532.1"
                     /transl_table=11
                     /translation="MTFSQTLRKEVDSIWEASFHHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITEEEKGHFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYKEWISAY
                     GSDWFRTLVEEQITRLDTIAEQVTESDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDT
                     EVKDVIG"
     regulatory      complement(6792..6897)
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            complement(6971..7885)
                     /gene="ddlB"
                     /locus_tag="CON62_RS26805"
                     /old_locus_tag="CON62_26810"
     CDS             complement(6971..7885)
                     /EC_number="6.3.2.4"
                     /codon_start=1
                     /gene="ddlB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="CON62_RS26805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_26810"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_002106271.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 2.5e-23,
                     bitscore: 75.6, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 2.5e-15, bitscore:
                     49.8, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 2e-09, bitscore: 30.2,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 1.9e-52,
                     bitscore: 170.6, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 2.1e-07, bitscore:
                     23.6, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSGICMDKNISKKILRY
                     EGVETPDWIELTKMADLKLDDLDKLGFPLVVKPNSGGSSVGVKIVNDKNELISMLETVF
                     EWDSEVVIEKYIKGDEITCSIFAGKHLPIVSIRHAAEFFDYNAKYDDVSTIEEVIELPT
                     EIKERVNKASLACYKALKCSVYARVDMMVKDGIPYVMEVNTLPGMTQSSLLPKSADAAG
                     INYSKLLDMIIETSLRVRKEEGF"
     gene            complement(7890..9338)
                     /locus_tag="CON62_RS26810"
                     /old_locus_tag="CON62_26815"
     CDS             complement(7890..9338)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-465). E-value:
                     1.2e-29. Score: 95.4. Matches aSDomain:
                     nrpspksdomains_CON62_RS26810_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="CON62_RS26810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_26815"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_000471599.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 2.6e-29, bitscore:
                     95.3, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYFKKMIMKGHLLGDQKIPSTRELSEL
                     LSVSRNTVLSAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKNKLNTVTTLADE
                     LDLMKHGVRWEKGMVVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYRPL
                     MKYLLHYMEIKGVDISNKDILITNGFTEGLDIVLSSLAKKSGRVICENPTHHAALKLFR
                     LHGLEVHGIDMNDDGIDTTEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYKVPIIEDGFNEELRYSGSHLSPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQY
                     IPFKRMTGDGGLHLFIELEEKIHARTLLQKCYEKGVTFSPGDVFYSDGGGANTFRLGFS
                     RLKEKEIVSGIQIIGETLKNETWS"
     aSDomain        complement(7944..8903)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CON62_RS26810_Aminotran_1_2.1"
                     /evalue="1.20E-29"
                     /label="CON62_RS26810_Aminotran_1_2.1"
                     /locus_tag="CON62_RS26810"
                     /protein_end="465"
                     /protein_start="145"
                     /score="95.4"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYRPLMKYLLHYMEIKGVDISNKDILITNGFT
                     EGLDIVLSSLAKKSGRVICENPTHHAALKLFRLHGLEVHGIDMNDDGIDTTEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYKVPIIEDGFNEELRYSGSHLSPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQYIPFKRMTGDGGLHLFIELEEKIHARTL
                     LQKCYEKGVTFSPGDVFYSDGGGANTFRLGFSRLKEKEI"
     misc_feature    10255..10268
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.92"
                     /tool="antismash"
ORIGIN
        1 ataatatctc tttgaatgct tcctgcacgt ccgttcccta atcgatttag aaactgatca
       61 atacgtactg acattctttc aagccactct tctcgtttgc tttgttgaat atttgctata
      121 gcaacatctt ctaacagctt accaactgta tgtgtaggac cgtgctcacg tatttcataa
      181 gaaggagtga ctttcatttc tttacgcatc atcccaaatt gtctatgtat acgtacactt
      241 tccaacacac cttctaattc ctcttgtgtc gctgttctat caatttttgg taagaaagat
      301 aattgtttag tcatgtaaga ccactccttt ttatttttaa attacttttg tcttaatgct
      361 ccacgtctgc gttcataaca aggtctatgc acccccatca aatcctcaat ttcacgagta
      421 ctaaatttct cttttcgttt tttcttattt ttctttttcg attggttttt ccattcacgt
      481 agctgattct ttaacacctt catttcccca tctccctttc caaaataaaa aaaaggacac
      541 ctattcctaa agcagcttga attgctgctt taatgaattg gtgtcctcta gttttctagc
      601 cggactatat ttgtttttcg ttcacttaaa ataccagctt gtataaaaat attactcata
      661 tctttttccg ttgtaggtaa caaacctgac tccctttatt tccacatctt tttcaagctc
      721 ttttatacta cccgtaacat gtataacgtg ttttattgtt tccatacttc cacttcttta
      781 tctaattaac gacctgtcca catatcttgt acaattccat tttcaaataa ttcatatggg
      841 aaataacaat atcctttatc tccccactct tctccccagc tgtttcgaat aataacagtt
      901 ttcttattat catcatatcc agcagcacaa acagcatgtc ctgcctgttg accatcttca
      961 acaattacat catctgttcc tgataccgga actataccag tctttttaac ttcttctgac
     1021 ataaaagatt ttggcacagc aaatccaagt acaacaacta atccatgggc aagtgcagcc
     1081 tttaatcccg ataaatcttg aatacgatgg taggttccaa ttttataaag cgttgcatct
     1141 gattcagcct ttggtgatgg cttatctcta aaattctcag tataaggaaa atcctcttca
     1201 ggacataccc caattttttt aagaacatcc ataccatcac gaacattcgc tccgctatct
     1261 tcctgaatat gtccttctat ttctcgctca tgataatata aaaacaaacg agataagcaa
     1321 gtaaatggac gacctgattg taataatagg tattctctta accctgaaac aattgcattc
     1381 gccgtacaag aacctatatt ccattggttt actatacctg acattttagg acgaagatcg
     1441 acttgcgttg gcaaatccgc ctcattacta aacaagtaac tccgaaatat tttgtctctt
     1501 aaatcaacag ggtcttttct tacattaagg tactttacaa tatctttgtt actggtaaaa
     1561 ctgaaatttg attcatttga gctcattgca aaaatccatc ctttctttat atctagatga
     1621 ttcatttctt ttctatactt aacttaaaaa ttaaaattat acctttttaa ttttaatttc
     1681 atttgcattc actacatcca aatttttaat tatttatctt tttaattatc aatttctatt
     1741 ttcaatttat ctcacaacca cttgaactct atattttttt tcttctttat ttgtttcgat
     1801 tactttttct attcacttaa ttgatctctt aacacattca tttccccatc tcccttttca
     1861 aaataaaaag gacacctatt cctaaaacag ctttaatggc tggctgcttt aatgaattgg
     1921 tgtcctctag ttttctagcc ggacgatatt catttcaaag gattatttta taaaaatatt
     1981 aactagcaaa ataattaata ttactaagct attactccaa tcgttctatt taatttatac
     2041 atatattaat agtaatttga gaaaaggagg tgtttttata tggctgagca tacaaaatgt
     2101 gtttgcgaac aattagaaag attacagtta ggtacagaag tagatgtttt ccttacagga
     2161 acaacactag aggatcttat ttttactaac tttaatccag ataatttttg tgtaacattt
     2221 gtggataaca caacagaacc tggctctata attgttttgg actgtcgtga tattcaagcg
     2281 cttagaattg aagctgagta gagtaaagat ggggctttga aaaaagccct atttcacaca
     2341 gcatatttca atataaatta taaaatagag gagccggaaa aatgtcagtt ttcatcaact
     2401 atgctattac tcccaaagta ttcaatccta atctaacatc tcttccaaat aaccgacgtt
     2461 cttttatccc aacccaaaaa gagagttcta aaaatatttg ctatctttta gaaacatatt
     2521 acttaggaaa aaaaataaaa cagctgttag ttaacggcac aatattaaaa gcttctatat
     2581 ttgtaggatt ccataataga tcccgccttg ctacatttat agatgaaaac ggagatatca
     2641 ctgtagttaa ctgcgagcgt attgatgcga ttctactcta attatgaatt tacatttaaa
     2701 taaataatca ttattttcaa gatacgcgtt agttcttaca cataatacca ataactgtta
     2761 ccataaaaaa cttggtccaa agtgcactta tatacagtac cttttttatt tgaccgaata
     2821 agcctccaga ttccgtcaat aatgtagaca acccattaag ttactttctc cttgttcccc
     2881 cttggagaac cgaacagtta gcttttgcta gatgctcttt gttagtaaaa acgcactcaa
     2941 ttgactggat acacatataa tattttgtgc ttctctcttt ataaaatgaa gttttaattt
     3001 agttttcttt cctgcataat attttggtat ttgtttatag catagatgta actatttgtt
     3061 acaacatata tctgtatcca gtgaacttct aaaattgtac aataaagcaa ttagctactt
     3121 cagctagctg ctttgttgtg caaaataaag gttttatttc tcaacaacca ttattggaat
     3181 taaaacccca ctaatggtaa tatgaaaata cctttcagtc ataattttta acatgtcaag
     3241 tgttgttccc ttttaaaagg tcctgtgtca atcagggcct tttaaacttg ttccttacta
     3301 aaatagcgtt tttattaaaa acttctcaca tttaaaccgg acaagcatat gttattctat
     3361 cgaggctttc cattcatagc attctacctt tcttatttga gagcacgctt atatgtgtgc
     3421 tctttttatt taagataaaa taaggatttt gtttaaaaac cgcacattta ttaagaacat
     3481 acataaaata tcatgcggta ttttttttaa tattagtttt ggttaaagag cgccttaaaa
     3541 gcgctcttta tatttgttaa taaataacca tttttctcaa acacactcac ggtttgtata
     3601 agccaatcat atatttacat agacaagaaa tagcttttta ttatacatgc gcaatttttg
     3661 tgcatgtttt ttctttaata aaataactat tttgtttaat ttttaacata caaccgcttg
     3721 tccatgtcac tttgctcaac gcctacattt actatcagta atataaattt tttagaggtg
     3781 aattatattg gacgagtttt tatcttctgc tgcattaaac cagaattcga ttggaccaac
     3841 actcccactt atgcaacctt ttcaattccc tactagaccg accggtaaca ccggaccacc
     3901 tggcattgtt tcagttacat ttaaccaaag tcatattcct gcaactagcc caactaacct
     3961 cgtattacca gctattagtg taggcgttaa tcaaatagta aagttagaag gagttttcag
     4021 atttgattat gtttccctaa gtccccttac agaatttact gtatcttcaa ttctcgctct
     4081 aaaaagaggc gtctttacta ttgcggttca gatggagctt caagaagata tttaaaaacc
     4141 agtaaatgtt tcaacaaatt taacacacaa cgtttcaata gtctggatag atacgcctcc
     4201 ccctggtact tatgtataca ggctatctct gaatgccacg ggaattggta catccactaa
     4261 cactattagt gaaagaaaca taactgctac agtaataaac atttaaagtg taattcccgg
     4321 tctataaaac gtaatatgaa gtaaaacaaa aacagcttgt agaaacaaag caccctattc
     4381 catgttctaa attttgaata ctagagcagt gcattttata caaacagtta cgtagttttg
     4441 ttacgtttca aaattaatca atagtaataa aaatagatta ttaaatttgt taatcccaat
     4501 aaatagcttt taaagttgcg ctcacttcca ccattcatac tgattttcac atatttcata
     4561 taaagtatga gaatgagcag aaaatcagat ataacagcaa aagatcgtaa agatgcgggg
     4621 aaaaaagcat taaaatcggc agttgttaaa ggttggataa atgtaggtaa atggggattc
     4681 tttggtgaca tggttgcatt tatggacaga tattatacag gctataagaa ttacaatctt
     4741 aaaaagaaaa gagaaagaag ggctgatagc agctcttctt tttggttctg gtgcaataaa
     4801 tctgcttata aaagtcaaag agtgcgtcaa gagtataaaa cataaaaaga cattcgattt
     4861 ttgatttgaa ataaagtcac aatgtttata aaaggttgga taaattatct ctctaaatta
     4921 aaagatgcta cgtgtgtttt gatttatctc ttttataaaa ttgtataacg ctttacaaag
     4981 tagattcctt ttctctctta tcaatattca tatgttacaa tagtaccagt agtatcagtg
     5041 gtaccattaa aaccattaaa acaattagaa caaccaccac agccgccaca gccgccacaa
     5101 ccaaaacaac cgaagcaacc aatacaacgg aatccaccac aacggaatcc accaccacca
     5161 caacgacctc cacaaccgcc acaacgaccg caaccgccac agcgacgagc agcatcttca
     5221 aaatagtagt atggatattg gttttgctgg tcccaataca caatatttcc agactggtaa
     5281 tcattaaggc ttaacgcttg tagttcttgt tgaaacttat tcattttcat gacctccgtt
     5341 tataaacttt aacctcatcg atgctttcca atatttctgt tcgttaaatc taagtagaaa
     5401 taacgctata aactaagttc aatacgtaca cctataaagt atgacctatc actagacggt
     5461 gcaccttgtt catataccta tttttactat gggctcattt ttataaatag aaattataaa
     5521 atgtgaatgt ttgcgtcata tcttcatgct tactaagttc aattcatttc tcaataacca
     5581 tgatgccgta tcctacgtat tcccgtatgc cgcttagatt tttctgtact gtaaatgcct
     5641 ctcgttataa cacttccata tactttctga gagcaccttc agatattcct aactttttta
     5701 tatacttcac tagccgaata atcgatttag tccgtcgtag cgtcaaaaca cctactagtg
     5761 acaggactca atgacggtta agaaattcct acaaaaaaag ccctgattag ggcttttcat
     5821 tctacttttt cagcaagctc tccatgaaat tgctaattga agaaaattga aaaaaagacg
     5881 cacttagatt gatacgtctt ttttgcgata cccctttttt gaaatcatat aaagaggtat
     5941 tacccataca tatgtataac aatacctcta ctctttatcc aattacatct tttacttctg
     6001 tatccactgg ccatttttct aatgtataag ccatttccca aaatgaatat tcatattgac
     6061 tactaataat gaaatgctgt ttcatacgct tccgatcaga ctctgtcact tgttcagcga
     6121 tagtatctaa tcgtgtaatt tgctcttcaa ctaaagtacg gaaccaatca gatccataag
     6181 cagaaatcca ctctttataa atcggctctt ctggctgaca ttcttttaaa cgttcaccaa
     6241 tttcataata tagccagtag caaggtaaaa ttgctgcgat aatatcccct aaatgccctt
     6301 catacgccgc acggtacata tgtgaagtat acgcatatgc agttggggca gggataaaat
     6361 gacctttttc ttcttccgta attcctaatt tctttgcgaa attctcatgt aatgataatt
     6421 ctgcctcgta tgtattttga gcatggtgtg ccatgcgagc tgtcgtttct aattcaagag
     6481 cctttgcagc tcctaaagtt tgtactctag caaaatgact taaataatat gaatcttgaa
     6541 gtacgtaata acgaaaactt gccaaatcta acgtaccttc accaagtttt tttacaaaag
     6601 gatgatgaaa acttgcctcc caaatagaat ctacttcttt acgtaatgtt tgtgaaaaag
     6661 tcatttaaat tcctccaata atgtataaaa tatatgtatt tatccacagt tattaaatct
     6721 gtaaaacaaa aaaaactttc tttacaggcg taaagaaagt gaaatagaca atattaatgt
     6781 attctttaaa tataatcgtc ttcccacttc cctacgctgg tattaaccag atcaggtact
     6841 aagagtctca aaattatgtt tgatctcagc ctacaaaagg cccccctagt ggataaatat
     6901 ttagttgtcg tattgtaatt atcatagcat acttcgtgga actatcaaaa gttttaattg
     6961 tataattatc ttaaaaacct tcttctttcc tcactcttaa tgaagtttca attatcatat
     7021 ctaatagttt actataatta atccccgcgg catctgcact ttttggcagt aaacttgatt
     7081 gggtcatccc tggtaatgta ttaacctcca ttacatatgg aattccgtcc ttcaccatca
     7141 tatcaaccct tgcataaaca ctacatttta atgctttata acaagccaat gacgctttat
     7201 ttacacgttc cttaatttca gttggaagtt caataacttc ttcaattgta ctaacatcat
     7261 cgtattttgc attgtagtca aaaaactcag ctgcatgtcg aattgaaacg ataggtaaat
     7321 gtttaccagc aaaaattgaa caagtaatct cgtcaccttt tatatacttc tcaattacta
     7381 cttcagaatc ccattcgaat acagtttcca gcattgagat caattcattt ttatcattga
     7441 cgatctttac gccaacactc gagccgccag agtttggttt taccactaac ggaaatccta
     7501 acttatctaa atcatcaagc tttagatccg ccatttttgt aagttcaatc caatctggtg
     7561 tttctactcc ttcataacgt aaaatctttt tcgaaatatt tttatccata catataccgc
     7621 tagataacat attgctgcca ctatatggaa tacctaaact ctcaagcgtc ccttgaaccg
     7681 taccatcttc tccgtatttt ccgtgtaatg ctagcaatgc aaaatcaatg tctttcgctt
     7741 tttcaattaa atccattttt tcatttaacg taattggaac gatttcatac ttatttttat
     7801 ctaaatgtgc aatcatttca tttccagtca taattgatac ttgtttttca gaggatactc
     7861 ctcccataat aacgccaatt ctcattttct caactccaag tttcattttt taaagtttca
     7921 ccaattattt gaatcccact aactatttct ttctccttca aacgtgaaaa acctaagcgg
     7981 aatgtattcg ctccccctcc atcagaataa aaaacatctc caggagaaaa cgttacacct
     8041 ttctcataac acttttgtaa aagtgtgcga gcgtgaattt tctcttccaa ctctataaac
     8101 aaatgaagtc cgccatctcc agtcatcctt ttaaacggaa tgtactgatt gcaagcccca
     8161 acggctaact catatttttt cttgtaaaca gatcttgcct tttttaaata tttttcaaaa
     8221 tatccttcat gtaaatattg aaacaaaaca gcttgatcta acgtagatgt gtgaatggtt
     8281 cttgctcttt taacactttc taaataatga atcagttctt tatcggcaat aatccaccct
     8341 acacgtaaac cagggaaaag taccttcgaa aagctactaa tataaatcac attattacca
     8401 gcccctgcaa aagttaatag tggcgataaa tgtgaacctg aataacgtaa ttcttcatta
     8461 aatccgtcct ctataatagg aaccttatat ttcgaaaata acctcatcaa ttccgttctt
     8521 ttctccgaac ttgtaacaat accagttgga ttatgataag aaggaattaa atacgcaaaa
     8581 tcaaaatcct tttcacgtaa acttttttct acttcagtcg tatcaatacc atcatcattc
     8641 atatctatcc catgaacttc aagtccatgt aagcgaaaaa gtttcaatgc agcatggtga
     8701 gttggattct cacaaatcac acgccctgat tttttcgcta aggaagatag tacaatatct
     8761 aatccttctg taaatccatt tgtaattaaa atatctttgt tcgaaatatc tacacctttt
     8821 atttccatgt aatgaagtag atatttcatt aacgggcggt aaccttttgc atatccgtag
     8881 ttcaatacga tatccccttc aatggacata cgagtaagaa aagctctttt gaaattttca
     8941 acatcaaata gcttttcgtc cggggctata ctattaaaaa caaccattcc tttttcccag
     9001 cggactccat gcttcattaa gtctaattca tccgctaatg tggtaactgt attaagttta
     9061 tttttccaat ctatttcaac agacgaagtg ttagatatat ccaccttcgc aacgaaattc
     9121 ccttttcctt ttactgcata aatgagtcct tcttgttcta aatccgcata agcagagagt
     9181 actgtatttc tgctcactga taataattca ctcagttccc ttgttgatgg gatcttttga
     9241 tcaccgagca aatgcccttt cataatcatc tttttaaaat aatcctttaa ttgaatataa
     9301 acgggacgat cttttacaac tttaaaatct ttaaacaatt tcttaccccc tgcaattatt
     9361 tttgcatatt tatttcattc tcaaaaggtc cactacatat gtatttttta ttaacagcgg
     9421 tacggtctta taaagtgaaa ctttaatccg tgggaatttt attcatcccc caccgatttt
     9481 tagcccgaat cggacatata tgggctaaaa ggctcccacc taactttttt tctccatccg
     9541 aattttgaag gtgtgagttt tacttctata aatctctacg atataagtta attacatctt
     9601 ttaaagcagc tgctgcggct ccgcgtggat tagaagctcc accaattgta gtaacttgtc
     9661 ccattgtatc tgtaaagaat gttattccct tttcagttcc atttactttt gctagtggat
     9721 ggtctttttg tatttcaagc ggttctacat taagattcac tttaccatcc atatccttat
     9781 atgctgaagc tattaattta atataattgt tttgttcctt agcatgtcga atttgttgtt
     9841 ttgtaacatt ttcgattcct tttatatgca tatcagtaag catattctca acacccatca
     9901 agctgtttgc caaaagtaat aatttacacg cactatccga accacttaca tctaatattg
     9961 ggttcgtctc agcaattcct ttattttgcg cttctttcag tgcttcttca aacgtaacat
    10021 cttcttcata catttttgta agaatataat tagttgtccc gtttaatatt ccttctattt
    10081 tttcaatatt gcatccagcc aaactaaatt gtccaatatc taatgtcggt agtgctgcag
    10141 cagttgcgcc actatagcga attcgtacat tcgcttcttt tgctgctgcc tttatttctc
    10201 tccagttagt aacgagtgca ccttttgaaa ttgcaacaat atccatatgt ttctcaatcg
    10261 cttgttttat atattttttt cccggatttc catctttaag atttgtaact gttgattcta
    10321 ccaa
//
