LOCUS       NZ_NVOB01000020        10267 bp    DNA     linear   CON 01-APR-2024
DEFINITION  Bacillus toyonensis strain AFS095804
            AFS095804_119_G5_Contig20_140618, whole genome shotgun sequence.
ACCESSION   NZ_NVOB01000020
VERSION     NZ_NVOB01000020.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus toyonensis
  ORGANISM  Bacillus toyonensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 270976)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Large-scale bioinformatics analysis of Bacillus genomes uncovers
            conserved roles of natural products in bacterial physiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 270976)
  AUTHORS   Bleich,R.M., Grubbs,K.J., Santa Maria,K.C., Allen,S.E., Farag,S.,
            Shank,E.A. and Bowers,A.
  CONSRTM   Agbiome Team Llc
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Chemical Biology and Medicinal Chemistry,
            UNC Chapel Hill, 125 Mason Farm Rd., Chapel Hill, NC 17599, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Assembly Cell v. 4.2.83
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 54.85x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002552225.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 01:56:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,087
            CDSs (total)                      :: 6,001
            Genes (coding)                    :: 5,774
            CDSs (with protein)               :: 5,774
            Genes (RNA)                       :: 86
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 79
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 103 of 227
            Pseudo Genes (incomplete)         :: 136 of 227
            Pseudo Genes (internal stop)      :: 60 of 227
            Pseudo Genes (multiple problems)  :: 61 of 227
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:33:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 205895
            Orig. end                         :: 216162
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NVOB01000020.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10267
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10267
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10267
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5267](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5267)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    892..905
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 25.47"
                     /tool="antismash"
     misc_feature    complement(1026..1041)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.2"
                     /tool="antismash"
     gene            complement(1088..1531)
                     /locus_tag="CON62_RS17355"
                     /old_locus_tag="CON62_17360"
     CDS             complement(1088..1531)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757631.1"
                     /locus_tag="CON62_RS17355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_17360"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_000757645.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDKTNEKILMVKNKGKNGSYYTLPGGSVKFGETL
                     EEAVIREVKEETGLHITVKGICSISEAFFEERGHHAIFFNFLGEIIGGETYISRPKEIE
                     EITWMELHIAAPYLRIPEQLLDFLQRKETVPYFFNGTIVHQSL"
     gene            complement(1672..2088)
                     /locus_tag="CON62_RS17360"
                     /old_locus_tag="CON62_17365"
     CDS             complement(1672..2088)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016104977.1"
                     /locus_tag="CON62_RS17360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_17365"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_000706728.1"
                     /transl_table=11
                     /translation="MKIVKQWVQEDSDYIREKVIEYNQKHLSNEEKTPSEKVSFIVKNE
                     KEEIVGGITAITFWHHVHVDFLWVSEECRHEGYGSKLIKLIEEFAIEKGCRLINLDTFS
                     FQAPDFYKKHGFIVIGVSEDHPKGHNHYYLEKRL"
     gene            complement(2222..3430)
                     /locus_tag="CON62_RS17365"
                     /old_locus_tag="CON62_17370"
     CDS             complement(2222..3430)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="CON62_RS17365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_17370"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_000815141.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAAKLQRKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLIDSLGVS
                     QAFLYWGIIVLLLVLIGSLFLREAIVSNTVTETLHNDYTPREMMRTKQVYLLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAVFNTVGRIILGTLSDKIGRMKIVSA
                     TFIIIGVSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGALLGGFQPTFITIGVLCAISFIISILIRPPNVEKKKEIKQLHR
                     KIA"
     gene            complement(3711..3854)
                     /locus_tag="CON62_RS32095"
                     /old_locus_tag="CON62_17375"
     CDS             complement(3711..3854)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="CON62_RS32095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_17375"
                     /product="hypothetical protein"
                     /protein_id="WP_000653682.1"
                     /transl_table=11
                     /translation="MKDKVSKDKKQNIKDVNTEQNAIFSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            complement(3886..4902)
                     /locus_tag="CON62_RS17375"
                     /old_locus_tag="CON62_17380"
     CDS             complement(3886..4902)
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="CON62_RS17375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_17380"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_000288924.1"
                     /transl_table=11
                     /translation="MYTYEKLISWVENIKEKNHSSATALCIIKDNKIVLEHYSGYHSNT
                     STSKKVTASSQFNVASARKSYLGFMVAYALYDGKINSIDDEAIKYFKDFDPALLDKTTI
                     RHLVTHSHGLGETDDGTIFREFEAGQDWAYRDINVRMMTHLIYRLYNKSFPELLKERVF
                     TPANFQETGWRIQQNENLVNVVNNPNEDAISEVGTVDDGTEKNLFVSAREFAYWGNLHL
                     NQGMINGKQVVPKEVIKIATSLQSPAYKNNELPQNGLFWFVQNEPKQLSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFTSCNRA"
     gene            complement(5001..5267)
                     /locus_tag="CON62_RS17380"
                     /old_locus_tag="CON62_17385"
     CDS             complement(5001..5267)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071381.1"
                     /locus_tag="CON62_RS17380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_17385"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_001071376.1"
                     /sec_met_domain="TIGR03601 (E-value: 6e-49, bitscore:
                     158.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQSLNLNDYQSGNVVYWDQQQNQYPYYYIQDDARRCGG
                     CGGCGGRCGGCGGRCGGCAGRCGGCVGCAGCFSCFNCWNWWII"
     gene            complement(5465..5854)
                     /locus_tag="CON62_RS17385"
                     /old_locus_tag="CON62_17390"
                     /pseudo=""
     CDS             complement(5465..5854)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="CON62_RS17385"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_17390"
                     /product="NUDIX domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MIAEYIGHHYFLPGGHVEIGESAENALIRELREGA"
     gene            complement(5935..6393)
                     /locus_tag="CON62_RS17390"
                     /old_locus_tag="CON62_17395"
     CDS             complement(5935..6393)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="CON62_RS17390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_17395"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_001102693.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRENWEEALQLQVKEDQRKFVPAVAVSLAKVYIKPDG
                     DNVEYIPFAIYVSNLMVGFIMHAVVRETSNMYWINGFIIDQKQQGNGYGKSALQESIYL
                     IKNKFKACKEIRLTVHKENISAKKLYEHYGFQPLGQDYDGEQVYRLFV"
     gene            complement(6470..7393)
                     /locus_tag="CON62_RS17395"
                     /old_locus_tag="CON62_17400"
     CDS             complement(6470..7393)
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="CON62_RS17395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_17400"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_000416768.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATKPLTEEISTYYPEIHGLEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGKDLTIVDVGRSTSLAIAFNLWNDLILNVKGIYFMGGVFLEVGNVTPLA
                     EANVYGDPIASKIVFQQAKNLFIFPLNVTNKAVLTPNVFNYIQANSKNPFHTIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVVAISGLVNPSFFEFAHRTVNVDIYGDTKGQTFADFRPS
                     SISKGARIALEIDEQKFIQDFIKIML"
     gene            complement(7557..8918)
                     /locus_tag="CON62_RS17400"
                     /old_locus_tag="CON62_17405"
     CDS             complement(7557..8918)
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080598.1"
                     /locus_tag="CON62_RS17400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_17405"
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_001080595.1"
                     /transl_table=11
                     /translation="MNRKRNIFICLSIIGIGLASVSNSSFAANVTDSSVQHSIPVMNQQ
                     VAVATEMKPFPQQVNYAGVIKPNHVTQESLNASVISYYDNWKKKYLKNDLSSLPGGYYV
                     KGEITGDADGFKPLGTSEGQGYGMVITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNL
                     MGWVVADNKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGSVNYLKEAQNMITKGIKAS
                     NVTNNNRLNLGDWDSKSSLDTRPSDWMMSHLRAFYEFTGDKAWLTVINNLYDVYTQFNN
                     KYSPNTGLISDFVVKNPPQPAPKDFLDESKYTNSYYYNASRVPLRIVMDYAMYGEKRSK
                     VISDKVSSWIQNKTNGNPSKIVDGYQLNGSNIGSYSTAVFVSPFIAASITSNNNQKWVN
                     SGWDWMKNKRESYFSDSYNLLTMLFITGNWWKPVPDDKETQNLINDEMYEGYDK"
     gene            complement(9057..9971)
                     /locus_tag="CON62_RS17405"
                     /old_locus_tag="CON62_17410"
     CDS             complement(9057..9971)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017673335.1"
                     /locus_tag="CON62_RS17405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CON62_17410"
                     /product="DMT family transporter"
                     /protein_id="WP_098009531.1"
                     /transl_table=11
                     /translation="MLNKKWDLQIICAHAFTILIWGTAFPTIRMGLESYTPEHLTLLRL
                     LIASFILLVFSFIYKLRLPDLKDIPAIFIFGALGFTIYHIALNYGEKTVNAESASLIIS
                     VTPIVTAILASVFLNEKMKLNGWIGGVISFIGIALISFSQGGSIQFNSGGLFILLAAVS
                     ESLFFVFQTSYLKKYGFLPFTIYTILSSTVCMLIFLPGIYTETLAAPLEVNVSVIYLGL
                     FPTVLPYLALAYIISRAGASEATSSLYLTPVTACFIAWVWLGEVPTFISVIGGGITILG
                     ILIAHIRVVRKEKHRNLVNNKSI"
ORIGIN
        1 ttgaaccacc aaaacttaaa gtaaccattt cttctgttat tgcccttaaa gcatctacca
       61 taaatcgtcc gtcgaaagat atatttaatt gtttttcacc ttgaatcgca tctacttgtt
      121 gtgtttcagc tattttacca atctgagaag cgttagaaga aattttaatt gtggattcat
      181 tgatgatttc taagttgacg ttgttatttg cccattcact tgctaataag ctcgatcgat
      241 ctacaccttg taaaatcttt tttctttcta cgttaataac cgtttgaaat tcatttggaa
      301 tgagattaga tatattagga tatttccctt caatgagtct tgaatataac gtagttgttc
      361 cgaatgtaaa aataacgtga ctgtctgaaa ggtatatgta tacgaattcc gtattactgt
      421 tcattaattt tagaagttca ctaatagttg cgcttggtac gatacaattt gctttcgcat
      481 ctgaagaaat tggcgtttca cgtatagcta gtctatgtga gtcagtagca gcgcaaacta
      541 atttattgtg atctaactct atatgtactc cggtgagaac aggtctggat tcatttttag
      601 ctactgcaaa aacagtttgt ttaaacactt caatcaattg ttctgtttct acttgtattt
      661 ctgcatgatc ccctataagt ggtacattcg gaaactcatt tgctggaagt ccatttaagt
      721 ttaatgtgat ttcttctgat tgtatcgtaa tgatttgctc atttttactt tttattacta
      781 tatcacttgg cattttcttt ataatttcaa tgaaatattt tgcaggtaca acaatagttc
      841 ctgcttgtaa tatagttgca atctgttcgt cttcgattga attaggtata aattttttaa
      901 taaaaacatt cgagttactt gcgattaaag taattccaga ttgatcggct gttattttga
      961 taccagataa tataggaatt aaaacttttg tagatatagc tttacttact tccgaaagtg
     1021 cttgagtgaa atgtttgtga ctaacgataa actccatttt ttccccgcct taaatgaaat
     1081 aatttattta taaagattga tgaacgattg ttccgttaaa aaagtaaggt actgtttctt
     1141 tcctttgtaa aaaatctagt aactgttcag gtatacgtaa atacggtgct gctatatgta
     1201 attccatcca agtaatctct tcgatttctt ttggacggga tatatatgtc tctccgccga
     1261 ttatttcacc taaaaaatta aagaaaatgg catgatggcc tctctcctcg aaaaaggctt
     1321 cgcttatgga acaaatcccc tttacagtta tatgtagccc tgtttcttct tttacttctc
     1381 gaatgactgc ctcttctaac gtttccccaa atttaactga gccacctggt aatgtgtaat
     1441 aagacccatt ttttcctttg ttttttacca ttaagatttt ttcgttcgtt ttatcgtaca
     1501 aaagcgcgta tgtaacatta acctttttca tatttacccc tttattaata agaattttct
     1561 gcctctattt cattttacca gatacataca aattaaaaag aacgtatttn naaatacgtt
     1621 ctttttaatt tattgaattt tatatattga gattaattaa ttctatacac tttataacct
     1681 tttctctaaa taataatgat tatgtccttt tggatgatct tcactcactc caatgactat
     1741 aaatccatgt tttttataaa aatccggagc ttgaaagcta aacgtatcta agttgattag
     1801 cctacatcct ttttcaattg caaattcttc gataagtttt attaatttac ttccgtatcc
     1861 ttcgtgtctg cattcttcag aaacccatag gaaatcaaca tgtacatggt gccaaaatgt
     1921 tatagctgtt atcccaccta ctatttcctc tttttcattt ttcactataa aactaacctt
     1981 ttccgaaggt gttttctctt catttgaaag atgtttttga ttatattcaa ttactttctc
     2041 tcgtatgtaa tcactatctt cttgaaccca ttgctttact atcttcattt tacatatcac
     2101 actcctttta ttttatctat tccatttcat aattgtattg tcctgtcttt actaatcaat
     2161 gaaaggagca tagttaacgt tgtaatgaaa aagagcccta cgataaggcc tctttttaaa
     2221 actaagctat ttttcgatgt aactgcttga tttccttttt cttctcgaca tttggcggac
     2281 gaattaatat agaaataatg aaagaaatcg cacataaaac acctattgta ataaaggttg
     2341 gttgaaatcc accaagtaat gctccaatga atgaacctgc aagtgcacca aatccgaaac
     2401 cttgatatac aatcccgtaa tttgtactat gattttttaa accgaagaaa tctccgacaa
     2461 tggctgggaa tattgtaata ttaccgccga agcaaaaggc gacacttgct acacaagcaa
     2521 aataaatccc gtaatttaat ggaatgaaac taagagtaaa gacggatact ccaataataa
     2581 taaatgttgc agagacaatt ttcattctac caattttatc tgataacgtt ccaagaatga
     2641 ttctacctac tgtattaaag actgcaatca ttgcgacggc gttagcggcg gttgctgcgc
     2701 taagtccaac gagttgtacg ccgatatctt ttaccatacc gattaagtac agaccaccca
     2761 tacacgatgt gaataacatg aaaaacaata gatatacttg tttcgttctc atcatttcac
     2821 gtggcgtata atcattgtgt aatgtttctg ttaccgtatt acttacaatc gcttcacgta
     2881 agaagagcga tccgattaat actaaaagta agacgataat gccccaatat aaaaatgctt
     2941 gtgatacacc taggctatcg ataagatttc cgtttatata tctaaaaatt aaactgccca
     3001 ttccatatgc tgaaacagat attccagaaa taagtccttt tcgatttgga aaccatttaa
     3061 ttaaattaga tagtgacgta atataagctg ttccatccgc atacccaaca acaacaccag
     3121 ctaataaata tagtaatggt aaggaagaaa cttgtgaact aagtattaaa ccgagtccta
     3181 gaacaattcc tgcagtagcg ataagtttac gaagtcctaa ttttcgctgc aactttgctg
     3241 caaacaaggt tgaaaatgat aaagaaaagc ttgttatgga gaaagttatc gcaactgaat
     3301 tgagattcca tccgaactta cttacaaggg gttgattaaa taaactccat gtataaattg
     3361 ttccaaggcc aatttgaaca atgattgtac ctagaacaat taatagcgga tttatagaag
     3421 tttgtttcat actaactgtc cccttctttt atcttgacat gaattttcac agttagtata
     3481 tccatcataa attgcaagat actgtacgaa aaatggaaat taagtttatg tacacaaaaa
     3541 gacacttcat catgaagtgt cttttcgcag gttatatcag atacatttaa agaaagcttt
     3601 gttaagataa gccattttca agtgcttatg tatatatgtt atcaaatatt ttcttacatg
     3661 tgatgggact taagacgtat tttttattcg tcatatagtc ggatgagaaa ttaatattcg
     3721 atttcatcaa aatctttcgg ctgcggtaca gtttgcatgt tagctgcatc tttcggatcg
     3781 ctaaaaatcg cattttgctc tgtatttaca tcttttatat tttgcttttt atctttgctc
     3841 actttatctt tcaaattctt tcactccttt ttgttatagg agtaattatg ccctattaca
     3901 gctagtaaat gtatcggcga ctaaattgct aaattcacgt aaataatata agtaattatc
     3961 accgccgtag ttatatcttt tattgtacat tttcgcaaca actacattat actcaggtat
     4021 tactaaaatg gttggtccag taattcctaa tatttgatac gaaccttttg gaacacgttc
     4081 accaagttca cttaattgtt ttggttcatt ttgaacgaac caaaacaacc cgttttgtgg
     4141 tagttcatta tttttataag ctggactctg caagctagta gcaattttta taacttcttt
     4201 tggaacaact tgtttaccat ttatcatgcc ttgatttaaa tgaaggttgc cccaataagc
     4261 aaactctcta gctgagacaa acagattttt ttcggtaccg tcatctactg taccgacctc
     4321 actgatcgcg tcttcatttg gattattaac aacgttaact aaattttcat tttgctgtat
     4381 tctccatcct gtttcttgga aattagcagg agtaaataca cgctccttta acaattcagg
     4441 aaaactttta ttataaagcc gataaatgag atgcgtcatc attcttacat taatatctct
     4501 atatgcccaa tcttgtccag cttcaaattc acggaaaatt gtcccgtcat ccgtttctcc
     4561 tagaccgtgt gaatgagtta ctaaatgcct aatcgttgtt ttatcaagta atgcaggatc
     4621 gaagtcttta aaatacttta tcgcttcatc atcaatagag tttattttcc catcataaag
     4681 cgcatacgct accatgaatc ctaaataact ttttctcgca gaagcgacgt tgaactgtga
     4741 agatgctgtt acttttttac tagtagatgt atttgaatga tatccactat aatgctctag
     4801 tacaattttg ttatccttta taatacatag tgctgtagca gaactatgat ttttttcttt
     4861 tatattctct acccaagaaa tcaacttttc gtatgtatac aaataaatcc cccttaatac
     4921 tcataaaaaa tcacaacatg aaagaaaagt attactataa acacctttct ttcatgtgaa
     4981 atattattga ttttctatac ttaaatgatc caccagttcc agcaattaaa gcaactgaaa
     5041 catcctgcgc aacctacaca tccgccgcaa cgtcctgcac aaccaccgca tctgccgcca
     5101 catccaccac agcgtccacc acaacctccg catccaccgc aacgacgtgc atcgtcttga
     5161 atataatagt atggatattg attttgttgt tgatcccagt aaactacatt accagattga
     5221 taatcattaa ggtttaatga ttgtagttct tgttgaaact gattcattac gaacccctcc
     5281 atttataagt aattccgccc aaatgtaaaa agtttatata tttttgtaaa ccaaatagaa
     5341 acaaagctac agactaaata cgtacatcaa caaaatatga acttacacta ggtgatgtac
     5401 cttgtttttt agcctatttt ctttatatcc tttcgaagga cgtatttact atacatagag
     5461 tgatttaaaa attacttatc caacaattta gtagttcatc atttaatttt ctttctagta
     5521 attctttaac ggagggcatt ggcatgattt tataagtttt taaagattct ttactataat
     5581 ctatccaatg aaatgctaaa tgagattctc tagaatttgg tggtaaatca atttgtaact
     5641 cttttgaaga tacttcaaaa tgtggttgat ttcatgatga agtatttctt tatcttgcca
     5701 ttggttttct ataactccta aaaatcgttt tatactgcag tttacttcaa gctccttctc
     5761 ttagttctct tattaatgca ttctctgctg attccccaat ttcaacatgg ccgccaggta
     5821 aaaaatagtg atgcccaata tattcagcaa tcaataattt ttcatctttc tagtcattca
     5881 ctattacctt gattaaagta gttattttca tttcacgcta atacttaata aaagttaaac
     5941 aaataaacga tatacttgct caccgtcata atcttgccct aatggttgaa aaccgtaatg
     6001 ttcatatagt ttctttgcag agatattctc tttatgtact gttaatctaa tttctttaca
     6061 cgccttaaat ttatttttta ttaaataaat gctttcttgt aatgctgatt ttccataacc
     6121 attgccttgc tgcttttgat caataataaa cccgtttatc caatacatat ttgatgtctc
     6181 tcttacaacc gcatgcataa taaaaccaac cataagatta ctaacgtata tagcaaatgg
     6241 tatgtactct acattgtcac catctggctt tatatatact ttagcaagtg aaactgctac
     6301 ggcggggaca aatttccttt gatcttcttt aacttgtaac tgtaatgcct cttcccaatt
     6361 ctctctcgta acaaccttta actggacatt caatattgta agctccttta taaagaggat
     6421 atatttttat attcaacgta gtgaaataat tccctttttc gacaacagct tataacatga
     6481 tttttataaa atcttgaata aatttttgtt catctatttc taatgctata cgggcccctt
     6541 tagatattga gctaggacga aaatcagcaa atgtttgtcc tttcgtatct ccgtatatat
     6601 ctacattcac tgtacgatgc gcaaattcaa aaaaacttgg gttaactaat ccacttatag
     6661 caaccacatc atgtagcaaa ggcccttcga tagatggatt aagtttttgg tatgcagata
     6721 aatagtaatc atacatcggt ttcattatcg tatgaaatgg attctttgaa ttagcttgga
     6781 tataattaaa tacatttgga gttaacacag ctttatttgt tacatttaaa gggaatatga
     6841 ataaattctt tgcttgctga aaaacaattt tacttgctat aggatctcca tacacatttg
     6901 cctctgctaa tggagtaacg tttcctactt ctaaaaagac accacccatg aaatatattc
     6961 cttttacatt tagtattaag tcattccata aattaaaagc aatcgctaat gaagtagatc
     7021 ttcctacatc aacaattgtt aaatcttttc catactttac aagaattgca gcaatactac
     7081 caaaattata tatcggaata gataatacgc tttctggaac atgtatgggc cctaatcctt
     7141 ctaatccatg aatttcagga tagtacgttg aaatttcttc agtaagtggt tttgttgcgc
     7201 cgctaatgac aggaatgtct tgcctatttg ctaactgtaa aatatacgcc gcattatgtg
     7261 cagcatgtat atgctcaaca tttccatatc cagttacaat accgacaatt tcaatttcgg
     7321 gatgcagtaa accatacata atggcaaatg agtcatcaat tcctggatct ccaaaaaata
     7381 atactttttc caagaaatac tctccttcca taaaaaaata aagcaaacaa tagttatgta
     7441 tatgaaaaat gcaagtatat ctatgcaaat tttagaatgg ataatgtgtt acgggatatt
     7501 aaaaaggaac tagcgcatgc taattccttc tcccagctaa ccattatcaa attctattat
     7561 ttatcatatc cttcatacat ttcatcattt attagatttt gtgtttcttt atcatcaggt
     7621 acaggcttcc accaatttcc cgtaataaat agcatcgtta ataaattata actatcacta
     7681 aaatagcttt ctcgcttatt cttcatccaa tcccaaccac tatttaccca cttttgatta
     7741 ttattactcg ttatacttgc agcaataaat ggtgaaacga atacagcagt tgaataacta
     7801 ccaatatttg atccgtttaa ttgatagcca tccacaattt tagaaggatt tccattcgtc
     7861 ttattttgaa tccatgaaga gactttatca gagatgactt tacttctttt ctcgccgtac
     7921 atcgcataat ccattacaat tcttaatggt acccgactag cattgtaata atatgaattt
     7981 gtatattttg actcatctaa gaaatcttta ggtgcgggtt gtggtggatt ttttacaaca
     8041 aaatctgaaa taagccctgt atttggagag tacttattat taaattgcgt atatacatcg
     8101 tacaaattat taataacagt aagccaagct ttatcacctg taaattcata aaatgctcta
     8161 aggtgtgaca tcatccaatc agatggcctc gtatcaagtg aacttttaga atcccaatct
     8221 cctaaattta gtcggttatt attcgtaaca ttactagctt taataccttt tgtaatcatg
     8281 ttttgtgctt ctttcaaata attaactgat ccatttgatc cccactgctt atgagcaagg
     8341 agaagtgaat atgcaatatc taaatctcca tcagtagcag aatcaaaatg tccttgtgct
     8401 tttttattat ctgctacaac ccaccccatt aaattaggat tttgagagct tttaaaagtt
     8461 cttgctgttt taaataaacc gtcatagatt ttttgggcat tcgaatcata accagccatt
     8521 aatactgtaa ttaccatccc atacccttgt ccctctgaag ttccaagtgg cttaaaccca
     8581 tcggcatcac ccgtaatctc tccttttaca taataaccac caggtaaaga agataaatca
     8641 tttttcaaat atttcttttt ccaattatcg tagtaactta ttacagaagc atttaagctt
     8701 tcctgtgtaa catgattcgg ttttataaca cctgcataat taacttgctg aggaaatggt
     8761 ttcatttctg ttgcaacagc tacttgttga ttcatgacag ggatagaatg ttgtactgaa
     8821 ctatccgtta catttgctgc aaaactagaa ttagaaacac tagctaatcc gatacctata
     8881 atagaaaggc atataaaaat atttcttttt ctattcatta tgttgtcatc tccttttaaa
     8941 atgtttacta cataaaatgt aacatacaat atttaactga aatacaaaat attatattta
     9001 tcaacataag aaagaaacta tatcaaaaag tatcataaaa tcattatcta attaatttat
     9061 atcgatttat tgtttactag attcctatgt ttttcttttc ttactactcg tatatgagca
     9121 atcaatatcc caagtatagt aatcccacct ccaattacag aaataaaagt cggtacttca
     9181 cctaaccata cccaagctat aaagcacgca gtgactggcg ttaaatatag agaacttgtt
     9241 gcctcggaag caccagcacg agatataata tacgctaagg caagatacgg gagtactgtt
     9301 ggaaataagc ctaaatatat aacactcaca ttcacttcaa gaggagctgc tagtgtttct
     9361 gtataaattc ccggtaagaa aataagcata catacagtgc tagataaaat tgtatatatg
     9421 gtaaatggca aaaagccgta ctttttcaag taagaagttt ggaaaacgaa aaataggctt
     9481 tctgaaacgg ctgctaataa tataaataat cctccactat taaattgaat agaacctcct
     9541 tgactaaatg atatgagagc aattccaata aaactaatta caccaccgat ccagccattt
     9601 aatttcattt tttcattcaa aaaaacagaa gcaagaattg ccgtcactat tggagtgact
     9661 gaaattatta aacttgcaga ttcagcattt actgtttttt cgccgtagtt taatgcaatg
     9721 tgataaatag taaaccctaa agcaccgaat ataaaaatcg ctggaatgtc tttcaaatca
     9781 ggtaatcgca gtttgtatat aaaggaaaac acaagaagta tgaatgaagc gattaacaag
     9841 cgtagtaaag taagatgttc aggtgtgtat gattcaagcc ccatacgaat tgtcggaaat
     9901 gcagttcccc atattagaat cgtaaaagcg tgagcacata tgatttgtaa atcccatttt
     9961 ttattaagca tgttttctct tcctctccaa taaaccaacc attttcaaat tgccaattat
    10021 ttcttttcat catatttaat agttgataca atgtcttcaa ccagttattt atcgaaaaaa
    10081 ccaaccactt ttcattttta aataaacgag ggagttttta tatgacaatc gataaaaagg
    10141 tgcccaaata tcggcagata atagactata tgaaagaaaa aattgaaaac ggagaatggc
    10201 caatcggaag taagattcct agccagcggc agttagcaaa gttatttcat gtaaatcgga
    10261 gtactgt
//
