LOCUS       NZ_CP022203            11692 bp    DNA     circular CON 28-MAR-2024
DEFINITION  Corallococcus macrosporus DSM 14697 chromosome, complete genome.
ACCESSION   NZ_CP022203
VERSION     NZ_CP022203.1
KEYWORDS    RefSeq.
SOURCE      Corallococcus macrosporus DSM 14697
  ORGANISM  Corallococcus macrosporus DSM 14697
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Myxococcaceae; Corallococcus.
REFERENCE   1  (bases 1 to 8973512)
  AUTHORS   Rupp,O., Goesmann,A. and Sogaard-Andersen,L.
  TITLE     Sequencing and comparative analysis of myxobacterial genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8973512)
  AUTHORS   Bruckskotten,M., Treuner-Lange,A., Rupp,O., Goesmann,A. and
            Sogaard-Andersen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2017) Department of Ecophysiology, Max Planck
            Institute for Terrestrial Microbiology, Karl von Frisch Str. 10,
            Marburg, Hessen 35043, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2016
            Assembly Method                   :: Celera Assembler v. 8.1
            Expected Final Version            :: yes
            Genome Coverage                   :: 83.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002305895.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 03:36:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,344
            CDSs (total)                      :: 7,262
            Genes (coding)                    :: 7,199
            CDSs (with protein)               :: 7,199
            Genes (RNA)                       :: 82
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 26 of 63
            Pseudo Genes (incomplete)         :: 45 of 63
            Pseudo Genes (internal stop)      :: 2 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:23:17
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5088228
            Orig. end                         :: 5099920
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022203.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11692
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="17"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11692
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11692
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6692](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6692)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    63..74
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            78..2282
                     /locus_tag="MYMAC_RS20565"
                     /old_locus_tag="MYMAC_004071"
     CDS             78..2282
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480692.1"
                     /locus_tag="MYMAC_RS20565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MYMAC_004071"
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="WP_170114751.1"
                     /transl_table=11
                     /translation="MTRIRIGQRWKREPAASPLDSIALELDGVDLLSGAVEESLAEVVP
                     SLVRSVAALAGGRQKLAQVSLPEAHLELVLRRVGPEVELLVANLSRPARLMRPPVRVEL
                     EELVEAVREAGERFLADVTRAGPKALATPLGQALKEPLKSLKRPARPPDEVPARPATRR
                     LEPAEVPGFGFELRDAGLPTGAGRGAARGTAGLLAPLLAGGEVWLSLPGRPDAWRVPGP
                     PFLTVLELSRLAVELARAVELGERRFELAPAGAKPPLLLDLKTGQARAGRTGAPFPLPG
                     SVLAAALFHLGESLAAAFAEADRALVGNPYRAELAERCREGLSHLRGPVQPPEAQGAAR
                     EKKARAKGQGTSRPLKVPGRLRRLRFEKLWEKRGLPDAEEARLLLGRHGPVYCAPQLTS
                     AFSRKDGALLWKRAAALGVAASADGHAVAADIARVYGFTGRGSGARWLHDHDGIPLGPL
                     LLRKDGLLLTLSEDRTVVAFAEATGREVWRLAPPRTQRSWLATQGHRALVGTDSGYLYG
                     LDLEDGQVRYRMRAPMPFHGAPVAWGKRFLAMLGRGSHWALLLADAHTGEAVWTYEPDL
                     SHLSAPLPVGSRVFIAGEREREGVLLCLDAKGRRLWERPLNLGPGPYALAPLPRAVVVT
                     SASGAATRVAASGTVDWRVGAAGEPLIGALPAHTARDVTLVPGERVRAVDPRGGQVLAE
                     VQAGVGLVALQADVRLNLYFLDDAGTLSAYRLGSHFTVVE"
     gene            complement(2296..3213)
                     /locus_tag="MYMAC_RS20570"
                     /old_locus_tag="MYMAC_004072"
     CDS             complement(2296..3213)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940767.1"
                     /locus_tag="MYMAC_RS20570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MYMAC_004072"
                     /product="hypothetical protein"
                     /protein_id="WP_204816841.1"
                     /transl_table=11
                     /translation="MKRYFGVIVIVAGVLLGAVMSYRSASGRALEAQREADQQRIHAEY
                     LERVGWMRSNPDEASYRDELKPFFKNYFEQVDDHLTRFQGNTKFDDYLLELEQREERGA
                     KDDRANDRKAFYEYARKTFDSMREGRYRPVWTATDKGMRLDLISSDVVMVLGKPQIRLQ
                     LAVWGAQRTLKDDGKVLKMVTSAAFDTVWKLTDAKGKLLGEMRGADPSMKIDHPERFIK
                     AFPPQMVLGHYDLDLLPNEVSKMEMTINLTSSAASGGTATSTYVWKVDEVPSDWKLGAG
                     ESWEGATQEERPEEEIDPSKAARD"
     misc_feature    complement(3243..3257)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.07"
                     /tool="antismash"
     gene            3265..4296
                     /locus_tag="MYMAC_RS20575"
                     /old_locus_tag="MYMAC_004073"
     CDS             3265..4296
                     /NRPS_PKS="Domain: Abhydrolase_1 (81-326). E-value:
                     2.9e-10. Score: 32.1. Matches aSDomain:
                     nrpspksdomains_MYMAC_RS20575_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554161.1"
                     /locus_tag="MYMAC_RS20575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MYMAC_004073"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_095959307.1"
                     /sec_met_domain="PF00561 (E-value: 3.2e-10, bitscore: 38.8,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIQATGTHLFTTPLPLEEGELLGNPQVAWAAYGEPSDGKAVVLLH
                     DLAHSHLALGPPEDSAYQPSGWGRALVGTNLALDPAVTPVLAPGLLGSPFGSTSPATVN
                     PASGERWGLELPPLTVQDMARAVSATLRACGLSHVRALVGVGLGGHVALRLASLFPDLA
                     DGVVTLGAARALPEAVREKLGLSWQVLRADPDYRDGLYALDAPPRKTMRKLRLDFQKLL
                     AGREHLTATYRDAESARAALDAEADAFADAFDAVSWASLCSAYAGSDVSDGFARIRSRV
                     LLIAGASDAMAPVNRVRDTYHLLSAAGVSARFLELPGPGDHAALLQDSERLHGPLSDFL
                     RRC"
     aSDomain        3508..4242
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MYMAC_RS20575_Abhydrolase_1.1"
                     /evalue="2.90E-10"
                     /label="MYMAC_RS20575_Abhydrolase_1.1"
                     /locus_tag="MYMAC_RS20575"
                     /protein_end="326"
                     /protein_start="81"
                     /score="32.1"
                     /tool="antismash"
                     /translation="VTPVLAPGLLGSPFGSTSPATVNPASGERWGLELPPLTVQDMARA
                     VSATLRACGLSHVRALVGVGLGGHVALRLASLFPDLADGVVTLGAARALPEAVREKLGL
                     SWQVLRADPDYRDGLYALDAPPRKTMRKLRLDFQKLLAGREHLTATYRDAESARAALDA
                     EADAFADAFDAVSWASLCSAYAGSDVSDGFARIRSRVLLIAGASDAMAPVNRVRDTYHL
                     LSAAGVSARFLELPGPGDHAALL"
     gene            complement(4297..4932)
                     /gene="udk"
                     /locus_tag="MYMAC_RS20580"
                     /old_locus_tag="MYMAC_004074"
     CDS             complement(4297..4932)
                     /EC_number="2.7.1.48"
                     /GO_function="GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="udk"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554162.1"
                     /locus_tag="MYMAC_RS20580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MYMAC_004074"
                     /product="uridine kinase"
                     /protein_id="WP_043711474.1"
                     /transl_table=11
                     /translation="MASPLVVGIAGGTASGKTTVARKVREALADCRVAFIDQDSYYRDL
                     KDLPLADRREVNFDHPDAFDRELLVKHLKALKSGQPIQKPVYDFVTSSRQPQTKNVEPG
                     DIILIEGILVLHMKEVRDEMDVKIYVDADDDLRILRRLTRDIKDRGRDFDHVVSQYLRH
                     VRPMHMGFVEPSKHFADIIIPHGGNNEIAIGMLVGALRGKLSGVAQRD"
     gene            complement(5001..6692)
                     /locus_tag="MYMAC_RS20585"
                     /old_locus_tag="MYMAC_004075"
     CDS             complement(5001..6692)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554163.1"
                     /locus_tag="MYMAC_RS20585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MYMAC_004075"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_095959308.1"
                     /sec_met_domain="DUF692 (E-value: 1e-22, bitscore: 79.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVAAGRVYVEEMTHAAPPSWTLPWRGLGLSSNLSMADVPQPYRL
                     LDASPGLFDYVEYSAPLSLEESKAHASLFPEMWRRRQDVPVLFHPVHLNLWGPGLEPAR
                     ALVELDAHARAVESPWVGNDVGWWHVEGQPFPGYLYFTPPFNEAGLEASVAHALHVQRH
                     LSMPLVLENPAVLARRGQWHVLDFMAKLHARTGAPLLLDLGHLFSHQLSAGLPLETGLE
                     GFPLDQVIELHIAGGVVTHRGPRRFYVDDHTQPVREELFELLASLIPRCPSLRAVTFEG
                     DGHPPEVAAASLRRLRELVPAGARPPLTWRVPEASAAPVPALTEDSQPWALFDAGYGAA
                     PVTSVEDEAGTLADQDFRLAVVAEVLDRDWPLTRLLLAGTREALAAFTGSKEFRKLFSP
                     LGRSPGQAFASWAMRRLRESPDEGGSAALTLEMLLPSLFLRRVPAPAPGQVGLMEDVRA
                     GSLPVDLSELVHAARAVRRHLTARAWASGVLELSGLESLNQVARRAGPGPWSFTARRRA
                     GGLEVTTVSPAMGEFLRMLAVQPLTEAEVAPGLVEEARQRGLIRRG"
     gene            6773..7588
                     /locus_tag="MYMAC_RS20590"
                     /old_locus_tag="MYMAC_004076"
     CDS             6773..7588
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940771.1"
                     /locus_tag="MYMAC_RS20590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MYMAC_004076"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_095959309.1"
                     /transl_table=11
                     /translation="MPPASRAEIDESNAQYRGAWRLFALGSKAGEVVERPDVYITACNV
                     AWSMMNAAFLRAPVETEQALAAAAASAARYFSAGKHGWSFLICDDWLAPAVRDNAPSIL
                     DWYGLKPELVVTGMVADRLQPSLHPPSPFDLRPVTDAGGRQAVADINALSYDVPQHLGR
                     EAFDEATLYNADCQGFVACRDEEPAASTVVLRVDAAAYVALVATHPQHRRQGAAEAVMR
                     HALARARQDWGTERTVLHATEAGQPVYTRLGYRPVTRFRMYLAPPPGQG"
     gene            7767..8600
                     /locus_tag="MYMAC_RS20595"
                     /old_locus_tag="MYMAC_004077"
     CDS             7767..8600
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940772.1"
                     /locus_tag="MYMAC_RS20595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MYMAC_004077"
                     /product="hypothetical protein"
                     /protein_id="WP_204816843.1"
                     /transl_table=11
                     /translation="MAKWDDLVLAIPPHAGGRLEVPAPAALLETLLGLHNVQLTLLRGL
                     RRELRPLQEGPFHAGRTHLEAARVAHASRDEQLERLKAARGCFTEAMGMERDKARLSFI
                     ALHLGLCWLLLGAERDAREWTQLAHRTAVEAMKDILVDASKHQGLTRNRYLAPAITFFM
                     FFTSAATLGAGYLFWDRVLSKRTGTVMRRALVQMQSLADYVDALGEIRLRLGELSHDVA
                     RYELTHGVDEQQFVARITIRKLINDREAIYFNGRETRKVTWNDQERSEVITRELL"
     gene            8908..9600
                     /locus_tag="MYMAC_RS20600"
                     /old_locus_tag="MYMAC_004078"
     CDS             8908..9600
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554167.1"
                     /locus_tag="MYMAC_RS20600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MYMAC_004078"
                     /product="response regulator transcription factor"
                     /protein_id="WP_095959311.1"
                     /transl_table=11
                     /translation="MAERPTVLVVDDDPHLREIVRFALEQGGFRVDEATDGRAALAQVD
                     RALPALIVLDIMMPELDGLAVCREVRRKHELPIVFLSSRDDEVDRILGLELGGDDYLTK
                     PFSPRELVARVKAVLRRARPAPEAEAPPARMQSRGPLRMDAERWRAWWRDTEVVLTVTE
                     FQLLATLLRVPGKVFTRDELMNRVYDDVVVSDRTIDSHVRRVRQKFASAGGDVIETVHG
                     LGYRLALP"
     gene            9569..11209
                     /locus_tag="MYMAC_RS20605"
                     /old_locus_tag="MYMAC_004079"
     CDS             9569..11209
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940774.1"
                     /locus_tag="MYMAC_RS20605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MYMAC_004079"
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_095959312.1"
                     /transl_table=11
                     /translation="MALAIGSPSPERRARPRLWLVFAAVGVAGFALTLAGLAFVRVYDN
                     QLIRQTESELIAQGAVIAEVFRERLRDAEPRDDHGRERTAPWPFPLPDDTRLRPILPSL
                     RASDATLPSGGTPQPSRVPAEPLAHAAGLRLTPLLEQVRAATLAGIRVVDPEGVVVASS
                     SASLLGTTLADRTEVQRALRGEPVSVLRRRVAEPEDTPLASLSRDTGIRVSVALPVLDG
                     ERVWGAVVLTRTPMTFAKATYADRWNLTATGFVLLGVVALMSLAAAALVGRPVRALMRQ
                     TRAIALSDPSGFEPVARPVVAELAELSESLAGMATALRDRNQYIRSFAANVSHEFKTPL
                     ASIQGAVELLRDSADAMSPEQRARFLANVDADARRLTRLVQRLLELARADSMTATPAQV
                     ALGPLLEGLALRARAEGALDIHVTPPPAGLTMGLPQEVLDDVLWQLVTNARQHGGDTVR
                     VDLSVEQAPEADLVRVVVKDSGRGISEANRARIFDAFFTTARARGGTGLGLTIAQSMLR
                     AFNARLELLPSATGTTFAVVAPVRGLQRK"
ORIGIN
        1 gaacggttac aaaaatgtag gtccgtatag gcggcggatg ctagccatcg ctaaactggc
       61 ttgtcaacga acgcaagatg acccgcatac gcatcggaca gcgctggaaa cgcgaacccg
      121 cggcttcccc gctcgattcc atcgcactgg aattggacgg ggtggacctt ctgtccgggg
      181 ccgtggagga atctctagca gaagtcgtcc cctccctggt gcgctcagtt gcggcgctgg
      241 ccgggggacg gcagaaactg gcccaggtct ccctgcctga agcccacctg gagctggtgc
      301 tccggcgcgt gggcccggag gtggagctcc tggtggccaa cctctcacgc cccgcccggc
      361 tgatgcgccc ccccgtccgg gtggagctgg aagagctggt ggaggccgtc cgtgaagccg
      421 gcgagcgctt cctcgccgac gtcacccggg ccgggcccaa ggcgctggcc acccccctgg
      481 ggcaggcgct caaggagccg ttgaagagcc tgaagcggcc cgcccgcccc ccggacgaag
      541 tccccgcccg ccccgccacg aggcggctgg agcccgcgga ggtcccgggc ttcggcttcg
      601 agctccggga cgcggggctg cccaccggcg cgggccgcgg cgccgcgcga ggcacggccg
      661 gcctgctggc cccgctgctc gcgggcggcg aggtctggct gtccctccca ggcagaccgg
      721 acgcctggcg cgtcccgggc cctccgttcc tcacggtgct ggagctgtcg cggctggcgg
      781 tggagctggc ccgcgcggtg gagctgggcg agcgccgctt cgagctggcc cccgcgggcg
      841 cgaagccccc gctgctgctg gacttgaaga cgggccaggc cagggccggc cgcaccgggg
      901 cgcccttccc gctcccgggc tccgtgctcg ccgccgccct cttccacctg ggcgagtccc
      961 tggccgcggc cttcgcggag gcggaccggg ccctggtggg caacccctac cgggcggagc
     1021 tggcggagcg ctgcagggag ggcctctccc acctgcgcgg ccccgtccag ccccccgagg
     1081 cgcagggcgc ggcccgcgag aagaaggccc gcgcgaaggg tcagggcacc tcgcgtccgc
     1141 tcaaggtccc cggccgcctg cgccggctgc gcttcgagaa gctgtgggag aagcgaggcc
     1201 tgccggacgc ggaagaggcg cggctgctgc tgggccggca tgggccggtc tactgcgccc
     1261 cgcagctcac ctccgcgttc tcccggaagg acggcgcgct gctgtggaag cgcgccgcgg
     1321 cgctgggcgt ggccgcgtcg gcggacggcc acgcggtggc ggcggacatc gcgcgcgtgt
     1381 acggcttcac gggccggggc agcggcgccc gctggctgca cgaccacgac ggcatccccc
     1441 tgggcccgct gctgctgcgc aaggatggcc tgctcctcac cctgtccgag gaccgcaccg
     1501 tcgtggcctt cgcggaggcc accggccgcg aggtgtggcg cctggcgccg ccccggacgc
     1561 agcggagctg gctggccacg caggggcacc gcgcgctggt ggggacggac tcgggctacc
     1621 tctatgggct ggacctggag gacggccagg tccgctaccg catgcgggcg cccatgccct
     1681 tccacggcgc gccggtggcg tggggcaagc gcttcctggc catgctgggc cggggctcgc
     1741 actgggcgct gctgctcgcg gacgcgcaca ccggtgaagc cgtgtggacg tacgagccgg
     1801 acctgtcgca cctgtcggcg ccgctgcccg tgggctcgcg ggtgttcatc gccggggagc
     1861 gcgagcgcga aggcgtgctg ctctgcctgg acgcgaaggg ccggcgcctc tgggagcgcc
     1921 cgctgaacct gggccccggc ccctatgcgc tggcgcccct gccccgcgcg gtggtggtga
     1981 cgagcgcgtc cggcgccgcc acgcgggtgg cggcctccgg cacggtggac tggcgcgtgg
     2041 gcgcggcggg cgagccgctc atcggcgcgc tcccggccca caccgcccgg gacgtcacgc
     2101 tggtgccggg tgagcgggtc cgcgcggtgg acccccgcgg cgggcaggtg ctcgcggagg
     2161 tccaggccgg cgtggggctc gtcgcgctcc aggccgacgt gcgcctcaac ctctacttcc
     2221 tggacgacgc cggcacgctg tccgcgtacc ggctcggctc tcacttcacg gtggtggagt
     2281 agccgccgcg cgggctcaat cccgcgcggc cttcgacggg tcgatctcct cctcggggcg
     2341 ctcctcctgg gtggcgccct cccacgactc gccggcgccc agcttccaat ccgaggggac
     2401 ctcgtccacc ttccacacgt aggtggacgt cgcggtgccc cccgaggccg cgctggaggt
     2461 gaggttgatg gtcatctcca tcttcgacac ctcgttcggc agcaggtcca ggtcgtagtg
     2521 gcccagcacc atctgcgggg ggaacgcctt gatgaagcgc tccgggtggt caatcttcat
     2581 ggacgggtcg gcgccgcgca tctcgcccag cagcttcccc ttcgcgtcgg tgagcttcca
     2641 caccgtgtcg aacgcggcgc tggtcaccat cttcagcacc ttgccgtcgt ccttgagcgt
     2701 gcgctgcgcg ccccacaccg cgagctgaag ccggatctgg ggcttgccca gcaccatcac
     2761 cacgtcggag gagatgaggt ccaggcgcat gcccttgtcc gtcgccgtcc acaccgggcg
     2821 gtagcggccc tcgcgcatgc tgtcgaacgt cttgcgcgcg tactcgtaga aggccttgcg
     2881 gtcattggcg cggtcatcct tcgcgccgcg ctcctcccgc tgctccagct ccaggaggta
     2941 gtcgtcgaac ttcgtgttcc cctggaagcg cgtcaggtgg tcgtccacct gctcgaaata
     3001 gttcttgaag aagggcttca gctcgtcgcg gtagctggcc tcatccgggt tggagcgcat
     3061 ccagccgacc cgctccaggt actccgcatg gattcgctgt tgatctgctt cccgctgcgc
     3121 ctcgagcgcg cggccactgg cgctgcggta gctcatgacg gcgcccagca gcacccccgc
     3181 gacgatgacg atgactccga agtagcgctt cacgcggcag tctcctgtgc ctttgtcctg
     3241 gcagtgatat gaaaattccc caacgtgatc caggccaccg ggacccacct gttcaccacg
     3301 ccccttccct tggaggaagg agagctgctg ggcaaccccc aggtggcctg ggccgcttat
     3361 ggagaaccat cagacggcaa agcggtggtg ctgctgcacg acctcgcgca ctcgcacctc
     3421 gcgttgggcc cgccggagga ctcggcgtac cagccttcgg ggtgggggcg cgcgctggtg
     3481 gggacgaacc tcgcgttgga tccggccgtc acgccggtcc tcgcgcccgg gctgctgggc
     3541 agccccttcg gcagcacgtc ccccgcgacg gtgaaccccg cgtcgggcga gcgctggggg
     3601 ctggagctgc cgccgctcac cgtgcaggac atggcgcgcg ccgtgtcagc cacgctgcgc
     3661 gcatgcgggc tgagccacgt gcgcgcgctg gtgggcgtgg gcctgggcgg ccacgtggcg
     3721 ctgcggctgg cgtccctctt cccggacctg gcggacggcg tggtgaccct tggcgcggcg
     3781 cgcgcgctgc cggaggccgt ccgcgagaag ctgggcctgt cctggcaggt gctgcgcgcg
     3841 gacccggact accgcgacgg cctctacgcg ctggacgcgc cgccgcgcaa gacgatgcgc
     3901 aagctgcgcc tggacttcca gaagctgctg gccggacgcg agcacctcac cgccacctac
     3961 cgtgacgccg agtccgcccg cgccgcgctg gacgccgagg cggacgcgtt cgccgacgcc
     4021 ttcgacgcgg tgtcctgggc gtcgctgtgc tcggcgtacg cgggcagtga cgtgtcggac
     4081 ggcttcgcgc ggatccgctc gcgcgtgctc ctcatcgcgg gggcgtcgga cgccatggcg
     4141 ccggtgaacc gggtgcggga cacctatcac ctgctgagcg ccgcgggggt gagcgcccgc
     4201 ttcctggagc tgccggggcc cggggaccat gcggcgctgc tccaggattc ggagcggctc
     4261 catggcccgc tgagcgactt cctgcggcgg tgctgactag tcccgctgcg cgaccccgga
     4321 gagcttgccg cgcagcgcgc ccaccagcat cccgatggcg atctcgttgt tgccgccgtg
     4381 cgggatgatg atgtccgcga agtgcttgga cggctccacg aagcccatgt gcatgggccg
     4441 cacgtggcgc aggtactggc tcaccacgtg gtcgaagtcg cggccgcggt ccttgatgtc
     4501 gcgggtgagg cgccggagga tgcggaggtc gtcgtccgcg tcgacgtaga tcttcacgtc
     4561 catctcatcc cggacctcct tcatgtggag gaccaggatg ccctcgatga ggatgatgtc
     4621 gccgggctcc acgttcttcg tctgcggctg acgggacgag gtgacgaagt cgtagacggg
     4681 cttctggatg ggctgcccgg acttcagcgc cttcaggtgc ttgaccagca gctccctgtc
     4741 gaaggcatcc gggtggtcga agttcacctc gcgccggtcc gccagcggca ggtccttcag
     4801 gtcccggtaa tacgaatcct gatcgatgaa ggccacacga caatcggcca atgcctcgcg
     4861 aaccttgcgg gcaacggtgg tcttgccgga tgcggtaccg cccgcgattc cgacgacgag
     4921 gggggacgcc atggcggcgc gactatctta tccaggccgg ccagcgcaag ggttcggtcc
     4981 aatcccggac atttcttgtc ctatccacgg cggatcagcc cacgctgcct tgcctcctcc
     5041 acgagcccgg gcgccacctc ggcctcggtc aacggctgga cagcgagcat tctcaggaat
     5101 tcacccatag ctggtgacac cgtcgtgacc tccagcccgc ctgccctgcg ccgcgcggtg
     5161 aaggaccagg ggccgggccc cgcccttcgc gccacctggt tcagcgactc caggccggac
     5221 agctccagca cgccagacgc ccacgcacgc gcggtgaggt gacggcgcac ggctcgggcg
     5281 gcatggacca gctcggacag gtccacgggg agcgacccgg cccgcacgtc ctccatcagc
     5341 cccacctgcc ccggcgcggg cgcgggcacc cggcgcagga agagcgaggg cagcaacatc
     5401 tccagggtca acgccgccga gcccccctcg tccggcgact cgcgcaggcg ccgcatggcc
     5461 cacgaggcga aggcctgccc cggcgagcgg cccaggggac tgaagagctt ccggaactcc
     5521 ttcgagccgg tgaaggccgc gagcgcctcc cgcgtgcccg cgagcagcaa ccgggtgagg
     5581 ggccagtcgc gatccaacac ctccgcgacc accgccaggc ggaagtcctg gtccgccagc
     5641 gtgccggcct cgtcctccac ggaggtcacg ggcgcggccc catagccggc gtcgaacaac
     5701 gcccagggct ggctgtcctc cgtcagcgct ggcacgggcg cggcgctggc ctccgggacg
     5761 cgccacgtca gcggcggccg cgcgccggcg ggcaccagct cgcgcaggcg gcgcaaggag
     5821 gccgcggcca cctcgggcgg gtggccgtcc ccctcgaagg tgacggcgcg cagcgacggg
     5881 cagcggggaa tcagcgaggc cagcagctcg aacagctcct cgcgcaccgg ctgcgtgtgg
     5941 tcgtccacgt agaagcggcg cggcccccgg tgcgtgacga cgcctccggc gatgtgcagc
     6001 tcgatgacct ggtcgagcgg aaagccctcc agccctgtct ccagcggcag gccggcggag
     6061 agctggtggc tgaacaggtg ccccaggtcg agcagcagcg gcgcgccggt gcgcgcgtgc
     6121 agcttcgcca tgaagtccag cacgtgccac tgcccgcgcc gcgccagcac ggccgggttc
     6181 tccagcacca gcggcatgga caggtgccgc tggacgtgga gcgcgtgcgc cacgctggcc
     6241 tccaggcccg cttcattgaa cggcggcgtg aagtagaggt agcccgggaa gggctgaccc
     6301 tccacgtgcc accagcccac gtcgttgccc acccacgggc tctccacggc gcgcgcgtgg
     6361 gcgtccagct ccaccagggc ccgtgcgggt tccaggccgg gtccccagag gttgaggtgg
     6421 acggggtgga acaacaccgg gacgtcctgg cggcggcgcc acatctccgg gaacagcgag
     6481 gcgtgcgcct tggattcttc caaggagagc ggcgcgctgt attccacgta gtcgaagagc
     6541 cccggcgacg cgtccagcaa ccggtagggc tgcggcacgt cggccatgct caggttgctg
     6601 ctcagcccca ggcctcgcca gggcagtgtc caactcgggg gcgccgcgtg cgtcatctcc
     6661 tcaacgtaga cgcgccccgc ggccaccgcc acacacgact gcctccgagt gcaacgctgg
     6721 aggcccgcgg catggggtac gctcgccgat tcccccaatc ccaggagccc cgatgccccc
     6781 tgcctcacgc gccgagattg acgaatccaa cgcgcagtac cgtggagcct ggaggctgtt
     6841 cgccctgggc agcaaggccg gcgaggtggt ggagcgcccg gacgtctaca tcaccgcctg
     6901 caacgtcgcc tggtccatga tgaacgccgc cttcctgcgc gcgccggtcg aaacggagca
     6961 ggcgctcgcg gccgcggcgg cctccgcggc gcgctacttt tccgcgggca agcatggctg
     7021 gagcttcctc atctgcgacg actggctcgc ccccgccgtg cgcgacaacg cgccgtccat
     7081 cctcgactgg tatgggctca agcccgagct ggtcgtcacc ggaatggtcg ccgaccgcct
     7141 gcaaccctcc ctccatcccc catcgccgtt cgacctccgc cccgtgacgg atgccggggg
     7201 acgccaggcc gtggccgaca tcaacgccct ctcctacgac gtcccccaac acctcggccg
     7261 cgaggcgttc gacgaggcca ccctctacaa cgcggactgc cagggcttcg tcgcctgccg
     7321 cgacgaggag cccgccgcca gcaccgtggt gctgcgcgtg gacgccgccg cctacgtcgc
     7381 cctggtcgcc acgcatcccc agcaccgccg ccagggcgcc gccgaggccg tgatgcggca
     7441 cgccctggcc agggcccgcc aggactgggg caccgagcgc accgtcctcc acgccacgga
     7501 ggcgggccag cccgtctaca cccgcctggg ctaccggccc gtgacgcgct tccgcatgta
     7561 cctggcgcca ccgccgggac agggctgaga tgccgtcagg gccaggcggg ggagtagaac
     7621 cagactccca ttcgcgacgc gctgtagaat gccgctcctg catgaactgc ggcccacatt
     7681 cttcgacggc gcggaggtta tggcagactg atccgtgagg gggcttgccg cacgtcttgc
     7741 atcgcccagc cacacggagt ccgcacatgg ccaagtggga tgatctcgtc ctggcgattc
     7801 caccgcatgc gggcgggagg ctcgaagtgc ccgccccggc ggcgctgctg gagacactgc
     7861 tcggcctcca caacgtccag ctcaccctcc tccgcgggtt gcgccgcgag ctccggccgc
     7921 ttcaggaagg ccccttccac gccggccgga cgcacctgga ggccgcgcgc gtcgcccacg
     7981 ccagccgcga cgaacagctc gaacggctga aggccgcccg gggctgcttc accgaggcca
     8041 tgggcatgga gcgagacaag gcgcggctgt ccttcatcgc cctgcacctg ggcctctgct
     8101 ggctgctgct gggcgcggag cgggacgcgc gggagtggac gcagctcgcg caccgcaccg
     8161 ccgtggaggc catgaaggac atcctggttg acgcctcgaa gcaccagggc ctcacgcgca
     8221 accggtacct ggctccggcc atcaccttct tcatgttctt caccagcgcg gccacgctgg
     8281 gcgccggcta cctcttctgg gaccgcgtgc tcagcaagcg caccggcacg gtgatgcggc
     8341 gcgcgctggt gcagatgcag tccctggcgg actacgtgga cgcgctgggg gagatccgcc
     8401 tccgcctggg agagctctcc catgacgtcg cccggtacga gctgacccac ggcgtggacg
     8461 agcagcagtt cgtcgcccgc atcaccatcc ggaagctcat caacgaccgg gaggccatct
     8521 acttcaacgg ccgggagacc cggaaggtca cctggaacga ccaggagcgc tccgaggtca
     8581 tcacccgcga gctgctctga cggcccgcgc cgtcaccgcc ctgtcaaatc ctgttttcag
     8641 gattgccgcg agcgaaacgt cagccccccc cgccatagta gcggcacttc cttgatgggg
     8701 gcgaaggtga cggcgcagac gatgctcgtg ttcattccag tgattctcct actcgccgcg
     8761 gcggttgtga ttccgcggcc ccgccggcgt tcacgccggt agtcgcagtc gcaggcacgg
     8821 ccggggcgca tccccggccg tctccccggc ccggatgggg gtgtgtagag tgcggccgtt
     8881 gtcgcaaccc tcccctcccg agccgccgtg gccgaacgtc ccacagtcct ggtcgtcgac
     8941 gatgatccac acctgaggga gatcgtccgc ttcgcgctgg agcagggagg cttccgggtg
     9001 gacgaggcca cggacggccg cgccgccctg gcgcaggtcg accgggccct cccggcgctc
     9061 atcgtcctgg acatcatgat gccggagctg gacgggctcg ccgtgtgccg ggaggtccgg
     9121 cgcaagcatg agctgcccat cgtcttcctc tcctcgcgcg acgacgaggt cgaccgcatc
     9181 ctcggcctgg agctgggcgg cgacgactac ctcaccaagc ccttcagccc gcgcgagctg
     9241 gtggcccgcg tgaaggcggt gctgcggcgc gcccggcccg cgccggaagc cgaggcgccc
     9301 cccgcgagga tgcagtcgcg gggcccgctg cgcatggacg cggagcgctg gcgcgcgtgg
     9361 tggcgggaca cggaggtggt gctcacggtg acggagttcc agctcctggc cacgctgctc
     9421 cgcgtgccgg gcaaggtctt cacccgcgac gagctgatga accgcgtcta cgacgacgtg
     9481 gtggtcagcg accggaccat cgacagccac gtgcgccgcg tccggcagaa gttcgcgagc
     9541 gcgggcggag acgtcatcga gacggtgcat ggccttggct atcggctcgc cctcccctga
     9601 gcgccgcgcg cgcccgcgcc tgtggctggt gttcgccgcg gtgggcgtgg cggggttcgc
     9661 gctcacgctc gcggggctcg ccttcgtgcg cgtctacgac aaccagctca tccgccagac
     9721 ggagtcggag ctgatcgccc agggcgcggt catcgccgaa gtcttccgcg agcgcctgcg
     9781 cgacgccgag cccagggacg accatggccg ggagcgcacc gccccctggc ccttccccct
     9841 ccccgacgac acgcgcctgc gccccattct cccctccctg agggcctcgg atgccacgct
     9901 cccctcgggg ggcacgcccc agccctcgcg cgtccccgcg gagccgctgg cacacgccgc
     9961 gggactgcga ctgacgccct tgctggagca ggtgcgcgcg gcgacgctgg ccggcatccg
    10021 cgtggtggac ccggagggcg tggtggtggc cagcagcagc gcctcgctgc tcgggaccac
    10081 gctggcggac cgcacggagg tccaacgggc gctgcgcggc gagcccgtca gcgtgctgcg
    10141 ccgccgcgtc gccgagcccg aggacacccc actggcctcg ctcagccgag acaccggaat
    10201 ccgcgtgtcg gtggcgctgc ccgtgctgga cggcgagcgt gtctggggcg cggtggtgct
    10261 gacgcgcacg cccatgacat tcgccaaggc gacgtacgcg gaccggtgga acctcaccgc
    10321 caccggcttc gtgttgctgg gcgtggtggc gttgatgtcg ctggccgccg ccgcgctggt
    10381 gggccggcca gtgcgcgcgc tgatgcgcca gacgcgcgcc atcgcgctga gcgacccctc
    10441 gggcttcgaa cccgtggccc gccccgtggt ggccgagctg gccgagctct ccgagtccct
    10501 ggccggaatg gccaccgcgc tgcgggacag gaaccagtac atccgctcct tcgcggcgaa
    10561 cgtgtcccac gagttcaaga cgccgctggc ctccatccag ggcgcggtgg agctgctgcg
    10621 agacagcgcc gacgcgatgt ctcccgaaca gcgcgcccgc ttcctcgcca acgtggacgc
    10681 ggatgcccgc cggctcaccc gcctggtgca gcgcctgctg gagctggccc gggcggactc
    10741 catgacggcc acgcccgcgc aggtggcgct gggcccgctg ctggaggggc tcgccctccg
    10801 cgcccgcgcc gaaggtgccc tggacatcca cgtgacgccg ccacccgcgg gcctgacgat
    10861 ggggctcccg caggaggtgc tcgacgacgt gctgtggcag ctcgtcacca atgcccgcca
    10921 gcacggcggg gacaccgtcc gcgtggacct gtcggtggag caggccccgg aggcggacct
    10981 ggtgcgcgtg gtggtgaagg acagcggccg cggcatctcc gaggccaacc gggcccgcat
    11041 cttcgacgcc ttcttcacca ccgcccgcgc gcgcgggggc acgggcctgg gcctgacgat
    11101 tgcccagtcc atgctgcgcg ccttcaacgc gaggctggag ctcctcccct ccgccacggg
    11161 caccaccttc gcggtggtgg cgcccgtgcg cgggcttcag cggaagtagc tgtcggagat
    11221 gtccacggag taccccagct ccagcgtggg cgcggacgcc gccaccagga actggcggaa
    11281 gtgcagcgcc ttgccgggca ggcccgacgt ctccggcgtc gcgctctccg gagactcata
    11341 ctgctccagg ctgccgccct cccgccggta gttgaacacc gcggggaagt gcgccttggc
    11401 gcgcagctcc gccacgggcc aggacacgcg cggcagcgcc agcttcagcc gcccggagag
    11461 gtggaagacg cccagctcgc gcgacagcac cacctccagc gtgccgcccg tctcccccag
    11521 ccgcgccggg gcggccttca acgtcagcgt gccctcctgc gactccacgg gcacggggat
    11581 ggcgttgagg gtgacgcgct ccagccggtg gccctccggc agcaccagct ccagcggctg
    11641 ttcgcggtcc aggctcagcg cgtagctgac gcgcaacgtg gcgcggccct cc
//
