LOCUS       NZ_CP022098            11491 bp    DNA     circular CON 17-FEB-2024
DEFINITION  Cystobacter fuscus strain DSM 52655 chromosome, complete genome.
ACCESSION   NZ_CP022098
VERSION     NZ_CP022098.1
KEYWORDS    RefSeq.
SOURCE      Cystobacter fuscus
  ORGANISM  Cystobacter fuscus
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Archangiaceae; Cystobacter.
REFERENCE   1  (bases 1 to 12349744)
  AUTHORS   Rupp,O., Goesmann,A. and Sogaard-Andersen,L.
  TITLE     Sequencing and comparative analysis of myxobacterial genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12349744)
  AUTHORS   Bruckskotten,M., Treuner-Lange,A., Rupp,O., Goesmann,A. and
            Sogaard-Andersen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUN-2017) Department of Ecophysiology, Max Planck
            Institute for Terrestrial Microbiology, Karl von Frisch Str. 10,
            Marburg, Hessen 35043, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2016
            Assembly Method                   :: Celera Assembler v. 8.1
            Expected Final Version            :: yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 10:04:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,093
            CDSs (total)                      :: 10,016
            Genes (coding)                    :: 9,906
            CDSs (with protein)               :: 9,906
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 26 of 110
            Pseudo Genes (incomplete)         :: 101 of 110
            Pseudo Genes (internal stop)      :: 1 of 110
            Pseudo Genes (multiple problems)  :: 17 of 110
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:24:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 10964011
            Orig. end                         :: 10975502
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11491
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="54"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11491
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11491
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6491](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6491
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(490..1230)
                     /gene="nagB"
                     /locus_tag="CYFUS_RS44435"
                     /old_locus_tag="CYFUS_008785"
     CDS             complement(490..1230)
                     /EC_number="3.5.99.6"
                     /GO_function="GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA]"
                     /GO_process="GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611360.1"
                     /locus_tag="CYFUS_RS44435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008785"
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="WP_095990738.1"
                     /transl_table=11
                     /translation="MNVRVFSSEVEAAAACAAHVAAAVREQPSLVLGLPTGRSPLNVYR
                     QMVALHQRGALDFSRVTTFNLDEFLGLPPDDPCSLRAYMDRHLFQHVNLAPERVHFLDG
                     SAEKAQWECARYDAALEAAGGIDLLLLGIGSNGHVAFNEPGDSLQAASHRARLSRETRQ
                     AMTPMFGDEISRVPMAALTLGMGALFKARRVILLAFGVNKAAAVTAMVRGPITSHCPAS
                     LLQLHGQVEVWVDPDAGHGLEGSG"
     gene            1439..2476
                     /locus_tag="CYFUS_RS44440"
                     /old_locus_tag="CYFUS_008786"
     CDS             1439..2476
                     /EC_number="2.7.1.11"
                     /GO_function="GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556305.1"
                     /locus_tag="CYFUS_RS44440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008786"
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="WP_095990739.1"
                     /transl_table=11
                     /translation="MKVAVLTGGGDCPGLNAVIRAVVRRASAHGFEMMGLRDGWKGLLD
                     DNHFRLTRETTSGILHRGGTILGTSRVNPFKVENGLERVKRAIERNDIHAVIAIGGEGT
                     LSAATRMSQEGVRIVGVPKTIDNDLNGTDFTFGFDTAVGIATEAIDRLHSTAESHKRVI
                     VCEVMGRHVGWIATYAGLAGGADVILVPEVPADLERVAEHIKHRHASGRTFSIVVVAEG
                     TRIKLSHEGQEQLITSGALDEAGRPRLGGVGALLANEIERRTGYETRVSVLGHIQRGGA
                     PTAHDRVLATRYGVHACDMVAQGEFGKMAALRGNEIVSVDLSEATRELKKVPQEFFQVA
                     QVFFG"
     gene            complement(2478..3530)
                     /locus_tag="CYFUS_RS44445"
                     /old_locus_tag="CYFUS_008787"
     CDS             complement(2478..3530)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF023304.4"
                     /locus_tag="CYFUS_RS44445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008787"
                     /product="type VI immunity family protein"
                     /protein_id="WP_332468323.1"
                     /transl_table=11
                     /translation="MATDYPRFRLRGIPEVRVLMCPGGIQRPERRLLARDVFRIVFYMP
                     HDHPDIAHGVSHAIDCYMRAVGEGPKTINYVSRSYDEGSALTAEQWGWARLLLQNTRRW
                     SFPDDYEDWAQREIEKRRFERGLLFTGGSGNRNGYEFEYKARIPWRPAPETTFVSLLTA
                     TLPIEFLERHGPGPVRRLVLDMASQLRFASGHAGLALRLYWWLPRADEALRIGPLRYPG
                     LDLRDAWRHENQMGFQVDGVHWLNFLGSPVLTQLGGAEALRSRLRSAGTTVVPLDDERA
                     VVSLGERPEVGDLSAGKTLPAYRELAQVLEPWLEPLFLGPSGDIAGETRYTSLLLTEGE
                     ARRWWRRFLD"
     misc_feature    complement(2931..2933)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3963..4895
                     /locus_tag="CYFUS_RS44450"
                     /old_locus_tag="CYFUS_008788"
     CDS             3963..4895
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622759.1"
                     /locus_tag="CYFUS_RS44450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008788"
                     /product="HD domain-containing protein"
                     /protein_id="WP_095990741.1"
                     /transl_table=11
                     /translation="MENPALEMAPVPHRLAGLRSLVSELMILKRVRTPEQPDGLAAHGF
                     RRAWAALVSGEDPATLALRETALALSAVQLGGMDFDVLRRVGLSPEQILGVLRRGFEAA
                     TASVDEGLRDRLRTALAEASTWVGAEPPAFVEHLVRQPRAGPPHANTQRVALLPAESHA
                     DHCYVVAVASVLVSPLFGADPTGPFVAALSHHLANAVLPDTGGHGNQLLGEHLAPLMAR
                     ITEQVLDTLPSELAHRVREARRLLSHIDTPEARAFHAANTLDRVLETDAHARAAGFTPR
                     QALEDLEFTHDEPIRAYENAVLAAVGLSH"
     gene            5001..6491
                     /locus_tag="CYFUS_RS44455"
                     /old_locus_tag="CYFUS_008789"
     CDS             5001..6491
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622758.1"
                     /locus_tag="CYFUS_RS44455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008789"
                     /product="YcaO-like family protein"
                     /protein_id="WP_095990742.1"
                     /sec_met_domain="YcaO (E-value: 1.1e-55, bitscore: 188.8,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAKKSQQEACEAYRAAFPPGELEMFRADPIDRVGIPAVAASLRL
                     KTGTQFATHGHGTTPEEAEASALGKLAESVFTETSLRAHARERGTYRELARTRGPSGVV
                     DPLKLCLPAGSPYHADMELTWVTVTRLTTGEKVLLPEEWVATRSGQLQGRTPLISPITH
                     GQGAGLTHEQALTHGLLELFERDGNGLRTRALDQGVVIDLKDAALGPELQSLLAHCERL
                     GIEIIPKLACTDFGLVGLYVVGRDPALRDQPMALTACGEAADLDRERALHRALLEYAGS
                     RTRKAFANGPLELAEKIAPPGYMDRFLPYLELEDEEPRALQAMADWAQLSAAQMRSLME
                     QRVLAERGRMPFASLPTTPNPGDARQRADVLVRKLTQAGFDILVADMSPTNHSVVSLKV
                     IVPGLEAETMSDHRIGERGVAKLLARQDPLAGLGKPPEGAQRVRLTPEAEERLGGPTWF
                     HSRLAEAIVGRLYALYREPERHTAQLMPRQRRYGGDPS"
     gene            6599..7435
                     /locus_tag="CYFUS_RS44460"
                     /old_locus_tag="CYFUS_008790"
     CDS             6599..7435
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622757.1"
                     /locus_tag="CYFUS_RS44460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008790"
                     /product="aldose epimerase"
                     /protein_id="WP_095990743.1"
                     /transl_table=11
                     /translation="MQEEDTVLLKDGDCVAEIVPERGALVSRFTVGGEELLFLDASTLA
                     DPGKNVRGGIPVLFPSPGVLPGGTYPAEGRDYTMRRHGFARDLAWEVRHRENARTELVL
                     GHSDQTLREFPWRFEARLTVTLSGGALRLGFAAENRDTRPMPLHLGYHPYFHVPQANKA
                     VTRLDTDATRAWDNRTGAPVTFSGLDLTSAEVDMHLLDHSRPGTTLHRGPGLRPVVLSW
                     SESFTTVVVWTLTGRDFVCVEPWTAPGGALRTGEGLLSVAPGATFSSEFEIRAGRE"
     gene            7445..8653
                     /locus_tag="CYFUS_RS44465"
                     /old_locus_tag="CYFUS_008791"
     CDS             7445..8653
                     /EC_number="4.2.3.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622756.1"
                     /locus_tag="CYFUS_RS44465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008791"
                     /product="threonine synthase"
                     /protein_id="WP_095990744.1"
                     /sec_met_domain="PALP (E-value: 1.7e-65, bitscore: 220.5,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSFLSHLECSRCHQTHDADRLQNLCTCGGPLLVRYDLKAAARAV
                     RPTNLAGRVSSLWRYRELLPLRDDKHLITLGEGMTPLFPLPRLGASVGLPDLWLKDEGL
                     NPTASFKARGAATGVSRAKELGVTALAMPTNGNAGGAWASYGARAGISVTLVMPTDAPA
                     MSVLEATAVGAQAYMVRGQITDAGAIVGRSARAHGWFEAATLKEPYRIEGKKTMGYEIA
                     EQLGWSLPDVILYPTGGGVGIIGIYKALLEMRELGWLPDSVRLPKLVAVQAEGCQPIVK
                     AFREGKDVSEKWENASTVAQGIRVPKALGDFLVLQAVRETGGTCVAVSDADTLWGLEQI
                     SRQEGAFICPEGAALVGAARQLLREGWLDAGQRVLLLNTGAGIKYPDVMTPKLPILELN
                     ATL"
     gene            8994..9236
                     /locus_tag="CYFUS_RS44470"
                     /old_locus_tag="CYFUS_008792"
     CDS             8994..9236
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CYFUS_RS44470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CYFUS_008792"
                     /product="hypothetical protein"
                     /protein_id="WP_095990745.1"
                     /transl_table=11
                     /translation="MQLRTMVWMRVALSALALGGTACGSLDLSCQRDQDCLDSELCHPD
                     EQECVRRCSTNLDCYEARPKCQEMGSSNSTRICKA"
     gene            9461..9991
                     /locus_tag="CYFUS_RS44475"
                     /old_locus_tag="CYFUS_008793"
     CDS             9461..9991
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CYFUS_RS44475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CYFUS_008793"
                     /product="response regulator"
                     /protein_id="WP_157759004.1"
                     /transl_table=11
                     /translation="MRPPIASSGPRAEGQGQRRILVVDDDPAILKVWRRVLGTRTAPSS
                     LDHLEKKLFGATPPAAPTDAGFAIDTASQGMEGYQKVVSALEEDRPYTFALVDMRMPPG
                     WDGLETILHMLEKDPRLEVAICSAFSDISRDEVAQRVGRSDLLWIRKPFELEAARDLAR
                     KLSEQGVHRRQTR"
     gene            complement(10000..11220)
                     /locus_tag="CYFUS_RS44480"
                     /old_locus_tag="CYFUS_008794"
     CDS             complement(10000..11220)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CYFUS_RS44480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CYFUS_008794"
                     /product="hypothetical protein"
                     /protein_id="WP_095990747.1"
                     /transl_table=11
                     /translation="MSPRNFVKQALPWVALVLAIAALGYTWRWALSKSSDRQRMHHAFS
                     TGTTDYIFSLCPSTPLAQSEPRWASDMRGRCVVHQPTWGTAQQGGARPELDIEKGSTWQ
                     SLQLLSRLVLEISPGKMLSLLSLVVISLAGTLGVQRKGWLERQGLSLSITRGAAVSMLT
                     VVLVALSVSLVLLDEQETLSMCQVTSGTYANLLSVDTRCSQQTMLLRETQEKLEQKKGE
                     LDARAQELEELKREIASLGVMRDALGSFKTELQSQSESTQQLIRAVTGATLEGINKALD
                     PIHADVKGLDTSLKTDFKKGLRGELQEDMTSLVRAELKTSLQGEQLQELIRREVRAALR
                     EDLREVVRGELRESVKAVLAASPAVLPPAPKPETKTPARTNVTAASSKPAAAPGRKPSP
                     PPAPSKR"
ORIGIN
        1 tgcaggtgga gttcgacccg gaggccgtgc gcggctaccg gctgctcggc tacgagaacc
       61 gcgccatcgc cgaccgggac ttccgcgacg acaaggtgga tgccggggag attggcgccg
      121 gccacaccgt caccgccctg tacgaggtgg atctcacggg cgagggctcg cgcgtggcca
      181 ccgtgcacgt gcgctccaag cggcccggtg gcgtggaggc cgccgagcag accttccccc
      241 tggagcgcgc gagcctgcac gcccacctgg acgaggcctc ctccaacctg cgcttcgccg
      301 ccgccgtggc gggcaccgcg gacatcctcc ggggcgctcc cgaggcccgg gaatggagcc
      361 tcgccaccgc cgagtccctc gccgaggagt ccgtggacgg ccagccggac cgctccgagt
      421 tcctcggcct gctgcgccag gtgcgcgagt ggcgcaccgc gtccgtcgcc gcgagctacc
      481 cggactgagt cagcccgagc cctccaggcc gtgcccggcg tcgggatcca cccacacctc
      541 cacctgcccg tgcagttgga gcagggaggc cgggcagtgc gaggtgatgg ggccgcgcac
      601 catggccgtc accgccgccg ccttgttcac cccgaaggcc agcaggatga cgcggcgcgc
      661 cttgaagagg gcccccatgc ccagcgtgag cgccgccatg ggcacccggg agatctcatc
      721 cccgaacatg ggcgtcatgg cctgacgcgt ctcgcgcgac agcctcgccc gatggctcgc
      781 cgcctggaga ctgtcccccg gctcgttgaa ggccacgtgc ccgttggagc cgatgcccag
      841 cagcagcaga tcgattcccc ccgccgcctc cagggcggcg tcgtagcggg cgcactccca
      901 ctgggccttc tccgcgctgc cgtccaggaa gtgcacgcgc tccggggcca ggttcacgtg
      961 ctggaagaga tggcggtcca tgtaggcgcg cagactgcat ggatcgtccg gaggcaggcc
     1021 gaggaactcg tccaggttga aggtcgtcac ccgggagaag tccagcgccc cgcgctgatg
     1081 gagcgccacc atctgccggt acacgttgag ggggctgcgt cccgtgggca gaccgagcac
     1141 cagggaaggc tgctcacgga cggccgccgc gacgtgggcg gcacaggcgg cggcagcttc
     1201 cacttccgag gagaagacgc ggacgttcaa ggaggaagaa ccgtggcctg gggaaggtct
     1261 ccagcatagc gcccaccgag gcccccgtca cccatcgcat cgggcggggc gccctcggca
     1321 aggtgggtag ggtcggcccg gggaccgccc cccgggaccg gtggcccccc ctcgaacggg
     1381 cgaattcacc tcaacttcga ggatgactcg cgcgggaggt gagctagaag tcgcgttcat
     1441 gaaagtcgcc gttctcacgg gcgggggtga ttgccccggc ctcaacgcgg tcatccgcgc
     1501 tgtcgttcgc cgcgcgagcg cccatggttt cgagatgatg ggcctgcgcg atgggtggaa
     1561 gggtctgctc gacgacaacc acttccggct cacccgggag accacctccg gcatcctcca
     1621 ccggggtggc accatcctgg gcacctcccg cgtcaacccg ttcaaggtcg agaacgggct
     1681 ggagcgcgtc aagcgcgcca tcgagcgcaa tgacatccac gccgtcatcg ccattggcgg
     1741 tgaggggacg ctgtcggccg ccacgcgcat gtcccaggag ggtgtgcgca tcgtgggcgt
     1801 gcccaagacg atcgacaacg atctgaacgg cacggacttc accttcggct tcgacaccgc
     1861 ggtgggcatc gccaccgagg cgattgatcg gctgcactcc accgccgagt cgcacaagcg
     1921 cgtcatcgtg tgcgaggtga tgggccggca cgtggggtgg atcgccacgt acgcgggcct
     1981 ggccggcggc gcggacgtga tcctcgtgcc cgaggtgccc gcggacctgg agcgcgtggc
     2041 cgagcacatc aagcaccgcc acgcctccgg gcgtaccttc tccatcgtcg tggtggccga
     2101 gggcacgcgc atcaagctgt cgcacgaggg ccaggagcag ctcatcacct cgggcgcgct
     2161 ggacgaggcg ggtcggcccc ggctgggagg cgtgggcgcc ctgctggcca atgaaatcga
     2221 gcggcgcacg ggctacgaga cgcgcgtgtc ggtgctcggc cacatccagc gcggcggcgc
     2281 gcccacggcg cacgaccggg tgctcgccac gcgctacggc gtgcacgcct gcgacatggt
     2341 ggcccagggc gagttcggca agatggccgc cctgcggggc aatgagatcg tcagcgtgga
     2401 cctgtccgag gccacgcgcg agctcaagaa ggtgccgcag gagttcttcc aggtcgccca
     2461 ggtgttcttc ggctagctca gtcgaggaaa cgtctccacc aacgccgcgc ctcgccctcg
     2521 gtgagcagga gcgaggtgta ccgcgtctct ccggcgatgt ccccggaggg gccgaggaac
     2581 aacggctcga gccagggctc cagcacctgg gcgagttcac gatacgcggg aagggtcttc
     2641 cccgcggaca ggtctcccac ctcgggccgc tcgcccaggg agaccacggc gcgctcgtcg
     2701 tcgagcggga ccacggtcgt cccggccgac cgcaatcggg agcgcagggc ctccgcgccg
     2761 ccgagctggg tgaggacggg ggagccgagg aagttgagcc agtggacacc gtccacctga
     2821 aagcccattt gattctcgtg acgccaggca tcgcggagat ccagacccgg gtagcgcagg
     2881 ggcccgatgc gcagggcctc gtccgcccgt ggaagccacc aatagagccg taaggccagg
     2941 cccgcgtgcc ccgaggcgaa acggagctgg gaggccatgt cgagcaccaa ccgacgcacg
     3001 ggccccggcc catgccgctc gaggaattcg atggggagcg tcgccgtgag caggctcacg
     3061 aaggtcgttt cgggagcggg ccgccacgga atacgggcct tgtattcgaa ctcgtagcca
     3121 ttccggttgc cagagccccc cgtgaagaga agcccccgct cgaagcgtcg cttctcgatt
     3181 tccctctgcg cccagtcttc ataatcatcc gggaaggacc agcgcctagt gttctggaga
     3241 agaaggcgcg cccatcccca ttgctccgcg gtcaaggcgg agccctcgtc ataggaccgg
     3301 ctcacgtaat tgatggtctt gggcccctcc cccacggctc gcatgtaaca atcgatggca
     3361 tgactcaccc catgcgcgat gtcagggtga tcgtgaggca tgtagaaaac aatacgaaac
     3421 acatcacgcg cgagcagtcg ccgctcgggg cgctgaatcc ctccagggca catcaacacc
     3481 cggacctcgg gaatgcctcg gaggcggaat cgggggtaat cggtcgccat cggttgagag
     3541 tacccaccgt tgagtgaccc actccaggac gaccagggtt ggatgcgaaa ggccaccgcc
     3601 tccgtgccgt ccgcttccac accttggaac caccccgcgg gacaggatga gaggagccct
     3661 tgtttgactt tcagacccgg acctcgtctc gaggagccgt attggtttcc agggcttgtg
     3721 acataacgaa gacgtgccaa atcattgccc acgcccccgg gaacgaccca gcgatgggga
     3781 accacctgaa cgcacctcgc ctcgaaagcg tggcgcccgt tccggtcagg gaagctcacg
     3841 ggcggtcgtg cagtgaccac agggggaaca ggcacatgtc tgggatggag cgtctgggag
     3901 tcgcgtgggt cggtctgggt ggagccgtgg tgacggcggc cctggcgggg atgggctagg
     3961 ccatggagaa ccccgcgctg gaaatggccc cggttcccca ccgcctggcc ggactgcgct
     4021 ccctcgtgag cgagctgatg atcctcaaac gcgtgcgcac gcccgagcag cccgatgggc
     4081 tggcggccca cggcttccgc cgggcgtggg cggcactcgt ctcgggcgag gatccggcca
     4141 ccctggcgct gcgcgagacg gccctcgccc tgagcgccgt gcaactgggg ggtatggact
     4201 tcgacgtcct gcgccgcgtc ggcctctccc ccgagcagat cctgggagtg ctgcgccgtg
     4261 gcttcgaggc cgcgacggcg tccgtggacg aggggctgcg cgatcgactg cgcaccgcgc
     4321 tcgccgaagc ctccacgtgg gtgggcgccg agcctcccgc cttcgtggag catctggtcc
     4381 gccagccgcg ggccggtccc ccccacgcca acacgcagcg ggtggcgctc ctgcccgcgg
     4441 agagccacgc cgaccactgc tacgtggtgg ccgtcgcctc ggtgctcgtc tcccccctct
     4501 tcggggcgga tcccaccggg cccttcgtgg ccgcgctgag ccatcacctc gccaacgccg
     4561 tgttgcccga cacgggcggc cacgggaacc agctgctcgg ggagcacctg gcgcccctca
     4621 tggcgcgcat caccgagcag gtgctcgaca cgctcccgtc cgagctcgcc caccgcgtgc
     4681 gcgaggcgcg gcggctcctc tcccatatcg acacgcccga ggcccgcgcc ttccatgccg
     4741 ccaacacgct ggatcgggtg ctggagacgg acgcgcacgc ccgcgccgcg ggcttcaccc
     4801 cgcgccaggc gctggaggac ctggagttca cccacgacga gcccatacgg gcctacgaga
     4861 acgcggtgct cgccgcggtg gggctctccc actgagcacg accctggagc cgtgccgacc
     4921 cgtgcccatg ccctggcccc gttcgaccgg ctgctgccgg gcatgccgca cacccggccc
     4981 cttgccctga aggagttgtc atgagcgcga agaagagtca gcaggaagcg tgtgaagcct
     5041 accgcgcggc ctttccgccc ggcgagctgg agatgttccg cgcggatccc atcgatcgcg
     5101 tgggcatccc ggcggtggcc gcgagcctgc ggctgaagac cggcacgcag ttcgccaccc
     5161 atggtcacgg caccaccccg gaggaggccg aggccagcgc gctcgggaag ctggccgaga
     5221 gcgtcttcac cgagacctcg ctccgggcac acgcgcgcga gaggggcacc taccgcgagc
     5281 tggcgcgcac ccgcggtccc tccggcgtgg tggatccgct gaagctgtgc ctccccgcgg
     5341 gcagccccta ccacgcggac atggagctga cgtgggtgac ggtgacgcgg ctgaccaccg
     5401 gggagaaggt gctcctcccc gaggaatggg tggccaccag gagcgggcag ctccagggcc
     5461 gcacgcccct catctccccc atcacccacg gccagggcgc gggcctcacc cacgagcagg
     5521 ccctgaccca cggcctcctc gagctgttcg agcgcgacgg caacggcctg cgcacccgcg
     5581 cgctcgatca aggcgtggtg atcgacctga aggacgcggc cctcggcccc gagctccagt
     5641 ccctgctcgc gcactgcgag cgcctgggca tcgagatcat tccgaagctg gcctgcacgg
     5701 acttcgggtt ggtgggcctg tacgtggtgg gacgcgaccc ggcgctgcgc gatcagccca
     5761 tggccctcac ggcgtgcggc gaggcggcgg acctggatcg ggagcgggcg ctgcacaggg
     5821 cgctgctgga gtacgcgggg tcgcgcacgc gcaaggcctt cgccaacggc cccctggagc
     5881 tggcggagaa gatcgcccct ccggggtaca tggaccgctt cctgccctac ctcgagctgg
     5941 aggacgagga gccccgcgcc ctgcaggcca tggccgactg ggcccagctc tccgcggccc
     6001 agatgcgctc gctcatggag cagcgggtgc tcgccgagcg aggccggatg cccttcgcct
     6061 cgctgcccac caccccgaac cccggtgacg cccggcagcg cgccgacgtg ctggtgcgca
     6121 agctcaccca ggcgggcttc gacatcctcg tggcggacat gtcgcccacg aaccactccg
     6181 tcgtctcgct caaggtcatc gtcccggggc tggaagcgga gacgatgagc gaccaccgca
     6241 tcggcgagcg cggcgtggcg aagctgctcg cgcggcagga tccactcgcg ggcctgggca
     6301 agccgcccga gggcgcccag cgcgtgcgcc tcacgccgga ggccgaggag cggctcggag
     6361 gccccacctg gttccactcg cgcctggcgg aggcgatcgt cggccggctc tacgcgctct
     6421 accgcgagcc cgagcgccac accgcccagc tcatgccgcg ccagcgccgc tacggaggcg
     6481 acccttcgtg agcccacggt tggacctgct cggcccgcct tcctcctgac gtgtgggaag
     6541 cacttggccc acgagccccg tgaatcctcg cggcgcctgg ggtaggacgc gccctcccat
     6601 gcaggaggag gacacggtgc tgctcaagga tggcgactgc gtcgccgaga tcgtcccgga
     6661 acgtggagcg ctcgtcagcc gcttcaccgt cggcggagaa gagctgctct tcctcgacgc
     6721 gagcaccctg gcggaccccg gcaagaacgt ccggggcggc atccccgtgc tcttcccctc
     6781 tccgggagtc cttcccggag gcacctaccc cgccgagggc cgcgactaca cgatgcgccg
     6841 ccacggcttc gcccgggatc tggcctggga ggtgcgccac cgggagaacg cgcgcacgga
     6901 gctcgtgctc gggcactcgg accagacgct gcgcgagttt ccctggcgct tcgaggcgcg
     6961 cctcaccgtg acgctctcgg gcggcgcgct gcgcctgggc ttcgccgccg agaaccgcga
     7021 cacccggccc atgcccctgc acctgggcta ccacccgtac ttccacgtgc cccaggcgaa
     7081 caaggccgtc acgcggctcg acacggacgc cacgcgcgcc tgggacaacc gcaccggagc
     7141 gcccgtcacc ttttccggcc tggacctcac cagcgccgag gtggacatgc acctgctcga
     7201 ccactcccgg ccgggcacca cgctccatcg cggtccgggg ctgcggccgg tggtgttgtc
     7261 ctggagcgag agcttcacca cggtggtggt gtggacgctc acgggccggg acttcgtctg
     7321 cgtggagccg tggacggcac ccggcggcgc gctccgcacc ggcgaggggc tgctgagcgt
     7381 ggctcccggg gccaccttct cctcggagtt cgagatccgc gccggaaggg aataagacgc
     7441 ggccatgtcc tccttcctct cccacctcga gtgctcgcgc tgccaccaga cccatgacgc
     7501 ggaccggctg cagaacctgt gcacgtgtgg cggaccgctg ctcgtgcgct atgacctgaa
     7561 ggccgccgcc cgcgcggtgc gccccacgaa tctcgccggc cgcgtgtcct ccctgtggcg
     7621 ctaccgcgag ttgctgcccc tgcgcgatga caagcacctc atcacgctcg gcgagggcat
     7681 gacgccgctg tttcccctgc cccggctggg cgcctccgtg gggctgcccg acctgtggtt
     7741 gaaggacgag gggctcaacc ccaccgcctc gttcaaggcg cggggtgccg ccaccggggt
     7801 gagtcgggcc aaggagctgg gggtcaccgc gctcgccatg cccaccaacg gcaacgcggg
     7861 cggcgcctgg gcgagctatg gcgcgcgcgc gggcatctcc gtcaccctgg tgatgcccac
     7921 ggacgcgccc gccatgagcg tgctggaggc cacggccgtg ggcgcccagg cctatatggt
     7981 gcgcgggcag atcaccgacg cgggcgccat cgtgggccgc tcggccaggg cgcacggctg
     8041 gttcgaggcc gccacgctca aggagcccta ccgcatcgag ggcaagaaga cgatgggcta
     8101 cgagatcgcc gagcagctcg gctggagcct gcccgacgtc atcctctacc ccaccggggg
     8161 cggcgtgggc atcatcggca tctacaaggc cctcttggag atgcgcgagc tgggctggtt
     8221 gccggacagc gtgcgccttc ccaagctcgt cgccgtgcag gccgagggct gccagcccat
     8281 cgtgaaggcc ttccgcgagg gcaaggacgt ctcggagaag tgggagaacg cgtccacggt
     8341 ggcgcagggc atccgcgtgc ccaaggcgct cggggacttc ctcgtgctcc aggcggtgcg
     8401 cgagacgggc ggcacctgcg tggccgtgtc cgacgcggac acgctgtggg ggctcgagca
     8461 gatcagccgc caggagggcg cgttcatctg ccccgagggc gccgcgctcg tgggcgccgc
     8521 gcgccagttg ctgcgcgagg ggtggttgga cgcgggccag cgcgtgctcc tgctcaacac
     8581 gggcgcgggc atcaaatacc cggacgtgat gacgcccaag ctccccatcc tggagctgaa
     8641 cgcgaccctg taatggtcag aaggcccggt tcaccgcgta ggcggcgatg ttccacccat
     8701 ggaaggcacc ggtgggcgcc tgccaaccac aaagtaggac attccgtcag gacagccgct
     8761 gacgcgaaga gcggcccgtg ccacgggaaa gttccccgcg tgggcatgtt tgtcttctcc
     8821 ccaagccggc tcgggaatca agcagccggg gattccccga agacttgagc ggcgaggtat
     8881 gcgagaacgg cgggctaccg cgaacgggac cgggaagcag aagacccggt cggtgcgcgg
     8941 ggcggcggag gtgttcgccc cgcggacagc ggtccctcgg gaggaagtcg agcatgcagc
     9001 ttcggacgat ggtgtggatg cgagtcgccc tgagcgcgct ggccctgggg ggaacggcct
     9061 gcgggtcgtt ggacctgtcg tgccagagag accaggactg tctcgactcc gaactctgcc
     9121 acccggacga gcaggagtgc gtgcgtcgct gctccacgaa cctggactgc tacgaggcgc
     9181 ggccgaagtg ccaggagatg ggctcctcga actccacgag gatctgcaag gcatgagcaa
     9241 ggacctggac gtccaccacc cggacaccgg aagcgccctc cggccccttg gaaggagaag
     9301 gagcatgaaa gcggtgtctt ccaccttttc tggacccacg tagaatggct gggaggacaa
     9361 cccaatgccg atgagcagca ccccgacggc tccgctcccc tggcgccccg atccattatc
     9421 ctggatggcc gcttcccctc ccgggttccc gggcgcgggg gtgcgtccac cgatcgcgtc
     9481 gagcgggccc agggcggagg ggcagggcca gcggcgcatc ctggtggtcg acgatgaccc
     9541 ggccatcctc aaggtctggc gccgggtgct ggggacccgc accgctccct cgtcgttgga
     9601 tcacctggag aagaagctct tcggggccac tccgcccgcc gcgcccacgg acgcggggtt
     9661 cgccatcgac accgcctcgc agggcatgga gggctaccag aaggtcgtca gtgccctgga
     9721 ggaggaccgg ccctacacgt tcgccctcgt cgacatgcgg atgccgcccg gctgggacgg
     9781 gctggagacc atcctgcaca tgctggagaa ggacccccgg ctggaggtgg ccatctgcag
     9841 tgccttctcc gacatctccc gggatgaagt cgcccagcgc gtgggccgct cggatctgct
     9901 gtggatccgc aagccgttcg agctcgaggc cgcgcgcgac ctcgcccgga agttgagcga
     9961 gcagggcgtg caccggcgcc agacgcgctg agcaggaact cagcgcttgg acggagcggg
    10021 cggtggggac ggcttgcgcc cgggggcggc ggcgggcttg gaggaagccg cggtgacgtt
    10081 cgtgcgcgcc ggcgtcttgg tctcgggctt cggggccggg ggaagtaccg cggggctcgc
    10141 cgccagcacc gccttcaccg actcgcgcag ctcgcctcgc accacctcgc gcaggtcctc
    10201 tcgcaacgcc gcccgcacct cgcgccggat gagctcctgg agctgctcgc cctggagcga
    10261 cgtcttcaac tcggcccgca ccagggacgt catgtcctcc tgcagctcgc cgcgcaggcc
    10321 cttcttgaag tccgtcttga gcgacgtgtc cagccccttc acgtccgcgt ggatggggtc
    10381 caacgccttg ttgatgccct cgagcgtggc gcccgtgacg gcgcggatga gctgctgggt
    10441 gctctcgctc tggctctgca gctccgtctt gaacgagccc agcgcgtcac gcatcacccc
    10501 gagcgaggcg atctcccgct tgagctcctc cagctcctgg gcacgcgcgt ccagctcgcc
    10561 cttcttctgc tcgagcttct cctgggtctc gcgcaacagc atcgtctgct gcgagcagcg
    10621 cgtgtccacg gacagcaggt tggcgtaggt gccgctcgtc acctggcaca tcgacagcgt
    10681 ctcctgctcg tcgagcagca cgagcgagac gctcagcgcc accaggacga cggtgagcat
    10741 ggacacggcg gccccccgcg tgatgctcag gctcaggccc tgacgctcga gccagccctt
    10801 gcgctgcacg cccagggtgc ccgccaggga gatgaccacc agcgacagca acgacagcat
    10861 cttgcccggg gagatctcca acaccagccg ggagagcagt tggagggact gccacgtcga
    10921 gcccttctcg atgtcgagct cggggcgggc gccaccctgc tgcgcggtgc cccaggtggg
    10981 ctgatgaacc acgcagcggc cgcgcatgtc gctcgcccac cggggctcgc tctgcgcgag
    11041 cggggtgctc ggacacaggc tgaagatgta gtccgtggtg ccggtgctga acgcgtggtg
    11101 catccgctgg cgatcagagg acttcgagag ggcccacctc cacgtgtagc cgagggcggc
    11161 gatcgccagg acgagcgcga cccagggcag ggcctgcttc acgaagtttc gcggggacat
    11221 cgggtggact cctgagggtg gcacgagagg atcgtccaag cgccgccagg aaacaacgca
    11281 ctgccccggg cgattgtccg tcgcacagcg gcccggagag cacccccacc ggtcacgcgg
    11341 aggccaccgc acccaggcga ggcgagggag cgagcacccg ggcgaggtag tcctccacgc
    11401 gctccgtgcc gaggcccggc gtgacgagcc cgaggtagcc atccgggcgg accagcacga
    11461 gcgcggggcc ctcgaggtca tacccccggc g
//
