LOCUS       NZ_CP022098            10843 bp    DNA     circular CON 17-FEB-2024
DEFINITION  Cystobacter fuscus strain DSM 52655 chromosome, complete genome.
ACCESSION   NZ_CP022098
VERSION     NZ_CP022098.1
KEYWORDS    RefSeq.
SOURCE      Cystobacter fuscus
  ORGANISM  Cystobacter fuscus
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Archangiaceae; Cystobacter.
REFERENCE   1  (bases 1 to 12349744)
  AUTHORS   Rupp,O., Goesmann,A. and Sogaard-Andersen,L.
  TITLE     Sequencing and comparative analysis of myxobacterial genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12349744)
  AUTHORS   Bruckskotten,M., Treuner-Lange,A., Rupp,O., Goesmann,A. and
            Sogaard-Andersen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUN-2017) Department of Ecophysiology, Max Planck
            Institute for Terrestrial Microbiology, Karl von Frisch Str. 10,
            Marburg, Hessen 35043, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2016
            Assembly Method                   :: Celera Assembler v. 8.1
            Expected Final Version            :: yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 10:04:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,093
            CDSs (total)                      :: 10,016
            Genes (coding)                    :: 9,906
            CDSs (with protein)               :: 9,906
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 26 of 110
            Pseudo Genes (incomplete)         :: 101 of 110
            Pseudo Genes (internal stop)      :: 1 of 110
            Pseudo Genes (multiple problems)  :: 17 of 110
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:24:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 10470810
            Orig. end                         :: 10481653
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10843
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="53"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10843
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10843
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5843](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5843)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            249..1187
                     /locus_tag="CYFUS_RS42435"
                     /old_locus_tag="CYFUS_008388"
     CDS             249..1187
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623777.1"
                     /locus_tag="CYFUS_RS42435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008388"
                     /product="oxygenase MpaB family protein"
                     /protein_id="WP_095990398.1"
                     /transl_table=11
                     /translation="MALRDPVPAEREAAPEQTAKRAAAREWKIDYLSPAGEPSLVPPDS
                     VQWRVYRNPIVMGIGGVAAVLLEFADPRIRSGVWDHSVYKVDPLGRSRRTGIAAMVGVY
                     GPASVARKVISGVTKMHARVSGETPNGQSYRALDPVLLNWVSATAAFGFFKAYHTFVRP
                     LSPEDQARFFREGKPVAELYGVTHSVGSEAEFVAMMEELLPGFEPHPINLEFLNIIESG
                     RAAPSVPRFLHRAMARGAVAILPPVVRKKLELGAEWDLTALDRLALTSAGRLADHWRDR
                     DSPAWQAALRHGLPGDFAWRSPSVQRSLLKN"
     misc_feature    255..257
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1353..1718
                     /locus_tag="CYFUS_RS42440"
                     /old_locus_tag="CYFUS_008389"
     CDS             1353..1718
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623778.1"
                     /locus_tag="CYFUS_RS42440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008389"
                     /product="hypothetical protein"
                     /protein_id="WP_232537126.1"
                     /transl_table=11
                     /translation="MLLAGAGHLTWARTEFLAQVPKWVPLDADFVVLASGVVELVLGAA
                     LIFARRLRTQVGWAAAAFFVAIFPGNISQYVNGISAFGLDTDRARFIRLFFQPVLVACA
                     LWSTGAWAAYRASKRSK"
     gene            complement(1735..2280)
                     /locus_tag="CYFUS_RS42445"
                     /old_locus_tag="CYFUS_008390"
     CDS             complement(1735..2280)
                     /GO_process="GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF07366"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623049.1"
                     /locus_tag="CYFUS_RS42445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008390"
                     /product="ester cyclase"
                     /protein_id="WP_095990399.1"
                     /sec_met_domain="PF07366 (E-value: 1.3e-14, bitscore: 53.3,
                     seeds: 29, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTEQVREARQKLVLNHFHDEVKQNWDDVLSTFPHPRYELIPTLT
                     VHDGDSRVRRYYRETRIAFPDQSHEIIALRHSDDAVIVEFWLMGTHLGPLGQIPPTGNR
                     FRVRVTAYFIFDATETLVCERVYFDTLSMLKQLVGGLDMKQPKNWLLAARCFKGLLAMS
                     NDEPVPSLTETTPPIFNA"
     gene            complement(2325..3428)
                     /locus_tag="CYFUS_RS42450"
                     /old_locus_tag="CYFUS_008391"
     CDS             complement(2325..3428)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623048.1"
                     /locus_tag="CYFUS_RS42450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008391"
                     /product="hypothetical protein"
                     /protein_id="WP_095990400.1"
                     /transl_table=11
                     /translation="MRREWLDSNVADMSGIGARIDAFDGMERVPGGSGARLRWARTRAA
                     RFRSEFVSTGTPDSVDTCDLVTMPYPTAFGLFRASTALSPFLSITNRMLVIRWRESDGR
                     RRVLLFEPSDFELGRKTPYFAALGARVPKPLHSLLVKEHGTVLDHLARMRIAPEEVDYL
                     MFDHLHTQDLRRWVGTTAPQEDLGGVVKPLLPNARVIVQRDELAAMADLHPLQQPWYQA
                     ATFADLRPDVFLAIEGGHVLGPGVAVVATPGHVLGNQSLVLNTSTGIWASSENAIAAEC
                     LTPEHSRMPGLARWARTWGQEVILNANTLETTVDQYNSLILEKTLVDRSQADERFLQFF
                     PSSELTAAWTHPGTAPTFSHRAIHHRG"
     gene            3498..4250
                     /locus_tag="CYFUS_RS42455"
                     /old_locus_tag="CYFUS_008392"
     CDS             3498..4250
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1239: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623047.1"
                     /locus_tag="CYFUS_RS42455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008392"
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="WP_232537127.1"
                     /transl_table=11
                     /translation="MKRSDRREPDMEPAGEAPRRGRPRGGPRLTREKVLEAALTLVDRG
                     GIETLSMRSLATELGVDAMSLYNHVANKDAVLDGIAELFLGRIGLPEPTGDWRADVRAL
                     AAAFRHAAAQHPQAAPLVLTRQLESFKGLVGTESALGLLHAAGFPPEEAVHALRTVLAF
                     LVGTLLREVSSGPTFSGQNLGGLVERRVELENSGFPHVARAALHLAACNHEAEFDFGMD
                     LLVAALEQRARARRPTATTADRRTRKPR"
     gene            complement(4288..5001)
                     /locus_tag="CYFUS_RS42460"
                     /old_locus_tag="CYFUS_008393"
     CDS             complement(4288..5001)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623046.1"
                     /locus_tag="CYFUS_RS42460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008393"
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="WP_198316331.1"
                     /transl_table=11
                     /translation="MPGLQHFFESMRTFLAEPGATGLERLYGAHPGWEAPRARVALYGR
                     FVRHHVADGVEKLFPLVRRCVGEVAWEELVRGYFSTGPARHYELNRLGEHFPDFLSDKA
                     PERGLPAWVPELARFEWTDFAVYASEERVPVTVERLSPNPTLVVLRHSCRLCPFVRGGA
                     RGEPRSGEELALLWRHPRDLVSLYMAADDVALLALKMAVEALAPAEVAVATGVAESDIR
                     AAVERCVADGLVLTP"
     gene            complement(5001..5843)
                     /locus_tag="CYFUS_RS42465"
                     /old_locus_tag="CYFUS_008394"
     CDS             complement(5001..5843)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637556.1"
                     /locus_tag="CYFUS_RS42465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008394"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_095990402.1"
                     /sec_met_domain="DUF692 (E-value: 3.4e-94, bitscore: 314.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGRSWRQGLKPLGAGIGLRREFHEQLPRTARVLDWVEFVSENFLT
                     LGGRAQRALDACAERWPVIPHGVGLDIGGPEPLNEDYVTRLAALVARVDSPFFSDHLCY
                     ARLGGVYLHDLLPLPFSEDAVEHVVPRIREVVARVERPFLLENITYYAHMPGRTLSEAA
                     FLRAVVEEADCGLLLDVNNVYVNARNHDYDPRAFLDALPLERVVQVHLAGHTELPDMLI
                     DSHGDTVRDEVWSLYQYLLERTGPVSTLIEWDQDIPSLDAVLDEAARARAVLGALGER"
     gene            complement(5847..6146)
                     /locus_tag="CYFUS_RS42470"
                     /old_locus_tag="CYFUS_008395"
     CDS             complement(5847..6146)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350980.1"
                     /locus_tag="CYFUS_RS42470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008395"
                     /product="hypothetical protein"
                     /protein_id="WP_095990403.1"
                     /transl_table=11
                     /translation="MNVKTLAAIVGTLSLGSLATGCATTHRSAEPGTENGTQGAGSTRG
                     GEASCGENTCSGKATQKGGEASCGEGTCGGGQKAATPEKGSHASCGESGCGGGR"
     gene            complement(6225..6734)
                     /locus_tag="CYFUS_RS42475"
                     /old_locus_tag="CYFUS_008396"
     CDS             complement(6225..6734)
                     /GO_function="GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /locus_tag="CYFUS_RS42475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008396"
                     /product="RICIN domain-containing protein"
                     /protein_id="WP_157758972.1"
                     /transl_table=11
                     /translation="MMSRMLSVAVVAAMTTLGLSTSARAESTPLVITLKDSPQVMTLGG
                     GPEGQEGAAVMLTPNKTQPGQGFSLKPVGKPEDKTFNIVSQANQLCVDVENGSREDGAA
                     IIQKPCNGASCQVFRVSDRGADGHRELRNELSGKCLAASHGKSSGLIQAACTGQDNQRF
                     RISPSR"
     misc_feature    complement(6465..6467)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6806..7384)
                     /locus_tag="CYFUS_RS51340"
                     /old_locus_tag="CYFUS_008397"
     CDS             complement(6806..7384)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CYFUS_RS51340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CYFUS_008397"
                     /product="hypothetical protein"
                     /protein_id="WP_157758973.1"
                     /transl_table=11
                     /translation="MPTEFIEKDERGRVRERYFEDDEGRLDGVAATYDEDGRLVQESHW
                     RAGLLHGPTSLYDSDGQVVQRVMFADGQLHGPAELQDEQGRFRMQLGFHEGRTHGELLA
                     WRDGQPLMKHTLEQDRPEGPLELYEEGKLTRRVLFERGWPLVPGQPAEQDSMAPGPEAG
                     TDRPAEQERQAREQPSGWLQGWLRRGGGS"
     gene            complement(7401..9251)
                     /gene="tssI"
                     /locus_tag="CYFUS_RS42480"
                     /old_locus_tag="CYFUS_008398"
     CDS             complement(7401..9251)
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /gene="tssI"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /locus_tag="CYFUS_RS42480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008398"
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="WP_095990405.1"
                     /transl_table=11
                     /translation="MRSGYTQDNVYLSVTTPLGKDVLLLHGFQGEEALSRPFHFTLELH
                     SERLDVDFSRVVGKGAAISLAQRTGGERFFHGIITRFVQAGTFGDFTRYLAELRPWFWL
                     LTLTRDSRIFQNLTVPQILQQLFQEQGFTDFRLALRRTYTPREYCVQHQESAFDFASRL
                     MEDEGIFYFFEHTKDRHTLVLADDVAAHEPCPGPGTAKVQGHLDEARTEDAITGCELEQ
                     QVVPGRYALGDYFFETPSTRLQAQVKGKEGRQEQYEYPGAFTRRDVGEQRGRIRLEAHE
                     AQARTLRGQGHVRWFIPGYRFTLAEHERQDINGAYVLCWVSHSATVGAYSNSFEAIPAA
                     TPFRPPRVTPRPVVHGVQSARVVGKAGEEIWTDRFGRVKVQFHWDREGQKDERSSCWMR
                     VAQGQAGKGWGHFFLPHVGQEVLVTFLDGDPDRPIVTGSVYNAEQVVPYPLPAGQTRST
                     VRGDASGGGQPNELRFEDKKGAEELYLHARRDMNVSVERDSVTEIQGNCTIRVRGNLTL
                     DVRGSIDVKAGRNLTQEAKMSLKSIAGMNLTQNAKLALESSAGTMLRHSSKLMMMADAQ
                     TLISNVKLMHMLKATPLMILGPMIPLPMGGPPLPPIPPTP"
     gene            complement(9248..10276)
                     /gene="tssG"
                     /locus_tag="CYFUS_RS42485"
                     /old_locus_tag="CYFUS_008399"
     CDS             complement(9248..10276)
                     /GO_process="GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /gene="tssG"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /locus_tag="CYFUS_RS42485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_008399"
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="WP_095990406.1"
                     /transl_table=11
                     /translation="MEEMAPHGRRRAPGVAQELFESGHRFDFFQAMRLLRLLRPKEPVR
                     LRASVELAFPASDIVQVMPPARPGGTPEMTVAFLGLGGVQGPLPRPFAQQLRDRTRVGD
                     TGLRDFLDIFHHRLLSLLYQGRVRRRVWLEPGVPEAHDVARYLYALMGLGTRGTRGRLE
                     VEDRRLLRYAGLLAHRPVSLEALRAMLSDALGVGVKPRAPRGAWMELEEEQRTRLGPTG
                     RNQRLGQGAVLGGRAWLEQAGLELELGPLSWRRYVELLPGGGGLSTLRSLTRFALGPGP
                     EVWLVLVVRSGDIPEQPLGAPSGPRLGWTSWLRARPGRQGTQAVALSPRHMSAHGARED
                     GT"
ORIGIN
        1 aacagcgcat gcccgcctcg aggatccggg ccctggtcat ctccgtcggc gtcaggcggc
       61 ttttggtctc gaggctcatg gtcagccata taggccggga ggagcccgtt ccggtagggc
      121 acactcctga caggttcctt ctcgggcgag catgcggaac gtcctcacga ccagaacagg
      181 cactccggtt gttgcggcgg atcctatcac ctgatatacg gaatatatca tctgatagag
      241 gtgacgagat ggctttacgt gatccggtac cggcggagcg cgaagcagcg ccagagcaga
      301 cggcgaagcg ggcggccgcg cgcgagtgga agatcgacta tctctccccc gctggagagc
      361 cttcgttggt cccgccggac tcggtgcagt ggcgggtcta ccgcaacccg atcgtcatgg
      421 gcatcggcgg agtggcggcc gttctgctcg agttcgccga tccgcggatc cgcagcgggg
      481 tctgggacca ttcggtctac aaggtggacc cgctcggacg ctcacggcgt accgggatcg
      541 cggcgatggt gggggtgtat ggccccgcct cggtggcgcg caaagtgatc tccggcgtca
      601 cgaagatgca cgcgcgcgta tcgggcgaga cgcccaacgg gcagtcgtac cgtgcgctcg
      661 acccggtgct gctcaattgg gtctccgcga cggctgcctt cggcttcttc aaggcctacc
      721 acacgttcgt tcgtccgctc tctccggagg atcaggcgcg ttttttcagg gagggaaagc
      781 cggtcgccga actctacggc gtcacgcaca gcgttgggtc ggaagccgag ttcgtcgcga
      841 tgatggaaga gctgctgcca ggtttcgagc cgcacccgat caacctcgag ttcctgaaca
      901 tcatcgaatc gggccgtgcg gcgccttccg tgccgcgctt cttgcaccgc gcgatggctc
      961 gcggcgcggt cgcgatcctg ccgccggtgg tccgaaagaa actggagctc ggtgcggaat
     1021 gggatctgac cgcgctcgac cgcctggcgc tgacctccgc cggccggctc gctgaccact
     1081 ggcgcgaccg cgactctcca gcctggcagg cggcgctgcg tcacgggctt ccaggcgact
     1141 tcgcctggcg atcgccgtcg gtgcagagaa gcctgctcaa gaactgacgc tcacacatcc
     1201 gatgtgcgct cgaggcggtc acccccgatg cgaggtgacc tccacgaact tcccatggag
     1261 gactcatgtc tcaagtcatg actcaacaag acgttgccgt ggcacgcccc agtctcctga
     1321 ggacccttgc acgcatctgg ctgggagcct ccttgctcct cgcgggggcg ggccacctca
     1381 cgtgggcccg caccgagttc ctggcccagg tcccgaagtg ggtcccactc gacgccgact
     1441 tcgtcgtcct cgcttcgggc gtggtcgagc tcgtgctcgg cgcggcgctc atcttcgcgc
     1501 gccgcctgcg cacgcaagtc gggtgggcgg cggccgcgtt cttcgtcgcg atcttcccgg
     1561 gcaacatctc gcagtacgtc aatggcatca gcgcgttcgg tctcgacacc gacagggctc
     1621 ggttcatccg gctcttcttc cagcccgtcc tcgtcgcctg tgccctctgg tcgaccggcg
     1681 cgtgggcggc ctatcgtgcg tcgaagcgct cgaagtgacg gcttctcctc cgccctatgc
     1741 gttgaaaatg ggcggggtgg tctcggtcag ggaggggacc ggctcgtcgt tggacatcgc
     1801 caggaggccc ttgaagcacc gggcggcgag gagccagttc ttcggctgct tcatgtcgag
     1861 gccgcccacg agctgcttga gcatgctgag ggtgtcgaag tagacgcgct cgcaaaccag
     1921 cgtctcggtc gcgtcgaaga tgaagtacgc cgtcacgcgc acccggaacc tgttcccggt
     1981 agggggaatc tggcccagcg gacccaggtg ggtgcccatc aaccagaatt cgacgatgac
     2041 ggcgtcgtcg ctgtgccgca gcgcgatgat ctcgtggctc tgatccggga acgcgatgcg
     2101 ggtctcgcgg tagtacctgc gcaccctgct gtcgccgtcg tgcaccgtca gggtggggat
     2161 cagctcgtaa cggggatggg ggaaggtcga caggacgtca tcccagttct gcttcacctc
     2221 gtcgtggaag tggttcaaca ccagcttctg ccgtgcctcg cggacctgct cagtgggcat
     2281 gcgtgcctct tttcttgggg gaaggacttc aggggaaggg gcggtcagcc ccggtgatgg
     2341 atggcccggt ggctgaacgt gggggccgtg ccagggtggg tccatgcagc ggtgagttcg
     2401 ctggacggga agaactggag gaatcgttcg tccgcctggg accggtcgac cagcgtcttc
     2461 tcgaggatca gcgagttgta ctggtccacg gtggtctcca gggtattggc gttgaggatg
     2521 acctcctgcc cccaggtgcg cgcccaccgc gcgagccctg gcatgcgcga gtgctccggg
     2581 gtcaggcact ccgcggcgat ggcattctca ctgctggccc agatgccagt ggacgtgttc
     2641 aggacgaggg actggttgcc gaggacgtga cccggcgtgg cgaccacggc gacaccgggc
     2701 ccgaggacgt gccctccctc gatggcgagg aagacgtcgg gccggaggtc cgcgaacgtc
     2761 gccgcctggt accacggctg ctgcagggga tgcagatccg ccatggcggc gagttcatcg
     2821 cgttgaacga tgacccgggc attgggaagg agaggcttca cgacgccgcc caggtcctcc
     2881 tggggggcgg tcgtccccac ccagcgcctc aggtcctggg tgtgcaggtg gtcgaacatc
     2941 aggtagtcga cctcctctgg agcgattcgc atgcgcgcca gatggtccag caccgtgccg
     3001 tgttccttca cgaggaggga atgcaggggc ttggggacac gcgcccccag cgccgcgaag
     3061 tacggcgtct tgcggccgag ttcgaagtcg ctcggctcga agagcagcac gcgccgccgg
     3121 ccatcggact cgcgccagcg gatgaccagc atgcgattgg tgatggacag gaagggagac
     3181 agcgccgtgc tggcgcggaa cagtccgaac gccgtggggt acggcatcgt gaccaggtca
     3241 caggtgtcca cgctgtcagg cgttcccgtc gacacgaact ccgagcggaa ccgggcggcc
     3301 cgcgtccggg cccaacgcag cctggcgccg gagcctcccg gcacgcgctc catgccgtcg
     3361 aaggcgtcga tgcgcgcgcc aatcccggac atgtccgcga cattggaatc cagccactca
     3421 cgccgcatcg agcaccttcc gcgtttacct acgctgtaga ctggaagtaa tcctacgacg
     3481 taggtattgt caacgtcatg aagcgttccg acaggcgaga gcccgacatg gagccggccg
     3541 gcgaggcacc ccggcgcggc cggccgaggg gaggtccccg gctcacgcgg gagaaggtcc
     3601 tcgaggccgc cttgacgctc gtggaccgtg gagggatcga aacgctgagc atgcggagcc
     3661 tcgccacgga gctcggcgtg gacgcgatga gcctctacaa ccatgtcgcc aacaaggatg
     3721 cggtcctcga cggaatcgcg gagctgttcc tgggacgcat cggcctgccc gaaccgaccg
     3781 gtgactggcg cgcggatgtc cgcgcgctcg cggcggcctt ccgacacgcg gcggcccaac
     3841 acccccaggc cgccccgctg gtgctcacgc gccagctcga gtccttcaag ggcctcgtgg
     3901 gcacggagtc agcgctcgga ctgctgcatg cggctggttt tccaccggaa gaggccgtcc
     3961 atgccctgag gaccgtcctc gccttcctgg tcgggacgct cctgcgcgag gtgtcctccg
     4021 ggccgacctt cagcggccag aacctcgggg gcctggtgga gcgccgcgtg gaactcgaga
     4081 actccggctt cccccacgtg gccagggccg cgctccacct ggccgcgtgc aaccacgagg
     4141 cggagttcga cttcggcatg gacttgctgg tggccgcgct cgagcagcgg gcacgcgccc
     4201 ggcgacccac cgcgacgacc gctgaccgcc gaacgcgaaa accacggtag ggccactcaa
     4261 aaaaacaagc ctggagataa cgtctgctca tggggtgaga accagcccat ccgccacaca
     4321 gcgctccact gccgcgcgga tgtcggattc cgccacgccc gtggccacgg ccacctcggc
     4381 cggagccagc gcctccaccg ccatcttcag ggccagcaac gccacgtcat ccgcggccat
     4441 atacaggctc accaggtctc gcgggtggcg ccacagcagc gccagttcct cacccgaccg
     4501 cggctcgcct cgagcgccac ctcggacgaa gggacatagc cgacaggagt gccgcaacac
     4561 cacgagcgtg ggattgggcg agagccgctc caccgtcact ggcacccgct cctccgaggc
     4621 gtacaccgcg aagtccgtcc actcgaagcg cgccagctcc ggcacccacg cgggcagccc
     4681 acgctccggc gccttgtccg agaggaagtc cgggaagtgc tctcccagcc gattgagctc
     4741 gtagtgccgg gcgggcccgg tggagaagta gccccgcacc agttcttccc aggcgacctc
     4801 gcccacgcat cgcctcacca gtggaaagag tttctccact ccatccgcca cgtggtggcg
     4861 cacgaagcgc ccatacagcg ccacccgcgc gcgcggtgcc tcccacccgg ggtgcgcccc
     4921 atacagccgc tccagtcctg tcgcaccggg ctccgccagg aaggtcctca tggactcgaa
     4981 gaaatgctgg aggcccggca tcaccgttct cccagcgctc cgagcaccgc acgcgcccga
     5041 gccgcctcgt ccagcacggc atccagcgac gggatgtcct ggtcccactc gatgagcgtc
     5101 gacaccgggc cggtgcgctc caggaggtac tggtagagcg accacacctc gtcgcggacg
     5161 gtgtctccgt ggctgtcgat gagcatgtct ggcaattccg tgtggccagc cagatggacc
     5221 tgcaccaccc gctccagggg cagtgcgtcc aggaaggccc tcgggtcgta gtcgtggttg
     5281 cgcgcgttca cgtacacgtt gttcacatcg agcagcaggc cacagtcggc ctcctcgacc
     5341 actgcccgga ggaaggcggc ctcggacagg gtgcgcccgg gcatgtgtgc gtagtaggtg
     5401 atgttctcca gcaggaaggg tcgctccacc ctcgccacca cctcgcggat tcgtggcacc
     5461 acgtgctcca ccgcgtcctc ggagaagggc agtggcaaca ggtcgtgcag gtacaccccg
     5521 cccagccgcg cgtagcacag gtggtcggag aagaaggggg agtccacccg cgccaccagc
     5581 gccgcgagcc gggtcacata atcctcgttc aggggctcgg gcccaccgat gtcgagtccc
     5641 accccgtggg gaatcaccgg ccagcgctcg gcgcaggcgt ccagcgcccg ttgcgcgcgg
     5701 ccccccaggg tgaggaagtt ctcggagacg aactccaccc agtccagtac tcgcgcggtg
     5761 cgagggagtt gctcatggaa ttcccgccgc agcccgatac cggcccccag cggcttcaat
     5821 ccctgacgcc atgaacggcc catgagctac ctgccaccac cgcaaccgct ctcgccgcag
     5881 ctcgcgtggg agcccttctc cggcgtcgcg gccttctggc cgccgccaca ggtgccctcg
     5941 ccgcacgagg cctcgccccc cttctgggtg gccttgccgc tacaggtgtt ctcaccgcac
     6001 gaggcctcgc cgccccgggt gctcccagcg ccctgggtgc cgttctccgt gccgggctcc
     6061 gccgagcggt gggtggttgc acatcccgtc gcgagcgagc cgagggacag ggttccgacg
     6121 attgctgcca gggtcttgac gttcatggag tgttttcctt gtcatgtgaa ttcttgcttt
     6181 gcgtcatgtt catcttccgg ccgtgagctg catggcgagg gctgctaacg cgaggggctg
     6241 atgcggaagc gttggttgtc ctggccagtg cacgcggcct ggatgagccc ggaactcttg
     6301 ccgtgtgagg ccgccaggca tttgccgcta agctcgttgc gaagctcccg gtgcccatcg
     6361 gcgcccctgt cgctcacgcg gaacacctgg cagctcgcgc cattgcacgg cttctggatg
     6421 atcgcggcgc cgtcctcacg gcttccgttc tcgacgtcca cgcataactg gttcgcctgg
     6481 gagacgatgt tgaacgtctt gtcctcgggc ttcccgaccg gcttgagcga gaagccctgg
     6541 ccgggctggg tcttgttggg ggtcagcatg acggccgctc cctcctgtcc ctccgggcca
     6601 ccgccaaggg tcatcacctg ggggctgtcc ttcagggtga tgacgagggg agtggactcg
     6661 gctcgtgccg aggtgctgag tcccagggtc gtcatggcgg caaccaccgc cacggacagc
     6721 atccggctca tcatgtgtct tcctttggtg tgaagggttg aggcgctcca cggattggag
     6781 tgccctctcc gctcatgtga tggagtcagg aaccaccgcc tcttcggagc cagccctgga
     6841 gccatccaga gggttgctcc cgtgcttgtc gttcctgctc cgcgggcctg tccgtgccgg
     6901 cctccggtcc aggtgccatc gagtcctgct ccgcgggctg ccccggcacc aggggccatc
     6961 cccgctcgaa gagcacccgg cgggtgagct tgccttcctc gtagagctcg agcggaccct
     7021 cgggtctgtc ctgctcgagg gtgtgcttca tcagcggctg gccgtcccgc caggcgagca
     7081 gctcgccgtg cgtcctgccc tcatggaatc cgagctgcat gcggaagcga ccctgctcgt
     7141 cctggagttc cgccggcccg tggagctggc cgtccgcgaa catgacccgc tgcaccacct
     7201 ggccgtcgga gtcgtagagg ctcgtgggcc cgtgcagcag gcccgcgcgc cagtgcgatt
     7261 cctggacgag ccgaccatcc tcgtcatagg tggccgccac tccatccagc cgaccctcgt
     7321 cgtcctcgaa gtagcgctcg cgcacccgcc cccgctcgtc cttctcgatg aattccgttg
     7381 gcacgggctc atccctccgc tcagggggtg ggcggaatcg gtgggagcgg aggccctccc
     7441 atcggaagcg gaatcatcgg gcccaggatc atcaggggcg tcgccttgag catgtgcatg
     7501 agcttgacgt tgctgatcaa cgtctgggca tcggccatca tcatgagctt ggagctgtgc
     7561 cgcagcatcg tgcccgccga gctctccagg gccagcttcg cattctgggt gaggttcatc
     7621 cccgcgatgc tcttgagcga catcttcgct tcctgcgtga ggttccttcc cgccttgacg
     7681 tcgatggagc cccgcacgtc cagggtgagg ttgccccgga cccggatggt gcagttgccc
     7741 tggatctccg tcacgctgtc gcgctccacg gacacgttca tgtcccggcg ggcgtgcaga
     7801 tagagctcct ccgcgccctt cttgtcctcg aaacgcaact cattgggctg tcccccgccg
     7861 gaggcatccc cgcgtaccgt gctccgggtc tgccccgcgg gcaacggata gggcaccacc
     7921 tgctcggcgt tgtagacgga gccggtgacg atgggccgat ccggatcccc atccaggaag
     7981 gtgaccagca cctcctggcc cacgtgtggc aggaagaagt ggccccagcc cttgcccgcc
     8041 tggccctgcg ccaccctcat ccaacacgag ctgcgctcgt ccttctggcc ctcccgatcc
     8101 cagtggaact ggaccttcac gcggccgaat cggtccgtcc agatctcctc gcccgccttg
     8161 cccaccaccc gggcgctctg cacgccgtgg acgactggcc gcggcgtcac ccggggcgga
     8221 cggaagggcg tggcggcggg gatggcctcg aagctgttgc tataggcccc caccgtggct
     8281 gagtgcgaca cccagcacag cacgtacgcg ccattgatgt cctggcgctc gtgctcggcg
     8341 agggtgaagc ggtagccagg gatgaaccag cgcacgtgac cctggcctcg cagcgtccgg
     8401 gcctgagcct cgtgcgcctc caggcggatc cggccgcgct gctcacccac gtcccggcgg
     8461 gtgaaggcac cggggtattc gtactgctcc tggcgcccct ccttgccctt cacctgcgct
     8521 tgcagccggg tggatggcgt ctcgaagaag tagtccccca gggcgtagcg tccgggtacc
     8581 acctgctgct ccagctcgca cccggtgatg gcgtcctccg tccgcgcctc atccaggtgc
     8641 ccctggacct tcgccgtccc gggacccggg cagggctcat gggccgccac atcgtccgcc
     8701 agcaccagcg tgtgccggtc cttcgtgtgc tcgaagaagt agaagatgcc ttcgtcctcc
     8761 atcagccgtg aggcgaaatc gaaggcggac tcctggtgct gcacgcagta ctcgcgaggc
     8821 gtgtacgtgc gccgcaacgc cagccggaag tcggtgaagc cttgctcctg gaagagctgc
     8881 tggaggatct gcggcaccgt caggttctgg aagatgcggc tgtcgcgggt gagggtgagc
     8941 agccagaacc aggggcgcag ctcggcgagg taccgggtga agtccccgaa agtgccggcc
     9001 tggacgaagc gggtgatgat gccgtggaag aaccgctcgc cgcccgtgcg ttgcgccagg
     9061 gagatggcgg cgcccttgcc caccacccgg gagaagtcca cgtcgagtcg ctcggagtgc
     9121 agctccagcg tgaagtggaa gggccgggag agggcctcct cgccctggaa gccgtgcagc
     9181 agcagcacgt ccttgcccag gggcgtggtg acggagaggt agacgttgtc ctgcgtgtat
     9241 cccgacctca tgttccatcc tcccgcgcgc cgtgcgcgga catgtgacgg ggcgaaagcg
     9301 ccaccgcctg ggttccctgc cggccgggcc ttgccctcag ccaggacgtc caccccaggc
     9361 gcgggccgct cggcgcgccc aggggttgct cggggatgtc gccggagcgc accaccagca
     9421 ccagccacac ctcgggcccc gggcccaacg cgaagcgggt gagcgagcgc aaggtgctca
     9481 gtcccccacc accgggcagc agctccacat agcggcgcca ggagagaggg cccagctcca
     9541 gttccaggcc cgcctgctcc agccaggcgc gcccgccgag caccgcgccc tggccgagcc
     9601 gctggttgcg tccggtgggg cccaggcgcg tgcgctgttc ctcttccagc tccatccagg
     9661 cgccccgggg cgctcgcggc ttcactccca cgcccagcgc gtccgagagc atcgcccgca
     9721 gggcctcgag ggacacgggc cggtgcgcca gcaatcccgc gtagcgcagc agccgccgat
     9781 cctccacctc cagccggccg cgcgtgcccc gggtgcccag gcccatgagc gcatacagat
     9841 agcgggccac gtcatgggcc tcgggcacgc ccggctccag ccacacccgg cgccgcaccc
     9901 gcccctggta cagcagcgac agcagccggt ggtggaagat gtccaggaag tcgcgcaggc
     9961 ccgtgtcccc cacgcgcgtc ctgtcccgga gctgctgggc gaagggcctc ggcagcggcc
    10021 cctgcacccc gcccagaccg aggaaggcca ccgtcatctc cggcgtgccc ccgggcctcg
    10081 cgggcggcat cacctggacg atgtcgctgg cgggaaaggc cagctccacc gaggcgcgca
    10141 gccgcaccgg ctccttcggg cggagcaggc gcagcaggcg catcgcctgg aagaagtcga
    10201 agcggtggcc ggactcgaag agctcctggg ctacaccagg ggcgcgtcgc cggccatggg
    10261 gggccatctc ttccatgtgt cctcccgctg ctcgctcctc gccacgagct gggtgaagga
    10321 atccaccggg gcgtacaggc cgaagaagtg gtggagcacc gccgcgagca gcagcgcact
    10381 gccgcccacg tagagggact cgtcgaaggt gagggtgagt tcctggccgc ggcagaagcc
    10441 ccgccaggca tcccagccgc gctggcgcac caccgggcgg cacgtcagcc ccgtcagccc
    10501 ctggagctgc tgctcgcaca cctggccgcc ggagaagtca tgcagccgca gcagctcctc
    10561 gagcgcctcc agcgcctggg ggccgttggc gagcgagagg tggttgagcg acagcaacga
    10621 gacgagctgc cagagcatgg cgccccgctc cgggggcgcg cggggtggcg tgggacggcg
    10681 caggcagcgg atggcgccca cggggacgcg ctcctccacc tggagctccg cgccgggccc
    10741 gagttgcacg ggcaactccc cattggtgca cagcgtgtgc acgtgcagcg tctgctccgc
    10801 cggcaggtgc gggtcgagct ccaggtccac gaaggacagc agc
//
