LOCUS       NZ_CP022098            10846 bp    DNA     circular CON 17-FEB-2024
DEFINITION  Cystobacter fuscus strain DSM 52655 chromosome, complete genome.
ACCESSION   NZ_CP022098
VERSION     NZ_CP022098.1
KEYWORDS    RefSeq.
SOURCE      Cystobacter fuscus
  ORGANISM  Cystobacter fuscus
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Archangiaceae; Cystobacter.
REFERENCE   1  (bases 1 to 12349744)
  AUTHORS   Rupp,O., Goesmann,A. and Sogaard-Andersen,L.
  TITLE     Sequencing and comparative analysis of myxobacterial genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12349744)
  AUTHORS   Bruckskotten,M., Treuner-Lange,A., Rupp,O., Goesmann,A. and
            Sogaard-Andersen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUN-2017) Department of Ecophysiology, Max Planck
            Institute for Terrestrial Microbiology, Karl von Frisch Str. 10,
            Marburg, Hessen 35043, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2016
            Assembly Method                   :: Celera Assembler v. 8.1
            Expected Final Version            :: yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 10:04:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,093
            CDSs (total)                      :: 10,016
            Genes (coding)                    :: 9,906
            CDSs (with protein)               :: 9,906
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 26 of 110
            Pseudo Genes (incomplete)         :: 101 of 110
            Pseudo Genes (internal stop)      :: 1 of 110
            Pseudo Genes (multiple problems)  :: 17 of 110
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:24:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8400474
            Orig. end                         :: 8411320
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10846
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="44"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10846
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10846
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5846](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5846)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(304..2397)
                     /locus_tag="CYFUS_RS33740"
                     /old_locus_tag="CYFUS_006673"
     CDS             complement(304..2397)
                     /EC_number="3.4.24.-"
                     /GO_function="GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624022.1"
                     /locus_tag="CYFUS_RS33740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_006673"
                     /product="M13 family metallopeptidase"
                     /protein_id="WP_095988966.1"
                     /transl_table=11
                     /translation="MKKALLAACCLVASACKTAEPAPTPPSPATPATQPQPPAESRPLS
                     PGLDLASMNEQANPCEDFYEFACGNWVKNTEIPADRPRWSRGFDAISARNEEILREILE
                     GASKGKAPEGLASAQKLGDFYGSCMDEAKLEASLPVLKAELAKLTVKNAKDLARVVGTL
                     HAQNVFPLFRMGANTDFKDASQMIGEVDQGGLGLPDRDYYLKDDEKSKALRDAYTEYVK
                     NTFVLLGEPAETAAKSAASVLEVETALAKASMSRVERREPKSLYHRLERKGLKAEAPGF
                     PWDEYFKAAGAGSVQALNVSHPPFFKEVERLAKTMPAAAWAPYLTWNYVSSVVPALPKA
                     FQDERFRFSQNLTGAKEDVVRWKKCVRFTNMALGEALAQPFIERTFGADGKTTTLQMVQ
                     ELEKAFERNLDTLAWMDAPTREQALVKVRKIVNKIGYPDKWRDYGAMKVDRESFLHSIM
                     GANAFEQAWQIAKIGKPVDKEEWLMSPPTVNAYYNPPFNEIVFPAGILQPPFFDREATM
                     PVNFGAMGMVVGHEITHGFDDEGRQYDAEGNLRDWWTPASDKAFRERVACVKEQYDNYT
                     VLDDVKVNGALTLGENVADLGGLKLAHAAMEAWLAKDPEAAKKAEGSRYTPSQQFFLSY
                     AQSWCSKYRDAYARQLAVVDPHSPPYWRVNGPVGNLSEFQKAFQCKADAKMVRPAAQRC
                     EVW"
     gene            complement(2528..3088)
                     /locus_tag="CYFUS_RS33745"
                     /old_locus_tag="CYFUS_006674"
     CDS             complement(2528..3088)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624021.1"
                     /locus_tag="CYFUS_RS33745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_006674"
                     /product="chalcone isomerase family protein"
                     /protein_id="WP_095988967.1"
                     /transl_table=11
                     /translation="MKNTTLCAVLLTAVLALPAAAKDVAGVAFPDTVSVEGKELKLNGV
                     GLRKKLVFNVYAAGLYLQNPSREGAQAIGSEQLKRVRMSMLRDLDKKTISEAIVDGFKK
                     NAKDKLPSLQQRLDTFTSAIPDLKKGDELLLTYVPGQGTTIESRAGQKISVEGKDFADA
                     LFSVWLGNNPVDGGVKDGMLGKE"
     gene            complement(3122..4210)
                     /gene="serC"
                     /locus_tag="CYFUS_RS33750"
                     /old_locus_tag="CYFUS_006675"
     CDS             complement(3122..4210)
                     /EC_number="2.6.1.52"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_5 (3-350). E-value: 5.2e-41.
                     Score: 132.7. Matches aSDomain:
                     nrpspksdomains_CYFUS_RS33750_Aminotran_5.1"
                     /codon_start=1
                     /gene="serC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554926.1"
                     /locus_tag="CYFUS_RS33750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_006675"
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="WP_095988968.1"
                     /sec_met_domain="Aminotran_5 (E-value: 4.5e-40, bitscore:
                     136.9, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVINFNPGPAGLPLPALERARDELIDFQGSGMSVMEHSHRGKEY
                     EAVHDETISLLTELLGIPDTHQVLFLTGGASQQFAQVPMNFLHPGTSADYLMTGVWSEK
                     AYDEARIVGQARIAATTVGPDKRYVRVPRQEELQLDPRAAYVHLTSNNTIYGTQWHTWP
                     EVGSVPLIADMSSDFMWKPMDVSRFAFLYAGAQKNLGPSGVLIAIARKDFIAQGRRDIP
                     KIFRYSLHAENNSLYNTPPTLAIYLCRNVLAWMKAQGGLAGLEARNRQKGELLYRALDA
                     NAGFYRAPVERESRSFMNVVFHLPTPELDAAFVSEAKKAGMVGLKGHRICGGIRASLYN
                     AVSVDDVRALVSFLEEFARKKG"
     aSDomain        complement(3161..4201)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CYFUS_RS33750_Aminotran_5.1"
                     /evalue="5.20E-41"
                     /label="CYFUS_RS33750_Aminotran_5.1"
                     /locus_tag="CYFUS_RS33750"
                     /protein_end="350"
                     /protein_start="3"
                     /score="132.7"
                     /tool="antismash"
                     /translation="INFNPGPAGLPLPALERARDELIDFQGSGMSVMEHSHRGKEYEAV
                     HDETISLLTELLGIPDTHQVLFLTGGASQQFAQVPMNFLHPGTSADYLMTGVWSEKAYD
                     EARIVGQARIAATTVGPDKRYVRVPRQEELQLDPRAAYVHLTSNNTIYGTQWHTWPEVG
                     SVPLIADMSSDFMWKPMDVSRFAFLYAGAQKNLGPSGVLIAIARKDFIAQGRRDIPKIF
                     RYSLHAENNSLYNTPPTLAIYLCRNVLAWMKAQGGLAGLEARNRQKGELLYRALDANAG
                     FYRAPVERESRSFMNVVFHLPTPELDAAFVSEAKKAGMVGLKGHRICGGIRASLYNAVS
                     VDDVRAL"
     gene            complement(4288..5004)
                     /locus_tag="CYFUS_RS33755"
                     /old_locus_tag="CYFUS_006676"
     CDS             complement(4288..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624019.1"
                     /locus_tag="CYFUS_RS33755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_006676"
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="WP_095988969.1"
                     /transl_table=11
                     /translation="MKPGLRGFFESMEAYFTDPGPEALERLYSTHPGWDAPRSRVALYG
                     QMVRHHGEATLDKLYPRVRGCVEPALWAELVRAYGASRPHRPFEMNRLGEGFAGFLADE
                     AGARGLPDFLPALARFEWTDFAVYTSMEPVPARVERLTVNPTLMMLEHPFRLCAFMRAG
                     GGQRPEPGQELALLWRHPERLVTMYLEAEERSLLVLKMAVEGLEPHQVAEATGVEEAVI
                     RAAVDTRVADGLVLAP"
     gene            complement(5001..5846)
                     /locus_tag="CYFUS_RS33760"
                     /old_locus_tag="CYFUS_006677"
     CDS             complement(5001..5846)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614260.1"
                     /locus_tag="CYFUS_RS33760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_006677"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_095988970.1"
                     /sec_met_domain="DUF692 (E-value: 4e-101, bitscore: 336.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVAHARRWGGEPLGVGIGLRREFYARLPETPRALDWVEIVPENF
                     LTLGGRPQRALDACRERWPVLPHGVALNVGGPEPLDEAYLAGLQALVERLDAPFFSDHL
                     CYARLGGAYLYDLLPLPFSPEAVEHVAPRVREVQARVGRPFLLENPSYYARMPGGTLAE
                     ADFLRHVAEEADCGLLLDVNNVYVNACNHGYEPRAFVDALPLERVGQIHLAGHERRPDV
                     LIDTHGAPVCDEVWSLYRYVLERTGPVPTLIEWDQDIPSLEAVLDEADRARALLQGVAR
                     "
     gene            complement(5956..6213)
                     /locus_tag="CYFUS_RS33765"
                     /old_locus_tag="CYFUS_006678"
     CDS             complement(5956..6213)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395329.1"
                     /locus_tag="CYFUS_RS33765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_006678"
                     /product="hypothetical protein"
                     /protein_id="WP_095988971.1"
                     /transl_table=11
                     /translation="MNLKTLTALVGSLSLGTLATGCATAKTAAAPAEQESTSAHAASAV
                     PAESSTAPAAEGTATPSTEKSSEHSCGSGGCGANGCGGKK"
     misc_feature    6574..6587
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.91"
                     /tool="antismash"
     gene            6673..8211
                     /locus_tag="CYFUS_RS33770"
                     /old_locus_tag="CYFUS_006679"
     CDS             6673..8211
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624015.1"
                     /locus_tag="CYFUS_RS33770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_006679"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_095992391.1"
                     /transl_table=11
                     /translation="MNENALNANVGLKLRGLRLARNIKQTDAAKDLGVSPAYLNLIEKG
                     KRVMPFPLLWKALRYFDQDPEQFMSTLGEGRVDEALAKLLDEPLLKSLDIDSESLQSLS
                     AEPKLAGTVAALFNLYKNTRTQLENVLAQLNSEERSRMNAAPGSSEGPRFDYSPFDEVS
                     DFLESHHNYFPELEEQAEALRRDFKLGRLLSSPQLVPMLEERFGYRVRLEAASSGSSVV
                     RRLDPDNQELILSPDLTEQPLKFQLAASIGLLMLDQEKLVERIVGAVRTRHAETLRLIK
                     VNLANYFAGALMLPYGEFFKEVERTRYDVELLSNLFGTTYETVAHRLCNLSDPKRRGLP
                     FHFLRADIAGNISKRYSGTGIKFATGGGSCGKWAVHLAFLNPSQITRQYSMMPDGTSYF
                     CFAKVQLQPIEGSIVRGTAYSIGLGTHAENAKYLAYGLPTTDLRKDAVPSGISCRFCER
                     TDCNQRAAASYRFAFSFDEYTKKDCFFSPLLVHEAGRAESLDKAPRRRNKAEEN"
     gene            8226..9101
                     /locus_tag="CYFUS_RS33775"
                     /old_locus_tag="CYFUS_006680"
     CDS             8226..9101
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554937.1"
                     /locus_tag="CYFUS_RS33775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_006680"
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_095988972.1"
                     /transl_table=11
                     /translation="MNHHDTERAHILQAIQDQRNALAVTRITGDPAETGRGLVHLAELH
                     GMLEEHAESRRHYEEALGFFQTAGDKEGQAQVLFGLGVARAHFGDHRGAVEHIAQATLL
                     FNGLKDQENEALCRAAIGESLRAIGQPKGAEEKFQEALLLYRQAKNGPRVARLLLDIGD
                     IRMEAGEYEAARKRFAESLQVLRKEPEIDMEALALCELLLGEAEGLLGNHEAARPHLQQ
                     AAELYSQLHDHAYESRARWDLSIACTFMQDWKGARTELETVIPLFEHQGRGEDVAKARK
                     VLAHFDSRGV"
     gene            complement(9273..10424)
                     /locus_tag="CYFUS_RS33780"
                     /old_locus_tag="CYFUS_006681"
     CDS             complement(9273..10424)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935195.1"
                     /locus_tag="CYFUS_RS33780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_006681"
                     /product="IS4 family transposase"
                     /protein_id="WP_095991839.1"
                     /transl_table=11
                     /translation="MRKPRLDDQQVHTFLESLFEEDLHAKRVLSLAYAVLGVIHAASLG
                     VHLIGKALAWARGTKSKHGVKQVDRLLSNQGIDVWALFAQWVPFALGQRSEALVALDWT
                     DFDADGQTTLVASLVASHGRTTPLVWLTVEKSALEGMRNDVEDFVLNRLRQVVPERVRL
                     TLLADRGFGDQKFYALLEQLKFDYVVRFRQCIQVMDETGEKKSAGEWVPESGRAVRMVG
                     ARVTQDEAPVGAVVCVKQKGMKEAWCLATSLKEATAAFVVGLYGKRFRTEETFRDMKDL
                     RFGMGLSSVRVRSTERRDRLLLVSALACALLTLLGAAGESLGMERYLKANTVKTRTYSL
                     FRQGCEYYQAIPMMPEEQLVPLMKRFASLLREQPVFQEFLGPI"
     misc_feature    complement(9315..9317)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 gggcatcttc gagcagcgtc tgcgcgagaa gctccccctg cgcgccgagc gcgtcctgca
       61 ccgcatccgc gagacgcgcg gtggcgagct caacgactcg cgcttccgcg tccgcatgcg
      121 tggggagggc ctctacgcgg agaccctcca ccagctcttc tcctccaccg cgcgccgtct
      181 gggcctgagg acgagcgagc ccatggaggc ggacagcgac aaggagagcc cctttcgccg
      241 ccccatgaag tccggcaccc agctcagctt cttctgagcg tcccgccgga cccgcccgag
      301 cgcctaccag acctcacagc gctgcgccgc gggacgcacc atcttcgcgt ccgccttgca
      361 ctggaaggcc ttctggaact cggacaggtt gcccaccggc ccgttcaccc gccagtacgg
      421 cggcgagtgc ggatccacca ccgcgagctg ccgcgcgtag gcgtcccggt acttcgagca
      481 ccaggactgc gcgtaggaga ggaagaactg ctggctcggc gtgtagcgcg agccctccgc
      541 cttcttcgcg gcctcgggat ccttcgccag ccaggcctcc atggccgcgt gggccagctt
      601 cagcccgccc aggtccgcca cgttctcgcc cagcgtcagc gccccgttca ccttcacgtc
      661 gtcgagcacc gtgtagttgt cgtactgctc tttcacgcac gccacgcgct cgcggaaggc
      721 cttgtccgag gccggcgtcc accagtcgcg caggttgccc tcggcgtcat actgccggcc
      781 ctcgtcgtcg aagccgtggg tgatttcgtg gcccaccacc atgcccatgg ccccgaagtt
      841 caccggcatc gtggcctccc ggtcgaagaa cggcggctgg aggatgcccg ccgggaagac
      901 gatctcgttg aagggcgggt tgtagtaggc gttcaccgtg ggcggagaca tcagccactc
      961 ctccttgtcc accggcttgc caatcttggc gatctgccac gcctgctcga aggcgttcgc
     1021 gcccatgatc gagtgcagga acgactcgcg atccaccttc atcgcgccgt agtcgcgcca
     1081 cttgtccggg tagccgatct tgttgacgat cttgcgcacc ttcaccagcg cctgctcgcg
     1141 cgtgggcgcg tccatccacg ccagcgtgtc caggttgcgc tcgaaggcct tctcgagttc
     1201 ctgcaccatc tgcagcgtgg tcgtcttgcc gtccgcgccg aacgtgcgct cgatgaaggg
     1261 ctgcgccagc gcctcgccca gcgccatgtt ggtgaagcgc acgcacttct tccagcgcac
     1321 cacgtcctcc ttcgcgcccg tcaggttctg cgagaagcgg aagcgctcgt cctggaaggc
     1381 cttgggcagc gccggcacca cgctcgacac gtagttccac gtcaggtacg gcgcccacgc
     1441 cgccgccggc atcgtcttcg ccagccgctc cacctccttg aagaacggtg ggtgcgacac
     1501 gttgagcgcc tgcacgctcc cggcccccgc ggccttgaag tactcgtccc acgggaagcc
     1561 cggtgcctct gccttcaggc ccttgcgctc gaggcggtgg tagagactct tcggctcgcg
     1621 gcgctccacg cgcgacatcg acgccttggc gagcgccgtc tccacctcca gcaccgacgc
     1681 cgcgctcttc gccgcggtct cggccggctc gcccagcagc acgaaggtgt tcttcacgta
     1741 ctcggtgtag gcgtcacgca gcgccttcga cttctcgtcg tccttcaaat agtagtcccg
     1801 gtccggcagg cccagaccgc cctggtccac ctcgccgatc atctggctgg cgtccttgaa
     1861 gtcggtgttg gcgcccatcc ggaagagcgg gaacacgttc tgcgcgtgca gcgtgcccac
     1921 cacccgcgcc aggtccttcg cgttcttcac cgtcagcttc gccagctccg ccttgagcac
     1981 cggcagcgac gcctccagct tcgcctcatc catgcaggag ccgtagaagt cccccagctt
     2041 ctgcgcggag gcgagcccct cgggcgcctt gcccttggac gcgccctcga ggatctcccg
     2101 gaggatctcc tcgttgcgcg ccgagatggc atcgaagccg cgcgaccagc gggggcgatc
     2161 cgcggggatc tccgtgttct tcacccagtt gccgcatgcg aactcgtaga agtcctcgca
     2221 ggggttggcc tgctcgttca tcgaggccag atccagacct ggggagagcg gacgggactc
     2281 ggcggggggc tggggctgcg tcgcgggcgt ggcgggcgag gggggcgtgg gcgcgggctc
     2341 cgccgtcttg caagcggacg ccacaagaca acatgcggcg agcaatgcct tcttcatcca
     2401 gtcgttctcc atgtggggtg tgaggcagcg cgcaccctat ccggccacgg gcacgcgcgc
     2461 ctcaacctcg tacgggaggg ccaccctcac gcccttcccg ttgcacgacc cccgacatgg
     2521 agtcctgcta ctccttgccc aacatcccgt ccttgacacc gccgtcgacc gggttgttgc
     2581 cgagccacac cgagaagagc gcgtcggcga agtccttgcc ctccaccgag atcttctgtc
     2641 ccgccctgct ctcgatggtg gtgccctggc cgggcacgta cgtgagcagc agctcgtcgc
     2701 ccttcttcag gtccggaatg gccgaggtga aggtgtccag ccgctgctgg agcgagggca
     2761 gcttgtcctt cgcgttcttc ttgaagccat ccacgatggc ctcggagatc gtcttcttgt
     2821 ccaggtcgcg cagcatcgac atccgcacgc gcttgagctg ctccgagccg atggcctggg
     2881 cgccctcgcg cgaggggttc tgcaggtaga ggccggcggc gtacacgttg aagacgagct
     2941 tcttgcgcag gcccaccccg ttgagcttca gctccttgcc ctcgacggac accgtgtcgg
     3001 gaaatgccac ccccgccacg tccttggcgg cggcgggcag cgcgagtacg gcggtcaaca
     3061 acacggcaca cagcgtggtg ttcttcatga cctctcctgt ggaaggggtg ggggggccac
     3121 cctagccttt cttccgggcg aactcctcca ggaaggacac cagggcgcgc acgtcgtcca
     3181 ccgacaccgc gttgtacagg gaggcgcgga tgcccccgca gatgcggtgt cccttgaggc
     3241 ccaccatgcc cgccttcttc gcctcggaga cgaacgcggc gtccagctcc ggggtgggca
     3301 ggtggaagac cacgttcatg aaggagcgcg actcgcgctc cacgggcgcg cggtagaagc
     3361 ccgcgttcgc gtccagggcg cggtagagca gctcgccctt ctgccggttg cgcgcctcca
     3421 gccccgcgag cccgccctgc gccttcatcc acgcgagcac gttgcggcac aggtagatgg
     3481 ccagcgtggg gggcgtgttg tagagcgagt tgttctccgc gtggagggag tagcggaaga
     3541 tcttcgggat gtcccggcgg ccctgggcga tgaagtcctt gcgcgcgatg gcgatgagca
     3601 cgcccgaggg ccccaggttc ttctgcgccc ccgcgtagag gaaggcgaag cggctcacgt
     3661 ccatgggctt ccacatgaag tccgagctca tgtccgcgat gagcggcacg ctgcccacct
     3721 cgggccaggt gtgccactgg gtgccgtaga tggtgttgtt gctcgtcagg tggacgtacg
     3781 ccgcccgggg atccaactgg agctcctcct ggcggggcac gcgcacgtag cgcttgtccg
     3841 ggcccacggt ggtggcggcg atgcgcgcct gacccacgat gcgcgcctcg tcataggcct
     3901 tctcgctcca cacccccgtc atcaggtagt ccgcgctcgt gcccgggtgc aggaagttca
     3961 tcggcacctg ggcgaactgc tgcgacgcgc cccccgtgag gaagagcacc tggtgggtgt
     4021 ccggaatgcc cagcagctcg gtgagcaggg agatggtctc gtcgtggacc gcctcgtact
     4081 ccttgccccg gtggctgtgc tccatcacgg acatgcccga gccctggaag tcgatcagct
     4141 cgtcgcgcgc tcgctcgagc gcgggcaggg gcagccccgc ggggccggga ttgaagttga
     4201 tgacgcgcat ggctgggtcc tctcactggg aaaacaacgc gcggccattc tgcccaacgc
     4261 ctccgccagg ggaagcgcga agcgccctca gggcgccagg acgagaccgt cggccacccg
     4321 ggtgtccacg gcggcacgga tgaccgcctc ctccacgccc gtcgcctccg ccacctgatg
     4381 gggctccagc ccctccaccg ccattttcaa caccaacaac gagcgctcct cggcctccag
     4441 gtacatcgtc accaggcgct ccgggtgacg ccagagcagc gccagctcct gtccgggctc
     4501 cggccgctgc cctcccccgg cccgcatgaa cgcgcacagc cggaagggat gctccaacat
     4561 catcagcgtc ggattcaccg tcaggcgctc cacgcgcgcg ggcacgggct ccatcgacgt
     4621 gtacaccgcg aagtccgtcc actcgaagcg cgccagcgcg ggcaggaagt ccggcagccc
     4681 ccgagccccc gcctcgtccg cgaggaagcc ggcgaagccc tcgcccaggc ggttcatctc
     4741 gaagggccgg tgaggacgcg aggcaccgta ggcccgcacc agctccgccc agagcgccgg
     4801 ctccacgcat ccacgcaccc gcgggtagag cttgtccagg gtggcctcgc catggtggcg
     4861 caccatctgg ccatagagcg ccacgcgcga gcgcggcgcg tcccagcccg ggtgcgtcga
     4921 gtacagccgc tcgagcgcct ccggccccgg gtccgtgaag tacgcctcca tcgactcgaa
     4981 gaagccacgc agtcccggtt tcatcgcgcc accccctgga ggagcgcccg cgcccgatcc
     5041 gcctcgtcca gcaccgcctc cagcgagggg atgtcctgat cccattcgat caacgtcggc
     5101 accggcccgg tgcgctcgag cacgtagcgg tagagcgacc acacctcatc gcagacgggt
     5161 gccccatgcg tgtcgatgag gacgtcgggc cgccgctcgt gccccgcgag gtggatctgc
     5221 cccacgcgct ccagcggcag cgcgtccacg aaggcgcgcg gctcatagcc atggttgcac
     5281 gcgttcacgt acacgttgtt cacgtccagc aagaggccgc agtcggcctc ctcggccacg
     5341 tgccgcagga agtccgcctc cgccagggtg ccgccgggca tgcgcgcgta gtagctcggg
     5401 ttctccagca ggaagggccg gcccacgcgc gcctgcacct cgcgcacccg cggcgccacg
     5461 tgctccaccg cctcgggcga gaagggcagc ggcagcagat cgtagaggta ggctcccccc
     5521 agccgcgcgt agcagaggtg atccgagaag aagggcgcgt ccagccgctc caccagcgcc
     5581 tggagcccgg cgaggtaggc ctcgtccagt ggctccggac cgcccacgtt cagcgccacc
     5641 ccatgcggca acaccggcca gcgctcgcgg cacgcgtcca gcgcacgctg tggacggccg
     5701 ccgagcgtga ggaagttctc cgggacgatc tccacccaat ccagcgcacg gggggtctcc
     5761 ggcagccgcg catagaactc gcgccgcagc ccgattccca ccccgagcgg ctcgccgccc
     5821 caccttcgcg cgtgtgccac cggcatgtcg gctcctcgca gaaaagacac gggcggcggg
     5881 aagaaaagcc ccaccgcccg ggagacaacc cccggagcac cgggggccat ccttgaagga
     5941 aggcttcttc gacggctact tcttgccacc acagccgttg gcgccgcagc cgcccgagcc
     6001 acagctgtgc tcactggact tctcggtcga gggcgtcgcg gtgccctcgg cggcgggggc
     6061 ggtggaggac tcggcgggga cggcgctggc cgcgtgagcg ctcgtgctct cctgctcggc
     6121 cggagcggcc gcggtcttgg cggtcgcaca gccggtcgcg agcgtgccga gggagaggga
     6181 gcccacgagg gcggtcaggg tcttgaggtt catggcgttt cctttcaggg gtcgggccgg
     6241 ggtcggatgc acctggcgga gaactcccct cgcccggaga cgaagggagc ccgcgccacc
     6301 atacgcccgg cgcccgatgg ggttacaccg gggagaacac ggctttggga attccatgcc
     6361 cgtgcgacgc ccaccctctc gggagttgct gggttttccg cctccctgtc caggtggcgg
     6421 taaacgaggt gaatggaatt tgacagcccg cccgctccct tggaccccag tccatggagc
     6481 ccaaaaaccc cctgttgaat caatggcttg gaatgtcgaa aacgggcgag gcgtctgcct
     6541 tgagggactc ccggagcctc gggagtccgg gatattttct ccgaaaacgc cgattgcaaa
     6601 tcagtaaacg cgctcgcaca gttcgaggaa cggaggagcc ggcgggccat gggcgccggc
     6661 ccagagatgg cgatgaacga gaacgcactg aacgccaacg tgggactgaa gctgcgcggc
     6721 ctgcggctcg cccggaacat caagcagacg gacgcggcga aggatctggg ggtgtccccg
     6781 gcgtacttga acctcatcga gaagggcaag cgcgtcatgc ccttcccgct cttgtggaag
     6841 gcgctgcgct acttcgatca ggatcccgag cagttcatgt ccacgctggg tgagggccgg
     6901 gtggacgagg cgctcgccaa gctgctggac gagccgctgc tcaagagcct cgacatcgac
     6961 tccgagtccc tgcagagcct gtcggcggag ccgaagctgg ccggcacggt ggcggcgctc
     7021 ttcaacctct acaagaacac gcgcacgcag ttggagaacg tgctcgcgca gctcaactcc
     7081 gaggagcgct cgcgcatgaa cgcggcgccg ggcagctcgg aggggccgcg cttcgactac
     7141 tccccgttcg acgaggtgag cgacttcctc gagtcgcacc acaactactt ccccgagttg
     7201 gaggagcagg ccgaggcgct gcggcgcgac ttcaagctcg ggcgcctgct gtcgagcccc
     7261 cagctcgtcc ccatgctgga agagcgcttc ggctaccgcg tgcgcctgga ggccgcctcc
     7321 agcggctcct cggtggtgcg ccgcctggat ccggacaacc aggagctcat cctctcgccg
     7381 gacctgaccg agcagcccct caagttccag ctcgccgcct ccatcggcct gctgatgttg
     7441 gatcaggaga agctggtgga gcgcatcgtg ggcgcggtgc gcacccggca cgcggagacg
     7501 ctgcggctca tcaaggtgaa cctggccaac tacttcgccg gcgcgctgat gctgccctac
     7561 ggggagttct tcaaggaggt ggagcgcaca cgctacgacg tggagctgct gtccaacctc
     7621 ttcggcacca cgtacgagac ggtggcgcac cggctgtgca acctgtcgga ccccaagcgc
     7681 cgcggcctgc ccttccactt cctgcgcgcg gacatcgccg gcaacatctc caagcgctac
     7741 agcggcaccg gcatcaagtt cgccaccggc gggggctcgt gcggcaagtg ggccgtgcac
     7801 ctggccttcc tcaacccgtc gcagatcacc cggcagtact cgatgatgcc ggacggcacg
     7861 tcctacttct gcttcgccaa ggtgcagttg cagcccatcg agggctccat cgtgcgcggc
     7921 acggcctact ccatcggcct gggcacgcac gcggagaacg ccaagtacct ggcgtacggg
     7981 ttgcccacca cggacctgcg caaggacgcc gtccccagcg gcatctcctg ccgcttctgc
     8041 gagcgcaccg actgcaacca gcgcgcggcc gccagctacc gcttcgcctt ctccttcgac
     8101 gagtacacca agaaggactg cttcttctcg cccctgctgg tccacgaggc cggccgtgcg
     8161 gaatcgctgg acaaggcgcc gcgccgccgt aacaaggccg aggaaaacta gcccccccgc
     8221 cctccatgaa tcaccacgac accgaacgcg cccacatcct ccaagccatc caagaccagc
     8281 gcaacgcgct cgccgtgacg cgcatcaccg gagacccggc ggagacgggc cggggactcg
     8341 tccacctggc cgagctgcac ggcatgctgg aggagcacgc cgagagccgc cgccactacg
     8401 aggaggccct gggcttcttc cagacggcgg gggacaagga gggccaggcc caggtgctct
     8461 tcgggctcgg ggtggcacgc gcccacttcg gggatcaccg gggcgccgtg gagcacatcg
     8521 cccaggccac gctgctcttc aacggcctga aggatcagga gaacgaggcg ctgtgccggg
     8581 cggccatcgg agagtcgctg cgagcgattg gccagcccaa gggcgccgag gagaagttcc
     8641 aggaggcgct gctgctctac cggcaggcga agaacgggcc ccgggtggcc cggctgctgt
     8701 tggacatcgg cgacatccgc atggaggcgg gcgagtacga ggccgcgcgc aagcgcttcg
     8761 ccgagtcgct gcaggtgctg cgcaaggagc cggaaatcga catggaggcc ctggcgctgt
     8821 gcgagctgct gctgggcgag gccgagggac tcctgggcaa ccacgaggcg gcgcggcccc
     8881 acctccagca ggccgcggag ctgtactcgc aactgcacga ccatgcctac gagtcgcggg
     8941 cgcggtggga tttgagcatc gcgtgcacct tcatgcagga ctggaagggc gcgcggaccg
     9001 agctcgagac ggtcatcccg ctcttcgagc accagggccg cggagaagac gtggccaagg
     9061 cccgcaaggt gctcgcccat ttcgactcgc gcggggtgta gggccccggg cggagcggcc
     9121 tgtgctcacc acccactcga gtggtgagca cacccaggct agaaatccga acccatgtca
     9181 tttctgagaa tacagtccgg acatcggaat gacatacaat cacaattttg acaccaactc
     9241 cgtctttgaa atcctgcgtc tgacccatcc cctcatattg ggccgagaaa ttcctgaaaa
     9301 actggctgtt cacgtaacag cgaagcgaat cgcttcatca acgggacgag ctgctcctcc
     9361 ggcatcatcg ggatggcttg gtaatactca cagccctgcc ggaagagtga gtaggtgcgc
     9421 gtcttcactg tgttggcttt gaggtagcgc tccatcccca agctctcccc cgccgccccc
     9481 agcagcgtca gcaatgcgca ggccagcgcg ctcaccaaca gcagccggtc ccgcctctcc
     9541 gtcgagcgga ccctcaccga cgacaggccc attccaaaac gcaagtcctt catgtcgcgg
     9601 aatgtttcct ccgttcgaaa cctcttgcca tacagcccca ccacgaaggc cgccgtcgct
     9661 tctttcaagc tcgtcgccag gcaccacgcc tccttcattc ccttctgctt cacgcacacc
     9721 acagctccca cgggcgcttc gtcctgggtg actcgcgctc ccaccatgcg caccgcccgg
     9781 cccgattcgg gcacccactc gcccgcgctc ttcttctctc ccgtctcatc catcacttga
     9841 atgcactgac ggaagcgcac cacgtagtca aacttcagtt gctcgagcag cgcatagaac
     9901 ttctggtctc caaagccccg gtcggccagt agtgtgagcc gcacccgctc gggcaccacc
     9961 tgccgcagtc gattgagtac gaaatcctca acgtcattgc gcatcccctc caaggcggac
    10021 ttctccaccg tgagccagac caggggcgtc gtgcgtccat ggctcgccac caacgacgcc
    10081 accagcgttg tctgcccatc cgcatcgaag tccgtccagt ccagcgccac cagtgcctcg
    10141 cttctctgtc ccaaggcaaa gggcacccac tgtgcgaaca gcgcccagac atcaatccct
    10201 tggttggaca agagccggtc cacctgcttc accccatgct tgctcttggt gcctcgcgcc
    10261 cacgccaggg ctttgccaat gagatgaacc cccaggctgg cagcgtgaat gacacccagg
    10321 accgcgtagg ccagggacaa cacgcgcttg gcgtgcaggt cttcttcgaa gagggactcc
    10381 agaaaggtat gcacctgctg gtcatcaagg cgaggcttac gcatgaccgc agaagtaagc
    10441 atgcgggtcc tacttctcgc actactcggc ctacgcggcg ctcccttgct cgcctgttaa
    10501 aatgagggga tggctcagat cctgcgtacc atcaacgaaa ggtatccaga gggatctcct
    10561 gaagacgaag cactccgaat cgccgcagtg gctcttgtct acgttcgcga aacccagaaa
    10621 ttggatgact atcgcgagtt ctttcgatcg ttctacactc cagccattga ctacgtcgtc
    10681 atttcccaaa cgttctcgac gagagaagaa gccgacatat ggctggcagg tggaacggca
    10741 agggacggag atctcgtcag gatcgcgggg caaggcttcc aagtcatgcc gcagcgaaaa
    10801 ggaacgggac tccgattcat caggatgccc ctgcccgaag aactga
//
