LOCUS       NZ_CP022098            10639 bp    DNA     circular CON 17-FEB-2024
DEFINITION  Cystobacter fuscus strain DSM 52655 chromosome, complete genome.
ACCESSION   NZ_CP022098
VERSION     NZ_CP022098.1
KEYWORDS    RefSeq.
SOURCE      Cystobacter fuscus
  ORGANISM  Cystobacter fuscus
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Archangiaceae; Cystobacter.
REFERENCE   1  (bases 1 to 12349744)
  AUTHORS   Rupp,O., Goesmann,A. and Sogaard-Andersen,L.
  TITLE     Sequencing and comparative analysis of myxobacterial genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12349744)
  AUTHORS   Bruckskotten,M., Treuner-Lange,A., Rupp,O., Goesmann,A. and
            Sogaard-Andersen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUN-2017) Department of Ecophysiology, Max Planck
            Institute for Terrestrial Microbiology, Karl von Frisch Str. 10,
            Marburg, Hessen 35043, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2016
            Assembly Method                   :: Celera Assembler v. 8.1
            Expected Final Version            :: yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 10:04:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,093
            CDSs (total)                      :: 10,016
            Genes (coding)                    :: 9,906
            CDSs (with protein)               :: 9,906
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 26 of 110
            Pseudo Genes (incomplete)         :: 101 of 110
            Pseudo Genes (internal stop)      :: 1 of 110
            Pseudo Genes (multiple problems)  :: 17 of 110
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:24:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7095502
            Orig. end                         :: 7106141
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10639
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="36"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10639
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10639
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5639](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5639
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    1244..1258
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.35"
                     /tool="antismash"
     misc_feature    1634..1645
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     gene            2740..3828
                     /locus_tag="CYFUS_RS28895"
                     /old_locus_tag="CYFUS_005711"
     CDS             2740..3828
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CYFUS_RS28895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CYFUS_005711"
                     /product="hypothetical protein"
                     /protein_id="WP_095988159.1"
                     /transl_table=11
                     /translation="MKPASISPARIRQFGDPEDAAFCLVTNREFISQIHLEREGGYSDY
                     LILEYENGDRFSELLAKKIPEHAHILVISPNAFFQSPRAEELGSRRKLLAMACNSTPTA
                     WDMIPRFLKIVEDTDPEEQQRFTDRFFAMGEASAYLEFVDERYGTRARLEHLDERYLWN
                     EQAGLLNWGGQQLAPSGEISVLPLHIWDFDSNLYLKFNGTIAIQGYPILHNGTPSFLRA
                     DQARIHGQLACLSREALIATVENGRIVDLKAAGPEAQPAHDMMTAMFEVDSRYRIIWEL
                     GFAINTHSTIVPGNFAMNEVFGAENGTIHFGLGLTPHTQYHLDLICPGLTVLTDKGQVV
                     LGRRGPVGAMPRVRAEGCACLE"
     misc_feature    3016..3018
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3828..4517
                     /locus_tag="CYFUS_RS28900"
                     /old_locus_tag="CYFUS_005712"
     CDS             3828..4517
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /locus_tag="CYFUS_RS28900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_005712"
                     /product="AzlC family ABC transporter permease"
                     /protein_id="WP_095988160.1"
                     /transl_table=11
                     /translation="MLQSRTGLARSISVGLALFPVGLLFGLTAAQNGWRPMEVFALSAI
                     GFTGSGQFALLALTKEGVAPLLIFLIILLINLRYVPMAFTAARGMDGAVGKRATLAHFV
                     SDESYAVEQIADPPAVRASIRLWIALFWVASTTAGALVAMLLPAHWAGSIGAVTFPANA
                     LLILLALLRVRAFVERLGRRGLAAILASLPIALGIRILLGSKLFWLPSILLVGLTIHWI
                     CKARDAS"
     gene            4514..4831
                     /locus_tag="CYFUS_RS28905"
                     /old_locus_tag="CYFUS_005713"
     CDS             4514..4831
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CYFUS_RS28905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CYFUS_005713"
                     /product="hypothetical protein"
                     /protein_id="WP_095988161.1"
                     /transl_table=11
                     /translation="MTTTAWIGITLLLGVSLVVRVFPALLRPPLSQATLEQIETILPIA
                     VLINLAIFSASSEIAAAPGPALLGLGILGVLTFAARTVPILVTLIASTTAFLLLLTALN
                     R"
     gene            5001..5639
                     /locus_tag="CYFUS_RS28910"
                     /old_locus_tag="CYFUS_005714"
     CDS             5001..5639
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015700.4"
                     /locus_tag="CYFUS_RS28910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_005714"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_157758713.1"
                     /sec_met_domain="AfsA (E-value: 8.8e-23, bitscore: 79.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLIEDGVDGRDTPGVVDEVPAGVLEKLKEWVKTFDPSARLQLPRH
                     LVHKSDPRNVFIARLERVSEARPDELAAQLIFDPTHPYHFEHPQDHVPGMMLMEAGRQL
                     GLAVTHLFYGVALDAMFVLNDVKARFRRFAELGEPVFVYSAVHEKEYRRGRLISMRQAG
                     LFIQRGEFLGSMEGSWSVYDRRLMERLRRRPRPGDGSATSRPMTAPATE"
     gene            5645..6946
                     /locus_tag="CYFUS_RS28915"
                     /old_locus_tag="CYFUS_005715"
     CDS             5645..6946
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /locus_tag="CYFUS_RS28915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_005715"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_157758714.1"
                     /sec_met_domain="DAO (E-value: 9.5e-07, bitscore: 27.9,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKHYVVVGGGVSGIAAAHYLREAGAEVELVEQEDTLGGRVAPAL
                     LDGQPIELGGKNIGRRYLLFREFARKMGQTDFEPFGINSSRVRPDGRLMTVDSSHRWRG
                     VRELMRACPSSDLLRFATLCARAISRPENGYLGSVRFDALGEHRDDRPLSAYFSQAFSE
                     AVLRPMVVRMNGAEPDEVFPGNVGTNLRMLLDTYEQPKRGMRALFDRFARTVRVTRGTR
                     ARGLVVRGGRVAALELEGPGGAREERTCDGVLLALPAHPSAALLAPHAPGLALALGHVR
                     YFPVLVVVARYARKVFSPSTRALVFGPEHPLSNAGAYGINALDVVRYTFSGRTARRALA
                     AGTDVEALLRVGEKLLGHHFPVHPAERVASVARRFEPGLCAYTPHHARLRRELNAGLGR
                     LHGLQLAGDYLRGASIEACFRAAKESAGRMLRNR"
     gene            6994..8469
                     /locus_tag="CYFUS_RS28920"
                     /old_locus_tag="CYFUS_005716"
     CDS             6994..8469
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /locus_tag="CYFUS_RS28920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_005716"
                     /product="radical SAM protein"
                     /protein_id="WP_157758715.1"
                     /sec_met_domain="PF04055 (E-value: 2.4e-13, bitscore: 50.0,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKYYLGPEASHEVPFKRLLAISHYIMPNFGTLEWDRVSREQVDK
                     NLRSLQDPGGALNDELMMYIHVPYCQSFCHYCNFNKNHYPWQDEERLQRYTDYLIKEID
                     YYLSLPYVQARRFTAIYIGGGSPSTLPVSAVERLFAHLAKVAPGFDTLEKTFTGEPRTL
                     RKPELLKVLHDYGFDRVTFGIETLNPEIHKKIGRWDSPSDVDAVFEGLEKLGYEGDRCV
                     DLMYDLPGQSLVGFQEELATLVEHYRPDEIDAFGTVYLPYRPLHKLILDGRVPQPGSIW
                     QLLRMREHLYDYLLENDYHNTIAETYSRKPQRSMYQTAHCARQDIIGIGCAARSNIKDM
                     VSINPDKVDVWMNNIDQYGVSTQTLQSIGRSGVLDRIMVMFPRYKELSKELLDRYSDVE
                     HFEQVEDVLRSHLEVGCVEEQEGRYVVNKLGVIWHGNLQTDYMERSLNLQGKVLLKVIS
                     EKESHFDREERFKVNKATRFIAKHIDKYPKLMK"
     gene            8489..9400
                     /locus_tag="CYFUS_RS28925"
                     /old_locus_tag="CYFUS_005717"
     CDS             8489..9400
                     /NRPS_PKS="Domain: Abhydrolase_1 (32-266). E-value:
                     2.3e-19. Score: 61.9. Matches aSDomain:
                     nrpspksdomains_CYFUS_RS28925_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /locus_tag="CYFUS_RS28925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_005717"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_095988165.1"
                     /sec_met_domain="PF00561 (E-value: 4.6e-15, bitscore: 55.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 8.2e-12, bitscore:
                     45.4, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVDVVRRRELVPVEDGTRLELEVFEQVEERKAPVLLCLPAMGVPA
                     RYYEPFALELHRRGFHVVTSDLRGHGASSVRVGRGTDFGYYEMVARDLKAVVRAVRAAF
                     QDSPLFLLGHSLGGQLGSLYLGLEGEGIRGLVLVAASSVYYRNYGALGGVKVLLGTQLA
                     AVIATVWGYFPGRRLRFADNESTRVMRDWARQARRGRYVLSGAAQDFEALLARVERPVL
                     AISVEGDSLAPPAAVEHLYGKMPRARVVRWHFTRSDAGGAHLDHFRWVRHGASVAARIA
                     GWVGEVLAGEIGSGQDPQRGET"
     aSDomain        8585..9286
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CYFUS_RS28925_Abhydrolase_1.1"
                     /evalue="2.30E-19"
                     /label="CYFUS_RS28925_Abhydrolase_1.1"
                     /locus_tag="CYFUS_RS28925"
                     /protein_end="266"
                     /protein_start="32"
                     /score="61.9"
                     /tool="antismash"
                     /translation="PVLLCLPAMGVPARYYEPFALELHRRGFHVVTSDLRGHGASSVRV
                     GRGTDFGYYEMVARDLKAVVRAVRAAFQDSPLFLLGHSLGGQLGSLYLGLEGEGIRGLV
                     LVAASSVYYRNYGALGGVKVLLGTQLAAVIATVWGYFPGRRLRFADNESTRVMRDWARQ
                     ARRGRYVLSGAAQDFEALLARVERPVLAISVEGDSLAPPAAVEHLYGKMPRARVVRWHF
                     TRSDAGGAHLDH"
     CDS_motif       8807..8848
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CYFUS_RS28925_0001"
                     /evalue="6.30E-04"
                     /label="NRPS-te1"
                     /locus_tag="CYFUS_RS28925"
                     /protein_end="120"
                     /protein_start="106"
                     /score="12.4"
                     /tool="antismash"
                     /translation="SPLFLLGHSLGGQL"
     misc_feature    complement(10588..10599)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
ORIGIN
        1 cagagccccc ccggcccgaa gcctctccgc cccgatggct cgtccccacc ctgggcgtgg
       61 gactcaccct cctcttcttc catcgggccg tcttctccgc caacgtcttc ttcctgcgcg
      121 acgtgcagca agtctacgta ccgctgtggg aatactggcg cgagcgggtg ctcggggggg
      181 agtttcccca gtggtatccc ttcgatgggc tcgggcagcc ctacgtgggc atggtcgtct
      241 ccggcgcctt ccacccggcc caggtgctgt cgctggtgct acccggggcg cagggcctca
      301 agtggctcat gctgctgtgc ttcccggtgg cgttcctcgg catgagcgcg ctggggcggc
      361 ggctcgggtg ggggctcggg gccgcgctgc tcgcggcgat gagctacacc ttcagcggct
      421 acctcgtcag catcaccaac aacaccctgt acctcctggc ggccgtcacc gtgccctggg
      481 tgttctgggc gctggagggc tttctcaggg cacccacggt gggacggggg ctcggggtcg
      541 ccgtgctgct cgggctcgtg ctgctcgccg gcgacgtgca gggctacgcg gtctgtctgg
      601 gcggggccct gctgtggagc gtgagccgcc cggcgtccgg aggcacgccc cgcgcgctgc
      661 tggggatgct gggagtgggg gtcgtctccc tgctgctgtg cgcggtgcag atcctcccga
      721 cgctggcggg gctgggcgag gtgcgcgcct cgaatcagac gctggcgcag gcgacggagt
      781 ggtccgtcca tcccatgcgg ctggtggagc tggtgctcgg gcccctgttc gcgggcgatt
      841 ccggcgagcc cgtccaggtg gccatcagtg cgtggctgct gtccacgtcg aggaccgagc
      901 tgtgggcgga ctcgttctat ctcggcgcgc ccgcgctcgt gctggcgggc gtggcggtga
      961 cccgggtgcg ggggccgcgc gcggccttgc tgctcgccgc cgccctgggg gtggtgctgc
     1021 tggcgctcgg caagcacacg ccgctctacg gctgggtgtt cgattgggtg ccgccgtggc
     1081 gggccttccg ctaccccgag aagctgatga cgttcgtcac cttcacggtg tgcctggcgg
     1141 cgggcgtggg gtgggagcgg ctcgagcacg cgcccgacgt ccggcgccgg atggggcacg
     1201 gcgtgctcgg gctgggcgga ctggtgctcg cggtgggcgg cgcggagtgg ctcggccacg
     1261 tgttctccga ggggatgttg tccggcctgt ggatggggcc gccatggccc gaggcccagc
     1321 ggcgcatcgg cgagcacttc gtgctgggct gtgggctggc gggcgggagc ctgctcgcgt
     1381 tggggggcat cctcggcggg gtgcgctcgg cggaggcgcg ggccgggctc gtgggggtgg
     1441 tgggcttcgg ggggctgctg ctggccaatg gcgacatcta caaggtggga gagccggagc
     1501 tgttcgacga gccaacgccc ttcgtcctgc gcatccagga ggagctgcgc gagcagttcg
     1561 gtccgccgcc tcgggtgtac cggctgttgg gccgctacta cacgccgcac cccttgccac
     1621 tgcccacctt ggcggagacg aacgcgctca tcatgggcgc ctcactgatg ccgctcgtgc
     1681 cagcgctctt cggcctggag agctcgagcg cgtacatgcc cgccgtgtcg cgacgggtgg
     1741 cggacgtgca ggcggagacg agatcctggg tgcgtcgcac cgcgggcctg ttccacacgc
     1801 gctacttcat cctctcgagc gagtcgagcg aggtcaccgc actggtggcg gagccgctcc
     1861 gggtgctgga gcggctggac gccttcgggc tgtggctggt ggaggactcg aacgcgctgc
     1921 cacgcgcgta cctcgcccac ccgcgctgtg tgacggctcc gagagaggcg ctgcggcggc
     1981 tcgggagcat ccaggccccg cgcgaggtgg tggtggagtg cgcggcgccc ctgcccgagc
     2041 cgccgctgga ggcaccccgg ggcgaggtca ccgccgtccg atccctaccg gagcgggtgg
     2101 aggtggaggt gcaggcgcgg ggcggagagg tgctggtgct caacgacgcc tactacggag
     2161 gatggacggc gagcgtggac ggggagccgg tgcccatcct gcccgccaat ggggcggtgc
     2221 gcgcggtggc ggtgcccccg ggggcgcatc aggtggtctt ccgctaccgc acgcccgggc
     2281 tggccgtggg cgccggggtg agcctgggca cgctgctcgt gctgctcggc gtggatggag
     2341 tgctacggcg gcggctcacg ccaagggccg ggagaacaac gagtttctcg tgagccccga
     2401 gaggcggccg gagggcgcct ggccgcttgc gtccggcgga caagcgagac ccggggcctg
     2461 gcgaaggaga ccgtcggcgt ggccctggat gcccgcgaac tggaagccga tcacaaagcc
     2521 ggactcggtt ctccatccat ttgggccggt tttccaaatc ctccctaccg gaggagccgg
     2581 agcccgaatc acccacatgg aggccgtcat gaagaaccgt cgcaagaatg gtgagtacct
     2641 cggcagtctg ctgggctgaa gctggctatc aggccagggg gatgttcccc ctggcttgtt
     2701 tatttccagt ggccttgttt ttccctgaag gaactcgtca tgaagccagc ctccatctct
     2761 ccagcccgaa ttcgtcagtt tggcgatcca gaagatgccg ccttctgtct tgtgacaaac
     2821 agggagttca tcagccagat ccacctggaa cgagagggcg gatatagcga ctacctcatt
     2881 ctcgagtatg agaatgggga tcggttcagt gaattgctcg cgaagaaaat cccggagcat
     2941 gcgcacattc tggtgatctc ccccaatgcg ttcttccagt ctcctcgcgc cgaggagttg
     3001 ggcagtcggc gcaagttact cgccatggcc tgcaactcga cacccaccgc atgggacatg
     3061 atcccccgct tcctgaagat cgtcgaggat accgatccgg aagagcagca gcgcttcacg
     3121 gaccgtttct tcgcgatggg agaggcctcc gcctatctgg agttcgtcga cgagcggtat
     3181 ggcacgcgag ccaggctcga acatctcgac gagcgctact tgtggaacga gcaggcgggt
     3241 ctgctcaact ggggcggcca gcagctcgcc ccctcgggag agatcagcgt cctgccgctc
     3301 cacatctggg acttcgattc caacctctac ttgaagttca atggcaccat cgccatccag
     3361 ggctacccca tcctgcacaa tggcacgccc agcttcctgc gtgccgatca ggcccgtatc
     3421 catgggcagc tcgcctgcct ctcacgggag gcgctcatcg cgacggtgga gaacggcagg
     3481 atcgtcgatc tcaaggcagc ggggcccgag gcacagcccg cacatgacat gatgaccgcg
     3541 atgttcgagg tcgattcccg ctaccgcatc atctgggaac tgggcttcgc catcaacaca
     3601 cactcgacca tcgtgccagg caatttcgcg atgaacgagg tcttcggcgc ggagaatggc
     3661 acgatccact tcggcctcgg cttgaccccc cacacccagt accacctcga cctcatctgc
     3721 cctggcctca cggtactcac cgacaagggc caggtcgtgc tcggccgccg cgggcccgtc
     3781 ggcgccatgc cacgtgtgcg cgcagagggt tgtgcgtgcc tggagtgatg ctccagagcc
     3841 ggacgggact ggcgcgctcg atctccgtcg ggctcgcgct gtttccggtg ggactgttgt
     3901 tcgggttgac cgccgcacaa aatggctggc ggccaatgga agtcttcgcg ctcagcgcga
     3961 tcggcttcac ggggagcggc cagttcgccc tgctggcgct gaccaaggag ggagttgctc
     4021 ccctgctgat cttcttgatc atcctgctga tcaacctgcg ctatgtgccc atggcgttca
     4081 ccgccgccag aggcatggac ggggccgtgg gcaagcgggc gacgttggct cacttcgtca
     4141 gcgacgagtc ctatgcggtc gagcagatcg ccgacccgcc cgccgtgcgc gcgtcgatcc
     4201 ggctctggat cgccctcttc tgggtcgcgt ccactaccgc cggggccctc gtcgccatgt
     4261 tgctgccggc gcattgggcg ggctccatcg gcgcggtcac ctttccagcc aacgcgttgc
     4321 tcatcctgct ggcgctgctg cgcgtgcgag ccttcgtgga gcgcctgggc cggcgcggcc
     4381 tggcggcgat cctggcaagt ctccccatcg cgctcggcat ccggatcctt ctgggcagca
     4441 agctgttctg gctgcccagc atcctcctgg tcgggctcac catccattgg atctgcaaag
     4501 cgagggacgc gtcgtgacga ccacggcctg gatcggtatc accctcttgc tgggtgtcag
     4561 cctggtcgtc cgcgtctttc cggccttgct gcgcccgccc ctctcccagg ccacgctgga
     4621 gcagatcgag accatcctgc ccattgccgt gctgatcaac ctggccatct tctccgcgag
     4681 cagcgagata gcggcggcgc ctggcccggc gctgctgggg ctggggatcc tgggcgtgtt
     4741 gacgttcgca gcgcgtaccg ttcccatcct ggtgacgctg atcgcgtcca ccaccgcttt
     4801 cctcctgctc ctcacggcgc tgaatcgctg aggcctgggg catcggcacc gcacagctcc
     4861 aggaggacca gggagctcgc ctccgccggg ccatccgtca catcagatcg cagccgtgtc
     4921 catccagttg gccccgggat gcgctgggcc gatcccgcga atggaccacg ctgacggatg
     4981 gtgtctgtat gcaagaggca atgctcatcg aagatggggt tgacggccgg gacactccgg
     5041 gagtggtgga cgaggttccc gccggagtgc tcgagaagct gaaggagtgg gtgaagacgt
     5101 tcgacccttc cgcccgactc cagcttccac ggcacctggt ccacaagagc gatccacgca
     5161 acgtcttcat cgcccggctc gagcgcgtct cggaggcacg gccagacgaa ctcgcggcgc
     5221 agctcatctt cgacccaacc catccgtacc acttcgagca cccgcaggac cacgtcccgg
     5281 gaatgatgct catggaggca ggtcggcaac tcgggctggc ggtgacgcac ctgttctacg
     5341 gggtagcgct ggacgccatg ttcgtgctca acgacgtgaa ggcccgcttc cgccgcttcg
     5401 cggagctggg cgagcccgtc ttcgtgtact cggccgtgca cgagaaggag taccggcggg
     5461 ggcgcctcat ctcgatgcgc caggcggggc tcttcatcca gcggggcgag ttcctcggct
     5521 cgatggaggg aagctggagc gtgtacgacc ggcggctcat ggagcggctc cggcgccgac
     5581 cgcggccggg ggatggatcg gccacttccc gcccgatgac ggcccctgcc acggagtgat
     5641 gcccatgacg aaacactatg tggtggtggg gggcggtgtg tccggcatcg ccgcggcgca
     5701 ctacctccgg gaggcggggg ccgaggtgga gctcgtcgag caggaggaca cgctgggcgg
     5761 gcgcgtggcg ccagcgttgc tcgacggcca gcccatcgag ctggggggca agaacatcgg
     5821 ccgacgctac ctcctcttcc gcgagttcgc gcggaagatg gggcagacgg acttcgagcc
     5881 tttcggcatc aactcctcgc gcgtgcggcc cgacggccgg ctgatgaccg tggacagctc
     5941 gcaccgatgg cgcggcgtgc gggaactgat gcgggcctgt ccttcctcgg acctgctccg
     6001 cttcgccacg ctctgcgcgc gcgcgatctc ccgcccggag aacggctacc tgggctccgt
     6061 ccgcttcgat gccctcggag agcaccggga tgaccgcccg ctgagcgcgt acttcagcca
     6121 ggccttcagc gaggccgtgc tccgccccat ggtggtccgg atgaatggcg ccgagccgga
     6181 cgaggtcttc cccggcaacg tcggcaccaa cctccggatg ctcctggaca cgtacgagca
     6241 gccgaagcgc ggcatgcggg ccctcttcga tcgcttcgcg aggaccgtgc gggtgacgcg
     6301 aggcactcgc gcccgagggt tggtggtgcg gggcgggcgg gtagcggccc tggaactgga
     6361 ggggcccggg ggcgcccgcg aggagcgcac gtgtgatggg gtgctgctcg ccctgcccgc
     6421 gcaccccagc gccgcgctgc tggctccgca cgcgccgggt ctggccctgg cgctcggcca
     6481 cgtgcgctac ttcccggtgc tggtggtggt ggcccggtac gcgcggaagg tcttctcccc
     6541 ctccacccgc gcgctcgtct tcggcccgga gcacccgctg agcaacgcgg gggcgtacgg
     6601 catcaacgcc ctggacgtgg tgcgctacac gttcagcggg cgcaccgcac gccgggccct
     6661 ggcggccggg acggacgtgg aggcgctgct gcgcgtggga gagaaactgc tcggccacca
     6721 cttccccgtc caccccgccg agcgggtggc ctccgtcgcg cggcgcttcg agccgggact
     6781 gtgcgcctac acgccccacc atgcccgcct gcggcgcgag ctgaacgctg gcctggggag
     6841 gctccacggc ctccagctcg ccggggacta cctgcggggc gcctccatcg aggcctgctt
     6901 ccgcgcggcg aaggaatccg cggggcggat gctgaggaac cgatgagcgg cgctgactcg
     6961 ccacctgacg agtgcaagca aaggagagaa cggatgcgga aatactatct ggggccggag
     7021 gccagccatg aagtgccgtt caagaggctc ctggcgatct cccactacat catgcccaac
     7081 ttcggcactc tggagtggga tcgggtctcg cgcgagcagg tggacaagaa cctgcgctcg
     7141 ctgcaggacc cgggaggggc gctgaacgac gagctgatga tgtacatcca cgtgccgtac
     7201 tgccagtcgt tctgccatta ctgcaacttc aacaagaacc actatccctg gcaggacgag
     7261 gagcggctcc agcgctacac ggactacctc atcaaggaga tcgactacta cctgtcgctc
     7321 ccctacgtgc aggcgcggag attcacggcc atctacatcg ggggaggaag cccttccacg
     7381 ctcccggtct cggcagtgga gcggctgttc gcgcacctcg cgaaggtagc gccggggttc
     7441 gacaccctcg agaagacgtt cacgggcgag ccgcgcacgc tgcgcaagcc ggagctgctg
     7501 aaggtactcc acgactacgg cttcgaccgc gtgacgttcg gcatcgagac gctcaacccg
     7561 gagatccaca agaagatagg ccggtgggac tctccgagcg acgtggacgc ggtgttcgag
     7621 gggctggaga agctggggta cgagggcgac cgctgcgtcg acctcatgta tgacctgccg
     7681 gggcaatcgc tggtgggctt ccaggaggag ctggcgacac tggtggagca ctaccgaccg
     7741 gacgagatcg acgcgttcgg gaccgtgtac ctgccgtacc ggccgctgca caagctcatc
     7801 ctcgacggac gggtgccaca acctgggagc atctggcagc tcctgcggat gcgcgagcac
     7861 ctgtatgact acctgctgga gaacgactac cacaacacca tcgccgagac gtactcgcgc
     7921 aagccccagc gcagcatgta ccagacggcg cactgcgcgc ggcaggacat catcggcatc
     7981 ggctgcgcgg cacgcagcaa catcaaggac atggtgtcca tcaacccgga caaggtcgat
     8041 gtctggatga acaacatcga tcagtacggg gtgtccacgc agacactgca gagcatcggg
     8101 cggagcggag tgctggaccg gatcatggtg atgttcccgc gctacaagga actctccaag
     8161 gagttgctcg accgctactc ggacgtggag cacttcgagc aggtggagga cgtcctgcgc
     8221 tcgcatctcg aggtggggtg cgtggaggag caggagggcc ggtacgtcgt caacaagctg
     8281 ggcgtcatct ggcacggcaa cctgcagacg gactacatgg agcgttcgct gaacctgcag
     8341 ggaaaggtcc tcctgaaggt catctcggag aaggagagcc acttcgatcg agaggagcgc
     8401 ttcaaggtga acaaggcgac ccggttcatc gcgaagcaca tcgacaagta cccgaagctg
     8461 atgaagtagc ccgggaaggg ggcacccgat ggtcgacgtg gtgcgcaggc gcgagctcgt
     8521 accggtggag gatggtacgc ggctggaact ggaggtcttc gagcaggtgg aggaaaggaa
     8581 ggcaccggtt ctcctgtgcc tgccagcgat gggagtcccg gcgaggtact acgagccgtt
     8641 cgcgctcgag ttgcaccggc ggggtttcca cgtcgtcacg agcgacctgc gcgggcacgg
     8701 ggcgagctcc gtacgagtgg gccgggggac ggacttcggc tactacgaga tggtggcgcg
     8761 agacctgaag gcggtggtgc gggcggtgcg cgctgccttc caggacagcc cgctgttcct
     8821 gctggggcac agcctgggtg ggcagctcgg ctcgctctac ctgggcctgg agggtgaggg
     8881 aatacgaggg ctcgtcctcg tggcggcctc ttcggtgtac taccggaact acggggcgct
     8941 gggcggtgtg aaggtgctgc tcggcacgca gctcgcggcg gtcatcgcca cggtgtgggg
     9001 gtacttcccg gggcggaggc tacggttcgc ggacaacgag tcgacccggg tgatgcggga
     9061 ctgggcgcgc caggcgagga gggggcgcta cgtgctctcc ggggccgcgc aggacttcga
     9121 ggcgctgctc gcccgggtgg agcggccggt gctggccatc tcggtggagg gggattcgct
     9181 ggcgccaccg gccgcggtgg agcacctgta cgggaagatg cctcgggccc gggtggtgcg
     9241 gtggcacttc acgcggtccg atgccggggg agcgcacctg gatcacttcc gctgggtacg
     9301 ccacggtgct tccgtggcgg cgcgcatcgc gggatgggtg ggagaggtgc tcgcgggaga
     9361 gataggctcc ggccaagacc cgcaaagagg tgaaacatga gcgcagtccg tctgggccga
     9421 cgttcgtcat cttcgattct cttcctatcg ctctctgtcg tgggcatgct cgctggctgc
     9481 agttcgccgt cggagggccc gtctgacggg actcctccac ccgatgaagg gactccgcct
     9541 tcgttcacct ggggcaccgg cacggacacc ggcacgctcg cgggcgacgt gtggactcct
     9601 gggcgtgacg cgacgggact cgtgaacgcg gcgtcgtggg cgctcgtccc caaggggcgg
     9661 tggatcgagg tcgccggaac accgctcacc tcgctggacg cggaggtcaa ggcggcgctt
     9721 cctggcttcc gggatctcgg ctccgggggg atcgccgcgg tcatcaacgc ctactccggc
     9781 gtggcggtcg acgcgcctcg cgcccgctgg tgggccttcg gcggcggaca cgccgacagc
     9841 tccaacaacg gcctctaccg cttcgacatg gcgaagatgc gctggagcat cgagcagctc
     9901 cccgacaacc cagcgaactg gacgaaggtc gtctatggga acacgtacag ctcctacccg
     9961 cccgccgggg agtactccaa ggcccacccg gacagcgacg tctactcgga tgagttcttc
    10021 gatccggacc ggcccgccgc gtcgaccggc cacccggtgg cccgccacac gtacaacggg
    10081 ctcgtctaca acccggacct ccaggaagtg gcctttggcg tccggcggat gtggcgctac
    10141 agcctgacca cccacacctg gaagcgccac aaccccttca acaccccggg ggccacctac
    10201 gacggcgcga cgggatacgg cggcgcggcc ggctggacgt tctgggacga ggtgaaacac
    10261 cgctacctct ttggtcccac ggagaactac aactattcga ggtggtggtc cttcgacacc
    10321 gacgaccagt cctggaactg ggaggtcatc acgccccccg cgtggcattt cgcccagatg
    10381 gccagggtcg agcgcaagct ggtgagcttc ccctacccgg actcgcaaca ccagaccttc
    10441 cctccgcgac tggtcacggt cgacatggac acgggcaagt gggagttcct ggagctggag
    10501 cacgacctcg agctgtcacg cgtctacacc caggacttcg aggggatgat cttcacctac
    10561 gtgccaccgc tcaatcgcta catcgccgcg ttcccgtacg accgcgacgg cgatgggcgc
    10621 ttcgagtacc ggaccttcg
//
