LOCUS       NZ_CP022098            11647 bp    DNA     circular CON 17-FEB-2024
DEFINITION  Cystobacter fuscus strain DSM 52655 chromosome, complete genome.
ACCESSION   NZ_CP022098
VERSION     NZ_CP022098.1
KEYWORDS    RefSeq.
SOURCE      Cystobacter fuscus
  ORGANISM  Cystobacter fuscus
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Archangiaceae; Cystobacter.
REFERENCE   1  (bases 1 to 12349744)
  AUTHORS   Rupp,O., Goesmann,A. and Sogaard-Andersen,L.
  TITLE     Sequencing and comparative analysis of myxobacterial genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12349744)
  AUTHORS   Bruckskotten,M., Treuner-Lange,A., Rupp,O., Goesmann,A. and
            Sogaard-Andersen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUN-2017) Department of Ecophysiology, Max Planck
            Institute for Terrestrial Microbiology, Karl von Frisch Str. 10,
            Marburg, Hessen 35043, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2016
            Assembly Method                   :: Celera Assembler v. 8.1
            Expected Final Version            :: yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 10:04:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,093
            CDSs (total)                      :: 10,016
            Genes (coding)                    :: 9,906
            CDSs (with protein)               :: 9,906
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 26 of 110
            Pseudo Genes (incomplete)         :: 101 of 110
            Pseudo Genes (internal stop)      :: 1 of 110
            Pseudo Genes (multiple problems)  :: 17 of 110
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:24:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4696070
            Orig. end                         :: 4707717
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11647
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="22"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11647
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11647
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6647](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6647
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            364..2175
                     /locus_tag="CYFUS_RS19365"
                     /old_locus_tag="CYFUS_003824"
     CDS             364..2175
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1149: LuxR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /locus_tag="CYFUS_RS19365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_003824"
                     /product="AAA family ATPase"
                     /protein_id="WP_095986570.1"
                     /transl_table=11
                     /translation="MGETRIELLGGARILEERHKSRPLERKTAAFLAYLALEGETARGT
                     LAGLLWPESREATARNNLSQLLRRLGELLGEAGVEGRKTVRLRDDLRVDVRELVRGQLS
                     WLESPGDELLAGLNYDDCEALEDWLRATRPRVTGLQRKALEEQLWLEEREGRLNLALEA
                     AQRMLALEPTSEETFRHLMRLHHRMGNRDAALRVWRQCEEVLARELGIRPSAETSRLAG
                     ELERQPSEQPSVGSERKVLPLTVVHPPVLAGREREWAKLEAAHAARRPTFVLGPAGVGK
                     SRLIGDFARSRGRSLLLTSRPGDINVPFSTHARGVRTLLRHNPGLVLEPWVRRELSRLV
                     PELGPEPLPPPGTPEETARLFAAVLHMARQALRELDVLVFDDAQYSDSGSSELSFYLLA
                     QLEEDMLARRFPWILLGERTDETLWKGERIHMQVEAGLAELIQLEPLALPEVRTLLRGM
                     NEPRLEQMAEDISRYTGGNPLFIVETARHLIESNSLGGRFPEGLPPPGRARYIIQTRLE
                     GLSSEALRLAQVLAVARADVGLELAAEVLEVPVARLVEAWRELEEAALVRGAWFSHNLV
                     GEAVLSGLPASVHTLLATRLGRAGPVP"
     gene            complement(2242..3549)
                     /locus_tag="CYFUS_RS19370"
                     /old_locus_tag="CYFUS_003825"
     CDS             complement(2242..3549)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615639.1"
                     /locus_tag="CYFUS_RS19370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_003825"
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="WP_095986571.1"
                     /transl_table=11
                     /translation="MKKQWLMGLLLAAAPGVASAEVLVSGIVDVLVDGGNTYDWSKVEL
                     PGTQCGNGSQYKFFVHRSTTGSQNLMFFFEGGGACWDYDTCSGRAGVIGAANPNGITDD
                     YMTQFTAKYVSPIVNGADPGLPFRSRTDIATKDWNIVYLPYCTGDVHVGNNVATYADQT
                     GAQPPLSWHHSGYTNSLAAINYAKTQFPQVQKLLVTGFSAGGTATSASYYFVRRIINPA
                     RGYFLNDSGPIYLAPNVNDNSRPLHDKIRQSWNLDSVFNQFPASFDRNNLGTINRMLAM
                     EFPNDQLAYTGYSRDYNYSRFSYERFYTPNDQEAVLTRWKADQDKLVAELSLYNNYSYF
                     IPYERQINASHCSTIITFVGAHACQRMEKKKWYEYVNEPWQDYKCNSEMVSMTTFLQRF
                     INDNQRVRIYEPANGYNADDPGMSVLAPLINGALGG"
     gene            3747..4379
                     /locus_tag="CYFUS_RS19375"
                     /old_locus_tag="CYFUS_003826"
     CDS             3747..4379
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394936.1"
                     /locus_tag="CYFUS_RS19375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_003826"
                     /product="hypothetical protein"
                     /protein_id="WP_232537657.1"
                     /transl_table=11
                     /translation="MAILWWMPREDNPSSEAPGREERSRPPPPAVVPVAPTPSRPVAPQ
                     ATVAGEPDAGSSAPAPEPEQVPALEAAREHPVNLEKLREQMPDNLYWELGAPTKDPEIL
                     HRREEQARRWNALLGKVQSNTATEEEIHQYYDHRRAVSEDFIAFASRVLQEHGAQLSEQ
                     ERGLYALSIQMHRTRLDEIPRQVEDALARKATQDQRREEWQRGKKGP"
     gene            complement(4404..4892)
                     /locus_tag="CYFUS_RS19380"
                     /old_locus_tag="CYFUS_003827"
     CDS             complement(4404..4892)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CYFUS_RS19380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CYFUS_003827"
                     /product="hypothetical protein"
                     /protein_id="WP_157758531.1"
                     /transl_table=11
                     /translation="MRPNVQYRNSAAMRPPPVMHDTKTWTTSNGEMRLWRPAPHVIVTK
                     FKGTMYDAHLANLAMRAIEDIVASEPRVDIFHDWEDMELYATPARTLMTERAVPLAPNI
                     NTLSVLFGSKVVLMGVSLASIKLGGIPTFTQRKEFEQAVQQAAKSPPGTFKLKLPMEE"
     gene            5001..6647
                     /locus_tag="CYFUS_RS19385"
                     /old_locus_tag="CYFUS_003828"
     CDS             5001..6647
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626180.1"
                     /locus_tag="CYFUS_RS19385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_003828"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_095986574.1"
                     /sec_met_domain="DUF692 (E-value: 2.6e-23, bitscore: 81.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDAKASAWNLPWRGLGLSSNLDAADAPHPYRLLAEAPGLFDFVE
                     YSAPLSLEETRAAASLFPEMWERRGEVPVLFHPVHLNLYGPELEPERALAALDTHARAV
                     GSAWVGNDVGWWHSGGQPFPGYLYFTPPLTEAGLRDCAAHVLHVQSHLSMPLALENPAV
                     FARRGELHVLDFMARLHARTGLPLLLDLGHLLSYQLAAGLPLDAGLADFPLDQVIELHL
                     AGGVVTRRGGRSHYVDDHTQPVREELFGLLELLLPRCPSLRAVTFEGDGHPLDVALLTL
                     RRLRQLVPRAPRPALSLTPVERPAPPLVRESAPWTLFESGYGVTPEPGGDEEGARAEQD
                     FRFAVVAEVLDRDWPLSRLLLAGTRERLGAFCASREFRELFEGSGRSPGHAFAAWTRRR
                     LREHPDEGVAAAVSFETFLPHAARIRPRAAPGPGEVGLAEDVGLGGFPVDLTELVFAAR
                     ALRRHLTGRAWASEALEVSGLDALAQVARRPAPGPWRFIVRRKGRGLEVLTAATLVLDE
                     LAALERAPRPAREVPIQRLADAGVLGLLRWG"
     gene            complement(6654..7544)
                     /locus_tag="CYFUS_RS19390"
                     /old_locus_tag="CYFUS_003829"
     CDS             complement(6654..7544)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626179.1"
                     /locus_tag="CYFUS_RS19390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_003829"
                     /product="hypothetical protein"
                     /protein_id="WP_095986575.1"
                     /transl_table=11
                     /translation="MSLWLAGVAGGCHPVGEGSEQQDRSVQIWTVGSINGLSLNGLSLN
                     GLSLNGLSLNGLSLNGLSTSQFDLWFQTNPTLNDSVMRYVVLCAVPAGVTRTYTSALTG
                     STWSWTGGLGLAPDWAQGTPATLTEQRLVSACLAAHTNKFGLQIPLSVQGQGAAGTTVP
                     TDPGELEDYPEREGCFFGNLFNNEGIYAGNNRKALNHKESTPRACALSIKKSGPPECPP
                     IIRVDPDCSEICTQDAASGIYTSCTYNGITYPALTTRLRKQDIYTCGDGVCQVSEKCGT
                     KNDYDNCASDCGRCP"
     gene            7886..8521
                     /gene="udk"
                     /locus_tag="CYFUS_RS19395"
                     /old_locus_tag="CYFUS_003830"
     CDS             7886..8521
                     /EC_number="2.7.1.48"
                     /GO_function="GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="udk"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626178.1"
                     /locus_tag="CYFUS_RS19395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_003830"
                     /product="uridine kinase"
                     /protein_id="WP_095986576.1"
                     /transl_table=11
                     /translation="MPSPLVVGIAGGTASGKTTVARKVREALADCRVAFIDQDSYYRDL
                     SDLPMAERREVNFDHPDAFDTELLVAHLAELKAGRAIQKPVYDFVTCTRQPQTVRVEPG
                     DMILLEGILVLHMKPLRDEMSVRIYVDTDDDLRILRRLERDIHERGQDFDHVVSQYLRH
                     VRPMHMGFVEPSKHHAHIIVPQGGNNDIAIGMIVGALRARLMNPAAPR"
     gene            complement(8529..9521)
                     /locus_tag="CYFUS_RS19400"
                     /old_locus_tag="CYFUS_003831"
     CDS             complement(8529..9521)
                     /NRPS_PKS="Domain: Abhydrolase_1 (31-308). E-value:
                     4.5e-13. Score: 41.3. Matches aSDomain:
                     nrpspksdomains_CYFUS_RS19400_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626177.1"
                     /locus_tag="CYFUS_RS19400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_003831"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_332468398.1"
                     /sec_met_domain="PF00561 (E-value: 2.6e-12, bitscore: 46.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPVALPLEEGGLLGAARIAWCAYGERSEDNVVVLLHDLPHSHHA
                     LRPAGQDAVPYSGWGSELLGEGRPLDTAALHVVVPNLLGSPFGSTSPVAVDPHARRPYG
                     AALPTVTVLDMARAVAALLRSMKVERVRAVVGVGLGGLVALRLAALFAERVGGVAVLGA
                     ARTLPESLRERLGLTRHLLRLDPDFHDGHYAPGAGPRRTLRKQALEYLRLVHGPGGEAP
                     DATQALEAEAQALADTFDANAWALLCTAYAGADLSECLAEVRAPVLLAAAANDLLAPPA
                     RVRDTYHLLSAAGIRAHYHELPEPCSHQGLLAGSRRLHGPLRDFVRRRG"
     aSDomain        complement(8598..9428)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CYFUS_RS19400_Abhydrolase_1.1"
                     /evalue="4.50E-13"
                     /label="CYFUS_RS19400_Abhydrolase_1.1"
                     /locus_tag="CYFUS_RS19400"
                     /protein_end="308"
                     /protein_start="31"
                     /score="41.3"
                     /tool="antismash"
                     /translation="VVVLLHDLPHSHHALRPAGQDAVPYSGWGSELLGEGRPLDTAALH
                     VVVPNLLGSPFGSTSPVAVDPHARRPYGAALPTVTVLDMARAVAALLRSMKVERVRAVV
                     GVGLGGLVALRLAALFAERVGGVAVLGAARTLPESLRERLGLTRHLLRLDPDFHDGHYA
                     PGAGPRRTLRKQALEYLRLVHGPGGEAPDATQALEAEAQALADTFDANAWALLCTAYAG
                     ADLSECLAEVRAPVLLAAAANDLLAPPARVRDTYHLLSAAGIRAHYHELPEPCSH"
     misc_feature    complement(9485..9494)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            9602..10507
                     /locus_tag="CYFUS_RS19405"
                     /old_locus_tag="CYFUS_003832"
     CDS             9602..10507
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626175.1"
                     /locus_tag="CYFUS_RS19405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_003832"
                     /product="hypothetical protein"
                     /protein_id="WP_095986578.1"
                     /transl_table=11
                     /translation="MKRYFGVIVLIVGIILAAVVTTRASSARALEAQRDADFARVQKDY
                     LERVGWLRVNPDEKAYRQEVSSFFKAYFTQVDAHHDRYKLGKTYDAYLAELEKRGDKDD
                     RVQDRKAFYEYTRQVFDQMREGKYEPLWTATDKGMRLDVVSADVVKVLDKPQVRLRLAL
                     WGAQREERSDGKVKKMVTSASFKTQWKLTDERGRLQGEMSAEDPSMKVDFPERFIAEFP
                     PQMVLGHYDMDLVPNEVKKMEISFQVSSRAASGGDATASYVWKLEVPSEWRLGAGEKWE
                     GAEVTERPEEEIDPAKAARK"
ORIGIN
        1 ctgtcgagga acgcgcccac ggccctgccc accagcacct cccaggcggg caatctcgtg
       61 gggatcgctc agctcgtgcg ctcgggggcc atgccctttc cctacttcga tgccgcgagc
      121 gcgagcgcga acgtggtgga ccgtggcttg aacgtggggc tgcctttctt tggctccgcg
      181 ccggacatgg gggccagtga gtacgggctg tccacgggca ccatcggcct gaagtagaag
      241 agtccacgta cgacagatgc cgcgacctcc ggggtccgtc ccgaagcagg gcgggccccg
      301 gtgtgtcgat cggccacggg aaggctgtga cttgccaggg gcgcgcgtct acactcgcgc
      361 cgcttgggcg agacgcgtat cgagcttctg ggtggtgccc ggattctgga ggagcgccac
      421 aaatcgaggc cgctggagcg gaagaccgcg gcattcctgg cctacctggc gctggaggga
      481 gagacggcgc gggggacgct cgcgggtctg ctctggccgg agtcacgcga ggccaccgcg
      541 cgcaacaacc tgtcccaatt gttgcgtcgc ctgggcgagc tgctgggtga agccggtgtg
      601 gagggccgca agacggtgcg gctgcgcgat gatctccggg tggacgtgcg cgagctcgtg
      661 cgtggccagc tctcgtggct cgagagtccg ggagacgagc tgctcgcggg tttgaattac
      721 gacgactgcg aggcgctgga ggattggctg cgtgccaccc gcccgcgggt gacgggtctg
      781 caacgcaagg ccttggaaga gcagctgtgg ctggaggagc gggaagggcg gctgaacctg
      841 gccctggaag ccgcccagcg gatgctcgcg ttggagccca cctccgagga gacgttccgc
      901 cacctcatgc ggctgcatca tcgaatgggc aaccgcgacg ccgcgctgcg cgtctggcgc
      961 cagtgcgagg aggtgctggc gcgcgagctg ggcatccggc cctcggcgga gacgagccgg
     1021 ctggcgggcg agctggagcg acagccctcg gagcaaccct cggtgggctc ggagcgcaag
     1081 gtgcttccgc tcaccgtggt gcatcccccc gtgctggcgg gccgagagcg agagtgggcg
     1141 aagctggagg cggcccatgc ggcgcgccgg cccaccttcg tcctgggccc cgcgggcgtg
     1201 ggcaagtccc ggctcatcgg ggacttcgcc cgctcgcggg gccgctcgct gctgctgacg
     1261 tcgcgcccgg gtgacatcaa cgtgcccttc tccacgcatg cccggggggt gcgcaccctc
     1321 ctgcggcaca acccggggct ggtgctggag ccctgggtcc gtcgtgagct gtctcgcctg
     1381 gtgccagagc tgggtcccga gcccctgccc ccgccgggta ctccggagga gacggcccgc
     1441 ttgttcgccg ccgtgctcca catggcgcgc caggcgctca gggaactgga cgtcctcgtc
     1501 ttcgacgatg cccagtactc cgacagcggc agcagtgagc tcagcttcta tctcctcgcc
     1561 cagctcgagg aggacatgct ggcccggcgc tttccgtgga tcctcctcgg cgagcgcacg
     1621 gacgagaccc tctggaaggg cgagcggatc cacatgcagg tggaggcggg actcgcggag
     1681 ctcatccagc tcgagcccct ggcgctgccg gaggtgcgca ccctgctgcg tggcatgaac
     1741 gagccccggt tggagcagat ggccgaggac atctcccgct acaccggtgg caatcccctg
     1801 ttcatcgtgg aaacggcccg ccatctcatc gagtccaaca gcctcggggg acggtttccc
     1861 gagggactgc cgcctccggg ccgggcccgc tacatcatcc agacgcgtct ggagggtctg
     1921 tcctccgagg cactccggct ggcgcaggtg ttggcggtgg cccgcgccga tgtcggcctc
     1981 gagctggcgg ccgaggtgtt ggaggtgccc gtggcgcggc tggtggaggc ctggcgggag
     2041 ctggaggagg cggccctggt gcgcggcgcg tggttcagcc acaacctggt gggcgaggcg
     2101 gtgctgagcg ggctgcccgc ctccgtgcac acgctgctcg ccacgcgcct gggtcgcgcc
     2161 gggccagtcc catgaagaag gggagccctc ccggtccacg cggaaggctc cccttcgaga
     2221 cccggacggg ccggggacgc actagccgcc gagggcgccg ttgatgagcg gcgcgagcac
     2281 cgacatgccc gggtcgtcgg cgttgtagcc gttggcgggc tcgtagatgc gcacgcgctg
     2341 gttgtcattg atgaagcgct ggaggaaggt ggtcatggac accatctcgc tgttgcactt
     2401 gtagtcctgc cacggctcgt tgacgtactc gtaccacttc ttcttctcca tccgctggca
     2461 ggcgtgcgcg cccacgaagg tgatgatcgt gctgcagtgg ctcgcgttga tctgccgctc
     2521 gtaggggatg aagtagctgt agttgttgta gaggctcagc tcggcgacga gcttgtcctg
     2581 gtccgccttc cagcgggtga gcaccgcctc ctgatcgttg ggcgtgtaga agcgctcgta
     2641 ggagaagcgc gagtagttgt agtcccgcga gtagcccgtg taggcgagct gatcattcgg
     2701 gaactccatg gccagcatgc ggttgatggt gcccaggttg ttgcgatcga aggacgcggg
     2761 gaactggttg aacaccgagt ccaggttcca cgactggcgg atcttgtcgt gcagggggcg
     2821 cgagttgtcg ttgacgttgg gcgccaggta gatggggccg gagtcgttca ggaagtagcc
     2881 ccgggccgga ttgatgatgc ggcgcacgaa gtagtacgag gccgaggtgg cggtgccgcc
     2941 cgcgctgaac ccggtgacga gcagcttctg cacctggggg aactgggtct tggcgtagtt
     3001 gatggccgcg agcgagttgg tgtagccgga gtggtgccag gagagcggcg gctgcgctcc
     3061 cgtctggtcg gcgtaggtgg cgacgttgtt gcccacgtga acatcgcccg tgcagtaggg
     3121 caggtagacg atgttccagt ccttggtcgc gatgtccgtg cggctgcgga agggcaggcc
     3181 cggatcggcg ccgttgacga tgggcgagac gtacttcgcc gtgaactgcg tcatgtagtc
     3241 gtcggtgatg ccgttgggat tggccgcgcc aatgacaccc gcgcgcccgc tacacgtgtc
     3301 gtagtcccag cacgcgccgc caccttcgaa gaagaacatc aggttctgcg agcccgtggt
     3361 gctgcgatgg acgaagaact tgtactgcga gccgttgccg cactgggttc ccggcagctc
     3421 caccttgctc cagtcgtagg tgttgccgcc gtccaccagc acgtcgacga tgccagagac
     3481 cagcacctcg gcgctcgcga cgccaggtgc agcggccagg agaaggccca tcagccattg
     3541 ctttttcatg tgagtattcc tgtgggggct tggggtaagg ctatgcgatt cagttctcga
     3601 ccatccggaa agcacggaga accggggcgc actttatcgc gcggggttgc gaagcgcgag
     3661 tggcgcgagg tatctggacc ggtatgggtg tcacttcgcg gtcgaaggct ttgccatggc
     3721 tcggcgcgtc aatcattgtg gtgctgatgg ctatcctgtg gtggatgcca cgcgaggaca
     3781 acccgtcttc cgaggcaccc ggacgcgagg agcggagccg gcccccgcct ccggcggtgg
     3841 tccccgtggc gcccacgcca tcccgccctg ttgctcccca ggcaactgtc gcgggcgagc
     3901 ccgatgccgg gtcctccgca cccgcgcccg agcccgaaca ggtccccgcg ctcgaggcgg
     3961 cgcgcgagca tcccgtgaac ctggagaaac ttcgcgagca gatgcccgat aatctttatt
     4021 gggagctggg tgccccgacg aaggatccgg agatcctcca caggcgcgag gagcaggcgc
     4081 ggcgctggaa cgcgttgctg ggaaaggtgc agtcgaatac ggccacggag gaggagatcc
     4141 accagtatta tgatcaccgc cgcgcggtgt ccgaggactt catcgcgttc gccagccggg
     4201 tgctccagga gcatggcgct caactgtcgg agcaggagcg cggcttgtat gcgctgagca
     4261 tccagatgca ccgcacccgc ctggatgaga ttccgaggca ggtggaggac gcgctcgcgc
     4321 gcaaggcgac ccaggatcag cgccgggagg agtggcagcg cggcaagaag ggcccatgag
     4381 gcgggccctg gggcaggggg ggttcactcc tccatgggca gcttgagctt gaaggttccc
     4441 ggaggggact tcgcggcctg ctgcaccgcc tgctcgaact ccttgcgctg ggtgaacgtg
     4501 ggaatgccgc ccagcttgat gctggccagc gacacgccca tgagcaccac cttggagccg
     4561 aacaggacgc tcagggtgtt gatgttcggg gcgaggggca cggcccgctc cgtcatcagg
     4621 gtccgtgccg gggtggcgta cagctccatg tcttcccagt catggaagat gtccacccgg
     4681 ggctcggagg cgacgatgtc ctcgatggcg cgcatggcga ggttcgccag gtgcgcgtcg
     4741 tacatcgttc ccttgaactt cgtgacgatg acatgggggg cgggccgcca cagccgcatc
     4801 tccccattcg acgtggtcca tgtcttggtg tcatgcatga caggaggagg gcgcatggcg
     4861 gccgagttac ggtactgcac gttcggacgc aagaggtgtc cctggatccg ggtgcaccgg
     4921 ccaaccccgt gcccggggat tccagtactc gtgcccttga acggggagaa tgaaacgggc
     4981 tcgcgttagg gtgggtaccc atgagcgacg cgaaggcatc cgcctggaac ctgccctgga
     5041 ggggcctggg cttgagcagc aacctcgacg cggcggacgc accgcacccc taccgcctgc
     5101 tcgccgaggc gccgggcctc ttcgacttcg tggagtacag cgcgccgctg tcgctcgagg
     5161 agacgcgggc cgcggcctcg ctcttcccgg agatgtggga gcgccgggga gaggtgccgg
     5221 tgctcttcca tccggtgcac ctcaacctct atggccccga actggagccg gagcgggcgc
     5281 tcgccgccct ggacacgcac gcgcgggcgg tgggcagcgc gtgggtgggc aacgacgtgg
     5341 ggtggtggca ctcggggggg cagccctttc cgggctacct ctacttcacg ccccccctca
     5401 ccgaggcggg cctgcgcgac tgcgccgcgc acgtgctcca cgtccagtcc cacctgtcca
     5461 tgccgctcgc gctggaaaat cccgctgtct tcgcccggcg gggtgagctg cacgtgctgg
     5521 acttcatggc ccgcctgcac gcgcgcaccg ggctgccgct gctgctcgac ctggggcacc
     5581 tgctcagcta ccagctcgcc gccgggctgc ccctggacgc ggggctcgcg gacttcccgt
     5641 tggatcaggt catcgagctg cacctcgcgg ggggcgtcgt cacccggagg ggcggccgga
     5701 gccactacgt ggatgaccac acccaaccgg tgcgcgagga gctcttcggc ctgctggagc
     5761 tgctcctgcc gcgctgcccc tcgttgcgcg ccgtcacctt cgagggagat ggacacccgc
     5821 tcgacgtggc gctcctcacg ctgcgccgcc tgcgccagct cgtgccccgt gcgccgcggc
     5881 ccgcgctgtc cctcaccccg gtggagcggc ccgcgccgcc tcttgtccgg gagagcgcgc
     5941 cctggacgct cttcgagtcc gggtatggcg tgacgccgga gccgggcggc gacgaggagg
     6001 gagcccgggc ggagcaggac ttccggttcg cggtggtggc cgaggtgctc gatcgcgact
     6061 ggcccctgtc gcggttgctg ctcgcgggca cgcgtgagcg cctgggggcg ttctgcgcgt
     6121 cgcgcgagtt ccgcgagctc ttcgagggtt cggggcgctc gcccgggcat gcgttcgcgg
     6181 cctggacgcg gcggcgtttg cgcgagcatc cggacgaggg ggtcgcggcg gcggtctcct
     6241 tcgagacgtt ccttccccac gccgcgcgca tcaggccccg cgccgcgccg ggaccgggcg
     6301 aggtggggct ggccgaggac gtgggcctgg gcggcttccc ggtggatctc accgagctgg
     6361 tcttcgccgc ccgggccctg cgccgtcacc tgacggggcg ggcctgggcg agcgaggccc
     6421 tggaggtgag cgggctggac gcgctcgcgc aggtggcacg gcgtccggcg cccggtccgt
     6481 ggcgcttcat cgtgcggcgc aagggccggg ggctggaggt gctgacggcg gcgaccttgg
     6541 tgctcgatga gctggccgcg ctggagcggg cgcctcggcc cgcgcgcgag gtgcccatcc
     6601 aacggctcgc cgacgcgggg gtgctcggcc tgctgcgctg gggatgaagg ggctcagggg
     6661 cagcggccac agtccgacgc gcagttgtcg tagtcattct tggtgccgca cttctcgctc
     6721 acctgacaga cgccgtctcc acacgtgtag atgtcctgct tgcgcagacg cgtggtgagg
     6781 gccgggtagg tgatgccatt gtaggtgcag gacgtgtaga tgccgctcgc ggcgtcctgg
     6841 gtgcagatct ccgagcagtc cggatcgacc cggatgatgg gcgggcactc cggaggtccg
     6901 gacttcttga tggagagggc gcaggcgcgc ggcgtgctct ccttgtggtt gagcgccttg
     6961 cggttgttgc cggcgtagat gccctcgttg ttgaagaggt tgccgaagaa gcagccctcg
     7021 cgctccggat agtcctcgag ctcgccggga tcggtgggga cggtcgtgcc cgcggcgcct
     7081 tgcccctgga cggacaaggg aatctgcaag ccgaacttgt tggtgtgggc ggccagacac
     7141 gcggagacga ggcgctgctc ggtgagcgtg gcgggcgtgc cctgggccca gtccggtgcg
     7201 aggcccaggc ctcccgtcca gctccaggtg ctccccgtga gggcggacgt ataggtgcgc
     7261 gtgactcccg ccggcacggc gcacagcacc acgtaccgca tcaccgagtc gttgagtgtg
     7321 ggattggtct gaaaccagag atcgaactgg ctggtggaca ggccattgag cgacaggcca
     7381 ttgagcgaca gtccgttgag tgacaagcca ttgagcgaca ggccattgag cgacaggcca
     7441 ttgatggagc ccacggtcca gatctggacg gaccgatcct gctgctcact cccttccccg
     7501 acggggtgac aaccacctgc cactcccgcc agccacagcg acatcgccgc tcggaaacac
     7561 agcccgtgga tagaggacag acgacgagac gtctcgttcg gtacgttttg catgtcatcc
     7621 ccccggcggc tagggacagc accggtctgc aacgagcact ggaccgcgtc catggagtga
     7681 agccacgctc atggctccac gcctgggtta tacgcctaat cagacaaact tgaaatacca
     7741 gccagtctgg cgaacctgga gtggcgtcgg tttgaacagg gagtccgtgg actacaggac
     7801 tgtgaggtgc cccggaggga gggggaaagg tggagttctg gacacagcct tgcgcccgtc
     7861 cgtccactgc ggtagttcgc gccccatgcc gtcacccctc gtcgtaggca tcgccggtgg
     7921 taccgcttcc ggcaagacga cggtcgcccg gaaggttcgc gaggcgctcg ccgactgccg
     7981 tgtggccttc atcgatcagg attcgtatta ccgggacctg tcggacctgc cgatggccga
     8041 gcgccgcgag gtcaacttcg accatccgga tgccttcgac acggaactgc tcgtggcgca
     8101 cctggcggag ctcaaggcgg gccgcgccat ccagaagccc gtgtacgact tcgtcacctg
     8161 cacgcggcag ccgcagacgg tgcgcgtgga gcctggggac atgatcctcc tggagggcat
     8221 cctcgtgctg cacatgaagc cgctgcgcga cgagatgagc gtgcgcatct acgtggacac
     8281 ggatgacgac ctgcgcatcc tgcgccggct cgagcgcgac atccacgagc gcggccagga
     8341 cttcgatcac gtggtcagcc agtacctgcg ccacgtgcgg cccatgcaca tgggcttcgt
     8401 cgagccctcc aagcaccacg cgcacatcat cgtgccgcag gggggcaaca acgacatcgc
     8461 catcgggatg atcgtcggcg cgctgcgcgc ccggctgatg aaccccgccg cgccgcgctg
     8521 agcgcgcttc agccgcggcg gcgcacgaag tcgcgcaggg ggccgtgcag ccgccgggag
     8581 ccggcgagca ggccctggtg cgagcagggc tcgggcagct cgtggtagtg cgcccggatg
     8641 cccgccgcgc tgagcaggtg gtacgtgtcg cgcacgcgcg cgggaggggc cagcaagtcg
     8701 ttcgcggccg ccgcgagcag caccggggcg cgcacctcgg ccaggcactc ggagaggtcc
     8761 gcgcccgcgt aggccgtgca cagcaatgcc caggcgttcg cgtcgaaggt atccgcgagg
     8821 gcctgggcct cggcctccag cgcctgggtg gcgtccgggg cctctccgcc gggtccatgg
     8881 acgagccgca ggtactccag ggcctgcttg cgcagcgtgc gccggggccc ggctcccggg
     8941 gcgtagtgcc cgtcatggaa gtcgggatcc aggcgcaaca ggtggcgggt gaggcccagg
     9001 cgctcgcgca gggactcggg cagggtgcgc gccgcgccga gcaccgccac gccccccacc
     9061 cgctcggcga agagcgccgc gagccgcagt gccaccagcc cgccgagccc cacgcccacc
     9121 accgcgcgca cgcgctccac cttcatcgag cgcaagagcg ccgccaccgc gcgcgccatg
     9181 tccagcaccg tcaccgtggg cagcgcggcg ccatagggcc ggcgcgcgtg aggatccacc
     9241 gccaccggcg acgtggagcc gaacggactg ccgagcaggt tgggcaccac cacgtgcagc
     9301 gccgccgtgt ccaggggtcg cccctcgccg agtagctccg agccccaccc ggagtacggg
     9361 acggcgtcct gccccgcggg gcgaagcgcg tggtgggagt gcggcagatc gtgcaacagc
     9421 accacaacat tgtcctcgga gcgctcgccg taggcacacc aggcgatccg ggctgcgccc
     9481 agcaggccgc cttcctccag gggaagtgca accggagaca cgtaggatcc gtgggtctgg
     9541 tacacgagag ccaggcttca tatcactacg gccgtttttc ccgatcccag gagttgctcg
     9601 cgtgaagcgt tatttcggag tcatcgtgct catcgtcggc atcatcctcg ccgcggtggt
     9661 gacgacccgt gcctcgagtg cccgggcgct cgaggcccag cgggatgcgg acttcgcccg
     9721 ggtgcagaag gactatctgg agcgcgtggg ctggctgcgc gtcaacccgg acgagaaggc
     9781 ctaccggcag gaggtgtcgt ccttcttcaa ggcgtacttc acgcaggtgg acgcgcacca
     9841 cgatcgctac aagctgggca agacatacga cgcgtacctc gccgagctgg agaagcgcgg
     9901 cgacaaggat gaccgcgtcc aggatcgcaa ggccttctac gagtacacgc gccaggtgtt
     9961 cgatcagatg cgcgagggca agtacgagcc gctgtggacg gcgacggaca agggcatgcg
    10021 cctggacgtg gtgtccgcgg acgtggtgaa ggtgctcgac aagccgcagg tgcgcttgcg
    10081 gctggcgctc tggggcgccc agcgcgagga gcgctcggac ggcaaggtga agaagatggt
    10141 gacgagcgcc tcgttcaaga cgcagtggaa gctcacggac gagcgcggca ggttgcaggg
    10201 cgagatgagc gcggaggacc cctcgatgaa ggtggacttc cccgagcgct tcatcgccga
    10261 gttcccgccg cagatggtgc tgggccacta cgacatggac ctggtgccca acgaggtgaa
    10321 gaagatggag atctccttcc aggtgagctc gcgcgcggcg tcgggcgggg acgccacggc
    10381 ctcctacgtg tggaagctgg aggtgccctc ggagtggcgg ctcggcgcgg gcgagaagtg
    10441 ggagggcgcc gaggtcaccg agcgccccga ggaggagatc gatccggcca aggcggcgcg
    10501 caagtaggcg ctagccgccc accaccgtga agtgcgagcc caaccggtac gccgacagcg
    10561 tgccgagctc gtccaggaag tagaggttga gctgggcgtc ggcctggagc gccatgagtc
    10621 ccgcgccggc gcgcacctcc gcgagcacgt cgccgcggag gggatccacc gcgcgcacgc
    10681 gctcgcccgg caccagggcg acgccgcgcg agacgatggg ccgcagggga cgggtgagct
    10741 gctcgcccga ggcgcccacg cgccagtcca cctcgcccga gtcgctcacc cgcgccgccg
    10801 cgcccagggc ggaggtcacc agcaccgcgc ccggcaggcg cgcgagcgcg aagggccccg
    10861 gtcccaggtg cagcgcgcgc tgccagcgcg actgtcccgc cgcgtccacg cacaagagca
    10921 cgccctctcc ctccagctct ccggcgatgt acacgcgctt gccgctgggc agcggcgtgg
    10981 agggcagggc gagtgacatc tcgtacgtcc acaccgcctc gccggtgtgc gcgtccgcga
    11041 gcagcagcgc cgagttggag ccctggccga gcagcgccac gaagcgccgc gcccagggca
    11101 ccggcgcgcc caggaagggc agggacgcgc tcatgcggaa gcgcacctgg ccatccccga
    11161 gccccagccc gtagaggtag cccgagtccg tcgccagcag ggcgcggtgg gcatggatgg
    11221 acaggtagcc ccggcgcgtg cgcgcggggg tcagccgcca cgcctcgcgg cccgtcacct
    11281 cgttgagcgc gagcaccacc cgctcatccg ccagggtgta gagcatgccg tactgccgca
    11341 ggagcaacgg gcccacgtgc aacccgtcgt gcacgcgcaa ccagtccgct cccgagccct
    11401 tgccgggaaa gccgcacagg cgcaccgcgc tcgccgccag gctgtagccg tcctccgagg
    11461 ccgccacgcc atggctcgcc gcccgccgcc acacctgctt gcccgtcttg cgatcgaagg
    11521 cgcaggccat gtccggcgac gagtacacca gcccctggcg ccccatcatc agccgcccca
    11581 tgtccgcgtc caccagcttg cgctgctccc acaggctctc gaagcgcagc cggcgcagcc
    11641 gtcccgg
//
