LOCUS       NZ_CP022098            11173 bp    DNA     circular CON 17-FEB-2024
DEFINITION  Cystobacter fuscus strain DSM 52655 chromosome, complete genome.
ACCESSION   NZ_CP022098
VERSION     NZ_CP022098.1
KEYWORDS    RefSeq.
SOURCE      Cystobacter fuscus
  ORGANISM  Cystobacter fuscus
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Archangiaceae; Cystobacter.
REFERENCE   1  (bases 1 to 12349744)
  AUTHORS   Rupp,O., Goesmann,A. and Sogaard-Andersen,L.
  TITLE     Sequencing and comparative analysis of myxobacterial genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12349744)
  AUTHORS   Bruckskotten,M., Treuner-Lange,A., Rupp,O., Goesmann,A. and
            Sogaard-Andersen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUN-2017) Department of Ecophysiology, Max Planck
            Institute for Terrestrial Microbiology, Karl von Frisch Str. 10,
            Marburg, Hessen 35043, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2016
            Assembly Method                   :: Celera Assembler v. 8.1
            Expected Final Version            :: yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 10:04:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,093
            CDSs (total)                      :: 10,016
            Genes (coding)                    :: 9,906
            CDSs (with protein)               :: 9,906
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 26 of 110
            Pseudo Genes (incomplete)         :: 101 of 110
            Pseudo Genes (internal stop)      :: 1 of 110
            Pseudo Genes (multiple problems)  :: 17 of 110
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:24:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2314641
            Orig. end                         :: 2325814
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11173
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="10"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11173
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11173
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6173](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6173)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(2153..2168)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.59"
                     /tool="antismash"
     misc_feature    2652..2661
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    complement(2890..2903)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 21.45"
                     /tool="antismash"
     misc_feature    complement(2996..3014)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 20.83"
                     /tool="antismash"
     gene            3226..4005
                     /locus_tag="CYFUS_RS09620"
                     /old_locus_tag="CYFUS_001908"
     CDS             3226..4005
                     /NRPS_PKS="Domain: PKS_KR (7-172). E-value: 6.2e-19. Score:
                     60.5. Matches aSDomain:
                     nrpspksdomains_CYFUS_RS09620_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /locus_tag="CYFUS_RS09620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_001908"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_157758356.1"
                     /sec_met_domain="adh_short (E-value: 9.9e-37, bitscore:
                     125.8, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 3.2e-30, bitscore:
                     105.1, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRLENKVAVITGGGGGIGAATAKLFLQEGARVLIVGRTEEKLRK
                     TLQELHHENLGYAVADVSRVEDTRRYIQQAAERFGGVDVLVGNAGAEGPYKPLDEHSVE
                     DFDEVMAVNVRGCWLAVKYAFPEMRKRGGGSIVLTSSVMGLVAFPSNSAYTAAKHAVVG
                     LARSLAHDGAPFNIRVNAVCPGVIDNDMTAGIHTRLAPGNEDVVKSRMAQRIPMQRYGT
                     NEEIARLNLFLASDESSYSTGGVFVSDGGMTAGVRGR"
     aSDomain        3247..3741
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CYFUS_RS09620_PKS_KR.1"
                     /evalue="6.20E-19"
                     /label="CYFUS_RS09620_PKS_KR.1"
                     /locus_tag="CYFUS_RS09620"
                     /protein_end="172"
                     /protein_start="7"
                     /score="60.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAVITGGGGGIGAATAKLFLQEGARVLIVGRTEEKLRKTLQELHH
                     ENLGYAVADVSRVEDTRRYIQQAAERFGGVDVLVGNAGAEGPYKPLDEHSVEDFDEVMA
                     VNVRGCWLAVKYAFPEMRKRGGGSIVLTSSVMGLVAFPSNSAYTAAKHAVVGLARSLAH
                     DG"
     CDS_motif       3253..3336
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CYFUS_RS09620_0001"
                     /evalue="1.40E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="CYFUS_RS09620"
                     /protein_end="37"
                     /protein_start="9"
                     /score="21.0"
                     /tool="antismash"
                     /translation="VITGGGGGIGAATAKLFLQEGARVLIVG"
     CDS_motif       3613..3732
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_CYFUS_RS09620_0002"
                     /evalue="4.50E+00"
                     /label="PKSI-KR_m1"
                     /locus_tag="CYFUS_RS09620"
                     /protein_end="169"
                     /protein_start="129"
                     /score="0.1"
                     /tool="antismash"
                     /translation="KRGGGSIVLTSSVMGLVAFPSNSAYTAAKHAVVGLARSLA"
     gene            complement(4135..4776)
                     /locus_tag="CYFUS_RS09625"
                     /old_locus_tag="CYFUS_001909"
     CDS             complement(4135..4776)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025604.4"
                     /locus_tag="CYFUS_RS09625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_001909"
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="WP_095984949.1"
                     /transl_table=11
                     /translation="MHRPNIRKHRGFTIIEFTIVTAMLSILAVIAIPNFLKRQALAAQG
                     EAKGNLKAWFSAQRAYHEENGRYSENVHTVGFLPESGNRYAYYFGSALNCIIRNASGVT
                     DSPNANCITVDRAEFPNNVLTPKVTPPSSPTYEGEGANPGMPGLGGCTTGIGCNISGLA
                     AGNIDDEFRGIDSWWISTKDAQTITAACGNSETESVAGEPYNSFNDIDCD"
     gene            complement(5001..6173)
                     /locus_tag="CYFUS_RS09630"
                     /old_locus_tag="CYFUS_001910"
     CDS             complement(5001..6173)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /locus_tag="CYFUS_RS09630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_001910"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_095984950.1"
                     /sec_met_domain="DUF692 (E-value: 6.6e-26, bitscore: 90.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPPRVGLNLLTDDAFREAARPLFAEGLVTALEWDIDDSWGFSART
                     PPPHWTERMLDLYAEEGALYGHGVWLSVLTADWQPRQDAWLERLAQECRQRPYRHVSEH
                     FGFTAAGPFTRSTMLPLPYCAAAVDIGRDRLGRLRAATGGPVGLEVLANTLAPSDALHQ
                     GAFLDAILTPTGGFLVLDIHNVWTQAINTGLPPELLLETHPLECVREIHVSGGSWARAL
                     RAGDPRPVRLDSHDGPLLPPVLSLLRRALDLCPHCEVVIVERRSETLESRAVRDEWRAQ
                     YRAVVEVVTEASATPVSPVRDASTTPLPDPLDVEELALYQRELVTALVEESESRVILSR
                     LSQGIAGARLAPYLETFDPRMVELLAVLASRWSALDPAQDTRGGAHEERR"
     gene            complement(6192..6347)
                     /locus_tag="CYFUS_RS50485"
                     /old_locus_tag="CYFUS_001911"
     CDS             complement(6192..6347)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CYFUS_RS50485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CYFUS_001911"
                     /product="hypothetical protein"
                     /protein_id="WP_157758357.1"
                     /transl_table=11
                     /translation="MNQNLREQATQLLDMANNTKERNARPTPCPACGMMMALPESSTQR
                     VTDAGR"
     misc_feature    complement(6433..6447)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.58"
                     /tool="antismash"
     gene            complement(6632..6976)
                     /locus_tag="CYFUS_RS50490"
     CDS             complement(6632..6976)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CYFUS_RS50490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157758358.1"
                     /transl_table=11
                     /translation="MKPHTILESVVHWCSTPSESLATVSDHGQPDLDNLKLLLEGFMTG
                     ALQSEAGEQWHQFERWLGWRRPDLFREGLTWYGEGLLMECGGEHSRAAELLKTYVEEYQ
                     RSPPDSRGQT"
     gene            complement(7347..7880)
                     /locus_tag="CYFUS_RS09640"
                     /old_locus_tag="CYFUS_001913"
     CDS             complement(7347..7880)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CYFUS_RS09640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CYFUS_001913"
                     /product="hypothetical protein"
                     /protein_id="WP_095984952.1"
                     /transl_table=11
                     /translation="MNCPQEPKATLVCSSYHAAATNMFAALARGDMATAMYYLSALTGA
                     SVTQQQAIINGLTHFFDEGNQIAGQCGDVARGGFQVLHRLGEFMGSRPYFLKIISTEKR
                     LILGFELRTGVAKQLADNGVHIAVKFEGRVFDAYTGPGGMDEAEYLRRIHTYTGQEIAS
                     KEYASFPSLFRELE"
     misc_feature    complement(7969..7985)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.35"
                     /tool="antismash"
     gene            complement(8081..10165)
                     /locus_tag="CYFUS_RS09645"
                     /old_locus_tag="CYFUS_001914"
     CDS             complement(8081..10165)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1117:
                     sodium/hydrogen exchanger"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629658.1"
                     /locus_tag="CYFUS_RS09645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_001914"
                     /product="cation:proton antiporter"
                     /protein_id="WP_095984953.1"
                     /transl_table=11
                     /translation="MHDAHDFLKALATVLCVAAVTSVIFQRLRQPVVLGYIIAGLIMGP
                     HVPIPLVADPTIVQTLSELGVILLMFSLGLEFSLGKLLKVGFTAGLTAIIQCSLMIWLG
                     FVVGRSFGWTSRESIFTGAIIAISSTTIIAKAFDEQGIKGKLREIVVGILIVEDLIAIL
                     LMATLTAISTGAGLSAGQLTLTVGRLALFLVGLVVVGLMVIPRTVRAVHRLKRPETTLV
                     ASIGICFAIALLAQAFGYSVALGAFLAGSLVAESGEEQEIEHLVQPVRDMFAAIFFVSV
                     GMLIDPGLIAENWAAVAVLTGVVILGKLVGVALGVFLTGSGTRTAIQSGLSLAQIGEFS
                     FIIAGLGLSLNATGSFLYPVAVAVSAITTLTTPFLIRASGPVANYVDRKLPKPLQTFAA
                     LYGSWVEELRNAPPRQTAGTKIRSKVRLMLLDAALLAAIIIGTSLFLGRMSAALELRSG
                     LGATVARWLVIFLAAMLATPFCVGVVRLASSLGTLLAETAFPSVTGRLDRAAAPRRALV
                     VTLQLASVLGVGAPLVAITQPFLPRFLGAAVLLILLAVLGMGFWRSATNLQGHVRAGSQ
                     VIVEALASQSRGKPQAHHAESLDTLHAILPGLGEPTPVRLESGSAAVGRTLAELNLRGM
                     TGATVLAIRRGEDKVVVPTADEQLREGDVLALAGTHEAIDSARAVLSGSTPAEQQPASA
                     "
     gene            complement(10267..10428)
                     /locus_tag="CYFUS_RS50495"
                     /old_locus_tag="CYFUS_001915"
     CDS             complement(10267..10428)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CYFUS_RS50495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CYFUS_001915"
                     /product="hypothetical protein"
                     /protein_id="WP_157758359.1"
                     /transl_table=11
                     /translation="MREEQNPSASTPTPQPPSPAPVEYERWQTGRLIVAVLTCLFIIGA
                     AIAYEALG"
     gene            complement(10500..10955)
                     /locus_tag="CYFUS_RS09650"
                     /old_locus_tag="CYFUS_001916"
     CDS             complement(10500..10955)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CYFUS_RS09650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CYFUS_001916"
                     /product="hypothetical protein"
                     /protein_id="WP_157758360.1"
                     /transl_table=11
                     /translation="MKTSPRSPRREQGLARWMVLGGAACALLLAVLAGEPSSSVQRRSE
                     VHEAASTARPARSASVSATRRPQHERLVGDREAPLHGALELPRPAVKPLPGYKRFDSRH
                     GLAPRLETDVTPLTRMHRPCEPTRRHGRIADRPLIENCPAQGPPRLA"
ORIGIN
        1 gtcctggtca accccgaggg aatcaccgac ccgagcggtt cctatgcgac cgcgcatgag
       61 ccgtctttcg atgactcgtc gtggaggaag ctgaatgttc ctcacgactg gagcatcgag
      121 cgggacccca gcccggggcc ggggaccacc agtgggacgg gcttcttcca ggggggtctg
      181 ggctggtacc ggaagacttt caccctcccg tcgtcgatgg cgggaaagaa gctctccatc
      241 gaattcgatg gcgtgtacat ggactcctac gtctatgtga atggtgaact ggtgggcaac
      301 cacccctatg gctacaccgc cttcgacgtg gacctgtcct cccgggtgta cacggatggg
      361 gtgacgccca acgtcatcgc cgtgaaggtc cagaaccagc ttccgagcag ccggtggtac
      421 tccggcagtg gcatctacag gcacgtccac ctgacggtga cggaccccat ccacgtggtg
      481 cgttggggca ccttcgtcac cgtcccggaa ctcgagaaga cggtgggaga gggctacgcg
      541 aacgtccgcg tgcagaccga cgtggtcaac gagggcggca ccccgaagga cgtagaggtc
      601 cgctccctgg tgaaggacgc caacgggaag gtcgtggcgc aggtcacgtc actcgccacc
      661 gcgctgggcc cggcgcccca gcggctcacg gccaccctcc gggtcgagca ccccacgctc
      721 tggtcgttgg cgaatcccta ccggtacacc ctggaaaccg ccctcgtctc cgagggcaag
      781 gaggtggaca ccacctccac ccgcttcggc atgcggtatt tccgcatcga tcccaacgag
      841 ggcttctcgc tcaacggcga gtccacgaag atccagggag tcgatctgca ccatgatctc
      901 ggcgccctgg gggcggccgt tcaccgcgat gccctggtgc ggcagatgac gatcatgaag
      961 agcatggggg tcaacgccct caggaccgcg cacaacccgc ctgctcccga actgatcgac
     1021 atctgcgagg agatgggcat cgtcatgctg gtggaggcct tcgactcctg gaggaccggg
     1081 aagaacccgt atgactacgg gcgcttcttc gatgagcaca gtgatgccga catcaaggag
     1141 atggtgaacg ccgccaagaa ctctcccgcg gtcatcatgt ggtccattgg caacgagatt
     1201 ccggattcga cctcggaggc gggtctggcg atggcccagc gactggtcga ggacatcaag
     1261 tccatcgaca cctcccggcc catcgtcatc ggctcggaca agtaccggcg cgtgcctgct
     1321 ccggagtcgc cggccgcgcg catcctcggg ctgctggatg gcctgggtct gaactacaac
     1381 acggcgaagt cggtggatgg gctccgggcc gcgtttccgg gcaagttcct cttcgagtcg
     1441 gagtcctcgt cggagacgtc cacccggggc gtgtaccagg acccccacct gctgaacacc
     1501 ggtgagaact acaccccggg caagcggggc acgtcctcct acgacaacaa cctggcctcc
     1561 tggaccatga gtggggagta cggcctgaag aaggaccggg accggaagtt cttcgccggc
     1621 gagttcctct ggtcgggcat cgactacatc ggcgagccga ccccgtacga cgtctatccc
     1681 gtgaaggcct cgttctttgg cgccgtggac acggccggct tccccaagga catgtattac
     1741 ctgttcaaga gccagtggac ccaggagccc atggtccatc tgcttcccat gaactggacg
     1801 gaccacgcgc ctggccagcc cgtggctgtc tgggcctatg ccaatgtcga tacggtggag
     1861 ctgctcctga acggccagtc cctgggcgag cggaagttca gccacaagaa gaccctggat
     1921 ggccgggcgt atctggagac ggacgaggcc accggtgacg acaagacatt cacctcgggc
     1981 cccttcccgg gcagctacac cagtcccaat ggcagtgccg gcaagctgca cctgtcctgg
     2041 agcgtgccgt tcgcgccggg agagctggtg gcggtcgcca agaaggacgg cgtggaggtg
     2101 gcccgggacg tgctgcggac cgccggagcg ccgaacacct tgaggctgac cccggacaag
     2161 acggtcatca ccgcggacgg caagtcgctg gtgttcgtga cggccgaggt cgtggactcg
     2221 aagggggtga tggttcccag cgccgcgaac ctgattcact tccaggtgac gggcggagcc
     2281 ctggtgggcg ccgacaacgg gcgtcaggag agcgcggaga actacaaggc ctcttcccgg
     2341 acggccttca acggcaaggc gctggccatc atccagtcca gcgtcacctc cgggcccatc
     2401 accgtcaccg cgtcatcgga cgggctgact ccgggctcca cgacgattgt ctccgagggc
     2461 gggagtggtt ccgtggctgg ctccgcggcg acctcgactc cggtgggccc ggccaggagc
     2521 gtggggaccg tgccggcagc ggatccggaa atccccaccg ctgacgcgag ctactcgggc
     2581 tctccccagt cggtgcccgc ggcgatgctc gacgggaaca agacctcggg gggctggtcg
     2641 aacttctatg agaaggcggc caccgcgctc ctgcccgcgg tgagccaggc caacgcgtcg
     2701 gactgggtct cgctcaagtg gccccaggcg cggtccctcg attccgtgac ggcctggttc
     2761 acgaccggcg cgaagcgcgt gctgcccgcc tcgctcgtgg tgacctactg ggacggcaag
     2821 ggctacgtgc cggtctccca cccctccatc tcctgggcgt ccgcctcgaa ccagcccacg
     2881 gtgatctcct tcgacccggt ccagaccacc gagctgcggc tggagatgac gagccccgct
     2941 ccggggtccg ccaccggctt cctgcagatc tcggagctcg aggtgccagt gagccgctga
     3001 tgattcatgc ggtattggga tgagttcatg gcgtccgact cccccgctcc ctcactcacg
     3061 gtgtcgaagg tctcaacacc atgagtgggg gagttgcacc gtgcgttcat ctcggcgtcc
     3121 gcggagccgc tgctcgccgg gcgtcgcctt gcgatgctcc ttcacttctt tcgagaatgc
     3181 ggctggcccg ggttggcctt gggcattcac acacggagca ggctcatgaa gaggttggag
     3241 aacaaggtcg ccgtcatcac tggcggtggc ggcgggattg gcgcggcgac ggcgaagctc
     3301 ttcctgcagg agggcgcccg ggtgctgatc gtgggccgca ccgaggagaa gcttcgcaag
     3361 acgctccagg aactccacca cgagaacctc ggctacgccg tcgcggacgt gtccagggtc
     3421 gaggacacgc ggcgctacat ccagcaggcg gcggagcgct tcggcggcgt cgacgtgctg
     3481 gtgggcaacg cgggcgccga gggcccctac aagcccctgg acgagcactc cgtggaggac
     3541 ttcgacgagg tgatggccgt caacgtgcgc ggctgctggt tggccgtcaa atacgcgttc
     3601 cccgagatgc gcaagcgcgg aggcgggagc atcgtcctca cctcctcggt gatggggctc
     3661 gtcgcgtttc cgtccaactc ggcgtacacc gccgccaagc acgcggtggt gggcctggcg
     3721 cgctcgctgg cgcacgacgg cgcgccgttc aacatccggg tgaacgcggt gtgcccgggc
     3781 gtcatcgaca acgacatgac ggcgggtatc cacacgcggc tcgcgcccgg caacgaggac
     3841 gtggtgaaga gccggatggc tcagcgaatc cccatgcagc gctacgggac gaacgaggag
     3901 atcgcccgtc tgaacctctt cctcgccagt gacgagagca gctacagcac gggtggggtg
     3961 ttcgtgtcgg acggcggcat gacagccggc gtgcgaggcc ggtgagccac cccggagccg
     4021 gagcctgagc tccggccccg gagccgcggc aggtgttccg cagtgggtgt caaagaactc
     4081 gtgccaacac gtcgcgcgct acgagtcgtt cgacaccttc ttcagacacc ttcctcagtc
     4141 gcagtcgatg tcgttgaagg agttgtaggg ctcgccagcg acgctctcgg tctcgctgtt
     4201 gccgcaggcc gcggtgatcg tctgggcgtc cttggtcgag atccaccagg agtcgatgcc
     4261 cctgaactcg tcgtcgatgt tgccagcggc caggccggag atgttgcagc cgatgcccgt
     4321 ggtgcagcca ccgaggccgg gcatgcccgg gttcgcgccc tcgccctcgt acgtgggcga
     4381 ggacggcggg gtgaccttgg gcgtcaggac gttgttcggg aactccgccc tgtcgacggt
     4441 gatgcagttg gcgttcgggg agtcggtgac gcccgacgca ttgcggatga tgcagttcaa
     4501 ggcggacccg aagtagtagg cgtagcggtt gccactctcg ggcaggaagc ccacggtatg
     4561 cacgttctcc gagtatctgc cgttctcctc gtggtaggcg cgctgagcgg agaaccaggc
     4621 cttcaagttg cctttggcct ccccctgcgc ggcgagggcc tgccgcttga ggaagttcgg
     4681 gatggcgatg accgcgagga tgctgagcat ggccgtcacg atcgtgaatt cgatgatcgt
     4741 gaagcctcga tgcttgcgaa tgttgggacg gtgcatggtg ttccttctgg gatgtgcggt
     4801 gggtttgacc gcgtcccttc aaggggttgg actgcgtgtg ccggcttgag catgagtcat
     4861 gccagggcag gcggacggcc aagcccttgg aatgaggcgt aacccccccc tgttctggca
     4921 gcgggaagtg acgtttttcg gctgacggat gacacccaac gtcatcgccg actccggcag
     4981 gccaaccgtg gcttcctgaa ctagcgcctc tcctcgtgtg caccgccccg ggtgtcctgg
     5041 gccgggtcga gggcggacca gcgcgatgcc aggacggcga gcaactcgac catgcgcgga
     5101 tcgaaggtct cgaggtatgg cgcgagccgt gcgccggcga tgccctggct cagtcgcgac
     5161 aggatgaccc ggctctcgga ctcctcgacg agggccgtga ccagctcgcg ctgatagagg
     5221 gcaagctcct cgacgtcgag cgggtcgggg agtggcgtgg tgctggcgtc gcgcaccgga
     5281 ctgacgggcg ttgcactggc ctcggtcacc acttcgacca cggcacggta ctgggcgcgc
     5341 cactcgtctc gcacggccct cgactcgagg gtttcgcttc gtcgttcgac gatgacgacc
     5401 tcgcaatggg gacagaggtc gagcgcccgg cggagcaggg acagcaccgg cggcagcagg
     5461 ggtccgtcat gactgtcgag ccggacgggc ctggggtctc cggcgcggag cgcacgagcc
     5521 caactcccac ccgagacgtg gatctcccgg acgcactcca gcggatgggt ttcgagcagg
     5581 agttctggtg gcaggccggt gttgatcgcc tgggtccaca cgttgtggat gtcgagcacc
     5641 aggaagccgc cggtgggggt caggatggca tcgagaaacg caccctggtg gagggcatcg
     5701 gagggcgcga gtgtgttggc cagcacctcg aggcccacgg gaccgccggt ggcggcgcgg
     5761 agcctcccga gtcggtcccg gccgatgtcc acggcggcgg cgcaatacgg caggggcagc
     5821 atggtgctgc gggtgaacgg ccccgcggcg gtgaagccga agtgctcgga gacgtggcgg
     5881 tagggtctct ggcggcactc ctgggcgagg cgctcgagcc atgcgtcctg ccgtggctgc
     5941 cagtcggcgg tcagcaccga caaccacacg ccgtgcccgt acagggctcc ctcctccgca
     6001 tagaggtcga gcatgcgctc ggtccagtgg gggggcggag tcctggccga gaatccccag
     6061 gagtcatcga tgtcccactc gagcgcggtg accaggcctt cggcgaagag gggccgtgcc
     6121 gcctcgcgga aggcgtcgtc ggtcaagaga ttcaaaccca cgcgaggagg catgggggta
     6181 gctcgtcgtg ctcagcggcc cgcgtcggtg acgcgctggg tgctggactc tggcaacgcc
     6241 atcatcatgc cgcaggccgg acagggagtc gggcgggcat tcctttcctt ggtgttgttc
     6301 gccatgtcga ggagctgggt ggcttgctca cggaggttct gattcatggt tcttttcctt
     6361 tcctgcttgt gggggagccg gatgcgtggc acctgcccgg ctcctcggga aaggtggagt
     6421 cattgctggc cggatgtgct gaaaaagagg cgagcgttcc gtcaggcggc aggagagaag
     6481 cagggtctct tcattggctt ccggctttcc accaagcgct cggcgggact cccggcgcga
     6541 gggtgaggga gggcgaggac tcctccctcc tcgggcccag atgaaaatgg gtaaggtcga
     6601 gggagcgggg gatggcgcac tgggacgtgt gtcacgtttg cccgcgcgag tccggagggg
     6661 aacgctggta ctcctccacg taggttttca gcagctcggc ggctcgggag tgctcgcctc
     6721 cacactccat cagcaatccc tctccgtacc acgtcaaccc ctcccggaag agatctggac
     6781 ggcgccagcc aagccagcgc tcgaactggt gccactgttc accggcctcg gactggagcg
     6841 ctccggtcat gaatccctcg agcaggagtt tcaggttgtc caggtccggc tgcccatggt
     6901 cactcacggt ggccagggac tcggaggggg tcgagcacca gtggaccacc gactcgagga
     6961 tggtgtgggg cttcacttgc gcacctggcg tttcctggct gaccgtgcgc tctgccgcgg
     7021 gcaatgcctg gtactcctcc acgtaggccc gcaggcgctc agcggctctg gcgtgtgtct
     7081 cgccgcagtc aagcaagagc ccctctccaa accacgtcac tccttgcctg aagagccctg
     7141 gatggcactt gccgagccag agttcgaact gacgccattc ctcatgagac cgctgccgag
     7201 ggtccgcgca ctcgaatccc gccaggagca ggagcaggtt gtgaaggtct ggctgcccac
     7261 ggtcacgcgc cgtcaagagg aacatatatg ggcgggagca ccagcggagc actgcgtcga
     7321 gaatcgtgtg ggaagacatt cagcacctat tccaattctc ggaagaggct cggaaacgag
     7381 gcatattcct tggaggctat ctcttgtccc gtgtacgtgt ggatgcggcg aaggtattct
     7441 gcctcatcca tccctcccgg tccggtgtat gcgtcaaaaa cgcgcccctc gaacttgacg
     7501 gcaatgtgga cgccgttgtc ggcaagctgc ttcgcaaccc cagtgcgaag ctcgaaccca
     7561 aggatgagtc gtttctccgt tgagatgatc ttcaagaagt aggggcgact tcccatgaat
     7621 tcacccaggc ggtggagtac ttggaaacca ccacgcgcta cgtctccaca ttgcccggcg
     7681 atctggttcc cctcatcgaa gaagtgcgtc agtccgttga tgattgcctg ttgttgcgtg
     7741 acgctggcac ctgtcagggc ggagaggtag tacatcgctg ttgccatgtc ccctcgcgcg
     7801 agcgcggcga acatgttcgt ggcagcggcg tggtagctgg agcacaccag cgtcgccttc
     7861 ggctcctgag gacagttcac ctctccgttt cttcgtgggg atggcggtgt cgtcacgcaa
     7921 gcggtcaaca ggaacaagac agccagggtg caagcccatg atgtcctttg cctcgttttc
     7981 atgctgtgga acctagcgta aattaatcga caaactcaaa tggccttcgc atggcagcac
     8041 aatgccatga tattggaagt tgaagtgcgc taggttcctg tcaagcagag gcgggctgct
     8101 gctcggccgg cgtgctcccg gagagcaccg cccgggccga gtcgatggcc tcgtgcgtgc
     8161 cggccagcgc gagcacgtct ccctcccgca actgctcgtc cgcggtgggc acgacgacct
     8221 tgtcctcgcc gcgccggatg gcgagcaccg tggcgcccgt cattccgcgc aggttgagct
     8281 cggcgagcgt gcgccccacg gcggcactgc cggactcgag ccgcaccggc gtcggctcgc
     8341 cgagtccggg caggatggcg tgcagggtgt cgaggctctc ggcatgatgc gcctggggtt
     8401 tgccccggga ctgggacgcg agcgcctcga cgatgacctg ggagcccgcg cgcacgtgcc
     8461 cctggaggtt ggtggcgctg cgccagaagc ccatgccgag taccgcgagc aggatcagca
     8521 gcaccgccgc gccgaggaat ctgggcagga agggctgggt gatggcgacg agcggcgcgc
     8581 ccacgcccag cacgctcgcc aactggagcg tcaccacgag cgcgcgccgg ggggccgcgg
     8641 cccggtccag acggcccgtc acggagggga aggccgtctc cgccagcagc gtgcccagcg
     8701 agctcgcgag ccgtaccacg cccacgcaga agggggtggc gagcatggcg gccaggaaga
     8761 tgacgagcca gcgcgccacg gtggcgccca ggccgctgcg cagctccagc gccgcgctca
     8821 tgcgcccgag gaagagcgag gtgccgatga tgatggcggc gagcagggcc gcgtccagga
     8881 gcatcaggcg caccttggag cggatcttcg tgccggcggt ctggcgcggc ggcgcgttgc
     8941 gcagctcctc cacccaactg ccatagagcg ccgcgaaggt ctgcaagggc ttgggcagct
     9001 tgcggtccac gtagttggcc accggccccg aggcccggat gaggaagggc gtggtgaggg
     9061 tggtgatcgc cgacacggcc acggccaccg ggtagaggaa gctccccgtg gcgttgagcg
     9121 acaggcccag gcccgcgatg atgaaggaga actcgccaat ctgcgccagg ctcagtcccg
     9181 actggatggc cgtgcgcgtg ccgctgcccg tgaggaacac ccccagcgcc acccccacca
     9241 gcttgcccag gatgaccaca cccgtgagca ccgccaccgc cgcccagttc tcggcgatga
     9301 gccccggatc gatgagcatt cccaccgaca cgaagaagat ggcggcgaac atgtcgcgta
     9361 ccggctgcac caggtgctcg atctcctgct cctctccgga ctcggccacg agcgagcccg
     9421 cgaggaacgc cccgagcgcc accgagtagc cgaacgcctg cgcgagcagg gcgatggcga
     9481 agcagatgcc gatgctcgcc accagcgtcg tctccgggcg cttgagccgg tgcaccgcgc
     9541 gcaccgtgcg cggaatgacc atcagcccca ccaccacgag ccccaccagg aagagcgcca
     9601 ggcggcccac ggtgagggtg agctggcccg cggacagtcc cgcgcccgtg gagatggccg
     9661 tgagggtcgc catgagcagg atggcgatga gatcctccac gatgaggatg ccgacgacga
     9721 tctcccgcag cttgcctttg attccctgct cgtcgaacgc cttggcgatg atggtggtgc
     9781 tggagatggc gatgatggcg cccgtgaaga tgctctcccg ggacgtccac ccgaaggagc
     9841 ggcccaccac gaaccccagc cagatcatca ggctgcactg gatgatggcg gtgagtcccg
     9901 cggtgaatcc caccttgagc agcttgccca ggctgaactc cagcccgagc gagaacatga
     9961 gcaggatgac ccccaactcc gacagcgtct ggacgatggt ggggtccgcc acgaggggga
    10021 tgggcacgtg gggccccatg atgagccccg cgatgatgta gccgagcacc acgggttgcc
    10081 gcagccgctg gaagatgacg gacgtcaccg ccgcgacgca caacaccgtc gccagggcct
    10141 tgaggaagtc gtgtgcatcg tgcatcgcca ggtcaccgcc agagagggaa gccagacaac
    10201 gggaataact cgaagggggg accggtgctc gtccaaagga ccggccctcc ccccgtcttc
    10261 aacaactcag ccgagcgcct cgtaggcgat ggccgcgcca atgatgaaca agcaggtcag
    10321 gacggccacg atgagccggc ccgtctgcca gcgctcgtac tcgacgggcg ccggggaagg
    10381 gggttgcgga gtgggggtgg acgcagaggg gttctgttct tcgcgcaagg ggctgcctcg
    10441 ggcgcgccag caccggccgg cacgccggaa agggaaaatc tacctcgggg atcgcgacct
    10501 cacgcgagcc gggggggtcc ttgcgcggga cagttctcga tgagcgggcg atccgcgatg
    10561 cgtccatggc gtcgtgtcgg ctcgcacggg cggtgcatcc gcgtgagcgg cgtgacgtcc
    10621 gtctccaggc gcggcgcgag gccatgccgg gaatcgaatc gcttgtagcc ggggaggggt
    10681 ttgaccgcgg ggcggggcag ctccagggcg ccgtggagcg gtgcttcacg gtctcccacc
    10741 agccgctcgt gctgggggcg ccgggtcgcg gagaccgagg cgcttcgcgc cggtcgcgcg
    10801 gtgctggcgg cttcgtggac ctcgctccgc cgctggacgc tggacgaggg ctccccggcg
    10861 aggacggcca ggaggagggc gcaggccgcg ccccccagga ccatccaccg ggcgagtcct
    10921 tgttcgcgcc gaggtgagcg gggagaggtc ttcatgagcg gagagctgac ccgttcatcc
    10981 ctacaccatc cggaacgcgg cgggcaaagg tgctcttcac gcggggccgg ttctctccct
    11041 cccggccccg ctcaccgcgt cgtgcccggc ctagttgcgc gtgccggagc ccttggggcc
    11101 gatggcgccc gggccggtgg tgcccgggcc gttggtgccg ctcggaatgc tgttcggagt
    11161 gccaatggtg cca
//
