LOCUS       NZ_CP022098            10831 bp    DNA     circular CON 17-FEB-2024
DEFINITION  Cystobacter fuscus strain DSM 52655 chromosome, complete genome.
ACCESSION   NZ_CP022098
VERSION     NZ_CP022098.1
KEYWORDS    RefSeq.
SOURCE      Cystobacter fuscus
  ORGANISM  Cystobacter fuscus
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Archangiaceae; Cystobacter.
REFERENCE   1  (bases 1 to 12349744)
  AUTHORS   Rupp,O., Goesmann,A. and Sogaard-Andersen,L.
  TITLE     Sequencing and comparative analysis of myxobacterial genomes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12349744)
  AUTHORS   Bruckskotten,M., Treuner-Lange,A., Rupp,O., Goesmann,A. and
            Sogaard-Andersen,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUN-2017) Department of Ecophysiology, Max Planck
            Institute for Terrestrial Microbiology, Karl von Frisch Str. 10,
            Marburg, Hessen 35043, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 09-AUG-2016
            Assembly Method                   :: Celera Assembler v. 8.1
            Expected Final Version            :: yes
            Genome Coverage                   :: 62.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/17/2024 10:04:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,093
            CDSs (total)                      :: 10,016
            Genes (coding)                    :: 9,906
            CDSs (with protein)               :: 9,906
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 26 of 110
            Pseudo Genes (incomplete)         :: 101 of 110
            Pseudo Genes (internal stop)      :: 1 of 110
            Pseudo Genes (multiple problems)  :: 17 of 110
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:24:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 84765
            Orig. end                         :: 95596
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10831
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10831
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10831
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5831](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5831
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            115..1209
                     /locus_tag="CYFUS_RS00355"
                     /old_locus_tag="CYFUS_000071"
     CDS             115..1209
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631007.1"
                     /locus_tag="CYFUS_RS00355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_000071"
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_095983383.1"
                     /sec_met_domain="ADH_N (E-value: 6.3e-21, bitscore: 73.5,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 4.4e-09, bitscore:
                     35.6, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAVVLFPKEGKVKVIDVPEPRLGSPGQVRVRTLEVGVCGTDREV
                     ARGEHGRAPEGEDYLIVGHECLGEVVEVGAEVKDLKPGDLVVPRVRRPCASEQCTPCRE
                     GHADFCVTGEYTERGIQGAHGFCAEFFVEDARYLHRVPRELREVAVLTEPLTIAEKSLR
                     QVKLIQQRVPSKDERASHAVVLGAGPVGLLGAMALVRAGYQTTVYSHSRKPNKKAELSE
                     AVGVPYVSSEEYSVKELVKQRGRADVVYEAAGVASAAFALLEGLAPNGVFVFTGVPPSE
                     KQKVDEGALMKRVVLDNQVLLGTVNAAAEDFDAAIEDLFHFQSRWRGPLESLITARHPP
                     EDYAEVVEGDKKSGVKDVIAFSRG"
     gene            1301..1939
                     /locus_tag="CYFUS_RS00360"
                     /old_locus_tag="CYFUS_000072"
     CDS             1301..1939
                     /GO_function="GO:0004061 - arylformamidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /locus_tag="CYFUS_RS00360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_000072"
                     /product="cyclase family protein"
                     /protein_id="WP_095983384.1"
                     /transl_table=11
                     /translation="MQGAWLDISIPFAEEPSGQEVVAGRTGMPESAAEQAEWLKRAAWM
                     GTYVTAPPSLELDLEDTERLPLSATVGKARVLHLDDVDCIRADSLAEYEPREGERLLLR
                     TRNSSREWWKRPHGEDFVMLSDAAAQLLVERRVACVGVDYVSRAGFHTEGLGVHQRLRE
                     AGMWLIEGLDLSEVKVGVHELVCLPLKVKAQWGSPARALVRTLREASLG"
     gene            2069..3928
                     /locus_tag="CYFUS_RS00365"
                     /old_locus_tag="CYFUS_000073"
     CDS             2069..3928
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631009.1"
                     /locus_tag="CYFUS_RS00365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_000073"
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="WP_095983385.1"
                     /transl_table=11
                     /translation="MNGEKTARSPAIEDHGVIGDLRTVALVATDGTLDWLCFPHFDSPS
                     VFAALLDADKGGHFFIRPERLPGQPEVVHKQFYWPDTNVLVTRFYSENGVGELVDFMPL
                     GTQAPVRMVVRRIRVVRGEMPFRMECLPAFNYARDEHSVRMLDHGVSFLSPTLSLTLAT
                     KAKLETDGKRVTSRFTLREGQSMVFSLIEGAPSSCARQAMGHESAEELFRDTVSYWRHW
                     LSQCTYRGRWREVVYRSALALKLMTFEPSGAIVAAPTCSLPESPGGGRNWDYRFVWIRD
                     AAFTVYALLRIGFSDEAGAFMRWLEQRIAELGENEPLPLMFALDGTKVPLEEELSHLAG
                     YRDSRPVRIGNEAAHNHLQLDIYGELMDGVYLYNKHGAPISYDFWSHLRRLMNWLCDNW
                     RRKDESIWEVRSGRQHFVYSKMMCWVALDRAIRLAGKRSFPSDLARWIHERDAMFEEIM
                     NEGWCQERSTFVQYYGAQALDASVLLMPLVFFLSPVDPRILSTVELIRKPPKEGGLTSD
                     GLVFRYDTEASQDGLTGREGTFNLCTFWLVEAMTRASVTRPDLLDEARLLFERMLGYAS
                     HLGLFAEQTGPAGEALGNFPQALTHLSLISAAYNLDRALGAGD"
     misc_feature    3973..3988
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 19.52"
                     /tool="antismash"
     gene            4069..5004
                     /locus_tag="CYFUS_RS00370"
                     /old_locus_tag="CYFUS_000074"
     CDS             4069..5004
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631010.1"
                     /locus_tag="CYFUS_RS00370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_000074"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_095983386.1"
                     /transl_table=11
                     /translation="MNPLDWNGQQFLAMYGPFLLFVCVGAVIWKRLLNRPSDVPTPAEL
                     DLNPYLAATLESEDTAVRAAVVALVHDGALKFGEEDEELSVAKALPTRALELERVVHAA
                     VAQGKTSLGELREAAQTELSRIVQSLRARGFMRTPEQDSRYRHHPRLFLLAALALGVMK
                     VMVGLSRDRPVGFLVLQILLGGGVGFALLSGGSRLTPRGREALRLLRKRHEPLRTTAAS
                     EGSAQTMSTRDVALAMALFGVGGLALVGGHSLLRDYLFPPSPAGGGGSGGDGSSSSSSS
                     SCSSSSSCSSSSSCSGGSSGCGGCGGGGGD"
     gene            5001..5831
                     /locus_tag="CYFUS_RS00375"
                     /old_locus_tag="CYFUS_000075"
     CDS             5001..5831
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631011.1"
                     /locus_tag="CYFUS_RS00375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_000075"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_095983387.1"
                     /sec_met_domain="DUF692 (E-value: 1.8e-76, bitscore: 256.1,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRLEGVGVGWRRQLAHFLDTWEGLGFVEVLAEQFDDRGALPEPL
                     LQLKERGVPVVVHGVSLGLGGAEPPSPARLSRLARLAERLGAVCVSEHLAFVRAGGLES
                     GHLLPVARHEQSLEVLADNIRRAEAALPVPLALENVASLFEWPAPAFSEAGLLTGVLER
                     TGAGVLLDVANLYANALNLGTDARAVLAAVPRERLFYVHVAGGVRHGGLYHDTHAHALP
                     EGPLALLEELTARVGAVPALLERDDRFPPVEELVGELEAIRGAMARGVARRETA"
     gene            5828..6316
                     /locus_tag="CYFUS_RS00380"
                     /old_locus_tag="CYFUS_000076"
     CDS             5828..6316
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350082.1"
                     /locus_tag="CYFUS_RS00380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_000076"
                     /product="hypothetical protein"
                     /protein_id="WP_095983388.1"
                     /transl_table=11
                     /translation="MSRAREELARAQAELMRALELGEPVPTGFDAERVRATADTLLAKR
                     RRGVRRAWPVLERALGEAFAGEFDAWARAHPPRAFEPHAGVEGYRFALALHDQGRLPRE
                     AEAEVLGFGVRWRITPTGGLAPRWGLVFRMLRMGERRRWNWAVRLPGGRVLRGRLPGG"
     gene            complement(6302..6913)
                     /locus_tag="CYFUS_RS00385"
                     /old_locus_tag="CYFUS_000077"
     CDS             complement(6302..6913)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /locus_tag="CYFUS_RS00385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_000077"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_095983389.1"
                     /transl_table=11
                     /translation="MGRPRQVLDEEILEAARACFIEHGASVSTETIASRLGVSGPALLK
                     RFGSKRELLKAAFAVGSAPPWLPMVEEGPDERDISVQLRELALTIDAFFRKMVPAFSVL
                     REAGLTPQEIRGDSEDSPAARAQDSMTGWFKRAQERGLLREGDPSLMAGLFMAGVQYRY
                     FLAHITQQRVPTEEEDPFVERLLNMLWRGIAPPDQASPAR"
     misc_feature    7114..7128
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.54"
                     /tool="antismash"
     gene            7456..8097
                     /locus_tag="CYFUS_RS00390"
                     /old_locus_tag="CYFUS_000078"
     CDS             7456..8097
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631014.1"
                     /locus_tag="CYFUS_RS00390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_000078"
                     /product="YdcF family protein"
                     /protein_id="WP_095983390.1"
                     /transl_table=11
                     /translation="MPPLALRQTPGSLRRGLLLGLGAMTGGLFGLAFVVDRFGQKERAV
                     AAEVVVVLGARVLPGGEPSSALRARVERAVALYHQGLAPRLLFSGGVGLHPPAEAHVMR
                     ELAMRLGVPGHACLLEDQSRSTEENARLCARVLGTLGVRRAVVVSDPFHLLRARQYFRL
                     HGLEVATSPALVPGRALRATDRFYWTLREAAALLLHPRVLLARAPTRARG"
     misc_feature    8110..8122
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.62"
                     /tool="antismash"
     gene            8152..9315
                     /locus_tag="CYFUS_RS00395"
                     /old_locus_tag="CYFUS_000079"
     CDS             8152..9315
                     /GO_function="GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631015.1"
                     /locus_tag="CYFUS_RS00395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="CYFUS_000079"
                     /product="phosphatase domain-containing protein"
                     /protein_id="WP_095983391.1"
                     /transl_table=11
                     /translation="MTRKRPNALVRLAFQVEHHAETLSWRLRHKLRLARPPRILPYRGY
                     GTTRASLIKARVLEDRKVRPPWKRYTLVGSAIASWKRYMTLEIPNARVAVRWGDKRWEG
                     TTDEEGFLELWVEPPEGVKAGWHDVELELLSPVSRRGVTRVCAPVLVSGPEAELGVISD
                     IDDTVIVTEVTNVLKRFWTLFLTEHRTRLPFEGVDAFYQALSQGSTGSAYNPIFYVSSS
                     PWNLYEHLDEFLGLHHIPAGPLLLRDWGLSRTGFAPGGGHGHKLEKIRGLMDAFAPLPF
                     LLIGDSGQEDAEHYRTIVREYPGRVRCVYIRNVWHRSGRERELAAIAEDIRAAGSQMLT
                     VDDTVTAARHAASQGWIRRQEVAEVQAHRQEDLQARTALDALDREPS"
     misc_feature    8175..8193
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.57"
                     /tool="antismash"
     misc_feature    complement(9331..9345)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.95"
                     /tool="antismash"
     misc_feature    9332..9345
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.51"
                     /tool="antismash"
     misc_feature    complement(9332..9347)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 21.02"
                     /tool="antismash"
     misc_feature    9383..9397
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.91"
                     /tool="antismash"
     gene            9415..9957
                     /locus_tag="CYFUS_RS00400"
                     /old_locus_tag="CYFUS_000080"
     CDS             9415..9957
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CYFUS_RS00400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="CYFUS_000080"
                     /product="hypothetical protein"
                     /protein_id="WP_095983392.1"
                     /transl_table=11
                     /translation="MMRHVMKTLSLVSVAVLAACGGGVETSREEPAAQVGQGLAELSLS
                     EGSITSALQSTLNTGNAKYRWTEGSLRRFDFSGSATLAQADAAVLGFQEYEAYAYFGST
                     QAISYADWKNTLYSEFQPLHSQILSTYSNGTETVQVVTHYYEQLVAAGSTGWFRLTVIL
                     FPQSRKVIVFEQTGYEV"
     misc_feature    complement(9427..9442)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.57"
                     /tool="antismash"
ORIGIN
        1 tgaccggtgc gcatccgggg agaccgagtg tccaccaccc ggaggggatg ggcccaaggg
       61 ggctgcgcca gggggcgagg tgatgcacga caggggccca cgggaggtgg tgcgatgaag
      121 gcggtggtgc tgttccccaa ggaagggaag gtcaaggtca tcgatgtgcc cgagccgcgg
      181 ctgggctcgc ccggccaggt gcgggtgcgc acgctggagg tgggggtgtg cggcaccgac
      241 cgggaggtgg cgcggggcga gcatggccgg gcccccgagg gagaggacta cctcatcgtc
      301 ggccacgagt gcctgggcga ggtggtggag gtgggcgcgg aggtgaagga cctgaagccc
      361 ggggacctgg tggtgccgcg ggtgcgccgg ccctgtgcga gcgagcagtg cacgccgtgc
      421 cgcgagggcc acgcggactt ctgcgtgacg ggggagtaca cggagcgggg cattcagggc
      481 gcgcacggct tctgcgcgga gttcttcgtg gaggacgcgc gctacctgca ccgggtgccg
      541 cgcgagctgc gcgaggtggc cgtgctcacc gagccgctga ccatcgcgga gaagagcctg
      601 cgccaggtga agctcatcca gcagcgggtg ccctccaagg acgagcgggc ctcgcacgcg
      661 gtggtgctgg gcgcggggcc ggtggggttg ctcggggcca tggcgctggt gcgcgcgggt
      721 taccagacga ccgtgtactc gcattcccgc aagcccaaca agaaggcgga gctgagcgag
      781 gcggtgggcg tgccctacgt gtcctccgag gagtactccg tgaaggagct cgtgaaacag
      841 cgggggcggg cggacgtggt gtatgaggcg gcgggcgtgg cgagcgccgc cttcgcgctg
      901 ctcgaggggc tcgcgcccaa cggggtgttc gtcttcaccg gcgtgccgcc gagcgagaag
      961 cagaaggtgg acgagggcgc gctgatgaag cgcgtggttc tcgacaacca ggtgctgctc
     1021 ggcacggtga acgccgcggc ggaggatttc gacgcggcga tcgaggacct gtttcacttc
     1081 cagagcaggt ggcgcgggcc gctcgaatcc ctcatcaccg cgcgccatcc tcccgaggac
     1141 tacgccgagg tggtggaggg cgacaaaaag agcggcgtca aggacgtcat cgccttttcc
     1201 cgagggtgag aagcgggtga caatgcgtcc atcgcctcgc ggggggaggg gaattagggt
     1261 gtggtgctgg gggcccgtct gaccactcaa ggaggaacgc atgcaaggag cctggctgga
     1321 catctcgata ccgttcgcgg aggagccgtc cgggcaagaa gtggtcgcgg ggcggacggg
     1381 gatgcccgag agcgccgcgg aacaggccga gtggttgaag cgggccgcgt ggatggggac
     1441 gtatgtgacg gcgccgccga gtctggaatt ggacctggag gacaccgagc ggctgccctt
     1501 gagcgcgacg gtgggcaagg cgcgcgtgct gcacctggat gacgtggact gcatccgggc
     1561 cgactcgctg gccgagtacg agccgcgcga gggagagcgg ttgctcttgc gcacgcgcaa
     1621 ctcctcgcgc gagtggtgga agcggccgca cggcgaggac ttcgtgatgc tgtcggacgc
     1681 ggcggcgcag ttgctcgtgg agcggcgcgt ggcgtgcgtg ggcgtggact acgtgtcgcg
     1741 cgcgggcttc cacaccgagg gcctcggcgt gcaccagcgc ctgcgcgagg ccgggatgtg
     1801 gctcatcgag gggctggacc tgagcgaggt gaaggtgggc gtgcacgagc tggtgtgtct
     1861 gcccttgaag gtgaaggccc agtggggctc tcccgcgcgg gcactcgtgc gcacgctccg
     1921 ggaagcgtcc ctcgggtagc cagccgcccg gcgcgctgga gggtcggcgt gcggacatct
     1981 ggaggattcc cagcgcgtcc gtgagcactt cgggctggcg gacgccgggg tccactccct
     2041 acttcgaagt acagcgaggg agaggcccat gaacggggag aagacagcgc gatcacccgc
     2101 catcgaggac catggtgtga ttggggatct gcgcacggtg gcgctggtgg cgaccgacgg
     2161 caccttggat tggttgtgct ttccgcactt cgacagcccg agcgtcttcg ccgcgttgtt
     2221 ggacgcggac aagggcggcc acttcttcat ccggccggag cgcctgccgg gccagccgga
     2281 ggtggtgcac aagcagttct actggccgga caccaacgtg ctggtgactc gcttctacag
     2341 cgagaacggc gtgggtgagc tggtggactt catgcccctg ggcacccagg cgccggtgcg
     2401 catggtggtg cggcgcatcc gcgtggtgcg cggggagatg ccctttcgca tggagtgtct
     2461 gcccgccttc aactacgcgc gggacgagca ctcggtgcgg atgctcgacc atggggtgag
     2521 cttcctgtcc cccacgctca gcctgacgct cgccacgaag gcgaagctgg agacggacgg
     2581 caagcgtgtc acctcgcgct tcaccctgcg cgagggccag tccatggtgt tctccctgat
     2641 cgagggcgcg ccctcgtcct gcgccaggca ggccatgggg cacgagtccg ccgaggagtt
     2701 gttccgcgac acggtgagct actggcggca ctggctgtcg cagtgcacct accgggggcg
     2761 ctggcgcgag gtggtgtatc gctcggcgct cgcgctcaag ctgatgacgt tcgagccctc
     2821 gggggccatc gtggccgcgc ccacgtgcag tctgccggag tcccccggcg gcggacgcaa
     2881 ctgggactac cgcttcgtgt ggatccgcga cgcggccttc acggtgtacg cgctgttgcg
     2941 catcgggttc tcggacgagg cgggcgcgtt catgcgctgg ttggagcagc gcatcgcgga
     3001 gctgggcgag aacgagccgc tcccgctcat gttcgcgctg gatggcacca aggtgcccct
     3061 ggaggaggag ctgtcgcacc tggcgggcta tcgcgactca cgtccggtgc gcatcggcaa
     3121 cgaggcggcg cacaaccacc tccagctcga catctacggc gagctgatgg acggcgtgta
     3181 cctgtacaac aagcacggcg cgcccatcag ctatgacttc tggagccacc tgcgccggct
     3241 gatgaactgg ctgtgcgaca actggcggcg caaggacgag agcatctggg aggtgcgctc
     3301 ggggcggcag cacttcgtgt actcgaagat gatgtgctgg gtggcgttgg accgggccat
     3361 ccgcctggcc ggcaagcgca gcttcccctc ggacctggcc cggtggatcc acgagcgtga
     3421 cgccatgttc gaggagatca tgaacgaggg ctggtgccag gagcggagca ccttcgtgca
     3481 gtactacggc gcccaggccc tggacgcctc cgtgctgctc atgccgctgg tcttcttcct
     3541 gtcgccggtg gatccgcgca tcctctccac cgtggagctc atccgcaagc cgccgaagga
     3601 gggagggctc acgtcggacg ggctcgtgtt ccgctacgac acggaggcca gccaggacgg
     3661 gctcaccggc cgcgagggca cgttcaacct gtgcaccttc tggctcgtgg aggcgatgac
     3721 gcgcgcgagc gtcacccggc cggacttgct ggacgaggcc cggctcctct tcgagcggat
     3781 gctcggctac gccagccacc tgggcctgtt cgccgagcag acgggaccgg ctggcgaggc
     3841 cctcggcaac ttcccccagg ccctcaccca cctgtcgctc atcagcgccg cgtacaacct
     3901 ggaccgggcg ctcggcgcgg gcgactgagc gctctcgggg ggccgacggc gtgccaggag
     3961 tgtcccgggg atgtcacggc gacgtcacgc gcgtccggtg tagagtgggg acacgcgctg
     4021 cccccattca ggcggagtgc cagggcgggg gggcgccggg agcgctcgat gaatccactg
     4081 gattggaatg ggcagcagtt cctcgccatg tacgggccgt tcctcctgtt cgtctgcgtg
     4141 ggggcggtga tctggaaacg cctgctcaac cggccgtctg acgtccccac gcccgccgag
     4201 ctggacctga acccctatct ggcggccacg ctggagagcg aggacacggc ggtgagggcg
     4261 gccgtggtgg cgctcgtgca cgacggcgcg ctgaagttcg gggaagagga tgaggagttg
     4321 agcgtcgcca aggccctgcc cacgcgggcg ctggagctcg agcgggtcgt gcatgccgcg
     4381 gtggcccagg gcaagacgtc gctcggggag ttgcgcgagg cggcccagac ggagctctcc
     4441 cggatcgtgc agtccctgcg ggcgcgaggc ttcatgcgca cgcccgagca ggactcgcgc
     4501 tacaggcacc atccccggct gttcctgctc gcggcgctcg cgctcggggt catgaaggtg
     4561 atggtgggcc tgtcccggga caggcccgtg ggcttcctcg tgctgcagat cctcctggga
     4621 ggaggggtgg ggttcgcgtt gctgagtggc ggctcccggc tcactccccg aggccgggag
     4681 gcgctgaggc tgctgcgcaa gcgtcatgag cccctgcgca ccaccgccgc ctcggagggc
     4741 agtgcccaga cgatgagcac gcgcgacgtg gccctggcga tggcgctctt cggggtgggc
     4801 gggctcgcgc tggtcggcgg ccattccctc ctgcgcgact acctgtttcc cccgagcccc
     4861 gcgggaggtg gcggttccgg aggggacggc tcgagcagca gcagctcgag cagttgcagc
     4921 agctcgagca gttgcagcag ctcgagcagt tgcagcggcg gaagcagcgg ctgcggcggc
     4981 tgtggaggag gcggcgggga ttgaagcggc tcgagggcgt gggagtaggc tggcggcggc
     5041 agctcgcgca cttcctcgac acctgggagg gactgggttt cgtggaggtg ctggccgagc
     5101 agttcgatga ccggggcgcc ctgcccgagc cgctcctcca gctcaaggag cgtggggtgc
     5161 cggtggtggt gcacggggtg tcactggggc tgggtggcgc cgagcctccc tcgcccgcgc
     5221 ggctctcccg gctggcgcgg ctcgccgagc ggctgggcgc ggtgtgtgtg agcgagcacc
     5281 tggccttcgt gcgcgcgggg ggcctggagt ccgggcacct gctgccggtg gcgcgccacg
     5341 agcagtccct ggaggtgctc gcggacaaca tccggcgggc cgaggcggcg ctcccggtac
     5401 ccctggcgct ggagaacgtg gcgagcctct tcgagtggcc cgctcccgcc ttctccgagg
     5461 cggggctgct cacgggggtg ctcgagcgca cgggggcggg ggtgctgctg gacgtggcca
     5521 acctgtacgc caacgcgctc aacctgggca cggacgcgcg agcggtgctg gcggcggtgc
     5581 cgcgcgagcg cctcttctac gtgcacgtgg cgggcggggt gcggcacggc gggctgtacc
     5641 acgacaccca tgcgcacgcg ctgcccgagg gacccctggc cctcctggag gagctgacgg
     5701 cgcgggtggg agcggtgccg gcgctgctgg agcgggatga tcgcttcccg cccgtggagg
     5761 agctcgtggg cgagctggag gccattcggg gggcgatggc ccggggcgtg gcgcgccggg
     5821 agacggcatg agccgcgcgc gcgaggagct ggcccgggcg caggcggagc tgatgcgcgc
     5881 gttggagctg ggggagccgg tgcccacggg cttcgacgcc gagcgggtcc gggccacggc
     5941 ggacacgctg ctcgccaagc gtcggcgggg agtgcggcgc gcgtggcccg tgctcgagcg
     6001 ggcgctcggg gaggccttcg ccggggagtt cgatgcctgg gcccgggccc atcccccgcg
     6061 cgccttcgag ccccacgcgg gcgtggaggg ctaccgcttc gccctggcgc tgcacgacca
     6121 gggacgtctt ccccgggagg cggaggcgga ggtgctcggc ttcggggtgc gctggcggat
     6181 cacccccacg gggggactgg ccccgcgatg ggggctcgtc ttccggatgc tgcggatggg
     6241 ggagcggcgg cggtggaatt gggcggtgcg gctgcccggg gggcgcgtgc ttcgggggcg
     6301 gctacctggc gggtgaggcc tggtccggcg gcgcgatgcc ccgccagagc atgttgagca
     6361 ggcgctccac gaacggatcc tcctcctccg tggggacgcg ctgctgcgtg atgtgggcga
     6421 ggaagtagcg gtattgcacc cccgccatga agaggccggc catgaggctc gggtctccct
     6481 cgcgcagcag gccccgctcc tgggcccgct tgaaccagcc cgtcatcgag tcctgggccc
     6541 gcgccgccgg agagtcctcc gagtctcccc ggatttcctg gggcgtgagg cccgcctcgc
     6601 gcagcaccga gaaggccggc accatcttgc ggaagaaggc atcgatggtg agggccagct
     6661 cgcgcagctg cacggagatg tcccgttcat ccggcccctc ctccaccatg ggcagccacg
     6721 ggggagcgct tcccacggcg aaggccgcct tgagcagctc ccgcttgctg ccgaagcgct
     6781 tgagcagtgc cggaccggag acgcccaggc gcgacgcgat cgtctccgtg ctcacggagg
     6841 cgccgtgctc gatgaagcag gcgcgtgccg cttccagaat ctcctcgtcc aggacctgtc
     6901 ggggacgccc catgaacccg cccgctgaat tggttagcta ttgctaacta actaaccccg
     6961 cgcagggggc ggaatcaagg gcacgtcaca tcggccaacg tcttgggagg ggtaactggc
     7021 gagcgacgag gcgagcgtga ggcgactggt cccggcggga cggtgcccca cttcccgtct
     7081 tcctggatgc aaggcctgtc ccgttttgga gcgtggacgt catttttacc gccccggacg
     7141 gggcccgggc gctcgggaga aacgtaagta cctggaatca cgagagcggc tgggggcacc
     7201 acccctggca cgcggagtgc tttacccctg ctcgagacgg gggatggtcg agggcggcgg
     7261 gtcggtgggc ggcgggttgg acgggcggtc aggtcgaggg tggcgggtcg gggattcgtt
     7321 tcaacgcttc aacatgggga gggcgtgcgg agcagtgcac tcgggggagt gcagggggaa
     7381 gtgagtcggg ggggagtcgc gggggcggct cctccccgat tccttttgcg ggcctttgct
     7441 agggtccgcc ggacgatgcc tccactcgcg ctgcgtcaga cgccaggttc cctccggcgc
     7501 gggctgctgc tgggcctggg cgccatgacc ggggggctgt tcggcctggc cttcgtggtg
     7561 gaccggttcg gccagaagga gcgggcggtc gccgccgagg tggtggtggt gctcggggcc
     7621 cgggtgctgc ccggaggcga gccctcgtcg gccctgcgcg cgcgggtcga gcgggcggtg
     7681 gcgctctacc accaggggct ggcaccccgg ctgctcttct ccgggggcgt gggcctgcac
     7741 ccgcccgccg aggcccacgt catgcgcgag ctggcgatgc ggctcggggt ccccggtcat
     7801 gcctgcctgc tggaggacca gagccgctcc accgaggaga acgcgcggct gtgcgcgcgg
     7861 gtgctgggga cgctcggggt gcggcgcgcg gtggtggtgt cggatccctt ccacctgctc
     7921 agggcccgcc agtacttccg gctccacggc ctggaggtgg ccacgagtcc cgcgctcgtg
     7981 cccggacgcg ccctgcgcgc cacggatcgc ttctactgga cactgcgcga ggcggccgcg
     8041 ctgctgctcc atccgcgggt gctcctggcg cgtgcgccca cccgggcacg cgggtgagaa
     8101 cccagggctg ggccattgtc accacgggcg gaagtcagta gctctaggtc catgacccgc
     8161 aaacggccca acgccctggt ccggctcgcc ttccaggtcg agcaccacgc cgagaccttg
     8221 agctggaggc tgcggcacaa gctccggctg gcgcgtcctc cacgaatcct cccctaccgg
     8281 ggctatggga cgacgcgcgc gagcctcatc aaggcgcgcg tgctggagga ccgcaaggtg
     8341 cgcccgccct ggaagcgcta cacgctcgtg ggcagcgcca tcgcctcgtg gaagcgctac
     8401 atgacgctgg agattcccaa cgcccgggtg gcggtgcgct ggggagacaa gcgttgggag
     8461 gggacgacgg acgaggaggg cttcctcgag ctgtgggtgg agcctcccga gggggtgaag
     8521 gcgggctggc acgacgtgga gctggagctg ctctcgcccg tgtcgcggcg gggggtgacg
     8581 cgcgtgtgcg ccccggtgct ggtgtccggc cccgaggcgg agctgggcgt catcagcgac
     8641 atcgacgaca ccgtcatcgt caccgaggtc accaacgtcc tcaagcgctt ctggacgctc
     8701 ttcctcaccg agcaccgcac gcgcctgccc ttcgagggcg tggatgcctt ctaccaggcg
     8761 ctgagccagg gcagcacggg ctcggcgtac aaccccatct tctacgtctc ctcgagcccc
     8821 tggaacctgt acgagcacct ggacgagttc ctcggcctgc accacatccc cgcggggccg
     8881 ctgctgttgc gggactgggg cttgagccgc acgggcttcg cgcccggggg agggcatggc
     8941 cacaagctgg agaagatccg cggcctgatg gacgcgttcg ccccgctgcc cttcctgctc
     9001 atcggggaca gcggacagga ggacgccgag cactaccgca ccatcgtgcg cgagtacccc
     9061 ggccgcgtgc gctgcgtgta catccgcaat gtgtggcatc gctcgggccg cgagcgcgag
     9121 ctggcggcca tcgccgagga catccgcgcc gcgggcagtc agatgctcac cgtggacgac
     9181 accgtcaccg ccgcgcgcca tgccgccagc cagggatgga tccgccgtca ggaggtggcc
     9241 gaggtccagg cccaccgcca ggaggatctc caggcgcgca ccgccctgga cgcgctggat
     9301 cgcgagccct cgtgaggccc attccttctc atgaaatgat tctcattcct gtcacttgaa
     9361 ttcaggggag gcgcgtgaca catggcattg gctttcaact cgagagggtg tccgatgatg
     9421 cgtcacgtga tgaagacgtt gagcctggtg tcggtggccg tgctggcggc gtgcggtggc
     9481 ggtgtggaga cctcgcgcga ggagccggcg gcccaggtgg gccagggcct cgcggagctg
     9541 tcgctgtccg agggctccat cacctcggcg ctccagtcga cgctcaacac gggcaacgcg
     9601 aagtaccgct ggacggaggg ctcgctgcgc cgcttcgact tctcgggctc ggcgaccctc
     9661 gcccaggcgg acgcggccgt cctcgggttc caggagtacg aggcgtatgc ctacttcggc
     9721 tcgacccagg ccatctccta cgcggactgg aagaacaccc tgtactcgga gttccagccg
     9781 ctgcactcgc agatcctctc cacctactcg aacgggacgg agacggtgca ggtggtgacg
     9841 cactactacg agcagctcgt ggcggcgggc tcgacgggct ggttccggct gaccgtcatc
     9901 ctcttccccc agtcgcgcaa ggtcatcgtc ttcgagcaga ccggctacga ggtctgagcc
     9961 agacgtctga gccagacaat gacgtgagga cgtgcgcggg cgccggtgtt cccctggggg
    10021 gagcccggcg cttcgtgttt cgtcacgggc cggcggcgtc ctcggagatg tgcagcgtgt
    10081 agcggatggg ccaggcggtg tcgctccggg ccatgccgtc caccaccacg agcaccgtgc
    10141 gccccttctc cagggtgagc ttcacccggg gcgcgcccgt gcggctcgac gcgcacgcga
    10201 gctcctggcc ccggcacccg gtgagcacgt acagcgcgtt gccgtcactg cccgaggtat
    10261 cgaagacgaa ggtgccatcc cggggcgcgg tccacaggtg cgcccgatcc tgctgaagga
    10321 gctccccgca cgtgccctgg aagccgtccc ccgcgtgcac cgtgtcgccg aggaacgtga
    10381 cgggcagggc gctgcccagg ttgatgtccg agcacccgcg cccgtcacac agcacgtctc
    10441 cctggcagtc cgtgtccgcg cagtccaccc accggtcccc gtcattgtcc gcgccgtcga
    10501 agcaactggc ggcctcggcg gccgtctgct gggtgatgtg cagctcgaag tccccctcgc
    10561 tgaagtcgcc accctggggg gagtccaccc ccaccagcac ccgctgtccc gcggtgaggc
    10621 gcaccgacac ccgggccccg ccgccatagc tgatgccacc cgtggcgcac gcgagctccg
    10681 cgcccccaca gcctccctgc cgcacgctca ccagggtgtg catcgccgag cgcgccgtgt
    10741 cgaagccgta ggtgcccgtg cgcggcgccg tccacaggaa gccccggtcg ggcgctccca
    10801 ggccgccaca ggaggcctgg tggtcatcgc g
//
