LOCUS       NZ_NDXL01000003        10405 bp    DNA     linear   CON 27-APR-2024
DEFINITION  Streptomyces kasugaensis strain BCRC 12349 scaffold3, whole genome
            shotgun sequence.
ACCESSION   NZ_NDXL01000003
VERSION     NZ_NDXL01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces kasugaensis
  ORGANISM  Streptomyces kasugaensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2433044)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Comparative genome analysis for kasugamycin overproduction
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2433044)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Comparative genome analysis for kasugamycin overproduction
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 2433044)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030,
            China
REFERENCE   4  (bases 1 to 2433044)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. May-2015
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 188.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002261115.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 02:42:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,951
            CDSs (total)                      :: 7,863
            Genes (coding)                    :: 7,657
            CDSs (with protein)               :: 7,657
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 4, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 4, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 87 of 206
            Pseudo Genes (incomplete)         :: 170 of 206
            Pseudo Genes (internal stop)      :: 23 of 206
            Pseudo Genes (multiple problems)  :: 65 of 206
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:37:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2347677
            Orig. end                         :: 2358082
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NDXL01000003.1.
            Bacteria and source DNA available from BCRC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="8"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            331..1035
                     /locus_tag="B7024_RS39330"
     CDS             331..1035
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091831.1"
                     /locus_tag="B7024_RS39330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_094795484.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYIGKARFEAGLAAFAHRDAVEVVHRSFELDA
                     DAPAATDIPILDMLATKYGVTREQAEAMEAKVAEAAAGEGLGYRSDRIHGNTFDLHRLL
                     HLAKEHGVQDALLQAFYRANFAEARPLGDAAVLSEIATGAGLPADAVARVLADPDAYAD
                     AVRADEREAAQLGAGGVPFFVIDRRYGISGGQPADVFRQALERAHAEGVVQILPTGDAE
                     VCGDDGCAVPGK"
     gene            1166..2257
                     /locus_tag="B7024_RS39335"
     CDS             1166..2257
                     /NRPS_PKS="Domain: Aminotran_5 (17-357). E-value: 5.2e-17.
                     Score: 53.7. Matches aSDomain:
                     nrpspksdomains_B7024_RS39335_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003985687.1"
                     /locus_tag="B7024_RS39335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_094795694.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.6e-10, bitscore:
                     37.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQRRAPLGGAEFAPETTYLNSASSGLLPMRTRTALHAAVDESASY
                     GTMGRDYFGPAAASRAAFARLLDIPAERVAVGSSVAVQSAFVAGTLPPGAEVLVPEGDF
                     SSLVNPFAAHPGVQLRTVPLAELAGAVRPDTALVAFSAVQSLDGAIADLAAVRAAARAH
                     GARTYLDITQAAGWLPLRADDFDFVVCGAFKWLLCPRGTTFMAFGGNGTGGGHGDRDAA
                     GAWPAALHAGWVAGEEPGESSYGPVLRPATTARRFDEPHAHYSYLGAAQSLALFEELGV
                     TTVHAHNTALADHYRAGLTARGLTPRPAPGSAIVSTPGLADAERRLTEAGVRISVRGGL
                     LRASFHLYNSTADVERLLALLDG"
     aSDomain        1217..2236
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B7024_RS39335_Aminotran_5.1"
                     /evalue="5.20E-17"
                     /label="B7024_RS39335_Aminotran_5.1"
                     /locus_tag="B7024_RS39335"
                     /protein_end="357"
                     /protein_start="17"
                     /score="53.7"
                     /tool="antismash"
                     /translation="YLNSASSGLLPMRTRTALHAAVDESASYGTMGRDYFGPAAASRAA
                     FARLLDIPAERVAVGSSVAVQSAFVAGTLPPGAEVLVPEGDFSSLVNPFAAHPGVQLRT
                     VPLAELAGAVRPDTALVAFSAVQSLDGAIADLAAVRAAARAHGARTYLDITQAAGWLPL
                     RADDFDFVVCGAFKWLLCPRGTTFMAFGGNGTGGGHGDRDAAGAWPAALHAGWVAGEEP
                     GESSYGPVLRPATTARRFDEPHAHYSYLGAAQSLALFEELGVTTVHAHNTALADHYRAG
                     LTARGLTPRPAPGSAIVSTPGLADAERRLTEAGVRISVRGGLLRASFHLYNSTADVERL
                     "
     gene            complement(2518..3837)
                     /locus_tag="B7024_RS39340"
     CDS             complement(2518..3837)
                     /NRPS_PKS="Domain: Abhydrolase_1 (47-194). E-value:
                     1.4e-17. Score: 56.1. Matches aSDomain:
                     nrpspksdomains_B7024_RS39340_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145299.1"
                     /locus_tag="B7024_RS39340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_094795485.1"
                     /sec_met_domain="PF00561 (E-value: 1.1e-17, bitscore: 61.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVQRLPGVVLTDHVLEVPLDHDEPGGERIEVYGREVVAAGRERA
                     ELPWLVYLQGGPGCPAPRPLGRESWLSRALDDYRVLLLDQRGTGRSTPANRQTLPLRGG
                     PGEQAAYLAHFRADAIVRDAETFRRRLAGDDTPWSVLGQSFGGFCTVTYLSQAPEGLRE
                     AYVTGGLPGTDASAADVYRAAYPRVARKNAAHYARFPEDVPAVRRIAAHLRAHEVRLPG
                     GGLLTAEAFQALGLMLGSGNGSPTLHHLINEAWVTGPAGPELADAFLHAVQGHLSHATT
                     PLFALLHETIYGQRSVDPGPTGWAAQRVRDGLETDAAFDVDTALAGDGPLYFTGEMMYP
                     WLFDTDPALRPLKETAQALAERTDWPDLYDTERLAANEVPAAAAVYFDDMYVDTTHSLR
                     TAERIRGLRTWVTNEWEHDGLRVSDGAVLDRLIRTVRGEL"
     aSDomain        complement(3256..3696)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B7024_RS39340_Abhydrolase_1.1"
                     /evalue="1.40E-17"
                     /label="B7024_RS39340_Abhydrolase_1.1"
                     /locus_tag="B7024_RS39340"
                     /protein_end="194"
                     /protein_start="47"
                     /score="56.1"
                     /tool="antismash"
                     /translation="PWLVYLQGGPGCPAPRPLGRESWLSRALDDYRVLLLDQRGTGRST
                     PANRQTLPLRGGPGEQAAYLAHFRADAIVRDAETFRRRLAGDDTPWSVLGQSFGGFCTV
                     TYLSQAPEGLREAYVTGGLPGTDASAADVYRAAYPRVARKNAA"
     gene            complement(4010..4909)
                     /gene="thpD"
                     /locus_tag="B7024_RS39345"
     CDS             complement(4010..4909)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="B7024_RS39345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_094795486.1"
                     /sec_met_domain="PhyH (E-value: 1.2e-36, bitscore: 123.9,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADRYPSRGATETITPREDPVVWSPPGSAGPFRPTELS
                     DFARDGFSAVGDLLTADEVAVYRAELDRLVLDPATRADPRSIIEPKTGSVRSVFEVHRI
                     SEVFAGLARDPRVAGRARQILGSDVYIHQSRINVKPGFGASGFYWHSDFETWHAEDGLA
                     RMRTVSVSIALTENLDTNGGLMIMPGSHHDFVGCAGETPKDNYKRSLQMQDAGIPSDET
                     LTKMADRHGIRLFTGRAGSATWFDCNAMHGSGDNITPYPRSNVFLVFNSVENTAVEPFA
                     APIRRPEYIGARDFTPVR"
     gene            complement(5001..5405)
                     /locus_tag="B7024_RS39350"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643612.1"
                     /locus_tag="B7024_RS39350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_052854565.1"
                     /sec_met_domain="ectoine_synt (E-value: 5.4e-54, bitscore:
                     178.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVRAASGTWESKRIVLAKERVGFSLHETILY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKHWISPGTMYLLNGHEKHTMRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPETV"
     gene            complement(5508..6776)
                     /gene="ectB"
                     /locus_tag="B7024_RS39355"
     CDS             complement(5508..6776)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 1.9e-74.
                     Score: 242.6. Matches aSDomain:
                     nrpspksdomains_B7024_RS39355_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="B7024_RS39355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_052854563.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4.9e-79, bitscore:
                     262.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTVFDRAQGSRIFDEDGHVY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMSTTAKRAFLESFQNIILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYLDGQIPDFLWFERLLEDQGSGLNQPAAVIVETVQGEGGINVARA
                     EWLRALAALCERRDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPLA
                     LTLFKPELDVWEPGEHNGTFRGNNPAFVTAAATLDAYWADGQMEKQTLARGEIVESHLK
                     AIVEEHPDAISEYRGRGLVWGLECVDKSRANTIAKRAFELGLLIETSGPESEVVKLLPA
                     LTTTPEELDEGLRTLARAVRDCV"
     aSDomain        complement(5682..6692)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B7024_RS39355_Aminotran_3.1"
                     /evalue="1.90E-74"
                     /label="B7024_RS39355_Aminotran_3.1"
                     /locus_tag="B7024_RS39355"
                     /protein_end="365"
                     /protein_start="28"
                     /score="242.6"
                     /tool="antismash"
                     /translation="FDRAQGSRIFDEDGHVYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMSTTAKRAFLESFQNIILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYLDGQIPDFLWFER
                     LLEDQGSGLNQPAAVIVETVQGEGGINVARAEWLRALAALCERRDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPLALTLFKPELDVWEPGEHNGTFRGNNPAFV
                     TAAATLDAYWADGQMEKQTLARGEIVESHLKAIVEEHPDAISEYRGRGLVWGLECV"
     gene            complement(6928..7494)
                     /gene="ectA"
                     /locus_tag="B7024_RS39360"
     CDS             complement(6928..7494)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536877.1"
                     /locus_tag="B7024_RS39360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_172451276.1"
                     /transl_table=11
                     /translation="MTAAQADHAGARTDFRELPEGFKLDTPRVADGAAIWRIARDSRTL
                     DLNSSYSYLLWCRDFAATSVVARDAAGEPAAFVTGYRRPEHPETLVVWQVAVDDAHRGR
                     GLAAALLDGLTARAVRDWGVRRVETTVTPHNDASNRLFASFAERHGVPLRREVLFDAEL
                     FPGQGHEPEVLHLIGPFALPPGPGH"
     misc_feature    complement(7494..7508)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.04"
                     /tool="antismash"
     misc_feature    7504..7519
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.79"
                     /tool="antismash"
     misc_feature    complement(7650..7665)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7660..7675
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7660..7677)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.47"
                     /tool="antismash"
     gene            8038..9129
                     /locus_tag="B7024_RS39365"
     CDS             8038..9129
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606343.1"
                     /locus_tag="B7024_RS39365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amidohydrolase family protein"
                     /protein_id="WP_172451327.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 6.6e-15, bitscore:
                     52.0, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDGAVLHLKGRVLVGPEDVRDELWVVGGRVTYDRPVAAREVTTV
                     QGWVLPGLVDAHCHVGLDADGPVDDPTSEKQALTERDAGALLLRDAGSPSDTRWIDDRE
                     DLPRIIRAGRHIARTRRYIRNYAHEIEPADLVAYVAQEARRGDGWVKLVGDWIDRSTGD
                     LEACWPRGAVAAAIAEAHRLGARVTAHCFAEDTLAPLVEAGIDCIEHATGLTEETIPLF
                     AERGVAIVPTLVNIATFPQLASGGEAKFPRWADHMRRLHERRYDTVRAAYDAGVPVFAG
                     TDAGGSLAHGLVAQEVAELRKAGLPAVAALSATTWGARDWLGRPGLTEGAPADLVVYEA
                     DPRQDVRVLAAPRRVVLRGRVIG"
     gene            complement(9278..10213)
                     /locus_tag="B7024_RS39370"
     CDS             complement(9278..10213)
                     /GO_function="GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986841.1"
                     /locus_tag="B7024_RS39370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="WP_094795695.1"
                     /transl_table=11
                     /translation="MPASDRAARRAVTVFPLLVIAAGAIGLLWPAHFTGWAPAVPWLLG
                     VVMFSMGLTLTLPDFTAVAKRPWAVGLGLVAHYVIMPGLGWLIATVLHLPPQLAAGVIL
                     VGCAPSGTASNVVTFLARGDVALSVSVATVSTVLAPLITPPLTLLLAGQFLDVDAGVMV
                     TDILKTVLLPVAAGLLVRVVAGRYVDKVLGALPWLSAVTIAAIVLAVVAGSAALIKDAA
                     ALVLLAVVLHNGLGLALGYGAGKLARLGRPASRAMAFEVGMQNSGLAASLATAHFSPAA
                     ALPAAVFSVWHNVSGAVVAAWMARTERRTR"
ORIGIN
        1 gacggttcgt ccaccgtccg gggcagtgtc atgcggcagc cccgaactct gcagcggtct
       61 tgaggccgtg gcacccgcgg cacagcacca gagcgttccc gtccacgccg tccatgcctt
      121 ccacgactca ggcgactcgc ggcccccgga tccgggtgag tgcagtgtga tcaggccatc
      181 gcgttccgtg gtcgtccgtc cgtcgaagag gaccacctcc tgagtctgcg gcgtcgcggg
      241 ggaccggcag aggcatgaac agctggccgg aatagagcgg tctcccgcgt ggttggacag
      301 gggcacattc accccgagga aagtcagctc atgcgcgtcg aaatctggtc ggacatcgct
      361 tgcccctggt gctacatagg caaggcccgc ttcgaagcgg ggctggccgc gttcgcgcac
      421 cgtgatgcgg tcgaggtggt gcaccgctcg ttcgagctgg acgcggacgc gccggccgcc
      481 accgacatac cgattctgga catgctcgcc acgaagtacg gcgtgacccg tgagcaggcc
      541 gaggccatgg aggccaaggt tgccgaggcg gcggccggtg aggggctcgg ctaccgctcc
      601 gaccggatcc acggcaacac cttcgacctg caccggctgt tgcacctggc caaggagcac
      661 ggcgtgcagg acgcgctgct ccaagccttc taccgggcca acttcgccga ggcccggccg
      721 ctgggcgacg ccgccgttct cagtgagatc gccaccggcg cgggactgcc cgccgacgcc
      781 gtcgcgcggg tgctggccga cccggacgcg tacgcggacg cggtgcgggc cgacgagcgt
      841 gaggccgcgc agctgggcgc gggcggggtg ccgttcttcg tcatcgaccg gcgctacggc
      901 atctccggcg gccagcccgc cgacgtcttc cgccaggcgc tggagcgcgc ccacgcggag
      961 ggcgtcgtcc agatcctgcc gaccggcgac gccgaggtct gcggggacga cggctgcgcc
     1021 gtacccggga agtgaccgtg cccgggaagt gaccgcgggc cggcccgccc gcatccgctc
     1081 cgaccggagc ggatgcggaa ggcttgcggg ccggccgggc ggagatgtca tggacggggc
     1141 accgcgcgtc gagcagagtg ggggcatgca gcgacgcgcg ccactgggcg gggcggaatt
     1201 cgcgcccgag acgacctatc tgaactccgc ttccagcgga ctgctcccga tgcgcaccag
     1261 gaccgcactg cacgcggcgg tcgacgagtc cgcctcctac ggcaccatgg gccgcgacta
     1321 cttcggcccg gccgcggcct cccgggccgc cttcgcccgg ctgctggaca tccccgcgga
     1381 gcgcgtcgcc gtcggcagct ccgtcgcggt gcagtccgcg ttcgtcgccg gcacgctccc
     1441 gccgggcgcc gaagtgctgg ttcccgaggg cgatttcagc tctctggtca atccgttcgc
     1501 cgcgcacccc ggcgtccagc tgcgtaccgt gccgctggcg gagctcgccg gggcggtccg
     1561 gccggacacc gcgctcgtcg ccttcagcgc agtgcagtcg ctggacggtg cgatcgcgga
     1621 cctggcggcg gtccgggccg cggcgcgggc gcacggcgcg cgcacctacc tcgacatcac
     1681 ccaggccgcg ggctggctgc cgctgcgcgc ggacgacttc gacttcgtcg tctgcggggc
     1741 cttcaaatgg ctgctgtgcc cgcgcggcac caccttcatg gcgttcggcg ggaacgggac
     1801 cggcggcggc cacggggacc gggacgccgc cggggcgtgg ccggccgcgc tgcacgcggg
     1861 ctgggtggcg ggcgaggagc cgggtgagtc cagctacggt ccggtcctgc ggccggccac
     1921 caccgcccgg cgcttcgacg agccgcacgc ccactactcc tacctcggcg ccgcgcagtc
     1981 cctggcgctc ttcgaggaac tcggcgtcac caccgtccac gcccacaaca ccgcgctggc
     2041 cgaccactac cgcgccggcc tcaccgcccg cggtctcacc ccgcgcccgg cgcccggctc
     2101 ggcgatcgtc tccacccccg ggctcgcgga cgcggagcga cggctgaccg aggcgggcgt
     2161 acggatctcg gtgcgcggcg gactgctccg ggcgtcgttc catctctaca actccaccgc
     2221 ggacgtggag cggctgctgg cactgctcga cggctgactt ccgtgcggcg tccggtgggg
     2281 cggggctctc ctcgccccac tggacggctg ccccaaggga gtcggccggc gcggactgcg
     2341 ccgggaatcc ccgcctccga ccccgtagca ctccccggaa acgacgaagc ccggaacgaa
     2401 gctgagcgag gtccggcgca tgcggcgggt cccgcttccg gccggccggc tggccctggg
     2461 tggggatgcg acggtcaccc cgccccaccc ccgcacctca ggcgcccccg cgccccctca
     2521 cagctctccg cgtaccgttc tgatcagccg gtcgagtacc gcgccgtcgc tcacgcggag
     2581 tccgtcgtgc tcccactcgt tggtcaccca cgtccgcagt ccgcggatgc gctccgcggt
     2641 tcgcagggag tgggtggtgt cgacgtacat atcgtcgaag tagaccgcgg cggcggccgg
     2701 tacctcgttg gcggccagtc gttcggtgtc gtacaggtcg ggccagtccg tccgctcggc
     2761 cagtgcctgt gcggtctcct tcagggggcg cagcgccggg tcggtgtcga agagccaggg
     2821 gtacatcatc tcgccggtga aatacagcgg tccgtcgccg gccagtgcgg tgtccacatc
     2881 gaaggcggcg tccgtttcga ggccgtcccg cacccgttgc gccgcccagc cggtcgggcc
     2941 agggtccacc gaccgctgtc cgtagatcgt ctcgtgcagc agcgcgaaga gcggggtggt
     3001 ggcgtgcgag aggtgtccct ggacggcgtg caggaacgcg tcggcgagtt cggggcccgc
     3061 ggggccggtc acccatgcct cgttgatcag gtggtgcagg gtgggcgagc cgttgccgct
     3121 gcccagcatc aggcccagcg cctggaacgc ctcggccgtc agcagtccgc cgcccggcag
     3181 ccgtacctcg tgggcgcgca gatgtgccgc gatccggcgg acggccggta cgtcctcggg
     3241 gaagcgggcg tagtgcgcgg cgttcttccg ggcgacccgc gggtacgccg cccggtagac
     3301 gtccgccgcg ctcgcgtccg tccccggcag cccgcccgtc acatacgcct cgcgcagccc
     3361 ctcgggcgcc tgcgagagat aggtgacggt gcagaaaccg ccgaagctct ggcccagcac
     3421 gctccacggg gtgtcgtcgc cggccagccg gcggcggaac gtctcggcgt cccgcacgat
     3481 cgcgtcggcg cggaagtgcg ccagatacgc ggcctgttcg ccggggccgc cgcgcagcgg
     3541 gagtgtctgc cggttggcgg gcgtcgagcg gccggtgccg cgctggtcca gcagcaggac
     3601 gcggtagtcg tccagcgccc gggacagcca ggattcgcgc ccgagcggcc gcggcgccgg
     3661 gcagccgggg ccgccctgca agtacaccag ccacggcagt tccgcccgtt cgcgcccggc
     3721 ggccaccacc tcgcggccgt acacctcgat ccgctcaccg cccggttcgt cgtggtccag
     3781 gggcacttcc agtacgtggt cggtcagcac gaccccgggc agccgctgca cactcatgac
     3841 gtctctcctc gccttcgctc caccgcgcgg cacaccggcc gcccggcccg gtgcccaccg
     3901 cggcgggtcc ggacccctgg tcaggcgtca ggctaacgac gcggaccgcc gccccgggcc
     3961 tcggggcggc ggtccgtacc gtcgacctgg tgcggcagcg gcgagcgggc taccgcaccg
     4021 gcgtgaagtc gcgggcgccg atgtactccg ggcgccggat cggcgccgcg aacggctcca
     4081 ccgcggtgtt ctccacgctg ttgaagacga ggaacacatt gctgcgcggg tacggcgtga
     4141 tgttgtcgcc ggaaccgtgc atggcgttgc agtcgaacca ggtcgccgag ccggcccggc
     4201 cggtgaacag gcggatgccg tgccggtccg ccatcttcgt cagcgtctcg tccgacggga
     4261 tgcccgcgtc ctgcatctgg agggatctct tgtagttgtc cttcggcgtc tcgcccgcgc
     4321 atcccacgaa gtcgtggtgg gagccgggca tgatcatcaa gccgccgttg gtgtcgaggt
     4381 tctcggtcag cgcgatggag acggacacgg tgcgcatccg cgccagaccg tcctccgcgt
     4441 gccaggtctc gaagtccgag tgccagtaga aaccggaggc gccgaacccg ggcttcacat
     4501 tgatccgcga ctggtggatg tagacgtccg agccgaggat ctgccgggcg cggccggcca
     4561 cgcgcgggtc gcgcgccagc ccggcgaaca cctcgctgat ccggtgcact tcgaagacgg
     4621 accgtacgct ccccgtcttc ggctcgatga tcgaacgcgg gtcggcgcgc gtcgccgggt
     4681 ccagcacgag ccggtccagt tcggcgcggt agaccgcgac ttcgtccgcc gtcagcaggt
     4741 caccgacggc gctgaagccg tcgcgggcga agtcgctcag ctcggtcggc cggaaggggc
     4801 cggccgagcc gggcggtgac cacaccaccg ggtcctcccg cggggtgatc gtctcggtgg
     4861 ccccacggga cgggtagcgg tcggcggtgc gctcgggtgc ggtggtcata tgggccttcc
     4921 tctcctctcg tacatgctct cggccaggta tccggggtgt gtcctcctcg gccgccgcac
     4981 ggggcgggcg gcaatgcgcc tcagaccgtt tcgggttcgg tgagcaacgg gtagacgccg
     5041 ttctcgtcgt ggtcctcgcg gccggtgacc ggggggttga agacacagac gcagcggaag
     5101 tcggtcttgg gccgcatggt gtgcttctca tggccgttga gcaggtacat cgtgcccggc
     5161 gagatccagt gcttctcgcc ggtctcgtcg ttggtgagct cggcctcgcc ctccacgcac
     5221 aggacggcct cgatgtggtt cgcgtaccac atcgaggtct cggttcccgc gtacaggatg
     5281 gtctcgtgca gcgagaagcc gacgcgctcc ttggcgagca cgatgcgctt gctctcccag
     5341 gtgccggagg cggccctgac gtggcggtcg gtgccttcga tgtccttgaa ggatcggacg
     5401 atcacggtga gtcggtgcct ttctgtggaa cggctgtacg ggacgggtgc ggtgggcgcg
     5461 tgcggtgaac ggggcggccg gccgggggtg ccgggtggcc gggtccgtca gacgcagtcg
     5521 cggaccgcgc gggcgagggt gcgaaggccc tcgtccagtt cctcgggggt ggtcgtcagc
     5581 gcgggcagca gcttgacgac ctcgctctcc gggccggagg tctcgatgag cagacccagc
     5641 tcgaaggcgc gcttggcgat cgtgttggcg cggctcttgt ccacgcactc caggccccac
     5701 accagtccgc ggccgcggta ctcggagatg gcgtccgggt gctcctcgac gatggccttc
     5761 agatgggact cgacgatctc gccgcgggcg agcgtctgct tctccatctg gccgtcggcc
     5821 cagtacgcgt ccagcgtggc ggcggcggtg acgaaggccg ggttgttgcc gcggaaggtg
     5881 ccgttgtgct cgcccggctc ccatacgtcc agctcgggct tgaagagggt gagcgcgagc
     5941 ggcagaccgt agccgctgat ggacttcgag acggtgacga tgtccggcac gatgcccgcc
     6001 tcctcgaagg agaagaacgc gccggtgcgg ccgcagccca tctggatgtc gtccacgatc
     6061 agcagcatgt cgcggcgctc gcacagcgcg gcgagggcgc gcagccattc ggcgcgggcg
     6121 acgttgatgc cgccctcgcc ctggacggtc tcgacgatca ccgcggcggg ctggttcaga
     6181 cccgaaccct ggtcctccag cagccgctcg aaccagagga agtccgggat ctggccgtcg
     6241 aggtagttgt cgaacggcat cggggtgccg tgcaccagcg ggataccggc gccggcccgt
     6301 ttgaaggcgt tgccggtgac ggccagcgag cccagcgaca tgccgtggaa ggcgttggtg
     6361 aaggacacga tcgactcgcg gcccttgacc ttgcgggcca gtttcagcgc ggcctcgacg
     6421 gcgttggtgc ccgtcgggcc cgggaacatg accttgtagg gcaggtcgcg cggccgcagg
     6481 atgatgttct ggaaggactc caggaacgcc cgcttggccg tggtcgacat gtccaggccg
     6541 tgggtgatgc cgtcccgctc gatgtagtcg atcagggcgc gcttgaggac cgggttgttg
     6601 tggccgtagt tgagcgatcc ggccccggcg aagaagtcga ggtagacgtg gccgtcctcg
     6661 tcgaaaatcc ggctgccctg cgcgcggtcg aagacggtcg gccagccgcg gcagtagctg
     6721 cgtacctccg actccacggt ctcgaagacg ctcagatcgg gctgggtgat ggtcacagca
     6781 tgctcctggg agatgaagtg gcgaacggag tggaagtctg cggggcgggc ggccggatgc
     6841 ggcgctctca cggggcgcgc ccttcccgtg cggcggacgc gggacccgaa cggcctaccc
     6901 ggggcggccg cgcgcccggt ccggccgtca gtgtccgggg ccgggcggaa gggcgaacgg
     6961 cccgatgagg tgcagcacct ccggctcgtg gccctgcccg gggaagagct ccgcgtcgaa
     7021 gagcacctcg cgcctgagcg gtacgccgtg gcgctcggcg aacgacgcga acagccggtt
     7081 ggaggcgtcg ttgtgggggg tgacggtggt ctcgacgcgg cggacgcccc agtcgcgtac
     7141 ggcgcgggcg gtcagtccgt ccagcagcgc ggccgccagc ccgcggccgc ggtgcgcgtc
     7201 gtcgacggcg acctgccaca cgacgagggt ctccgggtgc tcggggcggc ggtagccggt
     7261 gacgaaggcg gccggttcgc ccgccgcgtc gcgtgccacg acggaggtgg cggcgaagtc
     7321 gcgacaccac agcaggtagc tgtaggagga gttgaggtcc agggtccggg aatcgcgggc
     7381 gatgcgccag attgcggctc cgtccgccac ccgaggggtg tcgagcttga agccctcagg
     7441 caattccctg aaatcggtac gggcgcctgc atggtctgct tgtgcggcgg tcatggaaat
     7501 taaatttacc cagggaaaaa ggaaattgca tcgcgggcag aggttacgca agtcgtgttg
     7561 ctgtgttatc gcgcgggtgc gcccgagcgc gcgttgtggc tgcaagatgt acggttttga
     7621 ccaggaggtt cggggtgaaa cgggcgcgct gtggggtcgg tcacaggtcc gtaacacccg
     7681 gggaagccat ccgaattacg gtgccggagg ctagcggaat cttagcgttt gagctgcgga
     7741 aaagcgggca ggagaatacg gggagctgcg cttctgaaga attgtgaatt tgcctgcgga
     7801 aggtgccgtg gaagcgcatc gcaaggcgct tcggaaggtc ccccgaaagg cgtgcgagaa
     7861 gttgcgcgga aaggaatggc ggggaacggg agagcaaagc ggcgccgacg cccgggcggg
     7921 gcgggaaatc ggccacggaa cgggcccgcg gaaatgcgcg ggagcccgcc gcggagcccg
     7981 ccggacggcc gggtccacct ccggtggcgc gccgcatagg gtgcgtccat gatggcgatg
     8041 agtgacggtg cggtgctgca cctcaagggg agggtcctcg tcggcccgga ggatgtgcgc
     8101 gacgaactct gggtcgtcgg cggccgggtg acgtacgacc ggccggtcgc ggcgcgggag
     8161 gtcacgaccg tccaggggtg ggtgctgccg ggcctggtgg acgcccactg ccatgtgggg
     8221 ctggacgccg acggaccggt ggacgacccc accagcgaga agcaggcgtt gaccgaacgc
     8281 gacgcgggcg cgctgctgct gcgcgacgcc ggatcgccct ccgacacccg ctggatcgac
     8341 gaccgtgagg atctgccgcg gatcatccgc gcgggccggc acatcgcccg cacccgccgc
     8401 tacatccgca actacgccca tgagatcgag ccggcggacc tcgtcgccta tgtcgcccag
     8461 gaggcccggc ggggcgacgg ctgggtcaag ctcgtggggg actggatcga ccgctcgacc
     8521 ggcgacctgg aggcgtgctg gccgcgcggc gccgtcgcgg cggccatcgc cgaggcgcac
     8581 cgcctcggcg cccgggtgac cgcccactgc tttgccgagg acaccctcgc accgctcgtc
     8641 gaggccggta tcgactgcat cgagcacgcc accggcctca ccgaggagac catcccgctg
     8701 ttcgccgagc gcggtgtcgc catcgtcccc acgctcgtca acatcgcgac cttcccgcag
     8761 ctcgcgtcgg gcggcgaggc gaagttcccg cgctgggcgg accatatgcg acggctgcac
     8821 gagcgccgct acgacacggt gcgggcggcg tacgacgcgg gcgtgcccgt cttcgccggt
     8881 acggacgcgg gcggctcgct cgcgcacggt ctggtcgccc aggaagtcgc cgagctgcgg
     8941 aaggccggtc tgccggccgt cgccgcgctc tccgcgacga cctggggcgc ccgggactgg
     9001 ctgggccggc cgggcctgac cgagggcgcc cccgccgacc tggtggtcta cgaggccgat
     9061 ccgcgccagg acgtacgggt gctggccgca ccccgccggg tggtgctgcg cgggcgggtg
     9121 atcggctgag gggccggtgg cggggcgggc ccgtggcggt tcatccggtg gccggaccgg
     9181 cgcggcgctc ggaccggtga ccgccggtcc ggccaccggg cttcgccctg ccgcgtcccg
     9241 cccgccccgt cccgcccgcc cggggcgacc cgcggtgcta cctggtgcgg cgctcggtgc
     9301 gggccatcca ggccgcgacc accgcgcccg agacgttgtg ccagacggag aagaccgccg
     9361 cgggcagcgc cgccgccgga ctgaagtgcg cggtggccag cgacgcggcc aggccggagt
     9421 tctgcatgcc gacctcgaag gccatcgccc ggctcgcggg gcggcccagc cgggccagct
     9481 tccccgcccc gtaaccgagc gccagcccga gcccgttgtg cagcaccacc gcgagcagca
     9541 ccagcgccgc cgcgtccttg atcagcgcgg cgctgcccgc gaccacggcc agcacgatgg
     9601 ccgcgatggt cacggcggag agccacggca gcgcgccgag caccttgtcc acgtagcggc
     9661 ccgcgacgac ccggaccagc agcccggccg cgaccggcag cagcaccgtc ttgaggatgt
     9721 cggtcaccat gaccccggcg tccacgtcca ggaactgccc ggcgagcagc agcgtcagcg
     9781 ggggagtgat gagcggggcg agcaccgtgg agacggtggc gacggagacc gagagcgcga
     9841 catcgccgcg ggccaggaac gtgacgacgt tggacgcggt gccgctgggc gcgcagccga
     9901 cgaggatcac ccccgccgcg agctgcggcg gcagatgcag cacggtcgcg atcagccagc
     9961 ccagccccgg catgatcacg taatgcgcga ccaggcccag gcccaccgcc cagggccgct
    10021 tggcgaccgc ggtgaagtcc ggcagggtca gcgtcagccc catcgagaac atcaccacgc
    10081 cgagcagcca cggcaccgcg ggcgcccagc cggtgaagtg cgcgggccac agcagcccga
    10141 tcgcgccggc ggcgatcacc agcagcggga agaccgttac cgcccggcgc gccgcccggt
    10201 cggaggcggg caggtcgccc gggggtgcgg gccgcgccgg tgtcgcgggg ccggtggccg
    10261 gatccggtct ctggttcgtc tgctcggttg acacggcggc accttatccg tcatgtccgt
    10321 gtacgagaca acggattccg gatcgtggac accgtccggc gtcagcgccg cgcctcggcg
    10381 tccgccgcgg acgcccgcag cagcg
//
