LOCUS       NZ_NDXL01000001        12979 bp    DNA     linear   CON 27-APR-2024
DEFINITION  Streptomyces kasugaensis strain BCRC 12349 scaffold1, whole genome
            shotgun sequence.
ACCESSION   NZ_NDXL01000001
VERSION     NZ_NDXL01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces kasugaensis
  ORGANISM  Streptomyces kasugaensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Comparative genome analysis for kasugamycin overproduction
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Comparative genome analysis for kasugamycin overproduction
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030,
            China
REFERENCE   4  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. May-2015
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 188.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002261115.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 02:42:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,951
            CDSs (total)                      :: 7,863
            Genes (coding)                    :: 7,657
            CDSs (with protein)               :: 7,657
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 4, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 4, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 87 of 206
            Pseudo Genes (incomplete)         :: 170 of 206
            Pseudo Genes (internal stop)      :: 23 of 206
            Pseudo Genes (multiple problems)  :: 65 of 206
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:37:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4719930
            Orig. end                         :: 4732909
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NDXL01000001.1.
            Bacteria and source DNA available from BCRC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12979
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="29"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12979
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12979
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7979](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7979)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    complement(642..655)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.96"
                     /tool="antismash"
     misc_feature    complement(717..733)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.0"
                     /tool="antismash"
     misc_feature    721..739
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.5"
                     /tool="antismash"
     gene            873..1946
                     /locus_tag="B7024_RS20120"
     CDS             873..1946
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547330.1"
                     /locus_tag="B7024_RS20120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="WP_052853417.1"
                     /transl_table=11
                     /translation="MTRAEWGRVAGMAGFILALHVIGWFTLVAIVAPEHYSLGTKSFGI
                     GIGVTAYTLGMRHAFDADHIAAIDNTTRKLMNDGQRPLSVGFWFSLGHSSIVFGLAFLL
                     SLGVKALAGPVRDDSSQLHDVTGLIGTTVSGVFLYVIAAINIVILVGIWQVFRRMRAGD
                     FDEAALEEHLNNRGFMNRLLGRVMKSITKPWQMYPLGLLFGLGFDTATEIALLVLAGSG
                     AASGLPWYAILCLPVLFAAGMSLLDTIDGSFMNFAYGWAFSKPVRKVYYNLTITGLSVA
                     VALIIGTVELLGLLAEKFDLHGAFWDWISGLDLNLIGFVIVGIFFATWIIAMAVWKFGR
                     IEEKWTANVRPADRSAE"
     gene            complement(2014..2952)
                     /locus_tag="B7024_RS20125"
     CDS             complement(2014..2952)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1158: ornithine cyclodeaminase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537613.1"
                     /locus_tag="B7024_RS20125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ornithine cyclodeaminase"
                     /protein_id="WP_094792801.1"
                     /transl_table=11
                     /translation="MTAPRTIDGAALRRALPMAAAIGALRAALRDGTDPESDPPRTVVP
                     VEHGQLLLMPAHRRRSAGVKIATVAPANPAAGLARVQGRYLLFDAPTLSPLALLDGIEL
                     TTVRTAAVSALAVDLLAGPEAGHLVLFGTGPQARGHLDALRVVRPLTRVTVVGRTPAHV
                     ERFAETARRDTGLPVAPGDPSAVATADLVACCTTARTPLFDGALLAPHATVTAVGSHEP
                     DAREVDSGTVRRCTVVVESRAAAVREAGDLIPELAAGRLDRAALIPLADLATGAATVDR
                     TRPRLFKSVGMAWEDLVVAGAAYEADAAEDA"
     gene            complement(2949..3950)
                     /locus_tag="B7024_RS20130"
     CDS             complement(2949..3950)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763263.1"
                     /locus_tag="B7024_RS20130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="proline racemase family protein"
                     /protein_id="WP_052853260.1"
                     /transl_table=11
                     /translation="MRSNRIFHAVDSHTEGMPTRVITGGVGVIPGDTMFARRRYFIDHL
                     DHIRTFLMNEPRGHAAMSGAILQPPTRPDADFGVLYIEVSGCLPMCGHGTIGVATVLVE
                     TGMVEVTEPTTTIRLDTPAGLVVAEVRVVDGAAKSVTLRNVPSFALGLDRTVRVEPWGD
                     IPYDLAFGGNFYAIVPLEALGIPFERAEKQRILDAGLAVMAAVDEQDRPVHPGNPEING
                     CHHVYLAAPGSDARRSRHAMVIHPGWFDRSPCGTGTSARMAQLHARGELTLDTDFDNES
                     FIGTHFTGRLVEEGRLGDLTTVTPTITGRAWITGTAQFHRDPEDPFPAGFVL"
     gene            complement(3955..4869)
                     /locus_tag="B7024_RS20135"
     CDS             complement(3955..4869)
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720319.1"
                     /locus_tag="B7024_RS20135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_094792802.1"
                     /transl_table=11
                     /translation="MNAQAQPWHGVLVATALPWRESRTGELTADFDGYAEHLGWLAENG
                     CHGVTPNGSLGEYQTLTPEERARVVTTAVEVAADTPGFTVMPGVAAYGADEARRWAEHA
                     GAAGARAVMLLPPNAYRADRRAVVAHYREVARAGLPVVAYNNPFDTKVDLVPELLAELY
                     GEGLIVAVKEFSGDVRRAYRIAELAPGLDVLCGADDVALELAAAGSPGWVSGFTNAFPR
                     ACVELWQAASAGDLSTALPLYRTLHPLLRWDSRIEFVQAIKLSMDLAGRYGGPCRQPRL
                     PLAPEHEESVRQATGKALAEGLR"
     misc_feature    complement(4899..4919)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 22.13"
                     /tool="antismash"
     misc_feature    4910..4920
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 19.14"
                     /tool="antismash"
     misc_feature    complement(4914..4930)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.3"
                     /tool="antismash"
     misc_feature    complement(4916..4926)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 19.14"
                     /tool="antismash"
     misc_feature    complement(4979..4994)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.28"
                     /tool="antismash"
     gene            complement(5001..6488)
                     /locus_tag="B7024_RS20140"
     CDS             complement(5001..6488)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378675.1"
                     /locus_tag="B7024_RS20140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_094792803.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 3.4e-19, bitscore:
                     67.2, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 5.5e-08, bitscore:
                     31.1, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTSRADGPYDLAVLGAGPAGLAAAVTAAAAGARVALVDAGPRVG
                     GQYWRHRPGDTGAAHHGWAAFQRLRHGLAAHAGRLHHLPGHAVWHTGRTAGGFRTHTLH
                     DGTEQVVESTAVVVATDAYDHQLPFPGWTLPGVFTAGGAQALLKGHGVVAGRRIAVAGT
                     GPFLPAVAAGLAEAGARVVGVSEAGLPASFVRQPLTVAANPAKLAEGAGFLRTLLRHRV
                     PYRTRTAVVAAHGEDEVTGVTVARVDARWRVVPGSERRLDCDTLAVGYGFTPQIEIPLQ
                     LGCATRRDIDGSLVATADARQRGSVPGVYLAGEVCGVGGAALSLTEGELAGLHAAQAVG
                     AAVADPVVVRRLVRRRTAQRQFAAALHRAFPVRPGWLGWLRPETVVCRCEEVTVRTATE
                     AVTELGATDPRAVKLYARPGMGLCQGRVCGYATACLVAARSGREATAEELRGMAGRPIA
                     QPLPLGILAAGDPGADPESGSGIVPEAGTDESSMPAR"
     gene            complement(6469..6804)
                     /locus_tag="B7024_RS43395"
     CDS             complement(6469..6804)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720317.1"
                     /locus_tag="B7024_RS43395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_094792804.1"
                     /sec_met_domain="Fer2_4 (E-value: 2.9e-24, bitscore: 82.9,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTDTRAGAGGPAAPRRPSFPFPFPFRFDELTIEAEPGQSIAAAL
                     LAAGHRSWRRTRVAGDGRGMFCGIGVCFDCLVTVNGRPNQRACLVEAAPGDLVRTQEGA
                     GRDDLAC"
     gene            complement(6801..7979)
                     /locus_tag="B7024_RS20150"
     CDS             complement(6801..7979)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018565798.1"
                     /locus_tag="B7024_RS20150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_094792805.1"
                     /sec_met_domain="DAO (E-value: 5e-63, bitscore: 211.8,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDRSTNRSPDVVVIGAGMVGAACAYHCTSAGLRVTVVERGAIGGA
                     TTAACEGNILLSDKEPGAELELALLSSRLWARTAQALGAERLEYQEKGGVMVAASPATA
                     DGLRKLTAVQRAAGIDAVDVSRDEVTGLEPNITPEVAAGAYYPQDCQVQPVLATARLLR
                     HAREHGATVLPGTAVTGFVRDSRGAVTGVRTDSATAPLIPAGRVVNAAGTWGGELAGLA
                     GVPLPVLPRKGFILVTEPLPRVVRHKVYTAEYVANVASGDAGLQTSVVVEGTRAGTVLI
                     GASRERVGFDRSVSLPVLRRLAAQAIGVFPFLAEVALLRSYLGFRPYCPDHLPVIGADP
                     RAPGLLHACGHEGAGIGLSAATGHLIAQVITGAAPDLDLTPFRPERFEETTV"
     misc_feature    7985..7995
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     misc_feature    complement(7991..8001)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     gene            8167..8445
                     /locus_tag="B7024_RS20155"
     CDS             8167..8445
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979891.1"
                     /locus_tag="B7024_RS20155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_052853264.1"
                     /transl_table=11
                     /translation="MSLMTDGKAPGPVRFYLACDRGKCTERISFDLVISEPRPDEEADF
                     LGHILHEAHHAAPLIADLGWVFIQGGEGYWCPACAGPTRDTGTPDPS"
     misc_feature    complement(8528..8547)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: medium, score: 25.53"
                     /tool="antismash"
     gene            8873..10204
                     /locus_tag="B7024_RS20160"
     CDS             8873..10204
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /GO_process="GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1117:
                     sodium/hydrogen exchanger"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /locus_tag="B7024_RS20160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cation:proton antiporter"
                     /protein_id="WP_172450797.1"
                     /transl_table=11
                     /translation="MAGSSLEAIVLVDIAVVLLIGVALAPLRTRLRQPVVVGEIAVGVL
                     LGPSVLGLLPGNLPAKVFPVEVRSHLSVIAQVGIVLFMFVAGWELEFHLLRGRVRSVLA
                     ITAASLTVPCALASAVAFALFAGHSSLAPAHISPPLFMLFMGVVLSVSSLSVMIRILGE
                     NGLQGTTAGAMATASGAITDLVAWFAFVCLITAAQGLSTGNLLLTLGRVTAYSLAMGFV
                     VRPVLRALLRRVHPQGGHSLLLIFAVGSGVFVSAWFATCIGIHAVIGAFTFGLVMPRDL
                     DPAVRRAIATPLHHVGALLIPVFFGLTGLSVDMGALGWGGALALTAFFVVSLGGKFTGT
                     AVTARLFHLPWREAATVGVLVNSKGLGEVLMLTMGREAGLINDQLFSALFLTALLMTAS
                     VNPLVRRLTPKAGSTDPGAAVPAQQQGPHAAPELSDDHTTRGPT"
     gene            complement(10278..11072)
                     /locus_tag="B7024_RS20165"
     CDS             complement(10278..11072)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018541032.1"
                     /locus_tag="B7024_RS20165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="WP_094792807.1"
                     /transl_table=11
                     /translation="MRIALCQMTASTDPEENLAAVQDHVRRAAHAGARLAVLPEAAMVR
                     FGVPLGPVAEPLDGPWAEGVRAVARETGVTVVAGMFTPAPDGRVVNTLLATGPGVEDSY
                     DKIHLYDAFGFRESDSVAPGTRVVTIDVDGVRIGLATCYDLRFPELFRAHADAGATVSV
                     LPASWGAGPGKLAQWELLVRARALDATVWLAAVGHAAPDPLADPELPTKAPTGIGHSLL
                     AAPDGTVRARLGAAPDLLTADVDLAETDRVRRAVAVLANRRL"
     misc_feature    complement(12759..12773)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.44"
                     /tool="antismash"
ORIGIN
        1 gatcttctcg tcctgggcgt actcgacgcc cgccgggttc acgatggttg cgacgacctt
       61 gacgtcatcg ccctgctgga agaccgcccc cggctgcggg ctcagcgtga gccgctcgac
      121 gtgccgcgcc gcgtcacaga tcagcttgtc gttggggagg gtggcggtgg ctatggacgg
      181 cgcactgccg tccggtgtgt ccaggttcgt cgcggtggtc gtcacgtgga catccgaacc
      241 cgggtcgcag gagtaggtga gatacgcctt gatgaccggc gcgtttctcg tatcgagggc
      301 gattctgtcg acggtcatgg tgttggcctt ggccgcggcc ggggaggcga tggcgaccgc
      361 gcccgcggtc agcatgaggg tggcgatggc ggtaccggtg acccgatggg tgaggcggga
      421 cgacagcatg gatggctccc tcttccttct gtctcgatct gtctcgaact gtttcggttt
      481 tcttctgccg cgatctgttc ggtcactctt cttggcgtga cggcggtggc cgtcgcgacc
      541 gccgtgatcg ctttctgccg gtgtggtgcc tcgtgggcga gttacggctg gatcatcaca
      601 ggggttgccg atccccgtcc agagctttgg ccggaaggtg cttgatcttg ctcatcgcgc
      661 atcgtggctg gtgaggcggg gtgagggcga gatgcgggag ctgtccgagg gctttactgc
      721 aagttggttg cagttgacaa ttgatggcaa cagcatggga cgggctcgat caaccggcag
      781 aaagcagtgt ccccatggcc accaccacgt cctcctcacc caccccgggc gccggcgcca
      841 caccgtcgcg cagacagcgc atcgcgcgct cgatgacccg ggcggagtgg gggagggtgg
      901 ccggaatggc cggtttcatt ctcgccctgc atgtcatcgg ctggttcacg ctggtggcga
      961 tcgtggcacc ggagcattac agcctgggca ccaaatcgtt cggtatcggt atcggtgtca
     1021 ccgcctacac cctgggcatg cggcacgcct tcgacgccga ccacatcgcc gccatcgaca
     1081 acaccacccg caagctgatg aacgacggcc agcgcccgct gtccgtcgga ttctggttct
     1141 ccctggggca ttcgagcatc gtcttcggac tggccttcct gctctcgctc ggcgtcaagg
     1201 ccctcgccgg gccggtacgg gacgacagct ctcagctgca cgacgtcacc ggcctgatcg
     1261 gcaccaccgt ctccggcgtc ttcctctacg tcatcgcggc gatcaacatc gtcatcctcg
     1321 tcgggatctg gcaggtcttc cggcggatgc gcgccggtga tttcgacgag gcggcgctgg
     1381 aggagcacct caacaaccgg ggcttcatga accgcctgct cggccgcgtc atgaagtcca
     1441 tcaccaagcc gtggcagatg tatccgctgg gcctgctgtt cggcctcggc ttcgacaccg
     1501 ccaccgagat cgccctgctc gtcctcgcgg gctccggtgc cgcgtccgga ctgccctggt
     1561 acgccattct gtgcctgccc gtgctgttcg ccgcggggat gagcctgctc gacaccatcg
     1621 acggttcgtt catgaacttc gcctacggct gggccttctc caagccggtc cgcaaggtct
     1681 actacaacct caccatcacg ggcctgtcgg tcgccgtcgc cctgatcatc ggcaccgtcg
     1741 aactgctcgg gctgctggcc gagaagttcg acctgcacgg ggccttctgg gactggatct
     1801 ccggtctgga cctcaacctg atcgggttcg tcatcgtggg catcttcttc gcgacctgga
     1861 tcatcgcgat ggcggtgtgg aagttcggcc gcatcgagga gaagtggacg gcgaacgtcc
     1921 gccccgccga ccgatccgcg gagtagccgg ggcggcgggg tgaggcggct cgttctcccc
     1981 tcagcgggag gggggacgag ccgcctcacc cttttacgcg tcttctgccg cgtcggcctc
     2041 gtacgcggcg ccggccacca ccaggtcctc ccacgccatg ccgacgctct tgaagagccg
     2101 ggggcgggtc cggtccacgg tcgcggcccc ggtggccagg tcggcgagcg ggatcagcgc
     2161 ggcccggtcg aggcggccgg cggccagctc cgggatgaga tcaccggctt cccgtacggc
     2221 ggcggcccgg gactcgacca cgacggtgca gcggcggacg gtgccgctgt cgacctcgcg
     2281 ggcgtcgggt tcgtgggatc cgacggccgt cacggtggcg tgcggggcga gcagggcgcc
     2341 gtcgaagagc ggggtcctcg cggtggtgca gcaggcgacg agatcggcgg tcgcgacggc
     2401 ggacggatca ccgggtgcga cggggagccc ggtgtcccgg cgggcggttt cggcgaaccg
     2461 ctcgacatgc gcgggcgtgc gtcccacgac ggtgacccgg gtgagcgggc ggaccacccg
     2521 cagcgcgtcc aggtggccgc gcgcctgggg gccggtgccg aacagcacca gatggccggc
     2581 ttcggggccg gcgagcaggt ccacggcgag ggcggatacg gcggcggtcc gtacggtggt
     2641 cagctcgatc ccgtccagca gggccagcgg ggagagcgtc ggggcgtcga agagcaggta
     2701 acggccctgg acgcgggcga gtccggcggc cggattggcc ggggcgacgg tggcgatctt
     2761 gacgcctgcg gaccggcgcc ggtgcgcggg catgaggagc aactggccgt gctcgaccgg
     2821 tacgacggtg cggggcggat cggactccgg atccgttccg tcccgcaggg cggcgcgcag
     2881 tgcgccgatc gccgcggcca tcggcagggc acgccggagt gcggccccgt cgatggtccg
     2941 gggcgcggtc acaggacgaa tccggcgggg aaggggtcct ccgggtcgcg gtggaactgg
     3001 gcggtgccgg tgatccacgc acggcccgtg atggtggggg tcacggtggt gaggtcgccg
     3061 agccgcccct cttcgaccag gcggccggtg aagtgcgtgc cgatgaacga ctcgttgtcg
     3121 aagtcggtgt cgagggtgag ttcgccgcgc gcgtgcagct gggccatccg ggcgctggtg
     3181 ccggtaccgc acggcgagcg gtcgaaccag ccggggtgga tgaccatggc atgccgggag
     3241 cggcgggcat cggacccggg agcggccaga tacacatggt ggcagccgtt gatctcggga
     3301 ttgccggggt ggacggggcg gtcctgttcg tcgacggcgg ccatgacggc cagaccggcg
     3361 tcgagaatgc gctgcttctc ggcgcgctcg aacgggatgc ccagggcctc cagcgggacg
     3421 atggcgtaga agttgccgcc gaaggcgagg tcatagggga tgtcgcccca gggctcgacc
     3481 cgtaccgtcc ggtccagtcc gagcgcgaac gacggcacat tgcgcagggt caccgacttc
     3541 gcggccccgt ccaccacccg gacctcggcg acgacgagtc cggcgggggt gtcgagacgg
     3601 atggtggtgg tgggttcggt gacctcgacc atgccggttt cgacgaggac ggtggcgacg
     3661 ccgatggtgc cgtggccgca catcggcaga cagccggaca cctcgatgta aaggaccccg
     3721 aagtcggcgt cggggcgggt cggcggctga aggatggcgc cgctcatcgc cgcatgcccg
     3781 cggggctcgt tcatcaggaa cgtacggatg tggtcgagat ggtcgatgaa gtaccggcgc
     3841 cgggcgaaca tggtgtcgcc ggggatgacc ccgacgcccc cggtgatcac ccgggtgggc
     3901 atcccctcgg tgtgcgaatc gaccgcgtgg aagattcggt tgctgcgcac gtgtctaccg
     3961 cagtccttcc gcgagggcct ttccggtggc ctgccgcacg ctctcctcgt gctccggggc
     4021 cagcggcagg cgcggctgcc ggcagggacc gccgtagcgg ccggcgagat ccatgctgag
     4081 cttgatcgcc tgcacgaact cgatccgcga gtcccagcgc agcagcgggt gcagcgtccg
     4141 gtagagcggc agcgcggtgg acaggtcacc ggcggacgcc gcctgccaca gttcgacgca
     4201 ggcgcgcggg aacgcgttgg tgaagcccga cacccagccc ggggatccgg cggccgccag
     4261 ctccagcgcg acatcgtcgg cgccgcacag cacatcgagt ccgggggcca gttcggcgat
     4321 ccggtaggcc cggcgcacat cgccggagaa ctccttgacg gcgacgatca gcccttcgcc
     4381 gtacagctcg gccagcagct ccggcaccag atcgaccttg gtgtcgaagg ggttgttgta
     4441 cgcgacgacc ggcagtccgg ccctggcgac ctcacggtag tgcgcgacca cggcacgccg
     4501 gtccgcccgg taggcgttcg gcggcagcag catcaccgca cgggccccgg ccgcaccggc
     4561 gtgttcggcc caccgccgcg cttcgtcggc gccgtacgcg gccaccccgg gcatcacggt
     4621 gaagccgggg gtgtccgcgg ccacctcgac cgcggtggtc acgacacggg cgcgctcctc
     4681 gggggtgagg gtctggtact cgccgagcga accgttcggg gtgacgccgt gacagccgtt
     4741 ctccgcgagc catccgagat gttcggcata gccgtcgaag tccgccgtca gttcgccggt
     4801 ccgcgactcc cgccacggga gcgcggtcgc caccagtact ccgtgccagg gctgtgcttg
     4861 cgcgttcatg tgacctccac ggtttgcgta tccgggacca ctgagtctgt gcaatgttac
     4921 attgcattgg ctctcggggt ggggtgaatc atggggtgtg aggtgcaggg gtggggtgga
     4981 tggagcttct caatcgcgcc tcagcgggcg ggcattgacg actcatcagt tccggcttca
     5041 ggaacgatgc cggagccgga ttcggggtcc gcgccggggt cgccggcggc gaggatgccg
     5101 agcggcagcg gctgcgcgat gggccggccg gccatccccc gcagttcctc cgcggtcgcc
     5161 tcacgtccgg atcgtgcggc gaccaggcag gcggtggcgt aaccgcagac ccgtccctgg
     5221 cacagcccca taccggggcg cgcgtacagc ttcaccgcgc gggggtcggt cgcgcccagt
     5281 tccgtgacgg cctcggtggc ggtccgcacg gtgacctcct cgcaccggca caccacggtt
     5341 tcggggcgca gccaccccag ccagccgggg cggaccggaa acgcccggtg cagcgcggcg
     5401 gcgaactgcc gctgtgcggt acgccgccgc accagacggc ggaccaccac cgggtcggcg
     5461 acggcggcgc cgacggcctg ggcggcgtgc agcccggcga gttcgccctc ggtcagggac
     5521 agggcggcgc cgccgacgcc gcagacctcc ccggcgaggt agacgccggg gaccgaaccg
     5581 cgctgccggg cgtcggcggt ggcgaccagg ctgccgtcga tatcgcgacg ggtcgcgcag
     5641 ccgagctgga gcgggatctc gatctgcggg gtgaagccgt agccgacggc gagggtgtcg
     5701 cagtcgagcc ggcgctcgct gcccggtacg acccgccacc gcgcgtcgac gcgggccacc
     5761 gtgaccccgg tgacctcgtc ctcgccgtgc gcggcgacca cggcggtgcg ggtccggtac
     5821 ggcacgcgat ggcgcagcag cgtccgcagg aaccccgcgc cctcggcgag tttggcggga
     5881 tttgcggcca cggtgagcgg ctgccgcacg aacgaggcgg gcagtccggc ctcggagacc
     5941 ccgaccacgc gcgcgcccgc ctcggcgagt ccggcggcga cggccgggag gaaggggccg
     6001 gtcccggcga ccgcgatccg ccgtccggcg accacaccgt gacccttcag cagcgcctgc
     6061 gcaccgcccg cggtgaacac cccgggcaac gtccagccgg ggaaggggag ttggtggtcg
     6121 taggcgtcgg tggccacgac gaccgcggtg ctctccacga cctgctcggt cccgtcgtgg
     6181 agggtgtgtg tacggaatcc gccggccgtc cgcccggtat gccagacggc gtggccgggg
     6241 aggtggtgga gccggccggc gtgggcggcc agtccgtggc gcagtcgctg gaaggcggcc
     6301 cagccgtggt gcgccgcgcc cgtgtcgccg gggcggtggc gccagtactg cccgccgacc
     6361 cgcgggcccg cgtcgaccag cgcgacccgg gcgcccgcgg cggccgccgt gaccgccgcc
     6421 gcgagaccgg cggggcccgc gccgagcacc gcgagatcgt acggcccgtc agcacgcgag
     6481 gtcgtcacgg ccggctcctt cctgggtcct gaccaggtct cccggcgcgg cctcgaccag
     6541 gcaggcccgc tggttcgggc gcccgttgac ggtgaccagg cagtcgaagc agaccccgat
     6601 gccgcagaac atcccgcgcc cgtcgcccgc gacccgggta cggcgccagg agcggtgtcc
     6661 ggcggcgagg agcgcggcgg cgatgctctg gcccggctcg gcctcgatcg tcagctcgtc
     6721 gaagcggaag gggaagggga aggggaagga cgggcggcgg ggggcggccg gcccgcccgc
     6781 cccggcacgc gtatcggtgc tcatacggtg gtctcctcga accgttccgg gcggaagggg
     6841 gtgagatcca gatcgggcgc cgcccccgtg atgacctgcg cgatcagatg acccgttgcg
     6901 gcggacagcc cgatcccggc gccctcgtgc ccgcaggcgt gcagcagccc gggggcgcgc
     6961 ggatcggcgc cgatcaccgg cagatggtcc gggcagtacg gccggaatcc caggtagctg
     7021 cgcagcagtg cgacctcggc gaggaacggg aagaccccga tggcctgcgc ggcgagtctg
     7081 cgcagcaccg gcagggatac cgaacggtcg aagccgaccc gctcccggct ggccccgatc
     7141 aggaccgtgc cggcccgggt gccctcgacc acgacggagg tctggagccc cgcgtcgccg
     7201 ctggcgacat tggcgacgta ctccgcggtg tacaccttgt gccgcaccac ccggggcagc
     7261 ggctcggtga ccaggatgaa ccccttgcgg ggcaggacgg gcagcggcac gcccgccagc
     7321 ccggccagct cgccgcccca ggtgccggcg gcgttgacga cccggccggc ggggatcagc
     7381 ggtgccgtgg ccgagtcggt acggacgccg gtgacggcgc cacgactgtc gcgtacgaac
     7441 ccggtgaccg cggtgcccgg caggaccgtg gccccgtgct cccgggcgtg ccgcagcagc
     7501 cgggccgtcg ccagcaccgg ctggacctgg cagtcctgcg ggtagtacgc gccggcggcc
     7561 acctcgggcg tgatgttcgg ctccagtccg gtgacctcgt cgcgggagac gtcgaccgcg
     7621 tcgataccgg ccgcgcgctg gacggcggtc agcttccgga gcccgtcggc cgtcgccggc
     7681 gaggcggcca ccatcacgcc gcccttctcc tggtactcca gccgctccgc gcccagcgcc
     7741 tgcgcggtcc gcgcccacag tcgcgaggac agcagcgcca gttcgagttc ggctccgggc
     7801 tccttgtcgg agagcaggat gttgccctcg cacgccgcgg tggtggcgcc cccgatcgcg
     7861 ccccgttcga ccacggtgac ccgcagcccc gccgaggtgc agtggtaggc gcaggcagcg
     7921 ccgaccatgc ccgctccgat gacgacgacg tcaggagacc gattcgtcga gcgatccatc
     7981 gcagtagaat gtcacgttgc attgccccgc gccagggccc gtacggcaac cggcctacgg
     8041 gcatctgccg taccgccggg ccgagttggg gcggagacgc cggcagggcc gggaggtgct
     8101 gccgggaatc gcggcggtgg ccccggcggg attgtcagtg cggtcgggct ccgcgaacat
     8161 gcctccatga gcctgatgac cgatggcaaa gctcctggtc cggtgcgctt ctatctcgcc
     8221 tgtgaccgtg gcaagtgcac cgagagaatc agctttgacc tcgtgatctc cgagccgcgg
     8281 ccggacgaag aggcggactt tctcggccac atacttcacg aggcccacca cgcggccccg
     8341 ctcatcgcgg acctgggctg ggtcttcatc cagggcgggg aaggttactg gtgccccgcg
     8401 tgcgccggcc ccacccgcga caccggcacc cccgacccct cgtagacgcc cacaccagga
     8461 cggcgccggt cctcatcgga ccggcgccgc ccgctgtcgg cgcggagcgg tggcccggaa
     8521 cggtggcccc gggggagtgg tccccgagcg ccggttcccc tcgatccgcc gaaaagcgga
     8581 tgacgggcgc ggaacagttg tcgcggccgc tcggaaagtt ccggcgggaa agtgagttcg
     8641 ccgaatacag gcaccaatgg cacaggaaaa aatccgtatt tctccctcaa tcgggtggac
     8701 cgtcggaatc gcccttcaag agacctgccc gcgcccctaa agtcatgcct cagcaccgca
     8761 tcacagcgga attcctctcg cgtatctgaa gccgagaggc gcggtccgcg ggcgcccggg
     8821 gcgccctgcc gggcgtggcc cagaattccc gttccgagga aggcacaggg cattggcagg
     8881 ttcttcgctg gaagcaatcg tgctggtcga catcgccgtc gtgctgctga taggagtcgc
     8941 tctcgcaccg ctgcggaccc gtctgcgaca gccggtggtg gtcggggaga tcgcggtcgg
     9001 tgtgctgctc gggccgagtg tgctgggcct gctgccgggg aatctgccgg cgaaggtctt
     9061 cccggtcgag gtgcgcagcc acctgtcggt gatcgcccaa gtaggcatcg tcctcttcat
     9121 gttcgtggcg ggctgggaac tcgaattcca tttgctgcgt ggccgggtcc gctccgtact
     9181 cgccattacc gccgcgtccc tgaccgtgcc ctgcgcactg gcctccgcag tggctttcgc
     9241 gctcttcgcg ggacattcga gtctggcgcc ggcccatatc tcgccgccgc tcttcatgct
     9301 cttcatgggc gtcgtcctgt cggtcagttc cctgtccgtg atgatccgca tcctgggcga
     9361 gaacgggctc cagggcacca ccgccggcgc catggccacc gcgtccggcg cgatcaccga
     9421 cctcgtggcg tggttcgcct tcgtctgcct gatcacggcg gcgcagggac tgagcaccgg
     9481 gaatctgctc ctcaccctgg gccgggtcac ggcctactcg ctggccatgg gcttcgtcgt
     9541 ccgcccggtc ctgcgcgcgc tgctgcgccg ggtccacccg cagggcggac actcgctgtt
     9601 gctgatcttc gccgtcggca gcggtgtctt cgtgtccgcg tggttcgcca cctgcatcgg
     9661 gatccatgcc gtgatcgggg ccttcacgtt cggcctggtc atgccacgcg atctggaccc
     9721 cgccgtgcgc cgggcgatcg cgacgcccct gcaccatgtg ggagcgctac tgatccccgt
     9781 cttcttcgga ctgaccggcc tgtccgtcga catgggcgcg ctcggctggg gcggagcgct
     9841 ggcgctcacc gcgttcttcg tcgtctcgct gggcggcaaa ttcaccggaa ccgcggtcac
     9901 cgcgcgcctg ttccacctgc cgtggcgcga agcggcgacc gtgggcgtgc tggtcaacag
     9961 caaagggctt ggcgaagtcc tgatgctcac catgggccgg gaagcgggcc tcatcaacga
    10021 ccagctcttc tccgcgctgt tcctgaccgc gctgctcatg accgcctccg tcaacccgct
    10081 ggtacgacgc ctgacgccga aggccggcag tacggacccc ggcgccgccg tccccgcaca
    10141 gcagcaggga ccgcacgcgg ccccggagtt gagcgacgac cacaccaccc ggggcccgac
    10201 ctgagaccgg gacagggccg gccgtgcggg gccggccctg tcccgggcgg tcgcgagcgc
    10261 cgcggcctgc cgcgcggcta caggcgccgg ttggccagca ccgcgacggc gcggcggaca
    10321 cggtcggtct cggcaagatc cacgtcggcg gtgagcagat ccggtgccgc gcccagccgg
    10381 gcgcgtacgg tgccgtcggg ggcggccagg aggctgtggc cgataccggt cggggccttg
    10441 gtgggcagtt ccggatcggc cagcggatcc ggggcggcgt gtccgacggc cgccagccag
    10501 accgtcgcgt ccagggcgcg ggcccgcacc agcagttccc actgcgccag cttgccgggg
    10561 ccggcgcccc aggaggcggg caggacgctg acggtcgccc cggcgtccgc gtgtgcccgg
    10621 aacaactcgg ggaaacgcag gtcgtagcag gtggccaggc cgatacggac cccgtcgaca
    10681 tcgatggtca ccacgcgggt gcccggggcg acgctgtcgg actcccggaa cccgaacgcg
    10741 tcatacaagt gaatcttgtc gtaggagtcc tccacgccgg gcccggtggc cagcagggtg
    10801 ttcacgaccc gtccgtccgg cgcgggcgtg aacataccgg cgaccaccgt caccccggtc
    10861 tcccgggcca ccgcccgcac gccctcggcc cacggtccgt ccagcggttc cgccaccgga
    10921 ccgagcggca ccccgaagcg caccatggcc gcctcgggca gcaccgcgag ccgcgccccc
    10981 gcgtgcgccg cgcggcggac gtggtcctgt acggccgcga ggttctcctc cgggtcggtc
    11041 gatgccgtca tctggcagag tgcgatgcgc atgcggctcc ttctctcatg gtgatcgatg
    11101 atcccctcgt gatgaccgat gattgacgat cgctgatcca ttgtggtcgg tggtggccgg
    11161 tggcgaccgg tgtccgtggt agcacgaccg ggactcggcc cccgtacgcg gtcgtggccg
    11221 ggcatcgtcg cccgcagccc gacggcactc cggcgtccac gtcgcgatca gcctcgcgcc
    11281 ccggccgcac acccgcaatg gtgaccacgg cggtggcggc cgaaacggac gcacatagac
    11341 gcgaacccgg cggcgccggg cagccgttgc cgtccggggc gagcccgccg cgcgcgggcg
    11401 ctcagccggg ccaggtccag tcggcgacct cgggcaggtc ggtgccgtgc gcccggatcc
    11461 aggcgtggtg gcgggtgcgg gcatcggcca tcgcctggcg cagcgcgacg gcgcgtaccg
    11521 cgaggccggg gacccggtcc acgacgtcca tcaccagccg gtagcggtcc agatcgttgc
    11581 ggacaaccat gtcgaagggc gtcgtggtgg tgccctcctc cttgtacccg cggacgtgca
    11641 gatgctgatg gcccgcgcgg cggtaggcga gccggtggat cagccagggg tagccgtgat
    11701 aggcgaagat gaccggtctg tcgcgggtga acacggcgtc gtactcgaag tccggcatcc
    11761 cgtgcgggtg cgcgtcgcgc ggcagcagcc gggtcatgtc gacgacattg accacccgca
    11821 ccgccagctc cggcagatgg tgccgcagca gaccggcggc ggcgagcgtc tccagggtgg
    11881 gtacgtcgcc ggcgcaggcc aggacgacat ccggttcgcg gctgccgtcc tcggtgcccg
    11941 cccacgccca gctgccgacg ccgcgcgcgc agtgggcgcg ggcctcgtcg agcgtcagcc
    12001 agtcgaagcc cggctgcttg ccggccacga tcacattgac gtagtcccgg ctgcgcagcg
    12061 cgtgatcggc caccgacagc agggtgttgg cgtccggcgg cagatagacg cgcacgacct
    12121 cggggctctt gtccaggatg tgatcgacga aaccggggtc ctggtgggag aagccgttgt
    12181 ggtcctgccg ccagacatgc gaggtgagca ggtagttgag ggaggcgatc gggcgccgcc
    12241 agggcagccg gcgggcggtg cgcagccatt tgatgtgctg gttgaccatg gagtcgacga
    12301 tgtgcgcgaa cgcctcgtag ctggcgaaga gcccgtggcg cccggtgagg agatagccct
    12361 ccagccaccc ctggcacagg tgctcggaga gcacctccat gacccggccg tcccgggcga
    12421 ggtgttcgtc ggtgtcgagg gtctcggcct gccacgcctt accggtggcc gtgtagaggg
    12481 cgtccaggcg gttcgaggcg gtctcgtcgg ggccgacgac gcggaagtcc cggcgctcgg
    12541 cggtggccgc catgacggcg gccagcagac cgccgagcac ccgggtgggc tcgtgcaggg
    12601 ccgtgcccgg ccgctcgacc ttcaccgcgt accgatccag cggcggcacc ggcagctccc
    12661 gcagcagcag accgccgttc gcgtgcggcg aggcacccag ccgccgcgcc ccctcgggta
    12721 tgtacgcgag gacctcgggc cgcggccgcc ccgccgcgtc gaacaactcg tccggccggt
    12781 acgagcgcag ccacgcctcc agctgccgca gatggtcggg gttctcgcgg acctcggaca
    12841 gcgggacctg gtgggcgcgc caggtcccct cgacgggcac cccgtcgacc tcgtgcgggc
    12901 cggtccagcc cttgggtgtg cgcagcacga tcgcgggcca gcgcggacgg tcgcgcacct
    12961 cctcggaacg ggcccggcg
//
