LOCUS       NZ_NDXL01000001        10915 bp    DNA     linear   CON 27-APR-2024
DEFINITION  Streptomyces kasugaensis strain BCRC 12349 scaffold1, whole genome
            shotgun sequence.
ACCESSION   NZ_NDXL01000001
VERSION     NZ_NDXL01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces kasugaensis
  ORGANISM  Streptomyces kasugaensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Comparative genome analysis for kasugamycin overproduction
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Comparative genome analysis for kasugamycin overproduction
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030,
            China
REFERENCE   4  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. May-2015
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 188.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002261115.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 02:42:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,951
            CDSs (total)                      :: 7,863
            Genes (coding)                    :: 7,657
            CDSs (with protein)               :: 7,657
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 4, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 4, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 87 of 206
            Pseudo Genes (incomplete)         :: 170 of 206
            Pseudo Genes (internal stop)      :: 23 of 206
            Pseudo Genes (multiple problems)  :: 65 of 206
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:37:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4034093
            Orig. end                         :: 4045008
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NDXL01000001.1.
            Bacteria and source DNA available from BCRC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10915
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="25"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10915
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10915
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5915](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5915)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            11..454
                     /locus_tag="B7024_RS17420"
     CDS             11..454
                     /NRPS_PKS="Domain: Polyketide_cyc2 (3-146). E-value:
                     3.6e-24. Score: 77.7. Matches aSDomain:
                     nrpspksdomains_B7024_RS17420_Polyketide_cyc2.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038765.1"
                     /locus_tag="B7024_RS17420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SRPBCC family protein"
                     /protein_id="WP_052858217.1"
                     /transl_table=11
                     /translation="MGQVEATTQREIAADPEDVFDALADYSGTRRQLLPEHFSEYEVRE
                     GGDGKGTVVHWKLQATSKRIRDCLLEVDEPTDGQLVEKDRNSSMVTTWVVTPAGEQRAQ
                     VVVTTTWNGAGGIGGFFEKTFAPKGLGRIYDAILANLAAHMEK"
     aSDomain        20..448
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B7024_RS17420_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="3.60E-24"
                     /label="B7024_RS17420_Polyketide_cyc2.1"
                     /locus_tag="B7024_RS17420"
                     /protein_end="146"
                     /protein_start="3"
                     /score="77.7"
                     /tool="antismash"
                     /translation="VEATTQREIAADPEDVFDALADYSGTRRQLLPEHFSEYEVREGGD
                     GKGTVVHWKLQATSKRIRDCLLEVDEPTDGQLVEKDRNSSMVTTWVVTPAGEQRAQVVV
                     TTTWNGAGGIGGFFEKTFAPKGLGRIYDAILANLAAHME"
     misc_feature    545..559
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     gene            638..3154
                     /locus_tag="B7024_RS17425"
     CDS             638..3154
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560990.1"
                     /locus_tag="B7024_RS17425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_172450754.1"
                     /transl_table=11
                     /translation="MERGPSHGDDTAGAAPTVPVPREAVPGRLPLAVVVVDAAGRISHW
                     SSGARRLFGPSRQEAVGAAAVDLMPVTGALGDREWDEPPDGADAAAADDDVPDAGPGAL
                     PGGGAAYPTAGRARMAGAGSAPVDLLWWAYPLIGPGTERTLVLAADAAQLHGPGDDGSG
                     RPGAGSERFTPAFARHTDLPDAAELARRLPEILPSMNPLDSARIVSQVLELGYPVLEIS
                     RHERFPVTPDWGVPRRVERRTRLLRAASAVADDPRAAAAAAAELASDLEYAAVRERLEF
                     LNEVSGRIGTSLDLARTIQEVSAAVVPRFTDVAGTYLREQVVAGEGFPDGPPDATTLWH
                     RVAVEHNDEPGRWDDVVPVGESMPFPVQTPFFQCMTTGQPVLVPRISDDMGNAIAAQFE
                     KRDISPLINRRSLLVVPLKARNVVLGFMILLRHPERTEFNDMDRVTGAELAARAGLVLD
                     NARMYTFQENVAETLQDSMLPHIAPRMAGCDTATRYLPGTRLGRVGGDWFDTIKLPGSR
                     TALVVGDVMGHGLNSAAMMGQLRTAVQTMAAIDMHPAQLLRSLDDLAQRLGDSYLATCL
                     YAVYDPIGQRLTIANAGHIPPVLVRAVDGRSELLELPTGAPIGVGGVPFEAVTVPVAAG
                     DRLVLCTDGLVEVRGEDIGVGLAALAESAAHPAASMDDACDAIIRALNVQDDRKDDVAL
                     LMARLNGIAPDAVAEWRLAIDPREVGRARALVGRRLADWGLDSSAEPAALMVSEVVTNA
                     VRHAHTRQVVLRLVRTDALLCEVSDDDHALPQLLNAGADDEFGRGLRVVSRLAREWGTS
                     RTDTGKTVWFELSLPNTRRTRGPHAG"
     misc_feature    1181..1183
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3208..3999
                     /locus_tag="B7024_RS17430"
     CDS             3208..3999
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981795.1"
                     /locus_tag="B7024_RS17430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_094792503.1"
                     /transl_table=11
                     /translation="MSAASPFTSPSAQAWEGFWRAAPTGAGEVFWDADPALAVQLHLPL
                     FAGHFDPGLPVVDLGCGNGTQTRHLAGRYARVVGIDLAGAAIGRARAEDVHGTADFRCL
                     DATDPDAMRALHAELGDAHLYLRGVLHQCAPGDRARIVANIAVLLGTRGRVFAVEPAAS
                     AKDVLRSLAQSPTGPPAKLRAVLSHGISPGDMADTAVPGLLAAQGLRVVASGRLPLATT
                     EYRPDGSRIEIPTNWLVAAAAGGSAGPVRRPGPGAGRLPSR"
     misc_feature    3216..3231
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.73"
                     /tool="antismash"
     gene            complement(4067..4759)
                     /locus_tag="B7024_RS17435"
     CDS             complement(4067..4759)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151397.1"
                     /locus_tag="B7024_RS17435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HAD family phosphatase"
                     /protein_id="WP_052858223.1"
                     /sec_met_domain="HAD_2 (E-value: 2.9e-30, bitscore: 103.7,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSHRILSWTPAAVVFDCDGTLMDTERHWQDAREITLEEFGFTPD
                     PGFAERAKGLHYTECGHLMAREAGHPELGDELTARLLRHFRTLAADHPVTMPGARELVV
                     AVSKFAPLAIASNCPREVVEASLATAGLLSHFDHIVVPDDVIRPKPHPDVYLTAARQCG
                     TAPADTLAVEDSHCGIQSAARAGLRVIGVGPWPGEQVTALVDLWVRSLDEPRVSGWAGN
                     RIPRQVSR"
     misc_feature    4918..4938
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.82"
                     /tool="antismash"
     misc_feature    complement(4919..4939)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.53"
                     /tool="antismash"
     gene            complement(5001..5915)
                     /locus_tag="B7024_RS17440"
     CDS             complement(5001..5915)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543192.1"
                     /locus_tag="B7024_RS17440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_094792504.1"
                     /sec_met_domain="AfsA (E-value: 1.8e-19, bitscore: 67.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTSVGETAGELRPLSWSRTVAREMVHRTSLAEVLLTDVRRTGDG
                     SFEAAASWPRSHPTFPRDGADLHSPLIVVETLRQLGIYLPLRYFGVPATAHLLITDLFF
                     ALTPAAEPRAASAATDVTCLTGVSEIRTGADGAVTGLRLEVTFLAGGTAFARAGGGARF
                     LDARRYTVLRGAVTTVRRTSRTAVRVRPEATGPAVADPRDIVIGLDGGTLLVEPVDPKH
                     PFFFDHATDHVPGMVLLEAARQAAALESHGTLLRPTAGRLRAVRFTEFSPPARVLCVPH
                     HGTCVFRVFQGGEQKAFGTLQYR"
     gene            6027..6740
                     /locus_tag="B7024_RS17445"
     CDS             6027..6740
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543193.1"
                     /locus_tag="B7024_RS17445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_094793242.1"
                     /transl_table=11
                     /translation="MQVRARTTRRSLLETAARLFDERGYAGTSISDISTRSGRTSGAIY
                     FHYASKENLALAIVEEHFATWPELIARHRAARLPALDTLVALSFDVARAFRDDVVIRAG
                     SRLWMERKAIDAALPTPFVGWTATVTELLEEARAEGTLAAAIDPGTAAHGVVCAFFGLH
                     TVADALDGRSCIEERLHGLWLLLLAALQADPAPAALLSRVHDRAGAAEQRRPARPAATG
                     PARSGLAAVGAARAK"
     misc_feature    6945..6957
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.37"
                     /tool="antismash"
     gene            7202..8458
                     /locus_tag="B7024_RS41890"
     CDS             7202..8458
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075: alkaline serine protease, subtilase family"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /locus_tag="B7024_RS41890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="S8 family serine peptidase"
                     /protein_id="WP_094792505.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 2.3e-16, bitscore:
                     57.9, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDVRNRQLPLQRGVTVAIGALGCTVLLAGGTAGARPVAGAPVARV
                     AAAGGGTVLPQIPDGPVAEGKCTKESARTISQTPWAQKALGLSEVRPLSQGGGVTVGVV
                     DTGLDAGVAALKGRASGGGGKDCAGHGTFVAGIVAGAAQDGVGFAGVAPRARVLGETVS
                     GADAGALAGGIEDAVAGGAKVVLVAASATESDARLQTAVDKAAAKDVLVIAGATAGSKG
                     AQAYPGGLSGVLSVAAVGVDGKPYPGRTGGQSTGASLPRVDLAAPGDRVVSVGPGGGHF
                     TGGGDSVAAAFVAGTAALVRAHDPGLSAAQVADRLRATAARGPGAVPDARFGYGLVDPP
                     GAVGAVSVAASGTGARTRTESAPALDGTYRVPEQPPATSPAVAWAVVGVAALVSLLVGG
                     AALVIPRGRARGWRPGRLG"
ORIGIN
        1 gaagacgcac atgggtcagg tcgaggccac cacacagcgc gagatcgcgg cggacccgga
       61 ggacgtgttc gacgcgctcg ccgactacag cggcacgcgc cggcagctgc tgcccgagca
      121 cttcagcgag tacgaggtca gggagggcgg cgacggcaag ggcaccgtcg tccactggaa
      181 gctccaggcc accagcaagc ggatccgcga ctgtctgctg gaggtggacg agccgaccga
      241 cggccagctc gtggagaagg accgcaactc ctccatggtg accacctggg tcgtcacccc
      301 ggcgggtgag cagcgcgccc aggtcgtcgt caccaccacc tggaacggcg cgggcggcat
      361 cggcggcttc ttcgagaaga ccttcgcccc caagggcctc ggccgcatct acgacgcgat
      421 tctcgccaac ctcgccgccc acatggagaa gtgagccccg cgccggcgca ccggccggcc
      481 cgccccgggc gtggccaccg ccgcatccgg ggcgcgtggg cggccgggcc aatgcgcccg
      541 cgcacgacct cttcggccaa tgccgcccac gccaccgctc catggctcta cggtgggacc
      601 ggtggtgccg gaccggtgca cgaaggagca accgcccatg gagcgtggcc cgtcgcacgg
      661 tgacgacacg gccggcgcgg cacccacggt gcccgtgccc agggaggccg tgcccggacg
      721 gctgccgctc gccgtcgtgg tggtggacgc ggccggccgg atctcccact ggagcagcgg
      781 cgcgcgcagg ctcttcgggc cctcccgcca ggaagcggtg ggtgcggccg ccgtcgatct
      841 gatgccggtc accggcgccc tcggcgaccg ggagtgggac gagccccccg atggcgctga
      901 cgctgccgct gctgacgatg acgtgccaga tgcaggcccg ggggctttgc ccggcggcgg
      961 cgcggcctac ccgaccgcgg gccgggcccg gatggccggc gccgggtctg ccccggtgga
     1021 cctgctgtgg tgggcgtacc ccctgatcgg accgggcacg gaacggacgc tcgtactcgc
     1081 cgcggacgcc gcgcaactgc acggaccggg cgacgacggg agcggccggc ccggcgcggg
     1141 gagcgagcgg ttcaccccgg ccttcgcccg gcacaccgac ttacccgacg ccgcggagct
     1201 ggcccggcgg ctgccggaga tcctgccgag catgaacccc ctggacagcg cccggatcgt
     1261 ctcccaggtg ctggaactgg gctatccggt actggagatc agccggcacg agcggttccc
     1321 ggtgaccccg gactggggtg tgccgcgccg tgtcgagcgg cgtacgcggc tgctgcgcgc
     1381 ggcgagcgcg gtggccgacg atccgcgggc cgccgccgcg gccgccgccg agctggcctc
     1441 ggacctcgaa tacgccgccg tccgcgagcg gctggagttc ctcaacgagg tcagcgggcg
     1501 gatcggcacc tcgctcgatc tggcccgcac gattcaggaa gtcagtgccg ccgtcgtacc
     1561 gcgcttcacc gacgtcgccg gcacctatct gcgcgaacag gtcgtcgccg gcgagggatt
     1621 ccccgacggg ccgccggacg ccacgaccct gtggcaccgg gtcgccgtcg agcacaacga
     1681 cgagccgggg cgctgggacg acgtcgtccc ggtcggcgag tcgatgccct tccccgtcca
     1741 gacaccgttc ttccagtgca tgaccaccgg acagccggtc ctggtgccgc ggatcagcga
     1801 cgacatgggc aacgccatcg ccgcgcagtt cgagaagcgg gacatcagcc cgctgatcaa
     1861 ccggcggtcg ctgctggtcg tcccgctcaa ggcgcgcaac gtggtgctcg gcttcatgat
     1921 cctgctgcgc catccggagc gtacggaatt caacgacatg gaccgggtga ccggcgccga
     1981 actcgccgcc cgcgccgggc tcgtgctgga caacgcccgg atgtacacct tccaggagaa
     2041 cgtcgccgag acgctccagg acagcatgct gccgcatatc gcaccgcgga tggccggctg
     2101 cgacaccgcg acccgctatc tgccgggcac ccgcctgggc cgggtcggcg gcgactggtt
     2161 cgacaccatc aagctgcccg gctcgcgcac cgcgctggtc gtcggcgacg tcatgggcca
     2221 cggcctcaac tcggccgcga tgatgggcca gctgcgcacc gccgtccaga ccatggcggc
     2281 catcgacatg caccccgcac aactgctgcg cagtctggac gacctcgccc agcggctcgg
     2341 cgacagctac ctcgcgacct gtctgtacgc ggtctacgac ccgatcggac agcggctgac
     2401 gatcgccaac gccgggcaca tcccgccggt gctggtccgc gccgtggacg gccgcagcga
     2461 gctcctggaa ctccccacgg gcgcgccgat cggcgtcggc ggggtgccgt tcgaggcggt
     2521 gacggtgccg gtcgcggccg gcgaccgcct ggtgctgtgc acggacggcc tggtggaggt
     2581 gcgcggcgag gacatcggcg tgggcctcgc ggcgctcgcc gaatccgccg cgcaccccgc
     2641 cgcctccatg gacgacgcct gcgacgccat catccgggcg ctgaacgtcc aggacgaccg
     2701 gaaggacgat gtggcgctgc tgatggcgcg gctcaacggc atcgcgccgg acgccgtcgc
     2761 cgaatggcgc ctggcgatcg acccccggga ggtcggccgg gccagagcgc tggtgggccg
     2821 ccggctcgcc gactgggggc tggactcctc ggccgagccc gccgcgctga tggtcagcga
     2881 ggtcgtcacc aacgccgtac ggcacgccca cacccggcag gtggtgctgc gcctggtccg
     2941 tacggacgcc ctgctgtgcg aggtctcgga cgacgaccac gccctgccgc aactgctgaa
     3001 cgcgggcgcc gacgacgagt tcgggcgcgg gctgcgggtg gtgagccggc tggcacggga
     3061 gtggggtacg agccggacgg acaccggcaa gaccgtctgg ttcgagctgt ccctgccgaa
     3121 cacccggcgt acccggggcc cgcacgccgg gtgacggatc cggcgcccct cttgcgggcc
     3181 gcgcaccggc gggcgtagca aggggccatg agcgctgcga gtcccttcac gagcccctcc
     3241 gcgcaggcgt gggagggctt ctggcgcgcc gcgccgaccg gtgcgggcga ggtcttctgg
     3301 gacgccgacc ccgccctggc cgtgcagctc catctgccgc tcttcgcagg gcacttcgac
     3361 cccgggctgc cggtggtcga cctggggtgc ggcaacggca cccagacgcg ccatctcgcc
     3421 ggccgctacg cgcgcgtcgt cggcatcgac ctcgccggcg cggcgatcgg ccgcgcacgg
     3481 gccgaggacg tccacgggac ggccgatttc cgctgtctgg acgccaccga cccggacgcg
     3541 atgcgtgccc tgcacgctga actgggcgat gctcacctct atctgcgggg cgtcctgcac
     3601 cagtgcgcgc ccggcgaccg ggcgcgcatc gtggcgaaca tcgccgtact gctgggcacc
     3661 cgagggcggg tcttcgcggt cgaaccggcc gcctcggcca aggacgttct gcggtccctc
     3721 gcgcagagcc ccaccggacc gcccgcgaag ctgcgcgcgg tcctttcaca cggcatctca
     3781 cccggtgaca tggccgatac ggccgtcccg ggactgctcg ccgcccaggg gctgcgcgtc
     3841 gtcgcgagcg gccggctgcc gctggcgacc accgagtacc gccccgacgg atcgcgcatc
     3901 gagatcccca cgaactggct ggtggcagcg gcggccggcg gatcggccgg gccggtccgc
     3961 cgccccggtc ccggggccgg ccgcctcccc tccaggtagc cggtcgcggg gtgcccgcgg
     4021 tggcccccgg cgccgccggc gctccggcgg cgcccggggc ggcgggtcag cgggacacct
     4081 ggcgggggat gcggttgccc gcccagccgc tgacccgcgg ttcgtccagt gaacgtaccc
     4141 acaggtcgac cagggcggtg acctgttcgc ccggccaggg gccgactccg atgacccgca
     4201 ggccggcgcg ggccgcggac tggataccgc agtgcgaatc ctcgacggcg agggtgtcgg
     4261 cgggcgcggt gccgcactgc cgggcggcgg tgaggtagac gtccggatgg ggtttggggc
     4321 ggatgacgtc gtcgggtacc acgatgtggt cgaagtggct gaggaggccg gcggtggcga
     4381 ggctggcctc gacgacctct ctggggcagt tgctggcgat ggcgagcggg gcgaacttgc
     4441 tcaccgccac gacgagttcc cgggcgccgg gcatggtgac ggggtggtcg gcggccagcg
     4501 tgcggaaatg tctgaggagc cgggcggtga gctcgtcgcc gagttcgggg tgtccggctt
     4561 cacgggccat caggtgtccg cattcggtgt agtgcaggcc cttggcgcgt tccgcgaatc
     4621 cggggtcggg ggtgaacccg aactcttcca gggtgatttc ccgtgcgtcc tgccaatggc
     4681 gctcggtgtc catcagggtg ccgtcgcagt cgaagacgac ggcggccggg gtccaggaaa
     4741 ggatgcgatg agaggtcatc tttcttcttt ccgttctgga gtgcccggaa agcggcagat
     4801 cttgacgtcg cgacggtgcg gggcgcggtc ggcgtgccgg ttcatccgct gtcgggcagg
     4861 tgcgattccc ggcgggcgca gccttgtagg aagaggcttt ctcgggcgac cgccgaagaa
     4921 cataacgatc gctacgttag aaacgcggag ttggtgtagt caagggaatc tatttagttg
     4981 ctatcgtgtg tttagaggtc ttatcggtat tggagggttc cgaaggcttt ctgttcgccc
     5041 ccctgaaata cgcggaaaac gcaggtcccg tgatgtggca cgcacaggac gcgggcgggc
     5101 ggactgaatt cggtgaaccg caccgctctc agccgtccgg ccgtggggcg cagcagggtg
     5161 ccgtgactct ccagggccgc ggcctgccgg gccgcttcca gcagcaccat gcccggcaca
     5221 tgatccgtgg cgtggtcgaa gaagaacgga tgcttcggat cgaccggttc caccaggagg
     5281 gtgccgccgt cgagcccgat cacgatgtcg cgcggatcgg ccaccgccgg tccggtggcc
     5341 tccgggcgca cccggacagc cgttcgcgag gtgcgccgga cggtggtcac ggccccgcgc
     5401 agcacggtgt accggcgcgc gtcgagaaag cgcgcgccgc ccccggcgcg ggcgaaggcc
     5461 gtaccgccgg ccaggaaggt cacctccagc cggagcccgg tgacggcgcc gtcggccccg
     5521 gtgcgtatct cgctcacacc ggtcaggcag gtgacatcgg tggcggcgga agcggcgcgc
     5581 ggttccgccg ccggggtgag ggcgaagaac agatcggtga tgagcagatg cgcggtcgcg
     5641 ggcacgccga aataccgcag cgggagatag atgccgagct ggcgcagggt ttccacgacg
     5701 atcagcggac tgtgcaggtc cgcaccgtcc cgggggaatg tgggatggga ccggggccag
     5761 gacgccgcgg cctcgaagct gccgtcaccg gtccgccgta cgtcggtgag gagtacctcc
     5821 gccaaggagg tgcggtgcac catttcgcgt gcgacggtgc gtgaccagct cagcggccgg
     5881 agttccccgg cggtctcgcc gaccgaagtg ttcatgcaat aagaagaacc cagttccggt
     5941 gtccgcgcac gcccgtcccg tcgcgggtgt gagggcgagc cactacattg cctgctgtcg
     6001 gcgaccgagc ggtatccggg gtgaatgtgc aggtacgggc gaggaccacg cgaaggtctc
     6061 tgctggagac ggcggcgcga ctgttcgacg agcgggggta tgccggcacc agtatcagcg
     6121 atatcagtac ccggtccggc cgtaccagcg gggccatcta tttccattac gccagcaagg
     6181 agaatctggc gctcgccatt gtcgaggagc atttcgcgac ctggccggag ctgatcgccc
     6241 gccaccgggc ggcccgcctg cccgcgctgg acacgctggt cgcgctgagc ttcgatgtgg
     6301 cccgcgcctt ccgcgacgat gtcgtcatcc gggccggctc ccgcctctgg atggagcgca
     6361 aggccatcga cgccgcactg ccgacaccgt tcgtcggctg gaccgctacc gtcaccgaac
     6421 tcctcgaaga ggcccgcgcg gagggcacgc tcgccgccgc gatcgacccc ggaacggccg
     6481 cgcacggcgt cgtctgcgcc ttcttcgggc tgcacaccgt cgccgacgcg ctggacggcc
     6541 gcagctgcat cgaggagcgg ctgcacggcc tgtggctgct gctcctggcc gcgctccagg
     6601 ccgaccccgc gccagccgcg ctgctcagcc gcgttcacga ccgggcgggg gccgccgagc
     6661 agcgccggcc cgcccgcccg gccgccaccg gaccggcccg gagcggcctc gcggccgtcg
     6721 gcgccgcgcg ggcgaagtag ccccggccgt cactacgggg tgaagtggtc ccgcccgccg
     6781 cgacggaggg acgggggagg agagcgcagc gggaacgttc cggtatttct ccgccacggg
     6841 ccccgtgcgg agaagcggcc ggcaggagga aagccggggg aatcccggga aagggccggc
     6901 gttcacccac cgtttcccgt acggatatcg agatttcggc attccggcat tttggaattt
     6961 cggatgtctg ccgtttcgtg gatctccggg gttcggagca ccggcgagcc gaggagccga
     7021 aggcagggga atggcgggag cgccgccgca cggcgcattc gcgggcgcgg ggttcacccg
     7081 gacggccgac cggcggcgcg gcggccgcgt cggaccgggt ccggccgggt gacctgcggt
     7141 gccgttcgcg gggctgcgta tgatcgcgaa cgcggggcgc tgtggggacg actgatgggg
     7201 tgtggacgtg cgaaaccgcc aactgccgtt gcagcgtggg gtcacggtgg cgatcggcgc
     7261 tctcgggtgc acggtactgc tggccggggg gacggccggg gcccggccgg tggcgggtgc
     7321 gccggtggcc cgggtcgcgg cggcgggggg cgggacggtg ctgccgcaga tcccggacgg
     7381 gccggtcgcg gagggcaagt gcaccaagga gtcggcgcgc acgatctccc agaccccctg
     7441 ggcccagaag gcgctcggcc tgtcggaggt acggccgctg agtcagggcg gcggggtgac
     7501 cgtcggcgtc gtggacaccg gtctcgacgc gggcgtcgcg gcgctcaagg gacgcgcgtc
     7561 gggcggcggg ggcaaggact gcgcggggca cggcacgttc gtcgcgggga tcgtggcggg
     7621 ggccgcgcag gacggtgtcg ggttcgccgg cgtcgcgccg cgggcccggg tgctggggga
     7681 gaccgtgagc ggtgcggacg ccggggcgct ggccggcggt atcgaggacg cggtggccgg
     7741 cggcgccaag gttgtgctgg tcgcggcgag cgcgacggag tccgacgcgc ggttgcagac
     7801 ggcggtcgac aaggccgcgg ccaaggacgt gctcgtgatc gccggggcca cggccgggag
     7861 caagggtgcg caggcgtacc cggggggact gtccggggtg ctcagcgtgg cggccgtggg
     7921 ggtcgacggg aaaccgtatc cgggccgtac gggcgggcag tccaccggtg cgtcgctgcc
     7981 gcgggtggac ctggcggcgc cgggggaccg ggtggtcagc gtgggtcccg gcggcggcca
     8041 cttcaccggc ggcggggact cggtggcggc ggccttcgtc gcgggcaccg ccgcactggt
     8101 gcgcgcgcac gaccccgggc tgtccgcggc ccaggtcgcc gaccggctgc gggcgacggc
     8161 ggcgcgcggg cccggggcgg tcccggacgc ccggttcggg tacggactgg tggacccgcc
     8221 cggcgcggtg ggcgcggtct ccgtcgccgc gtccggcacg ggcgcgcgga cccgtacgga
     8281 gagcgccccc gcgctggacg ggacctaccg ggtgccggag cagccgcctg ccacctcgcc
     8341 ggccgtggcc tgggcggtcg tgggtgttgc cgccctcgta tccctgctgg tgggcggcgc
     8401 ggctctggtg atcccgcgcg gccgggcgcg cggttggcgc cccggccgcc tcgggtaggg
     8461 ggcctcccgc ccctcagtcg tcctcggcca gctgcgccgt ctggacctgg atctggcgcc
     8521 tgcgggcgac ccacagggcg cggcccggcg ggtactcgcg gggcttgacg ttgccgagga
     8581 tcaggccctc ggaggccggg caggagaaca cgacgctcgg ggtgttcgcc tccaccaggc
     8641 ggcgcagcag cggatcgctc agggcgcggc cgatgccgga ggacgaccgg gagacgatca
     8701 ggtgcaggcc cagctcggcg ccctggctga ggagttcgag caccgggccg aaggggctct
     8761 cggctccgta gccgctgccg gagaccaggt cgtagtcgtc gacgaggagg aagacctgcg
     8821 ggccgtccca ccagtcgcgc ttgcgcaggc ggtccggggt gatctcgggg ccgggtatcc
     8881 gcggctgcat cgccccggcg gcggccagca tcatctcgcg ggtggcgtcc gcggagaccg
     8941 cgtagcccag gcggtactcc tcggggaccg cgtcgtacag ctcgcggcgg acgtcgacca
     9001 tggcgatcag cgcctcctcg ctggtgtgcg tggcgacgat cgagcgggcg atgtgccgca
     9061 ggaggttggt cttgccgctc tccgtgtcgc cgatgaccag caggtgcggt gacgcgtcga
     9121 agtcgtgcca gaagggcgcg agttcgttct cctccacacc gagcgggacc cggcgcccct
     9181 cggcggcggg cagctcggtc gcggggaagg cggcggggag catccgcacg gccggggcgc
     9241 ccggtccgtc ccacgcctcg cggacggcgg cgaccagggc cttggtgccg tcggacaggt
     9301 cggcgaccgc gccgcttccg tccatgcggg ggagtgcggt caggaagtgg taggagtcct
     9361 cggacaggcc gcggcccgcg acgcgcggca cctcctgtgc cttgcgcatg ccgatgaggg
     9421 agtcgaccgg gtcgcccagg cgcagctcga acttggtgcc gatctggtcg cgcagcgccg
     9481 gctgcatctc ggcccagcgc ggggacgcca cgatgaggtg gatgccgtag ttgaggccgc
     9541 gggcggccag ctggacgacg gtctgggtga ggtcgtagaa gtcctcgcgc agggtggacc
     9601 agccgtcgat gacgaggaag gcgtccccga agggctcctc ggcgaactcg cccgccgcgc
     9661 gccgggtgcg gtagtcggcc atggagtcca cgccctgctc ggcgaaggcc agttcccggc
     9721 gggcgagcag tgaggtgatc tcgccgatgg tgcgggccag ccgctcgggg tgcagccgtc
     9781 cggtgaccgt gccgacgtgc ggcaggtcgg tcagcgaggt cagcgtgccg ccgccgaagt
     9841 ccagacagta gaactgcact tcgcgcgggg tgtgggtgag ggccagggcg gtgatcaggg
     9901 tgcgcagcag ggtgctcttg ccgctctgcg gaccgcccgc gatgcccaca tggccgccgg
     9961 cgccgccgag gtcggccagc agcgggtcgc gacgctggtc gaaggggcgg tcgatgacgc
    10021 cgacgggcac cttcaggccg ccgcggacgg tgccgtaccc gtcgagccgg acggtgccgg
    10081 gcgcaaggag ttcgtccagc gacggcgggg tgcccagcgg gggcagccag atggggtggg
    10141 cctcggggcc cgcctcgtgc agccgctcga tggcgacctg gagcaggctc gccgactcgg
    10201 tctcctcctc cgcgggccgc gggagatcgg gcacgacggg gtgggcgggc ggtacgtaac
    10261 cggagcggta gggcaccacg gcgccggtgc cggtcgccgc ctggggggtg cggcgcttgg
    10321 cccggtaggc gccggagacg tacgcggcct tgaagcgggt gagcgtggcc tggtcgctct
    10381 tgaggtagcc gttgccgggg gcggacggca gctggtgggc gtcggggacg ccgaggacgc
    10441 cgcggctctc catcgcggag aacgtccgca ggccgatgcg gtaggaaagg tgcgattcca
    10501 gctggtgcac ccggccctcg tccaggcgct gggaggccag cagcagatgg acgcccaggc
    10561 tgcggcccag ccgtccgatc atcacgaaca ggtccatgaa gtcgcggtgc gcggcgagca
    10621 gttcgctgaa ctcgtcgacg acgacgaaca gggacggcag cggtgccagc gcggcgccgg
    10681 acgtccgggc cttctcgtag tcgagtacgg agctgaagtt gccggcgctg cgcagcagtt
    10741 cctgacggcg gatcagttcg ccgtgcaggg cgtcctgcat acgggtcacc agcgcggcct
    10801 cgtcggcgag gttggtgatg acggcggagg tgtgcggcag ctgctccatg cccaggaagg
    10861 tggcgccgcc cttgaagtcg accaggacga ggttgagcac ctcggaggag tgggt
//
