LOCUS       NZ_NDXL01000001        11381 bp    DNA     linear   CON 27-APR-2024
DEFINITION  Streptomyces kasugaensis strain BCRC 12349 scaffold1, whole genome
            shotgun sequence.
ACCESSION   NZ_NDXL01000001
VERSION     NZ_NDXL01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces kasugaensis
  ORGANISM  Streptomyces kasugaensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Comparative genome analysis for kasugamycin overproduction
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Comparative genome analysis for kasugamycin overproduction
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030,
            China
REFERENCE   4  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. May-2015
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 188.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002261115.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 02:42:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,951
            CDSs (total)                      :: 7,863
            Genes (coding)                    :: 7,657
            CDSs (with protein)               :: 7,657
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 4, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 4, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 87 of 206
            Pseudo Genes (incomplete)         :: 170 of 206
            Pseudo Genes (internal stop)      :: 23 of 206
            Pseudo Genes (multiple problems)  :: 65 of 206
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:37:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3531073
            Orig. end                         :: 3542454
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NDXL01000001.1.
            Bacteria and source DNA available from BCRC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11381
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="22"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11381
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11381
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6381](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6381)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(1174..1189)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.61"
                     /tool="antismash"
     gene            1187..1915
                     /gene="hemQ"
                     /locus_tag="B7024_RS15160"
     CDS             1187..1915
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981978.1"
                     /locus_tag="B7024_RS15160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_052856798.1"
                     /transl_table=11
                     /translation="MTDASAPAPEKIPNAGKKAKDLNDVIRYTLWSVFRLRDVLPEDRS
                     GYADEVEELFDQLAAKDVVVRGTYDVSGLRADADLMIWWHAETADALQEAYNLFRRTRL
                     GRALAPVWSNMALHRPAEFNKSHIPAFLADENPRAYVSVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGFPDVRANTVPAFSLGDYEWILAFEADELHRIVDLMRHLRASEARLHVRE
                     EIPFYTGRRKPVAELVAGLA"
     gene            complement(1995..3728)
                     /locus_tag="B7024_RS15165"
     CDS             complement(1995..3728)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (91-290). E-value:
                     3.2e-18. Score: 58.1. Matches aSDomain:
                     nrpspksdomains_B7024_RS15165_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602445.1"
                     /locus_tag="B7024_RS15165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_244933939.1"
                     /sec_met_domain="PF00561 (E-value: 1e-24, bitscore: 85.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALALYGTIGSLILTALTTAPAGGATAPSAAPPAPVRPIVFGRC
                     SAVEHLSSSVECGKLTVPLDYDRPDGKKISLTVSRSRATSPEERQGALVFNPGGPGASS
                     MEFPLYGALAKWRKTARSYDFVGYAPRGVGRSAPLSCQDPKKFAKSPTDSPRYPGEEYK
                     REKLAEAEEYARGCARNAGADLPFYNSVNNARDLDMLRAALGEEKLTFMGASYGTYFGA
                     LYATLFPGHVRRMIFDSVVNPDPDHVWYRNNLDQSIAFERRWNDWARWVAAHHAVYRLG
                     TTVAQVRRAYDTVAEKLRRAPLNGKIGPGQLQGAFLKTGYNDAYWPSRAAALSAYLHGD
                     PQPLITQASPHPENAKAAENGNAVYTAVECNDAAWPRDWRTWDRDNTEAARLAPFETWD
                     NVWMNLPCAFWPDRSESAAAGIGAVAGEAADRAAARDGRAGHDGHDAAADATGEQLAAA
                     AGHATATGIDSALNPRRPLDVRTAPGALPPVLLLAAERDAATPYPGALELQRRLPGASL
                     VTENGAGTHGIAFGDNDCVNRYVEAYLLDGKVPGERVYCAARPEPVADAHAHAQHKPKK
                     K"
     aSDomain        complement(2859..3455)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B7024_RS15165_Abhydrolase_1.1"
                     /evalue="3.20E-18"
                     /label="B7024_RS15165_Abhydrolase_1.1"
                     /locus_tag="B7024_RS15165"
                     /protein_end="290"
                     /protein_start="91"
                     /score="58.1"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASSMEFPLYGALAKWRKTARSYDFVGYAPRGVGRS
                     APLSCQDPKKFAKSPTDSPRYPGEEYKREKLAEAEEYARGCARNAGADLPFYNSVNNAR
                     DLDMLRAALGEEKLTFMGASYGTYFGALYATLFPGHVRRMIFDSVVNPDPDHVWYRNNL
                     DQSIAFERRWNDWARWVAAHHAVYRLGTTVAQVRRA"
     misc_feature    3822..3837
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.49"
                     /tool="antismash"
     gene            complement(3968..4996)
                     /locus_tag="B7024_RS15170"
     CDS             complement(3968..4996)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055541.1"
                     /locus_tag="B7024_RS15170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_244933940.1"
                     /transl_table=11
                     /translation="MSLAQSMYLAVILSSLVLVIGTVRSRRAPRIRLVDAPTHDVWEIA
                     FLAGGPGRVTDAAIAGMQEDGRLAVGGPGAVTVRRAVAHHPVEQAVLDALARTPGGALS
                     RLRGMVMRSPAVQEIGDRLADRGLMRRPRRGRGWRRMAHVQVAASAVLLLGGLLMTITG
                     TAGDDAFTVPFVVRIAPAAILGIVVGSLCKLAHRSRLTPAGALALRAAHAANSPGVVGG
                     AVRSAAIVVALGGAALVADAVLRQQLLDAQSAMATAAGVSAASGDTGASSTTTSSGDSG
                     WDFSDGGSDVSWCGSADGGSGCGSSGCGGSSGCGGGSSCSSGSSCSGGSSCGSSCGSSS
                     SD"
     gene            complement(5001..6381)
                     /locus_tag="B7024_RS43350"
                     /pseudo=""
     CDS             complement(5001..6381)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944755.1"
                     /locus_tag="B7024_RS43350"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 8.8e-93, bitscore: 308.4,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQRLGTGIGWRPEIAAEIAGLSGVDWVEVVAENICPGHLPPALRE
                     LRERGTAVVPHGVSLGLGGAERPAEGRLTALAERAVALDAPLVTEHIAFVRAGGPGTGS
                     PLLEAGHLLPVPRTLDALDVLCENVRIAQDALPVPLALENIAALLSWPGEELTEGQFLS
                     ELVERTGVRLLIDVANLHTNHVNQGEDPAVALAELPVEAIAYVHVAGGVERDGVWHDSH
                     AHPVTRPVLDVLAELCARTTPPGVLLERDEDFPEGAELAGELDAIRTVVEEARHGRRAV
                     "
     gene            complement(6531..7202)
                     /locus_tag="B7024_RS15180"
     CDS             complement(6531..7202)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578488.1"
                     /locus_tag="B7024_RS15180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LysE family translocator"
                     /protein_id="WP_052856676.1"
                     /transl_table=11
                     /translation="MLTSLLGFTAVAALLTVSPGPDFAVVLRTALGSGRRAALCSALGI
                     AAGCFVWGLAGAVGLTAMLSASQVAYDALRVAGALYLMWLGVQALRAARRLRTAPAADG
                     AVAADGAEPTAADAGGVRQTPLRAFRTGALTNVLNPKVGVVYMSLLPQFIPHGAPVIAT
                     TLLLVAVHAALGVLWLGTIAVAVHRARAVFQRPRVRRRLDQATGGVLLALGAAVGFEAA
                     R"
     gene            complement(7482..8327)
                     /locus_tag="B7024_RS15185"
     CDS             complement(7482..8327)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032960.1"
                     /locus_tag="B7024_RS15185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="arginase family protein"
                     /protein_id="WP_094792250.1"
                     /transl_table=11
                     /translation="MSGPIVFEVAQWQGSGAATARRLHSGAAWLAELVPASRRHTVALD
                     SGVGGARGGVRNAEVLERHLRAVREALAEVPPGEPTVTIGGDCGVDLAPVETALAAFGD
                     RLAVVWFDAHADLNTPESSPSGAFHGMVLRALLGDGPEGLRPVRNVSADRVVLAGARSL
                     DAGEQALVESSGIRHLGVGQLAEPGPLLAAVRATGAEAVYIHIDLDVLDPRVFPAVGTP
                     EPGGLGVAELVAAVRALTDEFTLAGLALTEYERTEAADQEEAVLRRIVDGLFGPDRHEH
                     "
     gene            complement(8747..9691)
                     /locus_tag="B7024_RS15190"
     CDS             complement(8747..9691)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /GO_process="GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /locus_tag="B7024_RS15190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="WP_172450672.1"
                     /transl_table=11
                     /translation="MRPFRFLATVNDNRRFDGLTSLARKAEAVGCAAFVLPDHLTAYAP
                     IPPLAMVAAATERLRVGTFVLNIGLRHPAVLAQDLATLDALSGGRLEIGLGAGWNKPEH
                     DAIGIPFEPVGVRIKRLTEGIAILKGCFAEGPFSFSGEHYTVTDHVGAPAPAQRPHPPF
                     FLGGGGKRLLTLAAREAQIVGLAPRLVQGDRPGLEARSMTAAATEEKVGWIREAAGDRF
                     DGLELNTYPSGGPTVVTGDPRAAARRRADHLREQTGVELTVEEILDSPHMFIGSVKDLT
                     RKFVELRERFGISSFLIDDLDALAPVVEELAGR"
     gene            complement(10013..11113)
                     /locus_tag="B7024_RS15195"
     CDS             complement(10013..11113)
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578150.1"
                     /locus_tag="B7024_RS15195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_094792252.1"
                     /transl_table=11
                     /translation="MPAVVAVAAGVMTMGALAPPAASAAARPDSVQQGLNALVHADGMP
                     AALASVQDRGGRTRTYTAGVGDLATGSKVPTDGQVRIGSNTKVFTAVVMLQLVGERKIR
                     LDAYVDTYLPGLVRGDGIDGRRITVRHLLQQTSGLPNYTEYDLQLRHYEPRELLGIALR
                     HKAHFDPGKKWEYSNTNYVLAGLIIQKVTGRPVAKEIDQRIIKPVGLRHTYFPAPGDTT
                     IREPHPKGYDRDSADAPPRDITEMDPSWAWAAGQMISTNSDLNRFFTALLGGRLLPPAQ
                     LAQMRTTVPADYFGDGARYGLGLVSKPLSCGGVYWGHGGSFPGYETRGGVTAKGRAANI
                     AVTIQPADKTAMERVEKVVDTALCAR"
     misc_feature    complement(11146..11166)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.2"
                     /tool="antismash"
ORIGIN
        1 ccaccgccgc cctgtggacc cgcggcgcgc tgcgccccat gcccaagggc catgtgatgg
       61 gcgtccccgg cgatctcggc ccgctcgccg attccggtgt gctctccccg gccggtctgg
      121 cccggatcgc cgaggacgag acgctggcgc ggaccgaggc cggcgaggac gtcgccgtcg
      181 gcgagtacgt cgcggcccgg ctcggccgcg aggtcgtcga ccggctggtg gaaccgctgc
      241 tcggcggcgt ctacgcgggc gacgcctacc gcatctcgat gcgcgccgcc gtcccccagc
      301 tcttcgacgc cgcccgcacc gagcgctcgc tgctccaggg cgtacgggcc ctccaggcgc
      361 gcaccgccgc cacgacgccg cccggcggac cggtcttcac gggcctcgac ggcggcatcg
      421 gaacgctgcc ggtcgccgtc gccgacaccg tgcgcggcgc cggcggcgag atcctgctcc
      481 gcaccgccgt ggacgagctg acctggcgcg acgaccactg ggcggtgcgc gtgacggagc
      541 ggggcgccca ccgcgttctc acggcggacg cggtcgtgct ggccgccccc gcgggcgccg
      601 cggcccggct gctgcacgac ggcgccccgg ccgccgccgc cgagctggcg ggcatcgagt
      661 acgcctcgat ggcgctggtc acgatggcct tccggcgcgc cgacctggac cgggtgcccg
      721 gcggcagcgg cttcctcgta ccgcccgtcg acgggcaccg gatcaaggct tcgacgttcg
      781 ccagccacaa atggggctgg ctcgccgacg ccggcccgga cctcttcgtc ctgcgcacct
      841 ccctcgggcg ctacggcgac accgccgacc tcgggcgcga cgacgccgag ctggtcggcc
      901 tctcgctcgc cgacctcgga gccgcggtgg gcctggccgc gcgcccggtg gcgagcacgg
      961 tcacccgctg ggacggcggt ctgccgcagt acacggtcgg ccatctggac cgggtcgccc
     1021 ggatccgtac cgcggtggcg cagctgccgg gtgccctgcg ggtgtgcggc gcggtctacg
     1081 acggcgtcgg catcccggcc tgcatcggct cggcacagcg ggccgccgac gaggtcctcg
     1141 gcaccctgcc gcaggccacc gcccccggcg agtgagaatg gatgacatga cagacgcttc
     1201 cgcccccgca cccgagaaga tcccgaacgc cggcaagaag gcgaaggacc tcaacgacgt
     1261 catccgctac accctctggt ccgtgttcag gctgcgcgac gtcctcccgg aggaccgcag
     1321 cggctacgcc gacgaggtgg aggagctgtt cgaccagctc gccgccaagg acgtcgtggt
     1381 gcgcggcacc tacgacgtgt ccggcctgcg cgccgacgcc gacctgatga tctggtggca
     1441 cgcggagacc gcggacgccc ttcaggaggc gtacaacctc ttccgccgca cccgcctggg
     1501 ccgcgccctc gcgccggtgt ggtcgaacat ggcgctgcac cgccccgccg agttcaacaa
     1561 gtcgcacatc ccggccttcc tggccgacga gaacccccgc gcctatgtga gcgtctaccc
     1621 cttcgtgcgc tcctacgact ggtacctgct gcccgacgag gaccgccgcc ggatgctcgc
     1681 cgaccacggc aagatggccc gcggcttccc ggacgtgcgc gccaacaccg tgccggcgtt
     1741 ctcgctcggc gactacgagt ggatcctcgc gttcgaggcc gatgagctgc accgcatcgt
     1801 cgacctgatg cgtcatctgc gcgcctccga ggcgcgcctg cacgtccgcg aggagatccc
     1861 cttctacacc ggccgccgca agccggtcgc cgaactggtc gccggtctgg cctgacgcgg
     1921 acgcggcagg ggccgtcccc gggaacacca ccggtgtccc ccggggacgg ccccttcccc
     1981 tgtccgcggc cgcgctactt cttcttcggc ttgtgctgcg cgtgcgcgtg cgcgtccgcc
     2041 accggctccg ggcgggccgc gcagtacacg cgctccccgg ggaccttgcc gtccagcaga
     2101 tacgcctcga cgtacctgtt cacgcagtcg ttgtcgccga acgcgatgcc gtgcgtgccc
     2161 gcgccgttct cggtgaccag ggaggcgccg ggcagccgcc gctggagctc cagcgcaccc
     2221 gggtacggcg tcgccgcgtc ccgctcggcg gccagcagca gcaccggggg cagcgcgccg
     2281 ggcgcggtac ggacatcgag cgggcggcgc gggttcagcg cactgtcgat accggtcgcg
     2341 gtggcatggc cggcggcagc ggccagctgc tccccggtgg cgtccgcggc tgcatcgtgc
     2401 ccgtcgtgcc cggcacgccc gtcgcgcgcg gcggcgcggt ccgccgcctc gccggccacg
     2461 gcgccgatgc cggcggcggc cgactccgac cggtcgggcc agaaggcgca cggcaggttc
     2521 atccagacgt tgtcccaggt ctcgaacggc gccagccgcg cggcctcggt gttgtcccgg
     2581 tcccaggtcc gccagtcccg cggccaggcc gcgtcattgc actcgacggc ggtgtagacg
     2641 gcgttgccgt tctccgcggc cttggcgttc tcgggatgcg gcgacgcctg ggtgatcagg
     2701 ggctgcggat caccgtgcag atacgccgag agcgccgcgg cgcggctcgg ccagtaggcg
     2761 tcgttgtagc cggtcttgag gaacgccccc tgaagctggc cggggccgat cttgccgttc
     2821 aggggcgccc gccggagctt ctcggcgacc gtgtcgtacg cgcgccgcac ctgtgccacg
     2881 gtcgtgccga gacggtagac ggcatggtgt gcggcgaccc accgggccca gtcgttccag
     2941 cgccgctcga aggcgatgct ctggtcgagg ttgttgcggt accagacgtg gtcggggtcg
     3001 gggttgacca cactgtcgaa gatcatccgg cggacgtggc cggggaagag cgtcgcgtag
     3061 agggcaccga agtacgtgcc gtacgacgcg cccatgaacg tcagcttctc ctcgcccagc
     3121 gcggcccgca gcatgtccag gtcgcgggcg ttgttgaccg agttgtagaa gggcagatcg
     3181 gcgccggcgt tgcgggcgca gccgcgcgcg tactcctcgg cctccgccag cttctcgcgc
     3241 ttgtactcct cgccggggta ccgcggactg tcggtggggc tcttggcgaa cttcttcggg
     3301 tcctggcagg agagcggcgc cgaacggccg acgccgcgcg gcgcgtagcc gacgaagtcg
     3361 taggagcggg ccgtcttgcg ccacttggcg agcgcgccgt agagcgggaa ctccatgctg
     3421 ctcgcgccgg ggccgccggg gttgaagacc agggcgccct gccgctcctc gggggaggtg
     3481 gcgcgggagc ggctgacggt caggctgatc ttcttgccgt cggggcggtc gtagtcgagc
     3541 ggaacggtga gcttgccgca ctcgacggag gacgacaggt gttccaccgc cgagcaccgg
     3601 ccgaagacga tggggcggac gggggcgggc ggcgcggcgg acggtgcggt ggcaccgccc
     3661 gccggcgcgg tggtgagggc ggtcaggatc agcgacccga tggtgccgta caacgcgagt
     3721 gctctcacga gccgtccctt cgtatggtgc gttggcgcac cgcgagaggc ccggggcgcc
     3781 tggccgcacg gtgtacatac aaaagggata gttggggcgg tgattgccga tgtaaagcac
     3841 cggccgccgg tgtcggacgc tatgccccgg aagagtggag cgcgcccgaa accccggacg
     3901 ccacgtcagg ccaggggcta cgcaacgccg ttcgggcggc ccgcctccgg ccgcccggcg
     3961 catggcgtca gtcgctgctg ctgctcccgc acgacgagcc gcagctggac ccgccgctgc
     4021 aactcgaccc actgctgcaa ctggacccgc caccgcatcc cgagctgccg ccgcacccgg
     4081 agctgccgca gccggacccg ccgtcggcgg agccgcacca ggagacgtcc gatccaccgt
     4141 ccgagaagtc ccagccggag tcgcccgagg acgtggtggt ggacgaggcg ccggtgtcgc
     4201 ccgacgcggc ggacacgccg gcggcggtcg ccatggcgga ctgcgcgtcg agcagctgct
     4261 gtcgcagcac cgcgtcggcc accagcgccg caccgccgag cgccaccacg atcgcggcgg
     4321 accggacggc gccgccgacg acaccggggc tgttggccgc gtgcgcggcc cggagcgcga
     4381 gggcgcccgc gggggtgagg cggctgcggt gggccagctt gcagagggag ccgacgacga
     4441 tgcccaggat ggccgcgggc gcgatccgca ccacgaacgg caccgtgaac gcgtcgtccc
     4501 cggcggtccc cgtgatcgtc atcagcagac cgccgaggag cagcaccgcg gaggccgcca
     4561 cctggacgtg ggccatccgg cgccagcccc ggccccgccg cggacgccgc atcagaccgc
     4621 ggtccgccag ccggtcgccg atctcctgca ccgccgggct ccgcatcacc atgccgcgca
     4681 gcctgctcag cgccccgccg ggggtccggg cgagcgcgtc cagtacggcc tgctcgacgg
     4741 ggtggtgcgc gacggcccgg cgtacggtca ccgcgccggg gccgccgacg gccagccggc
     4801 cgtcctcctg cataccggcg atggcggcgt ccgtcacccg ccccggcccg ccggccagga
     4861 acgctatctc ccacacatcg tgggtggggg cgtccaccag ccggatgcgc ggtgcccggc
     4921 gggagcggac cgtaccgatg acgagcacca gcgaggagag gatgactgcc agatacatcg
     4981 attgtgccag ggacacggtg tcaccttcgg tgcagggcga gccgcacggc tcgcacgagt
     5041 cgggcggcgg ggcgggcggg cggcggcttc ggccccgccc ggtcccgcca ccaccgtgtc
     5101 aactgccgcc gcgcggccgg atccgcggga cgctcctggg ccagcagatg cgccgcgaag
     5161 tccagcgcgt cctgccggtg gccgccgtgg aggggccggt ggcgggcgta gtcgaggaag
     5221 gcgggccggt actcctcgcc gaggatagcg ggcagctccg gggcgatcct gcccacgacg
     5281 gcggcccgct tggcggccag cgcccggctc tggacgcgca gccggcgacg gtcgaacccc
     5341 tcgggggccg gcgtcccggc gacgagcgcg gacagcagcg cggtctgcgc gagcgccagc
     5401 cgctgccgcg ccgcgtcggt gctggtatcg gtatcggtac cgacggggta cggggggacc
     5461 gtgcgggcgg gcacgccggg accggccgcg gccgtgtccc cgccgcccgg accgccaccg
     5521 gcccccgtga ccgcctcaga ccgctcgtcg gccatggcgc gcctcctcca ccacggtacg
     5581 tatcgcgtcg agttcaccgg ccagctccgc gccctcgggg aagtcctcgt cgcgctccag
     5641 cagcacaccg ggcggggtgg tccgggcgca gagctcggcg agcacgtcca gcaccggccg
     5701 ggtcacgggg tgggcgtggc tgtcgtgcca caccccgtcc cgctccacac cgcccgcgac
     5761 atggacatag gcgatggcct cgaccggcag ctcggccagt gcgacggccg ggtcctcacc
     5821 ctggttgacg tggttcgtat ggaggttggc gacatcgatc agcagccgta cgcccgtccg
     5881 ctcgacgagt tcggacagga actgcccctc ggtcagctcc tcaccgggcc aggagaggag
     5941 ggcggcgatg ttctccaggg ccagcggcac cggcagcgcg tcctgggcga tccggacgtt
     6001 ctcgcacagc acgtccagcg cgtcgagggt acgcggcacc ggcagcagat gcccggcctc
     6061 cagcagcggc gacccggtgc ccggcccgcc ggcgcggacg aacgcgatgt gctcggtgac
     6121 cagcggcgcg tccagcgcca cggcgcgttc ggcgagcgcg gtcagccggc cctcggcggg
     6181 ccgttcggcg ccgccgaggc cgagcgagac accgtgcggg acgacggcgg tgccccgctc
     6241 gcgcaactcc cgcaaggcgg gcgggagatg gccggggcag atgttctcgg ccaccacctc
     6301 gacccagtcc acaccggaca gtccggcgat ctccgcggcg atctccggcc gccagccgat
     6361 acccgtaccg agacgttgca tcgttccccc cgttcactcc gtgctgtgac gtactcatga
     6421 ccctgtcaag acgggccgaa tcctgcgccg tcagggttca gagcttgctc tgagcttccc
     6481 gccgccccgt acggctccgc cgccggatct ccgtacggcc ccgcgccgga tcagcgcgcg
     6541 gcctcgaatc ccaccgccgc gcccagcgcg agcagcaccc cgcccgtggc ctgatccagc
     6601 cgccgccgta cgcgcggacg ctggaagacg gcccgcgccc ggtggaccgc cacggcgatg
     6661 gtgcccagcc acagcacacc cagcgcggca tggacggcca ccagcagcag cgtcgtcgcg
     6721 atgaccggcg cgccatgtgg gatgaactgc ggcagcagcg acatatagac caccccgacc
     6781 ttggggttga gcacattggt cagcgcgccg gtccggaacg cccgcagtgg cgtctgccgt
     6841 acgccgccgg cgtccgccgc cgtcggctcc gccccatccg ccgctaccgc cccgtccgcc
     6901 gccggagcgg tccgcaggcg ccgggcagcg cgcagcgcct gcaccccgag ccacatcaga
     6961 tacagcgcac cggccacccg cagcgcgtcg tacgccacct gcgacgcgga gagcatcgcc
     7021 gtcagcccga cggcaccggc cagcccccag acgaagcacc cggccgctat gccgagcgca
     7081 ctgcacaggg cggcccgccg ccccgaaccg agagcggtcc gcaggaccac cgcgaaatcg
     7141 gggccggggg agacggtcag cagcgcggcc accgcagtga agccgaggag agaggtcagc
     7201 acgggacacc accgtacgtc caggactccg ggcggtcgca ggggggcccg gccgcacgtc
     7261 cggcggccgg gggcgggtac ccgcaccgcc cacggcgggg gagccggatg ccccgtcggc
     7321 accgcccgcg cgcggcggac ggtgccggac gcggcagtgc gcaccgccta tcgcctatcg
     7381 cgcatcgcgg gggcggacgg ccggcgacgg ccggacacgc gggtgtcggc cggacccgag
     7441 ggtgtcggcc ctgacggcga gcgccggtgc gatggcgccg ctcagtgctc gtgccggtcg
     7501 ggcccgaaca ggccgtcgac gatccgccgc agtaccgcct cctcctggtc ggcggcctcg
     7561 gtccgctcgt actcggtgag cgcgaggccg gcgagggtga actcgtccgt cagcgcccgc
     7621 accgccgcga ccagctccgc cacgccgagt ccgcccggct cgggggtgcc gacggccggg
     7681 aagacccgcg ggtcgaggac gtcgaggtcg atgtggatgt acaccgcctc ggcaccggtg
     7741 gcgcgcaccg cggccaggag cggccccggt tccgccagct gcccgacgcc caggtgccgg
     7801 atgccgctgc tctcgacaag ggcctgttcc cccgcgtcca gtgaccgcgc gcccgccagc
     7861 acgacgcggt cggccgacac gttccgcacg ggccgcaggc cctccggccc gtcgccgagg
     7921 agcgcgcgca gcaccatgcc gtggaaggcg ccggacggcg acgactccgg ggtgttgagg
     7981 tccgcgtgcg cgtcgaacca caccaccgcc agccggtccc cgaacgcggc gagcgcggtc
     8041 tcgaccgggg cgaggtcgac cccgcagtcg ccgccgatgg tcaccgtcgg ctcgcccggc
     8101 ggaacctcgg ccagcgcctc gcgcaccgcc cgcagatgcc gctccagcac ctcggcgttc
     8161 cgtacgccgc cgcgcgcgcc gccgacgccg ctgtcgagcg cgaccgtatg ccgccggctc
     8221 gcgggaacca gctcggcgag ccaggcggcc ccactgtgca acctacgggc ggtggcggca
     8281 cccgacccct gccactgcgc cacctcgaat acgatcggac cggacacgaa cgtctccctt
     8341 ccacgctgat cgacgcagcc tatgtcgtcg ccccagccgt cacacaagtc acatcgggga
     8401 tccgatggtg caggtcaccg gtgagcggcc gcgcccgcgc cagggccgga aggcgtcccg
     8461 ggccggcgga gcagcgggtc acctccgtgg tgggcgcggc ggagacccgg cacggtggtg
     8521 tcccttcggc ttccgcttcg ctcgccgccc ggttccggac ccgctcggca cacgtacccg
     8581 gacgccccca cgctcggccc gttaggcggc gacgtccggg catcgggcgt accacttcac
     8641 caccgccgtc acggacttcg cggccggacg gttcccctgg cccgcccgtc tcccgcagcc
     8701 gccgcgcacc caggtcttcg accggtccgc gcgtcctgcg gcgtcttcac cgtccggcga
     8761 gttcctccac caccggcgcg agcgcgtcca ggtcgtcgat gaggaacgag ctgatgccga
     8821 agcgctcgcg caactccacg aacttccgcg tcaggtcctt gacgctgccg atgaacatat
     8881 gcggcgaatc caggatctcc tcgacggtca gctcgacgcc cgtctgctcg cggaggtggt
     8941 cggcgcggcg gcgcgccgcc gcacgcggat cgccggtgac gaccgtcggc ccgccggagg
     9001 ggtaggtgtt gagctccaga ccgtcgaagc ggtcaccggc cgcctcgcgg atccagccga
     9061 ccttctcctc ggtcgccgcg gccgtcatgc tcctggcttc gagaccgggc cggtcgccct
     9121 gcaccagccg cggcgccaga ccgacgatct gggcttcgcg tgcggccagg gtcaacaggc
     9181 gcttgccgcc gccgcccagg aagaacggcg gatgcgggcg ctgtgccggt gcgggcgcgc
     9241 cgacgtggtc ggtgacggtg tagtgctcgc cggagaagga gaagggccct tcggcgaagc
     9301 agcccttgag gatcgcgatc ccttcggtca ggcgcttgat ccggacgccg accggctcga
     9361 aggggatgcc gatggcgtcg tgctcgggtt tgttccagcc ggcgccgagg ccgatctcca
     9421 gccggccgcc ggacagcgcg tcgagcgtgg cgagatcctg ggccagcacg gccggatggc
     9481 gcaggccgat gttgaggacg aaggtgccca cccgcagccg ctcggtggcc gccgcgacca
     9541 tggccagcgg cggtatcggg gcgtacgcgg tgaggtggtc gggcaggacg aacgcggcgc
     9601 agccgacggc ctcggccttg cgggccagag aggtcagtcc gtcgaagcgg cggttgtcgt
     9661 tgacggtcgc gaggaaacgg aaggggcgca tacgggcatc ctccgtgacc gtcggaggcc
     9721 gcggggtggt gtttggcgaa cgcccggatg gtggcggggt gttggcggtg gtcggcgatc
     9781 tcctcggtgc cggcccggag tactcctccc gccaccgcca ccgccaccgc cgtcgcccgc
     9841 ggcgccggca gttcgagcgc gccgttcccg aacgccgtga ccggcaggcg caccgccgac
     9901 gccgtggcgg catcggcggc agacccaccg gctgtgaccc ggcacaccat gccccgcgcc
     9961 gtaggtctcc ggctccgccc tgccgcgtcg gcaggtccct aaccggcgtg gttcagcggg
    10021 cgcacagggc cgtgtccacg accttctcga cacgctccat ggccgtcttg tcggccggct
    10081 ggatggtcac cgcgatgttg gcggcgcggc ccttggcggt gactccgccc cgggtctcgt
    10141 atcccgggaa gctgccgccg tggccccagt agacaccgcc gcacgacagc ggcttgctca
    10201 ccagccccag cccgtagcgg gcgccgtcgc cgaagtagtc ggcgggcacg gtggtacgca
    10261 tctgggcgag ctgggccggc ggaaggaggc ggccgccgag gagcgcggtg aagaaccggt
    10321 tgaggtcgga gttggtggag atcatctgac ctgccgccca ggcccaggag gggtccatct
    10381 ccgtgatgtc gcgcggcggc gcgtccgccg aatccctgtc gtagcccttg ggatggggtt
    10441 cccggatggt cgtgtcaccg ggggcgggga agtaggtgtg gcgcagcccg acgggtttga
    10501 tgatgcgctg gtcgatctcc ttggcgacgg gccggccggt gaccttctgg atgatcagac
    10561 cggccagcac gtagttcgtg ttgctgtact cccacttctt cccggggtcg aagtgggcct
    10621 tgtgccggag cgcgatgccg aggagttcgc ggggttcgta gtgccggagc tgaaggtcgt
    10681 attcggtgta gttgggcagt ccgctggtct gctggaggag atggcggacg gtgatgcggc
    10741 gtccgtcgat cccgtccccg cggacgaggc ccggcagata ggtgtcgacg taggcgtcga
    10801 gacggatctt ccgttcaccg accagttgca gcatgaccac cgcggtgaac accttggtgt
    10861 tgctgccgat ccgcacctgc ccgtccgtgg gcaccttcga gccggtggcc aggtcgccga
    10921 cgcccgcggt gtaggtacgg gtgcggcctc cgcggtcctg gacgctcgcc agtgcggcgg
    10981 gcatgccgtc ggcgtgcacc agcgcgttca ggccctgctg gacggagtcc ggccgggcgg
    11041 cggcggacgc cgcgggcggc gccagggcgc ccatcgtcat gacacccgcg gccaccgcca
    11101 ccacggccgg cacggctctg cgacggccgc cccgccgccc ggaagaacgg aacctctgcc
    11161 atgcctgctc acgcacgagc caactcctac ggaatcattg ggtaaccagc ggtatgtgct
    11221 ggacccagca tttccgtagg tgatcgttcg gggcgatcct gctacccgcc cggtgacggg
    11281 tagggctacc ccccggggcg cgctcagccg atggggaacg gggcggggac actctggccg
    11341 cggtggcggc gccaggagtg cgggcgcgac ggagccggtg g
//
