LOCUS       NZ_NDXL01000001        10816 bp    DNA     linear   CON 27-APR-2024
DEFINITION  Streptomyces kasugaensis strain BCRC 12349 scaffold1, whole genome
            shotgun sequence.
ACCESSION   NZ_NDXL01000001
VERSION     NZ_NDXL01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces kasugaensis
  ORGANISM  Streptomyces kasugaensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Comparative genome analysis for kasugamycin overproduction
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Comparative genome analysis for kasugamycin overproduction
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030,
            China
REFERENCE   4  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. May-2015
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 188.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002261115.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 02:42:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,951
            CDSs (total)                      :: 7,863
            Genes (coding)                    :: 7,657
            CDSs (with protein)               :: 7,657
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 4, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 4, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 87 of 206
            Pseudo Genes (incomplete)         :: 170 of 206
            Pseudo Genes (internal stop)      :: 23 of 206
            Pseudo Genes (multiple problems)  :: 65 of 206
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:37:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3321520
            Orig. end                         :: 3332336
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NDXL01000001.1.
            Bacteria and source DNA available from BCRC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10816
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="20"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10816
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10816
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5816](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5816
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(542..1198)
                     /locus_tag="B7024_RS14270"
     CDS             complement(542..1198)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551583.1"
                     /locus_tag="B7024_RS14270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="WP_052861915.1"
                     /transl_table=11
                     /translation="MDLFSRSWTALRTAVAQLPDEDFARPSGCAGWLVRDLVCHLIIDA
                     QDVLITLVTPAETEPTRDAVTYWDVAGTPPAGDDPLDALIVRLAAAYEEPSLLRFHLDD
                     VGSAAGRAAALADPGLRVGTRDVVLTAGDYLSAYVLEWTLHHLDLVAHLPDVAGPSEEA
                     LARTRDMLERIARAAFPASFSDKDALLVGTGRRAPTATEKAELGELAAKLPLVLG"
     gene            1400..3712
                     /locus_tag="B7024_RS14275"
     CDS             1400..3712
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1134: flavodoxin"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018524657.1"
                     /locus_tag="B7024_RS14275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="WP_094792146.1"
                     /transl_table=11
                     /translation="MSASRDEAGRLEPRVGPVPEGEPESHPYHHPAAGWGAAKSVSRFM
                     VREGALADGPRAILRMNHENGGFDCPGCAWPDDTKGLHLDICENGIKHVTWEMTRKRVG
                     REFFAGHSVTELSGWSDYALENQGRLTEPMVYDPGTDHYLPISWKDAFALVGRTLRELD
                     SPHQASFYTSGRLGNEATFLYQLMARELGTNNLPDCSNMCHEASGRALQASLGTGKGTV
                     DLKDWEATDALFILGVNAASNAPRMLTALAAAYRRGAQVVHINPLIEAAATRTIIPHDV
                     RSMATGHATPTSTLNLQPRIGGDLALLRGMAKAILEQATSDPRALDREFIDRHTTGFEE
                     YRALCEATPWREIEKQTEISRPDILKAARIYREADRSIVSWCLGISQHEHGVDTVREIV
                     NLLLLRGNLGREGAGPSPVRGHSNVQGNRTCGIDHRPGPEFLDRLAEVCGIEPPREHGL
                     DTVGTIQAMHRGEVKVFVGMGGNFALAAPDTPYTCEALRNCDLTVQVSTKLNRSHLVHG
                     RRALILPCLGRTEKDHQRTGVQSTSVEDSMSMVHLSVGMRRPASPHLLSEPAIIAGMAR
                     AALPDSATPWERYIEDYDRIRDTMAQVLDGFEDFNRRVRLPLGFRIKQPARELVFRTPS
                     GRAEFSTAPLPDVVPAPGTLTLGTLRSHDQWNTTIYSDNDRYRGIRRLRTLVFMNQDDM
                     RERGLSKFDPVDITSTAKDGTRRHLNGYLAVPHDIPRGCAAGYMPEMNVLCAIGDYSTQ
                     SDQPIMKHVKVTINPAT"
     gene            4000..4995
                     /locus_tag="B7024_RS14280"
     CDS             4000..4995
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018566630.1"
                     /locus_tag="B7024_RS14280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_094792147.1"
                     /transl_table=11
                     /translation="MVTTQAVVVPPSKAAVFLVVTLNPGGEAVAREALEELAGLIRSVG
                     FRAPDAALTGVVGIGAAAWPRLFDGPPPHDLHPFQPLTGPRHRAPATPGDLLFHVRARR
                     MDLCFELSRLIVERLGDAVTVVDEVHGFRYFDERDLMGFVDGSENPQGGAAAEAVFVGD
                     EDADFRGGSYVIVQKYVHDMTAWQSISVEEQERVIGRSKSANVELPDGVKPANSHVALN
                     TIVDDEGAERKIVRENMPFGTIGRGEFGTFFIGYARTPAVTERMLDNMFLGDPPGNTDR
                     ILDFSTAVTGGLFFAPSAGFLDSLPDPPAAARSGAVPSDGSLGIGGMKGA"
     gene            5001..5816
                     /locus_tag="B7024_RS14285"
     CDS             5001..5816
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017240918.1"
                     /locus_tag="B7024_RS14285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_094792148.1"
                     /sec_met_domain="Linocin_M18 (E-value: 1.3e-88, bitscore:
                     294.4, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPAATNNLHRELAPVTQAAWAQIEEEAGRTFRRHVAGRRVVDV
                     PEPAGPALAAVGTGHRTEIASPAPGVTACLREAKPLVELRVPFSVSRAAVDDVTRGSND
                     SDWQPVKDAARTMAFAEDRAIFDGYAAAGIDGLRDRTSNPVLSLPAEPRDYPDAISRAV
                     TSLRLAGVDGPYALLLGADEYRAVSETSDHGYPIAAHLARMLDGAPIWAPALSGAFVLS
                     TRGGDFELRLGQDVAIGYTSHDASSITLYFQETLTFLTDTDEAVVVLKH"
     gene            complement(6212..6706)
                     /locus_tag="B7024_RS14290"
     CDS             complement(6212..6706)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003952304.1"
                     /locus_tag="B7024_RS14290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GreA/GreB family elongation factor"
                     /protein_id="WP_094792149.1"
                     /transl_table=11
                     /translation="MAEGPEPISVDALRALRQELAELRAERGPVAATLRSEDPVGDRAD
                     EADALQRASEVARLDGRIAEIEGRIREASVAGPPPTDMVGVGSTVTVRFADGAEATVRI
                     GEIVEEPNRTLVTADSPLGRALLGRRTGDTVAYETPDGSTTAVVLSLGPGGAPPDDGPR
                     A"
     misc_feature    6802..6816
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.99"
                     /tool="antismash"
     misc_feature    6946..6959
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.71"
                     /tool="antismash"
     gene            complement(7143..8018)
                     /locus_tag="B7024_RS14295"
     CDS             complement(7143..8018)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (33-178). E-value:
                     7.5e-13. Score: 40.6. Matches aSDomain:
                     nrpspksdomains_B7024_RS14295_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539759.1"
                     /locus_tag="B7024_RS14295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_216362799.1"
                     /sec_met_domain="PF00561 (E-value: 8.2e-10, bitscore: 36.7,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2.9e-07, bitscore:
                     29.3, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVYVEDPARTGESRLSDGRLLGWAEWGPPDGVPVLCCPGAATSR
                     RLGFGAGVVEALGVRLVSVDRPGLGVSTPAPGRTFSDFAGDIRQLGALRGLGRPAMVGN
                     SQGAPFALACAAAGVVSALAVVSGADEVAAPEFVSALAADLRGLVERTARDPEGAEEFF
                     AGFSAEAMWGMVVAGSPECDLAVYQDPDFAGAYRRALADGFAQGAAGYARDTVLAMGRW
                     PFVLDEITVPVDIWYGELDTSHSPDNGASLATRMPGAHYRVVPGIGGALLWTHAEPVLK
                     SLLARAAADR"
     aSDomain        complement(7485..7919)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B7024_RS14295_Abhydrolase_1.1"
                     /evalue="7.50E-13"
                     /label="B7024_RS14295_Abhydrolase_1.1"
                     /locus_tag="B7024_RS14295"
                     /protein_end="178"
                     /protein_start="33"
                     /score="40.6"
                     /tool="antismash"
                     /translation="PVLCCPGAATSRRLGFGAGVVEALGVRLVSVDRPGLGVSTPAPGR
                     TFSDFAGDIRQLGALRGLGRPAMVGNSQGAPFALACAAAGVVSALAVVSGADEVAAPEF
                     VSALAADLRGLVERTARDPEGAEEFFAGFSAEAMWGMVVAG"
     misc_feature    complement(8968..8981)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.14"
                     /tool="antismash"
     misc_feature    complement(8990..9004)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.78"
                     /tool="antismash"
     rRNA            9099..10627
                     /db_xref="RFAM:RF00177"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /locus_tag="B7024_RS14305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /product="16S ribosomal RNA"
     gene            9099..10627
                     /locus_tag="B7024_RS14305"
     misc_feature    9142..9158
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 21.19"
                     /tool="antismash"
     misc_feature    9519..9535
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.97"
                     /tool="antismash"
     misc_feature    10353..10366
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 19.27"
                     /tool="antismash"
ORIGIN
        1 cgccgccgtc gacagccgtg acctcaccgc cctggatggc ctgttcaccg aggacgtccg
       61 cctctacagc cccgtgaagt tcacgccgtt cgagggcagg gcgatggtgc tgggcctctt
      121 cggggtgctc ctgcgcacct tcgaggactt ccgctacgtc ggagacttcg ccggcaccgc
      181 ggagaccagt gccgacggag atacggcccc ggcgacggtc ctgctcttcc gggccaccgt
      241 gaacggcaag cagatccacg gcatcgacct gctgcatttc gaccacgacg gacggatcaa
      301 ggagttcacc gtgatggtcc gcccgcagtc cgcggtgcag gcgctgggcg aagcggtact
      361 cgccggtctg gtcgccgacg gcctcctccc ggcgccggcc gatcgatagc gccggtcacc
      421 ggagaggtga tgcgggattc cgagcaggaa tccggcgtgc cgggcatcgg ggaactccgg
      481 gtgctcggtc cccgcgccgc tgtggccgtg cccgcgccgg aggccccgtt cagccggacg
      541 gtcagccgag gacgagcggg agtttcgcgg ccagttcgcc cagctcggcc ttctccgtgg
      601 cggtcggggc ccgccgcccg gtgccgacca gcagcgcgtc cttgtcggag aacgacgcgg
      661 ggaacgcggc gcgggcgatc cgctccagca tgtcgcgtgt ccgggcgagc gcctcctcgg
      721 acggtcccgc cacgtccggg aggtgcgcga ccaggtcgag gtggtgcagc gtccactcca
      781 ggacgtacgc ggagaggtag tcgcccgcgg tgaggaccac gtcgcgggtg ccgacccgca
      841 ggcccggatc ggcgagtgcg gcggcccgtc cggcggcgga gccgacgtcg tcgaggtgga
      901 acctgagcag cgacggctcc tcgtacgcgg cggccagccg gacgatcagc gcgtcgagcg
      961 ggtcgtcacc ggccggcggc gtcccggcga cgtcccagta ggtcaccgcg tcgcgggtcg
     1021 gttccgtttc ggcgggggtc accagggtga tcaggacatc ctgcgcgtcg atgatcaggt
     1081 ggcacaccag atcccgtacg agccagccgg cacagccgga cggtcgcgcg aaatcctcgt
     1141 ccggaagctg ggcgaccgcc gtgcgcaacg ctgtccacga gcgtgagaag agatccacat
     1201 cggcaagctg gtgcggtacg gaagggcgga caagcgcaga gggacggccg gttcacgtac
     1261 acggccggcg cacgtggacc gggccggcca ccctcgcgcc gaacgggtcc cgggccgcga
     1321 tggcgagctg tgatcggcgg gcctaggctg atcgaaagtc atccattgca ccgttttgtg
     1381 cggcagaagg gtgagtgacg tgagcgcttc ccgtgacgaa gcggggcggc tggagccgcg
     1441 cgtgggcccc gtccccgaag gggagccgga atctcatccg tatcaccacc ccgccgccgg
     1501 ctggggcgcg gccaagagcg tgagccggtt catggtgcgg gagggggcgc tcgccgacgg
     1561 gccgcgggcg atcctgcgga tgaaccacga gaacggcggt ttcgactgtc cgggctgcgc
     1621 atggcccgac gacaccaagg gcctgcacct cgacatctgc gagaacggga tcaagcacgt
     1681 cacctgggag atgacccgca agcgggtcgg gcgcgagttc ttcgccggcc actcggtgac
     1741 cgagctctcc gggtggagcg actacgcgct ggagaaccag ggccggctga ccgaaccgat
     1801 ggtctacgac cccggtacgg accactacct ccccatcagc tggaaggacg ccttcgcact
     1861 cgtcgggcgc accctgcgcg agctggactc cccgcaccag gcgtcgttct acacctccgg
     1921 ccggctcggc aacgaggcga ccttcctcta ccagctcatg gcgcgggaac tgggcacgaa
     1981 caacctgccc gactgctcca acatgtgcca cgaggccagc ggccgcgccc tgcaggcatc
     2041 gctgggcacg ggcaagggaa ccgtcgacct caaggactgg gaggcgaccg acgcgctgtt
     2101 catcctcggt gtcaacgccg cctccaacgc gccccggatg ctcacggccc tggcggcggc
     2161 gtaccggcgc ggcgcccagg tcgtccacat caaccccctg atcgaggcgg ccgccacccg
     2221 gacgatcatc ccgcacgacg tccgctccat ggccaccggc cacgcgaccc cgaccagcac
     2281 gctcaacctc cagccgcgca tcggcggcga cctggcgctg ctgcgcggga tggccaaggc
     2341 gatcctggag caggccacct ccgatccccg ggcgctggac cgggagttca tcgaccggca
     2401 caccaccggt ttcgaggagt accgcgcgct gtgcgaggcc acgccctggc gggagatcga
     2461 gaagcagacc gagatcagcc gccccgacat cctcaaggcc gcgcgcatct accgcgaggc
     2521 cgaccgctcc atcgtcagct ggtgcctggg catctcgcag cacgagcacg gtgtcgacac
     2581 cgtccgggag atcgtcaacc tcctgctgct gcgggggaac ctgggacggg agggggcggg
     2641 cccctccccg gtgcgcggcc acagcaacgt ccagggcaac cgcacctgcg gtatcgacca
     2701 ccgccccggc ccggagttcc tcgaccgcct cgcggaggtc tgcggtatcg agccgccccg
     2761 ggagcacggc ctggacacgg tgggcaccat ccaggcgatg caccgcggcg aggtgaaggt
     2821 gttcgtcggc atgggcggca acttcgcgct cgccgcgccc gacaccccgt acacctgcga
     2881 ggcgctgcgg aactgcgatc tcaccgtcca ggtgagcacc aagctgaacc gcagccacct
     2941 cgtccacggc cgccgggccc tcatcctgcc gtgcctcggc cgcaccgaga aggaccacca
     3001 gcgcacgggc gtccagagca cctccgtcga ggactcgatg agcatggtgc acctctccgt
     3061 cggtatgcgg cgccccgcct ccccgcacct gctctccgag ccggccatca tcgccggtat
     3121 ggcccgcgcc gccctccccg acagcgccac cccctgggag cggtacatcg aggactacga
     3181 ccgcatccgc gacaccatgg cccaagtcct ggacggcttc gaggacttca accggcgcgt
     3241 acggctgccc ctcggcttcc ggatcaagca gcccgcgcgg gaactcgtct tccgcacccc
     3301 gtccggccgc gccgagttct ccacggcccc gctgcccgac gtcgtacccg ccccggggac
     3361 gctgacgttg ggcaccctgc gctcccacga ccagtggaac accaccatct actccgacaa
     3421 cgaccgctac cggggcatca ggagactgcg cacgctcgtc ttcatgaacc aggacgatat
     3481 gcgcgaacgc ggactctcga agttcgaccc cgtcgacatc accagcaccg ccaaggacgg
     3541 cacccggcgc cacctcaacg gctatctggc cgttccccac gacatccccc gcggctgtgc
     3601 ggccggctat atgcccgaga tgaacgtcct gtgcgcgatc ggcgactaca gcacccagag
     3661 cgaccagccg atcatgaaac atgtgaaggt caccatcaac cccgccacct gagcgggggg
     3721 cggggcgctg gcggagcgcg agggcctgac gagccgcgcc ggatgcccgg acggcgggcg
     3781 cgaggggtgc cgatccggaa gcgccagatc ttctcgcgga cggcatggag gagtgggccg
     3841 gcaccgggca gggcctgtcg ggtgacctgc ggcagccatc cccgccgacc gccccgtgcg
     3901 taacccgcgc acccttcccg gactgcgcac agccccggcc gccgataccc tgaccccaac
     3961 ccgtatatcc gttttgcccc tatcgtcccc gggagtcacg tggtcaccac gcaggccgtc
     4021 gtcgtgcctc cctcgaaggc cgccgtgttc ctggtggtca cgctgaaccc cggcggtgag
     4081 gcggttgcgc gggaggcgct cgaagagctc gcggggctga tccgctcggt ggggttccgt
     4141 gcgcccgacg cggcactcac cggtgtcgtc gggatcggtg cggcggcctg gccccgcctc
     4201 ttcgacggcc cgccgccgca cgacctccac cccttccagc cgctgaccgg accccgccat
     4261 cgcgccccgg ccacaccggg cgatctcctg ttccacgtgc gcgcccggcg gatggacctc
     4321 tgcttcgagc tgtcccggct gatcgtggag cggctgggcg acgccgtgac cgtcgtcgac
     4381 gaggtgcacg gcttccggta tttcgacgag cgtgacctga tgggattcgt cgacggcagc
     4441 gagaacccgc agggcggggc ggcggccgag gccgtgttcg tgggcgacga ggacgcggac
     4501 ttccggggcg gcagctatgt gatcgtgcag aagtacgtgc acgacatgac ggcctggcag
     4561 tcgatcagcg tcgaggagca ggaacgggtc atcggacgca gcaagtcggc caatgtggag
     4621 ctgcccgacg gcgtcaaacc ggcgaactcg catgtcgcgc tcaacacgat cgtcgacgac
     4681 gagggcgccg agcggaagat cgtgcgcgag aacatgccgt tcgggaccat cggccgcggc
     4741 gagttcggca ccttcttcat cggctacgcc cgcacgcccg ccgtgaccga gcggatgctc
     4801 gacaacatgt tcctcggcga cccgccgggc aacaccgacc gcatcctcga cttctccacg
     4861 gccgtgaccg gcggtctctt cttcgcgccg agcgccggct tcctcgactc ccttcccgac
     4921 ccgcccgccg ccgctcgttc cggtgccgtc ccctcggacg gttccctcgg catcggcggc
     4981 atgaaaggag cgtgagcgcc atgacgaccc ctgccgccac gaacaacctg catcgcgaac
     5041 tcgcccctgt cacccaggcg gcctgggcgc agatcgagga agaggccggc cgcaccttcc
     5101 ggcgccatgt cgcgggccgc cgggtcgtcg acgtgcccga accggcgggc ccggccctcg
     5161 ccgccgtcgg caccggccac cgcacggaga tcgcgtcgcc cgcccccggg gtgacggcct
     5221 gcctgcggga ggcgaaaccg ctcgtcgaac tacgggtgcc gttctccgtc agcagggccg
     5281 ccgtggacga cgtcacgcgc ggctcgaacg actccgactg gcagcccgtg aaggacgcgg
     5341 cgcgcaccat ggcgttcgcc gaggaccggg cgatcttcga cggatacgcg gcggcgggca
     5401 tcgacggcct gcgggaccgg acgtcgaacc cggtgctgag cctccccgcc gagccacgcg
     5461 actaccccga cgcgatcagc cgcgccgtga catcgctgcg gctcgccggg gtcgacggcc
     5521 cgtacgcgct gcttctgggc gccgacgagt accgggcggt cagcgagacc tccgaccacg
     5581 gctacccgat cgcggcacac ctggcccgga tgctggacgg tgcaccgatc tgggccccgg
     5641 cgctcagcgg cgccttcgtc ctgtccaccc gcggcggcga cttcgaactc cgcctcggcc
     5701 aggacgtggc catcggctac acctcccacg acgccagcag catcaccctg tacttccagg
     5761 agacgctgac cttcctgacg gacacggacg aggctgtcgt cgtgctgaag cactgagcaa
     5821 acgccatgcg ggggatgccg cccgggtgcg gggtggctcc gccgccggat cgcccgtgac
     5881 cgtcacccgg cccggcgagc tgccatccat cccggccacg ctctcctcta tatacgcgta
     5941 tatccgtgcg ggccgcctct cccgcccgct gcgacgggcc gtggacgcgg acctcgccgc
     6001 cgcgggtggc gagcatcagg gcgatcccgc gtaccggagt gcccgcgccg acgacggcga
     6061 ccggccggtt cccgacgtcc gcgggagagg ggaaagccgt tcccggtctg ggggaggagg
     6121 ccgcaccgac ccggcaccgc cgcgccccgc tcagcagctt cggccgggac gtgtcgaagc
     6181 ggccaccacc gctactccgg tggcgggacc gctacgcgcg cggcccgtcg tccgggggag
     6241 cgccgccggg cccgagggac aggacgacgg cggtggtgct gccgtcgggc gtctcgtagg
     6301 ccaccgtgtc accggtgcgg cgcccgagca gcgcacgccc gagcgggctg tcggcggtca
     6361 ccagggtccg gttcggttcc tccacgatct cgccgatccg gacggtcgcc tccgcaccgt
     6421 ccgcgaagcg cacggtgacg gtactgccca cccccaccat gtccgtgggc ggcggacccg
     6481 cgacggacgc ctcgcggatc cgcccctcga tctcggcgat gcgtccgtcc agccgcgcga
     6541 cctccgaggc gcgctgcaac gcgtcggcct cgtcggcccg gtcacccacc gggtcctcgc
     6601 tccgcaaggt cgcggccacc ggcccccgtt cggcgcgcag ctcggccagc tcctgccgga
     6661 gcgctcgaag ggcgtcaacg ctgatgggct cgggaccctc ggccatgcct gctcccgtgc
     6721 tgccggtgga acgatcggga catctatgta cgggacacct atgtacggga catctacgta
     6781 cgagatattt acgtacaagg catctcaatt agcgcatcac ccaccgggcc cggcaaatca
     6841 tcgaagccgg cacatcagca ggccggggga gcgcaccaac aggccgatgg cacgagccga
     6901 agcagggcgg cgtccctcac atcccctgcc cgtgcccatg gcgtgcgcat agccatgctc
     6961 atgtccatac atgcacgggc cgggtcatcc gcagcaatgc cgaccgggct ccggcgatgt
     7021 tcccgccccc gtaccgcccc cggcccggcc ccgggaaggt caccgaccac ccatccgtca
     7081 cagcacacgc cacccgtccc ggcacgcccg ggaccgcgcc gtccaccggt cggcggcgcc
     7141 ctctaacgat cggcggcggc tctcgcaaga agcgacttga ggaccggctc ggcatgtgtc
     7201 cacagcagcg cgccgccgat ccccggtacg acgcggtagt gggcgccggg catacgggtg
     7261 gcgagcgatg ccccgttgtc gggtgagtgg ctggtgtcca gctcgccgta ccagatgtcg
     7321 accgggacgg tgatctcgtc gaggacgaac ggccaccgcc ccatggccag gaccgtatcg
     7381 cgggcgtagc cggccgcacc ctgcgcgaag ccgtcggcca gggcccggcg gtaggcgccc
     7441 gcgaaatcag gatcctgata caccgcgagg tcacactccg ggctcccggc cacgaccatg
     7501 ccccacatcg cttccgcgct gaaccccgcg aagaactcct cggcgccttc cgggtcgcgc
     7561 gcggtccgct cgacgaggcc acgcagatcc gccgccagag cggaaacgaa ctccggcgcg
     7621 gcgacctcgt ccgcgccgga gacgacagcc agcgcggaga cgacgcccgc cgcggcgcag
     7681 gcgagcgcga acggcgcacc ttgcgagttc ccgaccatgg ccgggcgccc cagcccccgc
     7741 agcgcaccga gctgacgaat atcaccggcg aagtcggaga acgtccggcc gggtgcgggc
     7801 gtggagacac cgagccccgg gcggtccacg gagacgagac gcacgccgag cgcctcaacc
     7861 acgcccgcgc cgaagccgag ccgccgactc gtggctgccc ccggacagca cagcaccggc
     7921 accccgtccg gcggccccca ctcggcccag ccgagcaacc gtccgtcgga cagccggctc
     7981 tcaccggtcc gggcaggatc ttcgacgtac acgttcatga ccgacatcat gaccacggac
     8041 ggaacacttc gcaccggact ttcaccgcgg ctccgcaccg ggccgacgcc accggacccg
     8101 gcaccgctgc ggcccccgca cctgcgggtt gcccccaagg caccgacggc accgtccacg
     8161 gactctcccc gctgaccgga gaaacgcact ctccccactg accggagaag gggccacacg
     8221 cgaaggccgg ccggtccagc ggtgcgggag cggcccgccc cggcccagat gccgtcgggg
     8281 gtgtgttgat cccgggacag atcaccggaa tacgctcgcc tccttgtcgg caccccgtga
     8341 tcaggcggtc cggcgcccca gcggtcccgg gcagcgcgaa ccaccgcggg cgtgccccca
     8401 agtcgtgccg cacccatacg atcgtgtaag ttctacgagt tgccgcggaa ccgacaggtc
     8461 ccacggcagc gatcccgccg cttcagcggc acaccactac tgcacggtcc ctcaccggga
     8521 agaatttcgg catgccggaa ttcggaccgg gactcgatta tgagtcaccg aggaattccg
     8581 ctaaagtagt gatcacgccg aaaggcgaaa cccctccgac gggtaatcgg aaccgaattc
     8641 gaaccggaaa cggaacgggc cgggtctgat agagtcggaa aggccggaaa gcgaaagcgg
     8701 gaaggccgac cctgatttca cagggggctg gatacggaaa cggatctggt acggtgggaa
     8761 agcgaagaag ccggaaggcg gaaacgtaga ccggcggaaa tcgggtccgg aaggatctga
     8821 tagagtcgga aacgcaagat cgaagggaag cgcccggaga gcctgatgaa acaggcgcaa
     8881 aggaagcgtc cgttccttga gaactcaaca gtgtgccaaa agtcaacgcc agatatgttg
     8941 ataccccgtc cacttcggtg gatgaggttc ctttgaaaaa gtcctccgac gccacatgtg
     9001 gtgtgggagg cgacactagc gaggacgctg tgaactgctg gacttattcc gtccggcggt
     9061 tccgctctcg tggtgtttgc cccggtatcg gggagacatt cacggagagt ttgatcctgg
     9121 ctcaggacga acgctggcgg cgtgcttaac acatgcaagt cgaacgatga accggtttcg
     9181 gccggggatt agtggcgaac gggtgagtaa cacgtgggca atctgccctt cactctggga
     9241 caagccctgg aaacggggtc taataccgga tatgacgcac gaccgcatgg tctgtgtgtg
     9301 gaaagctccg gcggtgaagg atgagcccgc ggcctatcag cttgttggtg gggtgatggc
     9361 ctaccaaggc gacgacgggt agccggcctg agagggcgac cggccacact gggactgaga
     9421 cacggcccag actcctacgg gaggcagcag tggggaatat tgcacaatgg gcgaaagcct
     9481 gatgcagcga cgccgcgtga gggatgacgg ccttcgggtt gtaaacctct ttcagcaggg
     9541 aagaagcgag agtgacggta cctgcagaag aagcgccggc taactacgtg ccagcagccg
     9601 cggtaatacg tagggcgcaa gcgttgtccg gaattattgg gcgtaaagag ctcgtaggcg
     9661 gcttgtcgcg tcggatgtga aagcccgggg cttaaccccg ggtctgcatt cgatacgggc
     9721 aggctagagt tcggtagggg agatcggaat tcctggtgta gcggtgaaat gcgcagatat
     9781 caggaggaac accggtggcg aaggcggatc tctgggccga tactgacgct gaggagcgaa
     9841 agcgtgggga gcgaacagga ttagataccc tggtagtcca cgccgtaaac gttgggaact
     9901 aggtgtgggc gacattccac gtcgtccgtg ccgcagctaa cgcattaagt tccccgcctg
     9961 gggagtacgg ccgcaaggct aaaactcaaa ggaattgacg ggggcccgca caagcagcgg
    10021 agcatgtggc ttaattcgac gcaacgcgaa gaaccttacc aaggcttgac atacaccgga
    10081 aacggccaga gatggtcgcc cccttgtggt cggtgtacag gtggtgcatg gctgtcgtca
    10141 gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaacccttg ttctgtgttg
    10201 ccagcatgcc tttcggggtg atggggactc acaggagact gccggggtca actcggagga
    10261 aggtggggac gacgtcaagt catcatgccc cttatgtctt gggctgcaca cgtgctacaa
    10321 tggccggtac aatgagctgc gataccgtga ggtggagcga atctcaaaaa gccggtctca
    10381 gttcggattg gggtctgcaa ctcgacccca tgaagtcgga gttgctagta atcgcagatc
    10441 agcattgctg cggtgaatac gttcccgggc cttgtacaca ccgcccgtca cgtcacgaaa
    10501 gtcggtaaca cccgaagccg gtggcccaac cccttgtggg agggaatcgt cgaaggtggg
    10561 actggcgatt gggacgaagt cgtaacaagg tagccgtacc ggaaggtgcg gctggatcac
    10621 ctcctttcta aggagcattt cttaccgagc ttgtcttggt cagaggccag tacatcggcg
    10681 aatgtccggt gctggttgct catgggtgga acgttgacta ttcggcacat cgggttggtt
    10741 gtttccagta ctgcttcggc gtggaacggg atgacggatc gggtgtgtcg ggcacgctgt
    10801 tgggtatctg agggta
//
