LOCUS       NZ_NDXL01000001        21513 bp    DNA     linear   CON 27-APR-2024
DEFINITION  Streptomyces kasugaensis strain BCRC 12349 scaffold1, whole genome
            shotgun sequence.
ACCESSION   NZ_NDXL01000001
VERSION     NZ_NDXL01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces kasugaensis
  ORGANISM  Streptomyces kasugaensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Comparative genome analysis for kasugamycin overproduction
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Comparative genome analysis for kasugamycin overproduction
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030,
            China
REFERENCE   4  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. May-2015
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 188.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002261115.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 02:42:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,951
            CDSs (total)                      :: 7,863
            Genes (coding)                    :: 7,657
            CDSs (with protein)               :: 7,657
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 4, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 4, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 87 of 206
            Pseudo Genes (incomplete)         :: 170 of 206
            Pseudo Genes (internal stop)      :: 23 of 206
            Pseudo Genes (multiple problems)  :: 65 of 206
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:37:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2355201
            Orig. end                         :: 2376714
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NDXL01000001.1.
            Bacteria and source DNA available from BCRC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21513
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="triceptide"
                     /region_number="16"
                     /rules="((cds(PF04055 and (TIGR04085 or SPASM)) and
                     triceptide_precursor) or triceptide_rSAM)"
                     /tool="antismash"
     cand_cluster    1..21513
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="((cds(PF04055 and (TIGR04085 or SPASM))
                     and triceptide_precursor) or triceptide_rSAM)"
                     /kind="single"
                     /product="triceptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21513
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11513](+)"
                     /cutoff="5000"
                     /detection_rule="((cds(PF04055 and (TIGR04085 or SPASM))
                     and triceptide_precursor) or triceptide_rSAM)"
                     /neighbourhood="10000"
                     /product="triceptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11513
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="((cds(PF04055 and (TIGR04085 or SPASM))
                     and triceptide_precursor) or triceptide_rSAM)"
                     /neighbourhood="10000"
                     /product="triceptide"
                     /protocluster_number="1"
     misc_feature    complement(624..639)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.57"
                     /tool="antismash"
     gene            complement(845..1834)
                     /locus_tag="B7024_RS10230"
     CDS             complement(845..1834)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986910.1"
                     /locus_tag="B7024_RS10230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="WP_094791740.1"
                     /transl_table=11
                     /translation="MSDRTEWSISAPQKLEFEAPVTALNVRVIGGTVNVVGTSDGGPPR
                     LEISELTGPPLLVSCRDGVLSIGYEDVTWPGILGQFDPKEWGRGLFDPKAWRHGLKGWN
                     PTGWHRSAVISVTVPAATRVEVGAATASAVISGISGRTELRGVSGDGTLAGLTGPVRAD
                     TVSGRIEAQAVTGELRFHSVSGDLTVIEGAGGSVRADSVSGDMVLDLDLPPAAPDGTAA
                     ARTDISLTTVSGEVAIRLPDPADTRVTANTTSGTVSNAFDDLRVSGQWGAKRITGSLGA
                     GTGTLKVTTVSGGLALLRRPAAEEPHGAPGGGPDGAAPTGTPTEKKVR"
     misc_feature    complement(1810..1825)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.88"
                     /tool="antismash"
     misc_feature    complement(1874..1892)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.33"
                     /tool="antismash"
     misc_feature    complement(1882..1895)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 19.02"
                     /tool="antismash"
     gene            complement(2064..2243)
                     /locus_tag="B7024_RS10235"
     CDS             complement(2064..2243)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889383.1"
                     /locus_tag="B7024_RS10235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6104 family protein"
                     /protein_id="WP_094791741.1"
                     /transl_table=11
                     /translation="MYFTDRGIEELENRRGEEEVTLGWVADQLRTFVDLNPDFEVPVER
                     LATWLARLDDEDED"
     misc_feature    complement(2233..2248)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.19"
                     /tool="antismash"
     gene            2395..2814
                     /locus_tag="B7024_RS10240"
     CDS             2395..2814
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018087463.1"
                     /locus_tag="B7024_RS10240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CU044_2847 family protein"
                     /protein_id="WP_052856491.1"
                     /transl_table=11
                     /translation="MQDRAQRIALPDGTEVWARVSRLDAAGLDDPVGPDGEYADFEDVG
                     TWEALGARVEGLREVIGGVAASVRQATERVAPHETSVTFGVEVSAKPGKAVALLADGEA
                     KANLSVTLTWRREDGNASGGEQPAVGRREPADPGR"
     gene            2814..5012
                     /locus_tag="B7024_RS10245"
     CDS             2814..5012
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006603152.1"
                     /locus_tag="B7024_RS10245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="trypsin-like peptidase domain-containing protein"
                     /protein_id="WP_094791742.1"
                     /transl_table=11
                     /translation="MATSEERAHGRGDRARYGQLLAHAGRTTVALRAAPDAPDPRLWGS
                     GVLIAPGWVLTCAHVLAASDGRRRDLGPDGVFGVAFGGRLAAARLEYDLSRPDPAAGPG
                     TARADLALVRLLDPDTEHPCAWLSDQPATLLEDAYIFRGHDRPGTDRPSRTDAAHERVV
                     RDGGGVRAAGDAAEADGAGEAGGAPGAPGGAADPFTPFIAVRFGARDARGLQFGSDVRV
                     TPGASGGPLLDCDRGEVVGIVKGRHQQDHVGLAVPVTALRGLGPEHLVAGATGLGPDPY
                     HALMGLHDRWHWSGQDLGRPGGPTWFDAQHAIMAGRGRLWGVQERLQALDLLARLPAPR
                     DPLVVEAAVGEALDRGEGPGAWALRTWRDGHGALYQGSDPYTELCAFVHYLRIVAQRVA
                     DEAREAPGEAAAEVREHAAHLAEFVQAKAVVLQPQDRRRIGPVRRRPRSVLVEFEPLFY
                     DDGGGELFNWSVSEGYGRGQWLRVDVQESDGGVPFEQARAQVLRRLGARLLRADGDAGP
                     GARVRLEVAVPEGYWHTPADRWEVAASTRRTARARPVGPGRAVILRDQGRREEIDPAWL
                     RRWQRVAVAPALHALRVPPPRRDGAGAAAGSADGVWRLLEEADDGAVPALCHTVADGTG
                     RDAAGAALDTGFPAGLWSAAGHGERSCDASCEEFHRGVRELLQGSGSLARLPELVRELR
                     AKAAEEGAHWARDLVLLYDDPEDPIPQLFTARPQVSPR"
     gene            5430..6509
                     /locus_tag="B7024_RS10250"
     CDS             5430..6509
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006603151.1"
                     /locus_tag="B7024_RS10250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MoxR family ATPase"
                     /protein_id="WP_094791743.1"
                     /transl_table=11
                     /translation="MSDWLIYRGAGAPHDGIEQLPPPPPWRDFDGGPDIPPPTALDPAS
                     ERRLGVLRREASHHRPGETERELVNAALYLRRPLLVTGAPGSGKSTLAHSVAYELGLGR
                     VLSWPVVSRSTLRDGLYAYDAIGRLQDLQIARATPPRPAGQDAPEGAAPPAPDPDHTAE
                     PGGGIGRYIRLGPLGTALLPAARPRVLLVDELDKSDIDLPNDLLNVLEEGEFRIPELER
                     LAGSAPEVRVLSDDGAPVTVRDGRVRCHAFPFIVLTSNGERDFPAPLLRRCIHLHIPAP
                     DKERLAAMVRAHFGEGAAERHASVIDRFLDREPGDVRAVDQLFNAIHLTQQAGWTDQDE
                     EETRRRLTAELMRPLDRTR"
     misc_feature    5470..5485
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 20.97"
                     /tool="antismash"
     gene            6515..9895
                     /locus_tag="B7024_RS10255"
     CDS             6515..9895
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006603150.1"
                     /locus_tag="B7024_RS10255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SAV_2336 N-terminal domain-related protein"
                     /protein_id="WP_094791744.1"
                     /transl_table=11
                     /translation="MAVGESGPGPLDQLVARLTAAGLPADARGMADALWLAQWITPGDQ
                     QTARDATGEAGSTTAGCSDPATFTVGPAGPVEPGAAAAPEPGGPYAPRDGARRTEAELR
                     PAREARPPDPDRHRLGEVAVPIAAAFPGLLPLQRALRPVQRYHPPVAPVRRELDEPATA
                     ELSAHAETVIPVLRGVHRREAGLRLLMDGSSSMAVWEQMLHDLRQVCEQVGAFRDVTVH
                     YLHPHGPDVGVAAAPGPGRPLRPAGQLHDPTGHHLTLVVSDCAGPLWQDGRLQRLLHRW
                     AADAPVAVVQPLPQRMWARTRLPAVAGTLVRHQGPYQALGFRPARRRRAPAAPGPAPDA
                     GRGRAIPVLSATPAALGAWARLAAADAGLSLRGAACVVRADHGAEPGGRPAEAEPSAPA
                     RRVREFDRSASPDARLLAVHLSAVPLALPVIQLVQRAMLPHTGPAELAEVLLGGLVTQL
                     PAADRSTGEADGASGPWYDFVPGVRDELLGRLSAGEAALVLKHCSLYVERTFGRGARNF
                     PAVAVAMLSGSGREPESGRRPVPEPFARVSEHVLRRFEPALRPPEQPPYPGDGPAAEGA
                     ALLERYEQGSAVRDLIEAVRLLRAAAHRLPPAGTDLARALLHAWAKWRQPDALAEAERA
                     VRAAERATAPGPADDQEVGTAGEEAGAAEAAGREAERTRALIVLGRVRYARARERAGAG
                     APEEERTALADAARQLHAACGLMSLLDPRFLESTVLRTEVLRRVAALPPATGAPAGPGA
                     PEGAGPPDPLDEAERSLTTVLDRWPSGEQVPGEVYAARGGVLLDQARRAGRVRPDGPRP
                     AEALAVRAADDLDTATLLLRRRGSAADRVVCETLLDLAAARSLTGGSALPSVLPTLERA
                     RAMARTLAEPALEADSLRRIAAAQRAVHTRTGAPGPLDEAIAALAAAQRLTVPDSPEHS
                     ALLAERGAALLLRARLEPDPEPGRRLANDAVHVLREALARVAPQDPELGGHRLAFGRAL
                     RLRHERQPSLADLHEADWILELAARGSGVEPLVAAEAWLEVGDVQLLLDRRFDSRERHD
                     RAAACYRRAATHAHRGGSPLLAARAHHRRGEVLEETAGPQPALHAYRESWEQWQHAAEE
                     SGPEAQRTRARMRALESPA"
     gene            10001..10219
                     /locus_tag="B7024_RS10260"
     CDS             10001..10219
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     triceptide: triceptide_precursor"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006603149.1"
                     /locus_tag="B7024_RS10260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YxD-tail cyclophane-containing RiPP peptide"
                     /protein_id="WP_052856487.1"
                     /sec_met_domain="triceptide_precursor (E-value: 1.0,
                     bitscore: 10.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATIPRTEPAGAAPSGPSAEPLPDFGGVDVAGLAARTDHAVLGEV
                     AALLLRNWPSADDAVAYYEDGPEKLLH"
     gene            10371..11513
                     /locus_tag="B7024_RS10265"
     CDS             10371..11513
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     triceptide: triceptide_rSAM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) SAM_SPASM_FxsB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018087459.1"
                     /locus_tag="B7024_RS10265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FxsB family cyclophane-forming radical SAM/SPASM
                     peptide maturase"
                     /protein_id="WP_172450538.1"
                     /sec_met_domain="PTHR43273 (E-value: 2e-80, bitscore:
                     268.6, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="SAM_SPASM_FxsB (E-value: 1.9e-127,
                     bitscore: 423.2, seeds: 10, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 2.3e-12, bitscore: 45.6,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="triceptide_rSAM (E-value: 1.0, bitscore:
                     1.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVAVETGRTTVPFRQFVLKMHSRCNLACTYCYIYAGPDRSWRARP
                     RLVPTATLRQSALRIAEHARAHRLREIRIDLHGGEPLLTGPGPLLDQAAALRAALPAGC
                     TAEFGVQTNGTLLTPELIDTLGAAGFRLGLSLDGGTPGLNHRRVDHAGRPSWPAVSRAV
                     RMLADRPGVFAGILSTIDITTDPAEVYGSLRALGPPVLDFLLPHANWSSPPPAPLGPAR
                     RTPYGDWLCTVFDLWWDGGPGPRVRLFTEIIGLLLGRPSSSEAVGLSPVVALVVDTDGA
                     IEQVDSLKSTYEGAAATGLDVFHHSFDEALAHPGMAARRRGPDALGAQCRSCALMRVCG
                     GGNYAHRYRAGPSGFANPSVYCADLERLIRHIAARLAAAM"
     gene            11648..14722
                     /gene="fxsT"
                     /locus_tag="B7024_RS10270"
     CDS             11648..14722
                     /codon_start=1
                     /gene="fxsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006603147.1"
                     /locus_tag="B7024_RS10270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FxSxx-COOH system tetratricopeptide repeat
                     protein"
                     /protein_id="WP_094791745.1"
                     /transl_table=11
                     /translation="MADQSTFSDSSGSSGTSGSSGSPGPAGTSGPARSHEHITISYAGF
                     NRPWAAWISHQLEQLGHGTTLLRWDPVPETPLADELSGLLAAEGRLLMVLDDWYFRLGP
                     KSHEEWTDALQEVVTPHADRFAAVSVATQKLPATAAVLRPADLRDLDAVEANRRVLQRL
                     GVTSARRPRGEPSPGAPRFPNDPPEVWNVPRRNNRFTGRDHVLEELHDKLAGGGLGAGP
                     PLETRIALYGISGVGKSQIAAEYAHRFGNDYDVVWWISATNRGAAREQLAELANRLGLP
                     VGHELGDRIRAVHEALRVGRPYRRWLLVFDSADDMEQIEDLVPDGRGHVLITTLTRDWS
                     GSGSAQEIEVRPFNRMESVAYARRRAPRLTPLEADLLAEAVQDLPLLLAQTAAWLDANP
                     MSPKEYIELIRRGEPSLVGIRISSDYPMAFQTSWAITLNTLRERSPAAVELLKLFAFFT
                     PDAIPVPMLAKARRGDLPEHLADLAAEPISWNTALRRLTESTAVRLDYQLSEESDPAVE
                     TAQMHRLYHRFLRSDMTEDERDQMSATACRVLVTADPQNPSDTRYWERYAELIPHLEPA
                     GVLDSPEPAVQELLLNCIEYLRVRGENRMGLRLCEQFLARWSTRMEPTRRTMLVLTHQH
                     ANMLRRMGRYREAEAVGSAIVDQLAAERTPADSDLLRAQDGLAGTLMALGSYEEAYTLF
                     DTVWRTYTEVLGPEAPRTLSSRHNLGSALALLGRYEDALGTFRDVLAFRERELRARHHL
                     TLNAGASYSRMLRLLGRYRDATSSQELNARLHRQVMGSHTPQTLRAEHDLALCLRRSGE
                     LGRADALMADLVERSRRVQGPRHPETLMVRADYATFLREHGDLGHARDLAEEIAERYRA
                     LAGADHPYTIGTLGNTGLARGAFGELTEALDIAERALGDMTRIMGPDHPWTLGCALNTA
                     AARNRVEDEDGAVELSHDTLERAKAALGGTHPLTLSAKTALAEDLRTLRRGQEAAKLEQ
                     EAVQQLAETLGAEHPHTLSARRRRRPYWDFEPQPV"
     misc_feature    11664..11673
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.82"
                     /tool="antismash"
     gene            complement(14831..18610)
                     /locus_tag="B7024_RS10275"
     CDS             complement(14831..18610)
                     /EC_number="4.1.1.71"
                     /GO_function="GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018102698.1"
                     /locus_tag="B7024_RS10275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="WP_094791746.1"
                     /transl_table=11
                     /translation="MSLQSPNSSSISTDQEGQGKNPAAAFGPNEWLVDEIYQQYLQDPN
                     SVDRAWWDFFADYKPGGAAAPSQPTEAIAPAPAAPATPAVPAAPTTPAAPAATSAPAGS
                     SAQQGAAPAAPAPAKPAPSAPAPAAAKPAAAKPAGPELVTLRGPSAAVAKNMNASLELP
                     TATSVRAVPVKLLFDNRIVINNHLKRARGGKVSFTHLIGYAMVQALKAMPSMNYSFAQK
                     DGKPTLVKPEHVNLGLAIDLVKPNGDRQLVVAAIKKAETLTFFEFWQAYEDIVRRARDN
                     KLTMDDFTGVTASLTNPGGIGTVHSVPRLMPGQGLILGVGAMDYPAEFQGTSQDTLNKL
                     GISKVMTLTSTYDHRVIQGAASGEFLRVMSQLLLGENDFFDDIFKSLRIPYEPVRWLRD
                     IDVSHDDDVTKAARVFDLIHSYRVRGHVMADTDPLEYHQRKHPDLDIIEHGLTLWDLER
                     EFAVGGFAGKTMMKLRDILGVLRDSYCRTTGIEFMHIQDPKQRKWIQDRVERSHKSPER
                     EEQLRILRRLNSAEAFETFLQTKYVGQKRFSLEGGESVIPLLDAVIDSAAESRLDEVVI
                     GMAHRGRLNVLANIVGKSYAQIFREFEGNLDPKSMHGSGDVKYHLGAEGTFTGLDGEQI
                     TVSLAANPSHLEAVDPIVEGIARAKQDLINKGGTDFTVLPVQLHGDAAFAGQGVVAETL
                     NMSQLRGYRTGGTVHIVINNQVGFTAPPESARSSMYATDVARMIEAPIFHVNGDDPEAV
                     VRVARLAFEFRQAFNKDVVIDLICYRRRGHNESDNPAFTQPLMYDLIDKKRSVRKLYTE
                     SLIGRGDITLEEAEQALQDFQGQLEKVFTEVRDATTAPMTPEVPQPQAEFPVAVDTAIS
                     QEVVKRIASSQVNIPDRVTVHPRLLPQLQRRAAQVEDGTIDWGMGETLAIGSLLMEGTP
                     VRLAGQDSRRGTFGQRHSVLIDRETGEDYTPLLYLSEDQARFNVYDSLLSEYAAMGFEY
                     GYSLARPESLVMWEAQFGDFVNGAQTVVDEFISSAEQKWGQTSGVTLLLPHGYEGQGPD
                     HSSARIERFLQLCAQNNMTVAMPTLPSNYFHLLRWQVHNPHHKPLVVFTPKSMLRLKAA
                     ASKTEEFTTGGFRPVIGDTTVDAAAVRKVVFCAGKVYYDLEAEREKRGAHDTAIVRIER
                     LYPLPGKELQAEIAKFPNAEKYLWAQEEPANQGAWPFLGLNLIDHLDLAVGADVPHGER
                     LRRISRPHGSSPAVGSAKRHQAEQAQLIAEVFDA"
     gene            complement(18848..19963)
                     /locus_tag="B7024_RS10280"
     CDS             complement(18848..19963)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570287.1"
                     /locus_tag="B7024_RS10280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /protein_id="WP_094791747.1"
                     /transl_table=11
                     /translation="MTRRWRAPARGLWARVWDGLRPLDPYRSVKAALGALVIVSVIITT
                     LLVFVAMHSATELRVITIFSIIASLLITQFVANSLTAPLGEMTDVTQSMAHGDYTRRVH
                     SARRDEFGDLADAFNRMAADLEAVDTHRKELVANVSHELRTPIAALRAVLENVVDGVSQ
                     PDPETMRTALKQTERLGRLVEHLLDLSRLDNGVVPLHTTRFEVWPYLSGVLKEANMSRG
                     AATLAGSGTHTRTDVHLHLDVSPPELTAHADAERLHQVVANLIDNAIKHSPRHGRVTVH
                     ARRGEGPGSLELDVQDEGPGIPESERYRVFERFNRGGPAASGPGSDGGTGLGLAIARWA
                     VDLHGGRIGVAESPRGCRIRVTLPGLEPARS"
     gene            complement(19960..20700)
                     /locus_tag="B7024_RS10285"
     CDS             complement(19960..20700)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536131.1"
                     /locus_tag="B7024_RS10285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_052856484.1"
                     /transl_table=11
                     /translation="MDQTQTSQGGTAAATPGAQRRVLVVEDDPTIVDAIAARLRAEGFQ
                     VQTATDGPAAVDTAEAWQPDLLVLDVMLPGFDGLEVCRRVQAQRPVPVMMLTARDDETD
                     MLVGLGVGADDYMTKPFSMRELAARVHVLLRRVERAALAAHTPRSGILRLGELEIDHAQ
                     RRVRVRGTDVHLTPTEFDLLVCLANTPRAVLSREQLLAEVWDWADASGTRTVDSHIKAL
                     RRKIGAERIRTVHGVGYALETPAA"
     gene            complement(20778..21509)
                     /locus_tag="B7024_RS10290"
     CDS             complement(20778..21509)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570289.1"
                     /locus_tag="B7024_RS10290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="spermidine synthase"
                     /protein_id="WP_052856483.1"
                     /transl_table=11
                     /translation="MAPALPQHSTAGLPEAPRTLDRREGPYGEVALRQRDGGPGAPPVY
                     EIIANGCFLMDTSDGRSERLLIDAALAELPAARPRPAVLIGGLGVGFSLARAAAEPRWG
                     RIAVVEREAAVIDWHRTGPLSAVSAAALADPRTEILATDLVAYLRTGADGDTYDALCLD
                     IDNGPDWTVTDGNGSLYSPAGLAACRDRLAPGGVLAVWSAQPSPGFEQALRNAGFSGVR
                     TKEIPVVRGVPDVVHLARKGA"
ORIGIN
        1 tggccggtcc ggtcccgggc gccgccgtgg tccgcgccgg tctgcccgcc cgtgaagcgc
       61 ccgacctcgc gggacacctc ggacagcgcc tcgcgcaccg cccccgccca gtcgccggac
      121 cggacatgct cctgggcctg ctcctggacg cgcttgatga cctgctggac ctcctcgcgc
      181 gcgaacacct gggcgtcccg ggcctgctta cgggcccgct gggcgtcctg ccgggcccgc
      241 cggctctcct ccttcgcccg ccgcgcctgc tccttccacc cctccttggc gcgccggaac
      301 tcctccttcg cctgccgcca ggactccttg tcggcgaacg cgccgtcgaa gtagtccgag
      361 gcttccggga aggggcggcc ggtgccgccg tcctgcgggg cgccgctccg cccgcccttg
      421 gcgccgcccc cgaaggcgct cgcggcggac tccttgcggg cctgctgcgc ggcctcctgc
      481 atctcgcggc gcaggtcccg cgcggagccg cggacgtcct cccggatgtc ggaggccagc
      541 gcggccaccg actcccggat ctccagctcc agatcggcca gctcgccgcc ccggtcggcg
      601 agttcggccc ggccggcgtc ggtgatggag tagaccttgc ggccgccctc ggtggcgtgc
      661 gtgaccaggc cctcggcctc cagcttggcc agccgcgggt agaccgtgcc ggccgagggg
      721 gcgtacaggc cctggaagcg ctcctccagg aggcggatga tctcgtagcc gtgccggggg
      781 gcttcgtcca gcagcttgag gagatagagc cggaggcggc cgtgggcgaa gacggggggc
      841 atgctcagcg caccttcttc tctgtggggg taccggtggg ggcggcgccg tccggtccgc
      901 cgccgggggc gccgtgcggc tcctcggcgg cggggcggcg gagcagggcc agcccgccgg
      961 agacggtggt gaccttcagt gtgccggtgc ccgcgccgag cgagccggtg atccgcttgg
     1021 cgccccactg gccgctgacc cgcagatcgt cgaaggcgtt ggagaccgtg ccgctcgtgg
     1081 tgttggccgt cacccgggtg tcggccgggt cgggcagccg gatggcgacc tcaccggaga
     1141 ccgtcgtcag gctgatgtcc gtccgggcgg cggcggtccc gtccggggcg gcgggcggca
     1201 ggtccagatc caggaccatg tcgccgctga cggagtcggc gcgcaccgaa ccgcccgcgc
     1261 cctcgatcac cgtcagatcc cccgagacgg agtggaaccg cagctcgccc gtgacggcct
     1321 gggcctcgat ccggccgctg acggtgtcgg cccgcaccgg gccggtcagc cccgcgaggg
     1381 tgccgtcacc ggagaccccg cgcagctccg tacgccccga gataccggag atcaccgcgc
     1441 tcgcggtggc cgcgccgacc tcgacgcggg tggcggccgg gacggtcacc gagatcaccg
     1501 cgctgcggtg ccaccccgtg gggttccagc ccttgaggcc gtgtcgccac gccttggggt
     1561 cgaagaggcc gcggccccat tccttggggt cgaactggcc caggatgccg ggccaggtga
     1621 cgtcctcgta cccgatggac aggacgccgt cccggcacga gacgagcagt ggcgggccgg
     1681 tcagctcgct gatctccagg cggggcgggc cgccgtccga ggtgccgacg acattgacgg
     1741 tgccgccgat cacgcgcacg ttcagcgccg tgacgggcgc ttcgaattcc agcttctgtg
     1801 gtgcggagat cgaccactcg gtccggtctg acatttctgg cctccccggg caggcggaca
     1861 acgcaacata tcgcgtcttc tttagacacg atatatcgcg ggttcgggaa gtcaagcgcg
     1921 ccccgtgccg tgacgggcgc cccaccggac ccgtgccgcc cgcccgtccc cgtccgcgca
     1981 ccggcggccc gcccggtacc gggccgccat gcctcctcgg ccggctgccg tcctcagccc
     2041 tcgtcagtcc tcgtcagcct gcgtcagtcc tcgtcctcgt cgtcgagccg ggccagccag
     2101 gtagccagcc gctccaccgg cacctcgaaa tccgggttca ggtcgacgaa cgtacgcagc
     2161 tgatcggcca cccagccgag ggtgacctcc tcctcgccac gccggttctc cagctcctcg
     2221 ataccgcggt cagtgaaata cacgtgcgct ccaccagggg tcatgggaca ttccggccag
     2281 gataggcagg cccgcacggc tctcgcgccg cgggcacccg ggagctaacc tgagcgcctg
     2341 tcaacggtcc tgcggcgatg gggaattgcc gccaacgggg agcgggaggc cgtgatgcaa
     2401 gatcgcgcgc agcgcatcgc actgcccgac ggtacggagg tctgggcccg ggtctccaga
     2461 ctggacgccg cggggctgga cgatccggtc gggccggacg gcgagtacgc cgacttcgag
     2521 gacgtcggca cctgggaggc gctcggcgcc cgggtcgagg ggctccgcga ggtgatcggc
     2581 ggggtcgccg ccagcgtccg gcaggccacc gagcgggtgg caccgcacga gaccagcgtg
     2641 accttcggcg tggaggtctc ggccaaaccg ggcaaggccg tcgccctcct cgcggacggc
     2701 gaggcgaagg cgaacctctc ggtgacgctc acctggcggc gcgaggacgg gaacgcgtcc
     2761 ggtggcgagc agcccgccgt cggccgccgg gagccggccg acccgggccg ctgatggcga
     2821 cctcggagga gcgggcccac ggccgcggcg accgggcgcg gtacggacag ctcctcgcac
     2881 acgccggacg caccaccgtc gccctgcgcg ccgcaccgga cgcaccggac ccccggctgt
     2941 ggggcagcgg tgtgctgatc gcccccggct gggtgctgac ctgcgcccat gtgctcgccg
     3001 cgagcgacgg gcggcggcgc gacctcggcc ccgacggggt gttcggcgtc gcgttcggcg
     3061 ggcggctggc ggccgcccgg ctggagtacg acctgagccg gcccgacccg gcggccggac
     3121 cgggcaccgc ccgcgccgat ctcgccctgg tccggctgct cgacccggac accgagcacc
     3181 cctgcgcctg gctcagcgac cagccggcga cgctcctgga ggacgcgtac atcttccggg
     3241 gccacgaccg gcccggtacg gaccgcccgt cgcgtacgga cgccgcgcac gaacgcgtag
     3301 tacgtgacgg gggcggcgta cgcgcggcgg gcgatgcggc cgaggcggac ggcgcaggtg
     3361 aggcgggtgg cgctcccggc gcacccgggg gagccgccga tccgttcacc ccgttcatcg
     3421 ccgtccggtt cggcgcccgg gacgcccggg ggctccagtt cggcagcgac gtccgggtca
     3481 cccccggtgc ctccgggggg ccgctgctcg actgcgaccg cggcgaggtg gtcggcatcg
     3541 tcaaggggcg ccaccagcag gaccacgtcg gacttgcggt gccggtcacc gcgctgcgcg
     3601 gactgggccc cgaacacctc gtcgcgggcg ccaccgggct cggccccgac ccgtaccacg
     3661 cgctgatggg cctgcacgac cgctggcact ggagcggaca ggacctcggc cgccccggcg
     3721 ggcccacctg gttcgacgcc cagcacgcca tcatggcggg gcgcggccgg ctgtggggcg
     3781 tgcaggagcg gttgcaggcg ctcgacctgc tcgcccggct gcccgcgccg cgcgatccgc
     3841 tcgtcgtgga ggcggcggtc ggcgaggcgc tggaccgcgg cgagggcccc ggggcctggg
     3901 cgctgcgcac ctggcgggac ggccacggcg cgctctacca gggcagcgac ccgtacaccg
     3961 aactctgcgc cttcgtgcac tatctgcgga tcgtggcgca gcgggtcgcg gacgaggcgc
     4021 gcgaggcgcc cggcgaagcg gccgccgagg tgcgcgaaca cgccgcccac ctcgcggaat
     4081 tcgtccaggc caaggcggtg gtgctgcagc cgcaggaccg ccgccggatc ggccccgtgc
     4141 ggcgccggcc gcgctccgta ctcgtcgagt tcgagccgct gttctacgac gacggcgggg
     4201 gcgagctgtt caactggtcg gtcagcgagg ggtacggccg ggggcagtgg ctgcgggtgg
     4261 acgtccagga gtcggacggc ggggtgccct tcgagcaggc gcgggcgcag gtcctgcggc
     4321 ggctcggggc ccggctgctg cgcgccgacg gcgacgcggg accgggcgcc cgggtgcgtc
     4381 tggaggtcgc cgtccccgag gggtactggc acaccccggc cgaccgctgg gaggtcgcgg
     4441 cctcgacccg gcgcaccgca cgggcgcggc cggtgggccc ggggcgcgcg gtgatcctgc
     4501 gtgaccaggg gcgccgcgag gagatcgacc cggcctggct gcgccgctgg cagcgcgtag
     4561 ccgtcgcccc cgcactgcac gcgctgcgcg tcccgccgcc ccgccgggac ggcgcgggag
     4621 cggcggcggg ctccgccgac ggggtctggc ggctcctgga ggaggcggac gacggtgcgg
     4681 tgcccgcgct gtgccacacg gtcgcagacg ggaccggccg ggacgcggcc ggcgccgccc
     4741 tggacaccgg cttcccggcc ggactgtggt ccgcggccgg ccacggcgag cggtcctgcg
     4801 acgcatcctg cgaggaattc caccgcggcg tacgggagtt gctccagggg tccggctcac
     4861 tggcccgcct ccccgaactc gtccgggagc tgcgggcgaa ggccgcggag gagggcgccc
     4921 actgggcccg cgatctggtg ctcctctacg acgaccccga ggaccccatc ccgcagctct
     4981 tcacggcccg gccgcaggtg tcgccccggt gaccgcccgg ccggtacgca gccgccggaa
     5041 cgggcccccg ccggggggcc aagttgcgcc gccggccccc tcctccaggc cccttcgtcc
     5101 gtcggcccgc cccgtcctcc gtacgccccc gcgcgcccgt acgccctgca tgcgcccggc
     5161 cgccgcgacc ggacccggtg ccgccggata ccgcaagtca cgccccctgc ccggcacttg
     5221 gcggcgcgtg gcgacggtgc gggagaccgg aaaccgcccc cgtgagcttc cgtacggctc
     5281 gctcctgtca tagcgtcatg agacattgat tcgtgagtgt gcgtggcggg cgtgggggaa
     5341 gggccggggc ggacgccacc gtctccgggg cggtccgccg cgaccatcgc tccctgcccg
     5401 atggctggcc ggcgacgagg agttccgacg tgagcgactg gctcatctac cgcggcgcgg
     5461 gagcaccgca cgacggaatc gaacagctcc cgccgccccc gccctggcgc gatttcgacg
     5521 gcgggcccga catcccgccg ccgaccgccc tcgaccccgc ctccgaacgc cggctcggcg
     5581 tgctgcgcag ggaggccagc caccaccggc ccggcgagac cgagcgcgag ctggtcaacg
     5641 ccgcgctcta tctgcgcagg ccgctgctgg tgaccggcgc cccgggcagc ggcaagagca
     5701 ccctggccca ctcggtcgcc tacgaactcg gcctcggccg ggtgctcagc tggccggtcg
     5761 tcagccgcag cacgctccgc gacggcctgt acgcctacga cgccatcggc cgcctccagg
     5821 acctccagat cgcccgcgcc accccgccgc ggcccgccgg ccaggacgcc ccggagggcg
     5881 ccgccccgcc cgccccggac ccggatcaca ccgccgagcc cggcggcggt atcggccgct
     5941 acatccgcct cggcccgctg ggcacggccc tgctgcccgc cgccagaccc cgggtgctgc
     6001 tcgtcgacga gctggacaag agcgatatcg acctgcccaa cgacctgctc aacgtcctgg
     6061 aggaggggga gttcaggatt ccggagctgg agcggctggc cggatccgcc cccgaggtgc
     6121 gggtgctcag cgacgacggc gcgccggtga cggtgcgcga cggccgggtg cgctgccacg
     6181 ccttcccgtt catcgtgctc accagcaacg gcgaacggga cttcccggcg cccctgctgc
     6241 gccgctgcat ccacctccac atcccggcac cggacaagga gcggctcgcc gccatggtgc
     6301 gggcccactt cggcgagggc gccgccgagc gccacgcctc ggtgatcgac cgcttcctgg
     6361 accgcgagcc cggcgatgtc cgcgccgtcg accagctgtt caacgccatc cacctcacac
     6421 agcaggccgg ctggacggac caggacgagg aggagacccg acggcgcctg acggccgagc
     6481 tcatgcggcc tcttgaccgg acgaggtgaa cgcgatggct gtgggggagt ccggtccggg
     6541 acccctcgac cagctcgtcg cccggctgac cgcggcgggg ctgcccgccg acgcccgggg
     6601 gatggccgat gccctctggc tcgcccagtg gatcacgccg ggtgatcagc agaccgcccg
     6661 ggacgccacg ggcgaggccg gttccaccac cgccggatgc agcgatccgg ccactttcac
     6721 cgtgggcccg gccggacccg tggagcccgg cgcggcggcc gcacccgagc ccggcggccc
     6781 gtacgcgccc cgggacggcg cccgccgcac ggaggcggag ctgcggcccg cccgggaggc
     6841 gcggccaccc gatccggacc gccaccggct cggcgaggtc gccgtcccga tcgccgccgc
     6901 cttccccggg ctgctgccgc tccagcgcgc cctgcggccc gtccagcgct accacccgcc
     6961 ggtcgcaccg gtccgccggg agctggacga gcccgccacc gccgaactct ccgcacacgc
     7021 cgagacggtc atcccggtgc tgcgcggggt gcaccgccgg gaggccggtc tgcggctgct
     7081 gatggacggc tcgtcctcga tggccgtctg ggagcagatg ctgcacgatc tgcgccaggt
     7141 ctgtgagcag gtgggcgcct tccgcgacgt cacggtgcac tacctccacc cgcacggccc
     7201 ggacgtcggt gtcgccgcgg cccccggccc cggccggccg ctgcgccccg ccggtcagct
     7261 gcacgatccc accggccacc acctcaccct cgtcgtcagc gactgcgcgg gcccgctgtg
     7321 gcaggacggc cggctccagc ggctgctcca ccgctgggcg gccgacgcgc cggtcgccgt
     7381 cgtccagccg ctgccgcagc ggatgtgggc gcgcacccgg ctgcccgccg tcgccggtac
     7441 cctcgtccgc caccagggcc cgtaccaggc cctcggcttc cggcccgcca ggcgccgccg
     7501 ggcccccgcg gcgcccggac cggccccgga cgccggccgc ggccgcgcca tcccggtgct
     7561 ctccgcgacg cccgccgcgc tcggcgcctg ggccaggctg gccgcggccg atgccggcct
     7621 gtcgctgcgc ggcgccgcct gtgtggtccg cgccgaccac ggcgcggagc ccggcggccg
     7681 cccggccgag gcggagccct ccgcaccggc ccgcagggtc cgcgaattcg accggtccgc
     7741 ctcgccggac gcccggctgc tcgcggtgca cctgtcggcg gtcccgctgg cgctgccggt
     7801 catccagctc gtacagcgcg cgatgctgcc gcacaccgga ccggccgagc tggccgaggt
     7861 gctgctcggc ggcctggtca cccaactccc ggcggcggac cggtcgacgg gggaggcgga
     7921 cggggcgagc ggcccgtggt acgacttcgt gcccggcgta cgggacgaac tgctcggccg
     7981 gctgagcgcg ggcgaggccg cgctggtcct caagcactgc tcgctctacg tggagcgcac
     8041 cttcggccgc ggcgcccgca acttcccggc cgtcgccgtc gcgatgctct ccggcagcgg
     8101 ccgggagccg gagtccggcc gccgccccgt ccccgaaccg ttcgcccggg tctccgaaca
     8161 cgtcctgcgc cgcttcgaac cggcgctgcg gccgcccgag cagccgccct accccggcga
     8221 cggcccggcc gccgagggcg ccgccctcct ggagcgctac gaacagggca gcgccgtacg
     8281 ggacctgatc gaggccgtcc ggctgctgcg cgcggcggcc caccggctgc cgccggccgg
     8341 caccgacctg gcccgcgccc tgctgcacgc ctgggcgaag tggcgccagc ccgacgccct
     8401 ggcggaggcc gaacgcgcgg tgcgcgccgc ggagcgggcc accgcgccgg gcccggccga
     8461 cgaccaggag gtggggacgg ccggggagga agccggggcg gcggaggccg cgggccggga
     8521 ggcggagcgc acccgggcgc tgatcgtgct cggccgggtc cggtacgccc gcgcccggga
     8581 gcgtgcgggg gccggcgcac cggaggagga gcgcaccgcg ctggcggacg ccgcgcgcca
     8641 actgcacgcc gcctgcgggc tgatgagcct gctcgacccg cgtttcctgg agagcacggt
     8701 gctacggacc gaggtcctgc gccgggtggc cgccctgccg ccggccaccg gggcgcccgc
     8761 cgggcccggc gctcccgagg gggccggtcc gcccgacccc ctggacgagg cggaacggtc
     8821 cctcaccacc gtcctcgacc ggtggcccag cggcgagcag gttcccggcg aggtgtacgc
     8881 ggcgcgcggc ggcgtcctgc tggaccaggc ccgccgggcc ggccgcgtac gcccggacgg
     8941 gccgcgcccc gccgaggcgc tcgcggtccg tgccgcggac gacctcgaca ccgccaccct
     9001 gctgctgcgc cgccgtggca gcgccgccga ccgggtggtc tgcgagacgc tgctggatct
     9061 ggcggcggcc cgctcgctga ccggcggcag cgcgctgccg tccgtgctgc ccaccctgga
     9121 gcgggcccgc gcgatggcgc ggaccctcgc cgaaccggcc ctggaggccg acagcctgcg
     9181 ccggatcgcg gccgcgcagc gcgccgtgca cacccggacg ggcgcgccgg gccccctgga
     9241 cgaggccatc gccgcgctgg ccgccgcgca gcggctgacc gtccccgatt cccccgagca
     9301 cagcgcgctg ctcgccgagc gcggcgcggc gctgctgctg cgggcccggc tggagccgga
     9361 ccccgaaccg ggcaggcggc tggccaacga cgcggtgcac gtcctgcggg aggcgctggc
     9421 ccgggtcgcg ccgcaggatc cggagctggg cggccaccgg ctggccttcg gccgcgcgct
     9481 gcggctgcgc cacgaacgcc agccctcgct cgccgatctg cacgaggccg actggatcct
     9541 ggagctggcc gcccgcggct ccggggtgga gccgctcgtc gcggccgagg catggctcga
     9601 agtgggcgat gtccaactgc tgctggaccg ccgcttcgac tcccgggagc ggcacgaccg
     9661 ggccgccgcc tgctaccgcc gcgccgcgac gcacgcccac cggggcggca gtccgctgct
     9721 cgcggcccgc gcccaccacc ggcgcggcga ggtcctggag gagaccgcgg gcccccagcc
     9781 ggcgctgcac gcctaccgcg agagctggga gcagtggcag cacgcggccg aggagagcgg
     9841 cccggaggcc cagcgcaccc gcgcccggat gcgcgccctc gaatcccccg cctgaccgcc
     9901 cccgagcgcc ccgccggccg cctggtcgcg cggggcggaa cgtgtttctg tggacgggag
     9961 ttcgggaaca ccccgtccag ccggcccaga ggagagcagc gtggcgacga tccctcgcac
    10021 cgaaccggcc ggggccgccc cgtccggtcc gtcggccgag ccgctgccgg acttcggcgg
    10081 cgtggacgtg gccggtctgg cggcgcggac ggaccacgcg gtgctgggag aagtggcggc
    10141 gctgctgctg cgcaactggc cgtcggcgga tgacgcggtg gcgtactacg aagacggccc
    10201 ggaaaaactc ttgcactgac ggtcagaagc agttgttttt gggcaaacgc gcaagggaat
    10261 cggccagttg tcgcggcaat ccggttcctc aatcggtggt ggtgccatgt atcagtcagc
    10321 gggcgggagt tgacggttcc ggccgccgac gagagcaggg ggaccgcgcc gtggtggctg
    10381 tggagaccgg caggaccacc gtgcccttcc gccagttcgt cctgaagatg cacagccggt
    10441 gcaacctcgc ctgcacctac tgctacatct acgccggtcc cgaccgcagc tggcgcgccc
    10501 gcccgcggct cgtccccacc gccaccctcc gccagagcgc cctgcgcatc gccgaacacg
    10561 cccgcgccca ccgtctgcgc gagatccgca tcgacctgca cggcggcgaa ccgctgctca
    10621 ccggccccgg cccgctcctc gaccaggccg ccgccctccg cgccgcgctg cccgccggat
    10681 gcaccgccga attcggcgtg cagaccaacg gcaccctgct caccccggag ctgatcgaca
    10741 ccttgggcgc cgccggattc cgcctcggcc tcagcctcga cggcggcacc cccggtctca
    10801 accaccgccg cgtcgaccac gccggacgcc cctcctggcc cgccgtctcc cgcgccgtac
    10861 ggatgctcgc cgaccgcccc ggcgtcttcg ccggcatcct gtccaccatc gacatcacca
    10921 ccgacccggc cgaggtctac ggctcgctgc gcgccctcgg cccgcccgtg ctcgacttcc
    10981 tgctcccgca cgccaactgg tccagcccgc cgcccgcccc cctgggcccc gcgcgccgca
    11041 ccccgtacgg cgactggctg tgcaccgtct tcgacctctg gtgggacggc ggaccgggtc
    11101 cccgggtgcg gctcttcacc gagatcatcg gtctgctcct ggggcgcccc agctccagcg
    11161 aagcggtcgg cctctccccg gtcgtcgccc tcgtggtcga caccgacggc gccatcgaac
    11221 aggtcgactc gctcaagtcc acctacgagg gcgccgccgc caccggcctc gacgtcttcc
    11281 accacagctt cgacgaggcc ctcgcccacc ccggtatggc cgcccgccgg cgcggcccgg
    11341 acgcgctcgg cgcccagtgc cgcagctgcg ccctgatgcg ggtgtgcggc ggcggcaact
    11401 acgcgcaccg ctaccgcgcg ggccccagcg gattcgccaa tccgtccgtc tactgcgccg
    11461 atctggaacg cctgatccgg catatcgccg cccggctggc cgccgcgatg tagccccacc
    11521 gccccgccca caagatcgcc tgacgcaccg tcgtcacagg gagcgacact tgggctatcg
    11581 tgccaaacag cccgcccacg cacagcctct gcacgcgcgc accaccgcat cccagggaga
    11641 cggtcgcatg gccgatcagt cgacgttttc tgattcctct ggttcctcgg gtacctcagg
    11701 ttcctccggt tctccgggtc ccgccggcac ctccggtccc gcccgctccc acgaacacat
    11761 cacgatcagc tacgccggat tcaaccggcc ctgggcggcg tggatctccc accagctgga
    11821 gcagctgggc cacggcacca ccctgctgcg ctgggacccc gtcccggaga ccccgctcgc
    11881 cgacgagctg tccggactgc tcgccgccga gggccgcctc ctgatggtgc tggacgactg
    11941 gtacttccgg ctcggaccca agtcccacga ggagtggacg gacgcgctcc aggaagtggt
    12001 gacaccgcac gccgaccggt tcgccgccgt cagcgtcgcc acccagaagc tgcccgccac
    12061 cgccgccgtg ctgcgccccg ccgacctgcg cgacctggac gccgtcgagg ccaaccgccg
    12121 cgtcctgcaa cgcctcggcg tcacctccgc ccggcgcccg cgcggcgaac cgtcccccgg
    12181 cgccccgcgc ttccccaacg acccgcccga ggtctggaac gtcccgcgcc gcaacaaccg
    12241 cttcaccggc cgcgaccacg tcctggagga gctgcacgac aagctcgccg gcggcggcct
    12301 cggtgccggc ccgccgctgg agacccggat cgcgctgtac ggcatctccg gcgtcggcaa
    12361 gagccagatc gccgccgaat acgcccaccg cttcggcaac gactacgacg tggtgtggtg
    12421 gatcagcgcc accaaccgcg gcgccgcccg cgaacagctc gccgaactcg ccaaccgcct
    12481 gggcctcccc gtgggccatg aactcggcga ccgcatccgg gccgtccacg aggcgctgcg
    12541 ggtcggccgc ccctaccgcc gctggctgct ggtcttcgac agcgccgacg acatggagca
    12601 gatcgaggac ctcgtccccg acggccgcgg ccatgtcctg atcaccacgc tcacccgcga
    12661 ctggtcggga tccggcagcg cccaggagat cgaggtgcgg cccttcaacc ggatggagag
    12721 cgtcgcctac gccaggcgcc gggcgccgcg gctgacgccg ctggaggccg acctgctcgc
    12781 cgaggccgtc caggacctgc cgctgctgct cgcgcagacc gccgcctggc tggacgccaa
    12841 cccgatgtcc cccaaggagt acatcgagct gatccgccgc ggcgaaccca gcctggtcgg
    12901 catccgcatc tcctccgact acccgatggc cttccagacc agctgggcca taaccctcaa
    12961 caccctgcgc gaacgcagcc ccgccgcggt ggaactcctc aagctgttcg cgttcttcac
    13021 ccccgacgcc atccccgtac cgatgctcgc caaggcccgc cgcggcgatc tgcccgaaca
    13081 cctcgcggac ctcgccgccg agccgatcag ctggaacacc gcgctgcgcc ggctgaccga
    13141 atccaccgcc gtccgcctgg actaccagct ctccgaggag tccgacccgg ccgtcgagac
    13201 cgcgcagatg caccggctct accaccgttt cctgcgcagc gacatgaccg aggacgagcg
    13261 cgaccagatg tccgcgacgg cctgccgggt actggtcacc gccgacccgc agaacccctc
    13321 cgacacccgc tactgggagc gctacgccga actcatcccg cacctggagc cggccggcgt
    13381 cctggacagc cccgaaccgg ccgtccagga actcctgctc aactgcatcg aatacctgcg
    13441 ggtgcgcggc gagaaccgca tgggcctgcg gctgtgcgag cagttcctcg cgcgctggag
    13501 cacccgcatg gagcccaccc ggcgcaccat gctcgtcctc acccaccagc acgccaacat
    13561 gctgcgccgg atgggccgtt accgcgaggc cgaggcggtc ggcagcgcca tcgtcgacca
    13621 gctcgccgcc gaacgcaccc cggccgactc cgatctgctg cgcgcccagg acggcctggc
    13681 cggcacgctg atggcgctcg gctcgtacga ggaggcgtac accctcttcg acaccgtgtg
    13741 gcggacctac accgaggtgc tcggccccga ggcgccccgc accctctcct cgcggcacaa
    13801 cctcggctcc gccctcgccc tgctcggccg ctacgaggac gcgctgggca ccttccgcga
    13861 tgtgctggcc ttccgggagc gcgagctgcg cgcccgccac cacctcaccc tcaacgccgg
    13921 cgcctcctac tcccggatgc tgcggctgct gggccgctac cgcgacgcca cctccagcca
    13981 ggagctgaac gcccggctgc accggcaggt gatgggctcg cacacaccgc agacgctgcg
    14041 cgccgaacac gacctcgcgc tctgcctgcg ccgctccggt gaactcggcc gggccgacgc
    14101 cctgatggcc gacctcgtcg agcgctcccg ccgggtgcag gggccccggc accccgagac
    14161 gctgatggtg cgcgccgact acgccacctt cctgcgcgag cacggcgacc tcggacacgc
    14221 ccgcgacctc gccgaggaga tcgccgagcg ctaccgcgcc ctggccggtg cggaccaccc
    14281 gtacaccatc ggcaccctgg gcaacaccgg cctggcccgt ggcgcgttcg gggagctcac
    14341 cgaggcgctg gacatcgccg aacgggcact gggcgatatg acccggatca tgggacccga
    14401 ccacccctgg acgctgggct gcgcgctgaa caccgccgcc gcccgcaacc gcgtcgagga
    14461 cgaggacggc gcggtggagc tcagccacga caccctggag cgggccaagg cggcgctggg
    14521 cgggacccat ccgctgacgc tctccgccaa gacggcgctc gccgaggacc tgcgcacgct
    14581 gcgccgcggt caggaggcgg cgaagctgga gcaggaggcc gtccagcagc tcgccgagac
    14641 cctgggcgcc gagcatccgc acacgctgtc cgcccgccgc cgccggcgcc cgtactggga
    14701 cttcgaaccg cagcccgtgt aggcgcggcc atgtcctcgg cgcctcctcc caggacgcgg
    14761 cagccaaccc gaagggcccg tccccgcgag atgcggggac gggcccttcg gccactgccg
    14821 gtgccggtga ctacgcgtcg aagacctcgg cgatcagctg ggcctgctcc gcctggtgac
    14881 gcttggcgga accgacggcc ggggacgagc cgtgcgggcg cgagatgcgg cgcaggcgct
    14941 caccgtgcgg cacatcggcg ccgacggcca ggtccaggtg gtcgatcagg ttgagaccga
    15001 ggaacggcca ggcaccctgg ttcgccggct cctcctgggc ccacaggtac ttctcggcgt
    15061 tcgggaactt ggcgatctcc gcctggagct ccttgccggg cagcgggtag agccgctcga
    15121 tacggacgat cgccgtgtcg tgcgcaccgc gcttctcgcg ctcggcctcc aggtcgtagt
    15181 agaccttgcc ggcgcagaag acgaccttgc ggacggcggc cgcgtccacc gtggtgtcgc
    15241 cgatgacggg gcggaagccg cccgtcgtga actcttccgt cttcgacgcc gcggccttca
    15301 gacgcagcat cgacttcggg gtgaagacga ccagcggctt gtggtgcggg ttgtggacct
    15361 gccagcgcag caggtggaag tagttcgacg gcagcgtcgg catggcgacc gtcatgttgt
    15421 tctgcgcgca cagctggagg aagcgctcga tccgggccga ggagtggtcc gggccctggc
    15481 cctcgtagcc gtgcggcagc agcagggtga cgccggaggt ctggccccac ttctgctcgg
    15541 cggaggagat gaactcgtcg acgacggtct gggcgccgtt gacgaagtca ccgaactgcg
    15601 cctcccacat gaccagcgac tccggacggg ccagcgagta gccgtactcg aagcccatcg
    15661 ccgcgtactc gctcagcagc gagtcgtaga cgttgaaacg ggcctggtcc tcggagaggt
    15721 agagcagcgg ggtgtagtcc tcgccggtct cgcggtcgat gagcaccgag tggcgctgac
    15781 cgaaggtgcc gcggcgggag tcctggccgg cgagccggac cggggtgccc tccatcagca
    15841 gcgaaccgat ggcgagggtc tcgcccatgc cccagtcgat cgtgccgtcc tccacctggg
    15901 ccgcgcggcg ctgcaactgc ggcagcaggc gggggtggac ggtgacccgg tcggggatgt
    15961 tgacctggga ggaggcgatc cgcttgacga cctcctggga gatcgcggtg tccacggcga
    16021 ccgggaactc cgcctgcggc tgcggcacct cgggggtcat cggggccgtg gtcgcgtcgc
    16081 ggacctcggt gaagaccttc tccagctggc cctggaagtc ctgcagcgcc tgctcggcct
    16141 cttccagcgt gatgtcgccg cgaccgatga gcgattcggt gtagagcttg cgcaccgagc
    16201 gcttcttgtc gatcaggtcg tacatcagcg gctgggtgaa ggccgggttg tcggactcgt
    16261 tgtgaccgcg gcggcggtag cagatcaggt cgatgacgac gtccttgttg aacgcctggc
    16321 ggaactcgaa ggcgagccgc gccacgcgga ccacggcctc cgggtcgtca ccgttcacgt
    16381 ggaagatcgg cgcctcgatc atgcgggcca cgtccgtggc gtacatggag gagcgggccg
    16441 actccggcgg ggcggtgaac ccgacctggt tgttgatgac gatgtgcacc gtgccgccgg
    16501 tgcggtagcc gcgcagctgc gacatgttca gcgtctcggc gacgacaccc tggcccgcga
    16561 aggccgcgtc accgtgcagc tggaccggca ggaccgtgaa gtccgtgccg cccttgttga
    16621 tgaggtcctg cttggcgcgc gcgatgccct cgacgatcgg gtcgacggcc tccaggtggg
    16681 aggggttcgc ggccagcgag acggtgatct gctcgccgtc cagaccggtg aaggtgccct
    16741 cggcgcccag gtggtacttc acgtcgccgg agccgtgcat cgacttcggg tcgaggttgc
    16801 cctcgaactc ccggaagatc tgcgcgtacg acttgccgac gatgttcgcg aggacgttca
    16861 gccggccgcg gtgggccatg ccgatgacga cctcgtccag ccgcgactcg gccgcggagt
    16921 cgatgaccgc gtccagcagc gggatgacgg actcgccgcc ctccagcgag aagcgcttct
    16981 ggccgacgta cttcgtctgg aggaacgtct cgaacgcctc ggcggagttc agccggcgca
    17041 ggatgcgcag ctgctcctcg cgctcgggcg acttgtgcga gcgctcgacg cggtcctgga
    17101 tccacttgcg ctgcttgggg tcctggatgt gcatgaactc gatgccggtg gtgcggcagt
    17161 acgagtcgcg cagcacgccg aggatgtcgc gcagcttcat catggtcttg ccggcgaagc
    17221 cgccgaccgc gaactcgcgc tccaggtccc acagggtgag gccgtgctcg atgatgtcga
    17281 ggtcggggtg cttgcgctgg tggtactcca gcgggtcggt gtcggccatg acatggccgc
    17341 ggacccggta ggagtggatc aggtcgaaga cccgggccgc cttggtgacg tcgtcgtcgt
    17401 gcgagacgtc gatgtcgcgc agccagcgga ccggctcgta ggggatccgc agcgacttga
    17461 agatgtcgtc gaagaagtcg ttctcgccga gcagcagctg gctcatcacg cgcaggaact
    17521 cgccggaggc ggcgccctgg atcacgcggt ggtcataggt gctggtcagc gtcatgacct
    17581 tggagatgcc cagcttgttc agggtgtcct gggaggtgcc ctggaactcc gccgggtagt
    17641 ccatcgcgcc gacgccgagg atcaggccct ggccgggcat cagccgcggc acggagtgga
    17701 cggtgccgat gccgcccggg ttcgtcagcg acgcggtgac cccggtgaag tcgtccatcg
    17761 tcagcttgtt gtcgcgggcg cggcggacga tgtcctcgta cgcctgccag aactcgaaga
    17821 aggtcagcgt ctcggccttc ttgatggccg cgacgacgag ctggcggtca ccgttcggct
    17881 tgaccaggtc gatggccaga ccgaggttga cgtgctcggg cttgaccagc gtcggcttgc
    17941 cgtccttctg cgcgaaggag tagttcatcg acggcatggc cttgagggcc tgcaccatcg
    18001 cgtacccgat gaggtgggtg aaggagacct tcccaccgcg ggcgcgcttg aggtggttgt
    18061 tgatgacgat gcggttgtcg aacagcagct tcaccggcac cgcgcgcacg gacgtggccg
    18121 tcggcagctc cagggaggcg ttcatgttct tcgcgacggc cgccgagggg ccacgcagcg
    18181 tgaccagctc ggggccggcc ggcttggccg cggccggctt ggccgcagcg ggcgccggag
    18241 cgctcggcgc gggcttcgcc ggggccgggg cggcgggggc cgcgccctgc tgcgccgagg
    18301 agcctgcggg agcgctcgtg gcagcggggg cggccggcgt ggtcggggcg gccggaacgg
    18361 ccggggtcgc cggggcggcc ggtgcggggg cgatggcctc cgtcggctgg ctcggcgccg
    18421 cggcgccgcc cggcttgtag tcggcgaaga agtcccacca ggctcggtct accgagttcg
    18481 ggtcctggag gtactgctgg tagatctcgt cgacgagcca ctcattggga ccgaacgcgg
    18541 cggcagggtt ctttccctgc ccctcttggt cggtcgagat gctcgagctg ttgggggact
    18601 gtagcgacac ggcggcaacc gccctcttcc gcttcctaag gtggtggaca gcgggaataa
    18661 aggctacgcc tccccggacc gttaatgcag tccgggaggg tcactcgtcg cgcacgtcac
    18721 attcaccgct gggtttcaga gcatgacttg gcgggaaaca cacggggttt ggctgcgtgc
    18781 ggacagatcg cggcgccgtc gcaccccctg atcagtgctc ccaccaccga ccctacgcca
    18841 caggccgtca gctccgagct ggctccagcc ccggaagagt gacgcggata cggcagccac
    18901 gtggggattc ggccacgccg atccggccgc cgtgcagatc caccgcccag cgcgcgatgg
    18961 ccaggccgag gcccgtaccg ccgtcgctcc cggggccgga cgccgccggt ccgccgcggt
    19021 tgaaccgctc gaagacccgg tagcgctccg actcggggat gccggggccc tcgtcctgga
    19081 catccagctc cagactcccc ggaccctcgc cgcgccgtgc gtggaccgtg acccggccgt
    19141 gccgcgggct gtgcttgatc gcgttgtcga tgaggttggc gacgacctgg tgcagccgct
    19201 cggcgtcggc gtgcgcggtc agctcgggcg gggagacgtc gagatgcaga tggacgtccg
    19261 tgcgggtgtg ggtgcccgag ccggccagcg tcgcggcgcc gcggctcatg ttggcctcct
    19321 tgagcacccc ggacagatag ggccagacct cgaagcgggt ggtgtgcagc ggcaccacgc
    19381 cgttgtccag ccgggacagg tccaggaggt gctcgaccag ccggccgagc cgctcggtct
    19441 gcttcagcgc cgtccgcatc gtctccgggt cgggctggga gacaccgtcc acgacgttct
    19501 ccagcaccgc ccgcagcgcc gcgatggggg tgcgcagttc atgcgagaca ttggcgacca
    19561 gttccttgcg gtgggtgtcc accgcctcca gatcggccgc catccggttg aaggcgtccg
    19621 ccagatcgcc gaactcgtca cggcgcgccg agtgcacccg gcgggtgtag tcaccgtgcg
    19681 ccatcgactg cgtcacgtcc gtcatctcac ccagcggcgc ggtgaggctg ttcgccacga
    19741 actgggtgat cagcagcgac gcgatgatcg agaagatggt gatcacccgc agttcggtcg
    19801 ccgagtgcat cgcgacgaac accagcaggg tcgtgatgat caccgagacg atgaccagcg
    19861 cgccgagggc cgccttgacg gagcggtacg ggtccagcgg ccgcaatccg tcccagaccc
    19921 gcgcccagag cccccgcgcg ggcgcccgcc accgccgcgt catgcggccg gggtctccag
    19981 ggcgtagccg acgccgtgga ccgtgcggat gcgctcggcg ccgatcttgc ggcgcagcgc
    20041 cttgatgtgg ctgtcgaccg tgcgcgtccc ggaggcgtcc gcccagtccc agacctcggc
    20101 gagcagctgc tcgcgggaga gcaccgcgcg cggggtgttc gccaggcaga ccagcaggtc
    20161 gaactcggtc ggggtgaggt ggacgtcggt gccgcgcacc cgcacccggc gctgggcgtg
    20221 gtcgatctcc agctcaccga ggcgcaggat gccgctgcgc ggggtgtgcg cggccagcgc
    20281 ggcgcgctcc acccggcgca gcaggacgtg cacccgggcg gccagctcgc gcatcgagaa
    20341 cggtttggtc atgtagtcgt cggcgccgac cccgaggccg acgagcatgt cggtctcgtc
    20401 gtcgcgggcg gtgagcatca tcaccggcac cggccgctgg gcctggaccc ggcggcacac
    20461 ctccaggccg tcgaagcccg gcagcatcac gtccaggacc agcaggtccg gctgccacgc
    20521 ctcggcggtg tcgacggcgg ccgggccgtc ggtggccgtc tgcacctgga agccctcggc
    20581 gcgcagccgg gccgcgatgg cgtcgacgat cgtcgggtcg tcctccacga ccagcacccg
    20641 gcgctgggcg cccggcgtgg ccgcggcggt gccgccctgg ctggtctgag tctggtccat
    20701 cgcccgcccc tgcatgtccc cgtgtgcccg gtcgcgtagt gctgggcagc agcgtaaggg
    20761 caggatgtga ccgtcggcta cgcgccctta cgcgcgagat gcaccacgtc ggggacgcct
    20821 cggacaaccg ggatctcttt cgtacgtacc ccgctgaacc cggcatttcg aagggcctgt
    20881 tcgaaaccgg gggacggctg agcggaccag acggcgagca cgccgcccgg tgccagtcgg
    20941 tcccggcagg cggcgaggcc ggcgggggag tagaggctgc cgttgccgtc ggtgacggtc
    21001 cagtcgggcc cgttgtcgat gtccaggcag agtgcgtcgt aggtgtcccc gtccgctccg
    21061 gtacggaggt acgcgacgag atcggtggcc aggatctcgg tgcgcggatc ggccagcgcc
    21121 gccgccgaga ccgccgacag cggacccgtg cggtgccagt cgatgaccgc ggcctcccgt
    21181 tcgacgacgg cgatccggcc ccagcgcggt tcggcggccg cccgcgccag tgagaagccg
    21241 acgccgagac cgccgatcag gacggcgggc cgcggccggg cggccggcag ctcggccagc
    21301 gcggcgtcga tcagcagccg ctcggaacgg ccgtcggagg tgtccatcag gaagcagccg
    21361 ttggcgatga tctcgtagac cggcggtgcg ccggggcccc cgtcccgctg ccgcagtgcc
    21421 acctcgccgt acgggccctc ccggcggtcg agggtgcgtg gcgcctccgg gaggccggcc
    21481 gtggagtgct gggggagcgc gggagccatg cgg
//
