LOCUS       NZ_NDXL01000001        10942 bp    DNA     linear   CON 27-APR-2024
DEFINITION  Streptomyces kasugaensis strain BCRC 12349 scaffold1, whole genome
            shotgun sequence.
ACCESSION   NZ_NDXL01000001
VERSION     NZ_NDXL01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces kasugaensis
  ORGANISM  Streptomyces kasugaensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Comparative genome analysis for kasugamycin overproduction
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Comparative genome analysis for kasugamycin overproduction
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030,
            China
REFERENCE   4  (bases 1 to 5071473)
  AUTHORS   Liu,X., Zhu,C., Bai,L. and Jiang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2017) School of Life Sciences and Biotechnology,
            Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. May-2015
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 188.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002261115.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 02:42:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,951
            CDSs (total)                      :: 7,863
            Genes (coding)                    :: 7,657
            CDSs (with protein)               :: 7,657
            Genes (RNA)                       :: 88
            rRNAs                             :: 6, 4, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 4, 4 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 87 of 206
            Pseudo Genes (incomplete)         :: 170 of 206
            Pseudo Genes (internal stop)      :: 23 of 206
            Pseudo Genes (multiple problems)  :: 65 of 206
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:37:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 35443
            Orig. end                         :: 46385
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NDXL01000001.1.
            Bacteria and source DNA available from BCRC.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10942
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10942
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10942
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5942](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5942
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(1033..1782)
                     /locus_tag="B7024_RS00145"
     CDS             complement(1033..1782)
                     /EC_number="4.2.3.153"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606395.1"
                     /locus_tag="B7024_RS00145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="WP_094790674.1"
                     /transl_table=11
                     /translation="MLLLISPDGVEEALDCAKAAEHLDIVDVKKPDEGSLGANFPWVIR
                     EIRDAVPADKPVSATVGDVPYKPGTVAQAALGAAVSGATYIKVGLYGCTTPDQAIDVMR
                     GVVRAVKDYQPDAFVVASGYADAHRIGCVNPLALPGIALRSGSDAAMLDTAVKDGTRLF
                     DHVPPDVCAEFVRLAHEADLLAALAGSVKVADLGELTRIGTDIVGVRGAVCEGGDRTTG
                     RIQPHLVAAFRAEMDRHAREYAAAAAN"
     misc_feature    1739..1754
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.79"
                     /tool="antismash"
     gene            complement(2073..2696)
                     /locus_tag="B7024_RS00150"
     CDS             complement(2073..2696)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017949441.1"
                     /locus_tag="B7024_RS00150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_094790675.1"
                     /transl_table=11
                     /translation="MVRQERAARTRAALVRAAAEEIDRRGYDGTSLNKVINAAGISMGA
                     LTFHFSTKSELADEVQSQGSHAARTVADHVTAESGPALQAVIDLTLELARLLEEDPVVR
                     SAARLARERPGSPAWSAAWLPAVHSLLAQAQRDGQLHPAVLPETIAALVVHLVAGTETY
                     ARARSVTTDTARASAVVQLGRIWQLALSGISPREPSDGPPAPRG"
     misc_feature    3004..3013
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.58"
                     /tool="antismash"
     gene            3074..4300
                     /locus_tag="B7024_RS00155"
     CDS             3074..4300
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004994169.1"
                     /locus_tag="B7024_RS00155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_052855304.1"
                     /transl_table=11
                     /translation="MAAHASGQLPAPHGQVPAATFVEELFGHLPRADQRRWALAYMQGL
                     LSTPGKKSVRRLAATISTSPTASQSLHQFINASPWNWKPARQELLRWVEQRAQGQAWTI
                     GLALLPKRGEHSVGVHRRFVQESGRTLNCQVGVGAFLSTGGMELPVDWRLLLPDQWRQD
                     PQRRLRARIPDAAAHDRQVWSHILDLADSLASRTRTTLPPLVADMSECTDTAALMLGLS
                     QRGRDFLLTVPNSLPVLSCARSAGRQPGGPGPAAPLGARQLLSLSGSGRHPHTATVSTH
                     DGATRPVRILSGLARLPQAGPCTGSRQHIYRLFTELRPAGAQLSRMWVTNMTNRRMDEL
                     LPLTGLPTVTATTLHDLGTHFGLLDFEGRSFPGWHHHMTLLSAAYAYHRLAGSTLLPPH
                     AQASGLRSA"
     misc_feature    4900..4920
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.27"
                     /tool="antismash"
     gene            5001..5942
                     /locus_tag="B7024_RS00160"
     CDS             5001..5942
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979781.1"
                     /locus_tag="B7024_RS00160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_094790676.1"
                     /sec_met_domain="AfsA (E-value: 1.7e-19, bitscore: 67.7,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDSARRDAASGTALFSRVPRELVHKTAPDEVLLTGWRQEAEDHF
                     VVTASWPEQHSFYVPVAGARDPLLAAESVRQAIPLLCHGAYGVPQGHRQIWDHFACALH
                     PDALSADEGPATIELDVSCSDVRRRGSRLAAMTMQVRLMSDGACMGVAHARFTNQPPAI
                     YQRLRGEYADLEAAVSRTIPLAPPISPKQVQRSRFRDVVLSPTNTAQVSQLRVDLAHPV
                     LFDHPVDHAPGMLLLEAARQATHAVAHPHDVVLTGMDCAFSHYAELDAPCWIETSALGN
                     ELMGRLAVQVVARQFGREVFSALITAEHTVRT"
     misc_feature    5238..5240
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6226..7188)
                     /locus_tag="B7024_RS00165"
     CDS             complement(6226..7188)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742094.1"
                     /locus_tag="B7024_RS00165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_094790677.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 6.3e-10, bitscore:
                     36.6, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTWKVALTGATGFIGSAVLTTLLETPRAGRDITVRALARTPPTG
                     PAVPGLEWILGDLTAAESLPALVAGSDVLIHLASYIGGDEDRCTQVNVHGCAALMAEAR
                     RAGVQRIIHLSTAAVYGMGPHRGIAVNEVAPAPVSPSSRTRWEGEGKALAAGGTVLRPG
                     LVLGRGDRWVVPAYAELLERVPARWDDGRGLLSLVDVGDLARLITALAYLTEPPRGIFH
                     AGHPVPVSNGELLAALAAHKVLPSPGRENWSWQTCLEQLRKHPGKMSERQFSLLARDHW
                     YRSDSIWEAAGCPAGPGALSRLGEAAEWYRAHLATPPAR"
     misc_feature    complement(6997..6999)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(7185..7808)
                     /locus_tag="B7024_RS00170"
     CDS             complement(7185..7808)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014628787.1"
                     /locus_tag="B7024_RS00170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_052855301.1"
                     /transl_table=11
                     /translation="MARQRQERAVRTRDALIQAAAETFDENGFSGAGINRILERAGVTP
                     GAMYFHFKSKEDLARAVIVEQAAALRLPEQPSGIQQLVNLTLYLAGEMQRNVRFRAGVR
                     LAVDQTEGGVQDFTVYEWWGEKFRQELLAARARDELLEDVDEAECARVLVAAYTGTQLM
                     SQLSTGRADLPQQIVAMWRYLLPGIARPRTVSRVTVDAALSVAA"
     misc_feature    7830..7843
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.89"
                     /tool="antismash"
     misc_feature    complement(7902..7915)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.03"
                     /tool="antismash"
     gene            complement(7949..8899)
                     /locus_tag="B7024_RS00175"
     CDS             complement(7949..8899)
                     /EC_number="2.5.1.-"
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /GO_process="GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016828701.1"
                     /locus_tag="B7024_RS00175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cysteine synthase family protein"
                     /protein_id="WP_094790678.1"
                     /sec_met_domain="PALP (E-value: 2.3e-69, bitscore: 232.0,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVARTAECRIADGLEDLIGNTPLVRLHLEGAHPDVEVLAKLESVN
                     PFSSSKDRSALHMMRGAEARGELVPGGTVVESTSGNTGVALAALAAARGYRCVIVLPDS
                     AMPERIGLLRALGTEVVQTPSEDGFQGAIDKAEEINARTAGSWYARQHENDDNVLAHYE
                     TTGPEIWADTAGTVDVLVAGVGTGGTLTGTARYLKERNPALEAVAVEPESSPVLSRGYG
                     GLHRIPGLNGGFVAATTDRTLIDHVFTCSDEDAVRAAKSLAARQGLFVGVSSGAAVHGA
                     ARLARLPRYAGRTIVTVLPDTGERYVSLWNEGAAP"
     gene            complement(9028..9636)
                     /gene="epsC"
                     /locus_tag="B7024_RS00180"
     CDS             complement(9028..9636)
                     /GO_function="GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /gene="epsC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018893098.1"
                     /locus_tag="B7024_RS00180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="WP_052855299.1"
                     /transl_table=11
                     /translation="MHTVPGLLRTMREDLDVVIERDPSISTRREGVLHPALPALWAHRV
                     AHRLHRRGRRMSARVIAYFARRHTGVEIHPGARIGRRVFIDHGSSVVIGETAVVGDDVT
                     LFHQVTLGAVGWWNDNHRAPGERRHPVVGNGVVLGANATVLGPVTIGDGALIGSMATVT
                     KDIPPGSRVYAPASVVKSVGGSAGPAGDPVRLLASTGSW"
     gene            complement(10016..10567)
                     /locus_tag="B7024_RS00185"
     CDS             complement(10016..10567)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /locus_tag="B7024_RS00185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="WP_052855298.1"
                     /transl_table=11
                     /translation="MARIALISGSLRAESSNSAVVATIRKIIEGRAGGHEATVVPLHDF
                     PPFDQDVERAGHPAPLESARGQVSGADALIVCSPAYNGYPPGVLKNALDWLSRPGGESP
                     LTDLPTAVLSASPGQAGGANVQPHVRRILANCGAAAVECDEVAVGDAISLRTSEGLIED
                     PATVSRLEKLVDAVLAAAAR"
ORIGIN
        1 gccggtggcc gccggccgcg gctcgtgcgc cgtccgcgag ggcctgggcg gtgtagtgct
       61 cgacccgggc cagttggggg gcgttgttga ccggtcccag ttgcgagcgc gggtcgagtc
      121 cggctccgac gacggcggtc ttcgcgcgct gcgcgagggc ctcgaccacg tgggagtgga
      181 gccgggccgg ggcgtagacg cgtttgacgg ccatgcagac ctgtccgcag ttgcggaacg
      241 ccgcccagaa cagtcggtcg gcgatccgtt ccacgtccac gtcctccagc aggatggcgg
      301 cgtcgttgcc gcccagttcc agggtgaccc gggcgagcga ggccgccgcg gccttggcga
      361 cggcccgtcc ggtggggatc gaaccggtga aggtcacgtg gcggattccg gggtgggcgg
      421 cgaggcgggc gccgaggggt tcgcggccgg tgatgatcgt caggacgtcc tcggggaggg
      481 cggtggccag gacggtcccc agcagccggg tggcgagggg ggtggacggg gagggcttga
      541 gcaccatcgt gttgcccgcg gcgagcgcgg gcgcgaactt cgccgatgcg agctggaggg
      601 gaaagttcca cgggacgatc gcggcgacgg gcccgagcgc tcgccagcgg agctcgctgt
      661 gtaccggccg gccgtccgtg accggccggg gcgcggggtc cagctcggcg aagtagcgca
      721 ggcgggccgc cgtgcgggcg acctccgcgt acgactcggc caggggtttg ccctgttccc
      781 gggtgagcag gggggcgagg tcggtgccgg ccgcctccac ggcgtcggcc gccgcgcgca
      841 gcgcggtggt gcgggcggcg gggtcggccc gccagccgcg ccatgcctgg tgggcgcggt
      901 cgatgacggc gtccagttcg ggtggctgct ggtcgggggc ctcgtcgaat gcctcgcccg
      961 tcgccgggtc gaggacggcg aggtggcggg tcccgggggc gcggcggggc cggggtgtcg
     1021 gcatgccggc ggtcagttcg cggcggcggc cgcgtattcc cgggcgtggc ggtccatctc
     1081 cgcccggaag gcggctacca ggtgtggctg gatccttccg gtggtgcggt cgccgccctc
     1141 gcagaccgcg ccgcgtactc cgacgatgtc ggtgccgatg cgggtcagtt cgccgaggtc
     1201 tgccaccttg acgctgcccg cgagggcggc gagcaggtcg gcctcgtggg ccagccggac
     1261 gaactccgcg cagacgtcgg gcggaacgtg gtcgaagagc cgtgttccgt ccttgaccgc
     1321 ggtgtcgagc atggccgcgt cggagccgga gcggagcgcg atgccgggca gggcgagcgg
     1381 gttgacgcag ccgatccggt gggcgtcggc gtagcccgag gcgacgacga acgcgtcggg
     1441 ctggtagtcc ttcaccgccc ggacgacgcc tcgcatgaca tcgatggcct ggtcgggcgt
     1501 cgtgcatccg tagaggccga ccttgatgta cgtggctccg gagacggctg cgccgagcgc
     1561 cgcctgggcc accgtgccgg gcttgtacgg tacgtctccc acggtggcgg ataccggttt
     1621 gtccgccggg accgcgtcac ggatctccct gatgacccag gggaagttgg cgccgagtga
     1681 gccttcgtcg ggtttcttga cgtcgacgat gtcgaggtgt tccgccgcct tcgcgcagtc
     1741 gagggcctct tcgacgccgt ccggggagat gagaagcaac aaggtggacc ccttccaccg
     1801 catgtccacc cggccgaggc gcaggtgtgc ggagtcgtct ggatcacttg cggtccgcac
     1861 atccttgccg ctgcccgtgg ccgccggtag ggcgcgcgga ggccgaacgg cactgtcaca
     1921 gcgtgcccgg tgcgccgtcc acgccgggga gattcggcca tgtgcggggg tggacaggct
     1981 gcacggcgtc gggccggcct gagccgcggc gacggcacgg gcttacggaa ggagcgccgt
     2041 cttccggtgc gtgatgccgg gtccggccgg ggtcagccgc gaggtgcggg gggcccgtcg
     2101 gacggctcgc gcgggctgat gccggacagg gcgagctgcc agatcctgcc cagttgtacg
     2161 acggcgctgg cgcgcgcggt atcggtggtg accgaacgtg cccgtgcgta ggtctcggtg
     2221 ccggcgacca gatggacgac gagtgcggcg atggtctcgg gcagtacggc cggatggagc
     2281 tggccgtccc gctgggcctg tgcgagcagg ctgtggacgg ccggcagcca ggcggctgac
     2341 catgcggggc ttcccggccg ttcgcgggcg agccgggccg ccgagcggac gacggggtct
     2401 tcttccagga gccgggcaag ttcgagggtc aggtcgatga cggcctgtag ggccggcccg
     2461 gattccgccg tgacgtggtc cgccacggtg cgggcggcgt gggatccctg cgactggacc
     2521 tcgtcggcga gttcgctctt ggtggagaag tgaaaggtca gtgcgcccat ggagataccg
     2581 gccgcgttga tgaccttgtt cagcgaggtg ccgtcgtagc ccctgcggtc gatttcctcg
     2641 gctgcggccc gcaccagggc ggcgcgtgtg cgggccgccc gctcctgcct caccacggct
     2701 gcgctccgaa gtgcggggca aggccgttcc tcggacggct cgcctgcgcg gcggggaggt
     2761 cgcaaacggt ggtcactgcg gacggcgggg cgggtcggcg cacccgtcgt gcattcacca
     2821 aaacagttct ccttcggtca ttccggctgg tgagacggcc agcgtgctcg ctgtgtgacc
     2881 gggtgacaac tccgacttga cgaagtgtcg cgtttcggcc gatcagcggt gtgtgcgggg
     2941 catatgacct gccgaagtag gagtcaccgg ggcccgggag cagaaaacaa agagattgtt
     3001 ctctattatt ttctggagtc tcggacgggc cggtcgtacc gccgaccggc accaggggga
     3061 gggaacgcac acgatggctg cgcatgcttc gggtcaactg ccggcgcctc acggccaggt
     3121 acctgccgcc acattcgtcg aggaactctt cgggcacctg ccgcgggccg accagcgccg
     3181 ctgggccctc gcctatatgc agggactgtt gagcacaccg ggcaagaagt cggtgcgccg
     3241 cctggccgcc accatctcca cctcgcccac ggcctcacag tcgctgcacc agttcatcaa
     3301 cgccagcccc tggaactgga aacccgcccg gcaggaactc ctgcggtggg tcgaacaacg
     3361 cgcgcagggc caggcatgga cgatcggtct ggcgctcctg cccaagcgcg gcgagcactc
     3421 cgtcggcgtg caccgcagat tcgtgcagga atccgggcgc accctcaact gccaggtggg
     3481 cgtcggggcg ttcctgtcga ccggcggaat ggagcttccg gtcgactggc gtctcctgct
     3541 tcccgaccag tggcgccagg atccgcaacg gcgcctgcgc gcccggatcc cggacgccgc
     3601 cgcccacgac cgtcaggtgt ggtcgcacat cctggatctg gccgattcac tggcttcgcg
     3661 cacccgtacg actctgccgc cgctggtggc ggatatgagt gaatgcacgg atacggcggc
     3721 gctgatgctc gggctgagcc agcgcgggcg ggacttcctc ctcaccgtcc ccaactccct
     3781 ccccgtcctg tcctgcgccc gctccgcagg ccggcagcca ggcggaccgg ggccggccgc
     3841 cccgctcggc gcccgtcagc tgctcagcct cagcggcagc ggccgccacc cgcacacggc
     3901 caccgtgagc acccacgacg gagcgacccg gcccgtcagg attctgagcg gcctggcccg
     3961 gctgccgcag gccggtccgt gcacggggag ccgccagcac atctaccggc tgttcaccga
     4021 actgcggccc gccggggcgc agctctcccg tatgtgggtc accaacatga cgaaccggcg
     4081 tatggacgaa ctgctccccc tcaccggcct gcccaccgtg acggcaacca ccctgcacga
     4141 tctcgggacc catttcggtc tcctcgactt cgagggccgg tccttccccg gctggcacca
     4201 tcacatgacg ctgctctccg ccgcgtacgc ctaccaccgc ctcgccggtt cgacgctcct
     4261 gcccccgcac gcccaggcgt ccggcctgcg ctccgcctga actccccagc tatgagggcg
     4321 ccgaggcgtc acccggtggc ggtctccccg gcccgctccc acccggccac gcggccgacg
     4381 ctcacttggt ggcggccctc ggtgcggagg cgtacgcggt gggccggatc gggcccggcg
     4441 ttgccgtcgg caggtccggg ccgcacacct gcccggggtc accacgtggt caacccggct
     4501 cggggtaggg gccgttggcg gtggccatcg gtccgcagca ggtcgcgcgc cgcggctcct
     4561 cgccatcgcg gccggcctcg aaggggcgtc gggccgacgg ggccgacggc ccatgtcccg
     4621 ctgatcgccg gacgggttcg ggacggaatc cgccggaacg ggcgactccg gggcggatcg
     4681 gcacttcgcg gaccggtgtc cgcctcggat cacggacggc tgcgttcccc tgtcatccgg
     4741 tgtttcgtgc gagccgggag cggacccagg tccgccgaag gtccctgaac accccgccca
     4801 gcagcatgaa tgcctgcgag gtgccgttcc gcccaactgc acgccacacg ttcgcacttc
     4861 ggctccacca gaggcggtgc tgccaccttc tcgacaaaca aaaagaacac cctctatgtt
     4921 attggcgcgg cggtcaggtc actcgcccac gggggccgag ggcgcgccgc gagtgatttg
     4981 cctaaggggg aagcaagggc atgtccgatt ccgcacgccg tgacgctgcg tccggcaccg
     5041 ctctgttcag ccgggtgccc cgggaactgg tgcacaagac ggcgcccgac gaggtcctgc
     5101 tcaccggctg gcgtcaagaa gccgaggatc acttcgtcgt cacggcctca tggccggagc
     5161 agcacagttt ctacgtaccg gtggcggggg cccgcgaccc cctgctcgct gccgaatcgg
     5221 tgcgccaggc gatcccgtta ctgtgccacg gcgcctacgg cgtgccgcag gggcaccggc
     5281 agatctggga tcacttcgcc tgcgccctcc accccgacgc gctgtccgcc gacgaagggc
     5341 cggccaccat cgagctggac gtctcctgct ccgatgtacg gcgccggggc agcaggctcg
     5401 ccgccatgac gatgcaggtg cgcctgatga gcgacggcgc ctgcatgggg gtggcccatg
     5461 cgcggttcac caaccagcct ccggccatct atcagcgcct tcgcggggag tacgccgatc
     5521 ttgaggccgc ggtgtcgcgg accattccgc tggccccgcc gatatccccg aaacaagtgc
     5581 agcgcagccg gttccgggat gtcgtcctct ctccgacgaa cacggcgcag gtgtcccaac
     5641 tccgcgtcga cctggcccac ccggtcctct tcgaccatcc ggtcgatcac gcgccgggca
     5701 tgctcctgct ggaggccgca cgccaggcga cgcacgcggt cgcccatccg cacgatgtgg
     5761 tgctcacggg aatggactgc gccttctcgc actatgcgga actcgacgcc ccgtgctgga
     5821 tcgagacatc cgccctcggc aacgagctga tgggccgcct cgcggtacag gtggtcgccc
     5881 gtcagttcgg ccgagaggtc ttctcggccc tgatcacggc cgagcacacc gtgcggacct
     5941 gaggggccgg cgggcgcccg cgatggctga gcggccgtcg gggcggcggg gctcaccccg
     6001 ttggcggcag ggatcaggca tgtctcctgt ccgtgtccct ccgcccgtcc gtccgtccgc
     6061 tcgtgcgtga tcggcggtgc ggcgcttgtg gcggacttcc cgtcgtcttc cggtgccacg
     6121 acggcggaac ggcaacggtc agggctccgg tgcgggtgcg caccggagcc ctgacgtgag
     6181 tcgctcgcgt cgccgggtgc gtcccggacg gcctgccgtg cggcctcagc gggcgggtgg
     6241 cgtggcgagg tgggcccggt accattcggc ggcttccccg agccgggaga gcgcgccggg
     6301 gcccgcgggg cagccggccg cctcccagat cgaatcgctg cggtaccagt gatcgcgtgc
     6361 gagcagcgag aactggcgct cgctcatctt gcccgggtgt ttccgcagtt gttcgaggca
     6421 ggtctgccag gaccagttct ccctgccggg cgacggcagc accttgtgtg cggccagcgc
     6481 cgccagcagc tcgccgttgc tgaccggaac cggatggccg gcatggaaaa tcccgcgggg
     6541 cggctccgtc agatacgcga gggccgtgat cagccgcgcc aggtcaccga cgtccacgag
     6601 tgacaggagg ccgcgcccgt cgtcccagcg cgccggaacc ctctccagca attccgcgta
     6661 ggcgggcacc acccaccggt cgcctctccc cagaaccagg ccgggccgca ggacggtccc
     6721 accggcggcc agagcctttc cttcgccttc ccaccgggtc ctgctggaag gcgaaaccgg
     6781 tgcgggcgcc acctcgttca ccgcgatccc ccggtggggc cccatcccgt ataccgccgc
     6841 cgtggacaga tggatgatcc gttgcacccc ggcgcgcctc gcctcggcca tgagtgccgc
     6901 gcagccgtgg acgttgacct gcgtgcagcg gtcctcgtcg ccgccgatgt acgacgccag
     6961 gtggatcagt acgtccgacc cggcgaccag cgcgggtaag gactccgcgg cggtcaggtc
     7021 gccgaggatc cattccaggc ccggtacggc tgggcccgtg ggtggtgtgc gggccagtgc
     7081 ccgcacggtg atgtccctgc ccgcccgggg tgtttcgagc agtgtcgtca ggaccgccga
     7141 cccgatgaag ccggtggcgc cggtgagcgc caccttccac gtggtcatgc ggccacgctg
     7201 agggcggcat cgaccgtcac ccgggagacc gtccgcggac gggcgatgcc gggcagcagg
     7261 tagcgccaca tcgcgacgat ctgctgggga agatccgccc ggccggtgga gagctgggac
     7321 atcagctggg tcccggtgta ggcggccacc aggacccgtg cgcactccgc ctcgtcgacg
     7381 tcctccagga gttcgtcccg ggcgcgggcg gcgaggagct cctgccggaa tttctcgccc
     7441 caccattcgt acaccgtgaa gtcctgtacc ccgccttcgg tttgatcgac cgccagccgt
     7501 acaccggccc ggaagcgcac attgcgctgc atttctccgg cgaggtagag ggtcaggttc
     7561 accaactgct ggatgccgct gggctgttcg ggcagacgca gcgcggccgc ctgctccacg
     7621 atgaccgctc tggccaggtc ctccttcgat ttgaagtgga agtacatggc ccccggtgtg
     7681 actccggcgc gttcgaggat cctgttgata cccgccccgg agaagccgtt ctcgtcgaaa
     7741 gtctcggcgg ctgcctggat gagtgcatcg cgtgtccgga ctgcccgttc ctgcctttgg
     7801 cgtgccacgt gctggtcctc gatctctgaa tagtacaaaa agaatgacct ctatgttatg
     7861 tccggaacgg ccttcgccgg tacccgcgtt cgcggggcgg ccgaacaggc gttggcgtgg
     7921 aaggcgccgc ggtgcgggac cggcgcggtc acggggcggc cccctcgttc cagaggctga
     7981 cgtagcgctc gccggtgtcg ggcaggacgg tcacgatcgt ccgccccgcg taccggggca
     8041 ggcgggccag ccgggcggcg ccgtggaccg ctgcgcccga ggagaccccg acgaacaggc
     8101 cctgccgggc ggccagggac ttggcggccc ggacggcgtc ctcgtcgctg caggtgaaga
     8161 cgtggtcgat gagcgtgcgg tcggtggtgg cggcgacgaa gccgccgttc aggccgggga
     8221 tgcgatgcag cccgccgtag ccgcgggaga gaacgggcga ggactccggc tcgacggcca
     8281 ccgcctccag ggcggggttg cgctccttga ggtagcgggc ggtgccggtg agggtgccgc
     8341 cggtgccgac gccggcgacc aggacgtcca cggtcccggc ggtgtcggcc cagatctccg
     8401 ggcccgtcgt ctcgtagtgg gccagcacgt tgtcgtcgtt ctcgtgctgg cgggcgtacc
     8461 acgagccggc cgtgcgggcg ttgatctcct cggccttgtc gatggcgccc tggaagccgt
     8521 cttcgctggg ggtctggacg acctcggtgc ccagcgcgcg cagcagcccg atccgttcgg
     8581 gcatggcgct gtcgggcagg acgatgacgc agcggtagcc gcgggcggcg gcgagggcgg
     8641 ccagcgcgac gccggtgttg ccggaggtgg actccacgac cgtgccgccg gggacgagtt
     8701 cgccgcgcgc ctccgcgccc cgcatcatgt gcagggccga gcggtccttg ctgctggaga
     8761 aggggttgac cgactccagc ttggccagca cctccacgtc cgggtgggcg ccctccagat
     8821 gcagccgcac caggggggtg ttgccgatca agtcctcaag accgtccgcg atccggcact
     8881 cggcggtacg ggcaaccatg agtgaactcc tgtcagttgt acgcgtactg cgcgacggaa
     8941 gaggcgggga ggacggcggg gcgaggcggt gcgcccggcc ggggtgccgg ggcgggggcg
     9001 gtggctcggc tcagcggggc ggggggatca ccacgagccg gtcgaggcga ggagccgtac
     9061 ggggtcgccg gccggcccgg cggagccgcc cacgctcttg accacggacg cgggcgcgta
     9121 gacccggctg ccgggcggga tgtccttggt caccgtggcc atcgagccga tcagtgcgcc
     9181 gtcaccgatg gtcaccggcc ccagcaccgt cgcgttggcc cccaggacga cgccgttgcc
     9241 gacgacgggg tgccgccgct cgcccggggc gcggtggttg tcgttccacc agccgacggc
     9301 gccgagtgtg acctggtgga agagggtgac gtcgtcgccg accaccgccg tctcgccgat
     9361 gacgaccgac gagccgtggt cgatgaagac gcggcggccg atccgcgcgc cggggtggat
     9421 ctccacgccg gtgtggcggc gggcgaagta ggcgatgacc cgggcgctca tgcggcggcc
     9481 gcggcggtgc aggcggtggg cgacgcggtg ggcccacagg gcgggcagcg cggggtgcag
     9541 gacgccctcg cggcgggtgc tgatggacgg gtcgcgctcg atgaccacgt cgaggtcctc
     9601 gcgcatcgtg cgcagcagtc cggggacggt gtgcatggct tcaactcctc ctgctacgga
     9661 tggccgtggc atgtggtgtg tccgcgtcgc ccgggtcgtg gccgccgtcc gtgctgggct
     9721 cccgcccggc gccctcgcgc gccgtgggca ggtgcggggg ggcggggcag gtgcggctcc
     9781 ggccggacga agggcgggcg cccgaggggt gcgccgccgg ccggcagcag ccgggtggct
     9841 gtcggggacg gcaccggcag tgcggtgaac aggcccggtg cggccgtttt cgcggccggc
     9901 caggacgtgt ggcgggccgc cgggcagcgg ccgcgcgggc cgtcgggggc cgcgtcggtg
     9961 gacagggcgc cgaaggagac gcggcggcgt gcggtggtgg ttcggcggga cggtgtcagc
    10021 gcgccgccgc cgcgaggacc gcgtcgacca gcttctccag cctgctcacg gtggcggggt
    10081 cctcgatgag gccctcggag gtgcgcaggc tgatcgcgtc gcccacggcg acctcgtcgc
    10141 actccacggc ggcggcaccg cagttggcga gtatccggcg cacgtgcggc tgtacgttcg
    10201 cgccgccggc ctggcccggg gaggcgctca ggaccgccgt cggcaggtcg gtcagcgggc
    10261 tctcgccgcc ggggcgggag agccagtcca gcgcgttctt cagcacgccc ggggggtagc
    10321 cgttgtaggc gggggaacag acgatcaggg cgtcggcacc ggacacctgg ccgcgggcgg
    10381 actccagggg cgcggggtgg cccgcgcgct ccacgtcctg gtcgaacggc gggaagtcgt
    10441 gcagcggcac gacggtggcc tcgtggccac cggcccgccc ctcgatgatc ttccggatcg
    10501 tggcgaccac cgcggagttc gaggactccg cgcgcaggct accggagatc agggcaatcc
    10561 tggccatttc ttcaaccctt tcctagcgtg ctccgcacag actcgcggag ccaatcacgc
    10621 cggggtattc ccggacaaca acgggctcca gggtgcttgc gatttcgggg acttggcgga
    10681 gtgctgtgac agatgtggca agttcctgaa gtcgccgcgg ccgtggccgg atctccttgc
    10741 cacggccgaa ccgcccgtgc ttggcttccc tccatctcag cggcctgccg gtccgcgctc
    10801 cagcgaggca gtgcacgcat atcccggatt tccgccaacg cgggcgtaac gagggatccc
    10861 tgacgccgaa tggcgcggga tgccttgggc ccggcgtgcc aggaaaggaa tgcacggtgt
    10921 cccacttcga tatacgcaag ac
//
