LOCUS       NZ_CP016353            10408 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Prauserella marina strain DSM 45268 chromosome, complete genome.
ACCESSION   NZ_CP016353
VERSION     NZ_CP016353.1
KEYWORDS    RefSeq.
SOURCE      Prauserella marina
  ORGANISM  Prauserella marina
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Prauserella.
REFERENCE   1  (bases 1 to 6851918)
  AUTHORS   Ruckert,C., Albersmeier,A., Jiang,C.-L., Jiang,Y., Kalinowski,J.,
            Winkler,A. and Zotchev,S.B.
  TITLE     Complete genome sequence of Prauserella marina DSM 45268, isolated
            from ocean sediment of the South China Sea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6851918)
  AUTHORS   Ruckert,C., Albersmeier,A., Jiang,C.-L., Jiang,Y., Kalinowski,J.,
            Winkler,A. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2016) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Coverage                   :: 33.7x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002240355.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 02:59:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,595
            CDSs (total)                      :: 6,536
            Genes (coding)                    :: 6,497
            CDSs (with protein)               :: 6,497
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 14 of 39
            Pseudo Genes (incomplete)         :: 31 of 39
            Pseudo Genes (internal stop)      :: 1 of 39
            Pseudo Genes (multiple problems)  :: 6 of 39
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:50:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6159982
            Orig. end                         :: 6170390
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP016353.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10408
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="17"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10408
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10408
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5408](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5408)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    81..95
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 23.44"
                     /tool="antismash"
     gene            846..1529
                     /locus_tag="BAY61_RS28510"
                     /old_locus_tag="BAY61_28330"
     CDS             846..1529
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /locus_tag="BAY61_RS28510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_28330"
                     /product="OmpA family protein"
                     /protein_id="WP_091803226.1"
                     /transl_table=11
                     /translation="MPGNRRLLWLVPLALLVTGVLALLGTWSSSGGIQADLTEKARTAL
                     GSEGLRADVTFDGRDATLHGVAAEQQARAADVVGAVDGVRAVRQAQGAEPSDDGSTDTG
                     AADLNTAIDEELADAPITFRPNTAVLTGKGEQALATVLDTIRSAGDEAKTYGYEVAGYA
                     AKVAGGDPRESARLSSARADTVARKLVDAGISRERVTTVGKGDAPSRGENGDPAKDRRA
                     EITVR"
     gene            1542..2123
                     /locus_tag="BAY61_RS28515"
                     /old_locus_tag="BAY61_28335"
     CDS             1542..2123
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BAY61_RS28515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BAY61_28335"
                     /product="hypothetical protein"
                     /protein_id="WP_091803224.1"
                     /transl_table=11
                     /translation="MWWLFGQIWLWLLIAFVLGAGLTALVLRGGRRTSAATPEGKHERH
                     WERNPTAANETTMIVPPPLVPPTPGEETQFIPTTPSNQDTRHWYEAEEPEHDDYDDYPD
                     YGDFGDYGDFGEDADEAYEEVNGRAPRPEGHTSTAVPLDAVDAGEDEEEAPPPDLGHLA
                     GTLPTRREWHTRNEWPDEQDERERQPHHGG"
     gene            2183..2674
                     /locus_tag="BAY61_RS28520"
                     /old_locus_tag="BAY61_28340"
     CDS             2183..2674
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466521.1"
                     /locus_tag="BAY61_RS28520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_28340"
                     /product="hypothetical protein"
                     /protein_id="WP_091803221.1"
                     /transl_table=11
                     /translation="MPLPHWTSDNVLPQGRHPADLSDLYERFVWDAPHRNEREILFSAL
                     SGYLGVVTRLIPSGRAWVSGPLTDRTPDVPSDVDVVLLPDEWGTLKRLDGPARAALYGM
                     LTLRGVIAEQPAMYLEQVQPVGGLLDGFLCHPGDEEVWADAWSRGAAPGIRKGFAEVTW
                     "
     gene            2668..3555
                     /locus_tag="BAY61_RS28525"
                     /old_locus_tag="BAY61_28345"
     CDS             2668..3555
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669891.1"
                     /locus_tag="BAY61_RS28525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_28345"
                     /product="hypothetical protein"
                     /protein_id="WP_091803899.1"
                     /transl_table=11
                     /translation="MVSFRQLSEGIPGGTWLDDLARASAMAAHAKFERTSRSPLLRVSM
                     LGSTQLDAYTFSDISRALQDATAKIGHIVRNPHGEITYVQPADREKAPLIQRGQAGNAM
                     FFGFPETSAPDALISDGIETLSERAVKELCDFLPANGSDDGALDAVLLQRDTVRNAVSD
                     IVNAVSKNSGIGLSLMPTAGDELTRSMTTEQARVLQGSLRESREDVGYETVTGRLDGVR
                     TRRRIFYLERDTGSTIQGAVAPDLLDEIKLNLDRPVTARLRVVRTTTIDGRRGRPVYEL
                     LEIHSETSLFDQDD"
     gene            complement(4006..4908)
                     /gene="thpD"
                     /locus_tag="BAY61_RS28530"
                     /old_locus_tag="BAY61_28350"
     CDS             complement(4006..4908)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /locus_tag="BAY61_RS28530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_28350"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_091803218.1"
                     /sec_met_domain="PhyH (E-value: 1.2e-32, bitscore: 112.5,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTESRIEDSYPTRVAGEAMLLDRAEPTVWGGEQDGPFDAAELA
                     AHETKGYSIIEGLLSPAEIQGYWQELVRLSSDEELKTDERVITERASGSVRSVFEVHKI
                     SELIGELARDPRILDRASQVLGSDVYLHQSRVNYMPGFKGAGFYWHSDFETWHAEDGMP
                     RPRAVSCSIALTDNYAFNGGLMVMPGSQRTFVQCVGETPDNHYKASLREQKLGVPSEND
                     ITKLAAECGIEQFTGPAGSALFFDSNIMHGSGSNITPYPRSNIFLVFNSVENALVEPFA
                     AAEPRPAFIGARDFTPLKR"
     misc_feature    4899..4913
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.6"
                     /tool="antismash"
     gene            complement(5001..5408)
                     /locus_tag="BAY61_RS28535"
                     /old_locus_tag="BAY61_28355"
     CDS             complement(5001..5408)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947387.1"
                     /locus_tag="BAY61_RS28535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_28355"
                     /product="ectoine synthase"
                     /protein_id="WP_091803215.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.9e-55, bitscore:
                     183.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLDEITDTEADIKTPNWRSKRIILAKERVGFSVHETTLYAG
                     TVNDFWYANHIEAVFIVEGEGEIIDKATGETHELKPGSLYLLNNHDKHQVRPRTDMRTV
                     CVFNPPVTGREVHDENGVYPLVVEDEEPAAS"
     gene            complement(5450..6703)
                     /gene="ectB"
                     /locus_tag="BAY61_RS28540"
                     /old_locus_tag="BAY61_28360"
     CDS             complement(5450..6703)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-357). E-value: 3.2e-72.
                     Score: 235.3. Matches aSDomain:
                     nrpspksdomains_BAY61_RS28540_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156387.1"
                     /locus_tag="BAY61_RS28540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_28360"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_091803213.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7.4e-74, bitscore:
                     247.3, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIFETLESEVRSYSRGWPVVFDRAQGSWLYSEDGKGYLDFFAGA
                     GALNYGHNNPVLKKALIDYISRDGVTHALDMYTVAKRDFLEALSETILKPRSMEYKVIF
                     PGPGGANAVEAALKLARKVTGRESVINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHA
                     TPMPYDRYFDGAYPDFLYFERLLEDSGSGLNEPAAVIVETVQGEGGINAARLEWLQGLS
                     ELCKRHGILLILDDVQMGCGRTGPFFSFEEAGIEPDMICLSKSISGYGLPMAITLIKPE
                     LDVWSPGEHNGTFRGISPAFVTAAEALRTYWSDDALEKSTKAKGERVGSVFKELVETYP
                     SAELVAKGRGLARGLEFTSGEVAGKVCAAAFDRGLLMETSGPDGEVVKLLPSLTTTEAE
                     VDQGLEIISESVKAVLSH"
     aSDomain        complement(5633..6640)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BAY61_RS28540_Aminotran_3.1"
                     /evalue="3.20E-72"
                     /label="BAY61_RS28540_Aminotran_3.1"
                     /locus_tag="BAY61_RS28540"
                     /protein_end="357"
                     /protein_start="21"
                     /score="235.3"
                     /tool="antismash"
                     /translation="FDRAQGSWLYSEDGKGYLDFFAGAGALNYGHNNPVLKKALIDYIS
                     RDGVTHALDMYTVAKRDFLEALSETILKPRSMEYKVIFPGPGGANAVEAALKLARKVTG
                     RESVINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHATPMPYDRYFDGAYPDFLYFER
                     LLEDSGSGLNEPAAVIVETVQGEGGINAARLEWLQGLSELCKRHGILLILDDVQMGCGR
                     TGPFFSFEEAGIEPDMICLSKSISGYGLPMAITLIKPELDVWSPGEHNGTFRGISPAFV
                     TAAEALRTYWSDDALEKSTKAKGERVGSVFKELVETYPSAELVAKGRGLARGLEF"
     gene            complement(6771..7322)
                     /gene="ectA"
                     /locus_tag="BAY61_RS28545"
                     /old_locus_tag="BAY61_28365"
     CDS             complement(6771..7322)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787753.1"
                     /locus_tag="BAY61_RS28545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_28365"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_091803210.1"
                     /transl_table=11
                     /translation="MSGKHSMRANGDNQSADGAGSAGHVVIESPSKADGAALWRIARDS
                     KKLDLNSSYAYVLWCLDFADTSVVARVDGKTVGFVIGYRRPAVADAALVWQVAVDDSQR
                     GRGLAGKMLDALFARLADEGVRYLDTTITPDNEASIRLFESFAKRWEATLERSPLLAAD
                     DFPDTHEREDLFHIGPLKRA"
     misc_feature    7625..7645
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.46"
                     /tool="antismash"
     gene            7688..8944
                     /locus_tag="BAY61_RS28550"
                     /old_locus_tag="BAY61_28370"
     CDS             7688..8944
                     /GO_function="GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /locus_tag="BAY61_RS28550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_28370"
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="WP_143021366.1"
                     /transl_table=11
                     /translation="MAATVTLVTASVAGVIVGTTALGRGTPEAVEATEAARQPNRGHAI
                     VTAHRGSPGLAPEETVASYRQALSEGADVLEGDVQLTADGELVLVHDDTLERTTDVETV
                     FPERAPWRVGQFTLAELKRLDAGSWFHPRFAGERVPTVAELLRTNRGKVGLTLELKAPE
                     NSEGVATKLARELKAAGLADGAVLRSGAYRVMVHSRDEGALKEFHRTAPKVQLAVLTGG
                     RMLDDAELDRLADWTIGVFAHPRVTGAADVERAHSRGLRVISDPVDSPSQIEMASLQGY
                     DWIVTNFPSTARRVTEGKKPFPHANGIVVDHVFPNPSGDDVQPETGEHVTLRNATRKPI
                     DVSGYHLRDQATNLLRIGEGYVLRPGSLLRVYVGPGTNTGDAYYNGLTTGFLNNTGGDT
                     VSVYDPRHKLLDSFSYIVP"
     misc_feature    7699..7714
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.19"
                     /tool="antismash"
     gene            complement(8981..9310)
                     /locus_tag="BAY61_RS28555"
                     /old_locus_tag="BAY61_28375"
     CDS             complement(8981..9310)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1167:
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947796.1"
                     /locus_tag="BAY61_RS28555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_28375"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_091803204.1"
                     /transl_table=11
                     /translation="MKKRSYTCGLDAAIDVMGGKWKGLILFWLGEGPLRFGELRRTVSG
                     ISERVLILQLRELEASGLVHREVHHQVPPKVEYSLTDFGRSLITALAPLGEWGEENMDR
                     LEALP"
     misc_feature    9354..9368
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.95"
                     /tool="antismash"
     gene            9431..10309
                     /locus_tag="BAY61_RS28560"
                     /old_locus_tag="BAY61_28380"
     CDS             9431..10309
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1100: 3-hydroxyisobutyrate dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104110.1"
                     /locus_tag="BAY61_RS28560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_28380"
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="WP_091803201.1"
                     /transl_table=11
                     /translation="MTGQHPVTVIGLGSMGTALAEAFVDAGHPTTVWNRTAAKAAPLVA
                     RGALHARTMNKAITASPLVITCLTSYEDTIDAMGPAAAALEGRALVALNSGSPAAARNM
                     AEWAHGHGARYLDGAVKNVPSAVGAKDTLLYYGGDESVFTEFESTLRVLGGDTVHLGAE
                     VDLAALYEMAVGSTLLPALVGFFQGAAAMQARGLTAASMVRYSAKWFEMISAILPGFAE
                     EIDSGDYGKAASSVNLFHSSIAQDEEFAKEANLDTAWHKPFHDLLERAVEAGHGNHSIS
                     ALTEVLKSRTA"
ORIGIN
        1 attcaacagc ttcgttgcga tcggcgacag cttcaccgag ggtctcaatg acgagttgcc
       61 tgacggcagc ttcaaggggt gggccgaccg gctcgcgggg atcctcgcga acggacgctc
      121 cgagttccgc tatgccaacc tcgcgcttcg cggcaagatg ctcgccgaga tcatgtccga
      181 gcagctgccc atcgcgctgg acatcaggcc cgacctcgtg acggtgtgcg cgggcggcaa
      241 cgacatcatc gttcccggtg ccgacgtcga cgcgacggcg gctgccttcg aggaaggcgt
      301 cgccgcgctc aggtcgaaag gcatcgaggt ggtcatcttc accgggcccg acaccaagag
      361 catgtcggtg atgagcatcc tgcggggcaa agtcggcatc tacaacgccc acctctgggc
      421 gatcgcggag cggcacggcg cgcgggtcgt ggacctgtgg gcgatggacg cgctgcacga
      481 cgtccgcgcg tggagcgacg accggctgca tttcaccccg gaggggcaca ggcggatcgc
      541 gctgcgcacg gccgaggtgc tcggcgttcc caccgagttc gactggcgcg agccgtggcc
      601 ggagtcccct gatcacgcga actggctcac cctgcgccgt tccgacctcg aatggacgag
      661 gatgcacctg cttccctgga ttcgcaggca ccttcgcggc gagtcgatgg gagacggact
      721 ggcgccgaaa cggccgaggc tgacccctct tctcgaccga ccgagtaccg ctgacctggt
      781 ggaacgggcc gacaactagg tcctcatctt tcgcgcgcca tggccggcgc ctttacagtc
      841 ggtgcatgcc gggtaaccgc cgcctgctct ggctggttcc gctcgcactg ctggtgacgg
      901 gcgtgctcgc cctgctcggc acctggtcga gctcgggcgg gatccaagcc gacctcacgg
      961 aaaaggcgag gacggcgctc ggctccgagg ggctgcgggc cgacgtcacc ttcgacggca
     1021 gggacgccac gctgcacggc gtcgcggcag agcaacaagc gcgggccgcc gacgtcgtcg
     1081 gagcggtcga cggggtccgc gccgtgcgac aggcacaggg agcggaaccg agcgacgacg
     1141 gttccaccga cacgggcgcc gccgacctca ataccgcgat cgacgaggaa ctggcggacg
     1201 ccccgatcac cttccggccg aacacggccg tgctgacggg aaagggcgag caggcacttg
     1261 ccaccgtcct cgacacaatc cgctcggcag gagacgaggc caagacctac ggctacgaag
     1321 tggccgggta cgcggcgaag gtcgcgggcg gcgacccacg tgagtcggcg cgcctttcct
     1381 cggcgagggc ggacaccgtc gcgcgaaagc tcgtcgacgc gggaatcagc cgcgaaaggg
     1441 tcaccacggt cggcaagggc gacgcgccgt cgcggggcga gaacggcgac ccggcgaaag
     1501 accggcgcgc tgaaatcacg gtccggtgag ggggtagcga gatgtggtgg ttgttcgggc
     1561 agatctggct ctggctgctg atcgcgttcg tgctcggcgc cggtctgacc gcgctggtac
     1621 tgcgcggcgg caggcggacg agcgcggcca cgccggaagg caagcacgag cggcactggg
     1681 agaggaaccc gacggccgcg aacgagacca cgatgatcgt gccgcccccg ctggtgcccc
     1741 cgacaccggg tgaggaaacc cagttcatcc ccaccacacc gtccaatcag gacacccggc
     1801 actggtacga agctgaggaa cccgaacacg acgattacga cgactacccc gactatgggg
     1861 atttcgggga ctacggggat ttcggggagg acgccgacga ggcatacgag gaagtcaacg
     1921 gccgtgcccc acggccggag ggacacacct cgacggccgt tccgctcgat gccgtcgacg
     1981 cgggtgagga cgaagaggaa gctccaccac cagacctcgg ccacctcgcc ggcacgttgc
     2041 cgacccgacg ggagtggcat acccgcaacg agtggcccga tgagcaggac gagagggagc
     2101 ggcagccgca ccacggtggc tgaggcacgg ctgataagtt cgcctgacca ggtcaaggca
     2161 ggttaggtaa ggaaagctct ggatgccgct cccgcactgg acctcggaca atgtccttcc
     2221 acagggtcga caccccgctg atctctccga cctgtacgaa cggtttgtct gggacgcacc
     2281 acaccgcaac gagcgcgaga ttctgttcag cgccctgagc ggttacctcg gcgtcgtcac
     2341 ccggctcatc ccatcgggaa gggcttgggt cagcggcccg ctcaccgatc gcaccccgga
     2401 tgtcccctcc gacgtcgacg tcgtcttgct gcccgacgaa tggggcacgc tcaagcgact
     2461 cgacggtccc gcgcgcgcgg cgctctacgg gatgctcacc ctgcgcggcg tcatcgcgga
     2521 acaacccgcg atgtatctgg aacaagtgca gccagtcggc ggtctgctcg acggcttcct
     2581 gtgccatccg ggcgacgagg aggtgtgggc cgacgcgtgg tcgcgaggag ccgcacccgg
     2641 gatacgcaaa ggcttcgcgg aggtgacctg gtgagtttcc ggcaactttc ggaaggcatt
     2701 ccgggaggga cctggctcga cgatctcgct cgcgcctcgg cgatggcagc gcacgcgaag
     2761 ttcgagcgca cgtcccgctc cccgctgctg cgggtgtcga tgctcggctc gacccagctc
     2821 gacgcgtaca cgttctccga catcagcagg gcgttgcagg acgcgaccgc gaagatcggc
     2881 cacatcgtgc gcaacccgca cggcgagatc acctacgtgc agcccgccga cagggagaag
     2941 gcaccgctga tccagcgggg ccaggccgga aacgcgatgt tcttcgggtt tcctgagacc
     3001 tccgcgccgg acgcgctcat ctcggacggc atcgaaaccc tctccgaacg cgcggtgaag
     3061 gagctgtgcg acttccttcc ggccaacggc tccgacgacg gtgcgctcga cgccgtgctc
     3121 ttgcagcggg acaccgtccg caacgccgtc agcgacatcg tcaacgcggt cagcaagaac
     3181 tccggcatcg ggctgtccct catgccgaca gccggcgacg agctcacccg cagcatgacg
     3241 acggagcagg ccagggtttt gcagggcagc ctgcgagagt cgagggaaga cgtcggatac
     3301 gagacggtca ccggaaggct cgacggtgtc cgcacgcggc gcaggatctt ctaccttgag
     3361 cgcgacaccg gcagcaccat ccagggcgcg gtcgctcccg acctgctcga cgagatcaag
     3421 ctgaacctcg accgtccggt cacggcacgc ctgcgggtcg tccgcaccac gaccatcgac
     3481 ggcaggaggg ggaggccggt gtacgagctg ctggagatcc actccgagac gagcctcttc
     3541 gaccaggacg actgagcttc caaggcaagc ccagacgagt acggccccgt gccatcagtc
     3601 ccgatctgat ggcgcggggc cgtactcggt tcagccgttc gtcctgacca acgtggtgtt
     3661 agagcccacc ccgagcgaag gagcaacccg cgccacgctg gtcacgacta cctcatccgt
     3721 aacgtcaatg aggtggcgca cacagtagca ggctgtgtga gggccgctac aaccaccccc
     3781 acacagccgg cgactgatca cttagtgtgc gttttcttaa gtggcggagc cgcttggagc
     3841 gggcaagcaa ccctctccgc agcgggttct gaggtggatc ctcgcgagga cagcgacgac
     3901 gtggtcggaa tacatgcgga gacgatcccg cagcgaggag ccacctcagg ttccgccacc
     3961 cgcaccgcca cccaaaccac ttccgaaaca gtccctacgc gggggtcagc gcttcagcgg
     4021 ggtgaagtcc cgggctccga tgaaggcggg ccggggctca gccgccgcga acggctcgac
     4081 cagggcgttc tcgacgctgt tgaacacgag gaagatgttc gacctcgggt agggcgtgat
     4141 gttgctgccc gacccgtgca tgatgttgga atcgaagaac agcgcggacc ccgcaggtcc
     4201 ggtgaactgt tcgatcccgc actcggcggc gagcttcgtg atgtcgttct cgctcggcac
     4261 cccgagcttc tgctccctca gcgaagcctt gtagtggttg tccggagtct cgccgacgca
     4321 ctgcacgaac gtgcgctgcg aacccggcat gaccatcagg ccgccgttga acgcgtagtt
     4381 gtcggtcaag gcgatcgagc agctcacggc tctcgggcgg ggcatcccgt cttcggcatg
     4441 ccacgtctcg aagtcggagt gccagtagaa acccgcacct ttgaagccgg gcatgtagtt
     4501 gaccctgctc tggtggaggt agacatccga gccgaggacc tggctcgccc tgtccaggat
     4561 gcgaggatcg cgcgcaagct cgccgatcaa ctcgctgatc ttgtgtactt cgaagacgga
     4621 ccggacactg cccgatgctc gctcggtgat cacgcgctcg tcggttttga gttcctcatc
     4681 cgacgagagc cgtacgagct cctgccaata cccctgaatt tcggcggggg acagcagtcc
     4741 ttcgatgatc gagtacccct tggtctcgtg cgccgccagt tcggccgcgt cgaacgggcc
     4801 gtcctgctca ccgccccaca ccgtcggttc cgccctgtcg agaagcatcg cttcaccggc
     4861 gacacgggtc ggatagctgt cctcaatccg gctctctgtg agagtcactt ttactcgcct
     4921 cctacgagcc actcttttgt ttgtcgtatc ccttcctgtg ggaaaacggg gccgggctgg
     4981 cgtgcccggc cccgttcaat tcacgaagcc gcgggctctt cgtcctcgac gaccaagggg
     5041 tataccccgt tctcgtcgtg aacctcgcgt ccggtgaccg gcgggttgaa cacacacacg
     5101 gtccgcatgt cggttcgcgg gcgaacctgg tgcttgtcgt ggttgttcag cagatacagc
     5161 gaaccgggct tgagctcgtg cgtctcaccg gtcgccttgt cgatgatctc gccttcacct
     5221 tcgacgatga aaaccgcctc gatgtggttg gcgtaccaaa agtcgttgac ggtacccgcg
     5281 tacagcgtgg tctcgtgcac cgagaagccg acccgctctt tggcgagaat gattcgctta
     5341 ctgcgccagt tgggcgtctt gatgtcggct tccgtgtcgg tgatctcgtc gagactgcgg
     5401 acgatcaaag ctggtttccc ttctgctttt gtgtgtgttg cgaatggact cagtgggaga
     5461 gtacggcctt gaccgattcg gagatgatct ccagtccttg atcgacttcg gcttccgtcg
     5521 tcgtcagcga gggaagcagc ttgacgacct ctccgtcggg tcccgacgtc tccatcaaca
     5581 ggccacgatc gaaagcagcc gcacacacct tgcccgcgac ctcgccgctg gtgaactcca
     5641 gtcccctcgc cagtcccctg cccttggcga ccagctctgc ggagggatac gtctcgacga
     5701 gctccttgaa gacgctcccg acgcgctcgc ccttggcctt cgtcgacttc tccagcgcgt
     5761 cgtcgctcca gtaggtacgc agcgcttccg cggcggtcac gaacgcgggg ctgatcccgc
     5821 ggaaggtgcc gttgtgctcg ccgggcgacc acacgtcgag ctcgggcttg atcagcgtga
     5881 tcgccatcgg aaggccatag ccactgatcg acttcgaaag gcagatcatg tccggctcga
     5941 tgccggcttc ctcgaagctg aagaacggcc ccgtcctccc acagcccatc tggacgtcgt
     6001 cgagaatgag caggatgccg tgccgcttgc acagctcgga aaggccctgc aaccactcca
     6061 gcctcgccgc gttgatgccg ccctcaccct gaacggtctc cacgatgacc gcggcaggct
     6121 cgttcagacc gcttcccgag tcctcaagca atcgctcgaa gtacaggaag tcggggtagg
     6181 cgccgtcgaa gtagcggtcg tacggcatcg gggtcgcgtg cacgagcgga ataccggcgc
     6241 cgccccgctt catcgagttc ccggtcaccg agagcgcgcc gagggtcatg ccgtggaacg
     6301 cgttggtgaa gttgatgacc gactccctgc cggtcacctt gcgagcgagc ttgagcgccg
     6361 cctcgaccgc gttcgcgcca ccggggccag ggaaaatgac cttgtattcc atggagcgcg
     6421 gcttgagaat cgtctcgctg agcgcttcga ggaaatcgcg cttcgcgacg gtgtacatgt
     6481 cgagcgcgtg agtgacgccg tcacgggaaa tgtaatcgat cagcgccttt ttcagcaccg
     6541 gattgttgtg gccatagttg agcgcaccgg caccggcgaa gaagtcaagg taacctttac
     6601 cgtcttccga gtagagccaa ctgccttgcg ctcgatcgaa caccacgggc cagcctcggc
     6661 tgtagctccg cacttccgat tcgagcgttt cgaagatgct caccttgctc cgtctcctgc
     6721 tttatagggc gaattgtcgc tcatcgcgtt ggaacttatc gcgctgacta tcaggcacgc
     6781 ttgagtggac cgatatgaaa taggtcttcc cgctcatgcg tatccggaaa atcgtcggcc
     6841 gcaagtagcg gcgagcgttc caacgtggct tcccagcgct tggcgaatga ctcgaacaac
     6901 cggatcgaag cctcgttgtc gggcgtgatc gtcgtgtcga ggtaccgcac tccctcgtcc
     6961 gcgaggcgcg cgaaaagggc atcgagcatc ttgcccgcga gccctcgtcc tcgctgactg
     7021 tcatcgacgg cgacctgcca taccagcgcc gcgtcggcga cagcgggcct gcggtagccg
     7081 atgacgaagc ccacagtttt tccgtcgact ctcgcgacaa ccgaggtgtc cgcgaaatca
     7141 agacaccaca gcacgtaagc gtaagacgag ttgagatcca gctttttcga atcacgtgct
     7201 attcgccata gcgcggcgcc gtctgctttt gaaggcgatt cgatcacgac gtgaccagca
     7261 gagccagcac catccgccga ttgattatcg ccgttggcac gcattgagtg ctttccggac
     7321 atattcaagc aacgtaacag agtgcgttac tcgattcagc ccagcgccgt cacagcatgg
     7381 cgacctacct gcaatgactc agaaacacct gtgagaatgc cgacagaaag gtaccgatca
     7441 tgccctgacc tgtgataatg aagcgcagaa cagatcgccg ctgactaatc cagcgcccgc
     7501 ttgcacgtta gtggcagcgg acttcatacg cgagagggca gtcggacgaa cactgtgtgt
     7561 cagcgtccgt ttggtgagga tttcactgaa tgtttccgcg ttgccatcac acctcggcac
     7621 gatcgtcggg tcacattgac gaacactggg agtcgaagtg ggtcaacgtt cgcgcgccct
     7681 tggcagggtg gcggcaactg taacactcgt cacggcgtcc gtcgcggggg tgatcgtcgg
     7741 aacgaccgcg ctcggccgcg gtactcccga ggccgtcgaa gcaaccgaag ccgcacggca
     7801 accgaaccgg ggccacgcca tcgtcaccgc gcacagaggc tcaccaggtc tggcgccgga
     7861 ggagaccgtc gcgagttacc ggcaggcact gtcggaaggc gccgatgtcc tcgaaggaga
     7921 cgtccagctc acggctgacg gcgaactggt cctcgttcac gacgacactc tggaacgtac
     7981 gaccgacgtc gaaaccgtct ttcccgaacg cgcaccgtgg cgagtcggcc agttcaccct
     8041 cgccgagctc aaacgactcg acgccggatc gtggttccac cccaggttcg ccggagaacg
     8101 cgttcccacc gtcgccgaac tgctgcggac caacagggga aaggtagggc tcacccttga
     8161 gctgaaggcg ccggagaaca gcgagggcgt cgcgacgaaa ctcgcgaggg aactgaaggc
     8221 ggccggactc gccgacggtg ccgtgctgcg ttccggcgcc taccgcgtca tggtgcactc
     8281 gcgcgacgag ggagcgctca aggaattcca ccggaccgcg cccaaggtgc agctcgcggt
     8341 gttgaccggg ggcaggatgc tcgacgacgc cgagctcgac cggctggcgg actggacgat
     8401 cggggtcttc gcgcatccga gggtcaccgg cgccgccgac gtcgaaaggg cacactcgcg
     8461 tggcctgcgc gtgatcagtg accccgtcga cagtccatcc cagatcgaga tggcctccct
     8521 acagggctac gactggatcg tgacgaactt cccctcgacc gcacgaaggg tcaccgaggg
     8581 caagaagccc ttcccccacg cgaacggcat cgtcgtggac cacgtgttcc ccaatccgag
     8641 cggtgacgac gtgcagccgg aaaccggcga gcacgtgacc ctgcgcaacg ccacccgcaa
     8701 gccgatcgac gtcagcggct accaccttcg cgaccaggcg acgaatctgc tgcgcatcgg
     8761 tgagggatac gtgctgaggc caggcagcct gctgcgggtg tacgtcgggc cgggaaccaa
     8821 caccggcgac gcctactaca acgggttgac gacgggattc ctcaacaaca ccggcggcga
     8881 caccgtttcc gtgtacgacc cgcggcacaa gctgctcgac agcttctcct acatcgtgcc
     8941 ctgagacgcc tgatggtggg accggcaccg gtcccaccac tcacggaagt gcctcaagcc
     9001 ggtccatgtt ctcctcgccc cactcaccga gcggagcgag tgcggtgatg agcgagcgac
     9061 cgaagtcggt cagcgaatac tccaccttcg gcggcacctg atgatggacc tcgcggtgca
     9121 ccaggccgct tgcctccagc tcccggagtt gcaggatcag cacccgctcg ctgatcccgg
     9181 acaccgtccg gcgcaactcc ccgaatcgca gcggtccttc gccgagccag aagaggatca
     9241 gccccttcca cttgcctccc atgacgtcga tcgcggcgtc gagcccgcag gtgtagctcc
     9301 gcttcttcat cgcgctccct gacaaaaatg taagtacccg acaaaattgt cggtacttgc
     9361 agaaaagttt gctccctcgc aggctgaaaa ggcaaccgac gatctcccgc tttcgaacag
     9421 gggcaaacgt atgaccgggc aacaccccgt gacggtgatc ggactcggct cgatgggcac
     9481 ggcactcgcc gaggcattcg tcgatgccgg acacccgacc accgtctgga acagaaccgc
     9541 cgcgaaggcc gcgccgctgg tggccagagg cgctcttcac gcgcgcacga tgaacaaggc
     9601 gatcacggcg agcccgctgg tcatcacctg cctgacctcg tacgaggaca ccatcgacgc
     9661 catggggccc gcggctgccg cgctcgaagg gcgcgcgctg gtggcgctca acagcggctc
     9721 accggccgcg gcgaggaaca tggccgaatg ggcgcacgga cacggcgcgc gctacctcga
     9781 cggggcggtc aagaacgtgc catccgcggt tggcgccaaa gacaccctgc tctactacgg
     9841 cggcgacgag agtgtcttca ccgagttcga atcgacgctg cgagtgctcg gtggcgacac
     9901 cgtccacctc ggcgcggagg tggacctcgc cgcgctgtac gagatggcgg tgggaagcac
     9961 gctgctacca gcgctcgtcg gcttcttcca gggtgccgcc gccatgcaag cccgcggcct
    10021 cacggcggct tcgatggtca ggtacagcgc gaagtggttc gagatgatca gtgcgatcct
    10081 tcccggattc gccgaggaga tcgacagcgg tgactacggc aaagccgcct cctccgtgaa
    10141 cctcttccac tcctccatcg cccaggacga ggagttcgcg aaggaggcaa atctcgacac
    10201 ggcctggcac aagccttttc acgacctcct cgaacgcgcc gtcgaagcag gtcacggcaa
    10261 ccacagcatc tccgcgttga cggaagttct caaaagccgg acggcctagc gcttccgaaa
    10321 attcaccttg acatcaagtg aagtcgatgt tcgaaggtgg gtgtcatgag caccgacatc
    10381 gaaacgcagt ccacccaagg cgacatcg
//
