LOCUS       NZ_CP016353            10384 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Prauserella marina strain DSM 45268 chromosome, complete genome.
ACCESSION   NZ_CP016353
VERSION     NZ_CP016353.1
KEYWORDS    RefSeq.
SOURCE      Prauserella marina
  ORGANISM  Prauserella marina
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Prauserella.
REFERENCE   1  (bases 1 to 6851918)
  AUTHORS   Ruckert,C., Albersmeier,A., Jiang,C.-L., Jiang,Y., Kalinowski,J.,
            Winkler,A. and Zotchev,S.B.
  TITLE     Complete genome sequence of Prauserella marina DSM 45268, isolated
            from ocean sediment of the South China Sea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6851918)
  AUTHORS   Ruckert,C., Albersmeier,A., Jiang,C.-L., Jiang,Y., Kalinowski,J.,
            Winkler,A. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2016) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Coverage                   :: 33.7x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002240355.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 02:59:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,595
            CDSs (total)                      :: 6,536
            Genes (coding)                    :: 6,497
            CDSs (with protein)               :: 6,497
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 14 of 39
            Pseudo Genes (incomplete)         :: 31 of 39
            Pseudo Genes (internal stop)      :: 1 of 39
            Pseudo Genes (multiple problems)  :: 6 of 39
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:50:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3789508
            Orig. end                         :: 3799892
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP016353.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10384
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="10"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10384
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10384
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5384](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5384)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    600..614
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.37"
                     /tool="antismash"
     misc_feature    942..954
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.75"
                     /tool="antismash"
     misc_feature    1028..1040
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     gene            1076..1477
                     /locus_tag="BAY61_RS17775"
                     /old_locus_tag="BAY61_17695"
     CDS             1076..1477
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BAY61_RS17775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BAY61_17695"
                     /product="hypothetical protein"
                     /protein_id="WP_143021421.1"
                     /transl_table=11
                     /translation="MPISSASTREAMLSGVAEAGDQAIRRGTAGDAENTPALRELYDQL
                     AGTMCAVMGSTDVVEELGRLRSALTAAAPALRAVSPDVLDTIQEGLAQPDPRVLMSALI
                     AARRLLCRVLTPQRWGNRLTAAEQILWAR"
     gene            1669..2961
                     /locus_tag="BAY61_RS17780"
                     /old_locus_tag="BAY61_17700"
     CDS             1669..2961
                     /GO_function="GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458428.1"
                     /locus_tag="BAY61_RS17780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_17700"
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="WP_091808687.1"
                     /transl_table=11
                     /translation="MPAPRRSWLAVVTGASVLALVAATTPAVAQPAPQAAPATEQCSDE
                     PRDLPVHEFTDHRELGVELARIERVSDGAVDVTEVGRSNRGKEVWSARVGTGPKAVLIT
                     SEIHGNEKTGTDAILQLLDWVGTSDTAKAKRWRSELTIVAVPKMNPDGAELDRRGNDMT
                     WDEVTARFPQLRGSEPAWNYYTEPRQGDDYSQLPGFDVNRDYNPDLSYEPKAEDFPGSS
                     ADTGWYITPESRTVRDVYKGLVAEFGKVDTYVDLHHQGACYVMPDDPSRFVTMSISGKF
                     VDDPATMPGYEQYAEDYDLDYSKRLNVAIYNALQGRANGNPLFGNITLYPQDTNLPGTG
                     LGSFALNGSGAVLFEVRGQTQTLGQKYRAQLTRTVYIGLEGMLSSLASGQVDHLDPAVY
                     DTIPPRGPSIGSSGTDTRTAAAEANPEVLEN"
     gene            complement(3019..3495)
                     /locus_tag="BAY61_RS17785"
                     /old_locus_tag="BAY61_17705"
     CDS             complement(3019..3495)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005456847.1"
                     /locus_tag="BAY61_RS17785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_17705"
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="WP_091808689.1"
                     /transl_table=11
                     /translation="MKLPESTEWALHCTTALAQLGGEPVSTSQLAEHFGLPVPYLAKQL
                     AHLVKAGVLSGTTGPRGGFRLALPATRITVLDIVEAIDGRGDPYQCREIRQQGRGAVPA
                     ADCLEPCVLATTMRAAHEAWRASLAAVTVAELVGTLPPEIPQRTRRLLSRGGGE"
     gene            3564..4322
                     /locus_tag="BAY61_RS17790"
                     /old_locus_tag="BAY61_17710"
     CDS             3564..4322
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005438426.1"
                     /locus_tag="BAY61_RS17790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_17710"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_091808691.1"
                     /transl_table=11
                     /translation="MRVAVIGATGRIGALTTEALVAAGHDVVPVSRSHGIDVREGTGLR
                     SALDGAEAVIDTTNSTATDEAETVGFFTTTTRNLLAAEREAGVRHHVVLSIAGVGNVGG
                     NAHYAGKRAQEAEVASGGVPCTIVPATQFHDFAAMVASWTEADGVARIAPLLVQPIAPV
                     DVAAILARVATGAPQGRHADVAGPGTQDLVDMARRTYAARGRSITLVPTWHTGIFDVTM
                     AGDVLLPAPGAEIAPTSFDTWLDDEKRARR"
     gene            complement(4344..4961)
                     /locus_tag="BAY61_RS17795"
                     /old_locus_tag="BAY61_17715"
     CDS             complement(4344..4961)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BAY61_RS17795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BAY61_17715"
                     /product="disulfide bond formation protein DsbA"
                     /protein_id="WP_091808693.1"
                     /transl_table=11
                     /translation="MTTSTAGDSPRWAADFWLDPACPLTRVTARWLVTVADEIPLDIRW
                     RVMSLSILNEGKDVDPEGDEDGFLWIPARIAAAVQTGHGHAALGEFHTALWTDPDGTDR
                     EWFGDIADALRRCDLPESLVESGFSTGYDAELRASHEEGVSRIQAEVGTPILSITTPEG
                     ARHTAFGPVITAVPDGTGALDLWHATLRLAALPEFRELKIGR"
     gene            complement(5001..5384)
                     /locus_tag="BAY61_RS17800"
                     /old_locus_tag="BAY61_17720"
     CDS             complement(5001..5384)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF009806.0"
                     /locus_tag="BAY61_RS17800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_17720"
                     /product="ectoine synthase"
                     /protein_id="WP_091808694.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.1e-41, bitscore:
                     139.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYVTSLADTIGTAGDVHGPGWRSIRLLLAGAGLGYSFHDSTIYAG
                     TELLIQYAHHSESVFCVEGQGVVVDLGSGRSHDVSPGTMYVLDGHDRHLLRAVTDLRII
                     CVFTPALVGAESHDENGAYPAAG"
     gene            complement(5423..6520)
                     /locus_tag="BAY61_RS17805"
                     /old_locus_tag="BAY61_17725"
     CDS             complement(5423..6520)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /locus_tag="BAY61_RS17805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_17725"
                     /product="aspartate kinase"
                     /protein_id="WP_170140266.1"
                     /transl_table=11
                     /translation="MNPGSAVSVLKFGGSTFATPSRYREVAAMIRRRAAESGEGQVVVV
                     SAMPGDTERLRQRVHAACAVPGQNALVSLLPMADTMSALLLAAALDAEGRVAEVLTGQA
                     TGFTTRGSPLWATLSGIDPAPLRDALRRGSCVVLPGGQAGDESGRPTWLGKNSSDLSAV
                     AAAVATGAAHCEIFSDVDGVYSCDPRVVEESRLLGEVAYPLAQRLTRYGAKVLHPEAVR
                     LAARHGLRIVCAANTPPYRRGSVIGGIDSAEEPGAVVLNRLSRAFTSPSVSSAEETAAQ
                     LAAEGINALAAHTRKGPVVLVIGGYVDLGEVCERRGVALGGPAGIPVVGIEGERVEVHL
                     ADDEAAGVALARSLHGRLLPSLAPS"
     gene            complement(6517..7566)
                     /locus_tag="BAY61_RS17810"
                     /old_locus_tag="BAY61_17730"
     CDS             complement(6517..7566)
                     /EC_number="1.2.1.11"
                     /GO_function="GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA]"
                     /GO_function="GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /GO_function="GO:0051287 - NAD binding [Evidence IEA]"
                     /GO_process="GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /locus_tag="BAY61_RS17810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_17730"
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="WP_091808698.1"
                     /transl_table=11
                     /translation="MASDRPQHASDAPRVAVVGATGAVGMSLLQLVEERGFDYASFELV
                     ASPRSAGRTVEFGGAELVVREIDDVDFGSVDVAFFSAGTALSRIWAPRASAEGALVIDN
                     TAAFRMDPDTPLVVPQVNASVLDRRPASGIVANPNCSTIPLVRVLRPVLREWGAEQVVV
                     STYQAASGGGYSGIGELREDARSQLAGPPYSVPVKEFPPGLAFNVIPFIDTVLDDGGTV
                     EERKMAQESAKILASPELDVTATCVRVPVVNGHSEAVWIRTRRPAEREAVIAELAAQPD
                     VVVHESTDPVSTPTPRHNGDPDRVHVGRIRVSEDGCGVWLWLVADNLRIGAALNAIQIA
                     ELLREKGKL"
     gene            complement(7570..8817)
                     /locus_tag="BAY61_RS17815"
                     /old_locus_tag="BAY61_17735"
     CDS             complement(7570..8817)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647139.1"
                     /locus_tag="BAY61_RS17815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_17735"
                     /product="methylaspartate mutase"
                     /protein_id="WP_245865177.1"
                     /transl_table=11
                     /translation="MNSPPERSPVPVERDGLVVQCRMGFSDIDLMRRGLRAVRDSGLPT
                     VGTITLDSFTRTNQHARARSALAEGRTLNGYPIVAHGARRTRELLADVAGPGFGIQMRH
                     GSARPFEIFEVMLDAGITVTEGGPVSYCLPYSRVPLDVATEEWERSCALLGAYQGHVET
                     FGGCLLGQLCPPDLLVAISVLEALFFQHNGIRDVSLSYAQQVHFGQDLEALLALRELAS
                     SYLAADVGWHVVVYTYMGVYPASRRGALGLLAESARLAAAAGCERVIVKTVAEATRIPT
                     IEENVAALRHAHDAALAQRDRPPAPAGTGIEERARALIDTVLGLADTVGGALVRAFRGG
                     VLDVPYCLHPDNANVARSGIDERGRLIWADPGAMPISHLVPRARAQRVTSAELLRMLAH
                     VRRRFDTDAPATAEGV"
     gene            complement(8814..9287)
                     /locus_tag="BAY61_RS17820"
                     /old_locus_tag="BAY61_17740"
     CDS             complement(8814..9287)
                     /GO_function="GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647138.1"
                     /locus_tag="BAY61_RS17820"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on a
                     cobalamin-like analog.; Presence of a B(12)
                     (cobalamin)-binding domain implies dependence on cobalamin
                     itself, in one of its several forms, or in some unusual
                     lineages, dependence on a cobalamin-like analog.; Presence
                     of a B(12) (cobalamin)-binding domain implies dependence on
                     cobalamin itself, in one of its several forms, or in some
                     unusual lineages, dependence on a cobalamin-like analog.;
                     Presence of a B(12) (cobalamin)-binding domain implies
                     dependence on cobalamin itself, in one of its several
                     forms, or in some unusual lineages, dependence on a
                     cobalamin-like analog; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_17740"
                     /product="cobalamin-dependent protein"
                     /protein_id="WP_211323619.1"
                     /transl_table=11
                     /translation="MPEKRIVPAPVAVVATTPSDAHTWNLVYLQLLLEEQGFVVHNLGP
                     CVPFREVVGECVATAPDLLVLSSVNGHGAIEIPACAAAVRARPEVAGMRIVVGGKLTTE
                     GFLENRKNREMLSSGVDAVFSPEQPVGDFIDYLKQFSGVAERLPTTRRGSGIR"
     misc_feature    complement(9414..9428)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.39"
                     /tool="antismash"
     misc_feature    9415..9428
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.87"
                     /tool="antismash"
     gene            9535..10323
                     /locus_tag="BAY61_RS17825"
                     /old_locus_tag="BAY61_17745"
     CDS             9535..10323
                     /GO_function="GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1228: methionyl-tRNA formyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /locus_tag="BAY61_RS17825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_17745"
                     /product="formyltransferase family protein"
                     /protein_id="WP_170140267.1"
                     /transl_table=11
                     /translation="MNQRLSESPVARPFRIPGADRPGDLRFAYFNLKGHPRGELMLHRL
                     IGAGFVPSLVIDEDSPLAAGLLAAQFRQLGQVAAFTPPTPLETVCKTLDIRYETVGNHN
                     DADTVAVLAEAAPSLGVLGDTRIMRARVIDALPHGIVNVHPGLLPSVRGNNPYLWSVVH
                     GLPQGATAHLIDTGVDRGPIMLARELELTAGTTLPELIHTINTLCADVVVTALSQLTSG
                     TATLTEQPADDAVTFREARPEIWSLVETILRERAGAAARR"
ORIGIN
        1 cctcgccgcc gtggcaccgg acctgcgagg ccccgcgttg ttgtggctac ccttcgaact
       61 gaggtcacgc accctgctgc gactgctgcg gctggctccg gcaccggcga cgccgctggt
      121 tcccggggtg agcgtgtccc ggcgagacac cggcgacggc gcggaaagcg gcacaggtat
      181 ccacgtctac gaaccggccg accggactcg cccctccggc gcgctgctct ggttgcacgg
      241 cgggggctac gtcatgggat ctccggtcag cgaccacacg ctgtgcggcc ggttcgcggc
      301 ggaactggga atcctcgtcg tgagcgtcga ctaccgcctc gctcccgaac accccttccc
      361 cgaagggctc gacgacgccg tcaccgtgct ccggtggtcg cgccgccacg cgggcgagct
      421 gggtatcgac ccggagcggg tcgccgtcgg aggtgaaagc gcgggtggcg gcctcgccgc
      481 ggcgcttgcc cagcgagcac acgacgaggg cgatctttcg ccgtgcttcc agttgctggt
      541 ctatccgatg ctcgacgacc ggaccgcgct cgacgccgcg cgtgagccca ccttggtctg
      601 gtccccctcg gccaaccggt tcggctggag ctcctacctc ggccacccgc tttcggctgg
      661 ggagacccgc ccctacgcgg ttcccgcgag gcgcgcggat ctcggcggtc tcgcaccggc
      721 gtggatcggg gtcggcgatc tcgatctctt ccacgacgaa gacgtcgact acgcccaccg
      781 gctcggtgag gccggggtgg actgcctgct ggaggtcgtg ccggggatgt accacggcgc
      841 gttgtcgctg gtgcccggcg cgccctcggt cacggcgttt cgggccagcg cgatccgtgc
      901 actgggcgcg gcggtgaacc cgccaccgcc cgtcagccaa tcagcaattt gcaagacatg
      961 aacccgattc caatgcgaac atggcctgga cgtcggggtt ctcgtggtca cacaatcagg
     1021 tggtagtttg caatttgcgg tgtgaaccgc gcctggcaac cgttagacaa gctgggtgcc
     1081 gatcagttcc gcgtcaacca gggaggccat gttgtccggt gtcgccgaag ccggggatca
     1141 ggccatccgc cgcggtacgg ccggcgacgc ggagaacacc cccgcgctac gcgagctcta
     1201 cgatcagctc gcgggcacga tgtgcgccgt catgggctcc acggacgtcg tcgaagagct
     1261 gggcaggctc cgcagcgcgc tcacggccgc cgcgccggca ttgcgcgccg tctcccccga
     1321 cgtgctcgac acgatccagg aggggctcgc ccagcccgac cccagagtgc tgatgagcgc
     1381 gctgatcgcc gcacggcgcc tgctgtgccg ggtactgacc ccgcagcgct ggggaaaccg
     1441 gctcaccgcc gcggagcaga tcctgtgggc acggtgaggc gttccggttc cgcgcgcaac
     1501 gccggacgtt gtgggccggg cacgtggaat tcggccggat tcggcgctgg ggacttttca
     1561 ccgaactagt tagccgctat aactaacgat cacggtggcg ccacctcgac accacagcca
     1621 gaaacccgtg ccacggcacg ggttcttcgc agagtccgag gagttgccgt gcccgcacca
     1681 cgacgttcgt ggctggccgt cgtcaccggg gcgagcgtgc tcgcactggt ggccgcgacc
     1741 accccggccg tggctcagcc cgccccgcaa gcggcccccg ccacggagca atgcagtgac
     1801 gagccacggg acctgcccgt tcacgagttc accgaccacc gcgaactcgg cgtcgaactc
     1861 gcccgcatcg agcgggtcag cgacggcgcc gtcgacgtga ccgaggtagg cagaagcaac
     1921 aggggtaagg aagtctggtc ggcccgcgtc gggaccggcc ccaaggccgt gctgatcacc
     1981 agcgagatcc acggcaacga gaagaccggc accgacgcga tcctccagct gctcgactgg
     2041 gtcggaacca gtgacaccgc gaaggcgaag cggtggcgct ccgagctgac gatcgtggcc
     2101 gtcccgaaga tgaaccccga cggcgccgaa ctcgaccggc gtggcaacga catgacgtgg
     2161 gacgaggtga ccgccaggtt cccgcagcta cgcggaagcg aaccggcgtg gaactactac
     2221 accgaacccc ggcagggcga cgattactcg caactacccg gattcgacgt caacagggac
     2281 tacaaccccg atctgtccta tgagcccaag gccgaggact tccccggttc ctccgcggac
     2341 accggctggt acatcacgcc ggaatcgcgc acggtccgcg acgtgtacaa gggactggtc
     2401 gccgagttcg gcaaggtcga cacctacgtc gacctgcacc accagggcgc ctgctatgtg
     2461 atgccggacg acccgagccg gttcgtgacc atgtccatct ccggcaagtt cgtcgacgac
     2521 ccggccacga tgccgggata cgagcagtac gccgaggact acgacctcga ctactccaag
     2581 cggctcaacg tcgcgatcta caacgcgttg cagggaaggg ccaacggcaa cccgctgttc
     2641 ggcaacatca ccctgtatcc ccaggacacc aacctgccgg gaaccggcct cggttccttc
     2701 gcgctcaacg gcagcggcgc ggtgctgttc gaggtacgcg gccagaccca gacgctgggc
     2761 cagaagtacc gcgcccagct gaccaggacc gtctacatcg gattggaagg gatgctgtcc
     2821 tcgctcgcca gcggtcaggt ggatcacctc gatcccgccg tctacgacac catcccacca
     2881 cggggtccca gcatcggcag cagcggcacg gacacccgca ccgcggcagc cgaggcgaat
     2941 ccggaagtgc tggaaaacta accgcaccga cagcgaaggc gggggtggcc accgatgtgg
     3001 ccacccccgc ctcgcctgtc attccccgcc gccgcgcgac agcaaccggc gggttcgctg
     3061 gggaatctcc ggtggaagcg tgccgacgag ttccgcgacg gtgaccgccg ccagactcgc
     3121 ccgccatgcc tcgtgcgccg cgcgcatcgt cgtggccagc acgcagggtt ccaggcagtc
     3181 ggccgcgggt accgctccgc gtccctgctg ccggatctcc ctgcactgat agggatcgcc
     3241 gcgcccgtcg atggcctcga cgatgtcgag gacggtgatc cgcgtggcgg gcagcgcgag
     3301 ccggaaaccg ccccggggtc ccgtcgttcc cgagagcacc cctgccttga ccaggtgagc
     3361 gagctgcttg gccagatagg gcaccggaag accgaagtgt tcggcgagct ggctcgtgct
     3421 gacgggctcg ccgccgagtt gcgcgagcgc ggtggtgcag tgcagggccc attcggtgct
     3481 ctcgggcagc ttcataccct cagcatatag ctgctattgt ggatccgtta agtcggcaat
     3541 agcgcgggag cagaggagct gtcatgcgag tcgcggtcat cggagccacc ggacggatcg
     3601 gagcgctcac caccgaagcc ctcgtcgcgg cgggacacga cgtcgtgccg gtcagcagaa
     3661 gtcatggcat cgacgtgcgt gagggaacag gcctccgctc cgcgctcgac ggcgcggagg
     3721 cggtgatcga cacgaccaac agcaccgcga ccgacgaggc cgaaaccgtc gggttcttca
     3781 ccacgacgac acggaacctg cttgccgcgg aacgcgaagc cggggtaagg caccacgtgg
     3841 tgctgtcgat cgccggtgtc ggcaacgtcg gcgggaacgc gcactacgcg ggcaaacgcg
     3901 ctcaggaggc cgaggtcgcc tcgggcggcg ttccctgcac gatcgttccc gcgacccagt
     3961 tccacgactt cgcggcgatg gtcgcgtcgt ggaccgaggc cgatggcgtc gcgcggatcg
     4021 cgccattgct ggtgcagccg atcgctccag tcgacgtcgc cgcaattctg gccagggtcg
     4081 ccaccggcgc gccccaggga cggcatgccg acgtcgcggg tcccggtaca caggacctgg
     4141 tggacatggc acggcgcacc tacgcggcgc gcggccggtc catcacactg gtgccgacct
     4201 ggcacaccgg aatcttcgac gtcacgatgg ccggggacgt acttcttccc gcgcccggcg
     4261 cggagatcgc gccgacctca ttcgacacct ggctggacga cgagaagcgc gcccggcggt
     4321 gacggtatgt ccacaacgga ctctcagcgg ccgatcttga gctcgcgaaa ttccggcagc
     4381 gcggcgagcc tgagcgtcgc gtgccacagg tcgagcgcac cggtcccgtc cggtactgcc
     4441 gtgatgacgg gaccgaaggc ggtgtgccga gccccttccg gcgtcgtgat cgacaggatg
     4501 ggcgtgccca cctcggcttg aatccggctg acgccctcct cgtgggaggc ccgcagttcg
     4561 gcgtcgtatc cggtggagaa accggactcc acaaggcttt ccgggaggtc gcagcggcga
     4621 agggcgtcgg cgatgtcgcc gaaccattcc cggtcggtcc cgtcgggatc ggtccacaac
     4681 gcggtgtgga actcgccgag cgcggcgtgg ccgtgcccgg tctgcacggc ggcggcgatg
     4741 cgggccggaa tccacaggaa cccgtcctcg tcgccctcgg ggtcgacgtc cttgccctcg
     4801 ttgagaatcg acaggctcat cacccgccag cgaatgtcga gcgggatctc gtccgcgacc
     4861 gtcacgagcc atcgtgccgt gacgcgggtg agcgggcagg ccgggtcgag ccagaagtcg
     4921 gcggcccacc ggggtgagtc tccggcggtg ctggtcgtca tggtttccat cctgccacca
     4981 cgagacagcg ccgggcctgg tcatccggcg gcggggtagg cgccgttctc gtcgtgtgac
     5041 tccgcgccga cgagtgccgg ggtgaacacg cagatgatgc gcaggtcggt gaccgctcgc
     5101 agcaggtgcc ggtcgtggcc gtcaaggacg tacatcgttc ccggtgagac atcgtgggac
     5161 cggccgctgc cgagatcgac caccacaccc tggccctcga cgcagaatac cgattcgctg
     5221 tgatgcgcgt actggatcag cagttcggta cccgcgtaga tggtcgagtc gtggaacgag
     5281 taaccgagcc ctgcccccgc gagcagcagc cggatgctgc gccagccggg cccgtgcacg
     5341 tcaccggccg ttccgatcgt gtccgcgaga ctcgtgacat acatgacgcg tcatcctttc
     5401 gttgctccgg ttaccgccag ggtcacgagg gcgcgagcga gggcaacagg cgcccgtgaa
     5461 gcgagcgcgc cagcgcgaca ccggccgcct cgtcgtcggc gaggtggacc tcgacccgct
     5521 cgccctcgat cccgacaacc ggtattcccg ccgggccgcc cagcgcgacc ccgcggcgct
     5581 cgcacacctc gccgagatcg acgtagccgc cgatcaccag cacgaccggt cctttcctgg
     5641 tatgtgcggc cagcgcgttg attccctccg ccgcgagttg cgccgcggtc tcctcggccg
     5701 aggagacgct cgggctggtg aaagcacgtg acagccggtt gagcacgacc gcgccgggct
     5761 cctcggcgga gtcgatgccc ccgatcaccg accctcgccg gtagggcggg gtgttggccg
     5821 cgcagacgat ccgaagacca tggcgcgcgg cgaggcgcac cgcctcgggg tgcagtacct
     5881 tcgcgccgta gcgggtgagc cgctgagcca gcgggtaggc gacctcgccg agcaaccggc
     5941 tttcctccac caccctgggg tcgcagctgt acacaccgtc gacgtcggag aagatctcgc
     6001 agtgggcggc gccagtcgcg acggcggcgg ccacggctga cagatccgag ctgttcttgc
     6061 ccagccacgt ggggcgtccg ctctcgtcgc cggcctgccc gccgggtagg acgacacagc
     6121 tgccgcgacg caacgcatcc cgcagtggtg ccgggtcgat gccgctgagc gttgcccaca
     6181 gcggggatcc tcttgtcgtg aatccggtcg cctgaccggt cagcacctcg gccacccgcc
     6241 cctcggcgtc gagcgcggcc gccagcagca gggcgctcat cgtgtcggcc atgggcaaca
     6301 ggctcaccag cgcgttttgc cctgggaccg cgcacgcggc gtgcacccgt tgcctgagcc
     6361 gctcggtgtc acccggcatc gcgctgacga cgacgacctg accttcgcct gattcggccg
     6421 ccctgcgccg gatcatcgcg gcgacctcgc ggtagcgcga cggcgtcgcg aacgtcgagc
     6481 cgccgaactt cagcacgctg accgcgcttc ctgggttcac agcttgcctt tctcgcggag
     6541 cagttcggcg atctggatgg cgttgagcgc ggcaccgatc cggaggttgt cggccacgag
     6601 ccacaaccag acgccgcatc cgtcctcgga aaccctgatc ctgccgacgt ggacgcggtc
     6661 cggatcgccg ttgtgtctcg gcgtcggcgt gctgaccggg tcggtcgatt cgtgcacgac
     6721 gacgtccggc tgggcggcca gttccgcgat caccgcctcg cgttcggcag gccgccgggt
     6781 gcggatccac accgcttcgc tgtgcccgtt gacgacggga acccggacac aggtggccgt
     6841 gacatcgagt tccggtgagg cgaggatctt cgccgattcc tgggccatct tgcgttcctc
     6901 cacggtgcct ccgtcgtcga ggaccgtgtc gatgaacggg atcacgttga aggccagccc
     6961 cggcgggaac tccttgaccg gtaccgaata cggcgggccc gcgagctgcg atcgggcgtc
     7021 ctcgcgcaac tccccgattc cggagtagcc accgcccgag gccgcctggt aggtgctaac
     7081 gacgacctgc tcggctcccc actcgcgtag taccggtcgc agcaccctga ccagcgggat
     7141 ggtggagcag ttcggattgg cgacgatccc gctggccggc cgccggtcca gtaccgaggc
     7201 gttcacctgt ggaacgacaa gcggtgtgtc agggtccatc cggaacgccg cggtgttgtc
     7261 gatcaccagc gcgccctcgg ccgaggcacg gggtgcccag atccggctca gtgccgtgcc
     7321 cgcggagaag aaagccacgt cgacggagcc aaaatcgacg tcgtcgatct cgcgcaccac
     7381 gagttcggcg ccgccgaact cgacggtcct gcctgccgag cgcggcgagg ccaccagttc
     7441 gaaggacgcg tagtcgaagc cgcgttcctc gacgagctgg agcagggaca taccaacggc
     7501 accggtcgcg ccgacgacgg cgacgcgggg cgcgtcgctc gcgtgctggg gacgatcact
     7561 ggccattcct cacacacctt cggcagtagc aggggcatcg gtgtcgaacc ttcgccgtac
     7621 atgggcgagc atccgcagca actcggctga ggtgacgcgc tgagcgcggg cgcgggggac
     7681 gaggtggctg atgggcatcg caccggggtc ggcccagatc agccgtcccc gctcgtcgat
     7741 gccggatctc gcgacgttcg cgttgtcagg gtgcaggcaa tagggcacgt cgagcacgcc
     7801 cccgcggaac gcgcggacca gcgcgccgcc gaccgtgtcg gcaagaccga gcacggtgtc
     7861 gatcagggcc cgcgcccttt cctcgatccc ggtgccagcg ggtgcggggg gcctgtcccg
     7921 ctgggcgaga gccgcgtcgt gcgcgtggcg cagcgcggcg acgttctcct cgatcgtcgg
     7981 aatgcgcgtc gcctcggcga ccgtcttgac gatcacccgc tcgcagcccg ccgcggcagc
     8041 cagccgcgcg ctttcggcga gcaaaccgag cgcgccgcgc ctgctcgcgg ggtacacgcc
     8101 catgtaggtg tagacgacga cgtgccagcc gacgtcggcg gcgaggtagc tcgacgcgag
     8161 ctcccgcaaa gccagcagcg cttcgaggtc ctgtccgaag tggacctgct gggcatagct
     8221 cagcgacacg tccctgatcc cattgtgctg aaagaacaag gcttcgagca cactgatcgc
     8281 gacgagcagg tcgggagggc agagctgccc gagcagacag ccaccgaacg tctcgacgtg
     8341 tccctggtac gcgccgagca gcgcgcacga gcgctcccac tcctcggtcg cgacgtccag
     8401 cggaaccctg ctgtagggca ggcagtagga caccgggcct ccctcggtga ccgtgattcc
     8461 cgcgtcgagc atcacctcga agatctcgaa gggccgcgcg gagccgtgcc gcatctggat
     8521 tccgaacccg ggaccggcga catcggcgag cagctcacgg gtccgtcgcg cgccgtgcgc
     8581 gacgatcggg tacccgttga gggtcctgcc ctctgccagc gccgaccgtg ccctcgcgtg
     8641 ctgattggtg cgggtgaagc tgtccagcgt gatcgtgccg accgtgggaa gtccgctgtc
     8701 gcgcaccgcg cgcagccccc gccgcatcag gtcgatgtcg gagaaaccca tcctgcattg
     8761 aacgacgagg ccgtcccttt ccaccggaac aggacttcgc tcgggcggcg aattcacctg
     8821 ataccgctcc cccgccgagt agtcggcaac ctttcagcaa cgccggaaaa ctgtttcagg
     8881 taatcgatga aatcaccgac tggttgctcg ggagaaaaca cggcgtctac accgctggac
     8941 agcatctctc gatttttccg gttttcgaga aatccttcag tggtgagctt tccgccgacg
     9001 acgatgcgca ttccggcaac ctctggccgc gccctgacgg cggccgcgca cgcggggatc
     9061 tcgatggcgc cgtgaccgtt gacgctgctc agcacgagca ggtcgggcgc cgtggccacg
     9121 cattcgccga cgacctcgcg gaacggaacg cacgggccga ggttgtgcac cacgaacccc
     9181 tgctcttcca acaacaactg gagatagacc aggttccagg tgtgcgcgtc ggacggtgtc
     9241 gtggccacga ccgccaccgg agcgggcacg atccgcttct ccggcacgcg gcgtagcgga
     9301 tctcgcacct gtatcgcact caacacgagt tcttcccatc attcacgatg aatgaagcgc
     9361 cttactccat tcattcttcc gataggcatc gacgagtcaa gtgaagcgtt gtcaagtttc
     9421 caacaaatct cggtcaacta tgtcaaattg tcgaatatct tcttgcgtct tccccgtcct
     9481 ggtccacacc atggtgacac cagcaacgga tcacggcgaa caggagtacc ggcagtgaat
     9541 cagcggcttt ccgagtcacc ggtcgcccgc ccctttcgca tccctggagc cgaccggccc
     9601 ggcgatctgc gtttcgccta tttcaacctc aaggggcacc ctcgtggcga attgatgctg
     9661 catcgcctga tcggagcggg gttcgtgccc agccttgtca tcgacgagga ctccccgctc
     9721 gcggcggggc tgctcgccgc tcagttccgc cagctcggcc aggtcgccgc tttcacgccg
     9781 ccgaccccgc tggagaccgt gtgcaagacg ctcgacatcc ggtacgagac ggtgggcaac
     9841 cacaacgacg ccgacaccgt ggccgtactg gcggaggccg cgccttccct cggagtactc
     9901 ggcgacacgc ggatcatgcg ggcgcgcgtc atcgacgcgc tgccgcacgg gatcgtcaat
     9961 gtgcaccccg gtctactgcc ctccgtccgc ggcaacaatc cttacctgtg gtcggtcgtg
    10021 cacggcctgc cccagggcgc gacggcgcac ctcatcgaca ccggtgtcga caggggcccg
    10081 atcatgttgg ccagagaact ggaactcacg gcgggcacaa cccttcccga gctgatccac
    10141 acgatcaaca cactgtgcgc cgatgtcgtc gtgaccgcgc tgagccagct gacctcgggt
    10201 acggcgacac tgaccgagca gcccgccgac gacgccgtca cgttcaggga ggcaaggccg
    10261 gagatctgga gcctcgtcga gacgatcctg cgcgaacgcg ccggcgcggc ggcgcggcgg
    10321 tgacggccgc catgaacgac acggacgtct tcgccatcga atccgaggtt cgcgcctaca
    10381 gccg
//
