LOCUS       NZ_CP016353            21490 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Prauserella marina strain DSM 45268 chromosome, complete genome.
ACCESSION   NZ_CP016353
VERSION     NZ_CP016353.1
KEYWORDS    RefSeq.
SOURCE      Prauserella marina
  ORGANISM  Prauserella marina
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Prauserella.
REFERENCE   1  (bases 1 to 6851918)
  AUTHORS   Ruckert,C., Albersmeier,A., Jiang,C.-L., Jiang,Y., Kalinowski,J.,
            Winkler,A. and Zotchev,S.B.
  TITLE     Complete genome sequence of Prauserella marina DSM 45268, isolated
            from ocean sediment of the South China Sea
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6851918)
  AUTHORS   Ruckert,C., Albersmeier,A., Jiang,C.-L., Jiang,Y., Kalinowski,J.,
            Winkler,A. and Zotchev,S.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2016) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Coverage                   :: 33.7x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002240355.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 02:59:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,595
            CDSs (total)                      :: 6,536
            Genes (coding)                    :: 6,497
            CDSs (with protein)               :: 6,497
            Genes (RNA)                       :: 59
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 14 of 39
            Pseudo Genes (incomplete)         :: 31 of 39
            Pseudo Genes (internal stop)      :: 1 of 39
            Pseudo Genes (multiple problems)  :: 6 of 39
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:50:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1308908
            Orig. end                         :: 1330398
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP016353.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..21490
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ranthipeptide"
                     /region_number="4"
                     /rules="(TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085
                     or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /tool="antismash"
     cand_cluster    1..21490
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /kind="single"
                     /product="ranthipeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21490
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11490](-)"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11490)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
     gene            1452..2309
                     /locus_tag="BAY61_RS06020"
                     /old_locus_tag="BAY61_05980"
     CDS             1452..2309
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985346.1"
                     /locus_tag="BAY61_RS06020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_05980"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_091799536.1"
                     /transl_table=11
                     /translation="MIEGRTVVDAHVHVPRLSTLKPSWLEWARRFSGPHPWREVYDGDG
                     DPVPGRLDALFEEEGVDRALLFCEYSPRATGIQPIEDNLPFVECNPERFRLVANVNPHL
                     HHPLASEVERQLDLGAVALKLHPVHGGFSPADKELYAAYHVCQERDVPVVLHSGTSSFP
                     GARTSFGNPELLSDVVEDFPGVRFVFAHGGRGWWYDVAAFLALAKDNVWLDLAGLPPKK
                     LPGYYARFDFARLATRFVFGTDWPGVPGTANNVRALASLGLPEDVLCDVLAGNAGKIYP
                     GLGG"
     gene            complement(2317..3117)
                     /locus_tag="BAY61_RS06025"
                     /old_locus_tag="BAY61_05985"
     CDS             complement(2317..3117)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334016.1"
                     /locus_tag="BAY61_RS06025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_05985"
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="WP_256327966.1"
                     /transl_table=11
                     /translation="MSARSLLMTLLGEFVLPRDRPVWTSVLVDALATFDVEEKSARQAL
                     ARTAKEGWLESERVGRRVRWSLTEPGRKLLTEGAHRIYDFGSSERTWDGRWLIVLVSVP
                     EAKRDLRHQLRTKLTWAGFGSPDPGVWITPHTSREKEALTILDALALGEAMSFVGSYGA
                     IGDESTVVTRAWDLRELVRRYDEFVAAFATLDPADEAAALDAQTRLVHEWRRFPFLDPQ
                     LPPGLLPGNWTGGRAAEVFHGKHAEWRPSAQRHWDRLVAVEGRE"
     gene            complement(3221..3898)
                     /gene="mftE"
                     /locus_tag="BAY61_RS06030"
                     /old_locus_tag="BAY61_05990"
     CDS             complement(3221..3898)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152862.1"
                     /locus_tag="BAY61_RS06030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_05990"
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="WP_091799542.1"
                     /transl_table=11
                     /translation="MTRLGGLRWPEVGKPVLAVPLGATEQHGPHLPLHTDTTIATELCD
                     RLAASIGDTVDIVVAPALPYGSSGEHAGFPGTLSIGRTALELLLVELVRSADDFAGVVI
                     VNGHGGNAAPVRAAVRLLRDEGRRVLAWSPDGPPGDSHAGRTETSVLLRLRPQDVATDK
                     AEAGNTAPLPELFDRLVAGGVAAVSVNGVLGDPAGADAAEGEALLTRWAEALATAVRDW
                     LGR"
     gene            complement(3895..5226)
                     /gene="mftF"
                     /locus_tag="BAY61_RS06035"
                     /old_locus_tag="BAY61_05995"
     CDS             complement(3895..5226)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152863.1"
                     /locus_tag="BAY61_RS06035"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_05995"
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="WP_091799545.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.7e-14,
                     bitscore: 52.6, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTGTLLTMDDSVRRRGPLLIGGSPLRLVRLGVGGAKVLDRWLGG
                     EPLGPAATEHRLARRLVDAGLLHPVPPPELTAAEVTLVVPVKDNPDGVARLVAATPELA
                     CHVVVDDGSNRPLPSAAIRHDTPRGPAAARNAGWRTANTRVVAFLDSDTLPEPGWLEPL
                     LRHFSDPAVVAVAPRIRGSQGHSALAAYEADRSSLDLGALPAPVRPMSRVSYVPSAALL
                     VRRDALAAVSGFDEALRFGEDVDLVWKLLELGAVRYEPTAVVGHLPRPSALPWLRQRFE
                     YGTSAAPLALRHPGLLSPARLSAWSAASWALLAAGAPRAALTLAAGSSALLPRKLRSRG
                     IPVRESLRLAALGHLGAGRLLAEAARRAWWPLLLPTARGRRLLLAALLPCVLEWLRERH
                     GRHPSWLALRVADDLAYGAGVWAGAVRERTAAPLLPRFTEGSLR"
     gene            complement(5223..6059)
                     /locus_tag="BAY61_RS06040"
                     /old_locus_tag="BAY61_06000"
     CDS             complement(5223..6059)
                     /NRPS_PKS="Domain: PKS_KR (21-195). E-value: 8.5e-11.
                     Score: 34.0. Matches aSDomain:
                     nrpspksdomains_BAY61_RS06040_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947180.1"
                     /locus_tag="BAY61_RS06040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_06000"
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="WP_245865850.1"
                     /transl_table=11
                     /translation="MSAPFPRDGAGRNGETMSQPRTALVTGAARGIGAAAVRRLADTGW
                     NVVALDRAEDDPSVPYPLGTRKQLAELAAPYQGAVHDVVADVRDLARLTEAVALAEERF
                     GGLDAAVAAAALMAGGAPLWESDDTEWDALFSVGVQGVANLARAAIPALLRRPEPRHGR
                     FVALASAAAHNGLWHLAGYNAAKHAVVGLVKGLATDLRGTGICATAVSPGSTRTDMLTA
                     TAALYDLTDVEEFAKHQLVGRLLEPDEIAATIAFLCSPDSSAITGSVIHADGGFTT"
     aSDomain        complement(5475..5996)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BAY61_RS06040_PKS_KR.1"
                     /evalue="8.50E-11"
                     /label="BAY61_RS06040_PKS_KR.1"
                     /locus_tag="BAY61_RS06040"
                     /protein_end="195"
                     /protein_start="21"
                     /score="34.0"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="TALVTGAARGIGAAAVRRLADTGWNVVALDRAEDDPSVPYPLGTR
                     KQLAELAAPYQGAVHDVVADVRDLARLTEAVALAEERFGGLDAAVAAAALMAGGAPLWE
                     SDDTEWDALFSVGVQGVANLARAAIPALLRRPEPRHGRFVALASAAAHNGLWHLAGYNA
                     AKHAVVGLVKG"
     CDS_motif       complement(5928..5990)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BAY61_RS06040_0001"
                     /evalue="6.50E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="BAY61_RS06040"
                     /protein_end="44"
                     /protein_start="23"
                     /score="12.4"
                     /tool="antismash"
                     /translation="LVTGAARGIGAAAVRRLADTG"
     gene            complement(6056..8023)
                     /locus_tag="BAY61_RS06045"
                     /old_locus_tag="BAY61_06005"
     CDS             complement(6056..8023)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152869.1"
                     /locus_tag="BAY61_RS06045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_06005"
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 2"
                     /protein_id="WP_091799549.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.7e-10, bitscore:
                     39.1, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKWYPHLFSPLRIGPVTLANRIVFSAHLTNYAGGGAGGLPTARH
                     AAYYAARAAGGAGLIITEEHSTHPADRPYEKLIRGHLPEVIEGYRRITDAVHAHGTPIF
                     AQLNHNGGQSSGLYSRLPLVAPSPVPDPVFREVPREATAADIDDIVAGYATVAAHCRRG
                     GFDGVEIQCSQSSIVRGFLAPATNIRTDEYGGSLWNRARLLLRLVAAVREAIGADMALG
                     IRLCGDELVEGGITLPEAIDVAGWAEATGGVDYVNTSIGVATSTLYLIEASMGIPRGYA
                     TYISSAIRDTVELPVVGVGRFKDPEQAEKALAEGKADLIGVVRGQIADADFAAKARSGH
                     SGAIRTCLSCNQECVGRVGLNRDLGCVENPRTGKESVPLPPPRRRGRRVVVVGGGPAGL
                     RTAATAASRGHQVTLLERLAATGGQVAIAARAPSRAEFLDLVSTSLTECLREGVDVRTG
                     FTATAESIAALAPEAVVLATGARPRRPHWAGELGRIVDVRDVLEGRAAPEGRVLVVDDL
                     GFHQATSVAELLADRGCAVRMSTPGLVVGQDLALTLDMETFSVRAEAKGIVQDTEEVIL
                     SATRDTGGVRLRVLRHTTGATTDACFDWVVCATHQEPEDTLWHELSGSAKFEVVRAGDC
                     VTPRRADAAIAEGHRAAVAL"
     gene            complement(8020..9957)
                     /locus_tag="BAY61_RS06050"
                     /old_locus_tag="BAY61_06010"
     CDS             complement(8020..9957)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152870.1"
                     /locus_tag="BAY61_RS06050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_06010"
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 1"
                     /protein_id="WP_091799552.1"
                     /transl_table=11
                     /translation="MTHSTQDTGLRPLTLPLRLAGRRAASRTLFGPHVTNLGTGRAIGE
                     AHVAYYERRAEGGCGVIVTETASVHESDWPYERAPLAAECGPGWTAITRACRPHGALVV
                     ASIGHTGSQGTSAYSQQALWAPSRVPDVASRELPMEMEAAHIRALIDGFDEAARIAVES
                     DVDGVEVDAGQYALLRQFLSGLTNQRNDAYGTDRTALLREVLSSVRKRIGEGRVLGLRL
                     SCDELAPWAGITPEQAPGIAARLVGDSGNIDYLTVVRGSALATARPDSHTEPGFNIGLC
                     RDVREAVSGAAAVVLQGSVVDPEQAASAIADGVADLVELTRAHIADADFVAKLGSPERI
                     RPCTLTNQKCGVRDVRNPVVSCIGDPRSGHETGDPPSEGTDPPRRALVVGGGPAGLEAA
                     RVLALRGHDVRLVEREAELGGMLNVAARIPGRARLGALRDWLAGEVIRLGVEVSTSTEV
                     DLGDKTGVIVATGSVAGPRNYLVHDGAVVLDVVGLLRDPRGLGPGPVVVHDPVGDAVGV
                     GVAELVAARGHAATIISQDLVIGTRLAPTGDLADATARLRRAGVTLAKRSVLREVFAGH
                     VVVTDAYTGTATDIPCSTLIHCGHRLPRPGAFPSSALLAGDRVAPRTVHEAILEGRRAA
                     LELGARTVTA"
     gene            complement(10001..11209)
                     /gene="mftC"
                     /locus_tag="BAY61_RS06055"
                     /old_locus_tag="BAY61_06015"
     CDS             complement(10001..11209)
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: SPASM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152871.1"
                     /locus_tag="BAY61_RS06055"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="BAY61_06015"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_091799555.1"
                     /sec_met_domain="PTHR43273 (E-value: 9.7e-30, bitscore:
                     102.3, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 3.2e-25, bitscore: 88.0,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 3.3e-08, bitscore: 31.8,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 6.5e-14, bitscore:
                     50.6, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 3.6e-05, bitscore: 22.7,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLVEHFQHGLDAPICLTWELTYACNLSCVHCLSSSGRRDPRELS
                     TEECKAVIDELQRLQVFYVNIGGGEPTVRADFWELVEYAVDHQVGVKFSTNGVRITPER
                     ARWLAATDYVDVQLSLDGATAEVNDHVRGKGSFDTAVKAMENLRDAGFTNFKISVVMTR
                     HNVGQLDDFKRLADEYKAQLRITRLRPSGRGADTWDELHPSADQQLTLYEWLVGNGDNV
                     LTGDSFFHLNALGSTPLPGLNLCGAGRVVCLIDPIGDVYACPFAIHDEFLAGNVRDEGG
                     FTSVWRTSELFDRLRAPQTGGACTSCSAYDSCQGGCMAAKFFTGIPLDGPDPECVKGNG
                     ERALALVGGGTDLPKPDKDHSRRGKKAARAKQVPVAIGWGPPEPDLLPDRACDTSPLAG
                     FRP"
     gene            complement(11206..11490)
                     /gene="mftB"
                     /locus_tag="BAY61_RS06060"
                     /old_locus_tag="BAY61_06020"
     CDS             complement(11206..11490)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806422.1"
                     /locus_tag="BAY61_RS06060"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; MftB, a small protein, is a peptide
                     chaperone that assists the radical SAM enzyme MftC in
                     performing two modifications to the C-terminal Val-Tyr
                     dipeptide of the mycofactocin precursor peptide, MftA.
                     MftB's role is analogous to the role of PqqD in the
                     biosynthesis of PQQ, a cofactor that derives entirely from
                     a Tyr and a Glu in the precursor PqqA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="BAY61_06020"
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="WP_091799558.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 8.5e-28,
                     bitscore: 95.0, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTEPFEAGRPYRLSSGVALRPEPFGALVYDFTTRKLSFLKTQLL
                     VDVVRDLENQPDALTTLGAAGVPDAQHPRYLDALAGLLSAGTIESRGDS"
     aSDomain        complement(11227..11463)
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_BAY61_RS06060_Mycofactocin_RRE.1"
                     /evalue="3.70E-30"
                     /identifier="RREFam016.1"
                     /label="BAY61_RS06060"
                     /locus_tag="BAY61_RS06060"
                     /protein_end="88"
                     /protein_start="9"
                     /score="95.0"
                     /tool="antismash"
                     /translation="RPYRLSSGVALRPEPFGALVYDFTTRKLSFLKTQLLVDVVRDLEN
                     QPDALTTLGAAGVPDAQHPRYLDALAGLLSAGTI"
     misc_feature    complement(11359..11361)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(11480..11569)
                     /gene="mftA"
                     /locus_tag="BAY61_RS06065"
                     /old_locus_tag="BAY61_06025"
     CDS             complement(11480..11569)
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983865.1"
                     /locus_tag="BAY61_RS06065"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="BAY61_06025"
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_091799561.1"
                     /transl_table=11
                     /translation="MNEAETTEVTVEEDLLVEEVSIDGMCGVY"
     gene            11645..12283
                     /gene="mftR"
                     /locus_tag="BAY61_RS06070"
                     /old_locus_tag="BAY61_06030"
     CDS             11645..12283
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0036042 - long-chain fatty acyl-CoA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="mftR"
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229847.1"
                     /locus_tag="BAY61_RS06070"
                     /note="MftR, the mycofactocin system transcriptional
                     regulator, is an uncharacterized TetR family DNA-binding
                     transcription factor. Its role is inferred by context. It
                     occurs as part of the biosynthesis locus for mycofactocin,
                     a partially characterized electron carrier derived from the
                     terminal Val-Tyr dipeptide of the precursor peptide MftA,
                     through a radical SAM enzyme-mediated process.; MftR, the
                     mycofactocin system transcriptional regulator, is an
                     uncharacterized TetR family DNA-binding transcription
                     factor. Its role is inferred by context. It occurs as part
                     of the biosynthesis locus for mycofactocin, a partially
                     characterized electron carrier derived from the terminal
                     Val-Tyr dipeptide of the precursor peptide MftA, through a
                     radical SAM enzyme-mediated process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="BAY61_06030"
                     /product="mycofactocin system transcriptional regulator"
                     /protein_id="WP_245865852.1"
                     /transl_table=11
                     /translation="MVTSPPELPVRPGRRRSTSRQQLESVAFELFELHGFDATTVDDIA
                     KGAGIGRRTFFRYFESKNDVVWGAFSEQLDRMRLQFDSRPADEPLMASLRAVVVEFNRV
                     EPEEEPRHRRRMELILRVPALLAHSTLRYGEWRDVVAEFAAGRLGERPESLRPQAIAHA
                     ALGVALAGYQHWLGEGGVERVALCDVLDAAFADLAEGFAPPPGEPTREA"
     gene            complement(12267..12728)
                     /locus_tag="BAY61_RS06075"
                     /old_locus_tag="BAY61_06035"
     CDS             complement(12267..12728)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160157.1"
                     /locus_tag="BAY61_RS06075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_06035"
                     /product="GreA/GreB family elongation factor"
                     /protein_id="WP_091799567.1"
                     /transl_table=11
                     /translation="MTSTGGLSPATRQRLENELEQIREQRSALAARLGDDPLGDSADQA
                     ELLGRAEHASRLDQRIAEITDLLHGGPIPDSDSALPSGTQVTLKFADGSVEEMVVVGIA
                     EEAGDDLSALTADSPLGKAIAGHKKGDKFTYRTPAGEASAEIVELKPPA"
     gene            complement(12783..15149)
                     /locus_tag="BAY61_RS06080"
                     /old_locus_tag="BAY61_06040"
     CDS             complement(12783..15149)
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661992.1"
                     /locus_tag="BAY61_RS06080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_06040"
                     /product="Tex family protein"
                     /protein_id="WP_176879620.1"
                     /transl_table=11
                     /translation="MVSIDQKIAEELGVGAGQVQAAVGLLDGGSTVPFIARYRKEVTGA
                     LDDTQLRTLEERLRYLRELEERRTTILDSIREQGKLDEALEARIMAADSKARLEDIYLP
                     YKPKRRTKAQIAREAGLEPLADGLLSDPSTEPQAAAAVFVDAEKGVADTAAALDGARAI
                     LVERFAEDADLIGELRERMWTRGWVTSKVRQGKETEGAKFADYFEFAEPFTKLPSHRIL
                     ALFRGEKEEVLDLTTDPGEPGSDNPGADYETRIAARFGVSDEGRPADKWLSDTVRWAWR
                     TKILLHLGIDLRMRLRQAAEDEAVRVFAANLRDLLLAAPAGTRATMGLDPGYRTGVKVA
                     IVDGTGKVVTTDTIFPHKPQSKWDESLVKLEKLAREHNVQLIAIGNGTASRETDKLAGE
                     LVKLRPELKLTKIVVSEAGASVYSASAYASQELPGMDVSLRGAVSIARRLQDPLAELVK
                     IDPKSIGVGQYQHDLAESKLSRSLDAVVEDCVNAVGVDLNTASVPLLSRVSGIGTGLAE
                     NIVAHRDGNGPFRSRTALKEVARLGPKAFEQCAGFLRIPDGDDPLDASSVHPESYPVVH
                     RILDETKVGIAELIGNPRALRGLDPKTFADDTFGLPTVTDIFAELEKPGRDPRPEFTTA
                     TFAEGVETMGDLKQGMTLEGVVTNVAAFGAFVDVGVHQDGLVHVSAMSRTFVKDPREVV
                     KPGDVVKVKVLDVDVPRKRISLTLRLDDDTSPGQGQRGGGPRGQSGQQGQRGSGNQGQG
                     GQRKGKQQGGKQRDDRKAPAGGAMADALRKAGYGK"
     gene            15221..16894
                     /gene="ilvD"
                     /locus_tag="BAY61_RS06085"
                     /old_locus_tag="BAY61_06045"
     CDS             15221..16894
                     /EC_number="4.2.1.9"
                     /GO_function="GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ilvD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026113.1"
                     /locus_tag="BAY61_RS06085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_06045"
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="WP_091799573.1"
                     /transl_table=11
                     /translation="MADRDRKPRSTEVTDGIERAAARGMLRALGMGDDDFAKPQIGIAS
                     SWNEITPCNLSLRRLGDAAKLGVSGAAGFPMEFGTISVSDGISMGHEGMHFSLVSREVI
                     ADSVETVMEAERLDGAVLLAGCDKSLPGMLMAAARLDVAAVFLYAGSTLPGTVDGREVT
                     IIDAFEAVGACARGLIGRDEVERIEKAICPGEGACGGMYTANTMACAAEALGMSLPGSA
                     SPPSVDRRRDRFARASGEAVVGLIDAGVTARDILTREAFENAIAVVMALGGSTNAVLHL
                     LAIAHEADVELTLGDFARIGDRVPHLADVKPFGRHVMTAVDRVGGVPVVMKALLDAGLL
                     HGDCRTVTGKTVAENLAELAPPGLDGEVIRKLADPIHPTGGLTILHGSLAPEGAVVKSA
                     GFDSARFEGTARVFDGEQAAMNALGELKAGDVVVIRNEGPRGGPGMREMLAVTGAIKGA
                     GLGKDVLLLTDGRFSGGTTGLCIGHVAPEAAHGGPIAFVRDGDPIVLDMTARTLDVKVG
                     EQEMARRAEGWRMPEVPRRTGVLAKYAKLVGSAANGAVCT"
     gene            complement(17201..17764)
                     /locus_tag="BAY61_RS06090"
                     /old_locus_tag="BAY61_06050"
     CDS             complement(17201..17764)
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /GO_function="GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_process="GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444018.1"
                     /locus_tag="BAY61_RS06090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_06050"
                     /product="pyridoxamine 5'-phosphate oxidase family protein"
                     /protein_id="WP_091799576.1"
                     /transl_table=11
                     /translation="MKVHEGIDARLSRFLLDQPVFFVGTAPTSDEGHINVSPKGMRGTF
                     AVFGEHEVGYLDYTGSGAETLAHLRENGRIVLMFCAFTGPPNIVRLHGRGRAVLPGSPG
                     FGELLARFDTGEHQGLRAIIDVEVTRVSDSCGFSVPLLGYTEDRDLLLRRHSRRSDEDL
                     AEYWATRNAHSIDGLPALDPPGPP"
     gene            complement(17767..21345)
                     /gene="cobN"
                     /locus_tag="BAY61_RS06095"
                     /old_locus_tag="BAY61_06055"
     CDS             complement(17767..21345)
                     /EC_number="6.6.1.2"
                     /GO_function="GO:0051116 - cobaltochelatase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="cobN"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1263: cobaltochelatase subunit CobN"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640444.1"
                     /locus_tag="BAY61_RS06095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BAY61_06055"
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="WP_091799579.1"
                     /transl_table=11
                     /translation="MILLLSTSDTDLLSAKASAAGYRLANPSRLDLAELPDLLTGATVV
                     VVRILGTERTWADGLRLVRDSGAHVIVLGGEQTPDAELMALSTAPAGIAAEAHAYLAHG
                     GPGNLAQLHRFLSDTLLLTGEGFEQPVEQPSWGVLERPDSGGPTIAILYYRAHHLSGNT
                     AFVETLAEQIEAAGGRALPVYCASLRSREPAMLAELRKADALLVTVLAAGGTRPSEAGA
                     GGDDESWDVAELAALDVPTLQALCLTSDRETWLGNDEGLSPMDAGNQMAVPEFDGRLIT
                     VPFSFKEFDADGLPRYVPDAERASRVAGIALAHARLRHTPARDRKIVLMLSAYPTKHSR
                     VGNAVGLDTPASAVKLLRAMRELGYDLGEDAFPGVVPTGTDQPDGDALIHALIEAGGQD
                     PEWLTEEHLSGNPIRVPASRYRQWFAALPDDLREDMVEHWGPPPGDLYVDRSGDGDGDI
                     VLAALRAGNVVLMIQPPRGFGENPVAIYHNPDLPPSHHYLAAYRWLEEEFGAHAVVHLG
                     KHGSLEWLPGKTAGLSASCAPDAVLGNLPLIYPFLVNDPGEGAQAKRRAHATIVDHLIP
                     PMARAESYGDLARLEQLLDEHANIAAMDPAKLPAIRQQIWTLVQAAKLDHDLGVEQRPH
                     DAEFDDFLLHIDGWLCEVKDAQIRDGLHILGEAPSGEARVNLVLAMLRAQQIWGGKQGA
                     VAGLRTALGLTENAALSEVDAIEQRARDLVVAMEDNGWNAAAVETVCADAPAAVPVLRF
                     AAEEIVPRLAGTSGELTSVLHALDGGYVPAGPSGSPLRGLVNVLPTGRNFYTVDPKAIP
                     SRLAWETGQALADSLLTRYREDTGDWPRSVGLSVWGTSAMRTSGDDAAEVLALLGVRPV
                     WDEASRRVTGVEAIPLAELGRPRIDVTVRISGFFRDAFGHVIDLLDDAVRLVAGLDEPE
                     ESNFVRAHVRADLDSHGDERRATTRIFGSKPGSYGAGLLPLMDSGNWRDDSDLAEVYAV
                     WGGYAYGRDLDGAPARADMESSYRRIVVAAKNTDTREHDIADSDDYFQYHGGMIATVRA
                     LTGTAPTSYVGDSTSPDAVRTRTLGEETTRVFRARVVNPRWLAAMRKHGYKGAFELAAT
                     VDYLFGFDATAGVVQDWMYEQLTESYVLDKENQEFLAQANPWALRGIIERLDEAVDRGL
                     WAEPDAELLDKMRAVYLRIEGDLEGE"
     misc_feature    21363..21376
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.96"
                     /tool="antismash"
ORIGIN
        1 acctattccg aactcgatca gcaggtccgc acggtggcag cgggcttgcg cgcgctcggt
       61 gtccgcccgg aagagcgggt gctgatgtgc atgaccgacg agatcgagtt gttcaccgcg
      121 attctggccg ggatgtatct cggcgcggtc gcggtgccgt gctcgacgat gctgaccggc
      181 ggcgaactcg gccggttgat cgccgattcc agaaccagga tcgtgctggg gtcgacggag
      241 ttcgccgagc aggtgcgcgc gggcgtcgcg gtcgcgccgg acgtggaaac ggtggtgctg
      301 accgatgagt ggcaccggct caccggccag ggcgcgctct ccacgcccta cgacacctgg
      361 gccgactcac ccgcgctgtg gctctacacc tcgggcacga ccggggcgcc caaggccgcg
      421 atgcaccggc acaccgacat caggttcgtc gccgagaact acgcgcagcg cgtgctcggc
      481 atccggccgg acgacaggtg tctttcggtg gcgaagctct tcttcgccta cggcatcgga
      541 aattccatgt tcttcccgct ttccgtcggt gccaccgcgt tgctggaatc ggcgaggccg
      601 actccggcgc tgatggcgga gcgggccagt acccagaacg cgactctgct gttcggtacg
      661 cccagcttct gggggccgct gctggccagc gacgttcccg gcgacgcgtt ccggaccgtg
      721 cgtggcggcg cgtcggccgg ggaggcgctg cccccgcgca tgctgcacgg aatgcgggaa
      781 cggttcgggg tcgaggtact cgacggcatc ggctcgaccg agatgctgca catcttcatc
      841 tccaaccggc ctggcaaggc gcggccgtcg tcgtcgggga cgcccgttcc cggctacgag
      901 gtggagatcc gcgacgagag cggcgcgctg atcgagaccg atgggcagcc gggggagctg
      961 tacgtgcgcg gggacagcgc ggcgacgggc tactggtgca gggcggcgac ctcgcggctg
     1021 gtgttcctcg gtgagtggat gcgtacgggc gacacctacg tgcgcaacgc cgatggcacc
     1081 tatacgtgtc tcggcaggtt caacgacatg atcaaggcgg gcggtatctg ggtcacgccg
     1141 agcgaggtcg aggaccggtt gctcgaacat ccggacgtcg cggaggcggt cgtggtgggt
     1201 gttcccgatt ccgacgagct cgacaagccg gtggcgtgcg tggtgccgat gccgggaaag
     1261 catgtcgacg aagcggacct gatccggtgg tgcagggagg aattggcttc cttcaagcgg
     1321 ccgagggtgg tggtcgagct ggccgagttg ccgaagaccg cgaccggcaa gatccgcagg
     1381 aacgtgttgc gggacatggt gaaagccact tcggagccgg acccggacac acagcccgcc
     1441 gaggcggccc cgtgatcgaa ggcaggaccg tcgtcgacgc gcacgtccat gtgccgaggc
     1501 tgtcgacgct gaagccgtcg tggctggagt gggcaaggcg gttctcgggc ccgcacccgt
     1561 ggcgcgaggt ctacgacggc gacggtgatc cggttcccgg acggctcgac gccctcttcg
     1621 aggaagaagg cgtcgaccgt gccctgctgt tctgcgagta cagcccgagg gcgaccggca
     1681 ttcagcccat cgaagacaat ctgccgttcg tcgagtgcaa tccggaacgg ttccggctcg
     1741 tcgcgaacgt gaacccgcat ctgcatcatc cgctggcgtc cgaagtggaa cgacagctcg
     1801 atctcggtgc cgtcgccctc aaactgcatc ccgtgcacgg tggtttctct cccgccgaca
     1861 aggagttgta cgcggcgtac cacgtgtgcc aggagcggga cgttcccgtt gtcctgcatt
     1921 cggggacgag cagtttcccc ggcgcgcgca cgagtttcgg caatcccgag ctgttgtcgg
     1981 atgtcgtcga ggactttccg ggagtgcggt tcgtgttcgc gcacggcggc cgtggctggt
     2041 ggtacgacgt cgcagcgttt ctcgcgctcg cgaaggacaa cgtatggctc gatctcgcgg
     2101 gactaccgcc gaagaagctg ccgggctact acgcgcggtt cgacttcgcg aggctggcga
     2161 cccggttcgt gttcggcacc gactggccgg gtgttccagg gacggcgaac aacgtcaggg
     2221 ctctcgcctc gctcggcctt cccgaggatg tgctgtgcga cgtcctcgcg ggcaacgcgg
     2281 gcaagatcta ccccggtctc ggcggatagc gcgacgtcat tcgcgacctt cgacggcgac
     2341 gagccgatcc caatgccgct gcgcggacgg ccgccattcg gcgtgcttac cgtggaacac
     2401 ctcggccgca cggccacccg tccagttgcc gggcagcagg ccgggcggca attgcgggtc
     2461 gaggaaagga aaccggcgcc attcgtgcac gagccgggtc tgcgcgtcga gcgcggcggc
     2521 ctcgtcggcc ggatcgaggg tggcgaacgc cgcgacgaac tcgtcgtagc ggcgcaccag
     2581 ttcccgcagg tcccatgccc tggtgacaac ggtgctctcg tcgccgatcg ctccgtagga
     2641 gccgacgaac gacatcgcct cgccgagcgc gagggcgtcg agaatggtca gcgcttcctt
     2701 ttccctcgac gtgtgcgggg tgatccacac gccggggtcg ggcgaaccga aaccggccca
     2761 ggtgagtttg gtccgcagtt ggtgccgcag gtcgcgtttg gcctctggca cggaaacgag
     2821 cacgatcagc cagcgtccgt cccaggtgcg ctcgctgctt ccgaagtcgt agatgcggtg
     2881 ggcgccctcg gtgagcagct tccggcccgg ctcggtcagc gaccagcgca ccctgcgccc
     2941 gacgcgctcg gattccagcc agccttcctt cgccgtcctc gccagcgcct ggcgcgcgga
     3001 cttctcctcg acgtcgaacg tcgcgagcgc gtcgacgagc acggatgtcc acaccggacg
     3061 gtcgcgggga agcacgaact cgccgagcag tgtcatcagc aatgagcgcg cgctcatgtg
     3121 actgacttcc ctgcggcggc tgacgacagg gcctccttgc cgcccctctg ctggcatggt
     3181 gacaccactt cctcgctgat cgggtgcgtc gagggtagcg tcagcgcccc agccagtcgc
     3241 gcacggcggt ggccagcgcc tcggcccacc gcgtgagcag tgcctcgccc tcggcggcgt
     3301 ccgcgccggc gggatcgccg aggacgccgt tgacactgac ggcggcgact cctcccgcga
     3361 cgagccggtc gaacagttcc ggcagtggag cggtgttacc cgcctccgcc ttgtcggtgg
     3421 cgacgtcctg agggcgaagc cgcagcagca ccgacgtctc ggtgcggccc gcgtggctgt
     3481 cgccaggtgg cccgtcaggc gaccaggcga gcacgcggcg cccctcgtcg cgcaaaagcc
     3541 ggaccgccgc gcgcaccggg gcggcgttgc ccccgtgccc gttgacgatc acgacaccgg
     3601 cgaagtcgtc ggcggagcgg acgagttcga cgagcaggag ttccagcgcc gttcttccga
     3661 tggagagggt gccgggaaat ccggcgtgct caccgctcga cccgtacggc agcgcgggcg
     3721 ccacgacgat gtcgacggtg tcgccgatcg aggcggcgag ccggtcgcac agttcggtcg
     3781 cgatggtggt gtcggtgtgc agtggcagat gcgggccgtg ttgctcggtg gcgccgagtg
     3841 gaacggcgag cacgggcttg ccgacctccg gccagcgcag cccgccgagg cgcgtcaccg
     3901 gagcgagcct tcggtgaagc ggggcagcag tggcgccgcg gtgcgttccc gcaccgcgcc
     3961 tgcccacacc cctgccccgt aggcgagatc gtcggcgacg cgaagggcga gccacgacgg
     4021 gtgcctgccg tgccgttcgc gcagccattc gagcacgcat ggcagcagcg cggcgagcag
     4081 gaggcgcctg cctcgggcgg tgggcagcaa cagcggccac cacgctctcc gcgcggcttc
     4141 ggcgagcagc cttcccgcgc cgaggtgacc gagcgcggcc agccggagcg attcgcggac
     4201 cgggatgcct cgggagcgga gtttccgggg cagcagcgcg gaactccctg cggcgagcgt
     4261 caacgcggcg cgaggggcac cggcagcgag cagcgcccac gacgccgcgc tccacgccga
     4321 gagccgggcg ggggagagca gcccgggatg gcgcagggcc agcggagcgg cggaggttcc
     4381 gtattcgaac cgctggcgca gccacggcag cgctgagggc cgtggcaggt gaccgacgac
     4441 ggcggtgggc tcgtagcgca ccgcgccgag ttcgagcagc ttccacacca ggtcgacgtc
     4501 ctcgccgaac cgcagcgcct cgtcgaagcc cgacacggcg gccagcgcgt cgcggcggac
     4561 gagcagcgcc gcgctgggca cgtagctcac cctgctcatg ggacggaccg gggcgggcag
     4621 ggcgccgagg tcgagactgg accggtcggc ttcgtaggcc gcgagcgcgg agtggccttg
     4681 gctgccgcgg atgcgcggtg ccacggcgac gacggcaggg tcggagaagt gccgcagcag
     4741 tggttcgagc cagcctggtt ccggcagggt gtcggagtcg aggaaggcca ccacgcgggt
     4801 gttcgccgtg cgccagcccg cgttgcgggc ggcggcggga cctcgcggtg tgtcgtgccg
     4861 gatggccgcg gacggcaacg gtcggttgga gccgtcgtcg acgacgacgt ggcaggcgag
     4921 ttccggggtc gccgcgacga gccgcgccac gccgtccggg ttgtccttca cggggacgac
     4981 gagggtgacc tcggccgcgg tcagttccgg cggagggacg ggatgcagca ggcccgcgtc
     5041 gacaagtcgc cgcgccagcc ggtgttcggt cgccgcgggg ccgagcggtt cccctccaag
     5101 ccaccggtcg agtaccttcg cgccgcccac gccgagccgc acgagccgca gtggggaacc
     5161 gccgatcagc agcgggcctc tcctgcggac cgagtcgtcc attgtcagca gcgttccggt
     5221 cttcatgtcg tgaacccacc gtcggcgtgg atgacggacc cggtgatcgc ggaggaatcg
     5281 ggtgagcaca ggaacgcgat cgtcgcggcg atctcgtccg gttcgagcag cctgccgacg
     5341 agctggtgct tcgcgaactc ctcgacgtcg gtgaggtcgt agagcgcggc cgtcgcggtg
     5401 agcatgtcgg tgcgggtcga cccaggggat accgccgtgg cgcagatacc ggtgccgcgc
     5461 aggtcggtgg cgagtccttt gacgagcccg acgacggcgt gcttggccgc gttgtaaccg
     5521 gcgaggtgcc acaacccgtt gtgcgcggcg gccgaggcga gcgcgacgaa cctgccgtgc
     5581 cggggctcgg ggcggcgcag cagcgcggga atcgccgctc tggccaggtt cgcgacccct
     5641 tggacgccga cggagaacag cgcgtcccat tcggtgtcgt cgctttccca cagtggggcg
     5701 ccgcctgcca tgagcgccgc ggcggcgacg gcggcgtcga gtccgccgaa ccgttcctcc
     5761 gcgagcgcga cggcctcggt gagccgggcg agatcccgca cgtcggcgac gacgtcgtgc
     5821 accgcgccct gatagggcgc ggcgagttcg gcgagctgct ttctggtgcc gagcgggtag
     5881 ggcacgctcg ggtcgtcctc ggctctgtcg agcgcgacga cgttccagcc ggtgtcggcg
     5941 agcctgcgga cggcggcggc gccgattccc ctcgccgcac cggtgacgag cgctgttctc
     6001 ggctggctca tggtttcccc gttccgtccg gcaccgtctc gcgggaatgg tgcgctcata
     6061 gtgccaccgc cgcccggtgc ccctcggcga tcgcggcgtc cgctctgcgc ggcgtgacac
     6121 agtcaccggc cctgacgacc tcgaacttcg ccgaaccgga cagttcgtgc cacagggtgt
     6181 cctccggctc ctgatgcgtc gcgcacacca cccagtcgaa gcacgcgtcg gtggtcgctc
     6241 cggtggtgtg cctcagcacg cgcagccgca ctccgccggt gtcgcgcgtc gcggacagga
     6301 tcacttcctc cgtgtcctgg acgatgccct tcgcctcggc gcgcactgag aaggtctcca
     6361 tgtcgagggt gagggcgagg tcctgcccga cgacaaggcc gggggtcgac atccgcaccg
     6421 cgcagccacg gtcggcgagg agttcggcca ccgatgtcgc ctggtggaaa ccgaggtcgt
     6481 cgacgacgag tacccggcct tccggggccg cccttccttc caggacgtcc cgcacgtcca
     6541 cgatcctgcc gagttcgcct gcccagtgcg gtcgccgtgg tctggcaccg gtggccagca
     6601 cgacggcttc cggcgcgagc gccgcgatgc tctcggccgt cgcggtgaag ccggttctga
     6661 cgtcgactcc ctcacgcagg cactcggtga gggaggtact gacgaggtcg aggaattcgg
     6721 ccctgctcgg cgcgcgggcc gcgatcgcga cctggccgcc ggtcgcggcc agccgttcga
     6781 gcagcgtcac ctggtggccc ctgctcgcgg cggtcgccgc ggtccgcaaa ccggcgggcc
     6841 cgccaccgac gacgaccacc ctgcggcccc tgcggcgcgg cggcggcagt ggcacgcttt
     6901 ccttgccggt gcgcgggttc tcgacacagc cgaggtcccg gttgagccct actctgccga
     6961 cgcattcctg gttgcacgaa aggcaggtcc ggatggcccc ggagtgtccc gagcgcgctt
     7021 tcgcggcgaa gtcggcgtcg gcgatctgtc ctcgaacgac accgatcagg tcggctttcc
     7081 cttcggcgag cgccttttcc gcctgctcgg ggtccttgaa gcggccgacg ccgacgacag
     7141 gcagctcgac ggtgtcgcgg atggcgctgg agatgtaggt cgcgtagccg cgagggatgc
     7201 ccatcgaggc ttcgatgagg tagagcgtcg aggtcgcgac accgatcgag gtgttgacgt
     7261 agtcgacgcc gccagtggcc tccgcccatc cggcgacgtc gatggcttcc gggagggtga
     7321 ttccgccttc gacgagttcg tcgccgcaca gccggatccc gagtgccatg tccgctccga
     7381 tggcttcccg cacggcggcg acgaggcgaa gcagcagccg ggccctgttc cacagcgagc
     7441 cgccgtactc gtcggtgcgg atgttcgtgg ccggggcgag aaatccccgc acgatcgagg
     7501 attgcgagca ttggatctcg actccgtcga agccgcctcg tcggcagtgc gccgcgaccg
     7561 tcgcgtaacc ggcgacgatg tcgtcgatgt cggcggcggt ggcctcgcgc ggaacctcgc
     7621 ggaacaccgg atcgggcacg ggggagggcg ccactagcgg tagccgcgag tacaggcccg
     7681 acgactggcc gccgttgtgg ttgagctgcg cgaagatcgg tgttccgtgt gcgtgcacgg
     7741 catcggtgat ccgccggtag ccctcgatca cctcgggcag gtgacctcgg atgagctttt
     7801 cgtagggccg gtcggcgggg tgggtcgaat gttcctcggt gatgatcagc ccggcgcccc
     7861 ctgccgcgcg cgccgcgtag taggcggcgt gccgcgcggt cggcagcccg cctgccccgc
     7921 cgcccgcgta gttcgtcagg tgtgccgaga agacgatccg gttggccagg gtcaccggcc
     7981 cgatccgcag cggggagaac aggtgcggat accacttgct catgccgtca ccgtcctcgc
     8041 gccgagttcc agcgccgcgc gccgcccttc gaggatcgcc tcgtgcacgg tgcggggcgc
     8101 gacccggtct cctgcgagta gcgcggagga agggaaagcg ccgggccggg gcagccggtg
     8161 tccacagtgg atcaacgtgg agcagggaat atcggtcgcc gtccccgtgt acgcgtcggt
     8221 cacgacgacg tgccccgcga acacttctct caggacgctg cgtttggcaa gcgtgacacc
     8281 ggctcgccgc aaccgggcgg tggcgtcggc gaggtcaccc gtcggcgcga gccgggtgcc
     8341 gatcaccagg tcctggctga tgatcgtggc cgcatgccct cgcgccgcga cgagttccgc
     8401 gacaccgact cccacggcgt caccgaccgg gtcgtgcacc acgaccggcc cggggccgag
     8461 accgcgcgga tcgcggagga gcccgacgac gtcgagcacg accgcgccat cgtgcacgag
     8521 gtagtttctc ggtcccgcta cggatcccgt cgcgacgatg acgccggtct tgtcgccgag
     8581 atccacctcg gtcgaggtgc tgacctcgac acccagccgg atcacctccc cggcgagcca
     8641 gtccctgagc gcgccgagcc gcgcgcgccc ggggatcctg gccgcgacgt tcagcatgcc
     8701 gcccagttcg gcttcccttt ccaccagccg gacgtcgtgt ccccgcaggg cgagcacccg
     8761 ggccgcttcc agcccggcgg gaccgccgcc gacgacgagc gcgcggcgtg gtggatcggt
     8821 tccctcggag ggagggtcgc cggtctcgtg cccgctgcgc gggtcgccga tacagctcac
     8881 cacgggattg cggacgtccc tgaccccgca tttctggttg gtcagcgtgc acggcctgat
     8941 ccgttcgggc gagccgagct tggcgacgaa gtcggcgtcg gcgatgtgcg ccctggtcag
     9001 ttcgaccaga tcggcgacgc cgtcggcgat cgccgaggca gcctgttccg gatcgacgac
     9061 cgagccctgc aacacgaccg ccgccgcgcc ggagaccgct tctctgacgt cccggcacag
     9121 cccgatgttg aagccgggtt cggtgtgcga atcgggtctc gccgtcgcca aggccgaacc
     9181 tcgtacgacc gtgaggtagt cgatgtttcc cgagtctcct acgaggcgag cggcgatgcc
     9241 gggcgcctgc tccggcgtga tccctgccca cggcgcgagt tcgtcgcacg agagccggag
     9301 gccgaggacc cggccctccc cgatgcgctt cctcaccgag gagagcacct cgcgaagcag
     9361 cgcggtccta tcggtcccgt aggcgtcgtt tcgctggttg gtgagtccgg aaaggaactg
     9421 gcgcaacagc gcgtactggc ccgcatcgac ctccacacca tccacatcgg actcgacggc
     9481 aatgcgtgcc gcttcgtcga agccgtcgat cagcgcgcgg atgtgcgcgg cctccatctc
     9541 catcggcagc tcgcggctgg cgacgtcggg gacccgcgac ggtgcccaca gtgcctgctg
     9601 ggagtaggcg gaggtgccct gcgagccggt gtgcccgatg ctcgcgacga cgagtgcccc
     9661 atgagggcgg caggcccgcg tgatcgccgt ccagcccggc ccgcactcgg cggcgagcgg
     9721 ggcacgttcg tagggccagt ccgattcgtg cacgctcgcg gtctcggtga cgatgacgcc
     9781 gcagccgccc tcggctctgc gctcgtagta ggcgacgtgt gcctcgccga tggctcttcc
     9841 ggtgccgagg ttcgtcacgt gcgggccgaa cagcgtccgc gaggcagccc ttcgccccgc
     9901 gagccgcagc ggcagggtca atggccggag gccggtgtcc tgagtggagt gggtcaccgg
     9961 ccgcgattcc cgcgagcagc cggtgacctc ccgcgaggaa tcaaggacgg aagcccgcga
    10021 gcgggctcgt gtcgcatgct cggtcgggaa gcaggtccgg ttcgggaggg ccccagccga
    10081 tcgcgaccgg aacctgcttc gcccgcgccg ctttctttcc ccgccgcgag tggtccttgt
    10141 ccggcttcgg taggtcggtt ccacctccga cgagcgccag cgccctctcc ccgtttccct
    10201 tgacacactc ggggtcgggg ccgtcgaggg ggattccggt gaagaacttc gcggccatgc
    10261 acccgccctg gcacgagtcg taggcggaac acgatgtgca cgcgccgccg gtctgcgggg
    10321 cgcgcagccg gtcgaacagt tccgacgtcc gccagacgga ggtgaagccg ccctcgtcgc
    10381 gcacgttgcc cgcgaggaat tcgtcgtgaa tggcgaacgg gcaggcgtag acgtcgccga
    10441 tcgggtcgat caggcagacg accctgccgg ctccgcacag gttcaggccg ggcaacgggg
    10501 tggagccgag cgcgttgagg tggaagaacg agtcgccggt gagcacgttg tcaccgttgc
    10561 cgaccagcca ctcgtacagc gtgagttgtt gatcggcgct cgggtgcagc tcgtcccagg
    10621 tgtcggcgcc cctgccggac gggcggagcc tcgtgatccg caactgtgcc ttgtattcgt
    10681 cggcgaggcg tttgaagtcg tcgagctggc ccacgttgtg ccgtgtcatc acgacactga
    10741 tcttgaagtt ggtgaagccc gcgtcgcgca ggttctccat ggccttgacg gcggtgtcga
    10801 aggagccctt accgcgtacg tggtcgttga cctcggccgt ggccccgtcg agcgaaagct
    10861 gcacgtcgac gtagtcggtc gcggcgagcc agcgcgctcg ctcgggcgtg atccgaacgc
    10921 cgttggtgga gaacttgact cccacctggt gatcgacggc gtattcgacg agctcccaga
    10981 agtccgcgcg gaccgtgggc tcgccgccgc cgatgttgac gtagaagacc tgcaaccgct
    11041 gtagctcgtc gatgacggcc ttgcattcct cagtggacag ttcacgggga tcgcggcggc
    11101 ccgacgagga aaggcagtga acacaggaaa ggttgcaggc gtaggtcaat tcccaggtca
    11161 aacagatggg cgcgtcgagg ccgtgctgga aatgctcgac caacttcatg agtcacctct
    11221 ggattcgatc gtgcccgccg acagcaatcc ggcgagcgcg tccagatagc gcggatgttg
    11281 cgcgtcgggg acgcccgcgg cgccgagcgt ggtcagggcg tcaggctggt tctccagatc
    11341 gcgtacgacg tcgacgagta actgggtctt gaggaaggag agtttccggg tggtgaagtc
    11401 gtacacgagg gcgccgaacg gttccggccg caacgccacg ccggagctga ggcggtacgg
    11461 ccggcccgct tcgaacggct cagtagacac cgcacatgcc gtcgatcgaa acttcctcga
    11521 cgagcagatc ttcctcaacg gtaacctctg tggtttctgc ttcgttcatc gcagcgcctc
    11581 ctcgtcgagt ggggtgaccc gtgaacgcga gagtaatggc acccagtgcc aaatggaagg
    11641 ggtcatggtc accagtccgc cagaactacc cgttcggccg gggaggcgtc gttccacgag
    11701 ccgacaacaa ctcgaaagtg tcgccttcga gttgttcgag ctgcacggat tcgacgcgac
    11761 caccgtcgat gacatcgcca agggcgcggg tatcgggagg cgcaccttct tccggtactt
    11821 cgaatcgaaa aacgatgtcg tgtggggcgc tttcagcgaa caactcgacc gcatgcgcct
    11881 gcaattcgac agccgtcccg ccgacgagcc gctcatggcg tcgttgcgtg cggtcgtggt
    11941 cgagttcaac agggtcgagc ccgaggagga gccgcgccac cgcaggcgca tggaactgat
    12001 cctgcgcgtt cccgccctgc tcgcgcattc aaccctccgc tacggcgaat ggcgggacgt
    12061 cgtcgccgag ttcgcggcgg gaaggctcgg cgagcggccg gaatcgctgc gcccgcaagc
    12121 catcgcccac gccgcgctcg gcgtggcgct tgccggatac cagcactggc tcggcgaagg
    12181 cggcgtcgag cgggtcgcgc tgtgcgacgt cctcgacgcc gccttcgccg acctcgcgga
    12241 agggttcgct ccgcctcccg gagaacctac gcgggaggct tgagctcgac gatctcggcg
    12301 gaagcctcac cagccggagt gcggtaggtg aacttgtcgc ccttcttgtg accggcgatg
    12361 gccttgccga gcgggctgtc ggcggtgagc gccgaaagat cgtcacccgc ttcctccgcg
    12421 atcccgacga ccaccatctc ctcgacactg ccgtcggcga acttcagcgt gacctgggtg
    12481 ccggagggca acgcggaatc ggaatccggt atcggcccac cgtgcagcag gtcggtgatt
    12541 tcggcgatcc gctggtcaag ccgtgacgcg tgctcggcgc ggccgagcaa ttcggcctga
    12601 tccgcgctgt cgccaagcgg atcgtcgccg agccgcgccg ccagcgcact ccgttgctct
    12661 cggatctgtt ccagctcgtt ctcaagccgc tggcgggtgg ccgggctcaa gcctccggtc
    12721 gacgtcatcc gcccattgtc aggcttgacc gcccggcccg ccacgtcatc gcgggtcgcg
    12781 tttcacttgc cgtatccggc cttgcgcaac gcgtcggcca tcgcaccacc agcgggggcc
    12841 ttgcggtcat cgcgctgctt tccgccctgc tgcttgccct tgcgctggcc gccctggccc
    12901 tggttgccgg acccgcgctg cccctgctgt ccgctctgtc ctcgcggtcc gccgccgcgc
    12961 tgcccctgac cgggtgaggt gtcgtcgtcg agccgcagcg tcagcgagat ccgcttcctc
    13021 ggcacgtcga catcgagcac cttcaccttg accacgtcgc ccggtttgac gacctcgcgc
    13081 gggtccttga cgaacgtgcg cgacatcgcg gagacgtgca cgaggccgtc ctggtgcacg
    13141 ccgacatcga cgaaagcacc gaacgcggcg acgttggtga cgacgccctc cagcgtcatc
    13201 ccctgcttga ggtcgcccat cgtctcgacg ccctcggcaa aggtggccgt cgtgaactcg
    13261 ggcctcgggt cgcgtcccgg cttctccagc tcggcgaaga tgtcggtgac ggtcggcagg
    13321 ccgaaggtgt cgtcggcaaa ggtcttcggg tcgagtccac gcagcgccct cggattgccg
    13381 atcagctcgg cgatgccgac cttcgtctcg tccaggatgc gatgcaccac cgggtaggac
    13441 tcggggtgca cgctggaggc gtcgagcgga tcgtcgccat ccgggatccg gaggaagccc
    13501 gcgcactgct cgaacgcctt cggcccgagc cgcgccacct ccttgagcgc ggtgcggctg
    13561 cggaacgggc cgttgccgtc gcggtgggcg acgatgttct cggcgagtcc cgtcccgatg
    13621 cccgacaccc tgctcagcag cggaaccgag gccgtgttca ggtcgacgcc gaccgcgttc
    13681 acgcagtcct cgacgacggc gtccagcgag cgcgagagct tcgactcggc gaggtcgtgc
    13741 tggtactgac cgacaccgat cgacttggga tcgatcttga ccagctcggc gagcgggtcc
    13801 tgtagccgcc tcgcgatcga caccgcgccg cgcagcgaca cgtccatgcc cggcagttcc
    13861 tgggacgcgt aggccgaggc ggaatacacc gaagcgcccg cctcggaaac gacgatcttc
    13921 gtcagcttca gctcgggacg cagcttcacc agctcgcccg cgagtttgtc ggtttccctc
    13981 gaagcggtgc cgttgccgat cgcgatcaac tgcacgttgt gctcgcgggc cagcttctcc
    14041 agcttgacga gcgactcgtc ccacttcgac tgcggcttgt gcgggaagat cgtgtcggtg
    14101 gtgacgacct tgccggtgcc gtcgacgatg gcgaccttca ccccggtgcg gtagccgggg
    14161 tcgagaccca tcgtcgcgcg ggtaccggcg ggggcggcga gcagcaggtc gcgcaggttg
    14221 gccgcgaaca cccggactgc ctcgtcctca gccgcctgac gcagccgcat ccgcaggtcg
    14281 atgccgaggt gaagcaggat cttggtccgc cacgcccagc gcaccgtgtc cgacaaccac
    14341 ttgtcggctg gcctgccctc gtcggacacc ccgaaacggg ccgcgatacg agtctcgtag
    14401 tcggcgcccg gattgtccga ccccggctcg cccgggtcgg tcgtcaggtc gagcacctct
    14461 tctttctcgc ccctgaacag ggcgaggatg cggtgcgagg gcagcttggt gaagggctcg
    14521 gcgaattcga agtagtcggc gaacttcgcg ccttcggttt ccttgccctg cctgaccttg
    14581 gaggtcaccc agccccgcgt ccacatccgc tcgcgcagct cgccgatcag gtcggcatcc
    14641 tcggcgaacc gttcgacgag gatggcgcga gcaccgtcga gcgcggcggc ggtgtcggcg
    14701 acccccttct cggcgtcgac gaacaccgcg gcggctgcct gcggttccgt cgacggatcg
    14761 gagagcagcc cgtcggcaag cggttccagc cccgcttccc tcgcgatctg ggccttggtg
    14821 cgccgcttgg gcttgtacgg caggtagatg tcttcgaggc gggccttgga gtcggccgcc
    14881 atgatgcggg cctccagcgc ctcgtccagc ttgccctgtt cgcggatcga gtcgaggatc
    14941 gtcgtacgcc gttcctcaag ctcacgcaaa tagcggaggc gttcctccag cgtgcgcagt
    15001 tgcgtgtcgt cgagagctcc ggtcacttcc ttgcggtatc gcgcgatgaa cggcacggtc
    15061 gagccgccgt cgagcaagcc gacagccgcc tgtacctgcc ccgcgccgac accgagctct
    15121 tcggcgatct tctggtcaat ggacaccatc tcagccacca gggcattgtg ccctgtgctt
    15181 ccctctgccc cgagcctgcc ctgtgaggca tcatcggctg atggccgatc gcgacaggaa
    15241 accacgcagc accgaggtca ccgacggcat cgagagggca gccgcgagag gcatgctcag
    15301 ggctctcggt atgggggacg acgatttcgc gaagccgcag atcggtatcg cgtcctcgtg
    15361 gaacgagatc accccgtgca acctgtcgct gcgcaggctc ggagacgcgg cgaagctcgg
    15421 cgtgtcaggc gcggcggggt tcccgatgga gttcgggacc atttcggtga gcgacggtat
    15481 ctcgatgggg cacgagggga tgcacttctc cctcgtgtcg agggaggtca tcgccgattc
    15541 ggtcgagacc gtgatggagg ccgaacggct cgacggcgcg gtactgctcg ccgggtgcga
    15601 caagagcctg cccggcatgc tgatggccgc cgcgcggctc gacgttgccg cggtgttcct
    15661 ctacgccggt tccaccctgc ccgggacggt cgacggcagg gaggtcacca tcatcgacgc
    15721 gttcgaagcg gtcggcgcct gcgcgcgggg gctcatcggc cgcgacgagg tcgagcgcat
    15781 cgagaaagcc atctgccccg gcgagggtgc ctgcggcggc atgtacacgg cgaacacgat
    15841 ggcgtgtgcc gcggaggcgc tcggtatgtc gttgcccggc tcggccagcc cgccttcggt
    15901 ggatcgcagg cgcgaccggt tcgcccgcgc gagcggggag gcggtcgtcg ggctcatcga
    15961 cgcgggcgtc accgcccgcg acatcctcac cagggaagcg ttcgagaacg cgatcgccgt
    16021 cgtcatggcg ctcggcggct caacgaacgc ggtgctgcac ctgctggcca tcgcccatga
    16081 ggccgacgtc gagctgactc tcggggactt cgccaggatc ggggacaggg tcccgcacct
    16141 cgccgacgtg aagcccttcg gcaggcacgt catgacggcc gtcgacaggg tcggcggggt
    16201 acccgtggtg atgaaggcgc tgctcgacgc ggggctgctg cacggcgatt gccgcaccgt
    16261 gacggggaag acggtcgccg agaatctcgc cgagctggcg ccgcccggcc tggacggcga
    16321 ggtgatccgg aagctggccg atcccattca tccgacggga gggttgacga tcctgcacgg
    16381 cagcctcgcg ccggagggtg ccgtggtgaa gagcgcgggc ttcgactcgg cgaggttcga
    16441 aggcacggca agggttttcg acggcgagca ggccgcgatg aacgcgctcg gcgagctgaa
    16501 ggccggtgac gtcgtcgtca tccgcaacga ggggccgagg ggagggcccg ggatgcggga
    16561 gatgctggcc gtcaccggcg ccatcaaagg cgccggtctc ggcaaggacg tgctgttgct
    16621 gaccgacggc cggttctcgg gcggtacgac ggggctgtgc atcgggcacg tggcgccgga
    16681 ggcggcgcac ggaggcccga tcgcgttcgt gcgtgacggt gacccgatcg tcctggacat
    16741 gacggcgcgc accctcgacg tgaaagtcgg ggagcaggag atggcgcgcc gtgccgaggg
    16801 ctggcggatg ccggaggtgc cgcgccggac cggtgtgctg gccaagtacg cgaagctggt
    16861 cggctcggcc gcgaacggtg ccgtctgcac ctgaggcccg tcgccgcccc gaacccgccg
    16921 agttctgcgt cccggacgcc gagatctgca cccaggttgc cgagttccgc gctcaggccg
    16981 ccgagatgcg cgcccggccg cccgccgacg gacggccgtc aagcacgcct ggccgttgtg
    17041 caccagcgcg gtcaattcgc ggacccggtc ggcgatgccg tcggtactcc acgacgccct
    17101 gcccgctctt ccggtgttcc cggccgcgat ctcgacgtgg atcccgagtt cccggatggc
    17161 ttcgaggtcg gccttcggtg tcaccgggcc gagctccggg ctagggcggt ccgggcgggt
    17221 cgagcgcagg aagaccgtcg atgctgtgcg cgttacgagt ggcccagtac tcggccaggt
    17281 cctcgtcgct gcgccgggag tgcctgcgta gcaacagatc ccggtcttcg gtgtagccga
    17341 ggagcgggac ggagaagccg cacgagtcgc tgaccctggt gacctcgacg tcgatgatgg
    17401 cgcgcaggcc ctggtgctcg ccggtgtcga accgggcaag cagctcgccg aacccagggg
    17461 agccggggag tacggcgcgg cctctgccgt gcaggcgcac gatgttcggc ggaccggtga
    17521 aggcacagaa catcagcacg atccggccgt tctcccgcag gtgggccagg gtctcggcgc
    17581 cgctgccggt gtagtcgagg tagcccactt cgtgctcgcc gaagacggcg aaggtgccgc
    17641 gcattccctt gggggacacg ttgatgtgtc cttcgtcgga cgtgggtgcc gtgccgacga
    17701 agaacaccgg ctggtcgaga aggaaccggc tgagccgggc gtcgatccct tcgtgcactt
    17761 tcatgcctac tcgccttcga gatcaccttc gatacggagg tatacggcgc gcatcttgtc
    17821 gagcaattcg gcgtccggtt cggcccacag cccgcggtcg acggcctcgt cgagccgttc
    17881 gatgatgccg cgcaacgccc acggattggc ctgtgcgagg aattcctggt tttccttgtc
    17941 cagtacgtag gactcggtga gctgctcgta catccagtcc tgcacgaccc ctgccgtcgc
    18001 gtcgaacccg aagaggtagt cgacggtcgc ggcgagttcg aacgctccct tgtagccgtg
    18061 cttgcgcatg gcggcgagcc acctggggtt gacgacgcga gcacggaaca cccgcgtggt
    18121 ttcctcgccg agagtgcggg tgcgcacggc gtccgggctg gtgctgtcac cgacgtagga
    18181 cgtgggcgcg gttccggtga gcgcgcgcac cgtcgcgatc atgccgccgt ggtactggaa
    18241 gtagtcgtcg gagtcggcga tgtcgtgctc gcgggtgtcg gtgttcttcg cggcgaccac
    18301 gatgcgccgg taggagctct ccatgtccgc gcgggctggt gcgccgtcga ggtcgcggcc
    18361 gtaggcgtag ccaccccata cggcgtagac ctcggcgagg tcggagtcgt ctcgccagtt
    18421 gccggaatcc atgagcggca gcagtcccgc gccgtagctg cccggtttcg agccgaagat
    18481 gcgggtggta gcccttcgtt cgtcgccgtg ggagtcgagg tcggcgcgca cgtgggcgcg
    18541 cacgaagttc gactcttccg gttcgtcgag gcccgcgacc agccggaccg cgtcgtcgag
    18601 cagatcgatg acatgaccga aggcgtcgcg gaagaagccg ctgatccgca cggtcacgtc
    18661 gatgcggggc ctgccgagtt cggcgagcgg gatggcctcg acgccggtga ccctgcgcga
    18721 agcctcgtcc cataccggcc ggacgccgag tagcgcgagc acttccgcgg cgtcgtcacc
    18781 cgaggtgcgc atcgccgagg ttccccacac cgagagaccg acggagcggg gccagtcgcc
    18841 ggtgtcctcg cggtagcggg tcagcagcga gtcggcgagc gcctgtcccg tctcccacgc
    18901 cagcctgctc ggaatcgcct tggggtcgac ggtgtagaag ttgcggccgg tcggcagcac
    18961 gttgacgagt ccgcgcagtg gcgatccgct gggccctgcc gggacgtagc caccgtcgag
    19021 cgcgtgcagt accgaggtga gctcaccgct ggttcccgcc agcctcggca cgatctcctc
    19081 ggcggcgaac cggagcacgg gtacggcggc tggagcgtcg gcgcacaccg tctcgactgc
    19141 cgcggcgttc cagccgttgt cctccatggc caccacgagg tcgcgtgccc gctgctcgat
    19201 ggcatccact tcggacagtg cggcgttctc ggtgagcccc agcgcggtgc gcagccccgc
    19261 gacggcgccc tgtttgccgc cccagatctg ctgggctcgc agcatcgcga gcacgaggtt
    19321 gacgcgtgcc tcgccggagg gggcttcgcc gaggatgtgc agcccgtcgc ggatctgcgc
    19381 gtccttcacc tcgcacaacc agccgtcgat gtgcagcagg aagtcgtcga actccgcgtc
    19441 gtgcggtcgt tgctcgacgc cgaggtcgtg gtcgagtttg gccgcctgca ccagtgtcca
    19501 gatctgctgc cggatggcgg gcagtttcgc cgggtccatg gccgcgatgt tggcgtgctc
    19561 gtcgagtagc tgttccaggc gcgcgaggtc gccgtagctt tccgctctcg ccatgggcgg
    19621 gatgaggtgg tcgacgatcg tggcgtgtgc cctgcgtttg gcctgcgcgc cctcgccggg
    19681 gtcgttgacc aggaacgggt agatcaacgg caggttgccg agcacggcgt cgggtgcgca
    19741 ggacgcggag agtcccgccg tcttgcctgg cagccattcc agcgagccgt gcttgccgag
    19801 gtgcaccacg gcgtgcgcgc cgaactcctc ttcgagccac cggtaggcgg cgagatagtg
    19861 atggctcggc ggcaggtcgg ggttgtggta gatggcgacg gggttctcgc cgaacccgcg
    19921 tggcggctgg atcatcagca ccacgttgcc cgcgcgcaaa gccgccagca cgatgtcacc
    19981 gtcaccgtcg ccggagcggt cgacgtagag gtcgccggga ggcggccccc agtgttcgac
    20041 catgtcttcc cgcaggtcgt cgggaagcgc ggcgaaccac tgccggtagc gggaggcggg
    20101 gacgcggatg gggttgccgg aaaggtgttc ctcggtgagc cattccggat cttggccgcc
    20161 cgcttcgatg agcgcgtgga tcagcgcgtc accgtcgggc tggtcggtgc ccgtcggtac
    20221 gacgccgggg aaggcgtcct cgccgaggtc gtaaccgagt tcgcgcatgg cgcgcagcag
    20281 cttgaccgcg gaggcgggag tgtcgagacc gacggcgttg cccacccggg agtgcttcgt
    20341 ggggtaggcc gagagcatca gcacgatctt gcggtcgcgg gcaggggtgt gccgcagcct
    20401 ggcgtgggcg agcgcgatcc ccgccacgcg ggaggcccgt tcggcgtcgg ggacgtagcg
    20461 ggggaggccg tcggcgtcga attccttgaa ggagaaggga accgtgatga gcctgccgtc
    20521 gaactcgggg accgccatct ggttgcccgc gtccatgggc gaaaggccct cgtcgttgcc
    20581 gagccaggtt tccctgtcac tggtcaggca cagtgcttgc agggtcggta cgtcgagcgc
    20641 ggcgagttcg gcgacgtccc acgattcgtc gtcaccgccc gcgcccgctt ccgatggccg
    20701 ggttcctccc gcggccagta cggtcaccag caacgcgtcg gccttgcgca gttcggccag
    20761 catcgccggt tcgcgggagc gaagcgaggc gcagtacacc ggcagcgcgc gcccgcccgc
    20821 cgcctcgatc tgctcggcga gggtttccac gaacgcggtg ttgccggaca gatggtgcgc
    20881 ccggtagtag aggatcgcga tggtcggccc cccggaatcg gggcgctcaa gcactcccca
    20941 cgagggctgc tcgacgggct gctcgaatcc ctcaccggtc agcagcagcg tgtcggagag
    21001 aaaccggtgg agctgggcga gattgcccgg cccgccgtgt gcgaggtagg cgtgtgcctc
    21061 ggccgcgata ccggcgggcg cggtggacag cgccatcagt tcggcgtccg gggtctgttc
    21121 accaccgagc acgatgacgt gcgcgccgct gtcgcggacc agtcgcagtc cgtcggccca
    21181 ggtgcgttcg gtgccaagga tgcggacgac gacgacggtg gcacccgtca gcaggtccgg
    21241 cagctcggcg aggtcgagcc gcgacgggtt ggccagccgg taaccggcgg cgctcgcttt
    21301 ggcactcagc aaatcggtgt ccgacgtgga cagcagcaag atcacgggaa ccctccttgc
    21361 cgttcactct ccgtgccagg ggggcacccc gtcaaggtga tccacaggga ggtcacctct
    21421 tacagtgggg gcgtgccagg gtctccgcct gttcccgatc gcgtccgcgc cgacgcgtgc
    21481 cctggcgttt
//
