LOCUS       NZ_CP017060            10276 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Bacillus cereus strain FORC_047 chromosome, complete genome.
ACCESSION   NZ_CP017060
VERSION     NZ_CP017060.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5365433)
  AUTHORS   Lee,J.-H., Kim,Y.-T., Ku,H.-J., Kim,H., Kim,H., Choi,S.-H. and
            Ryu,S.
  CONSRTM   Food-borne Pathogen Omics Research Center
  TITLE     complete genome sequence of Bacillus cereus FORC_047
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5365433)
  AUTHORS   Lee,J.-H., Kim,Y.-T., Ku,H.-J., Kim,H., Kim,H., Choi,S.-H. and
            Ryu,S.
  CONSRTM   Food-borne Pathogen Omics Research Center
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2016) Food Science and Biotechnology, Kyung Hee
            University, 1732, Deogyeong-daero, Giheung-gu, Yongin, Gyeonggi
            17104, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: PacBio SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 169
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002220285.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:13:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,922
            CDSs (total)                      :: 5,768
            Genes (coding)                    :: 5,607
            CDSs (with protein)               :: 5,607
            Genes (RNA)                       :: 154
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 73 of 161
            Pseudo Genes (incomplete)         :: 89 of 161
            Pseudo Genes (internal stop)      :: 42 of 161
            Pseudo Genes (multiple problems)  :: 36 of 161
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:30:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2614411
            Orig. end                         :: 2624687
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017060.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Food-borne Pathogen Omics
            Research Center.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10276
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10276
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10276
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5276](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5276
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            978..2339
                     /db_xref="GeneID:72449373"
                     /locus_tag="FORC47_RS13680"
                     /old_locus_tag="FORC47_2557"
     CDS             978..2339
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:72449373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017763222.1"
                     /locus_tag="FORC47_RS13680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2557"
                     /product="glycosyl hydrolase family 8"
                     /protein_id="WP_089171161.1"
                     /transl_table=11
                     /translation="MNGKRKFFTCISIVGIGIASFSNSSFAASVTDNSIQNSIPVVNQQ
                     VAAAKEMKPFPQQVNYAGVIKPNHVTQESLNASVRSYYDNWKKKYLKNDLSSLPGGYYV
                     KGEITGDADGFKPLGTSEGQGYGMIITVLMAGYDSNAQKIYDGLFKTARTFKSSQNPNL
                     MGWVVADSKKAQGHFDSATDGDLDIAYSLLLAHKQWGSNGTVNYLKEAQDMITKGIKAS
                     NVTNNNRLNLGDWDSKSSLDTRPSDWMMSHLRAFYEFTGDKTWLTVINNLYDVYTQFSN
                     KYSPNTGLISDFVVKNPPQPAPKDFLDESEYTNAYYYNASRVPLRIVMDYAMYGEKRSK
                     VISDKVSSWIQNKTNGNPSKIVDGYQLNGSNIGSYPTAVFVSPFIAASITDSDNQKWVN
                     SGWDWMKNKRESYFSDSYNLLTMLFITGNWWKPVPDDKKTQNLINDAIYEGYDN"
     gene            2502..3425
                     /db_xref="GeneID:72449374"
                     /locus_tag="FORC47_RS13685"
                     /old_locus_tag="FORC47_2558"
     CDS             2502..3425
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /db_xref="GeneID:72449374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="FORC47_RS13685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2558"
                     /product="nucleoside hydrolase"
                     /protein_id="WP_078187144.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATKPLTEEITTYYPEIHGAEGLGPIHVPENILSIPIYNF
                     GSIAAILIKYGKDLTIVDVGRSTSLAIAFNLWNDLMLNVKGIYFMGGVFLEVGNVTPLA
                     EANVYGDPIASKIVFHQAKNLFIFPLNVTNKAVLTPNVFNYIQANTKNPFHMIMKPMYD
                     YYLSAYQKLNPSMEGPLLHDVVAISGLVNPSFFEFVHRTVNVDTYGDTKGQTFADFRPS
                     SISDGAHIALEIDEQKFIQDFMKIML"
     gene            3502..3960
                     /db_xref="GeneID:72449375"
                     /locus_tag="FORC47_RS13690"
                     /old_locus_tag="FORC47_2559"
     CDS             3502..3960
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:72449375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102696.1"
                     /locus_tag="FORC47_RS13690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2559"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_001102684.1"
                     /transl_table=11
                     /translation="MNVQLKVVTRENWEEALKLQVKGNQLKFVPSVAVSLAKVYIKPDG
                     DNVEYIPFAIYDGDLMVGFVMHAVVIETSDMYWINGFIIDQSQQGNGYGKAALQESIDI
                     IKNTFKSCKEIRLTVHKDNISAKKLYERYGFISLGQEYDGEQVYRLFV"
     gene            3991..4329
                     /db_xref="GeneID:72449376"
                     /gene="cutA"
                     /locus_tag="FORC47_RS13695"
                     /old_locus_tag="FORC47_2560"
     CDS             3991..4329
                     /GO_process="GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:72449376"
                     /gene="cutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160602.1"
                     /locus_tag="FORC47_RS13695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2560"
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="WP_089171162.1"
                     /transl_table=11
                     /translation="MQFTEVKIEVFIPEEYIETLRDELNKIGACKTGEYDHCLSYSSVK
                     GYWRPLDGASPFNGEIGQICEGQECKIEIKCKRDLVKSALEVINEIHPYETPMIYIIPI
                     LNDYFEQV"
     gene            4355..4801
                     /db_xref="GeneID:72449377"
                     /locus_tag="FORC47_RS13700"
                     /old_locus_tag="FORC47_2561"
     CDS             4355..4801
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:72449377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="FORC47_RS13700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2561"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_089171163.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAVMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LKRELREELGVNCSIKRFLGVIENQWQDKEVLHHEINHIFEVESQDLHTDLTRKSSESH
                     LAFHWIDYNKEALNHYEIMPMPLVKELLERKLNDELLNCWISNF"
     gene            5001..5276
                     /db_xref="GeneID:72449378"
                     /locus_tag="FORC47_RS13705"
                     /old_locus_tag="FORC47_2562"
     CDS             5001..5276
                     /codon_start=1
                     /db_xref="GeneID:72449378"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071381.1"
                     /locus_tag="FORC47_RS13705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2562"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_157676094.1"
                     /sec_met_domain="TIGR03601 (E-value: 2.9e-43, bitscore:
                     144.5, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQSLNLNDYQTGNVVYWDQQQSQYPYFYIQDDARRCGG
                     CGGCGGRCGGCGGRCGGCAGRCGGCIGCAGCAGCFSCFNCWNWWII"
     gene            5378..6394
                     /db_xref="GeneID:72449379"
                     /locus_tag="FORC47_RS13710"
                     /old_locus_tag="FORC47_2563"
     CDS             5378..6394
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:72449379"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288887.1"
                     /locus_tag="FORC47_RS13710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2563"
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_089171165.1"
                     /transl_table=11
                     /translation="MYTYDKLISWVEDIKEKNHSSATALCIIKDNKIVLEHYSGYHSNT
                     STSKNVTASSQFNVASARKSYLGLMIAYALYEGKINSIDDEAIKYFKDFDPALLSKTTI
                     RHLVTHSHGLEETNDRTIFREFAPGQSWAYRDINVRMMTRLIYQLYNKSFPELLKERVF
                     KPANFQETGWRVQRDENLVDVVNNPNEDAISEIGTVDDGTEKNLFVSAREFAQWGNLHL
                     NQGGIDGKQIVPKEVIKIATSLQSPTYINKELPQNGLFWFIQNEPAQLSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSNGNRA"
     gene            6426..6569
                     /db_xref="GeneID:72452591"
                     /locus_tag="FORC47_RS30890"
                     /old_locus_tag="FORC47_2564"
     CDS             6426..6569
                     /codon_start=1
                     /db_xref="GeneID:72452591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="FORC47_RS30890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2564"
                     /product="hypothetical protein"
                     /protein_id="WP_071732166.1"
                     /transl_table=11
                     /translation="MKDKVNKDKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFDEI
                     EY"
     gene            6849..8057
                     /db_xref="GeneID:72449380"
                     /locus_tag="FORC47_RS13720"
                     /old_locus_tag="FORC47_2565"
     CDS             6849..8057
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:72449380"
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="FORC47_RS13720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2565"
                     /product="OFA family MFS transporter"
                     /protein_id="WP_089171166.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAAKLQKKLGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLIDNLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNAVTETLHNDYTPREMMRTKQVYLLFFMLFTS
                     CMGGLYLISMVKDIGVQLVGLSAATAANAVAMIAIFNTVGRIILGTLSDKIGRMKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGAILGGFQPTFIIIGVLSVISFIISILIRPPNVEKKKELKHLHR
                     KIA"
     gene            8188..8613
                     /db_xref="GeneID:72449381"
                     /locus_tag="FORC47_RS13725"
                     /old_locus_tag="FORC47_2566"
     CDS             8188..8613
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:72449381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706722.1"
                     /locus_tag="FORC47_RS13725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2566"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_089171167.1"
                     /transl_table=11
                     /translation="MKIVQQWVQEDSDYIREKVIEYNQKYLADEEKTPSEKVSFIVRNE
                     KEEIVGGVTAITFWHHIHVDFLWVSEEYRHEGYGSKLIKLIEEFAIEKECRLINLDTFS
                     FQAPDFYKKHGYKVIGVSKDHPKGHNHYYLEKRLKSI"
     misc_feature    complement(8794..8812)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 22.4"
                     /tool="antismash"
     gene            8893..9336
                     /db_xref="GeneID:72449382"
                     /locus_tag="FORC47_RS13730"
                     /old_locus_tag="FORC47_2567"
     CDS             8893..9336
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:72449382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="FORC47_RS13730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2567"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_029442479.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDKTNEKILMVKNKGENGSYYTLPGGAVKLGETL
                     EEAVIREVKEETGLHINVNGICSISEAFFEERDHHAIFFNFLGEIIGGETYISRPKEIE
                     EITWMELHIAAPYLRIPEHLLDLLQRKETVPYFFNGTIVHQSS"
     misc_feature    10123..10141
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.64"
                     /tool="antismash"
ORIGIN
        1 cagcaattcg tatggggctt gaatcttata cgcctgaaca ccttacttta ctacgcttat
       61 taatcgcctc atttatactt ttgttatttt catttatata caaactacga ctaccggatt
      121 taaaggatat cccagcaatt tttatatttg gtgctctagg atttactatt tatcacatcg
      181 cattaaacta cggcgaaaaa acagtaaatg ctggatctgc aagtttaata atttcagtca
      241 ctccaatagt aacggcaatt cttgcttctg tttttttgaa tgaaaaaatg aaattaaatg
      301 gctggatagg tggtctaatg agttttgcag gaattgcctt aatttcattt agccaaggcg
      361 acgctattca attgaatagc gggggattat ttatattatt agcggcgatt tcagaaagtc
      421 tatttttcgt tttccaaaca tcttacttaa aaaaatacgg cttcttacca tttaccatat
      481 atacaatttt gtctagtact gtatgtatgc ttattttctt acccggagtt tatcaaaaaa
      541 tactagcagc tcctcttgaa gttaacttga gcgttatata tttaggtgtc tttccaacag
      601 tactcccgta tattgcattg gcctatatta tatctcgtgc tggtgcttct gaagcaacaa
      661 gttctctata tttaacgcca ataactgcgt gtttcattgc ttggatatgg ttaggagaag
      721 tgccaacttt agtttcgata attggcggag gaattactat acttggaatt gtgattgctc
      781 atataccagt attaagaaaa gaaaaacata ggaatttagc aaataatcaa tccgtataat
      841 gtaattcaat aatgatctta tgatattttt tcatatagtt tctttcttat gttgataaat
      901 ataatatttt gtatttcagt taaatattgt atgttacatt ttatgtagta agcattttaa
      961 aaggagctga caacataatg aatggaaaaa gaaaattttt cacatgtatt tctattgtag
     1021 gaatcggaat agctagtttt tctaattcta gtttcgcagc aagtgtaacg gacaattcaa
     1081 tacaaaattc tattcccgta gttaatcaac aagtagctgc tgcaaaggaa atgaaaccat
     1141 ttccccagca agttaattat gcaggtgtta taaaaccgaa tcatgttaca caggaaagtt
     1201 taaatgcttc tgtaagaagt tactacgata attggaaaaa gaaatatttg aaaaatgatt
     1261 tatcttcttt acctggtggt tattacgtaa aaggagagat tacaggtgat gcggatgggt
     1321 ttaagccact tggaacttca gaaggtcaag ggtatgggat gataattaca gtattaatgg
     1381 ctggttatga ttcgaatgct caaaaaatct atgacggttt atttaaaaca gcaagaactt
     1441 ttaaaagctc tcaaaatcct aatttaatgg gatgggttgt cgcagatagt aaaaaagcac
     1501 aaggtcattt tgattctgct actgatggag atttagatat tgcgtattct cttcttcttg
     1561 ctcataagca gtggggatct aatggaacag ttaattattt gaaagaagca caagacatga
     1621 ttacaaaagg tattaaagct agtaatgtta caaataataa ccgactaaat ttaggagatt
     1681 gggattctaa aagttcactt gatacgagac catctgattg gatgatgtca caccttagag
     1741 cattttatga atttacaggt gataaaactt ggcttactgt tattaataat ttgtacgatg
     1801 tttatacgca atttagtaat aagtactctc caaatacagg acttatttca gatttcgttg
     1861 taaaaaaccc accacaaccc gcacctaaag acttcttaga tgagtcagaa tatacaaatg
     1921 cctattatta caatgctagt cgggtaccat tgagaattgt aatggactat gcgatgtacg
     1981 gtgagaaaag aagtaaagtc atttctgata aagtctcttc atggattcaa aataaaacga
     2041 atggaaatcc ttctaaaatt gtggatggtt atcaattaaa cggatccaat attgggagtt
     2101 atccaactgc tgtattcgtt tcaccattta ttgctgcaag tataacggat agcgataatc
     2161 aaaagtgggt aaatagcggt tgggattgga tgaagaataa gagagaaagt tattttagcg
     2221 atagttataa tttattaact atgttattta ttacaggaaa ctggtggaag cctgtacctg
     2281 atgataaaaa aacacaaaat ctaataaatg atgcaattta tgaaggatac gataattaat
     2341 taaatttgag aatggttact gaagaaagga attagcattc gctagttcct ttttaatgtt
     2401 ttgtaaaaca ttatccattc taaaattcgc atagatatac ttgctttttt catatacata
     2461 gttattgatg actctatgtt tatatgaagg gagagtattc cttggaaaaa gtattatttt
     2521 tcggagaccc aggtattgat gactcatttg ccattatgta tggtttactg catcccgaaa
     2581 ttgaaattgt cggtattgta accggatacg gaaatgttga gcatatacat gctgcacata
     2641 atgcagcgta tattttacag ttagcaaata gacaagacat tcctgtcatt agtggtgcaa
     2701 caaaaccact tacagaagaa attactacgt actatcctga aattcacgga gcagaaggat
     2761 tagggcccat ccatgttcca gaaaatatat tatctattcc aatatataat ttcggcagta
     2821 ttgctgcgat tcttataaag tacggaaaag atttaacgat tgttgatgta ggaagatcta
     2881 cctcgttagc aattgctttt aatttatgga atgatttaat gctaaatgta aaaggaatct
     2941 atttcatggg tggtgttttt ttagaagtag gaaacgttac gccattagca gaggcaaacg
     3001 tgtatggaga tccgatagca agtaaaattg ttttccatca agcaaagaat ttattcatat
     3061 tccctttaaa tgtaacaaac aaagctgtgt taacaccaaa tgtatttaac tatattcaag
     3121 ctaatacaaa gaatccattt catatgataa tgaaaccaat gtatgattac tatctatccg
     3181 cataccaaaa acttaatcca tctatggaag ggcctttgct acatgatgtg gttgcaataa
     3241 gtggattagt aaatccaagt ttttttgaat ttgtgcatcg tacagtaaat gtagatacat
     3301 acggagatac gaaaggacaa acatttgctg attttcgccc tagctctata tctgatggag
     3361 cccatatagc actagaaata gatgaacaaa aattcattca agattttatg aaaattatgt
     3421 tataagttgt tgtcgaaaaa gggaattatt tcactacgtt gaatataaac atacatcctc
     3481 tatataaggg agcttacaat attgaatgtc cagttaaagg ttgttacgag agaaaattgg
     3541 gaagaagcat taaagctaca agtaaaagga aatcagttga aatttgttcc atccgtagca
     3601 gtttcgcttg ctaaagtata tataaaacca gatggtgaca atgtagagta cataccattt
     3661 gctatatacg atggtgacct tatggttggt tttgttatgc atgctgttgt aatagagaca
     3721 tcggatatgt attggattaa cggatttatt attgatcaat cgcagcaagg taatggttat
     3781 ggaaaagcag cattacaaga aagtattgat ataataaaaa atacttttaa atcgtgtaaa
     3841 gaaataagat taaccgtaca taaagataat atctctgcaa agaaattata tgaacgatat
     3901 ggtttcatat cattaggaca ggagtatgat ggtgagcaag tataccgttt atttgtttaa
     3961 ctattaagaa gtaaaggtgt gaaatgaaat atgcaattta ctgaagtgaa aattgaagtg
     4021 ttcattcctg aagaatatat tgaaacatta agggatgaat taaataaaat tggagcatgt
     4081 aaaacgggcg aatatgacca ttgtctttcc tatagttcag tgaaaggata ttggagaccg
     4141 ttagacggcg catccccttt taatggggaa attggtcaaa tatgcgaagg gcaagaatgt
     4201 aaaattgaaa taaagtgtaa acgagatctt gtaaaaagtg ctcttgaagt tattaatgaa
     4261 attcatccat atgaaacacc aatgatttat ataatcccga tattaaatga ttattttgaa
     4321 caagtgtaga ttgaatgact aggaggaata tcaattgaaa agtaaattcc atcatattgt
     4381 acgagcagtc atgataaaag atgaaaaatt attagttgct gaatatatcg gccatcatta
     4441 ttttttacct ggtggccatg ttgaaattgg agaatcagca gagaatgcat taaaaagaga
     4501 attaagagaa gaacttggag taaactgcag tataaaacga tttttaggag ttatagaaaa
     4561 tcaatggcaa gataaagaag tgcttcatca tgaaattaat cacatttttg aagtagagtc
     4621 acaagattta catacggact taacacggaa atctagcgag tctcatttag catttcactg
     4681 gatagattat aataaagaag ctttaaatca ttatgaaatc atgccaatgc ctttagtaaa
     4741 ggaattacta gaaagaaagt taaatgatga actactaaat tgttggatta gtaattttta
     4801 aatcacttta tttatagtaa atacgtccat cgaatgaata taaagaaaat aggctgaaaa
     4861 acaaggtaca tcacctagtg taagttcata ttttgttgat gtacgcattt agtctatagt
     4921 cttgtttcta ctttgtttaa aagaatttat taactttaat atttggacgg aatttacatt
     4981 tataaatgga gggtttcata atgaatcagt ttcaacaaga actacaatca ttaaacctta
     5041 atgattatca aactggcaat gttgtgtatt gggatcaaca acaaagtcaa tatccatact
     5101 tttacattca agacgatgca cgtcgttgcg gcggatgtgg aggttgtggt ggacgttgcg
     5161 gcggatgcgg cggtagatgt ggaggttgtg caggtcgttg cggcggatgc ataggatgcg
     5221 cagggtgcgc agggtgtttc agttgcttta attgctggaa ctggtggatt atttaagtgt
     5281 agaaaatcca agctatttta tatgaaagaa aggtgtaaaa actattttgc acttttcttt
     5341 catgctgtaa ttttgatgtg tattaagggg gatttatttg tatacatatg ataagttgat
     5401 ttcttgggta gaggatataa aagaaaaaaa tcatagttct gctacagcac tttgtattat
     5461 aaaagataat aaaatcgtac tagagcatta tagtggttat cactcaaata catctacaag
     5521 caagaatgta acagcatctt cacaatttaa cgttgcttct gctagaaaaa gttatttagg
     5581 attaatgata gcgtatgcgc tttatgaggg gaaaataaac tctattgatg atgaagcgat
     5641 aaaatatttt aaagactttg atcctgcatt gcttagtaaa acgacgataa gacatttagt
     5701 aacacattcg cacggattag aagaaacgaa tgacaggaca atttttcgtg aatttgcacc
     5761 gggacaatca tgggcgtata gagatattaa tgtaagaatg atgacacgtc ttatttatca
     5821 gctatataac aaaagttttc ctgaattgtt aaaggagcgt gtgtttaaac ctgctaattt
     5881 tcaagaaaca ggttggagag ttcagcgaga tgaaaattta gttgacgttg ttaataatcc
     5941 aaatgaagac gcaattagtg aaattggtac ggtagatgac ggtactgaaa aaaatttgtt
     6001 tgtctcagct agagaatttg cgcagtgggg caatcttcat ttaaatcaag gtgggataga
     6061 tggtaaacaa attgttccaa aagaagttat aaaaatcgct acgagtttgc agagtccaac
     6121 atatataaac aaagagctac cacaaaatgg attgttttgg ttcatccaaa atgagcctgc
     6181 gcaattaagc gaacttggtg aacgtgttcc aaaagggtcg tatcaaatat tagggattac
     6241 tggaccgacc attttagtaa tacctgaata taatgtagtt gttgcaaaaa tgtataataa
     6301 aagatataac tacggtggtg ataattactt atattattta cgtgaattta gtaatttagt
     6361 cgcagataca tttagtaacg gtaatagggc ataattactc ctataacgaa aaggagtgat
     6421 tgaaaatgaa agataaagtg aacaaagata aaaagcaaaa tataaaagat gtaaatacag
     6481 agcaaaatgc aatttatagc gatcctaaag atgcagctaa tatgcaaact gtaccgcagc
     6541 cgaaagattt tgatgaaatc gaatattaat ttttcatccg actatatgat gaatgaaaaa
     6601 tacgtcttaa gtctcataac atttaagaaa ttatttgata acatatatac ataagcattt
     6661 gaaaatgtct tatcttaaca aagctttctt tagaaatatc tgatataacc tgcaaaaggc
     6721 acttctttat gaagtgtctt tttgtgtata tgaacttaat taccattttc cgtacagtat
     6781 cttgcaattt atgatggata tactaactgt gaaaattcat gtcaagataa aagaagggga
     6841 cagttagtat gaaacaaact tctataaatc cgttactaat tgttctaggt acaatcattg
     6901 ttcaaattgg ccttggaaca atttatacat ggagtttatt taatcaaccc cttgtaagta
     6961 agtttggatg gaatcttaat tcagtagcga taactttctc cataacaagt ttttctttat
     7021 cattctcaac tttatttgca gccaagttgc agaaaaaatt aggacttcga aaacttattg
     7081 ctactgcagg aattgtttta ggactcggct taatacttag ttcacaagtt tcttctttac
     7141 cactattata tttattagct ggtgtcgttg ttggttatgc ggatggaaca gcttatatta
     7201 catcactatc taatttaatt aaatggtttc caaatcggaa agggcttatt tcaggtatat
     7261 ctgtatcagc gtatggaatg ggcagcttaa tctttagata tataaacgga aatcttatcg
     7321 ataaccttgg tgtatcacaa gcattcttat attggggtat tatcgtgtta cttttagtgt
     7381 taattggatc cttcttctta cgtgaggcaa ttgtaagtaa tgctgtaact gaaacattac
     7441 acaatgacta tactccgcgt gaaatgatgc gaacgaaaca agtatatctg ctatttttta
     7501 tgttattcac ctcgtgtatg ggtggtttgt atttaatcag tatggtaaaa gatatcggcg
     7561 tacaacttgt tggacttagc gcagcaaccg ctgcaaacgc cgttgctatg attgcaatct
     7621 ttaatacagt aggtagaatc attcttggga cgttatcaga taaaatcggg cgaatgaaaa
     7681 ttgtctctgc aacgtttatt attataggat tgtcagtctt tactttaagt tttattccgc
     7741 taaattacgg aatctatttt gcttgtgtag caagtgtcgc cttttgcttc ggcggtaata
     7801 taactatatt cccagctatt gtcggagatt tctttggatt aaaaaaccat agtacaaatt
     7861 acgggattgt ctaccaaggt ttcggatttg gtgcgcttgc aggatcattc attggagcga
     7921 tacttggagg atttcaacca actttcatta taattggtgt tttaagtgtt atttccttca
     7981 ttatttcaat attaatccgt ccaccaaatg tagaaaagaa aaaggaacta aaacatttac
     8041 atcggaaaat agcttaattt taaaaagagt cctcatacta gggctctttt ttattccatt
     8101 aactttgctt ctttaccaac tcattaaaga caggacaata tcattttaaa attgaataga
     8161 taagtaaaaa ggagcgtgac acttaaaatg aaaatagtac agcagtgggt acaagaagat
     8221 agtgattaca taagagagaa agtaattgaa tataatcaaa aatatctcgc agatgaagaa
     8281 aaaacacctt cagaaaaggt tagtttcata gtaagaaatg aaaaagaaga aatagtaggt
     8341 ggggtaacag caataacttt ttggcaccat atacacgttg actttctatg ggtttctgaa
     8401 gagtataggc atgaaggtta cggaagtaag ttaataaagc ttattgaaga gtttgcaatt
     8461 gaaaaagagt gcaggttaat caatttagat acttttagtt ttcaggcacc tgatttttat
     8521 aagaagcatg gatataaagt aatcggagta agtaaagatc atccgaaagg acataatcat
     8581 tattatttag aaaaacggct aaaaagtata tagaatttgt taacattagt atttggaata
     8641 cacatgcaat aagggcgagg ttattattaa tttcagtatt aattgaatca atttttattc
     8701 taaaactgca agaacgtact ttggataatc ttcctaaggt acgttctttt tttatatcag
     8761 tagcctattc ttattaattc caccaatact ggtaaaggca agacttaact aacttatata
     8821 tattaaaagg agttaatggt aaaatgaata gaagacagaa aattcctttt attaaaaata
     8881 gaggaggtac atatgaaaaa agtaaatgtt acatatgcac ttttatacga taaaacaaac
     8941 gaaaagatct taatggtaaa aaacaaaggg gaaaatggtt cttattatac attaccaggt
     9001 ggcgcagtta aattgggaga aacgttagaa gaagcagtaa ttcgagaagt aaaagaagaa
     9061 acagggttac atataaatgt aaacgggatt tgttctatca gcgaagcctt ttttgaggaa
     9121 agagatcatc atgcaatttt ctttaatttt ctaggtgaaa taattggcgg agagacatat
     9181 atatcaaggc caaaagaaat tgaagagatt acttggatgg aattacatat agcagctcca
     9241 tatttacgca taccagaaca tttactagat ttattacaaa ggaaagaaac agtaccgtac
     9301 ttttttaacg gaacaatcgt tcatcaatct tcataaaaat attatctcat gtaaggtggg
     9361 gaaaacatgg agttcatcgt taatcacaaa caatttactc aagcactttc agaagttaac
     9421 aaagcgatat caacaaaatc tttaattcct atattaactg gtataaaaat aactgcagat
     9481 caatctggaa ttactttaat tgcaagtaac tcgaatattt ttatcgaaaa attcattcct
     9541 gtttcaattg aagacgaaaa gattgcaact attataaagg caggaactat tgttgtacct
     9601 gcaaagtatt tcattgaaat aataaagaaa atgccaagtg atatagcaat aaaaagtaag
     9661 aatgagcaat taattacgat acaatcagaa gaaatcacat taagtttaaa tggatttcct
     9721 gcagatgagt ttccgaatgt accgcttata gacaatcatg cagaaatcaa agtagagaca
     9781 gagcaattga ttgaagtatt taaacaaaca gtctttgcgg tagcgaaaaa tgaatctaga
     9841 cctgttctta ctggggtgca tatagagtta tctaataata agttaatttg tgctgcaact
     9901 gactcacata gactagcgat acgtgaaaca ttgctttcct cagatgtaaa agcaaattgt
     9961 attgtaccaa gcgcaaccat tagtgaactt ctaaaattaa tgaacagcaa ttcagaattc
    10021 gtatatatct atctttcaga gagtcatatt atatttacgc tcggaacaac tacattatat
    10081 tcaagactaa tcgaagggaa atatcctaat atttccaacc ttattccgaa cgactttaaa
    10141 acgattatta acatagatag aaaaaagata ttacaaggtg tagaccgatc aagtttatta
    10201 gcaagtgaat gggccaataa caatgttaac ttagaaatca taaacgaatc cacaattaaa
    10261 atttcttcaa acgctt
//
