LOCUS       NZ_CP017060            10330 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Bacillus cereus strain FORC_047 chromosome, complete genome.
ACCESSION   NZ_CP017060
VERSION     NZ_CP017060.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5365433)
  AUTHORS   Lee,J.-H., Kim,Y.-T., Ku,H.-J., Kim,H., Kim,H., Choi,S.-H. and
            Ryu,S.
  CONSRTM   Food-borne Pathogen Omics Research Center
  TITLE     complete genome sequence of Bacillus cereus FORC_047
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5365433)
  AUTHORS   Lee,J.-H., Kim,Y.-T., Ku,H.-J., Kim,H., Kim,H., Choi,S.-H. and
            Ryu,S.
  CONSRTM   Food-borne Pathogen Omics Research Center
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2016) Food Science and Biotechnology, Kyung Hee
            University, 1732, Deogyeong-daero, Giheung-gu, Yongin, Gyeonggi
            17104, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: PacBio SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 169
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002220285.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:13:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,922
            CDSs (total)                      :: 5,768
            Genes (coding)                    :: 5,607
            CDSs (with protein)               :: 5,607
            Genes (RNA)                       :: 154
            rRNAs                             :: 14, 14, 14 (5S, 16S, 23S)
            complete rRNAs                    :: 14, 14, 14 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 73 of 161
            Pseudo Genes (incomplete)         :: 89 of 161
            Pseudo Genes (internal stop)      :: 42 of 161
            Pseudo Genes (multiple problems)  :: 36 of 161
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:30:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2501271
            Orig. end                         :: 2511601
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017060.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Food-borne Pathogen Omics
            Research Center.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10330
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="7"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10330
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10330
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5330](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5330
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    335..349
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 17.35"
                     /tool="antismash"
     gene            425..1873
                     /db_xref="GeneID:72449253"
                     /locus_tag="FORC47_RS13010"
                     /old_locus_tag="FORC47_2425"
     CDS             425..1873
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-469). E-value:
                     7.2e-30. Score: 96.2. Matches aSDomain:
                     nrpspksdomains_FORC47_RS13010_Aminotran_1_2.1"
                     /codon_start=1
                     /db_xref="GeneID:72449253"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="FORC47_RS13010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2425"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_089171086.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 7e-28, bitscore:
                     96.1, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYLKKMIMKGHLLGDQKIPSTRELSEL
                     LSVSRNTVLAAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKNKLNTVTALADE
                     LDLMKHGVRWEKGMIVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYRPL
                     MNYLLHYMEMKGVDISNKDILITNGFTEGLDIVLSSLSKKSGRVICENPTHHAALKLFR
                     LHGLEVHGIDMHEDGIDTNEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYKIPIIEDGFNEELRYSGSHLAPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQY
                     IPFKRMTGDGGLHLFIELEEHMNARTLLQKCYEKGVTFSPGDVFYSDGEGANTFRLGFS
                     RLKEEEIVRGIKIIGDTLKNEIWS"
     aSDomain        860..1831
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FORC47_RS13010_Aminotran_1_2.1"
                     /evalue="7.20E-30"
                     /label="FORC47_RS13010_Aminotran_1_2.1"
                     /locus_tag="FORC47_RS13010"
                     /protein_end="469"
                     /protein_start="145"
                     /score="96.2"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYRPLMNYLLHYMEMKGVDISNKDILITNGFT
                     EGLDIVLSSLSKKSGRVICENPTHHAALKLFRLHGLEVHGIDMHEDGIDTNEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYKIPIIEDGFNEELRYSGSHLAPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQYIPFKRMTGDGGLHLFIELEEHMNARTL
                     LQKCYEKGVTFSPGDVFYSDGEGANTFRLGFSRLKEEEIVRGI"
     gene            1878..2792
                     /db_xref="GeneID:72449254"
                     /locus_tag="FORC47_RS13015"
                     /old_locus_tag="FORC47_2426"
     CDS             1878..2792
                     /EC_number="6.3.2.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:72449254"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="FORC47_RS13015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2426"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_061660682.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 8.3e-22,
                     bitscore: 76.0, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 1.2e-13, bitscore:
                     49.7, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 5e-09, bitscore: 34.3,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 3.6e-53,
                     bitscore: 178.4, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 1.2e-05, bitscore:
                     23.2, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRY
                     EGVETPDWIELTKMEDLNLDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVF
                     EWDSEVVIEKYIKGDEITCSILDGKQLPIVSIRHAAEFFDYNAKYDDTSTVEEVIELPA
                     EINERVNKASLTCYKALKCSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAG
                     ISYSKLLDMIIETSLKVRNEEGF"
     misc_feature    complement(2815..2827)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     regulatory      2866..2971
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            3099..3788
                     /db_xref="GeneID:72449255"
                     /gene="tenA"
                     /locus_tag="FORC47_RS13020"
                     /old_locus_tag="FORC47_2427"
     CDS             3099..3788
                     /EC_number="3.5.99.2"
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:72449255"
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="FORC47_RS13020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2427"
                     /product="thiaminase II"
                     /protein_id="WP_075719332.1"
                     /transl_table=11
                     /translation="MTFSQSLRKEVDSIWEASFNHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITKEEKDNFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWISAY
                     GSDWFRTLVEEQITRLDTIAEKVTEVDRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDT
                     DVKDVIG"
     gene            4189..4452
                     /db_xref="GeneID:72449256"
                     /locus_tag="FORC47_RS13030"
                     /old_locus_tag="FORC47_2428"
     CDS             4189..4452
                     /codon_start=1
                     /db_xref="GeneID:72449256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101034.1"
                     /locus_tag="FORC47_RS13030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2428"
                     /product="DUF3937 family protein"
                     /protein_id="WP_089171087.1"
                     /transl_table=11
                     /translation="MNFERCYMFTNKKLIRFGLSLFVFLGIINFTISYFQTYLETAADI
                     KWVIPEIWKTILLDVPQGILVLLGAIALYDFTKEVSQKDASI"
     gene            5001..5330
                     /db_xref="GeneID:72449257"
                     /locus_tag="FORC47_RS13035"
                     /old_locus_tag="FORC47_2429"
     CDS             5001..5330
                     /codon_start=1
                     /db_xref="GeneID:72449257"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071363.1"
                     /locus_tag="FORC47_RS13035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2429"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_048564718.1"
                     /sec_met_domain="TIGR03601 (E-value: 8.2e-39, bitscore:
                     130.2, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQALSLNDYRSGNIVYWDQQNQYPYYYIEDAARRCGGC
                     GRCGGCGGGRCGGFRCGGFRCIGCFGCFGCGGCGGCGGCGGCSNCFDGFSGTTDTTGTI
                     ITYEY"
     gene            <5470..5604
                     /db_xref="GeneID:72452538"
                     /locus_tag="FORC47_RS30585"
                     /pseudo=""
     CDS             <5470..5604
                     /codon_start=1
                     /db_xref="GeneID:72452538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016077325.1"
                     /locus_tag="FORC47_RS30585"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="site-specific integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="YMYVPTKNIAIINGYIQSFK"
     misc_feature    complement(5764..5782)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.87"
                     /tool="antismash"
     gene            5814..6299
                     /db_xref="GeneID:72449258"
                     /locus_tag="FORC47_RS13040"
                     /old_locus_tag="FORC47_2430"
     CDS             5814..6299
                     /codon_start=1
                     /db_xref="GeneID:72449258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124614.1"
                     /locus_tag="FORC47_RS13040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2430"
                     /product="hypothetical protein"
                     /protein_id="WP_002041181.1"
                     /transl_table=11
                     /translation="MNMIELKSIIHSYKLKRKIAKDLYGKRDELTLLLNEFNKMKCTVT
                     SEKKKNNILSRLQLIYQNMKLDKQYPLSVAFDSKLLERLEKEPLHTIEDGVTCLHLMLD
                     VNYEKIKQYGSSTSRSFVPLSQSSICLADCICLTGVVVGLLGAISFGGLLLSICSIT"
     misc_feature    6606..6619
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.3"
                     /tool="antismash"
     gene            6611..7312
                     /db_xref="GeneID:72449259"
                     /locus_tag="FORC47_RS13045"
                     /old_locus_tag="FORC47_2431"
     CDS             6611..7312
                     /codon_start=1
                     /db_xref="GeneID:72449259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736205.1"
                     /locus_tag="FORC47_RS13045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2431"
                     /product="DUF3962 domain-containing protein"
                     /protein_id="WP_089171088.1"
                     /transl_table=11
                     /translation="MKKLRLLTFENIVEPLLNEKVSFIYFPIEWLDIVEIHYKTFLLTS
                     KLKRLNERLYDMFSDILFIQHNPYVLNENTPWIVSKEPIRQEQLDYIFQSWYEIIHDWK
                     PNRLVEPPKYEWQSDLISNLPVLHDIETYSKWVPALISHIFCERPIYLENTNEEEIYFS
                     PLRSQNICEAMSGPIKDEKTQDFFSYVYRFECITRGGENAPLLNISIGIRRFYQEYKMI
                     GQTNLDMTTFV"
     gene            complement(7351..8460)
                     /db_xref="GeneID:72449260"
                     /locus_tag="FORC47_RS13050"
                     /old_locus_tag="FORC47_2432"
     CDS             complement(7351..8460)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:72449260"
                     /gene_functions="other (smcogs) SMCOG1077: phage integrase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675870.1"
                     /locus_tag="FORC47_RS13050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2432"
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_089171089.1"
                     /transl_table=11
                     /translation="MKGHIRKRGNKYCIVIDIGPDPETGKRRQKWFSGYKTKKEAQADV
                     AKKITELNEGTFIEPSKVTLKDYLNHWLEIKSMSIEKSTFAGYRAFINLHVLPSIGMVA
                     LHKLNVMHIQKCYKTAIDKGIANNSILLMHRILKSALNLAIKQNIISRNPADFAEIPKK
                     EKTPIQTWTEEEVKKFLAHSQESRYHIGYLLAITTGMRLGEVLGLRWQDIDFEKHTVTI
                     NQTSGHDNKIKKTAKTNSSKRTIPVPNETIAALKKHKILINKEKLKFGSAYLDQDLINC
                     NEFGRIIKRAHFRKSFIRTTHKVGIKEIKFHDLRHTHATLLLKQGVNPKIISERLGHTD
                     ISMTLSVYSHVLPNMQEEAVKNFGKSIFG"
     gene            9006..10157
                     /db_xref="GeneID:72449261"
                     /locus_tag="FORC47_RS13055"
                     /old_locus_tag="FORC47_2434"
     CDS             9006..10157
                     /codon_start=1
                     /db_xref="GeneID:72449261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265310.1"
                     /locus_tag="FORC47_RS13055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FORC47_2434"
                     /product="AimR family lysis-lysogeny pheromone receptor"
                     /protein_id="WP_089171090.1"
                     /transl_table=11
                     /translation="MWKALNQIEKELCAAGIRKNKLANYWGVKPSTVTKVFKGNTDMSF
                     GFLSKTVILLNKGIQVQENLLTDYIHITKPKSENLREAMEDLALRGKFNLLINIINSES
                     QSKVAENREFANVYRIIYRRYIGEIDAIQYHEALSLESKSIRTSEMEVLIEILLCQAQY
                     QSGNFTALNERLKSLEIKINKISNRYIRECYKLRYKEAIAVTSLQGGEVIEARHVSMEL
                     LDDLEWDNFFSFPKVNAYLKLGESYIFSANEYEKAKYYLEKTLEVLGDSKINGIERKRK
                     MVQHTLSFLKIHHDKEISSVDVVHPGELAYLRIKQGKRIEARKLLIQLKEKNGRLTDIQ
                     TAYLGLTYEGTKKEELMKRSLLMCQKSGNIFYSNLPKIHLGLI"
ORIGIN
        1 taatggaaac agagaaaaca cttactgata tacatataaa aggaattgag cacgttacaa
       61 aacagcaaat acaaaacgct aaggaacaac ataaaattat taaattaata gcatcaatat
      121 ataaggatga gggtggcaat gtgaatttaa gtgttgaacc gtgcgaaata gaaaaagatc
      181 atccgttagc aaatgtaaat gggaccgaaa aaggaattac attctttaca gatacgatgg
      241 gacaagttac tacaattggt ggggcttcta atccacgagg cgctgcagct gcagctttaa
      301 aagatgtaat taacttatat cgaaaagatt tgtaagaccg taccgccctt tataaaaaat
      361 acatatgtcg tggacctttt gagaatgaaa taaatatgca aaaatgattg cagggggtaa
      421 ggaattgttt aaggatttta aagttgttaa agatcgtccc gtttatattc aattaaaaga
      481 ctatttgaaa aagatgatta tgaaagggca tttgctcggg gatcaaaaaa tcccatcaac
      541 aagggaacta agtgaattac taagtgtgag tagaaacact gtactcgctg cttatgcaga
      601 tttagagcaa gaaggactca tttatgcagt taaaggaaaa gggaactttg ttgcgaaggt
      661 ggatatatct aacacttcgt ctgttgaaat agattggaaa aataaactta atacagtgac
      721 cgcattagcg gacgaattag atttaatgaa acatggtgtt cgttgggaaa agggaatgat
      781 tgtttttaat agtatagccc cggatgagaa gctatttgat gttgaaaatt tcaaaagagc
      841 ttttcttact cgtatgtcca ttgaagggga catcgtattg aactacggat atgcaaaggg
      901 atatagaccg ttaatgaatt atttacttca ttacatggaa atgaaaggtg tagacatttc
      961 gaataaagat attctaatta caaatggatt tacagaagga ttggatattg tactttcttc
     1021 tttatcaaaa aaatccggtc gtgtcatttg tgagaatcca actcaccatg ctgcacttaa
     1081 gcttttccgt ttacatggac ttgaagttca tgggattgat atgcatgagg atggtattga
     1141 tacgaatgaa gttgaaaaaa gtttgcgtga aaaagatttt gattttgctt atttaattcc
     1201 ttcttatcat aatccaaccg gtattgttac gagttcagag aaaagaacgg aattgatgag
     1261 gttattctca aaatacaaga ttcctattat agaggacgga tttaatgagg aattacgtta
     1321 ttcaggttca catttagctc cattattaac ttttgcggga gctggcaata atgtgattta
     1381 tattagtagc ttttcaaagg tgcttttccc tggtttacgt gtaggctgga ttattgcaga
     1441 taaagagctg attcattatt tagaaagcgt aaaaagagca agaacgattc acacatctac
     1501 gctagatcaa gctgttctgt ttcaatattt acatgaggga tattttgaaa aatatttaaa
     1561 aaaggcaaga tctgtttata agaaaaaata tgagttagct gtcggggcat gtaaccagta
     1621 cattcctttt aaaagaatga ctggagatgg tggacttcat ttatttatag agctagaaga
     1681 gcatatgaat gcccgcacac ttttacaaaa gtgttatgag aaaggcgtta cgttttcacc
     1741 tggagatgtt ttttactctg atggagaagg agcaaacact ttccgattag ggttttcgcg
     1801 cttgaaagaa gaagaaatag ttcggggaat taaaataatt ggtgatacat taaaaaatga
     1861 aatttggagt tgagaaaatg aaaatcggtg ttattatggg cggggtatcg tctgaaaaac
     1921 aagtttcaat tatgacgggg aatgaaatga ttgctcattt agataaaaat aagtatgaaa
     1981 tagttccaat tacattaaac gaaaaaatgg atttaattga aaaagcgaaa gacattgact
     2041 ttgcattgct cgcattacac ggaaaatacg gagaagatgg gacagttcaa ggaacactgg
     2101 agagtttagg gattccttat agcggaagta atatgttatc tagcagtata tgtatggata
     2161 aaaatatttc gaaaaagatt ttacgttatg aaggtgtaga aacaccagat tggattgaac
     2221 ttacaaaaat ggaggatcta aatcttgatg agttagataa gttaggattt ccattagtgg
     2281 taaaaccaaa ctctggcgga tcgagtgtcg gggtgaaaat cgtttacaat aaaaatgaat
     2341 tgatctcaat gctagaaact gtattcgaat gggattctga agtagtaatt gagaagtata
     2401 taaaaggtga tgaaatcaca tgttctattc tggatggaaa acagttacct atagtttcaa
     2461 ttcgacatgc agccgagttc tttgattaca atgcaaaata tgatgatact agtacagttg
     2521 aggaagttat cgaacttcca gcagaaatta atgaacgtgt aaataaagct tcactgactt
     2581 gttataaagc attaaaatgt agtgtttatg caagagttga tatgatggtg aaggacggaa
     2641 ttccatatgt aatggaaatt aatacattac cagggatgac acaatcaagc ctattgccca
     2701 aaagtgcaga agcagcggga attagttata gcaaactatt agatatgatt attgaaacat
     2761 cattaaaagt tagaaatgaa gaagggtttt aaggagatta tataactaat tcttttgaca
     2821 gtttcacgaa gtatgctatg atgaatacat tataaaaaca aaatatttat ccactagggg
     2881 ggccttttat aggctgagat caaatgtgat tttgagactc ttagtacctg atctggttaa
     2941 tgccagcgta gggaagtggg aaagacgttg ctatttaatg agtaaataaa tactgtcaat
     3001 ttcactttct ttacgcctgt aaagaaagtt tttttatttt acagctttaa taattgtgga
     3061 taaatactta tattttatac attattggag ggatttaaat gactttttca caatcattac
     3121 gtaaagaagt agattcaatt tgggaagcga gttttaacca tccttttgta aaaaaacttg
     3181 gtgaaggaac attagattta gctagttttc gctattatgt gcttcaagat tcatattatt
     3241 tgagtcattt tgctagagta caaactttag gagctgcaaa agctcttgaa ttagagacga
     3301 cagctcgtat ggcacatcat gctcaaaata catatgaggc cgagttatct ttacatgaga
     3361 attttgctaa aaagctaggg ataacaaaag aagaaaaaga taattttatt cctgcgccaa
     3421 ctgcatatgc gtatacttca catatgtatc gtgctgcgta tgaagggcat ttaggagata
     3481 tcattgcagc aattttacct tgctattggt tgtattatga aattggcgaa cgcttaaaag
     3541 agtgccagcc ggaagagcca atttataacg aatggatttc tgcttatgga tctgattggt
     3601 tccgtacgct agttgaagaa caaattacac gtttagatac tatcgctgaa aaagtaacag
     3661 aggtagaccg caaacgtatg aaacagcatt ttattattag cagtcaatat gaatattcat
     3721 tttgggaaat ggcttataca ttagaaaagt ggccagtgga tacagacgta aaagacgtaa
     3781 ttggataaag aatagaaata atgttataga taggtatgga tcatacctat ctatatattt
     3841 tttattgtca aatttgcgta aagtcattag tgctacggat atggcgaaat aaaatatcga
     3901 taatttagca gctcatttac ttgtctacgc attaactgga taaacttctg tcacatttat
     3961 tttacaatca aaggacgtaa atgttggtgg tatgaagtta aataagaaaa gaaacccgag
     4021 ctgttagctg taacggagtt gttttttctt tgcaggaatt tctaaattat catggagtgt
     4081 tgttaatacg atatgcaaat atgcatatcg tatttttttg agttcttcat gtgttaaaat
     4141 tagatataaa gatatggaaa tataaagaag caaaagcagg aatatccaat gaattttgag
     4201 aggtgttata tgtttacaaa taagaaatta attcgatttg gtttatcgtt gtttgtattt
     4261 ttagggataa ttaattttac aatcagctat ttccaaacat atcttgaaac agcagcagat
     4321 attaaatggg taattccaga aatttggaaa actattttgc tagatgttcc tcaaggtata
     4381 cttgttcttt taggcgcaat tgctttatat gatttcacaa aagaggtatc acaaaaagac
     4441 gcatcaatct aagtgtgtct tttttctatt aggaatttca tagagagttt tgctggagaa
     4501 atagaatgaa aagccctaat aagggttttt ttctttgtag gaatttttta tccgtcatag
     4561 aaaccggtta cttggaggtg ttatagcgaa aggcatttac agtacagaaa aatctaagcg
     4621 gcagacggaa atatatagga tatggcaaca taattattga gaaattaatt gaatttagta
     4681 ggtatgaaga tatgacgcta gaattcatat tttttaattt ttatttatcc cgcattaacg
     4741 ggctgttcgt aaaagcctga ttggtgaggg ctaataatca gcgggggatg aactcccccc
     4801 actgattaaa gtttcacttt ataaaaatga gcccatagca aaaataggta tatgaacaag
     4861 gtgcatcatt tagtgataag tcatactttg ttggtgtacg tattgaactt agtttatagc
     4921 gctattttta cttagatgta acgaacagaa atatagggaa gcatcaatga ggttgtattt
     4981 tataaacgga ggttatgaaa atgaatcagt ttcaacaaga actacaagcg ttaagtctta
     5041 atgattaccg atctggaaat attgtctatt gggaccagca aaatcaatat ccatattact
     5101 atattgaaga tgctgctcgt cgctgtggcg gttgtggtcg ttgtggtggt tgtggtggag
     5161 gtcgttgtgg cggattccgt tgtggtggat tccgttgtat tggttgcttc ggttgttttg
     5221 gttgcggagg ttgcggaggt tgtggcggct gtggtggttg ctctaactgt tttgatggtt
     5281 ttagtggtac tactgatact actggtacta ttataacgta tgaatattga ttgcatttat
     5341 cctgctattt gccgggcagt aagaccctaa aaaaattcag cgagagcaaa gtggcggact
     5401 acccataaaa gcccgattgg tgagggctaa ttaaagcttc actttatcaa ttaaaaaaca
     5461 ttagttttat atatgtacgt gccgacaaag aatatagcca ttattaatgg atatattcaa
     5521 tcattcaagt gaaagagtaa caataagata tatagatgta atcaagaggt gattgataaa
     5581 gcaatgacta gatttaaaat ctaatcattg cttttttctt atggttttat catcaaatat
     5641 ctgtctggtt tagggattga tttcttctag ctaatgaatt tattacaact ttgtaaggtt
     5701 aatgtaatgt taatcgatag taagtgaaga tgcttgagtg taatttaaat aagattaaaa
     5761 agtactgttt atctataaaa taattgggta aataatatgt aattaggaag tgtatgaata
     5821 tgattgaact aaaatctatt atccattcgt ataaactaaa gagaaaaata gctaaagatt
     5881 tatacggaaa aagagatgaa ttgacactgt tattaaatga atttaataaa atgaaatgta
     5941 cagtaacatc tgaaaagaag aaaaacaata tattatctcg tttgcaacta atttatcaaa
     6001 atatgaaatt agataagcag tatcctctat cagttgcttt tgatagtaaa ttattggagc
     6061 gattagaaaa agaacctcta catactattg aggatggtgt aacatgttta catctaatgt
     6121 tagatgtgaa ttatgagaaa ataaaacaat atggttcgag tacaagtagg tcatttgttc
     6181 cattatcgca gtcttctatt tgtcttgctg attgtatttg tttaacagga gttgtagtag
     6241 gtttactagg agcaatttca tttgggggat tactgttatc tatatgttca attacataaa
     6301 ggttgatatt tgtaaaaagg gcaccgaatt aaggtgttct tttttggttt ttttgtaaag
     6361 atattgtaaa tgataagtac aatgcattaa gtgtaatgta taattcgaat gtatcaaaat
     6421 ttaaatataa tagaggtgtt atacatgata attactttga atttttaaaa gagtcggttt
     6481 gataataatt tatgtagata aacagttttt acgtttcttt taaatacttg gtttaaatga
     6541 attagattaa gtaactattg ttctagcttt ttgataatca agacaactga agaaaggaag
     6601 gaatcatttt atgaaaaaat taaggctgct aacatttgaa aatatagtag aacccctttt
     6661 aaatgaaaag gtatcattta tatactttcc tattgaatgg ctggacatcg tagagataca
     6721 ttataagaca tttttattaa cgagtaagtt gaaacgtttg aatgaaagat tgtatgatat
     6781 gttttcggat atattgttta ttcagcataa cccgtacgta ttaaatgaaa atacaccatg
     6841 gattgtatcg aaagaaccta ttagacaaga gcagctcgat tatatttttc aaagttggta
     6901 tgagattatt catgattgga aaccaaatag attagtagaa ccaccaaaat atgaatggca
     6961 atccgatttg atttctaatt tgccagtact acatgatatt gaaacgtatt ctaagtgggt
     7021 gcccgcttta atctcacata ttttttgtga gcgtcctata tatttagaaa atacaaatga
     7081 agaagaaatc tatttttccc ctcttagatc acaaaatatt tgtgaggcga tgtcagggcc
     7141 gataaaagat gaaaaaacac aagatttttt ctcctatgta tatcgattcg aatgcataac
     7201 ccgcggtggt gagaacgctc cattattaaa tatttcaata gggattcgga gattttatca
     7261 agaatataag atgataggtc aaacaaacct tgatatgaca acgtttgttt gacttttttt
     7321 gatgaaatgc aaacattttg caaacatagg ttatccaaag atacttttac cgaagttttt
     7381 aactgcttct tcctgcatat ttggtaaaac atgagaatag acacttaatg tcattgaaat
     7441 atctgtatga cctaatcgct cactgatgat tttagggtta actccttgtt ttaatagtag
     7501 agttgcgtgt gtatgtctta aatcatggaa tttaatttct tttataccta ctttgtgtgt
     7561 cgtcctaatg aaactttttc tgaaatgtgc tcttttgatt attcttccaa actcattaca
     7621 atttattaaa tcttgatcta gataagcaga accaaacttt aatttctctt tattgattaa
     7681 aattttatgt ttttttaagg ctgctattgt ttcattaggt acaggaattg tacgttttga
     7741 tgaatttgtt tttgcagttt ttttgatttt attgtcatga ccagatgttt gatttattgt
     7801 aacagtatgt ttttcaaaat caatgtcctg ccatcgtaaa cctaaaactt ctcccagacg
     7861 catacctgta gttattgcaa gtagataccc aatgtgatat cgtgattctt gtgaatgagc
     7921 taaaaacttt tttacttctt cctctgtcca agtctggata ggggtttttt cttttttagg
     7981 tatctcagca aaatctgctg gatttcgaga aataatattt tgttttatgg ctaggtttaa
     8041 agcgctcttt aaaattctat gcataagtag aatagaattg tttgcaatcc ctttatctat
     8101 cgcagtctta tagcattttt gaatgtgcat aacatttaat ttatggagcg cgaccattcc
     8161 tatactaggt aaaacatgta ggttgataaa tgccctatag ccagcaaagg tactcttttc
     8221 tatgctcata cttttaattt ctagccaatg atttagataa tcctttaacg taactttaga
     8281 tggttctata aaagttcctt cattcaactc tgtaatcttc tttgccacat cggcctgtgc
     8341 ttcttttttt gtcttatatc cagaaaacca cttctgtctt ctttttcctg tctctggatc
     8401 aggaccgata tcaataacga tgcaatattt atttcctctt tttcgaatat gtcctttcac
     8461 ttaaaacact ccttcatttt gttttgaatc atgttgtata agtctagttg taattttgct
     8521 gctatgaaaa ttacatgttt ggacatatca gcgatggata tattttacca tataaaaata
     8581 aattcagtta tagtgtagga gattcatttg ttgttaaaca atagtgaaaa gtctactccg
     8641 attattaaat gcaatattgc atatttttta ggaagtctaa aaaacaagga gtatattaat
     8701 atttttaatt ttctcaagta ttttcagaat atgagagaat agatgtttgg gttatttgtg
     8761 atagaatatt cttaataata taatttgacg gaacgaaaaa gacccatagc gtgtgtaact
     8821 gtgctgggaa cacttttaca ccgtttgccc taattgtagt agggaaaaca tttgccatga
     8881 gccttttagt tacgactttg cgtaacatat acggctagta taacacaact tttagatgta
     8941 attcatcatt aaggtgcgtt ttcatgatag ggaagtgtgt cttgttccaa taagggggac
     9001 aaaacatgtg gaaagctcta aatcaaattg aaaaagagtt atgtgcagct ggaataagga
     9061 aaaataaact agcaaattat tggggagtta agccaagtac tgttacaaaa gtttttaaag
     9121 gtaacacaga catgagtttt ggctttcttt ctaagacagt catcctatta aacaaaggca
     9181 tacaggttca agaaaattta ttaacagatt atatacatat aacgaaacca aaatcagaaa
     9241 acttacgtga ggcaatggag gatttagctt taaggggaaa gtttaatcta ttaattaata
     9301 ttataaatag tgagtcacaa tcaaaggtag cagaaaatag agaatttgct aatgtgtatc
     9361 gaattatata tagacgatat ataggtgaga ttgatgctat acagtaccat gaggcattaa
     9421 gcctcgaaag taaatcaata agaacctcag agatggaagt gttaattgaa attttattat
     9481 gtcaagcgca atatcaatcg ggtaatttta ctgctttaaa tgagcgatta aaatctcttg
     9541 aaataaaaat aaataaaata agtaataggt acattcgaga atgttataaa ttacgatata
     9601 aagaagccat tgcggttact tcgttgcagg gtggtgaagt cattgaagca aggcatgtta
     9661 gcatggaact attggatgat cttgaatggg ataacttttt ttcttttccc aaagtaaatg
     9721 catatttaaa gctaggagaa tcgtatattt tttcagcgaa tgaatatgag aaggcaaaat
     9781 attatttgga aaagactctt gaagtgctag gtgatagtaa gattaatgga attgagcgaa
     9841 aaagaaaaat ggtgcaacat acattatcat ttctaaagat tcatcatgat aaagaaatta
     9901 gtagcgtaga tgttgttcat ccaggtgaac tagcatattt aaggattaaa caaggtaaga
     9961 ggattgaagc gagaaaacta ttgattcaat taaaagagaa gaatggaaga ttaacagaca
    10021 tacagactgc atatttaggt ttaacatatg aaggtactaa aaaggaagag ttaatgaaac
    10081 gttctctttt gatgtgtcaa aaatcaggga atatatttta ttcgaattta ccaaaaatac
    10141 acttgggttt aatttgaata attggtataa ttgtcttcgg aaagaggtga aataatgaaa
    10201 aaaataatta caatcattcc tgcgctatta atagctgcta cattattcat taacactgat
    10261 tctataaaag aaaaacctag tacaaatgat ttaaaaccta caattcaaca catgatggtt
    10321 gaccctggtg
//
