LOCUS       NZ_CP018145            23563 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Brevibacillus formosus strain NF2 chromosome, complete genome.
ACCESSION   NZ_CP018145
VERSION     NZ_CP018145.1
KEYWORDS    RefSeq.
SOURCE      Brevibacillus formosus
  ORGANISM  Brevibacillus formosus
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Brevibacillus.
REFERENCE   1  (bases 1 to 6437015)
  AUTHORS   Fu,B. and Li,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2016) Insitute of Enzyme Engineering, Shaanxi
            Academy of Sciences, No 16, East Renmin Road, Xi'an, Shaanxi 710600,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Coverage                   :: 167.68
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 02:46:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,168
            CDSs (total)                      :: 5,986
            Genes (coding)                    :: 5,821
            CDSs (with protein)               :: 5,821
            Genes (RNA)                       :: 182
            rRNAs                             :: 16, 17, 17 (5S, 16S, 23S)
            complete rRNAs                    :: 16, 17, 17 (5S, 16S, 23S)
            tRNAs                             :: 127
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 85 of 165
            Pseudo Genes (incomplete)         :: 95 of 165
            Pseudo Genes (internal stop)      :: 22 of 165
            Pseudo Genes (multiple problems)  :: 35 of 165
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:35:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6315020
            Orig. end                         :: 6338583
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018145.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..23563
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="azole-containing-RiPP"
                     /region_number="18"
                     /rules="((YcaO or TIGR03604) and (((thio_amide and (PF06968
                     or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N
                     or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or
                     PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or
                     thiostrepton or TIGR03602)"
                     /tool="antismash"
     cand_cluster    1..23563
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="((YcaO or TIGR03604) and (((thio_amide
                     and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or
                     (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or
                     TIGR03603 or thiostrepton or TIGR03602)"
                     /kind="single"
                     /product="azole-containing-RiPP"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..23563
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:13563](+)"
                     /cutoff="10000"
                     /detection_rule="((YcaO or TIGR03604) and (((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or
                     (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or
                     TIGR03603 or thiostrepton or TIGR03602)"
                     /neighbourhood="10000"
                     /product="azole-containing-RiPP"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..13563
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="((YcaO or TIGR03604) and (((thio_amide and
                     (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or
                     Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or
                     (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or
                     TIGR03603 or thiostrepton or TIGR03602)"
                     /neighbourhood="10000"
                     /product="azole-containing-RiPP"
                     /protocluster_number="1"
     misc_feature    910..929
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 21.62"
                     /tool="antismash"
     misc_feature    1395..1409
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.41"
                     /tool="antismash"
     gene            1980..2306
                     /locus_tag="BP422_RS30245"
                     /old_locus_tag="BP422_29520"
     CDS             1980..2306
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016740630.1"
                     /locus_tag="BP422_RS30245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29520"
                     /product="DUF1904 family protein"
                     /protein_id="WP_088910738.1"
                     /transl_table=11
                     /translation="MPHLIVRGIQAKQMATISEPLAVELASLCQCGTDNFTIECLHTTG
                     VFGGKIVESFPFIEVAWFERGQEIRDQFAQIVTKHVLSLGIPEVEMAFVAYQKESYYAN
                     GEHF"
     gene            2555..3127
                     /locus_tag="BP422_RS30255"
                     /old_locus_tag="BP422_29530"
     CDS             2555..3127
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012684583.1"
                     /locus_tag="BP422_RS30255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29530"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_088910740.1"
                     /transl_table=11
                     /translation="MEKTEIERLLIEIKAGSLEQYEIIIDHYQQPIFTYCYHMLGHRQD
                     AEDAVQEVLFRAYEHLEQYTYSLSFSAWLYRIAYNHCANVLKRRKLSRVLPFLYNRDQH
                     GRNYVEESIDSNYFGEPLERIWKRLSAEERTLIFLRVLEEREYEEIAELMDKKPAALRK
                     QFERVLKKCKRYLPTTGGVLTDGQKSV"
     gene            3108..4082
                     /locus_tag="BP422_RS30260"
                     /old_locus_tag="BP422_29535"
     CDS             3108..4082
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012684584.1"
                     /locus_tag="BP422_RS30260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29535"
                     /product="DUF4367 domain-containing protein"
                     /protein_id="WP_236841223.1"
                     /transl_table=11
                     /translation="MDKNPFKHMRDMLAAKPVPKVDVKHKVMAVIRAQENKEEKVVKKK
                     IGLLVTVGMLVGASSVWAGMEMIQLKNEKGEVVLELEQMSMQEQQQKMQQLPPDTKDQI
                     KNRNAEIFKQLDTGEQIVNGLKPGEAVAIYWPLTKLQLESNPNSNPFVDVRSKPFTYTT
                     WKDMEQKVGHLFKVSAEMGTDFKFVAGEAIYLPSDKYDEKAMKEEQERTKKEYVVQPIP
                     LSDKLRNAEIEYKGQKGSAFIEVSILDDKNKSGGTFAENLEKIMIGDNEAVYSTFAPKN
                     GDTVHSLEWVKNGTKVNYSLRAKMSVSTKEELLKMAEQINDTK"
     gene            complement(4143..5066)
                     /locus_tag="BP422_RS30265"
                     /old_locus_tag="BP422_29540"
     CDS             complement(4143..5066)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012684585.1"
                     /locus_tag="BP422_RS30265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29540"
                     /product="EamA family transporter"
                     /protein_id="WP_088910742.1"
                     /transl_table=11
                     /translation="MVLLNYVVMCLIFGTTFLAIKVGIDAGAPPFFSGGLRFFLAGVIL
                     FSFMLWKKQARLSLLFHKEMFLTGIGLTFGTFAPLYWAEQHVSSGIAAILSATGPLMVM
                     LLQTGITRQKPPGIALAGCLVAFVGVFLLILPGVTISFSLLWLTGCMAVIFGEIFYSAG
                     AVYSRSVIQRFQDTSPIALNAAQMMYGGAMLLVLSLFTEQVHIESMLTANAILSLLYLI
                     VVGSMMGHTIFYWLVAKTNPVFPSTWLYISPLIALGLGVILYNEPLYLLSLAGGITIIV
                     GIILINIDGLKQLMGKKANKLSDISH"
     gene            5178..6605
                     /locus_tag="BP422_RS30270"
                     /old_locus_tag="BP422_29545"
     CDS             5178..6605
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (144-464). E-value:
                     8.2e-36. Score: 115.8. Matches aSDomain:
                     nrpspksdomains_BP422_RS30270_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721934.1"
                     /locus_tag="BP422_RS30270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29545"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_088910743.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 7.3e-34, bitscore:
                     115.9, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKREVLSNSHSDKLFEQVYDYLLDRIRREEWRAHEKLPSVRTLAL
                     DLNVHRLTVFKAFQLLKQNRHVYVKDKSGYYVQPSSLLPVESMNDPIVSAYVHKSHLSE
                     IHQVQATYQFSKALIEPNLLPNHYFSEYVKKVFDLYPKVLGTYSTTQGDQELREALCSY
                     YVSRYHFHLSPDELMVTSGSQEAIDLVARVLVKPRDVVLLERPTYSPAIDVFRRQGVKM
                     IPIEIKPHGYDLEQVELLMQKEKPRLFYLNPTFHNPTGYTIPAEQRKRLVELAAKYQCL
                     IVEDDPCRDIYFGDEPPLPFFSYDTAGYVIYLRSFSKYIAPGLSIATVACRPSIMKYLI
                     QAKSLSDSGAPLLTQKIFLHYFFSERMQQHLAKLRIALAIRRDITEEELSVTDWEWSSP
                     PGGFNLWVKLPDSVPMESLLGKCIEQSITFVPGAICDPLRELSSWIRISYSYLNEQQLR
                     DGLQRLVRTVRQLDIKK"
     aSDomain        5610..6569
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BP422_RS30270_Aminotran_1_2.1"
                     /evalue="8.20E-36"
                     /label="BP422_RS30270_Aminotran_1_2.1"
                     /locus_tag="BP422_RS30270"
                     /protein_end="464"
                     /protein_start="144"
                     /score="115.8"
                     /tool="antismash"
                     /translation="GTYSTTQGDQELREALCSYYVSRYHFHLSPDELMVTSGSQEAIDL
                     VARVLVKPRDVVLLERPTYSPAIDVFRRQGVKMIPIEIKPHGYDLEQVELLMQKEKPRL
                     FYLNPTFHNPTGYTIPAEQRKRLVELAAKYQCLIVEDDPCRDIYFGDEPPLPFFSYDTA
                     GYVIYLRSFSKYIAPGLSIATVACRPSIMKYLIQAKSLSDSGAPLLTQKIFLHYFFSER
                     MQQHLAKLRIALAIRRDITEEELSVTDWEWSSPPGGFNLWVKLPDSVPMESLLGKCIEQ
                     SITFVPGAICDPLRELSSWIRISYSYLNEQQLRDGLQRL"
     gene            complement(6685..7392)
                     /locus_tag="BP422_RS30275"
                     /old_locus_tag="BP422_29550"
     CDS             complement(6685..7392)
                     /EC_number="3.4.24.-"
                     /GO_component="GO:0031012 - extracellular matrix [Evidence
                     IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /locus_tag="BP422_RS30275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29550"
                     /product="matrixin family metalloprotease"
                     /protein_id="WP_088910744.1"
                     /transl_table=11
                     /translation="MSNSITRRKTAHFSLNVIVQKGAFEDLEIAKSTVKQNVVYAQHYF
                     DRNAEVNGFPWMNLVFHEENDVFENDRLQHSFNSSHDLRDFFIETARAGGWRKTKQRLP
                     CINIYYLIAIPPDGISAGVHIPGAATYCNLRAIEKLSLLLQKWKRASPSEHLALYGIIK
                     HRPSVFLNANAIAPSTLAHEIGHILGLGHSSEPSNLMYPIANQRIRCELTYEQSICLRD
                     GIWKYSHGYFPFL"
     gene            7686..7913
                     /locus_tag="BP422_RS30280"
                     /old_locus_tag="BP422_29555"
     CDS             7686..7913
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BP422_RS30280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BP422_29555"
                     /product="hypothetical protein"
                     /protein_id="WP_088910745.1"
                     /transl_table=11
                     /translation="MILGIYTNIDAETDAVVETDVMTDAVGETDVMTDAVGETDAVVET
                     DAVVETDAVVETDVMTDADADVGTDVETGV"
     aSDomain        8085..8381
                     /aSDomain="Heterocycloanthracin_C_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing C protein in a
                     heterocycloanthracin LAP cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_BP422_RS30285_Heterocycloanthracin_C_
                     RRE.1"
                     /evalue="3.20E-42"
                     /identifier="RREFam011.1"
                     /label="BP422_RS30285"
                     /locus_tag="BP422_RS30285"
                     /protein_end="99"
                     /protein_start="0"
                     /score="133.4"
                     /tool="antismash"
                     /translation="MTSVNPSMRLKVKGDTYYVPNPDGRVYFQNNLGSFQMEGKGIHKW
                     IEKLMPLFNGEHTLEELTNGLSDQRRNHIYHIADMLYQNGFVHDWSSQLPHQLS"
     gene            8085..10001
                     /locus_tag="BP422_RS30285"
                     /old_locus_tag="BP422_29560"
     CDS             8085..10001
                     /GO_component="GO:0005575 - cellular_component [Evidence
                     IEA]"
                     /GO_function="GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /GO_process="GO:0030152 - bacteriocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03693"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Heterocycloanthracin_C_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thiopeptide_F_RRE"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003340798.1"
                     /locus_tag="BP422_RS30285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29560"
                     /product="putative thiazole-containing bacteriocin
                     maturation protein"
                     /protein_id="WP_088910746.1"
                     /sec_met_domain="TIGR03693 (E-value: 1.2e-206, bitscore:
                     686.2, seeds: 2, tool: rule-based-clusters)"
                     /sec_met_domain="Heterocycloanthracin_C_RRE (E-value:
                     6.8e-40, bitscore: 133.4, seeds: 5, tool:
                     rule-based-clusters)"
                     /sec_met_domain="Thiopeptide_F_RRE (E-value: 1e-16,
                     bitscore: 59.1, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSVNPSMRLKVKGDTYYVPNPDGRVYFQNNLGSFQMEGKGIHKW
                     IEKLMPLFNGEHTLEELTNGLSDQRRNHIYHIADMLYQNGFVHDWSSQLPHQLSKEIME
                     KYAPQIEFLNSFTDSAAYRFQLYRQTKVLAVGSGPLLSALVAALLESGLPAIYIATTHS
                     IETNRERLWELAAHARKEDSEVKLEEIYLQKNDRDGWQKAVQTFQVVLYVQQEGDLEEL
                     RVLYSICKEQNKIFIPAICQDQVGIAGPVLRPDADGCFESARRRIHRSLFNNGSCFSTY
                     SPISAAMLANIVVFELLKTITGTSGQENKIFLMNLETMEGEWHPFTPHPLVTGGARVKW
                     VQDTNLLFEQRLHQNGSYDLQAFFGSLTSDQTGIFHTWDEGDLEQLPLAQCRVVVVDPL
                     SEGPVELLADIVCSALTHDGARREAGLAGIEAYASRMAKPLFGTEEFVGIGAGETVPEA
                     VTRGLQKSLTAELRKQLENHQTAVYPVELNSIEDKHSLFYLQALTIMNGPPTVCLGEEI
                     SGFPVVWIGTNDRWYYSVCLHLTMGLRTVLQQALWVNHNSSLSQTTTYGINPSSIVVKE
                     KLPHSLVIPTVEEAAHSEMLQAALSSLRRNNKELFVCELELEQVFKEELAGVFGVLLRK
                     DES"
     aSDomain        8106..8348
                     /aSDomain="Thiopeptide_F_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing F protein in a thiopeptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_BP422_RS30285_Thiopeptide_F_RRE.1"
                     /evalue="4.80E-19"
                     /identifier="RREFam004.1"
                     /label="BP422_RS30285"
                     /locus_tag="BP422_RS30285"
                     /protein_end="88"
                     /protein_start="7"
                     /score="59.1"
                     /tool="antismash"
                     /translation="MRLKVKGDTYYVPNPDGRVYFQNNLGSFQMEGKGIHKWIEKLMPL
                     FNGEHTLEELTNGLSDQRRNHIYHIADMLYQNGFVH"
     gene            10001..11944
                     /locus_tag="BP422_RS30290"
                     /old_locus_tag="BP422_29565"
     CDS             10001..11944
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013877478.1"
                     /locus_tag="BP422_RS30290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29565"
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="WP_088910747.1"
                     /sec_met_domain="YcaO (E-value: 1.1e-78, bitscore: 263.7,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 1.1e-122, bitscore:
                     408.0, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTVLMIVGEGLLADFVYRNLSCQYSVVQQPDCKADIPKEVELAL
                     VLHDFWHPSIHLQAEEVFKKAGIPWLRGFVSFGEGVVGPLVGPRMQGCPQCADFRRLMT
                     RKNRRDLREIQLQLKNQKKRQRDAWASNTGMYQLANMITAEAQRVLQGSRPYTVQRTRI
                     HLVNLQTLATSEHFFLPNPICEVCGELPEDTPEAARITLQPRPKANPNSYRICSLDVFK
                     EVLIQEYLDFRTGMINQKTKDHLTPFAVMSVNLPMITSNEGAAGRTLSYEQSELTAILE
                     ALERYAGLHPRGKRTVICDSYEKVKDHALNPLKIGLHLEEQYASPGYPFQRFDPKRPMN
                     WVWGYSFFKEKPILVPESIAYYALEDDFVYENSNGCALGRCLEEAIFYGILEVVERDAF
                     LLTWYAQLPLPRLDPESAKDKELLLMIERIKAVAGYDLYLYNATMENGIPSVWAITKNR
                     KQKGVNLVCAAGSHPDPLRAVKTAVHELADMLLTLDEKYETHREEFLQMLHDPFRVQSM
                     ADHGMLYSLPEAEERLHFLLHDDRPMRTFDEEFKLQAGHHDLTDDLKEILQIFKQLNLE
                     VIVVNQTTPEMIRNELYSVKVLIPGMLPMTFGYHLTRLTGLDRVLNVPMQLGYTKHPLT
                     YEQLNPHPHPFP"
     gene            11968..13563
                     /locus_tag="BP422_RS30295"
                     /old_locus_tag="BP422_29570"
     CDS             11968..13563
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     azole-containing-RiPP: PF00881"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676357.1"
                     /locus_tag="BP422_RS30295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29570"
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="WP_088910748.1"
                     /sec_met_domain="PF00881 (E-value: 1.6e-06, bitscore: 26.7,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRDVFLHNLLYDIEKIIPADWTVEWEDSPLVYKIYRELPVVPLS
                     LEVRLSLEHGRNKQSSKPTIRDIGHFLWVTYGLTRVSDPGNDWNSFEQRSEELFRRFVP
                     SGGALYPSELYVYLNMDDLPVGVYHYDAAHHRLVLLRKGNCDSYLTMALGYNCDMSECF
                     AAVFVSTMFWKNFFKYHNLSYRLQGLDAGVLIGQLLEAAKRFGFTTGVYYQFLDRAVNH
                     LLGLNQWEESVYAVIPLSIKSDINWFAHKREGMEIVWDKELYRKLPAVQHHSYIRSRRI
                     RKYPMLIKLNEASKFESTDCFHRVRGEKKPVFGGPSLALPNVKPLNWDLMEASRKRFSP
                     GEHFVLREVSQQQLATLLREATNAFFYRNDLDRIDIPEIQEQPRSRVSLYCCLYSVEDI
                     PAGAYYYDYPSHKLRLIRLGDHRPILKQAINRSDFNILDVPICLHVVGDYDYYRHKLGY
                     RGYRIQQMEAGILVQKLLLVASSIGLAGHPLLEYDAKTCDKIYKITNVNQSCLIQVPVG
                     PYRLPIRLTGRLYI"
     gene            complement(14055..14255)
                     /locus_tag="BP422_RS30300"
                     /old_locus_tag="BP422_29575"
     CDS             complement(14055..14255)
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1255: cold-shock
                     DNA-binding domain protein"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721941.1"
                     /locus_tag="BP422_RS30300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29575"
                     /product="cold-shock protein"
                     /protein_id="WP_088910749.1"
                     /transl_table=11
                     /translation="MEQGTVKWFNAEKGFGFIEREGKEDVFVHFSAIQSEGFKSLDEGQ
                     RVTFDVEQGQRGPQATNVQKV"
     gene            complement(14469..15155)
                     /gene="rluF"
                     /locus_tag="BP422_RS30305"
                     /old_locus_tag="BP422_29580"
     CDS             complement(14469..15155)
                     /EC_number="5.4.99.21"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rluF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1154: Pseudouridine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721945.1"
                     /locus_tag="BP422_RS30305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29580"
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="WP_088910750.1"
                     /transl_table=11
                     /translation="MRINKFISETGICSRREADKLIEAKRVTINGQLAELGSTVALGDD
                     VRIDGQPLGAKKKDVYIALNKPVGITCTTELHVKGNIIDFVNHPERIFPIGRLDKDSQG
                     LILLTNDGDIVNKILRAENNHDKEYIVTVDKPITANFLHGMANGVRILGTTTKPCKVTK
                     VSDRVFNIVLTQGLNRQIRRMCQAFGYQVRKLERIRIMNITLGNLKLGQWRNLTAKELT
                     DLNKSL"
     gene            complement(15174..15821)
                     /locus_tag="BP422_RS30310"
                     /old_locus_tag="BP422_29585"
     CDS             complement(15174..15821)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016740636.1"
                     /locus_tag="BP422_RS30310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29585"
                     /product="MOSC domain-containing protein"
                     /protein_id="WP_088910751.1"
                     /transl_table=11
                     /translation="MNRKIVTISKGLPKSNMYNGKEYLSGIWKEEANELIVRSEKIDDD
                     DIANPEYHGGPDRVVCAYPFEHYAHWEKLFGHPLTNAAFGENLTLAGMTEEQVCIGDVY
                     QFGDTILQVTQGRFPCATINKRNNNNQLLKAVVELGYTGYFFRVLQEGTIKPDSKITLI
                     TAHPKQVTVASIHHLYFHDKTPSQEMIQRMLEVEELALPWRNKLLEKLEKQQ"
     misc_feature    15889..15906
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 23.05"
                     /tool="antismash"
     gene            15967..17415
                     /locus_tag="BP422_RS30315"
                     /old_locus_tag="BP422_29590"
     CDS             15967..17415
                     /EC_number="6.3.4.21"
                     /GO_function="GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017249971.1"
                     /locus_tag="BP422_RS30315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29590"
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="WP_088910752.1"
                     /transl_table=11
                     /translation="MQSFGVALHTDKYQINMMYAHWVNGTHNQKVVFEAFFRKLPFGNG
                     YAVSAGLERIVSYIKNLRFEESDLQYLSEQEESYDPKFLEELRQLRFKGSLYSVKEGTV
                     VFPNEPLIRVEGRVMEAQLVETALLNYMNYQTLIATKASRIKNVAGNDVLMEFGTRRAQ
                     EADAALWGTRAAYLAGFDATSNMLAGKMFGIPTKGTHAHSWVQSFESEEEAFERFAAAL
                     PGQVTLLVDTYDTLKSGVPNAIKVGKRLEKQGKKLTAVRLDSGDLTYLSCKTREMLDKA
                     GMTDVQIVASNDLDEHTIMNLKVQGARVNSWGVGTQLITAADQPTLGGVYKLVAREGAD
                     GKYQPTIKISGNPEKVTTPGIKDMYRLIDPETGKAIADYMCFPHETTVDDRAPLHLFHP
                     SHTYLSKHLENYQAESLLVPVFVDGELVYELPTLQEIRTYHREQLKLFWPEYLRMLNPE
                     HYRLHISEEMWETKTKLMKAYLKK"
     gene            17612..18871
                     /locus_tag="BP422_RS30320"
                     /old_locus_tag="BP422_29595"
     CDS             17612..18871
                     /EC_number="1.4.1.-"
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721951.1"
                     /locus_tag="BP422_RS30320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29595"
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="WP_088910753.1"
                     /transl_table=11
                     /translation="MLNTVEKENLNPYEIVQKQIDAAAALLGLRSDAVEILKRPKRVLA
                     VSFPVKMDDGSVRVFEGYRSQHNDAVGPTKGGIRFHPDVTMDEVKALSMWMSFKCGVVG
                     LPYGGGKGGVICDPHEFSKNELERVSRGFMEAIADIVGPETDIPAPDVYTTPQIMGWMM
                     DTYSRLKGTYSPGVITGKPLSVGGSKGRNEATARGCVFTILEALKDSGRKPEQTTVAIQ
                     GFGNAGRIAARLLTELGFKIVAVSDSRGGIYDAAGLDIEKVGQLKDDATILEYVGGTVI
                     SNEQLLELEVDILIPAALENVITAANAHSIQAKWIAEAANGPTTPDADAILREKGITVI
                     PDILANAGGVTVSYFEWVQNLMNYYWSEQEVNEKLQTTMINAYRAVKELSDQYKVDLRT
                     GAYMISLLRITEAMEARGWI"
     misc_feature    complement(18884..18900)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 25.46"
                     /tool="antismash"
     gene            19013..19933
                     /gene="metA"
                     /locus_tag="BP422_RS30325"
                     /old_locus_tag="BP422_29600"
     CDS             19013..19933
                     /EC_number="2.3.1.46"
                     /GO_function="GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="metA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016740639.1"
                     /locus_tag="BP422_RS30325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29600"
                     /product="homoserine O-succinyltransferase"
                     /protein_id="WP_088910754.1"
                     /transl_table=11
                     /translation="MPIKLPDELPATEILAQENIFVMKDSRAFTQDIRPLRIIILNLMP
                     VKETTETQLLRLLGNTPLQVEIVLLHMSSHTSKNTSEEHLSMFYKTFEEIRDQRFDGMI
                     ITGAPVEQLEFTDVTYWQELTEILDWKMENVTSTLHICWGAQAGLYHHFGVPKHPLPEK
                     MFGIFPHTLNKQNVKLLRGFDNYFHIPHSRHTENRREDIEQVPELEILSESDEAGVYIV
                     ATRDGRQIFVTGHSEYDPTTLQDEYQRDVNKGLDIAVPRNYYPKDDPSREPIVTWRAHA
                     NLMFSNWLNYYVYQETPYDLNNDKR"
     gene            20068..21093
                     /locus_tag="BP422_RS30330"
                     /old_locus_tag="BP422_29605"
     CDS             20068..21093
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1108: ROK family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005832122.1"
                     /locus_tag="BP422_RS30330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29605"
                     /product="ROK family transcriptional regulator"
                     /protein_id="WP_088910755.1"
                     /transl_table=11
                     /translation="MKKIMKQDQDVTKQHNKQMILDIIKKQRPISRAEIAKITRMSATS
                     IGRFVSELCEEGLIRETELTSSGVGRKAIMLDIEPGAIYTIGVDMAKKRIGIGMPGVIN
                     YERGIVQLSTTLDWKDVAFANLIEKRLKIKTVIDNDLKVKILSEYLYGSAKGSNKTALI
                     GIGTGIGSALIINGEVFRGGSNSAGELGHTTLDPNGNLCECGKRGCLQTYIDENSLIME
                     ARRVKDVNDDRQVFAAARNEERWAKEIVSRTALYLGITINNIVCMYNPDTVIVSGDLVE
                     NYREIIDLVEEHSDQVIWEPFKGKFRVIPSELKGEAIVMGAGALAMQHFAPLAGRWAEE
                     V"
     gene            21181..22293
                     /locus_tag="BP422_RS30335"
                     /old_locus_tag="BP422_29610"
     CDS             21181..22293
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017249975.1"
                     /locus_tag="BP422_RS30335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29610"
                     /product="Ig-like domain-containing protein"
                     /protein_id="WP_088910756.1"
                     /transl_table=11
                     /translation="MRNWQKGMLATAVTVGLIFSSVGQVPALANSATTSTQKKISKLVV
                     DTKTVKLKKTGTQQLNVRVQYTDKTTEDVTQQAEWTSSDSDIATVDKGLVTAVSSGKTR
                     VKASFGGKSVNVPVEIDVISKLSADQKKLALSVGGTKQVNATATFSDKSTADVTAQAEW
                     ESDDTEIVTVTNGLVTAVGSGKAKLKVKYGGKSVTIPVEVDVVSKLDSDKKKLYLRPDT
                     TQSVTLLATLSTKEKVDVTSKAKWTSDDAKVATVENGVVTTVGSGKTRIKATYGGKTIS
                     IPVESAVISKLEFDTKKADLKVGESKDLKVSVVYTDKTKVDVTIDADWISTNSSVVTVA
                     DGKITAVKEGRATIVATYNGKVVKIQVTVK"
     gene            22405..23301
                     /locus_tag="BP422_RS30340"
                     /old_locus_tag="BP422_29615"
     CDS             22405..23301
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721976.1"
                     /locus_tag="BP422_RS30340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_29615"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_088910757.1"
                     /transl_table=11
                     /translation="MDIRQLHYFVEVVKHKNFTKAAQSLHVSQPSISKMIKALEEELEV
                     VLLDRTERKMDLTEAGEMVYNYATKVLQLMESMSSSIAELRNVERGRVKLGMMPTVGSF
                     LLPNVIALFKKQYPGIDIEMKEYSAKLLEIQMEQGSIDVALTVLPTDQEKFVAVPLLAE
                     DLVAIVHREHWLAGVDEVSLEQLKDEAFILFTEEYAMHDVVRQACKLSGFEPKVAYMSS
                     LWDFVGEMVATQLGISLIPRSIVRRLNHGQLHTVNISYPVIDWQYALIYRKEGYLSHAT
                     KAFISFVEKMYSHNQME"
ORIGIN
        1 atttccttgg tatgagacgg cacagcatga ggattccgtg ttatacaaag ggaagtacta
       61 cacgagacag gatcacttgg cagcagggga gagcacaggt ggggaagttc gtgttttaca
      121 ggtgggacgc gactattatt tcttgaatca ggcgatttcg tatgatgggc agcgctccat
      181 tgaggatggg aagcttgtca ttgtcaaagg tgcggatcga gcggagtatc aggtgactct
      241 cttaagcaat caggagatat ttgaattttt ccggccagag acaagcggct tgctcacact
      301 ggctgctctc tatttcggat tattggtagt tgcctcactt ttccattatg ggcaaaaatt
      361 tttatggcaa aaatcagcga atcaggtcat cgagagactg cgcatggacg tgttcgcgca
      421 tattcaacga ttgcccattc aatatttcga tcatctccct gccgggaagg tggttgctcg
      481 tgtcacaaac gatacggagg caatccggga gctgtttacg aatgtactcg gcaactttat
      541 tacgagcggg atttatttaa tcggtatttt cgcagcgctg ttcttacttg atgtacgact
      601 cgcgctcgtt tgtctggtca ttctgccaat catggtcgtg tggatttact tgtaccgcaa
      661 gtacgcttct gcattcaacc atgtcattcg ggcacgtatc agcgacatca acgggatgat
      721 caacgagtcg atccaaggca tgccgatcat tcaggcgttt cgcagagaaa aggaaacgat
      781 gcgggaattc ggcgaactga atgaagagct gttcacctat caaaataaaa tgctacggct
      841 caatgcgacc gcttcgttta acctggttca gttcatccgg aatatcgcgt ttgtgttgtt
      901 catctggtat ttcggaggag cgtcccttgg gattggtaca ctgctgtcgc ttggtgtcct
      961 ctacgcattc gtcgattatc tcaaccgttt gttccagccg atgacacaga tcgtcaacca
     1021 attggcgaac ctggagcaat cactcgtcgc ggcgtcgcgt gtgtttgaac tactggatga
     1081 agagggcgtt gacgttgatc ctgcaaaagt tccctgctat caggggaatg tccgctttga
     1141 cgatgtttct tttgcctaca aagacgacca atacgtgctg aaaaacattt catttgaagc
     1201 atggcctgga gagacagtag cactggttgg tcatacgggt tcgggaaaaa gctcgattat
     1261 caatctgtta ctccgtttct atgacgtaaa cagtggagcg attatgattg atgggcgcaa
     1321 tgtgcagtcg ttttccaaac agcagcttcg tcagcatatg gctattgttt tacaggaccc
     1381 gtttttgttt acaggaacga ttcgttccaa tgtgagtctg gatgatccgt cgatttcgcc
     1441 ggaaaaagtg gagaaggcct tgtgtgatgt aggggcagac caactgttcc gcaatcttcc
     1501 gcaaggattc gatgaacccg ttatcgagaa gggcagcacg ctttcactcg gacagcgtca
     1561 gttgatttcg tttgcgcgtg ctctcgcgtt tgacccggct atcctcattc tggatgaagc
     1621 gactgccagc atcgacacgg aaacggaggg cattattcaa aatgcacttg aggtgttgaa
     1681 aaaagggaga acgaccttca tcattgctca tcgcctgtct acgatcaaaa atgcagatca
     1741 aattctcgta ctggatcgag gcagaattgt agagcgagga aagcatgaag aattgatgga
     1801 gaagcaaggc agatactacc agatgtatca attgcagcaa gggaacaaag aagtgcgggt
     1861 gcaagaggca gtaggtaagc acgggagcgc cgcagctctg acctagtttt ttctactctt
     1921 tttgcctctt gaacggtaag atagagcata gaagtatgga cttttcagga gggatcatca
     1981 tgccacatct tatcgttcga ggcattcaag ctaaacaaat ggctacgatt agcgaaccgc
     2041 ttgcagtgga gctagcttca ctctgccaat gcggtacaga caatttcacg atcgaatgct
     2101 tgcatacaac aggcgttttt ggcgggaaga tcgtggagtc gtttcctttt atcgaagtag
     2161 catggtttga gcgggggcaa gaaattcgtg accagtttgc ccaaattgta acgaagcatg
     2221 tcttatctct tggcatccca gaagtagaga tggcatttgt ggcttatcaa aaagaaagct
     2281 attatgccaa cggagagcat ttctaaagag cagccgacag gtgctgtcga tttcccggga
     2341 aagaatcaag acattcgaca agagtacgga caaataaatg aatacaagca tggaataagg
     2401 gtgttccttc cagaagtacg agatttttcg tgcgaaatgg gtggagccct tttttatttg
     2461 gggcgaattt taattcatcg aaccaaaaat ttctaaaata ggtgtcacaa acaaaagata
     2521 ccagttgtta ttcatagtga gaggagggca aacagtggaa aaaacagaga tagagcgcct
     2581 gctgatcgaa ataaaagccg gttctcttga gcaatacgaa atcattatcg atcactatca
     2641 acaaccgatc tttacatact gctatcacat gctcggtcat cgccaagatg cggaagacgc
     2701 tgtccaggaa gtcctgtttc gtgcctacga gcatcttgaa cagtatacgt actcgctgtc
     2761 attttccgca tggctgtacc ggatcgctta caaccattgt gcgaatgtgc taaagcgcag
     2821 gaaattaagc cgcgtgttgc cctttttgta caatcgggat cagcatggac gcaattatgt
     2881 cgaggagagc atagacagca attatttcgg ggagccgctc gaacgcatct ggaaacgact
     2941 gtccgccgaa gaacgaacac tcattttcct gcgtgtgcta gaggaaagag agtacgagga
     3001 aatcgcggag ctgatggaca aaaaacctgc tgccctgcgc aagcaattcg aacgcgtttt
     3061 gaaaaagtgc aaacgttatt tgccaacgac gggaggggtg ctgactgatg gacaaaaatc
     3121 cgtttaagca catgagagac atgctggcag cgaagccagt cccgaaggtc gatgtcaaac
     3181 ataaagtcat ggcagtgatt cgtgcccagg aaaacaagga ggaaaaagtc gtgaaaaaga
     3241 aaattggctt gctagtcact gtaggtatgc tggtcggagc gtcgtccgta tgggcgggga
     3301 tggaaatgat ccagttgaaa aatgaaaagg gcgaagtagt gctagagctt gaacaaatga
     3361 gtatgcaaga acagcaacaa aagatgcagc agctaccccc tgacacaaag gatcagatca
     3421 agaatcgaaa tgcagagatt tttaaacagt tggatacagg tgaacaaatc gtcaatggat
     3481 taaaaccagg tgaagcggtt gccatttatt ggccgctgac aaaacttcag ttggaatcaa
     3541 atccaaacag taatccgttc gtagatgtac gatccaagcc ttttacctat acaacgtgga
     3601 aggacatgga acaaaaggtt ggccatctgt tcaaggtctc tgcagagatg gggacggact
     3661 tcaagtttgt agcaggtgaa gcgatctatc ttccaagtga caagtatgat gagaaagcga
     3721 tgaaagaaga acaagaaaga acgaaaaaag aatacgtagt acaaccaatt ccgttatcgg
     3781 acaagcttcg taatgcagag atagagtaca agggccaaaa agggagtgct ttcatagaag
     3841 tttcgatcct ggatgacaag aataaaagtg gaggtacatt tgcggagaat ctggagaaga
     3901 tcatgattgg cgacaatgaa gccgtgtact ccactttcgc acctaaaaat ggcgacaccg
     3961 ttcactcttt ggaatgggtg aaaaatggga caaaggtaaa ctatagcctg agagccaaga
     4021 tgagtgtaag cacgaaagaa gagttgctga aaatggcgga acaaatcaac gatacgaaat
     4081 aagcatcttc caaatagagg agtaccacaa agcgccagtt ttgtggtact ccttttctat
     4141 ctctaatggc ttatgtcact taacttgtta gcctttttgc ccatcaactg cttcaagcca
     4201 tcgatattaa tcaggataat gccgacaata atcgtgattc cccctgctaa cgacaggaga
     4261 taaagcggct cattgtacag gatgacgccc aagcctaagg caatcaacgg agagatatag
     4321 agccacgtgg agggaaacac agggttggtt ttggcaacga gccaatagaa aatggtgtgt
     4381 cccatcatag aaccaacgac gattaagtat aggagcgaga ggatcgcatt agccgtgagc
     4441 atcgattcga tatgcacttg ctccgtaaat aacgataaga caagcagcat ggctcctcca
     4501 tacatcatct gtgccgcatt caaagcgatg ggtgaggtat cttgaaaccg ttggatgacg
     4561 ctccgggagt atactgcacc agcagaataa aagatttccc cgaatatgac tgccatgcac
     4621 cctgtaagcc aaaggagact gaatgagatg gtcacaccgg gtaaaatcag caaaaagaca
     4681 ccgacaaagg caaccaagca acccgccaaa gcaataccgg ggggcttttg ccgagtaata
     4741 ccggtctgaa gcagcatcac catgagaggg cctgttgcag ataatatcgc tgcaattcct
     4801 gaacttacat gctgctctgc ccaatacaag ggggcaaatg tcccaaacgt caaaccgata
     4861 ccggtaagga acatttcttt atgaaacagc agcgagagac gcgcctgttt tttccagagc
     4921 atgaatgaaa acaaaatgac acctgccaga aagaaccgaa gtcctcctga gaaaaacggc
     4981 ggcgctcccg catcgatccc gaccttgatt gccaaaaatg tcgttccgaa tatcaagcac
     5041 ataaccacat aattaagcag taccattatg attcctccct tttcctcatc ataaagggaa
     5101 ggaaacagaa cagatgaaat ggaatagaac agatacgaag atcactctat tatcataggg
     5161 gaaagggggg agcttgcatg aaaagggagg ttctttcaaa cagtcactct gataagcttt
     5221 tcgaacaagt gtatgactat cttctggacc gaatcagacg cgaagagtgg agggcacatg
     5281 agaagcttcc ttctgttcga acgttggcgt tggacttgaa tgtgcatcgc ctgacggttt
     5341 ttaaagcatt tcaattatta aagcaaaacc gccatgtgta tgtaaaggat aagtcgggat
     5401 attatgtaca accgagcagc ctgctgccag tggagtcgat gaatgatcca atcgtttctg
     5461 cctacgtgca caagagtcat ctttctgaaa ttcatcaggt gcaggcgacc tatcaatttt
     5521 ccaaagcatt gatcgagccg aatttattgc caaatcatta cttttccgag tacgtgaaga
     5581 aagtgtttga tctgtatcca aaggtactgg gtacctactc aaccacacag ggagatcagg
     5641 agctgcggga agcactgtgc agctattatg tcagtcgtta tcattttcat ctgtcacccg
     5701 atgagctgat ggtcacatcc ggctcacagg aagcgattga tctggttgcc agagtccttg
     5761 taaagccgag agatgtcgta ttgttggaga gacccactta cagtcctgcc atcgatgtat
     5821 tcagaagaca gggcgtcaaa atgattccca tcgagatcaa gccgcatgga tatgatttgg
     5881 agcaggttga actgttgatg caaaaagaaa agccccgcct gttttatctc aatcctacct
     5941 ttcacaatcc aactggctac acgattcctg ctgaacaacg aaaacgattg gtggagctgg
     6001 ctgcgaagta tcagtgtctc atcgtggagg atgatccgtg ccgcgatatt tactttggcg
     6061 atgagcctcc actgccgttt ttttcttacg acacagcggg ttatgtcatc tatctgcgca
     6121 gctttagcaa gtacatcgca ccgggactga gtattgcaac tgtggcatgc cggccatcca
     6181 ttatgaagta tttgattcaa gcgaaatcat tgtccgacag cggagcgccc ttgttgactc
     6241 aaaagatttt tttgcattat ttcttctccg aacggatgca gcagcattta gcgaagctgc
     6301 ggattgcact ggccattcgc cgggatatca cggaggaaga gctgtccgtt accgactggg
     6361 agtggtccag cccgccagga ggctttaatt tatgggtgaa gctgcctgat tctgtcccga
     6421 tggaaagtct tttggggaaa tgcatagagc aatccataac gtttgtgccc ggtgcgatct
     6481 gtgacccgct gcgggagtta agctcttgga tacgtatcag ttactcttat ttgaatgaac
     6541 aacagctgcg ggatggtttg caaaggctcg tccgtacggt tcgccagttg gatataaaga
     6601 aatgaagaaa ggctaccgac atgatggtcg acagcctgta gcccactcgt ataacaggtg
     6661 ggcttttttc ttgttttgcg atcgtcatag gaacggaaaa tacccgtgac tgtacttcca
     6721 tattccatct ctcaaacaaa tgctctgttc ataggttagt tcacatcgaa ttcgttgatt
     6781 agctatgggg tacataaggt tactcggttc tgaactgtgg cctaatccca agatgtgacc
     6841 aatctcatgg gccaatgtag atggtgcaat agcgtttgca ttaagaaaaa cggaagggcg
     6901 atgcttaata ataccgtaca aggcaaggtg ttctgaaggg ctggccctct tccatttctg
     6961 cagcaacaaa gatagttttt cgattgcccg cagattgcag tatgttgcag caccgggtat
     7021 atgaacgcca gcagatatac catcaggagg gattgcaata aggtagtaga tgttaataca
     7081 aggtaatcgt tgttttgttt ttctccaacc tcctgcacga gcggtttcaa taaaaaaatc
     7141 ccgtaagtcg tgagatgagt tgaagctatg ctgaagcctg tcattttcga agacatcatt
     7201 ttcttcatga aacacgagat tcatccatgg aaaaccattc acttcagcgt tcctatcaaa
     7261 ataatgttgc gcatagacaa cgttttgttt caccgtagac ttggctattt ccaaatcctc
     7321 aaatgcaccc ttctgtacaa taacatttaa tgagaaatgt gcggtttttc tcctcgttat
     7381 agagttagac attgttgatc gctcgtttct tcctggactt ttttgtacgc ttggcaaata
     7441 cccaaattga ggaaatgaag tgacgaacaa gttgttccga gaatatcttg cagcaagacg
     7501 aattgaagtt ctctaactcc cctggtagta ggttttattg atcatacgtt gggagaatta
     7561 gggttattaa gtaatgtcct aggacatcaa aaagggagga aaacaagtga gtagtgattt
     7621 caggagagat tttgaaagta tggaagtaag caggatcgat gtaagcagca tgttttataa
     7681 tagtagtgat tctggggatt tataccaata tcgatgcgga gacagatgcg gttgtagaga
     7741 cagatgttat gacagatgcg gttggagaga cagatgttat gacagatgcg gttggagaga
     7801 cagatgcggt tgtagagaca gatgcggttg tagagacaga tgcggttgta gagacagatg
     7861 ttatgacaga tgcagacgca gatgttggga ctgatgttga gacaggcgtc taatgggtaa
     7921 gatatagata tgctgataga agggccataa tccatgatta tcgtgggtta tggctttttt
     7981 tcttccccat cattttttga caagaaggac aaattgcaga gcataagatc aaaatagttg
     8041 tgaaagacat atttccgaaa tcaggtcacg ggaggaggca taaaatgaca tctgtcaacc
     8101 catccatgcg actaaaagta aaaggggata cgtattacgt tcctaatcca gatggccgtg
     8161 tgtattttca aaacaatcta ggatcttttc agatggaagg gaagggaatt cacaagtgga
     8221 ttgaaaaact gatgcccttg tttaacgggg aacatacatt ggaagaattg acaaatgggc
     8281 tgtctgacca gcgtagaaat cacatatatc acattgcaga catgctgtac caaaacggct
     8341 ttgtccacga ttggagcagt cagcttccac accagttatc caaggaaatt atggagaaat
     8401 acgctcctca gatagaattt ttgaacagct tcactgattc cgctgcctat cgttttcagt
     8461 tgtaccgtca aaccaaagtg ctggcggttg gttctggtcc cctactttct gctttggttg
     8521 cagcattgtt ggaatcagga ttgcctgcca tatacatagc cactacccat tccattgaga
     8581 ccaatcggga acgtttgtgg gaattggcag cacatgctcg aaaagaggac tccgaggtaa
     8641 agctggagga aatttacctg caaaaaaatg atcgggatgg ttggcagaag gctgtgcaaa
     8701 cttttcaagt tgtcttatat gtacaacaag aaggcgacct tgaggaattg cgggtgcttt
     8761 attcgatttg caaggaacaa aacaaaatct ttattccagc tatttgccag gaccaagtgg
     8821 gaattgcggg gccagtttta cgtccagatg cagatggatg ttttgaatcg gccaggcgcc
     8881 gaatccaccg atctttattc aacaacggct cttgtttcag cacttattcc cccatttccg
     8941 ctgccatgtt agcaaacatc gttgtgtttg aattgcttaa gactattaca gggacatctg
     9001 ggcaggaaaa taaaatcttt ctcatgaatt tggagacgat ggaaggagag tggcatccgt
     9061 ttacccctca tcctctggtg actggtggtg cacgtgtgaa atgggtgcag gacacgaatc
     9121 tactttttga acaacgtctt catcaaaatg gctcatatga tttgcaagca ttctttggtt
     9181 cgttaacatc ggatcaaaca ggaatttttc atacttggga cgagggggat ttggaacaac
     9241 tcccattggc gcaatgccga gtggtagtag ttgatccctt atccgaaggt ccggtagagt
     9301 tacttgctga catcgtttgt tcagctttga cgcatgatgg agctaggcgt gaagcgggat
     9361 tggcgggaat tgaagcgtat gcatcgagaa tggctaaacc gctatttggg actgaggaat
     9421 ttgttggaat aggcgcgggt gagacagttc cggaagccgt tacccgagga ttgcaaaaga
     9481 gtttgacagc agagctgcgt aaacagctcg aaaaccacca aactgctgtt tatccagttg
     9541 agctgaactc aatagaagat aaacattctc ttttttactt acaggctctg acaatcatga
     9601 acggcccacc gacggtatgc ctgggggagg aaatatcagg cttcccggta gtctggatag
     9661 gcacaaatga tcgctggtat tacagcgtat gcttacatct gactatggga ttgcgcaccg
     9721 tattgcagca ggcgttatgg gtaaaccata actcatctct tagccaaaca acgacatatg
     9781 ggattaatcc gtcctccatt gtagtaaaag aaaagcttcc acacagtttg gtcatcccca
     9841 cagttgaaga ggcagcacac tccgaaatgt tgcaagcggc cttgtcctct ttgagacgaa
     9901 acaataaaga gctgttcgtt tgtgaattgg aattggagca agtcttcaaa gaggaattgg
     9961 caggagtatt tggtgtgctg ttgcgaaagg atgaatcctg atgagcacag tcctgatgat
    10021 tgtcggagag ggattgttag cggatttcgt atatcgaaac ctgtcctgtc aatacagtgt
    10081 cgtgcagcag cctgattgta aggctgatat acccaaagag gtggaattgg ctttagtact
    10141 gcatgatttc tggcatcctt ccattcatct gcaggctgaa gaggtgttca agaaggctgg
    10201 cattccttgg ttaaggggct ttgtttcatt tggagagggc gtagtagggc cactcgtggg
    10261 accacgtatg caaggatgtc cgcagtgtgc cgacttccga agactgatga cccgcaaaaa
    10321 ccggagagat ctgagggaaa ttcaactcca gctaaaaaat caaaagaaac gacagcgtga
    10381 tgcatgggca tcaaatacgg gaatgtacca attggcgaac atgatcacag cggaagcgca
    10441 aagagtgtta cagggtagtc gcccatatac agtacaaaga acaagaatcc atttggtaaa
    10501 cttgcaaact ctggcgacct cagagcactt ctttttgcca aatccaatat gtgaggtatg
    10561 tggggaattg cctgaggata cacctgaggc cgcacgtatt actcttcaac caagaccaaa
    10621 ggcaaatccc aacagttatc gaatctgttc gctagatgtt tttaaggaag tcctcattca
    10681 ggaatatctc gattttcgaa ctggcatgat caaccaaaaa accaaagacc acctgacccc
    10741 atttgccgtc atgagcgtga atctgccgat gataacgtct aatgaaggag cagccgggcg
    10801 aactctttct tatgagcaga gtgaactgac agccatcctg gaggcattgg agcgatacgc
    10861 aggtctgcat ccacgaggca aaagaacggt catctgcgac agttatgaga aggtgaaaga
    10921 ccacgcactc aacccgctga agatcgggct gcatttggag gaacaatatg caagtcctgg
    10981 ttatcctttt cagaggtttg atccaaaacg gcccatgaat tgggtatggg gttattcatt
    11041 tttcaaggag aagccaatct tggtaccaga atcgattgcc tactacgccc tggaagatga
    11101 cttcgtctat gaaaactcca atggttgcgc gttaggcagg tgtttagagg aggccatttt
    11161 ctacggtata ttggaagtgg tggaacgaga cgcatttctt ttgacatggt acgcacaact
    11221 gcctttaccg cggcttgatc ctgaatctgc gaaagacaaa gaattactct tgatgattga
    11281 gcgaataaaa gcagtggcag gttacgattt gtatttgtat aacgcgacaa tggagaatgg
    11341 gataccaagt gtttgggcca ttacgaaaaa caggaaacag aagggagtca atctcgtctg
    11401 tgccgccggg tctcatccgg atccgttgcg agctgtaaaa accgcggtgc atgaactggc
    11461 cgacatgctg ttaacactgg atgaaaaata tgaaacgcat cgggaagagt ttctgcaaat
    11521 gctgcacgat cccttccggg tgcaaagcat ggcagaccat ggtatgctgt acagtctgcc
    11581 tgaagcggaa gagcgactgc actttttgct tcatgatgat cggcccatgc gaacatttga
    11641 cgaggagttt aagctgcagg caggtcatca tgacctgacg gatgatttga aagagattct
    11701 gcagattttt aagcaattaa acctcgaagt gatagtggtt aaccagacga caccagaaat
    11761 gatcagaaac gaactgtaca gcgtgaaagt tctgatccct gggatgctgc cgatgacgtt
    11821 tggctatcac ttaacccgct taacaggact ggatcgtgtc ttgaacgtac ccatgcagct
    11881 tggctatacc aaacatcccc tgacatatga acaactgaat ccgcatccgc atccatttcc
    11941 ataggtcttg aagtaggagg aaaaagtatg cgcagggatg tatttttgca caatctgctg
    12001 tatgacatcg aaaaaattat ccccgccgat tggacagtag aatgggaaga ttcccctcta
    12061 gtttataaga tctatcgcga actgcctgtc gtgccgcttt cgttagaagt tcggctgtct
    12121 cttgaacacg ggagaaataa acaatcatcg aagccaacca ttcgtgatat tggccatttt
    12181 ctctgggtca catatggtct tacccgagtg tctgatccag gtaatgactg gaattccttc
    12241 gaacaaagat cagaggagtt attccgaaga tttgttccgt ctggaggggc tttgtatcca
    12301 agcgaattat acgtgtattt aaacatggat gacttgcccg tgggagtgta ccattacgac
    12361 gctgcccatc atcgtttggt gttgctgcgc aaaggaaatt gcgattcata tctgacaatg
    12421 gctctgggtt acaattgtga catgtcagag tgttttgctg cagtatttgt atcgacaatg
    12481 ttttggaaaa atttctttaa gtatcataat ctgtcgtacc gcctccaagg tttggatgca
    12541 ggggtactga ttggccagct gcttgaagca gcgaaaaggt ttggttttac aacaggtgtt
    12601 tactatcaat ttctcgatcg agccgtcaat catctgctgg ggctgaacca atgggaagaa
    12661 agcgtatatg cggtgattcc tctctcgata aaatcagata tcaattggtt tgcccataag
    12721 cgtgaaggaa tggaaattgt ctgggataaa gaattgtatc ggaagttacc cgctgttcag
    12781 catcattcct atatccgttc tcgaaggatt agaaaatatc ctatgctgat aaaattgaat
    12841 gaagcatcaa agttcgaatc aacggactgc tttcatcggg tacggggaga aaaaaagcct
    12901 gtctttggag gtccttcttt ggcattacca aacgtaaaac ctttaaactg ggatctcatg
    12961 gaggcaagcc gaaagaggtt ttcacctggt gagcacttcg ttttacgcga agtgagtcaa
    13021 cagcaattag ccactctttt gcgtgaggcg acaaatgctt tcttctaccg aaatgatctg
    13081 gatcgaatag atatccccga aatccaagaa caaccgaggt ctcgagtttc gttgtactgc
    13141 tgtctttatt ctgtggagga tattcccgct ggcgcctatt attatgacta tccttcccat
    13201 aaattacggc tcattcgtct tggagaccat cgccccatat taaaacaggc aatcaatagg
    13261 agcgacttca atatactcga tgttccgatc tgcctgcatg tggtagggga ctatgattac
    13321 tacagacata aactcggata cagaggatat cgtattcaac aaatggaagc gggtatactt
    13381 gtacagaaat tactgttggt agcttcttcg atcggattgg caggccatcc gcttctcgag
    13441 tatgatgcaa aaacatgcga taaaatctac aaaatcacga atgtaaacca aagctgttta
    13501 atacaagtac ctgttgggcc ctatcgttta cccattaggt taaccggacg tttgtacata
    13561 taggaaggag ctggagcaca cttaaaaaga catcaattac gcagcgaccg tttcccgata
    13621 ttccactggg gagcggtcgt ttcgttttcc caatgaaaaa atccccctcc gatcacaacg
    13681 tctaaatcca tcttcagcag atgccttttt tacgttttct accgttaggg gatattaccc
    13741 gaacttatct cccggttcag gaacggatac cccatggaga gcatgttcgc tcctgctgcc
    13801 tgcatcgagt gcgcgaagtt cttccaaggt gtgatccagc tgctgcgtgt agcgcggggg
    13861 caaattacga atcatccgta tttgaaaata taaaaatttt aggaagcgat aggtactgtg
    13921 ttatactgag cctaccattt actgttcttg tagcataccc tactccaatt acgtgagtag
    13981 ggttttttat ttgtgtttca gacaacaaaa aaacagaccc ccatacagga acctgttttt
    14041 tgtgtaaagc tatactaaac tttttgaacg ttagtagctt gtggtccgcg ttgtccttgt
    14101 tctacgtcaa aagtaactct ttgaccttcg tcaagggatt tgaaaccttc gctttggata
    14161 gcagagaagt gaacaaatac gtcttcttta ccttcgcgct cgataaaacc aaaacctttt
    14221 tctgcgttaa accattttac tgtaccttgt tccattgtta ttgcctccta gtgtgtaaca
    14281 cacatatatt actatccttg ctctcaggat catcaagacg aaatttaata tctacgctat
    14341 ccgttgcttc gaacaaaaat aattctctac tagcataaca ggatattgaa aggatagcaa
    14401 atgattcacg aatgttgacg aaagaaaatt cagaggagcc cctatactaa ctccaacctg
    14461 cccctatttc acaagctttt attcagatca gtcaattctt tggccgtcag attgcgccat
    14521 tgacccaatt tcaaattccc cagcgtaatg ttcatgatgc gtatccgttc aagctttcgc
    14581 acctgataac caaaagcttg gcacatccgt ctgatttggc gattcagccc ttgcgtcagc
    14641 acgatattga agacgcggtc gctgactttg gttactttgc aaggctttgt agtcgttccg
    14701 agaatgcgta cgccgtttgc cattccgtgc aagaaattcg ctgtgatcgg cttgtctacg
    14761 gtgacgatgt actctttgtc atgattgttc tcagcacgta aaattttgtt tacgatatcc
    14821 ccgtcattgg ttaacaggat gaggccttgc gaatctttat ccagacgccc gataggaaaa
    14881 atccgttccg ggtgattgac gaaatcgatg atgttgcctt tgacgtgcag ttcagtggtg
    14941 cacgtaattc cgacaggttt gttcaaggcg atgtagacat ctttcttttt agcaccgagt
    15001 ggctgtccat cgatgcgcac gtcatctccc aacgcaaccg tacttcccag ttccgcgagt
    15061 tggccattga tcgtcacgcg cttggcctcg attaatttat cggcttctct tcgcgagcaa
    15121 atgccggttt cactgatgaa cttattaatt cgcatgagac gttcctttcg ctattactgc
    15181 tgcttttcca gtttttccag tagtttattc cgccaaggca gggccaattc ctctacctcg
    15241 agcatacgct gtatcatctc ttgggatggt gtcttgtcgt ggaagtataa gtggtgaatc
    15301 gacgctaccg ttacttgttt tggatgtgca gtgatgaggg taatcttaga gtctggcttg
    15361 atcgtccctt cctgcaagac acggaagaaa tagcccgtat agcccaattc caccaccgct
    15421 ttgagcagtt ggttattgtt gtttcgtttg ttgatcgtcg cgcaaggaaa tctgccttgt
    15481 gtcacttgca ggatggtatc accgaattga tacacatccc cgatgcagac ctgctcttcg
    15541 gtcatgccag cgagcgtgag attttctccg aatgccgcat tagtcagagg gtggccgaat
    15601 aacttctccc aatgggcata atgttcaaaa ggatacgcac agacgacgcg gtctgggccg
    15661 ccgtggtatt ctggattcgc gatatcgtcg tcatcaattt tctcggaacg gactatgagc
    15721 tcgtttgctt cctccttcca aatgccggag agatactcct tgccgttgta catattactt
    15781 ttaggcaaac ctttactaat tgtaacgatt tttctgttca actaatgctc actcccagcc
    15841 tgtacgattg cttctctttt tatccccata ctataccaca ttccctaata tcctccacag
    15901 cggatgggca ccagcggacg cttctggtat gatggtagaa aggaatacac aggaggcctc
    15961 agtgacatgc aatcatttgg tgtggcacta cacacagata aatatcaaat caatatgatg
    16021 tacgcccatt gggtgaacgg aacacacaat cagaaggtcg ttttcgaggc atttttccgc
    16081 aaactgcctt ttggaaacgg atacgccgta tctgccggat tggaacgaat tgtttcctat
    16141 ataaaaaatt tgcgatttga agaaagcgat ctccagtatt tgagtgagca ggaggagagc
    16201 tacgatccga agtttttgga agagctgcgc cagcttcgtt ttaagggctc actctactcc
    16261 gtgaaagagg gaaccgtggt tttcccgaac gagccgctga ttcgcgtaga ggggcgtgtc
    16321 atggaggcac agctggttga aacggccttg ttgaactaca tgaactacca gacgctgatt
    16381 gcgaccaaag cctcgcggat taaaaacgtg gcgggcaatg atgttcttat ggagttcgga
    16441 acaagaaggg cgcaggaggc agatgctgcg ctatggggga cgcgggcagc gtatttggct
    16501 ggctttgacg cgacctccaa catgctggca gggaaaatgt ttggcattcc gacaaaaggg
    16561 acgcacgctc actcatgggt acagagcttc gagagtgagg aagaagcatt tgagcgcttc
    16621 gcagcagctc ttccgggaca ggtaaccttg ctggtcgaca cttacgatac gctcaaaagc
    16681 ggcgtgccga atgcaattaa ggtcggaaaa cgcctggaga aacaaggcaa aaagctgacg
    16741 gcggtgcggc tggacagtgg cgacttgacg tatttgtcct gcaagacacg cgaaatgctt
    16801 gataaggcag gcatgaccga tgtgcaaatt gtcgcttcca acgatctcga tgagcacacg
    16861 attatgaacc tgaaagtaca aggagcacgc gtaaatagct ggggagtagg aacacagctg
    16921 atcacggcag cggatcagcc gacgctcgga ggcgtataca agctggtggc gcgcgaaggc
    16981 gctgacggaa aatatcagcc gacgatcaaa atctccggga atccagaaaa agttacaacg
    17041 ccggggatca aagatatgta ccgattgatt gatccggaaa cagggaaagc gattgctgac
    17101 tacatgtgct tcccgcatga aacaacagtc gatgatagag cacctctcca tttgttccat
    17161 ccgagccata cgtatttgag caagcatttg gagaactatc aggcagaatc gctgttagtt
    17221 ccagtatttg tggatgggga actggtctat gaactgccga ccctccaaga gattcgcacc
    17281 tatcatcgtg agcaactcaa gctgttctgg cctgaatact tgcgcatgct gaatccggag
    17341 cattaccgtc tgcacatcag tgaagagatg tgggagacga aaacaaagct gatgaaggcg
    17401 tatttgaaaa aataggaaaa aacgtctgat gtgtcgcgag gatgcatcgg gcgtttttta
    17461 tttgcttacc aaaataacat aataatgtga aaacaaagaa aatgaattgt ctgaatttgc
    17521 tttcatcgta tataattact ccattcatcc tttagctctg tgccaaggtg atgtagaaaa
    17581 cgagtgtgaa aagtattggg gaggaagaga gatgctaaat actgtagaga aagaaaacct
    17641 gaatccatac gaaattgtgc agaaacagat tgatgctgcg gcggcattgt tgggattgcg
    17701 gagcgatgct gtagaaattt taaagcggcc caaacgagta ttggccgtca gctttcctgt
    17761 caagatggat gatggcagcg tgcgtgtctt tgaaggctac cgatctcagc ataatgatgc
    17821 ggtaggaccg accaagggag gaattcgctt tcatccggat gtgacgatgg atgaagtcaa
    17881 ggcgctctcc atgtggatgt cattcaaatg tggcgtggtt ggtcttcctt atggcggcgg
    17941 aaaaggcggc gtcatatgtg atccccacga attcagcaaa aatgagctgg aacgagtcag
    18001 ccgcggcttc atggaagcca tcgctgatat tgtcggacct gagacagaca tacctgcacc
    18061 cgatgtgtat acgaccccac aaatcatggg ctggatgatg gatacgtaca gtcgattaaa
    18121 aggcacctat tctccgggag tgatcactgg aaagccgctc tctgtcggcg gctccaaagg
    18181 aagaaacgaa gcgactgccc gtggatgtgt ctttactata ttggaggcgc taaaagattc
    18241 tggacgcaag ccggaacaaa caaccgtcgc cattcaaggc tttggcaatg cgggaagaat
    18301 tgctgcaagg cttttaacag aactgggctt caaaatcgtg gcggtcagtg actcacgcgg
    18361 aggcatttat gatgctgctg gcttggacat cgaaaaggta ggacagctaa aagacgacgc
    18421 caccattttg gagtatgtag gtggtacggt catctccaat gaacagctgt tggagctcga
    18481 agtggacatt ctgatcccgg ccgcgttgga aaatgtcatc acagcagcga atgcccacag
    18541 cattcaggcc aagtggattg cagaagcagc aaacggacct actacccccg atgctgacgc
    18601 cattttgcgg gaaaaaggga ttaccgtgat cccggatatt ctcgccaatg cgggtggcgt
    18661 aactgtttct tattttgaat gggtgcaaaa cctgatgaat tactattggt ccgagcagga
    18721 ggtaaacgaa aagctgcaaa cgaccatgat taacgcttat cgcgctgtga aagaactctc
    18781 tgatcaatac aaggtggact tgcgcacggg agcttatatg atctcgcttt tgcgaattac
    18841 ggaggcgatg gaagcgcgcg gctggattta agggaagaac ctcttgcaac ttgcttgcaa
    18901 ggggattttt tcgttttccc ctgcgggatt agcaggaagg cgagtatttt tgtcgaattt
    18961 gtcggattat tgtttttcta cctgtgttca aaggatggaa agggggatct ttatgccaat
    19021 caaattgccc gacgaattgc ctgcaacgga aatactcgcc caagaaaata tttttgtgat
    19081 gaaggatagc cgtgccttca cgcaggacat acgaccgctc aggatcatca ttctcaacct
    19141 catgccagtg aaggaaacaa cggaaacaca gttgctccgc ctcctgggga atacgccgct
    19201 acaggtggag atcgtgcttt tgcacatgtc cagtcatacg tccaaaaata cttcggaaga
    19261 acatttatcg atgttttata aaacgtttga ggagatccgt gaccagcgat ttgatggtat
    19321 gatcatcacc ggagcgccag ttgaacagct cgagttcaca gacgtcacgt attggcagga
    19381 gctgacggaa atcctcgact ggaaaatgga gaacgtcact tcgaccctgc atatttgttg
    19441 gggagcacaa gcgggtctct accaccactt tggcgttccg aagcatcctt tgccagaaaa
    19501 aatgttcgga atcttccctc atacactcaa taagcaaaat gtgaagctgc tccgcggatt
    19561 tgacaactac ttccacatcc cgcattccag gcataccgaa aatcgcaggg aagacattga
    19621 gcaagtgcct gaattggaga ttttgtccga gtctgatgaa gcaggggttt acatcgtagc
    19681 gacgagggat ggcagacaaa ttttcgtgac aggacattcc gagtatgatc cgaccaccct
    19741 gcaagatgag taccagcggg atgtgaacaa aggcttggat attgctgttc cgcgtaacta
    19801 ctatccaaag gacgatccaa gccgcgagcc catcgtcacc tggcgcgcac atgcgaattt
    19861 gatgttctcc aactggttaa actattacgt ttaccaagag acaccgtatg atttgaacaa
    19921 cgacaagcga tagcaacgta gcagccatcc gaggtgattc ggatggtttt tgtttggccc
    19981 agttgacgta ataaatggcg acaggtagtg tgagaataat cgcgcaggaa tactaccggg
    20041 aattcaagca agatgggagt ttgtatgatg aaaaaaatca tgaaacaaga tcaggacgtc
    20101 accaagcagc ataacaagca aatgatcctc gacattatca aaaagcagag accgatatcc
    20161 cgtgcagaaa tcgcaaaaat cacgcggatg agtgccacct cgattggcag atttgtcagc
    20221 gagctgtgcg aggaaggact gattcgggag acagagctta cctcatcagg tgtgggcagg
    20281 aaggcaatca tgctcgacat cgagccgggg gccatttata caatcggcgt ggacatggca
    20341 aaaaagcgca tcggcattgg gatgccaggg gtgatcaatt atgagcgagg gattgttcag
    20401 ctatccacta cgttggactg gaaggacgta gcgttcgcca acctgattga aaaaaggctg
    20461 aaaatcaaaa ccgttatcga caacgatttg aaggtgaaaa tcctctcgga gtacttgtac
    20521 gggtccgcta aaggctcgaa caagacagcc ttgatcggca ttggcacggg tatcggttct
    20581 gcattgatta tcaatggcga agtgtttcgc gggggctcga atagtgcagg agagctgggg
    20641 catacgacgc tcgatccgaa tgggaatctg tgcgaatgtg gaaagcgtgg atgcctgcaa
    20701 acatacatag acgaaaattc tttgatcatg gaagcgcgtc gggtgaagga tgtcaatgat
    20761 gaccgacagg tgttcgcggc ggcgagaaac gaagagcgct gggcgaagga aatcgtctcc
    20821 cgtactgcat tgtacctcgg aatcacgatt aacaatattg tatgcatgta caatccagat
    20881 accgtgattg taagcggtga tttggtggag aattatcgcg aaatcattga cttggtcgag
    20941 gagcatagcg atcaagtgat ctgggagcca ttcaagggga aatttcgcgt cattccttct
    21001 gaattgaagg gggaggcgat cgtgatggga gctggagctt tggcgatgca gcacttcgca
    21061 cccttggcag ggagatgggc agaagaagta tgactttctt tgccattttg tattcaacaa
    21121 gtatagttgt ttagccgata tagaaaatgg tagagaatta gagaggagag ggatgtgagc
    21181 atgagaaact ggcaaaaagg gatgctcgct acggctgtta ccgttgggct gatcttttca
    21241 agtgtcggcc aagtaccggc attggcgaat tctgcgacga caagtacgca gaagaagatt
    21301 tccaagcttg tggttgatac aaagacagta aaattgaaaa aaacggggac acagcagtta
    21361 aacgtccgcg tgcaatacac ggataaaaca acggaagacg tcacacagca agcagagtgg
    21421 acttcctctg acagtgacat cgcaaccgtt gacaagggct tggtaaccgc tgttagttcc
    21481 ggaaaaacaa gagtgaaagc aagcttcggg ggcaaaagtg tgaatgtccc tgttgagatc
    21541 gatgtgattt ccaagctctc tgctgaccag aagaaactgg ctctcagcgt gggaggcacg
    21601 aaacaggtaa acgcaacagc aaccttctct gacaagtcta ctgctgatgt aacggctcag
    21661 gcagagtggg aaagcgatga tacggaaatc gtaacggtca cgaatggatt ggttacggca
    21721 gtaggctcgg gcaaagcgaa gctcaaagtg aagtacggtg gtaaaagcgt aacgattcca
    21781 gtagaggtgg atgtcgtctc caagctggac agtgataaga aaaagctgta tctgcgtcca
    21841 gacactaccc agtcagttac actgctggct accttgtcta ccaaagaaaa agtcgatgta
    21901 acaagcaaag ccaagtggac atccgacgat gcaaaagtcg ctacagttga aaacggagtc
    21961 gtgacaactg taggctccgg caaaacgaga atcaaagcaa cttacggtgg aaagacgatc
    22021 tcgatccctg tagagtctgc tgtcatctcc aagctggaat ttgacacgaa aaaggccgat
    22081 ctcaaagtcg gcgaatcaaa ggacttgaag gtatctgttg tctataccga caaaaccaag
    22141 gttgatgtca caatcgatgc agactggatc tccacgaaca gctccgttgt gactgtagcg
    22201 gatggcaaga tcacggctgt gaaagaaggc cgtgcaacga ttgtggctac gtacaatgga
    22261 aaagttgtaa aaatacaggt aaccgtgaag taactcacgc tggaggaaaa gccagggcgc
    22321 gactgcgtac ctggcttttt cgcatactct cgcctgtggt aatctgtagc taccactttt
    22381 gattggacga agacggaggg catcatggat attcgacagc tgcattactt tgtggaagta
    22441 gtcaaacata aaaactttac gaaggccgct caatccctcc acgtctctca gccttctatc
    22501 agcaaaatga tcaaggcatt ggaagaagag ctagaggtag ttctgctgga tcggacggag
    22561 cggaaaatgg atctgacaga agcaggggaa atggtctaca actatgcgac caaagtgttg
    22621 cagctgatgg aaagcatgtc gtcgtcgatt gcagaattgc gaaatgtcga gcgtggtcgg
    22681 gtcaaactgg gcatgatgcc aacggtgggt tcgtttctct tgccgaatgt gattgccttg
    22741 tttaaaaagc agtatccagg catcgatatc gagatgaagg aatacagcgc caagctgctt
    22801 gaaatccaaa tggagcaagg aagcatcgat gtggcattaa ccgttcttcc aacggatcag
    22861 gagaaatttg tggcggttcc cttgctcgca gaggacctcg tcgcgatcgt tcatcgggag
    22921 cattggctcg cgggagtcga tgaggtgagc ttggaacagc tcaaggacga ggctttcatt
    22981 ttgtttaccg aagagtacgc gatgcacgat gtggtgcgcc aagcgtgcaa gctgtccggc
    23041 tttgaaccga aagtcgctta catgagctct ttatgggatt ttgtcgggga gatggtggct
    23101 acccaactgg ggatatcact cattccacgc tccatcgtga gacgcttgaa tcacggacaa
    23161 ttgcacaccg tcaacatttc ctacccggtg attgactggc agtatgcctt gatttatcga
    23221 aaagaagggt atttatcgca tgcgacaaag gctttcatat cgtttgtaga gaaaatgtac
    23281 agccataacc aaatggaata gaaatgatga tttttatgta ttgtacgaat ggaatcgaga
    23341 cggcttagaa tgaaggtaac tcgttcaaac agtaactttt tgaacaacct ttgtagagca
    23401 actggggtgg tctcgatgaa aagatggttc tccatcatcg cacaagtttt gcttttgttc
    23461 ggcttcacct ggcttgggaa atgggtaagc tcgttttttc accttcctgt tcccggcagt
    23521 ctgatcggac tcgcgctttt gttcatttgt ctatatactg gct
//
