LOCUS       NZ_CP018145            22361 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Brevibacillus formosus strain NF2 chromosome, complete genome.
ACCESSION   NZ_CP018145
VERSION     NZ_CP018145.1
KEYWORDS    RefSeq.
SOURCE      Brevibacillus formosus
  ORGANISM  Brevibacillus formosus
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Brevibacillus.
REFERENCE   1  (bases 1 to 6437015)
  AUTHORS   Fu,B. and Li,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2016) Insitute of Enzyme Engineering, Shaanxi
            Academy of Sciences, No 16, East Renmin Road, Xi'an, Shaanxi 710600,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Coverage                   :: 167.68
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 02:46:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,168
            CDSs (total)                      :: 5,986
            Genes (coding)                    :: 5,821
            CDSs (with protein)               :: 5,821
            Genes (RNA)                       :: 182
            rRNAs                             :: 16, 17, 17 (5S, 16S, 23S)
            complete rRNAs                    :: 16, 17, 17 (5S, 16S, 23S)
            tRNAs                             :: 127
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 85 of 165
            Pseudo Genes (incomplete)         :: 95 of 165
            Pseudo Genes (internal stop)      :: 22 of 165
            Pseudo Genes (multiple problems)  :: 35 of 165
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:35:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4761844
            Orig. end                         :: 4784205
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018145.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22361
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lanthipeptide-class-iv"
                     /region_number="17"
                     /rules="(cds(LANC_like and Pkinase) and not micKC)"
                     /tool="antismash"
     cand_cluster    1..22361
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(LANC_like and Pkinase) and not
                     micKC)"
                     /kind="single"
                     /product="lanthipeptide-class-iv"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22361
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12361](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(LANC_like and Pkinase) and not
                     micKC)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-iv"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12361
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(LANC_like and Pkinase) and not
                     micKC)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-iv"
                     /protocluster_number="1"
     misc_feature    complement(294..308)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.65"
                     /tool="antismash"
     misc_feature    complement(379..394)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 21.73"
                     /tool="antismash"
     gene            548..964
                     /locus_tag="BP422_RS22565"
                     /old_locus_tag="BP422_21985"
     CDS             548..964
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018885325.1"
                     /locus_tag="BP422_RS22565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_21985"
                     /product="hypothetical protein"
                     /protein_id="WP_233220522.1"
                     /transl_table=11
                     /translation="MFMGKGTVRELGNQIDKVLGDIKDIQAEIDRDSDKIDNELNSCSR
                     ELINAQTTLGEIQPLIESLVAQVGQNAPDHIKVLVGTIADGITGKVKNTLNNLAEVQKN
                     VKDVDKLTDAIDGHTDKIAQKVKEIDSITDKVQK"
     gene            973..1299
                     /locus_tag="BP422_RS22570"
                     /old_locus_tag="BP422_21990"
     CDS             973..1299
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BP422_RS22570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BP422_21990"
                     /product="hypothetical protein"
                     /protein_id="WP_088909596.1"
                     /transl_table=11
                     /translation="MSYINNSNGWGTQHTNHVANKVDHLADKISFAVDMVANAAINLDG
                     AQQELLTLANAPYPPQSTQLITLANRIAANKRQIDDGLAKIKEMARTIDQETNQIQTPP
                     QKGW"
     gene            complement(1361..3682)
                     /locus_tag="BP422_RS22575"
                     /old_locus_tag="BP422_21995"
     CDS             complement(1361..3682)
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251698.1"
                     /locus_tag="BP422_RS22575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_21995"
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="WP_088909597.1"
                     /sec_met_domain="Peptidase_S9 (E-value: 4.7e-38, bitscore:
                     129.2, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENVVTWEQYERAQRLHPANYYDAVFNAFVRPRWISGSQFWYIRE
                     IRLEKGSGRQYVIVDAEQNTSQPAFDHERLAVCLSGSAGKGYDAETLPLGELMFTESGS
                     AFQFEVGGTVWSCDLQEYTCKPLKNFKKPSMDELPSPDGKWVAFVYDHNLYVRSLETGR
                     RIRLTYDGKAYYDYGTQPECRLSIMAERLNGTKLPPVAIWSPDSKMLLTHRLDQRKVRE
                     MDLIQSVPKGEGVRPVLHSYRYPLVGDQHVPLAELVICNLERRTTVRIESEPLMACPVS
                     PLTQGMQMAGWSSDSKQIYIARMSRDARSVDFSLADPCSGEVCTLLREQSDTFLNQDLH
                     NLGASNPNFRLLADGSFIWHSERDGWSHLYYCDGKTGEIRNPITSGSWTVRRLMAVDEQ
                     QGWVYITGGGLEEGRDPYYQHLYRVRLDGTGLSLLTPEDAEHDVVFSPNLKYFVDTFSR
                     VDLPPVTVLRSVDGSLIRELEYADVEMLLEMGYQFPERITVKARDGVTDLYGVMLRPFP
                     FDPSRKYPVVDYIYGGPQIINTPKAFALDPGRSHDPLGGGQSLAQLGFIVLILDGMGTP
                     YRSKAFHDISNGNLEEAGGLLDHVIAIKQLAERYPYVDVDRVGIWGSSGGGYASTRAIL
                     SFPEFYKTAVSACGNHDQRLYIAAWAERYQGLYDPDLYRDQDNARLAANLEGKLLLVSG
                     DLDGNVHPSQTIRMANALIKADKDFDMLILPNRHHGFSLEPYFIRRKWDYFVRHLMRAE
                     PPWGNQKSYKNTSDKESMEK"
     misc_feature    complement(3634..3647)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.31"
                     /tool="antismash"
     gene            complement(3886..5478)
                     /locus_tag="BP422_RS22580"
                     /old_locus_tag="BP422_22000"
     CDS             complement(3886..5478)
                     /EC_number="2.7.7.65"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025: diguanylate cyclase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015893437.1"
                     /locus_tag="BP422_RS22580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_22000"
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_088909598.1"
                     /transl_table=11
                     /translation="MPVLQKRANHPKKISLVTLLTGLVSLSVVLTVTILLLSFYQSKKQ
                     ALINTTLTLNHTSAIKMSHTLDALIKSMRSSLQYSASIFSNRKEMNADEVYSKLELIRR
                     TNNYFNSVSVVDETGLVRGVSPGSIGTVGSYVKTKTGKTALALKKPYISEPYVTASTKR
                     LIVLMSEPIFDKDGFYRGIIGGTIYLHEDNILNTIFGSNPTDELGSSFYIVGSDGHLLF
                     HRDTNRIGEDISANPAVRKLIQGKSGYEQIVNLRGQTVLAAYVKVPANDWGVVVVSPIS
                     VVQEQLNRDIQSILLYMLPPFLLLMLVVIGLARKVAKPFVSLANLVSKAGREEVELSEG
                     GQHWNREAELLSHAIRFALTNIKKQTEQLAHDARTDPLTGLTNRRTLEETMQEWMAEQI
                     PFSTIMMDLDRFKAINDTYGHPAGDEVLRHFAKIISSLLRPDDVSCRFGGEEFVVLVSH
                     AGLEEAYQVAERIRHALETRTNPIGQSITVSQGIAHYPSHAVSPEGLLQLADEAMYKAK
                     RSGRNQTMIAEAE"
     gene            complement(5646..6293)
                     /locus_tag="BP422_RS22585"
                     /old_locus_tag="BP422_22005"
     CDS             complement(5646..6293)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015893438.1"
                     /locus_tag="BP422_RS22585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_22005"
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="WP_088909599.1"
                     /transl_table=11
                     /translation="MIGHTTEKERTIFDHTGNRIKTEDREIQILAKYEEPLVVVLGNVL
                     SDSECEELIEHSRERLQRSKIGEDRSVHSIRTSSGMFCEQMETITRIEKRISQIMNIPI
                     EHGDGLQVLRYTPGQEYKPHYDFFAETSRASTNNRISTLVMYLNDVEQGGETVFPLLHL
                     SVFPTKGMAVYFEYFYRNQELNELTLHAGTQVIHGEKWVATMWMRRQSFRVS"
     gene            complement(6503..7849)
                     /gene="brnQ"
                     /locus_tag="BP422_RS22590"
                     /old_locus_tag="BP422_22010"
     CDS             complement(6503..7849)
                     /GO_function="GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /gene="brnQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017251695.1"
                     /locus_tag="BP422_RS22590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_22010"
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="WP_088909600.1"
                     /transl_table=11
                     /translation="MSQKAPVSFVVTIGFMLFALFFGAGNLIFPPMLGQLAGTNVWIAN
                     AGFLVTGVGLPLLAITAFVYSGKDDLRSLASRVHPVFGMVFTIVLYLAIGPFFAIPRSG
                     NVSFDIGVKPFLSEQAGSAPLILFTILFFAIACFLSLNPMKIIHVVGKILTPIKLTFIG
                     IIAVTAIVYPIGALQAPQADYTSHVFFKGFQEGYLTLDALVAFVFGMIIVNAIKEKGVT
                     ARKQVLATCAKATMIAGLLLALFYTALSFMGASSVEKLGHLENGAEVLAKVSNYYFGSY
                     GAILLGLMITVACLTTSVGLITACSSFFHSLFPKISYKKFAVILSVFSTLVANIGLTQL
                     IKVSVPILMTMYPIAISLLFLTFLHKAFNGRPEVYQGSLLFTFLISLFDGLKAAGVENS
                     VVHHYFTQYLPLYDVGLGWLLPSLIGGICGYMISVFRKNMYHVQKQKEA"
     gene            8402..8725
                     /locus_tag="BP422_RS22600"
                     /old_locus_tag="BP422_22015"
     CDS             8402..8725
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BP422_RS22600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BP422_22015"
                     /product="hypothetical protein"
                     /protein_id="WP_088909601.1"
                     /transl_table=11
                     /translation="MAFKSRNVMARVLGQGGDGGQVFPNCLITLAVPTPAQRRSGCVDA
                     HSLCQVVSVCNVVTVCNLTLNPQVLEDAGIEFLDPPKAQVSKEVRELKRQIERIFEEFE
                     ERD"
     gene            8825..9997
                     /locus_tag="BP422_RS22605"
                     /old_locus_tag="BP422_22020"
     CDS             8825..9997
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BP422_RS22605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BP422_22020"
                     /product="hypothetical protein"
                     /protein_id="WP_088909602.1"
                     /transl_table=11
                     /translation="MSFHLQQATLRANPAYKLVLYDRLPAEEQKALDELRHDPDFYGFL
                     VPIEGTLAMKAVCRETALLFLTLQNPGSVPTYVQKLYGGAWEDDLFELILDDVIEVEVG
                     GQFHSGAAAQALCGKSAPISKGHIGRLSMAALQYGEALGITKVPVLSRRLYDFNRLPAT
                     SEWHRMIPDHSALLAYAGIQPDGPVQSKLQRHWIAVAHSRANGWLTWTNQNPATAIRPD
                     SMIYKLYVSPHPSCLADALAAAIATFTECAVPNFKLGSDLYGILRPDKLIAYFSSLEEV
                     MAVADKLKLRLTGCPAQGVPFTGELESSGLLSWGMDPPQVPSIAWSPMESWRLWITNHL
                     ASALIAARLAPATGLSPWEFAMARIRLANVDPETWTPLPSLRWNLPDEEG"
     gene            10001..12361
                     /locus_tag="BP422_RS22610"
                     /old_locus_tag="BP422_22025"
     CDS             10001..12361
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-iv: Pkinase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-iv: LANC_like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /locus_tag="BP422_RS22610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_22025"
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="WP_088909603.1"
                     /sec_met_domain="Pkinase (E-value: 2.4e-20, bitscore: 71.4,
                     seeds: 54, tool: rule-based-clusters)"
                     /sec_met_domain="APH (E-value: 8.7e-07, bitscore: 27.5,
                     seeds: 156, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 6.9e-35, bitscore:
                     118.9, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRITDVFVLPDDVQIKAVSELPADLLGDLVCEEGEQAIIRLGTRM
                     PIRLLDEEGVKLLLHFRPAKTIGAAIIEYCRGNGGDPQSILEEAYPMLRLLIDSNLLVP
                     ADSPAARPVTPSFRPRESVAGCRVVRCLQWLTDSELYHVKDSNGVNAALKIARPEFAEM
                     MRPLFTHEAEVLRCLDGRVNPPLLAAGDLNGRPYLRIGWCPGEHVDTRAHNLRPLQTTL
                     GRQRLLKLSCAVLDAYTHLHAQGVVHGDVHPRNILISDDGQVSIIDFGFSRREIHKDSG
                     GMPNRGGIPYYYEPEYAWASLERRSPPHATQAGEQYALAALLYELITGKPYTNFSVEIE
                     SMLQQITKEPPLPFADQGVAPWPEIEAILCRALSKSPPDRYGSLAEFSSHMRTLLHCEA
                     VDHSPPAIPETAARIVFEVLDRVGPGGSLSFTDLTPPTCSVYYGAAGIAFALYRMACSR
                     GDALLLSQADVWTCWAESAITGTDAFRIPEAQLPPEWFCTVTPFHQASGVYLIRGLVSQ
                     ALGDPASMQRAIDSFVTACSTPCTNPDLTLGNAGVLLSCGMLLEAAPDIPGMDITNLLA
                     LGNQLVESLWASSITVPNSYLGIAHGWAGILYATLHWHRITKNPLPEGTLKALEWLAAQ
                     AKENGNRAWWPVTTDSEQAAQGWCHGSVGYVWLWTIAHHLFKQESYLQLAIQAAQYAWD
                     TPAVNGTICCGMAGQAYAMLHLYRYTGEKHWLQKAFALTEGAADSIQRVDERRDSLFKG
                     HLGVAVLAVDLEHPNRACFPLFEIEGWPGSQKV"
     misc_feature    12881..12895
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.21"
                     /tool="antismash"
     misc_feature    complement(13111..13120)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            13363..14841
                     /locus_tag="BP422_RS22615"
                     /old_locus_tag="BP422_22030"
     CDS             13363..14841
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018673171.1"
                     /locus_tag="BP422_RS22615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_22030"
                     /product="hypothetical protein"
                     /protein_id="WP_236841173.1"
                     /transl_table=11
                     /translation="MNQAHLLERYHQLRELAEDADAWLESEIGSDLWVDGINVFLTVSP
                     EDFLEGLERFEGNYGFKNHGIVSTIRQFHHYCREASADGEFPLYQALAVGMTWLSLQPQ
                     INGGYFNLPVQITNHSIALLLSPTYLAVWAHSYNEGIGLYLDLHSTLPTLFRPEHGRIY
                     QNAGSYLEGTHIKFPFQNYFHEIAHILFFHDVYQRVLGSEDECRSYWTHIEACIYGLEE
                     QVLAEMMEVQPDLHAIDDGFGGSKGFEDFISYRFRILGAQEDRSIPKDTVRTYIKRSMQ
                     LGEANENIPDNKVKSRILASHKLDEERLEELDTHCKLFATALQVHSLWGIKSYQRNSIP
                     AFREVVELLSPDAYCLQKLEESMRPDSWGTLEDLLSKNPFVEICSQTREKNIQVWNWRD
                     TLSRLAEMRGYLANADEPGFVETNQELFQIANRLASVIHEEKQKEGPEEQYESQYADMN
                     REISETINRLPNVEVRDHLHNLLSKPYYFVLEPN"
     misc_feature    14948..14959
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.11"
                     /tool="antismash"
     gene            15086..15442
                     /locus_tag="BP422_RS22620"
                     /old_locus_tag="BP422_22035"
     CDS             15086..15442
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016741884.1"
                     /locus_tag="BP422_RS22620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_22035"
                     /product="DUF6220 domain-containing protein"
                     /protein_id="WP_236841174.1"
                     /transl_table=11
                     /translation="MFIVLAWVFFAFIIVQVSLAGLGLFVNGEHWEMHRTLAQYFALLP
                     VGMLVLSFLGKLPVHLRWICLGLYLMIVAQFLTVIYSSNLGILSALHPVIALFLFWGSL
                     FTAKHAHNLGGKKA"
     gene            15439..16062
                     /locus_tag="BP422_RS22625"
                     /old_locus_tag="BP422_22040"
     CDS             15439..16062
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015893442.1"
                     /locus_tag="BP422_RS22625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_22040"
                     /product="DUF2306 domain-containing protein"
                     /protein_id="WP_088909606.1"
                     /transl_table=11
                     /translation="MKLPKIWWGLFLVSLLVIVPFMFPYLTLDPANSRITIIPETVQYP
                     LLVAHIVSAFIALLTGFFQFVDRIRLKKPSIHRFLGRVYVYSVFISGLLSLGSIFYAED
                     FMKATSFFVLSLAWLFTCWKGYRTAINRQYEEHRKWMIRSFGMTLVAVSARLLVPVLLL
                     LYFIFHGFSLPHGMETVIGEVLNVNIWAGLILNFVIVEWAILKK"
     gene            complement(16346..16990)
                     /locus_tag="BP422_RS22630"
                     /old_locus_tag="BP422_22045"
     CDS             complement(16346..16990)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015893443.1"
                     /locus_tag="BP422_RS22630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_22045"
                     /product="hypothetical protein"
                     /protein_id="WP_088909607.1"
                     /transl_table=11
                     /translation="MSSTLRNLLTMILLGAAGGITISFLDIEPTPMVWITFFMIAFVIL
                     IGKDLYIFKLSTNMNTVERYVKHSKQPYYRFLYAYLHDNEVEAEKELAKITSDKLRAYI
                     TIVVELHRSNYDLVEKALGKIDSIEHRHYYSAHLALLKNNQDAYNQHRSAIKNRIYQLL
                     LDAEVAYHQGNMAEAEQCGQLAIAESKGVQKYLLIKTLEKSKRSQHRNSYF"
     misc_feature    17575..17591
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 23.38"
                     /tool="antismash"
     gene            complement(17627..19612)
                     /locus_tag="BP422_RS22635"
                     /old_locus_tag="BP422_22050"
     CDS             complement(17627..19612)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF022063.4"
                     /locus_tag="BP422_RS22635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_22050"
                     /product="Z1 domain-containing protein"
                     /protein_id="WP_088909608.1"
                     /transl_table=11
                     /translation="MTLDIGARTKRFLRLLTTEINSQEAQNILSNANSLVQAVGNVDEP
                     SKQGLLFGQIQSGKTNNIMMSIALASDNGYRLFIVLTSDNTWLYDQTIRRIKSALPGLL
                     TLGKEQWNAATYDSRIQDALEHTGLVLVATKNHNILANLEQFIAQTCASELKPIIYDDE
                     ADQASLNTLINKDTNELSGVNQSIVNIRDYFPSQIYIQVTATPQALFLQCAESAFRPEF
                     TVTFQPGRDYVGGQDFYERPQQLSPMRLFNDDEVESIITHTTENVDENWHAVPTGLRQA
                     LCSFFVAASTKLLLGEGTAYSCMLHLSHLQEPHRRLETLIHGFITRLTRALLNPTSSDR
                     DLVLQYLREAYDDMRITKTDCPGFGEVCQEIIDNIVSTHTQVLISERDNKIPSYDSPFN
                     ILLGGNRLGRGVTIKGLLVTYYGRNSKNPQVDTLLQHCRMYGYRKKDMDVMRFYISRSL
                     LDVFTSVYHSDQQLWNMAQQGSPTDMQAVVLSRTNNTILRATRRNVVYLDSLAFYTPGD
                     RTFPLFPLSKNLSLLDQLLLPFDGKPQLVEVPLDILVQILNLTESEKQLVGWDDEAIRT
                     CLRNMKQMDIYRNKGYLLVRSDRDITRGSRAMLSPNDYSQFNSDYPTLTMYRYNGKVEN
                     GWEDGMPRWVPNLRFPDNRFFIISAF"
     gene            complement(19612..20568)
                     /locus_tag="BP422_RS22640"
     CDS             complement(19612..20568)
                     /EC_number="3.1.21.-"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF044296.1"
                     /locus_tag="BP422_RS22640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="restriction endonuclease PLD domain-containing
                     protein"
                     /protein_id="WP_088909609.1"
                     /transl_table=11
                     /translation="MPIYPRLYDDVINQNYALGLRRLEVLTGYASSSFVHHLIYTYENI
                     HLDVILGMAKQDNISIWDHNEYVNLCENTGRLRVRYYNGRPAIHSKIFVWRGENGDAER
                     VYTGSANLSWHGFRDYQEILVNANSDEIRAAFPPCDDLVDCTSPDVFQYFTMGFQQSPQ
                     SSSIDAGAVNHIIGDRQFVTLPLVMVNQNREVHEKSGLNWGQRHGREPNQAYIPIPSAI
                     HQQNPGFFPPRKHEFNLITDDGQSFVCVVAQDNNKALESSHDNSILGKYFRIRLGVPLG
                     GKVQTTDLTQYGRDTVRIYKIDDETYYLDFSQRGYNS"
     gene            complement(20753..21859)
                     /locus_tag="BP422_RS22645"
                     /old_locus_tag="BP422_22060"
     CDS             complement(20753..21859)
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256370.1"
                     /locus_tag="BP422_RS22645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_22060"
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_088909610.1"
                     /transl_table=11
                     /translation="MIFRKGELFNGPGGFAWGAKNARVEANGMTYAVEHTWSNDYCDSA
                     CDTYTHNICPDKPESVHRGDVRLLNLEELGEIDAFIYGFPCNDFSIVGEKKGFDGTYGP
                     LYSYGIRVLKHYQPKWFIAENVGGLQSANEGKAFLQILSEMNEAGYKLTPHLYNFADYG
                     VPQTRQRILIIGFRKDLNMEFRVPAPTHGPNRPHPYKTAYDAIMEPPILENAYNNDMPK
                     HKPKVIEFLKAIPAGQNAWYDGIPEDLRLNVKGAKMSQIYRRLEKNTPSYTVTGSGGGG
                     THMYHWEEPRALTNRERARLQTFPDEFYFHGTKEKVRQQVGMAVPPEGARIIIEAVLKT
                     YAGVEYDSIKPKWDVEQMLEQKIDSLFL"
     misc_feature    21937..21950
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 23.1"
                     /tool="antismash"
ORIGIN
        1 ttcaggatta tgcccaagtc attgaagcaa accttcaaga ggcagtagaa gatgtatcca
       61 ttgataaaca gattcgtact gtagtcgaca accaggtggc catccaattc aatgcaaaag
      121 gcaaggtaga cggtattaag gtcgcttata ttgtgacatt agtcgaaacg cctacacatt
      181 ttcatcaaat catggcttgg acagaagaaa agaggtttgc gcccctcaag caagaactgc
      241 aaaaggtcag tgcaagtttt cgcgaggtaa aatagcgaca gaacacagaa gccgcctaca
      301 tcgggtggct ttttttctta gaaaagcagg actctactcc tagttgcaga caggacaata
      361 taacgattga aaaatgtggt ttaaatgtat aattgaaggg tggtcctcct ttacatataa
      421 tgttaaagta atacaagtag tcaatttttt ctagaaaatc tattccgata agtgctaatt
      481 ttcgggtcag ggtagcgctc ttaatcctta agagttacta taaatatttc ttgagaggtg
      541 catgtacatg tttatgggga aaggaacagt ccgcgagctc ggaaatcaaa tcgacaaggt
      601 tcttggagat attaaagata tccaggcaga aatcgaccgc gactctgata aaattgacaa
      661 cgaattgaat tcatgctctc gtgagctgat caatgcacaa acaacgctgg gtgaaattca
      721 gccactgatc gaaagtctcg ttgctcaggt tggtcaaaat gcccctgatc acattaaagt
      781 gcttgttggt accattgcag atggtattac gggtaaagtg aaaaatacgt taaacaacct
      841 ggcagaggtt caaaagaacg tgaaggacgt tgacaagctg acagacgcca tcgacggaca
      901 tacggataaa attgcacaaa aagttaaaga aatcgatagc atcactgaca aagttcaaaa
      961 gtaggtgatc gaatgtcgta tataaacaat tcgaacggtt ggggtactca gcatacgaat
     1021 catgtggcaa ataaggttga ccacctcgct gacaaaattt cctttgcagt ggatatggta
     1081 gccaatgcag cgattaacct ggacggcgct caacaagagc tgcttacact ggcaaatgcg
     1141 ccctacccac cacaatctac ccaattaatt acgttagcga atcgcattgc agcgaataag
     1201 cgccaaattg atgatggctt ggctaaaatc aaagaaatgg cccgcactat cgatcaagaa
     1261 acgaatcaga tccagacccc tcctcaaaaa ggctggtaaa gaaaagagga gctcttctta
     1321 gggctcctct tcgcatttct ccgttttcat tcctctcgtg ctacttctcc atcgactctt
     1381 tatcactggt atttttgtag gatttttgat tcccccatgg aggttctgct ctcatcaagt
     1441 gccgtacaaa ataatcccat tttctacgga taaagtacgg ttccaaagaa aatccgtgat
     1501 gtcggttggg caaaatcaac atgtcaaaat ctttatccgc tttaatcaat gcattcgcca
     1561 tccgaatcgt ttgggaagga tgcacatttc catccaaatc gccactgacc aacaagagct
     1621 ttccttctag attcgctgcc agacgagcat tgtcttgatc cctgtataag tcagggtcgt
     1681 acaaaccttg atagcgttct gcccatgctg ctatatacag tcgttgatca tgattaccac
     1741 acgcggaaac cgccgtcttg taaaactcag gaaatgagag tatggccctt gtcgaagcgt
     1801 agcctccccc tgacgatccc caaatgccta cccgatcaac atccacatac gggtagcgct
     1861 ctgccaattg ctttatcgct atcacatggt caagcaaacc tcctgcttcc tccaaatttc
     1921 cgtttgaaat gtcatggaac gctttagaac gataaggtgt tcccattcca tctaggatca
     1981 atacgataaa ccccaactgc gccaatgact gtccaccacc cagcgggtca tggcttcggc
     2041 cgggatcaag cgcgaaagct ttcggtgtat tgatgatctg tggtcccccg taaatgtaat
     2101 ctacgaccgg atacttgcgg gaaggatcaa acgggaatgg gcgcagcatc accccataaa
     2161 gatcggtcac accgtctcgt gctttgacgg taatcctttc tggaaattgg taccccatct
     2221 cgagcagcat ttccacatca gcgtactcca attcgcgaat caggctgcca tctaccgagc
     2281 ggagaacagt aacaggaggc aaatcaactc gcgaaaacgt atcgacgaaa tacttcagat
     2341 taggagaaaa cacaacatca tgctctgcat cttctggcgt caacaacgac aaaccggttc
     2401 catccaggcg aacgcgatac aggtgttgat aataaggatc gcgcccttcc tcaagccccc
     2461 ctccagtgat atacacccat ccctgctgct cgtccactgc catcagccta cgtaccgtcc
     2521 aggaacccga tgtgatcgga ttgcgaatct cccccgtttt gccatcgcaa tagtagaggt
     2581 ggctccatcc atcacgctct gaatgccaga tgaacgaacc atcggccaat aatcgaaaat
     2641 tcggattgga cgctccgaga ttatgcaggt cttggtttaa aaacgtatcg ctttgttccc
     2701 gcagtagcgt gcaaacctca ccggagcaag gatccgctag ggaaaaatct acagaacggg
     2761 catcacggga catccgggcg atatagatct gtttactgtc ggatgaccat ccggccattt
     2821 gcatcccctg agtgaggggt gagactggac atgccattaa aggctcgctt tcaatccgga
     2881 ctgtggttcg cctttccaga ttacagatga ccagctctgc caatggtacg tgttgatccc
     2941 caacgagtgg ataacggtat gagtgcagta ccggtctaac tccttcccct ttcggaacag
     3001 attggatcag atccatttcg cgcacctttc tttggtcaag cctatgggtc agcagcatct
     3061 tggaatcggg tgaccaaata gctactggag gaagcttagt cccattcaat cgctccgcca
     3121 tgatcgagag cctacattca ggttgggttc cgtaatcata gtaagccttg ccgtcatagg
     3181 taagcctgat acgccttccg gtttcgagtg atcgaacata caagttgtga tcatatacga
     3241 acgccaccca ttttccatct ggagacggca gttcatccat cgacggcttt ttaaaatttt
     3301 ttaagggctt gcaggtgtat tcctgcaaat cacagctcca aacagtaccg cccacctcaa
     3361 actggaatgc cgatccactc tcggtaaaca taagttcccc aagtggcaat gtttctgcat
     3421 catacccttt gcctgcacta cctgacaaac agacagctaa tcgttcgtga tcgaatgctg
     3481 gctgactcgt attctgttca gcgtccacga tcacatattg cctcccacta cccttttcca
     3541 gacgtatttc acggatgtac cagaactgac tcccgctaat ccaccgcggc ctgacaaaag
     3601 cattgaatac tgcgtcgtaa taattggcgg gatgcaagcg ttgtgccctc tcatactgtt
     3661 cccatgtgac aacattctcc atgtcaatcc ccctctttca aagtgagata caagaatcat
     3721 gtgcttcaca tttttgaaaa acaaaatccc ttatttatcc aattattaca tgcatatgta
     3781 aaaagcggga taatatatct acgaatttac ttttttagca cctgacacgt gaattttttt
     3841 caagaagaaa gaggcgatct ctcgcctctt cgctcttaac atagattact ccgcttccgc
     3901 tatcatggtt tggttcctac ccgatctctt cgccttgtac atggcctcgt cagctaattg
     3961 cagcaggcct tcaggagaaa cagcatgaga aggatagtgc gcaatgcctt gtgacacagt
     4021 tatcgactgc ccgattgggt ttgtacgtgt ttctaaagca tgacgtattc gttcggccac
     4081 ctgatatgcc tcctccaaac cagcatgaga aacgaggaca acaaattcct caccgccaaa
     4141 gcgacagctg acatcatcag gccgaagtaa ggaagaaatg attttcgcaa aatgcctcaa
     4201 cacttcatct ccagccggat ggccatatgt gtcattaata gctttaaatc gatcaaggtc
     4261 catcataata gtagaaaagg ggatttgctc cgccatccat tcttgcatgg tttcctcaag
     4321 cgttcttcgg ttcgttagcc ctgtcaaagg gtctgttcgt gcatcatgag ccaattgctc
     4381 cgtctgcttt ttgatattgg ttaaggcaaa gcgaatggca tgagataaca gctcggcctc
     4441 ccgattccaa tgctgaccac cctcagagag ttccacctct tctctcccgg ccttactcac
     4501 aagattggcc aaagatacga aaggtttcgc tactttgcgc gcaagcccga tcacaactag
     4561 cattaataat aaaaagggag gcagcatata caacaaaatg gattgaatat cacgatttaa
     4621 ttgttcttgc acaacactga taggtgacac gacaacaaca ccccagtcat tcgctggaac
     4681 ctttacgtac gcggctaata ccgtctgtcc cctcaagttg acgatctgtt catagccact
     4741 tttcccctgg atcagcttgc gtacggctgg attggcactg atatcctctc caatccggtt
     4801 tgtatcacgg tggaacaaca aatgaccatc agatcctacg atgtaaaaag aggaacctag
     4861 ttcgtctgtt ggattacttc caaagatcgt attcaaaatg ttatcttcat gaagataaat
     4921 cgttcccccg ataatccccc gatagaaccc gtccttatca aatatgggtt cgctcatcaa
     4981 tacaatgagt cgcttcgtgc ttgctgttac gtaaggctcg gaaatgtatg gcttttttaa
     5041 tgccaatgcc gttttccccg ttttcgtttt aacatagctt cctactgtac caatagatcc
     5101 gggagacaca cctcgaacaa gcccggtctc atctaccacg gaaaccgaat taaaatagtt
     5161 gtttgtacga cggatcaact caagcttgga atatacctca tccgcattca tttctttccg
     5221 attggaaaag atgctcgccg aatactgtaa gctactgcgc atggatttaa tcaaagcgtc
     5281 tagagtatga ctcattttga ttgcactcgt gtgatttaag gtcaaagtcg tgttaatcag
     5341 tgcctgtttt ttggattgat agaatgagag gagtaggatg gtgacagtca agacaacgga
     5401 taaagagact agtcctgtta aaagtgtcac taggctaatc tttttcgggt ggtttgctct
     5461 tttttgtaag acaggcattg gatgccccct tgtatttagc atgctagcta ttaagcaata
     5521 aataaaaaat agtctaacac tccataatat tcatttcaag tatacaagga agttcaagca
     5581 tccgaccttg gctttgtgac ataaaaaagc cacctttgtc aggtggtagt ttcggcaatc
     5641 atcatctagg agacccgaaa gctttgtctt ctcatccaca tcgtggcaac ccacttttct
     5701 ccatggatca cttgtgtccc tgcatgcaaa gtgagctcgt ttaattcttg atttctataa
     5761 aaatattcaa agtagacagc catgcctttg gtagggaaaa cagataaatg aagcaaaggg
     5821 aacaccgttt caccgccttg ctccacatca ttcaaataca tcacgagcgt actaatgcga
     5881 ttgttcgtac ttgctcgact cgtttctgca aagaaatcat aatgaggttt atattcttga
     5941 ccaggggtat atcgtagaac ttgcaagcca tcaccatgct caatcggaat gttcatgatt
     6001 tgagagattc gcttctcgat ccttgtaatc gtctccatct gctcgcagaa catcccgctg
     6061 ctcgttcgaa tagagtggac tgaacgatct tcgcctattt ttgagcgttg caatcgttct
     6121 ctggaatgtt cgattagctc ttcgcattcg ctatcgctaa gcacatttcc taacacgaca
     6181 accagcggtt cctcatactt ggcaagaatt tgaatctctc gatcttctgt cttgatccta
     6241 tttccagtat gatcaaatat tgtccgttct ttctctgtcg tgtgccctat catcgttatg
     6301 caccccatat atatgacatc cgtcgcaaaa aatagaatac cataaaatgg gtaacttcaa
     6361 tcagtaaact ttagaaaaat tagaatattt ttaactgtta cttgaggtct ttggcttaag
     6421 aacaaaaccc gtctcctata tatcaaggga cgggtttgta aagcatcttc taagtggcat
     6481 gtgtctatat gaaaagttat tttcatgcct ccttttgttt ttgaacgtgg tacatattct
     6541 tccggaatac actgatcatg tatccgcata tcccacctat tagagaaggc agcagccagc
     6601 ccaaacctac gtcatacagt gggaggtact gagtaaaata atgatggaca acactgtttt
     6661 caactccagc agcctttaat ccatcaaaca gactaatcag aaaagtgaaa agtaaacttc
     6721 cttgatacac ttctggtctg ccgttgaagg ctttgtgcag aaacgtcaag aagagtaacg
     6781 agatcgcaat cgggtacatg gtcattaaga tcggaacaga gaccttaatg agctgcgtta
     6841 atccaatatt agcaacgagt gtactgaaaa cagacaaaat aaccgcaaat tttttgtaag
     6901 agatcttcgg aaacaatgaa tgaaaaaagg aagagcaggc tgtaataagc cctacacttg
     6961 tagtcaaaca tgccactgta atcattaatc ccaataaaat ggcaccatac gaaccaaagt
     7021 agtaattcga aactttcgcc aaaacctcag cgccattttc taagtggccc aacttttcta
     7081 cactagaagc acccataaag gaaagggccg tatagaaaag tgcgaggagt aaacccgcta
     7141 tcattgttgc ttttgcgcac gtagccaaaa cttgttttct ggcagtaact cctttttctt
     7201 taatcgcatt cacgatgatc attccaaaaa caaaagcgac aagcgcatcc aatgttaaat
     7261 acccttcttg aaaacccttg aaaaatacat gggatgtata atctgcttgc ggagcttgaa
     7321 gggctccgat tggataaaca atcgctgtta ctgcgataat tccgataaaa gttaatttaa
     7381 taggagttaa gatttttccg actacatgga taattttcat aggattgagc gataagaagc
     7441 aggcaattgc gaaaaacagg atggtaaata gaatcagcgg tgccgaacct gcctgttctg
     7501 ataggaaagg ctttacccca atatcaaacg atacattgcc ggatctaggt atcgcaaaaa
     7561 acggaccgat tgctagatag agaacaatcg tgaataccat tccgaatacg ggatgcacgc
     7621 gactggctaa ggagcgcaag tcgtcttttc ccgaataaac aaaggctgtg attgctagca
     7681 aaggcaaccc aacccccgta accaaaaaac ctgcattggc tatccataca tttgttcctg
     7741 ctaattggcc aagcattggc gggaaaatta aattacccgc tccaaaaaac agagcaaata
     7801 acatgaatcc tatcgtaact acaaaagaaa ctggcgcttt ctgtgacaca acaaacctcc
     7861 taattattct atatctctca ttcataaatg ttcatgctta ccaatcctaa atttattcat
     7921 tctgatacta tactctaaat aaacattctg ttcaaacaca tataactcag agtacgcccg
     7981 caagagaaag aattatcttt ctcttccaaa tatggagtgg gctaggtact ccatggttgc
     8041 cggaaatcaa gcttacatcc ctgatacagt atactacaag catccttagt gtcgcatctg
     8101 gaagtacctt ccgatgttaa cttacatatt tcggtgtgga tctgcctggg tgaaaaataa
     8161 taaagggaac aaatctagtt acgatcgttc ccccaaaacc tcttcattct tcctttcatt
     8221 ccgttttatt tgaaactcct tgtaaatgca tgaggtaccc tagtttgaat ggggattact
     8281 attttaggct ctagcagatt tcttttgaac tctgtaacca tgtcccttta gcacgaatag
     8341 aatgtacaag ggttcgaggc cattgcgcct gcattaggaa cctaaagggg gggattttta
     8401 aatggccttc aaatctcgta atgtcatggc gcgtgtgtta ggtcagggcg gcgatggtgg
     8461 tcaagttttc ccaaactgtc tgatcacact tgcggtacct acgcctgctc agaggagatc
     8521 cggctgtgtc gatgcccaca gtctttgtca agtagttagc gtctgtaacg tcgtcaccgt
     8581 ctgtaatctc acccttaacc cacaggtgct cgaggacgct ggtattgaat tccttgatcc
     8641 acccaaagct caagtgagta aagaggtgcg tgaactgaag cgacaaatcg agcgcatctt
     8701 tgaggaattt gaggaaagag actaataatc gtggtcagtt ccctgcttaa ccggtagggg
     8761 ctgccctaca taccggagcg cattcgggcg ccgtactcat actcataatg gaggggaacc
     8821 ggacatgtca tttcatttgc aacaggccac gttgcgggcc aaccccgctt acaaactggt
     8881 cctctatgat cggctgcctg ccgaagaaca gaaagcgttg gatgagctgc ggcatgatcc
     8941 tgacttctat gggtttcttg tgcccattga gggaacattg gccatgaaag cagtgtgccg
     9001 ggagacggcc ctcttgttcc tgacgcttca aaatcccggt tccgtaccga cctatgtcca
     9061 gaagttatat ggaggcgcat gggaagatga tctcttcgag ctgatcctag acgatgtaat
     9121 agaggtggag gttggaggcc aatttcattc aggggctgcc gctcaggcgc tttgcggtaa
     9181 atccgcgccc ataagcaagg ggcatattgg acgtctgtca atggcggctc tccagtacgg
     9241 tgaagccttg ggtatcacaa aggttcctgt cctctcccgg cgattgtatg acttcaaccg
     9301 tcttccagcc acatccgaat ggcaccgcat gataccagac cattcggcgt tgctggccta
     9361 tgcaggcatc caacccgacg gaccggttca atcgaaacta caacgccact ggatagccgt
     9421 ggcgcattca cgcgccaatg ggtggctgac atggacgaac cagaacccag ccacggccat
     9481 acggcccgac agcatgatct acaagctata cgtaagcccc cacccatcct gcttggcgga
     9541 tgcattagca gcggccattg cgacctttac tgagtgtgcg gtgcccaact ttaagctcgg
     9601 cagcgatctg tacggaatct tgcggcccga taagctcatt gcgtatttct ccagtctgga
     9661 agaagtgatg gcggtggctg acaaactgaa actgcggttg acagggtgcc cggctcaggg
     9721 tgtgcccttt acgggagaac tggagagcag tggacttctt tcatggggaa tggatccccc
     9781 gcaagtgccg tccatcgcgt ggtcgccgat ggaaagttgg cgactatgga tcacgaacca
     9841 tctcgcatcc gccctgatcg cagcccgact ggcacctgca accgggctct ccccttggga
     9901 gtttgctatg gccagaatcc gcttggctaa cgtggacccg gagacctgga ccccattacc
     9961 atcgctgagg tggaatctac cagacgagga gggatagcag atgcgcatca cagacgtttt
    10021 cgtattaccc gatgacgtgc aaataaaggc agtatctgag ttacccgcgg atctcttggg
    10081 ggatctggtt tgtgaagaag gcgagcaggc aatcattcgg ttaggaacgc ggatgcccat
    10141 ccgcctgctc gatgaagagg gagtgaaact tctgctccat tttcgcccgg cgaagactat
    10201 tggagcggcc attattgaat actgccgtgg aaacggaggc gatccccagt ccatcctgga
    10261 agaagcttac ccaatgctgc ggctcctgat cgatagcaat ctgcttgtgc cagccgactc
    10321 ccccgccgcc cggccagtta ctcccagttt tcgtccaagg gagagcgttg caggctgtag
    10381 agtggttcgc tgccttcaat ggctaaccga cagcgaacta taccacgtta aagatagtaa
    10441 cggagtaaac gcagcattaa agattgcccg acctgaattt gccgaaatga tgaggccctt
    10501 atttacccat gaggcggagg ttctaaggtg tctagacggt cgtgtaaacc cgccattact
    10561 cgcggccggg gacctgaacg gtcgtcctta tctccgcatc ggctggtgtc ctggtgaaca
    10621 cgtggacaca agagcgcaca acctccgccc cctgcaaacc accctcggcc gacagcgttt
    10681 gctgaagctt agctgtgcgg tgcttgatgc ctacacgcat ctgcatgcgc aaggcgtagt
    10741 gcatggtgat gtccatcccc gcaatatcct tattagtgat gatggacagg tttctatcat
    10801 cgactttggc ttctcccggc gagagatcca taaagattct ggcgggatgc caaatcgtgg
    10861 aggcattccc tactattatg aaccggagta cgcttgggcc agcctcgagc ggcgttctcc
    10921 tccccatgca acacaagccg gagagcagta tgcactagcg gcactgctgt atgaactgat
    10981 cacaggtaag ccctacacca atttctcggt cgaaatagag agcatgctgc aacagatcac
    11041 aaaagagcct cccctgccct ttgcggacca gggggttgcc ccatggcctg agatcgaagc
    11101 aattctttgt cgggcactta gcaaatctcc accggaccgg tatgggtcct tggccgagtt
    11161 ttccagccat atgcggacac tcttgcactg tgaggcagtc gaccactcgc cgccagcaat
    11221 cccggaaacg gcagcccgca tcgtttttga agtgctggat cgagtgggcc caggtggatc
    11281 cctttccttc actgacttga ctccacctac ctgctctgtt tactatggcg cagcgggtat
    11341 tgccttcgca ctctatcgca tggcttgttc ccgaggagat gcgctgctgc tgagtcaggc
    11401 cgatgtctgg acgtgttggg ccgagagtgc gatcactggc accgatgcct tccgcatccc
    11461 tgaagcccaa ctaccgcccg agtggttctg tacggtcact ccgtttcacc aggcttcagg
    11521 ggtttaccta atccgagggc tggtcagcca agctttgggc gatccggcaa gcatgcagag
    11581 ggcgatagac tccttcgtaa cggcttgcag tacgccatgc accaatccgg acctgactct
    11641 tggcaacgcc ggtgtgctgc tgagctgtgg catgttgctg gaagctgcac cggacatccc
    11701 gggcatggat atcacgaatt tgttggcgct gggtaatcag ctggtggaat ccttgtgggc
    11761 ctccagtatc actgtgccaa atagttacct gggtatcgcc cacgggtggg ccggcattct
    11821 ttacgctact cttcattggc acaggatcac taagaatccc cttcccgaag gcactttgaa
    11881 ggccttggaa tggcttgcgg cacaggccaa agaaaatgga aacagggctt ggtggccggt
    11941 tacgactgac agtgaacaag cggcacaggg atggtgtcat ggcagtgttg gctacgtatg
    12001 gctttggacc attgcgcatc atttgtttaa gcaagaatca tacttgcaat tggcgataca
    12061 ggcagcccaa tatgcgtggg acactccggc tgtaaacggt acgatctgct gtgggatggc
    12121 tggtcaggcg tacgcaatgc tccacttata tcgctacacg ggtgagaaac attggttaca
    12181 gaaggcattc gccctcaccg agggggccgc ggacagcatt cagcgggtcg atgagagacg
    12241 tgatagtctt tttaagggtc atttgggtgt tgccgtatta gcagtggact tggaacatcc
    12301 gaaccgtgct tgttttcctc tgttcgaaat tgagggttgg ccaggatcgc agaaggtata
    12361 gacgatttta ggtcatgtcg gcaagaggcg ttgctaggcc tctctcattc tccagtagca
    12421 gcagcgtgga accgaagcaa ctgtgagcat gacgcagaac ctcctgcagg tgtggagcct
    12481 cctagctgaa atggcggcgg acccgtcgta cagatattac aaagctgggc tcgtcacctc
    12541 gttttcgata gtctttaatc tttttaaccg caacaagcta tttaatggga aagtcgtaac
    12601 ctatctgcgt tcgtcatcta aacagcaatc tgaatgcgag ctatccatca ctgcacaccg
    12661 cgaagccata ctcgtccaca aattcaagtg ttttaactga agtcttgagg atcgtatcat
    12721 ttacaaagcc ctgctcaata agcagggtat tttttgtact ctgttgttga tcactctgct
    12781 agacagtaat tcgtaaatca ttgtgctaac cgactcgaat agaacacgag gttcctcgcc
    12841 ttggtacttt tttatacgta atgctttgtg aaatgtttat atatttcaga aaatatttat
    12901 ttttcttaac ttgaaatcaa ttgacaattt tccttataat tacaattaag gtttttgttg
    12961 ataaaaaatt atatatagtt ccatccaatt ggaaaggagg tgtaaactca tgaaaaaagt
    13021 cgtaaaagta gaagtaaaca agaacaaaca agtgcaagaa atgaaagcgt tgaaatggcg
    13081 cgtagttgtt gatgtttgct tgtaagagag tcaaaaaaca tgcatgattt ccgtaaaatg
    13141 ataaaagtta ggaaggacgg gattgcccat gaaaaaggta gtgaaagcac aactttctaa
    13201 caatcaaaac gttcaagaaa tgaaattgct tctccatcca attttaacac ttgtgtgtaa
    13261 aatgtaattg attgttcaag agcacctcgt gtgctcttga attttttgca taaagttctc
    13321 gctttcgagg cagtaagcaa acatgacagg atggtgcagt ttatgaatca agcgcatttg
    13381 ttggaaagat atcatcagtt gagagagctt gccgaggacg cagacgcatg gctagaatct
    13441 gagatcggtt ccgatctttg ggtggatgga attaacgtat ttttaacagt atcaccagag
    13501 gattttcttg agggacttga gcgattcgaa ggtaactacg gttttaagaa ccatggaatt
    13561 gtaagcacta tacgtcaatt tcatcattat tgtagagaag catcagcgga tggtgaattc
    13621 ccattatacc aggctctagc agttgggatg acctggttaa gcttacagcc tcaaataaac
    13681 ggcggctatt tcaacttgcc tgttcaaatt accaatcact ccatcgcttt gttgttaagc
    13741 ccaacctatt tagctgtttg ggcacatagt tacaatgagg ggattggatt atacttagat
    13801 ttacactcga cactacccac gttattccga ccagagcatg gacggatcta tcaaaatgca
    13861 gggtcgtatt tggagggaac tcacatcaag tttccttttc aaaattactt ccatgaaatt
    13921 gctcatatcc tattcttcca tgacgtctat caacgtgtgc ttgggagcga agacgaatgt
    13981 agaagctact ggacacatat cgaggcatgc atttacggac ttgaggagca ggtgctcgct
    14041 gaaatgatgg aagtccaacc agatttgcat gccatcgatg atggatttgg aggtagtaaa
    14101 ggattcgagg attttatctc ttatcgcttt aggattcttg gtgctcaaga ggatagatcg
    14161 ataccaaaag acacggttcg cacttacatc aaaagaagca tgcaattagg cgaagcgaat
    14221 gaaaacatcc ctgataataa ggtaaaatcg agaatccttg ctagccataa gctagatgag
    14281 gaacgattag aagagcttga tactcactgc aagttatttg ctacggcatt gcaggtacat
    14341 agcttgtggg gaatcaaaag ctatcaacga aactcgattc cagcgtttcg tgaagtggta
    14401 gagttactct cccctgatgc gtactgtctg caaaaactcg aagaatctat gcgaccagat
    14461 tcatggggta cattagagga tttgttatcg aaaaatccgt ttgttgagat atgctctcaa
    14521 acaagagaaa aaaatattca agtatggaac tggcgtgata ccctgtctcg gttggcagaa
    14581 atgcgcggat atttggcaaa cgctgatgaa cctggtttcg ttgagaccaa tcaagagcta
    14641 tttcagatag caaatcgttt agctagcgta attcacgagg agaaacaaaa agagggcccg
    14701 gaagagcagt atgagagcca gtacgccgac atgaaccgag aaattagcga aacaataaac
    14761 cgattgccaa acgtggaagt acgcgatcat ttgcacaatc ttctctccaa accctattac
    14821 tttgtgttag agcccaatta ataactgatt atgagtcaaa gaccggcgat acttcctatg
    14881 ttggtctttg ttttttatgc aatttataca taagccgatt tgacattcag attttatcct
    14941 ttttttagaa aacgtttagg gtttgttttg aacttgcgtt taggatgggg gtaagacaaa
    15001 tgattcagat gggagcggtt tttgcatgct tgagataaat gagaagattc cccttccccc
    15061 tcctcgtgtg aaaatatgga ggtacatgtt tattgtcctc gcatgggtgt tttttgcttt
    15121 cattattgtt caggtatcac tggcaggtct tgggctgttc gtcaacggag aacattggga
    15181 aatgcataga accttggctc aatattttgc attgcttcca gtcggcatgc tggttctctc
    15241 tttccttgga aagctgcccg tacatcttcg ttggatttgt ctcggattat acctcatgat
    15301 cgttgcgcag ttccttaccg ttatttattc ctctaactta ggaatcctct ctgcgcttca
    15361 cccggtcatt gccttgtttc tcttctgggg atctctcttc accgcaaaac atgcacataa
    15421 tttagggggg aagaaggcat gaaactcccc aaaatttggt gggggttatt tcttgtatct
    15481 ctactggtca tcgttccatt tatgtttccc tatttaacat tagaccctgc aaacagtcga
    15541 ataacgatta ttcctgagac tgttcagtat cctttattag ttgctcatat cgtttctgct
    15601 ttcattgctc ttctcactgg ctttttccag tttgtcgacc gaattcgcct gaagaaacca
    15661 agcatacatc gttttcttgg gagagtttat gtgtacagtg tgtttatcag cggactcctc
    15721 tctttaggga gtatctttta tgcagaagat ttcatgaagg caacaagctt ctttgtctta
    15781 tctttggctt ggctgtttac ttgctggaaa gggtatcgta ccgcaatcaa caggcaatat
    15841 gaggaacacc gaaaatggat gattcgcagt tttggaatga cactagttgc cgtaagcgcg
    15901 cgtcttttgg tgccagtatt actccttctg tactttattt ttcacggttt ttctcttccg
    15961 catggtatgg aaaccgtcat tggagaggta ttaaatgtaa atatttgggc tgggcttatt
    16021 cttaattttg ttattgtcga gtgggctata ttgaagaaat aggcttcgtc acgccacgtg
    16081 ggcgatggaa tggtagcgca aagaaggact gtctcgatcg ttgaatcggg acagtccttc
    16141 ttgtcgaaga aaaacgcaga tctagatatg ttttaccggc acacaaaaat caacgatgtg
    16201 cctctctcct acttgctcac ctgtataata ttcgtagcat tgacgatcat ctggtacgta
    16261 tccactctgt ggtagccatg tgacgacgcg gttgattcgt gagacacggg cctcccggaa
    16321 tcagaaaacg aactaggaaa gtgacctaga agtaagagtt ccgatgctga ctgcgtttgc
    16381 ttttttcaag tgtcttgatc agcaaatatt tttgtacgcc cttggattcg gcaatggcta
    16441 gttgaccgca ttgttctgct tctgccatgt ttccttggtg ataggccact tcagcatcga
    16501 gtaacaactg gtagatacgg tttttgatcg cggagcggtg ttggttgtag gcatcttggt
    16561 tgttttttag tagtgctaaa tgtgcggagt aataatgtcg atgctcgatt gaatcaatct
    16621 tgccgagtgc tttttctacc agatcgtagt tgctgcgatg gagttctacc actatcgtga
    16681 tgtaggcgcg gagtttatcg gatgtaattt tggctagttc tttctcggct tccacctcgt
    16741 tgtcgtgtag ataggcgtat aagaaacgat agtaaggttg ttttgaatgt ttgacgtagc
    16801 gttcaactgt gttcatgttg gtgctgagtt taaaaatgta caggtctttg ccgatgagaa
    16861 tgacgaaagc gatcatgaaa aaagtgatcc ataccatagg cgtaggttct atgtctagga
    16921 aggagatcgt tatgccacct gcggctccga ggaggatcat ggttagtagg ttacgtaggg
    16981 tggaagacat gatggtgctc ctttgcttgg aagtattcct ttagtttagc ataagggaaa
    17041 gactcttcat ccaaaaattg gggaaagggt cgtttgtagg ttttgggacg ctcctcaaaa
    17101 ctgcccaaag tttctcacat cagtgcgccc agcgactcga atagcattag gtttagtcca
    17161 gtatacatgg gctgaatctc taaaacgctt gttatattgt tcttcccaaa ccgcaatgta
    17221 tagaatatag ttattatcca ctttaattct tttttaattt ttctcctgta atggtcaata
    17281 tctttagtgt ttgaaagtta gttatccatt gttaagttgc ttctcttcct cgatttagta
    17341 atcaattgta ctgctacgtt tattcgtttc ctgcctcttg agcacggtct aactatcgac
    17401 taggtatcat aggaataata tatattaaaa acacgagact actaaaaaag tcgtgtcctt
    17461 tttatcaagg acacgacttt ttttacaatc tactttgggt aaatcctaag tataaataac
    17521 tgatattttg gatatttgct aaaagatgct cgtatcccct aacgagtggg gcatttgaac
    17581 aagactagca agttccatat gcttttatta gctaactagt taagatttag aatgccgata
    17641 ttatgaaaaa tcgattatcg gggaatctaa gatttggtac ccatcgaggc attccatctt
    17701 cccatccatt ttcaactttt ccgttatacc tatacatagt aagggttggg taatcggaat
    17761 taaactgact ataatcattc ggagagagca ttgctcgact ccccctagta atgtcacgat
    17821 cacttcttac cagtaaatat cctttgtttc gatagatatc catttgcttc atatttctta
    17881 agcaagtacg gatggcttca tcatcccagc caacaagctg tttttcagat tccgttaaat
    17941 taagtatttg cactagtata tctagtggaa cttcaacaag ctgcggcttt ccatcaaaag
    18001 ggagcagaag ctgatccaag agtgataggt ttttagatag cggaaataaa gggaaagttc
    18061 gatcgccagg cgtataaaaa gccagagaat ctaagtagac aacatttcgt ctagttgctc
    18121 ttaatattgt gttattcgtt ctggaaagaa ccacggcctg catatccgtt ggtgagccct
    18181 gctgtgccat attccataac tgttggtcgg agtggtaaac actagtaaac acatccagca
    18241 aagatcgaga tatgtaaaaa cgcattacgt ccatatcttt ttttcgataa ccatacatac
    18301 gacaatgttg aagcaaagtg tccacctgtg gatttttgct gttccgcccg taatatgtta
    18361 ccagcagacc tttaatcgtt acacctctcc ctaaccgatt cccccctagt aggatgttaa
    18421 atggagaatc ataactaggt attttattgt ctctctcaga aattaacacc tgggtatgtg
    18481 tacttacaat attatcaata atttcctgac aaacttcacc aaaacccgga caatcggttt
    18541 tcgttatcct catatcatcg taagcttctc tgagatattg taagacgaga tcgcgatcag
    18601 atgatgtcgg attcaataag gcacgagtaa gtcttgtaat aaatccgtgg attaatgttt
    18661 ccagtctcct atgtggctct tgaagatgac ttagatgcaa catgcaggaa tatgcagttc
    18721 cttcacccaa caggagtttc gtagatgctg ctacaaagaa agaacacagt gcttgcctga
    18781 gaccagtagg tacagcatgc cagttttcat cgacgttttc ggtcgtatgg gtaatgatgc
    18841 tttctacctc gtcatcgtta aacagtctca ttggacttaa ttgctgagga cgttcataaa
    18901 aatcctgtcc tcctacgtaa tctctgcctg gctgaaaggt taccgtgaat tctggacgaa
    18961 aggcactttc tgcgcattgt aaaaataatg cttgtggggt agctgtcacc tgtatataaa
    19021 tttgggaagg gaaataatcc ctgatattta caatgctttg gttaactccg cttaactcgt
    19081 tcgtatcctt atttataagt gtattcaaac tagcttgatc agcctcatcg tcgtagataa
    19141 tcggcttcaa ttcagatgca catgtctgag ctataaactg ctctaaattt gccaaaatgt
    19201 tatggttttt tgtagcaacc agaacaagcc cggtatgctc caatgcgtcc tgaattcggg
    19261 aatcatatgt ggctgcgttc cattgctcct ttccaagtgt gagaagtcct ggtaaagctg
    19321 atttaattct tcttattgtc tgatcataga gccaagtgtt atcagatgta agtactataa
    19381 atagtctgta accgttatcg gaagccaatg caattgacat cataatgtta tttgttttac
    19441 cactttgaat ttgcccaaat agcaatcctt gcttgctcgg ttcatcgaca tttccgacag
    19501 cttgaaccaa actgtttgca ttactcaata tattttgagc ttcttgtgaa tttatctccg
    19561 tggtgagaag ccttagaaac ctcttagtac gtgcccctat atccaatgtc attacgaatt
    19621 ataccccctt tgactaaagt caagataata agtctcgtca tcgattttgt atatgcgaac
    19681 ggtatcacgc ccatattgag tcaaatcggt ggtttgaact ttacctccta gtggtacacc
    19741 aagacgaatt ctgaagtatt ttccaaggat gctgttgtca tggctggact ctagggcttt
    19801 attgttgtct tgtgcgacaa cacagacaaa actctgaccg tcatctgtaa tcagattaaa
    19861 ctcgtgctta cgtggaggaa aaaaaccggg attttgttga tgaatagcgc taggaattgg
    19921 aatgtatgct tgattgggtt cccttccgtg tcgctggccc caattcaatc cgcttttttc
    19981 atgaacttct ctgttctggt tcaccatgac caatggtagg gtaacgaact gccggtcccc
    20041 aatgatatga ttaacagcac cggcatctat gctactagat tgtggtgatt gctgaaatcc
    20101 catggtaaaa tactgaaaca catcgggtga ggtacagtct actagatcgt cacatggagg
    20161 aaaagctgct cttatctcat cggaatttgc attgacaaga atctcctggt agtcccgaaa
    20221 tccatgccag gataaatttg ctgaacctgt gtaaactctt tcggcatctc cgttctctcc
    20281 acgccagaca aaaatcttag aatgaattgc cggtcttccg ttgtaataac gtacacggag
    20341 tctcccggta ttttcgcaaa ggttcacgta ctcattatga tcccatatcg aaatattatc
    20401 ttgcttagcc atgccaagga ttacatcgag atgaatattt tcataggtgt agattaagtg
    20461 gtgtacaaaa ctggaagaag catatccagt cagtacttcc aatcttcgca acccgagggc
    20521 ataattttga ttgattacat catcataaag tcgtgggtaa atgggcataa acgacactcc
    20581 attgcgaaat ttcgaaaaat aagttcactt atattatatt accataacct tgaggttcgg
    20641 taaccctaaa tcttctttgt tacaaaagta tataggaatg tttacctata tttctaatta
    20701 tgcttattaa gaaaaaaaaa atcccttttt tgaaagggac gtcaagaaat tattatagaa
    20761 aaagtgagtc tattttttgc tctaacatct gctctacatc ccattttggt ttaatggaat
    20821 catattctac tccggcgtag gttttcaaaa cggcttcaat gatgatacga gcaccctccg
    20881 gcggaactgc cataccaact tgttggcgca ctttttcctt tgtcccatga aaataaaatt
    20941 cgtctggaaa ggtttgtagt cgtgctcgct ctcgatttgt caacgccctc ggttcctccc
    21001 agtgatacat atgtgtacca cctccaccac tcccagttac agtataagat ggagtgtttt
    21061 tctccaagcg tcggtaaatc tggctcattt ttgcaccttt gacattgagg cgtaagtctt
    21121 caggtatgcc atcgtaccaa gcattttgtc ctgcaggaat agctttcaaa aattcaatga
    21181 cttttggttt atgctttggc atatcgttgt tatatgcatt ctctaatatc ggtggctcca
    21241 ttattgcgtc gtatgcggtt ttgtaaggat gtggtctatt agggccgtga gttggagctg
    21301 gcaccctaaa ctccatgttt aaatctttgc gaaaaccgat aatcaagatt ctttgccgtg
    21361 tctgaggcac accataatct gcaaagttgt atagatgcgg agttagcttg taaccagctt
    21421 cattcatttc cgatagaatt tgaagaaatg ccttaccttc attcgcactc tgtagcccac
    21481 caacattttc agcaataaac cacttaggtt gatagtgttt tagtacacgt attccataac
    21541 tataaagcgg accataggta ccatcaaatc cctttttctc tcctacgatg ctaaaatcat
    21601 tgcatggaaa gccatagata aaagcatcaa tttcacctaa ttcctccaag ttaagtagcc
    21661 ttacatcccc acgatgaacc gattccggtt tatccgggca aatattgtgg gtatacgtat
    21721 cacatgcact gtcgcagtaa tcgttcgacc aagtatgttc gacggcatag gtcataccat
    21781 ttgcttctac ccgagcattt tttgcgcccc aggcgaatcc tccgggtcca ttaaataatt
    21841 caccttttcg aaagatcatt tttgcctccc cttgccgtac taactaatat attttactat
    21901 tataccactt cttttatcat ttatcgacgc ccaaaaggaa catacgttct caattaaaga
    21961 cagaaactta taccatacta gtagaggagt tctgaggttt ctcgttatat attgctattg
    22021 tttgtaatat tatagcgcaa tatttctaca tagtagaatg taaatattct ggcttgattt
    22081 gtattagcca tgttgtactt cccagataga aatgcaactc caaacctttc tggtttttga
    22141 aaggagcctc cttcctcttt ttcaagtaat ttcgagaata tccggctcct tgtctttctc
    22201 caaaaaaacc tttcaattca caaaagccaa gcttggaaat aaaacttgta attaacttga
    22261 ttcaactata aataaaaata gacaacagat tatctgatgt ctattatttt gtgatttaaa
    22321 cgataatttt gccgctaatt taatcaaatt tttgccagaa a
//
