LOCUS       NZ_CP018145            11935 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Brevibacillus formosus strain NF2 chromosome, complete genome.
ACCESSION   NZ_CP018145
VERSION     NZ_CP018145.1
KEYWORDS    RefSeq.
SOURCE      Brevibacillus formosus
  ORGANISM  Brevibacillus formosus
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Brevibacillus.
REFERENCE   1  (bases 1 to 6437015)
  AUTHORS   Fu,B. and Li,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2016) Insitute of Enzyme Engineering, Shaanxi
            Academy of Sciences, No 16, East Renmin Road, Xi'an, Shaanxi 710600,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Coverage                   :: 167.68
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/05/2024 02:46:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,168
            CDSs (total)                      :: 5,986
            Genes (coding)                    :: 5,821
            CDSs (with protein)               :: 5,821
            Genes (RNA)                       :: 182
            rRNAs                             :: 16, 17, 17 (5S, 16S, 23S)
            complete rRNAs                    :: 16, 17, 17 (5S, 16S, 23S)
            tRNAs                             :: 127
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 85 of 165
            Pseudo Genes (incomplete)         :: 95 of 165
            Pseudo Genes (internal stop)      :: 22 of 165
            Pseudo Genes (multiple problems)  :: 35 of 165
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:35:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1802247
            Orig. end                         :: 1814182
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP018145.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11935
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11935
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11935
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6935](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6935
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    402..415
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.62"
                     /tool="antismash"
     misc_feature    888..902
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 21.26"
                     /tool="antismash"
     misc_feature    complement(1545..1554)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 17.8"
                     /tool="antismash"
     misc_feature    complement(1830..1843)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.15"
                     /tool="antismash"
     misc_feature    2091..2104
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 21.2"
                     /tool="antismash"
     gene            2745..4403
                     /locus_tag="BP422_RS09020"
                     /old_locus_tag="BP422_08780"
     CDS             2745..4403
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718009.1"
                     /locus_tag="BP422_RS09020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_08780"
                     /product="hypothetical protein"
                     /protein_id="WP_088907440.1"
                     /transl_table=11
                     /translation="MADFTSEFHRFIALCNEEYLIKYANKGIYNRSIKEMEKGMTVSYE
                     FGDDYVECALSDDSVCRLHADMNRFSCSCPSDKICKHVIIAIVYYAENHLKAGAETEEE
                     LETYKPDFSWLLQLQPSHISKAFTPVQLEEVLFRLDYFEEMDIVETSFLTMTLRTQDIS
                     VSFDSESEIGKSMCTCKAKGNCLHKLEVLLRYRAMHQLQDREHLQASVTDVSYETDVVA
                     EAKALIAEILGIGLAKLSQTICTRLELLAIAAHNGNLPRVEKDIRGIHGELNLFFQRHV
                     KFSTEALLDRLSRVYLSLLALEKTDHTEAKKQLLGSFKSKYHMVPQLSLYALGANPWET
                     RSGYKGITYYFYSLFDQRIYTYTEARPVYYEGISFSFKDSYKGKVPWGELISMEELSHS
                     QVILTQAKTNREQRLSSSEETRLTIVPRDNIEELDLGKVFVRDWSSGWKDAVGDMFETA
                     LDSVFLLKAKKFNQVDFDQKTQQLILTIEDEQCHSLQLSISYQGEFARNIRHLEQNKRL
                     LELREVYLLVQKIGDGLYPISIMQGKSLTSFKLDL"
     gene            4428..4724
                     /locus_tag="BP422_RS09025"
                     /old_locus_tag="BP422_08785"
     CDS             4428..4724
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015891016.1"
                     /locus_tag="BP422_RS09025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_08785"
                     /product="hypothetical protein"
                     /protein_id="WP_088907441.1"
                     /transl_table=11
                     /translation="MEELYSFTEKLTDWQERLLLKGIHKLDKQDLQELKKLQELAIEYD
                     MSFLGSLIEELHVEGNRYLQEVKVDAELLTQQYLYVVQYVNMMKKPLSRALSS"
     gene            5001..6935
                     /locus_tag="BP422_RS09030"
                     /old_locus_tag="BP422_08790"
     CDS             5001..6935
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /locus_tag="BP422_RS09030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_08790"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_088907442.1"
                     /sec_met_domain="TIGR03975 (E-value: 6.2e-189, bitscore:
                     627.7, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 3.1e-12, bitscore: 45.7,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIALINMPFGSLYRPSIGLSLLQASLQKNGLVCDTYYLNLLFGS
                     MVTTDLYNYFVEQKNAPEEAFSRDWIFSQSLYGKDNFEDYERYMDQSKFFRSDAKRQEY
                     CERLLSIRDYVEPFLERALADYPWEQYDIIGFTSVFEQNVASLSMAKRLKEKWPNTFII
                     FGGANCEGEMGQALLESFPFLNAVCSGEGDLSFKNFVDQMVEKKDPRLCRSQGIILQST
                     YTDEPLMTRSENSPPVHDMDGLPYPNYDDYFTYFEKYGFNGTKPAPRFLFESSRGCWWG
                     AKSHCRFCGLNGANLNYRSKSADRALEELLFLRERYQKYTNKASAVDNIIDMKYFRDFL
                     PKLRDMQLDLDMFYETKANLRKDQVQLFRDAGFHHIQPGIESLITSVLKLMGKGLSMLQ
                     NVQLLKWTKEFGVYPYWNFLYGFPGENEEEYQKVVDVIQALTHLDPPGICSPIRLDRFS
                     PYFNHASEHGIVNIRPMFAYHLVYQEQSRETLLKLAYHFEFDYASIQNPASYTQALIRE
                     IDSWQKQHSQSEFFSVDLGLHLVLVDLRPIRLENHMLVLTGLEKAIFQFCDSIHSYKGI
                     CEYLEKNQISHTQKDVKDILNRFVQQKWMLTESDMYLSLAVPLGNYSPKKQGLERLIEI
                     SGGLDSKIS"
     gene            6957..7139
                     /locus_tag="BP422_RS09035"
                     /old_locus_tag="BP422_08795"
     CDS             6957..7139
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BP422_RS09035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="BP422_08795"
                     /product="hypothetical protein"
                     /protein_id="WP_088907443.1"
                     /transl_table=11
                     /translation="MSEQKEKEFKLSPKAFAITPGGEVVIDNKQLAEALKNATASNARE
                     DALEGEVTVSVTVKI"
     misc_feature    7329..7343
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.51"
                     /tool="antismash"
     gene            7513..9090
                     /locus_tag="BP422_RS09040"
                     /old_locus_tag="BP422_08800"
                     /pseudo=""
     CDS             7513..9090
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015891017.1"
                     /locus_tag="BP422_RS09040"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_08800"
                     /product="glutathione ABC transporter substrate-binding
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKKKSFYHTLIAMTIALSAAMAGCSSGQDASTSAGQAGGTAEAG"
     gene            9206..9685
                     /gene="ybaK"
                     /locus_tag="BP422_RS09045"
                     /old_locus_tag="BP422_08805"
     CDS             9206..9685
                     /GO_function="GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /GO_process="GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ybaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718015.1"
                     /locus_tag="BP422_RS09045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_08805"
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="WP_088907444.1"
                     /transl_table=11
                     /translation="MKQGKTNAMRILDKEKIAYTMLTYATDDGKIDGIAVAQKIGREEN
                     VVYKTLISQGTSKSYYVFVIPVEAELDLKKAAKAVGEKKIEMISVKEITKVSGYIRGGC
                     SPVGMKKLFPTVIDQRAQSLETIIVSGGNIGVQIEMAVEGLLQATKGSLADVSSI"
     gene            9908..10933
                     /locus_tag="BP422_RS09050"
                     /old_locus_tag="BP422_08810"
     CDS             9908..10933
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016740520.1"
                     /locus_tag="BP422_RS09050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_08810"
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_088907445.1"
                     /transl_table=11
                     /translation="MKSDRIKFPAGFWAGLQRLGIAPQDLVRKASLPLTILTEPAVVTT
                     PQYFRIWQAYSDLVGDIATGIIKLSTTFETAQYPPAVLATYHARYYRDALNRMARYKQL
                     CPPERLRITEEGERCTIELEWMHSEEPGPPVLVGTTLAFLLELGRRGTGKRLTARSVEF
                     SYSMGDVTALEAYFGCPIRIGTTDNRLTLHRRDLDLLFLSYNKELLEILTPVLDRSLNE
                     QCSHSSAEMVKWILKRSLSGGRLDIRAVASELGMSDRTLQRRLTDECTMFKQLLTQARH
                     EQAREYLADPKLDIKEVAFLIGYEDQNSFYRAFRLWEGETPSNWRSEYFRVNPAVIESL
                     T"
     gene            11122..11913
                     /locus_tag="BP422_RS09055"
                     /old_locus_tag="BP422_08815"
     CDS             11122..11913
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016818767.1"
                     /locus_tag="BP422_RS09055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BP422_08815"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_088907446.1"
                     /transl_table=11
                     /translation="MDMGLRNKTALVTGSTKGIGKAIAIELAREGVNVLVNGRNDEEVE
                     RIVNELKSEFPATSPQNATADLVDRQQREALLEKYPHVDILVNSMGIYEIMSYEDVDDE
                     VWEKYFRTNVLAANGLSKFYLPNMLKNNYGRIIFIASEEAIMPSGQMPQYCMTKSMLLS
                     LSKSLSKRTVGTEVTSNTIMPGPTLSENVYQIIDSLFPDDMAFSEKENQFMAANLPQSE
                     LQRFIKPFEIGRLVAFVSSPYATAFKGSPIRMDGGMVPTIF"
ORIGIN
        1 ttgcgagaaa agtattatcc aggagagcag gtcaaggaag gctccctttt gaatcagaag
       61 catttgaatc ggtatatgga actggtcatc agcttgctgt atttccgcat tcacgtgaaa
      121 ggcgggatca gtgcgttcct gaacaaggaa gaggagatcg atcgggagct gcacgatact
      181 ttgcaaatcc ttttcggtgt gtgcccgctt gatatcctgt ccgaagataa gagactggct
      241 gaccttgtgc acatggagga gccttacgat ctactgctgg gacttcgcga gcatttgcta
      301 tccccgccga cgctctggga gcaggtgaaa cagctggtgc ttgaagatgt aaccaaatcg
      361 agacgagcgc ttgagcttgc ttcgatcccg atggtcaaag gatttttata caaaacgctg
      421 gcggcagaag gcgtggatct ctccgatttc cagccttctt tggaagggat tattcaagac
      481 gcgctgcgac gtgcgaaggg ctcggcgaag ctggcgaact atttggatga aaaagctgcc
      541 ctagaggatg tgctcgagca tgtgcatctg gaggacgtac aactcgtaag acagcagatg
      601 atcctgttga gcttcttgcc tgtggatcat ccgtttattg cgcgtctgat tccgttcatg
      661 tgccaataca aagacaagtc caatcgcaga gtaggccata tgtgcttcgc tcatgcgttc
      721 caggatcgac tgaccactat catcgatcat taccggaagg acgaagcggt agatatccag
      781 acacttttct atcagatttt gtccatcaag gagatgcact attattacac agacgtcaat
      841 gaagaggtgt atcgttcgtt ggtcgtagcg aatccggaaa tgagtctggc tcaatttgac
      901 aagctggata cggagcttcg cgtgtttgtg atgcaaaccg tattagcggc aaaggagacg
      961 ctgtctgatg agttgcgcaa ccaagcgatt ctgctcggat tggctgacag ctcaaagaaa
     1021 gtgagtggcc tcgctggtgc agcgtttctt caggccaaag ataaggagct ttatttacac
     1081 gtctatcaaa cggagaaaaa agcaaaggtc aaggaaatgg cacttgatgc gattcgctcc
     1141 ctggagaata acaaggagat tttccaggag cttttgacaa cagaaaagag cagcaaattt
     1201 aaaacgctgc tgcaaacatt tatcgacgct gaggagcaag ggccagatgc gagtttgacc
     1261 catttgggca atctgaccga taagcgaaag ctcacacgga ttaaatggat gccactggag
     1321 cgcatgccgg tccttcgcga ccaagacggc aacgagctgg gtaaagaagt aatggagtac
     1381 atgctaaccg cctcaatcga tttcccgaca gcgccaaacc cgttggtact tgacttgaaa
     1441 ccgtctctgc aagcggcatc tgttgctgac tttactacag aagttttgcg cacatggctc
     1501 gataatggag cagtcgccaa agaaaaatgg gtcatgccgc tctgcgtcgc cttcggagat
     1561 cgacgtatag tcgatacgat tggtcagttg attaaggagt ggacagatta ctcccgcggt
     1621 gctttggctg cggatggcgt acgtgctctg tcgttctcaa atgatttgac tgcgctgcgt
     1681 ctaatcgacc agatcaagcg tacgatcaaa aacaggcaag tcaaatcagc agcagaagag
     1741 gcgctgtcca tggcggctgc gaatcagaac atttccgcga tggaactgga agaccgattg
     1801 gttaccacgc ttggcttcga tgcgtccggc aagcaagtgt ttgactacgg cgaccgcacc
     1861 ttcacggtca aagtcagcaa cgagttggag ctggaagtta caaacgacag cacaggaaaa
     1921 gcggtcaaaa atctgcctgc accgtccgcc aaagacgatc aggagaaggc ggaacaagcg
     1981 cgattgactt ttgctcaatt gaaaaaagac ctgaaaaata tggtgaaagt acaatctctt
     2041 cgtctggagg agtccctgtc caagtcgcgt tattggtcaa cggcagcatg gaaacgtctc
     2101 tttgtggaga acgtgttgat gcaaaaattc gcaatcgggc tcatctgggg tgtatacgag
     2161 gacggaaagc tcgtagatac gttccgctat atggacgatg ggacatttaa caccgtggac
     2221 gaggatgagt atgagttggc agatgagcag cttattggct tgattcatcc actcgagctg
     2281 gacgaagaga cgttgaacgg ctggcgtact cagctggaag attacgaaat tacgcaaccg
     2341 ttcgagcagt tgaatcgtga agcgttcctg ccaaccgaag aagaaataaa agccaatgaa
     2401 ttgctccgcc tgcctgttga atcgtattca cctactgctt ttgcgaaaat gatggagaaa
     2461 ttcggctggg tcaaaggaac gccgctcgat gccggttatt attacgagtt ttacaaactg
     2521 tatgacggta tggtggctga gctgaaaatc agtggggcga gcatttcgta ttgggaagga
     2581 atggaggatg tgaagctgga agcgttggct ttctttccaa ataaagatgc cgagtacaag
     2641 catttttact tcaaaccagt ggatcgactg aagctcactg atattccaac acgcattttc
     2701 agcgaaaccg tctatgatgt catgcgagca gcaggaaaat aacgatggcg gatttcacat
     2761 cagaatttca tcggtttatc gcgctgtgca acgaagagta tttgatcaaa tacgcgaaca
     2821 aaggaatata caaccgttcc atcaaggaaa tggaaaaagg aatgaccgtt tcctacgagt
     2881 tcggggacga ttacgtcgag tgtgcgctgt cagatgacag cgtatgccga cttcatgcag
     2941 atatgaatcg cttttcctgc tcatgtccat cagacaagat atgcaagcat gtcatcattg
     3001 ccatcgtcta ctatgccgaa aaccatttga aagcaggagc agagaccgag gaagagctgg
     3061 agacctacaa gcctgatttt agctggctct tgcaattgca gccgtcccac attagcaagg
     3121 cttttacccc agtacagctg gaagaagtcc tgtttcgact cgactatttc gaggagatgg
     3181 atattgttga aacatctttt ttaaccatga cattgcgcac ccaagatatt tccgtatctt
     3241 ttgacagtga atcggagatc gggaagagca tgtgcacttg caaggcgaag ggcaattgct
     3301 tgcacaagct ggaggtcctt ttgcgctatc gggccatgca tcagctacag gatcgtgagc
     3361 acctgcaagc atccgtaacc gatgtcagct acgaaacaga tgtcgtagcg gaagccaaag
     3421 cgctaatcgc agagattctt ggcatcggct tggcgaagct atcccagacg atttgtacac
     3481 ggctggagtt gcttgctatc gctgcgcata acggcaatct tccccgcgtg gaaaaagaca
     3541 ttcgcggcat tcatggggag ttgaacttat tttttcagcg acatgtcaag ttttccacgg
     3601 aagcgctgct cgaccgcctt tcccgggtgt atctcagttt gcttgctcta gagaagactg
     3661 atcacaccga agcgaaaaag cagctgctcg gcagcttcaa aagtaagtac catatggttc
     3721 cgcagttaag tctgtacgct ttgggagcaa atccctggga gacgcggtca gggtacaagg
     3781 ggattacgta ttacttttat tcgctttttg atcaacgcat ctatacgtat actgaagcgc
     3841 gccccgtgta ttatgaaggc atatcctttt cttttaagga cagctataaa ggtaaggtac
     3901 cctggggcga gttgatctcc atggaggagc tctctcattc acaggtcatc ctgacacagg
     3961 cgaagacgaa tcgggagcaa cgcttgtctt ccagtgaaga gacgcggcta accatcgttc
     4021 ccagggacaa tattgaagag ctggatcttg gcaaggtgtt cgttcgcgac tggtcaagtg
     4081 gttggaagga cgcggtagga gatatgtttg agactgcgtt ggactcggtt tttctattga
     4141 aagcgaagaa gtttaaccaa gtcgatttcg atcagaagac gcagcagttg attttgacga
     4201 tcgaagacga gcaatgtcat tctctccaat tgagcatctc ctatcagggg gaattcgccc
     4261 gcaatatacg tcatctcgaa caaaacaaac ggctgctgga gttgcgcgaa gtgtatttgc
     4321 ttgtgcaaaa aataggagat gggctttatc cgatcagcat catgcaggga aaatcgttga
     4381 cgagcttcaa gttagaccta tgacggtgag ggagtggacg tgaaatcatg gaggagttat
     4441 attcctttac cgaaaagctg actgattggc aggaacgctt gttactcaaa ggaatccata
     4501 aacttgataa gcaagatttg caggaactga aaaagctgca agagctcgcg atcgaatacg
     4561 atatgagctt cctcggctca ttgatagaag aactacatgt ggagggcaat cgctatttgc
     4621 aggaagtaaa agtggatgcg gagttgttaa cgcagcagta tttgtatgtg gtgcagtacg
     4681 tcaatatgat gaaaaagcct ctatcgcggg cattgtcgtc ataagcatga agtttgactc
     4741 gatttagaag gcagtacatt acaactgttt acatgattcg aaaataagag aggactcgtc
     4801 aggccgttgc ccaggcgggt tttttcgttt tttcctaaat tatctttaca agtgggactt
     4861 agttttccga tggcctctaa ttatgtgatc tgatgatcct ggtatattgg agataaaaag
     4921 tggttaagat gatgaatata actcccaaat tttaccacat agagtatata tatatggaaa
     4981 agaaaggttg atttttccgc atgaaaattg ctctaatcaa tatgccgttt ggatccttat
     5041 atagaccatc tattggtctc tctctcttac aggcatcctt acaaaaaaat ggcctggtct
     5101 gtgatacata ctatttgaat ttgttgtttg gttccatggt aacaacagat ttatataact
     5161 attttgttga gcaaaaaaat gcacctgaag aggcgttttc cagagactgg atattctctc
     5221 agtctttata tggaaaggat aatttcgaag attatgaacg atatatggat cagtccaaat
     5281 tctttcgatc agacgccaag cgtcaagagt attgtgaacg attattatca atacgtgatt
     5341 atgtagagcc cttcttggag cgggcgcttg cagactatcc atgggagcaa tatgacatta
     5401 ttggatttac ctcagttttt gaacaaaatg ttgcttcatt aagtatggca aaaagattga
     5461 aagagaaatg gccaaataca tttattattt ttggtggagc taattgtgag ggggaaatgg
     5521 ggcaagcgct cttggagtct tttcctttcc taaatgctgt ttgctctggg gaaggtgacc
     5581 tatcatttaa aaattttgtt gatcagatgg tagaaaagaa agatcctcgg ctatgtcgtt
     5641 cccaggggat tatcctccaa tcaacataca cagatgaacc cttaatgacg cgttctgaaa
     5701 attctcctcc cgttcatgat atggatggat tgccttatcc caactatgac gattacttta
     5761 catactttga aaaatatgga tttaacggaa cgaaaccagc tccacggttt ctttttgaat
     5821 cctcaagagg ttgctggtgg ggggctaagt cacactgtcg tttctgcgga ttaaacggag
     5881 caaatttaaa ttatcggagt aaatcagcgg atcgagcatt agaagagctt ctttttttgc
     5941 gagagcgtta tcagaagtat acaaacaaag cgtcagcagt cgataacatc attgacatga
     6001 aatatttccg tgatttcctg cccaaacttc gcgatatgca attggacttg gacatgttct
     6061 atgagacaaa ggctaatttg cggaaagatc aggttcagtt gtttcgggat gcgggttttc
     6121 atcatattca accaggtatt gagagcttaa ttacttccgt tctcaagctg atgggtaaag
     6181 ggctcagcat gctgcaaaac gttcaactgc ttaaatggac caaagaattt ggtgtatatc
     6241 catattggaa ttttttgtat ggctttcctg gtgaaaatga agaggaatat caaaaagtgg
     6301 ttgatgtgat tcaagcgctc actcatctgg atccaccagg aatttgctcg cccattcgtt
     6361 tagatcgatt tagtccatac tttaatcatg cctcagagca tggcatcgtg aatattaggc
     6421 cgatgtttgc ttatcatctt gtttatcagg aacaaagtcg tgaaacactt ttgaaactcg
     6481 cgtaccattt tgaatttgat tatgcgtcga tacagaatcc tgcatcctat acgcaggcat
     6541 tgattcgaga gatagattct tggcaaaagc aacatagcca aagcgagttt ttttcggttg
     6601 atcttgggct acatcttgtt ttagtagatt tgaggccgat tcgattggaa aaccatatgc
     6661 ttgtactaac gggtttggaa aaagctattt tccaattctg tgatagcatt cactcgtata
     6721 aaggtatctg cgagtatttg gagaaaaatc aaatttccca tacccaaaaa gatgtcaaag
     6781 atatactgaa tcgatttgtt caacagaaat ggatgctgac agaatcggac atgtatttga
     6841 gtttggccgt ccctctaggg aactattccc ctaaaaaaca gggtttagag cgactgatag
     6901 aaatttcagg tggtctagac agcaaaattt cgtagatgag taaaggaggt gaaaaaatga
     6961 gtgaacaaaa ggaaaaagaa tttaaactgt ctccaaaagc tttcgccatt acaccagggg
     7021 gagaagtagt catcgataat aagcaattag cggaagcttt aaagaatgca acagcttcta
     7081 atgctaggga agatgctctg gaaggtgaag ttactgtatc tgttacagta aaaatatgag
     7141 cggtacaggt gtgttagtgt aaagtaaacg ttaatatgta cccttggttt aaggccataa
     7201 aataatctca cacaaaatgt tgtgtgagat tattttacta tgcaactaac atgagcggcg
     7261 gggtacgacc ttttgcttca tggatatggc ggcttgaaga gtatcacaag tgagccaaac
     7321 ggaagtgaat ttgtcagata atcgttgaat cactctctcg ctaccatagg ggagtgtttt
     7381 ttgtttgtat ataaagttgt aaagaaactg aaaagaaagg taaacaaagt gaaaatacga
     7441 tgactgaata ttgtctaaaa taaaacatgt ttgaatgcta aaacatttgc aacaagaatt
     7501 gggggaacac agatgaaaaa gaagagcttt tatcacacgc tgattgccat gacaatcgcg
     7561 ttatctgcgg ctatggctgg gtgttcgtcg gggcaggatg cgtcgactag cgccggacaa
     7621 gcagggggta ctgcggaagc tggctaaaca ggaggaacac tcgtcatcgc gcgtctgtcc
     7681 gatgcgaaca acctcgatcc gcatttcctg tctcaaatcc cttcagcagc aatcattcat
     7741 cacaaagtat acgaagggct tgtccgcatg gataaagaaa gcaaatacgt cccatccctg
     7801 gcaagtgagt ggaagcagct caatgacttg acgtgggagt tcaagcttcg tcaagacgtg
     7861 acgttccatg atggcgcacc tttcaatgcg gaagcggtaa aagcaacgat cgctcgcgtt
     7921 caagaccctg ccgttggctc cagccggatt aatatgtttg aagcaatcag ggaagtgaaa
     7981 gtagtagatg agtacacggt ataatttatt ctgaattacc cgtacgcacc gatcttatct
     8041 gttttggcga gtgcagaggg aagcatcatc agtccgaaag ccattgaaca atatggaaaa
     8101 gaattgagca agcatccaac tggtacgggc ccgtacaaat tcgaaaaatg gacgccgggt
     8161 caagaagtgg ttctcgtgag aaacgatagc tactatggcg gtacaccaaa tttggataaa
     8221 gttgttttca aaaccgtacc ggaagacaca actcgccttg ccatggttga gacaggagaa
     8281 gttcaagtgg cggaaaacct gcctgtgacg gacatcgatc gcgtacaaaa ttctccatcc
     8341 atgcaattgg gtcgataccc tgggttctct gtcgatcaca ttggattgaa tacaaagaaa
     8401 aagccgtttg atgatgtgcg tgtccgccaa gccattgccc acgcaatcga caaaaagacc
     8461 attattgaag gcgtttacaa cagtgtggga acaccagcgc actcttcgat tacaccagcg
     8521 atggttgggt acagcccgaa tgtaaaagac attccgtatg acgtcgtgaa agcgaaacaa
     8581 ttactcgcag aggctggata tccaaacggc ttcaaagcaa aaatcgcatt aaacgacaac
     8641 aaggcacgaa ttagcgtagc cgaggttttg cagcaacagc tgaagccaat cggtattgac
     8701 ctgcaactgg atgttatgga attcggggct tatatcgatg cagcatccaa aggggagaca
     8761 gacctgttca tgagtggatg gggaaatgct acgggcgatg ctgattacaa ccaatcgaac
     8821 ctatatcact cgaaagcaca tggcgcacct ggtaaccatt ctttctacaa caacccggaa
     8881 gtagacaagc tgattgacga aggacgccga gaaacagacg cagaaaaacg gaagcaactc
     8941 tatgaaaaag cacaacaaat cgaaatgaac gaagctccgc ttattccgtt ccgattctcc
     9001 gagaccttgg cggccattca aaagaatgta cagggtgtat ggattagccc tgcaggccat
     9061 atcgagattg acgaagtaac gattcaataa tacgaggcgc cactggcatg cttgtgtcag
     9121 tggcgttttt tagttgaaaa atagaacacc atcccctaag atagattata ttttgttgac
     9181 cagcgtgtgg cggaggtgga atggcgtgaa gcaaggaaaa acgaatgcaa tgcggatatt
     9241 ggacaaagaa aaaatcgcat acaccatgct gacatatgca acggatgatg gaaaaatcga
     9301 cggaatcgct gtcgcccaaa agatcggtcg cgaagaaaac gttgtctata aaacattgat
     9361 ttcgcaaggg acgagcaaat cgtattatgt gtttgtcatt cccgttgaag ccgagcttga
     9421 cctcaaaaaa gcggcgaaag cagtcggtga gaagaagatc gagatgattt ccgtgaaaga
     9481 gatcaccaag gtttccggct atattcgtgg tggatgttcg ccagttggga tgaagaaatt
     9541 gtttccgacg gtgatcgatc aacgagcaca atcgctagag acgatcatcg tcagtggcgg
     9601 aaacattggg gttcagatcg agatggcagt tgagggcttg ctacaagcaa cgaaaggctc
     9661 cttggcagat gtatcttcaa tctaaagcaa aaaggccacg gcattagctg gggccttttt
     9721 gctgtttatg gaaaaagtcc cgtccaaact tggatagggg tgtcgcatga ggctctagcg
     9781 aaaatagacg ggattgaaga aatcatcaaa gcgatgggca agcaaaaata acggatacga
     9841 tcaccttcat gtatacttaa gcataatcgg aacgaatcgg taagcaacta aaggagatcc
     9901 atacgtaatg aaatcagacc gtataaaatt cccggctgga ttttgggcgg gattacaacg
     9961 actagggatt gccccccaag acctagtacg taaagcaagt ctgccgctca ccattcttac
    10021 agaaccagct gtagtcacca ctccccaata tttcagaatc tggcaagcgt attccgatct
    10081 agttggtgac attgctaccg gaatcatcaa gctttccacc acatttgaaa cagcacaata
    10141 cccaccggct gttttagcga cctaccatgc tcgttactat cgcgacgcac ttaatcgaat
    10201 ggcccggtac aaacaactgt gccctccgga aaggttgcgt ataacagagg agggcgagcg
    10261 ctgtacaatc gaattggagt ggatgcatag cgaggaaccc ggaccaccag tcctggttgg
    10321 aaccacgctc gcctttcttt tagaacttgg gcgacggggc acgggcaagc gattaactgc
    10381 aaggtccgtg gagttttctt actcgatggg agatgtaact gctcttgaag cttactttgg
    10441 ttgccctata cggattggca ccaccgataa ccgattaaca ctccatcgac gagacctgga
    10501 cctcctcttt ctttcgtata acaaagaatt gctggagata ttgacacctg tattggaccg
    10561 atcattgaat gaacagtgca gtcattccag tgctgagatg gtcaagtgga tcttgaagcg
    10621 aagtctatcc ggaggccgcc ttgatattcg agcggttgcc agcgagctgg gcatgagcga
    10681 tcgtactttg cagcgccgtc ttacggatga atgcacaatg ttcaagcaac tgttaacaca
    10741 ggctcggcat gaacaggccc gagaatactt ggccgacccc aagctcgata ttaaagaagt
    10801 ggctttctta attggatatg aagatcagaa ctcattttac cgggcctttc gcctttggga
    10861 aggcgaaaca ccttcaaatt ggcgttccga atactttcgg gtcaatcccg ccgtcataga
    10921 atccctaacc taacccgttc ctagcagggc tagcattttc ctgatttcca tatttggcgg
    10981 gtgaagcaag aaatttggcg tagtacgcta gttacccaac gatccagaca aagtacgata
    11041 atctctatcc ggagcacaag ggtgttccaa gatgaacttc tagtcagctt tccgtgaatg
    11101 actacaaagg agacaagtgt tatggatatg ggattaagga acaaaactgc tttagtaact
    11161 ggatcgacga aagggattgg gaaagcaatt gccattgagc ttgccagaga aggtgtcaat
    11221 gtcctcgtaa atggacgaaa tgatgaagag gtagaacgaa tcgtaaatga attgaagtcg
    11281 gaattcccgg ctacttctcc tcagaatgct acggccgatc ttgtggatag gcagcaaaga
    11341 gaagctttat tggagaaata tccccatgtg gacattctcg ttaatagtat gggcatttat
    11401 gaaatcatgt cctatgaaga cgttgacgat gaagtatggg aaaaatactt ccgaacaaat
    11461 gtccttgccg cgaatggatt atctaaattt tacttgccta acatgttgaa gaacaattat
    11521 ggacgcatca tctttatagc aagtgaagaa gcgatcatgc cttcagggca aatgcctcag
    11581 tattgcatga caaaatcgat gctattatca ctgtcaaaaa gtctatctaa acgaacagta
    11641 ggaacagaag tcacctccaa tacaatcatg ccaggaccaa cgctttccga aaatgtctat
    11701 caaatcatcg atagcttgtt tccagatgat atggcatttt cggaaaaaga gaatcaattt
    11761 atggctgcaa acctgcccca atccgaatta cagcggttta tcaagccttt tgaaataggc
    11821 aggctggtag cattcgtatc cagcccatat gcaaccgcat ttaaaggttc accaatccgt
    11881 atggacgggg ggatggtgcc aactattttt taaattgcga tcagaattgc taata
//
