LOCUS       NZ_CP022161            10399 bp    DNA     linear   CON 15-DEC-2024
DEFINITION  Streptomyces capitiformicae strain 1H-SSA4 chromosome.
ACCESSION   NZ_CP022161
VERSION     NZ_CP022161.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces capitiformicae
  ORGANISM  Streptomyces capitiformicae
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10989391)
  AUTHORS   Liu,C.
  TITLE     Streptomyces capitiformicae sp. nov., a novel actinomycete producing
            naphthoquinone compounds isolated from the head of Camponotus
            japonicus Mayr
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10989391)
  AUTHORS   Liu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUN-2017) Life of Science, Northeast Agricultural
            University, No. 59 Mucai Street, Harbin 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. Feb-2017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002214185.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 14:26:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,013
            CDSs (total)                      :: 9,935
            Genes (coding)                    :: 9,274
            CDSs (with protein)               :: 9,274
            Genes (RNA)                       :: 78
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 661
            CDSs (without protein)            :: 661
            Pseudo Genes (ambiguous residues) :: 0 of 661
            Pseudo Genes (frameshifted)       :: 220 of 661
            Pseudo Genes (incomplete)         :: 571 of 661
            Pseudo Genes (internal stop)      :: 32 of 661
            Pseudo Genes (multiple problems)  :: 155 of 661
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:06:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5315380
            Orig. end                         :: 5325779
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022161.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="22"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            96..815
                     /locus_tag="CES90_RS23705"
     CDS             96..815
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381151.1"
                     /locus_tag="CES90_RS23705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_189783714.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDGVEVVHRSFELDP
                     GRAKDDIQPVLTMLSKKYGMSEAQAQAGEYNLREQAAAEGLDYRAEGRDHGSTFDMHRL
                     LHFAKEQGRQSELLQLFYRANFAEERSVYAGADEFLIELAVEAGLDGEAARKVLADPSA
                     YADAVRADERQAAELGANGVPFFVLDRKYGVSGAQPAEVFERALVQAWGERPPLTVVQG
                     DGAEVCGPDGCAVPQQG"
     gene            923..1972
                     /locus_tag="CES90_RS23710"
     CDS             923..1972
                     /NRPS_PKS="Domain: Aminotran_5 (16-342). E-value: 5.3e-26.
                     Score: 83.3. Matches aSDomain:
                     nrpspksdomains_CES90_RS23710_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436207.1"
                     /locus_tag="CES90_RS23710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_229913906.1"
                     /sec_met_domain="Aminotran_5 (E-value: 6.4e-19, bitscore:
                     67.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFESLVSTEFAPKNTYLNTASTGLLPARAVDAMKAAVESVADG
                     RPQDMFADVEAARAAFARIVGVPDRRVAAGASVAVYTGLIAASLPSGAEVVTAEGDFTS
                     VVTPFHVRGDLKVRQVPLERVAEAVRPGTTLVAVSVAQSADGRVADLEAIREAARAHGA
                     RTYVDASQSAGWLPIEADAYDYVVSVGFKWLVCPRGVAFLVVPEDLGGLTPVFAGWVAG
                     ERPWDSCYGPVEELAHSARRFDESPSLFSYAGARRSLALIEELGVANVRDHDLVLADRF
                     RAGLAGLGHAPVPAPDSAIVSVPGLGHRQAELSRAGVEVSDRAGHLRAAFHLYNTPADV
                     DRILDVLSA"
     aSDomain        971..1948
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CES90_RS23710_Aminotran_5.1"
                     /evalue="5.30E-26"
                     /label="CES90_RS23710_Aminotran_5.1"
                     /locus_tag="CES90_RS23710"
                     /protein_end="342"
                     /protein_start="16"
                     /score="83.3"
                     /tool="antismash"
                     /translation="TYLNTASTGLLPARAVDAMKAAVESVADGRPQDMFADVEAARAAF
                     ARIVGVPDRRVAAGASVAVYTGLIAASLPSGAEVVTAEGDFTSVVTPFHVRGDLKVRQV
                     PLERVAEAVRPGTTLVAVSVAQSADGRVADLEAIREAARAHGARTYVDASQSAGWLPIE
                     ADAYDYVVSVGFKWLVCPRGVAFLVVPEDLGGLTPVFAGWVAGERPWDSCYGPVEELAH
                     SARRFDESPSLFSYAGARRSLALIEELGVANVRDHDLVLADRFRAGLAGLGHAPVPAPD
                     SAIVSVPGLGHRQAELSRAGVEVSDRAGHLRAAFHLYNTPADVDR"
     gene            2083..2691
                     /locus_tag="CES90_RS23715"
     CDS             2083..2691
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477737.1"
                     /locus_tag="CES90_RS23715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_189783716.1"
                     /transl_table=11
                     /translation="MVVPVEPTLEQPSIDAELHRRLVYGDESALREAYATYGGLVRRVA
                     VRVTHSTASAEDVAQEVFAQLWSRPYGFDPRRGSLRTWLSMLAHRRAVDWVRSEARHRK
                     DARADDSALAAIPDTGPGPDEAVVDRERSLLLHTALAELPRTQREVVHLAYFAGRTYRQ
                     AAVELGIPEGTAKTRLRSALRKLAESLADPPDPAPVRGA"
     gene            2768..3793
                     /locus_tag="CES90_RS23720"
     CDS             2768..3793
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477735.1"
                     /locus_tag="CES90_RS23720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="WP_189783717.1"
                     /transl_table=11
                     /translation="MTNDHDGVRELLAAWAVGALPPGDDRTVPLHLAGCESCATEAERL
                     RHTVRLLDGGPSDGRPPASALNGSADGALAMALRSRGPRTPGVAPHAAPYAAAVAGLQA
                     LIPELDGRWGTPVVHDWDAHATVAHLIAADEHLAVALGVASRLPASHIPDGTSAGDAWD
                     RRTADVIAHEHTRTPEETVATWAAQAAALLATPEARDTELAARAITVMDLRLPIADHFV
                     IRAFEAWIHTDDIGRALGLPVPPPPDEHLDQLVRLAVRILGLALHDAPPVLFEVTGPTD
                     SSWVLGSGDDPVIAELTLDPVDFCLLVGGRYAPAEVPRGETGDAGAVRNVLRTAAALSW
                     L"
     gene            complement(4108..4995)
                     /gene="thpD"
                     /locus_tag="CES90_RS23725"
     CDS             complement(4108..4995)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013004613.1"
                     /locus_tag="CES90_RS23725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_189783718.1"
                     /sec_met_domain="PhyH (E-value: 8.2e-41, bitscore: 139.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTITDLYPSRGASEVTVPRQDPVVWGAPGTPGPASLTDLQAYER
                     DGFLAVEELITPDEVAVYRQELDRIITDPAIRADERSIIEPKSKEIRSVFEVHRISELF
                     ARLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNMRT
                     VSVSIALTENYDTNGGLMIMPGSHRTFLGCAGATPKDNYKKSLQMQDAGTPSDEALTAM
                     ATEYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENAAVEPFAAPVR
                     RPTFIGARDFTPVP"
     gene            complement(5001..5399)
                     /locus_tag="CES90_RS23730"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="CES90_RS23730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_189783719.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.8e-54, bitscore:
                     182.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKSASGTWESKRIVLAKEKVGFSLHETILY
                     AGTETSMWYANHIEAVVCVEGEAELTDDETGRKYTITPGTMYLLDGHERHTLRVKEDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEG"
     gene            complement(5457..6737)
                     /gene="ectB"
                     /locus_tag="CES90_RS23735"
     CDS             complement(5457..6737)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 1.9e-74.
                     Score: 242.6. Matches aSDomain:
                     nrpspksdomains_CES90_RS23735_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="CES90_RS23735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_189783720.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.1e-78, bitscore:
                     261.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAHGSRMYDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTTAKRAFLESFQNLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVEDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALADLCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTATAALETYWSDGSAMEKQTRARGEQIEQAL
                     ISITEENLAEVKEYRGRGLVWGIEFHDKARAERVARRAFALGLLIETSGPEGEVVKLLP
                     ALTITPDELDEGLRTLARAVRETVREG"
     aSDomain        complement(5640..6653)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CES90_RS23735_Aminotran_3.1"
                     /evalue="1.90E-74"
                     /label="CES90_RS23735_Aminotran_3.1"
                     /locus_tag="CES90_RS23735"
                     /protein_end="366"
                     /protein_start="28"
                     /score="242.6"
                     /tool="antismash"
                     /translation="FDRAHGSRMYDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTTAKRAFLESFQNLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVEDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALADLCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TATAALETYWSDGSAMEKQTRARGEQIEQALISITEENLAEVKEYRGRGLVWGIEFH"
     gene            complement(6815..7354)
                     /gene="ectA"
                     /locus_tag="CES90_RS23740"
     CDS             complement(6815..7354)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337972.1"
                     /locus_tag="CES90_RS23740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_189783721.1"
                     /transl_table=11
                     /translation="MTAAQAEAAQAELKIPEGIRIDRPDVTDGAALWRIAGDSGTLDLN
                     SSYSYLLWCRDFAATSAVARDESGEPVGFVTGYVRPERPDTLLVWQVAVDEAHRGRGLA
                     AALLDGLTARVARQQTLTTVETTITPGNTASERLFAAYAERHGAGIERTVLFEAGDFPD
                     GSHQPEVLHRIGPLAV"
     misc_feature    7493..7509
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.14"
                     /tool="antismash"
     misc_feature    complement(7511..7526)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7659..7674
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.15"
                     /tool="antismash"
     gene            complement(7719..8288)
                     /locus_tag="CES90_RS23745"
     CDS             complement(7719..8288)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338019.1"
                     /locus_tag="CES90_RS23745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DinB family protein"
                     /protein_id="WP_229913911.1"
                     /transl_table=11
                     /translation="MTEPRSTGTTPESAPVPVPATLPDGRPIPLMTGDEGPMLESWLEF
                     HRATLELKCAGLDDAQARTASAEPSELTLLGLVQHLTEVERNWFQRVFAGVDAPLVYEE
                     GTGYRLSPERGLDEALYIWRREVARSRATVAGRALDSLGRIPDGQMMGGLAVSLRWILV
                     HMIEEYARHNGHADIVRERIDGVTGF"
     gene            complement(8345..>9079)
                     /locus_tag="CES90_RS23750"
                     /pseudo=""
     CDS             complement(8345..>9079)
                     /NRPS_PKS="Domain: Aminotran_5 (0-171). E-value: 4.7e-20.
                     Score: 63.7. Matches aSDomain:
                     nrpspksdomains_CES90_RS23750_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238578.1"
                     /locus_tag="CES90_RS23750"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /pseudo=""
                     /sec_met_domain="Aminotran_5 (E-value: 7.2e-14, bitscore:
                     50.7, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="NPVAEIGEVVREHGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQ
                     KAMGGPAGVSAISVSERAWARMAANPRAPRRSYLSLLDWKERWIDGGRRALLHAPAQLE
                     MLALEACLERIEAAGPDTVMSRHASAAAATRAGALALGGGLEPYVYEARDAAPVATTLR
                     VPAGVDASELVARALAVDPARPLAAGGGALAKEMVRVNHYGPDASPEAVHGSLAGLGAA
                     MAEAGLTVDPDGARRAASETWG"
     aSDomain        complement(8564..9076)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CES90_RS23750_Aminotran_5.1"
                     /evalue="4.70E-20"
                     /label="CES90_RS23750_Aminotran_5.1"
                     /locus_tag="CES90_RS23750"
                     /protein_end="171"
                     /protein_start="0"
                     /score="63.7"
                     /tool="antismash"
                     /translation="NPVAEIGEVVREHGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQ
                     KAMGGPAGVSAISVSERAWARMAANPRAPRRSYLSLLDWKERWIDGGRRALLHAPAQLE
                     MLALEACLERIEAAGPDTVMSRHASAAAATRAGALALGGGLEPYVYEARDAAPVATTLR
                     VPAGVDAS"
     gene            complement(9360..9776)
                     /locus_tag="CES90_RS23755"
     CDS             complement(9360..9776)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323226.1"
                     /locus_tag="CES90_RS23755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="WP_189783287.1"
                     /transl_table=11
                     /translation="MLRVLMYLVPLALTIYAFIDCLNTPEDEAKHLPKIAWVFIILLFW
                     IVGPIAWLAAGKMRHAPANGRTPSEWHRHHRLEYVAPDDNPEFLKSLAEDNKKDESLLK
                     SWEADLRRREEELKRQEDEKRANREKKNNKDGEE"
ORIGIN
        1 ggagcgtgcc aggcgtcggg cggcaggcgg gactttcgca acacgcccta gtgacccata
       61 gacccatacc cgtacgaccg aagagagctg acaccatgcg cgtcgagatc tggagcgaca
      121 tcgcctgccc ctggtgctac gtggggaagg cccgctttga gaaggcgctc gcggccttcc
      181 cgcaccgtga cggcgtcgag gtggtgcacc gctccttcga gctcgacccg ggacgcgcca
      241 aggacgacat ccagccggtg ctcaccatgc tgagcaagaa gtacgggatg agcgaggccc
      301 aggcccaggc cggcgagtac aacctccgcg agcaggccgc cgccgagggg ctcgactacc
      361 gtgcggaggg ccgtgaccac ggcagcacct tcgacatgca ccggctgctc cacttcgcca
      421 aggagcaggg ccggcagagc gagctgctcc agctcttcta ccgggcgaac ttcgccgagg
      481 agcggtccgt gtacgccggc gccgacgagt tcctgatcga gctggccgtc gaggcggggc
      541 tggacgggga ggcggcgcgc aaggttctcg ccgacccttc cgcgtacgcc gatgccgtcc
      601 gcgcggatga gcggcaggcc gcggagctgg gtgcgaacgg ggtgccgttc ttcgttctgg
      661 accgcaagta cggggtttcc ggggctcagc ccgcggaggt ctttgagcgg gcgctggtcc
      721 aggcgtgggg agagaggccg ccgttgactg ttgtccaggg ggacggtgcc gaggtttgtg
      781 gccccgatgg ctgtgccgta ccgcagcagg gctgagagcc gtcccgtagc cccagctccc
      841 aggcgatgta taagcatcgc ttgggaaaac cacgtaaaac atctcaatgg acggggagga
      901 ctccgggccc cacagtggtt ccatggagac cttcgagagc ctcgtcagta ccgagttcgc
      961 cccgaagaac acctatctga acaccgcgag caccgggctg ttgccggccc gcgcggtgga
     1021 cgcgatgaag gccgctgtcg agtccgtcgc ggacgggcgg ccgcaggaca tgttcgctga
     1081 tgtggaggcc gcgcgggcgg ccttcgcgcg gatcgtcggg gtgccggacc gccgggtcgc
     1141 ggccggggcc tccgtcgccg tctacaccgg gctgatcgcc gcctcgttgc cttcgggcgc
     1201 cgaggtggtc acggccgagg gggacttcac ctcggtcgtg actcccttcc atgtgcgtgg
     1261 tgacctcaag gtgcggcagg tgccgctgga gcgggtcgcc gaggcggtgc ggcctggtac
     1321 cacgctcgtc gccgtcagcg tcgcgcagtc cgcggacggc cgggtcgcgg acctggaggc
     1381 gatccgggag gccgcgcggg cgcacggggc gcgtacgtac gtcgacgcgt cccagtccgc
     1441 cgggtggctg ccgatcgagg cggacgcgta cgactacgtc gtctctgtcg gcttcaagtg
     1501 gctcgtctgc ccgcgcggcg tggccttcct ggtcgttccg gaggacctcg gcggactgac
     1561 cccggtcttc gccgggtggg tcgccgggga gcgcccctgg gacagctgct acggcccggt
     1621 cgaggaactc gcccactccg cccgccggtt cgacgagagt ccgagcctgt tctcgtacgc
     1681 cggagcccgg cggtccctcg cgctgatcga ggagttgggc gtggcgaacg tccgggatca
     1741 cgatctcgtg ctggccgacc gtttccgcgc gggcctcgcc ggactggggc acgcgccggt
     1801 gccggcgccc gactcggcca tcgtgtccgt ccccggactc ggccaccggc aggccgaact
     1861 gagccgtgcg ggggtcgagg tctccgaccg cgcgggccat ctgcgggccg cgttccacct
     1921 gtacaacacg cctgcggacg tggaccggat cctggacgtc ctgtccgcct gacgaccggc
     1981 cgacgtccgg ctgacaccag acggacttcc ctgccgaacc cggctggact cccgtgaccc
     2041 actggcgcgg accgcgcggg gacgctagga aggcttcacg aggtggtggt gccggtggag
     2101 ccgacgctcg aacagccgtc catcgatgcg gagttgcatc ggcggctggt gtacggggac
     2161 gagtccgcgt tgcgtgaggc gtacgcgacg tacgggggac tcgtccggcg ggtggccgtc
     2221 cgggtcaccc acagcacggc gtcggccgag gacgtggcgc aggaggtctt cgcgcagctg
     2281 tggagcaggc cgtacggctt cgacccgcgc cggggctcgc tgcgcacctg gctgtccatg
     2341 ctcgcccacc ggcgggccgt ggactgggta cgcagcgagg cccggcaccg caaggacgcc
     2401 cgtgcggacg actcggcgct cgccgccatc cccgacaccg gacccggccc ggacgaggcg
     2461 gtcgtcgacc gcgagcgctc cctcctgctg cacaccgccc tggccgaact cccgcgcacc
     2521 cagcgggagg tggtccacct cgcctacttc gcgggccgca cctaccgcca ggccgccgtg
     2581 gaactgggca tacccgaggg caccgcgaag acccggctgc gctcggccct gcgcaaactg
     2641 gcggagtccc tcgcggatcc acccgacccg gcgcctgtga ggggcgcgtg atgggggcgt
     2701 gcgggatgta cccggacata cgggccgtgc gtatcgtggg accggcaggg cgaaagggcg
     2761 gcgcgcgatg accaacgacc acgacggcgt acgggagctg ctggccgcct gggcggtcgg
     2821 cgcgcttcca cccggcgacg accggacggt cccgctgcac ctggccgggt gcgagagctg
     2881 cgcgacggag gccgagcggc tccggcacac tgtacgactg ctggacggag ggccgtcgga
     2941 tggccgaccg ccggcgagtg ccttgaacgg gagcgccgac ggcgccctgg ccatggctct
     3001 ccgctcccgc ggcccccgca cgcccggggt cgccccgcac gcggcgccgt acgccgccgc
     3061 cgtggccgga ctccaggcgc tgataccgga gttggacggc cgctggggta cgccggtcgt
     3121 gcacgactgg gacgcgcacg ccaccgtcgc gcacctcatc gcggccgacg aacacctcgc
     3181 cgtggcactc ggcgtcgcat cgcgtctgcc ggcctcgcac atccccgacg ggacctccgc
     3241 gggcgacgcc tgggaccggc gcacagccga cgtcatcgcc cacgagcaca cgcgcacgcc
     3301 cgaggagacc gtcgccacct gggccgcgca ggccgccgca ctgctggcca cacccgaggc
     3361 ccgggacacc gaactcgccg cgcgcgccat caccgtgatg gacctgcggc tccccatcgc
     3421 cgaccacttc gtgatccgcg ccttcgaggc ctggatccac accgacgaca tcggccgcgc
     3481 cctgggcctc cccgtgccgc caccgcccga cgagcacctg gaccaactgg tccgcctcgc
     3541 cgtccgcatc ctcggcctgg ccctgcacga cgcgcccccg gtcctcttcg aggtcaccgg
     3601 cccgaccgac agcagctggg tcctcggctc cggggacgac cccgtcatcg ccgaactgac
     3661 cctggacccg gtcgacttct gcctcctggt cggcggccgc tacgccccgg ctgaggtacc
     3721 gaggggcgag acgggcgacg cgggggcagt gcggaacgta ctgcggacgg cggctgcgct
     3781 gtcgtggctg tgaggccgtg agcacaagac agggccgggc ggtctctcag ggagcagaga
     3841 ccgcccggcc cggtcgcact ggacacgagg tcacctcacc ggagtgaagt ccctcgcctc
     3901 gatgctgcgt tctccggggg cgacccccgg acccccggcc gtgctcggct cacgggacgg
     3961 gagtgaagtc cctcgcctcg atgctgcgtt ctccgggggc gacccccgga cccccggccg
     4021 tgctcggctc acgggacggg agtgaagtcc ctcgcctcga tgctgcgttc tccgggggcg
     4081 acccccggac ccccggccgt gctcgactca cgggacagga gtgaaatccc tcgccccgat
     4141 gaacgtcggt cgccgtaccg gcgccgcgaa cggctcgacc gccgcgttct ccacgctgtt
     4201 gaacacgatg aagacgttgc tccgggggaa gggcgtgatg ttgtcgccgg agccgtgcat
     4261 gcagttgcag tcgaaccagg tcgccgaacc ggccttgccc gtgaagagct tgatgccgta
     4321 ctcggtcgcc atggcggtga gggcctcgtc cgacggggtg cccgcgtcct gcatctggag
     4381 agacttcttg tagttgtcct tcggcgtcgc cccggcacag ccgaggaacg tccggtgcga
     4441 ccccggcatg atcatgaggc cgccgttggt gtcgtagttc tcggtcaggg cgatcgagac
     4501 ggacaccgtg cgcatgttcg gcaggccgtc ctcggcgtgc caggtctcga agtccgagtg
     4561 ccagtagaag ccggacgcgc cgaagcccgg cttcacgttg atccgcgact ggtggacgta
     4621 cacgtccgag ccgaggatct gcctggcccg cccgacgacc cgctcgtcgc gcaccagccg
     4681 tgcgaacagc tcactgatcc ggtgcacctc gaagaccgac cggatctcct tggacttcgg
     4741 ctcgatgatc gagcgctcgt cggcccggat cgccggatcg gtgatgatcc gatccaactc
     4801 ctgccggtag acggcgactt cgtccggggt gatgagctcc tcgacggcga ggaagccatc
     4861 gcgctcgtac gcctggaggt cggtgaggga ggccgggccg ggcgtgccgg gggctcccca
     4921 gacgaccggg tcctgacggg ggaccgtcac ctcggaggcg ccgcggctgg ggtacaggtc
     4981 ggtgatcgtg gtcatggtgg tcagccctcc tcggtgagca gtgggtagac gccgttctcg
     5041 tcgtggtcct cccggccggt cacgggcggg ttgaacacgc agacgcagcg gaagtcctcc
     5101 ttgacccgca gcgtgtgccg ctcgtgcccg tcgaggaggt acatggtccc gggcgtgatc
     5161 gtgtacttcc gcccggtctc gtcgtcggtg agctcagcct cgccctcgac gcagacgacg
     5221 gcctcgatgt ggttcgcgta ccacatcgac gtctccgtgc cggcgtagag gatcgtctcg
     5281 tgcagggaga agcccacctt ctccttggcg aggacgatgc gtttgctctc ccaggtgccg
     5341 gacgccgatt tcacgtgccg gtcggtgcct tcgatgtcct tgaacgaacg gacgatcacg
     5401 gtgggttcaa gcctccttct gtagccttct cggacttctc agccctctcg gacttctcag
     5461 ccttctcgga cggtttcgcg gacggcgcgg gccagcgtgc gcaggccctc gtccagttcg
     5521 tcgggggtga tggtgagggc gggcagcagt ttgacgacct cgccctccgg gccggacgtc
     5581 tcgatcagca gcccgagtgc gaaggcccgc cgagccaccc gctccgcgcg cgccttgtcg
     5641 tggaactcga tgccccacac cagcccgcgc ccccggtact ccttcacctc ggcgaggttc
     5701 tcctcggtga tcgagatcag cgcctgctcg atctgctcac cgcgcgcccg ggtctgcttc
     5761 tccatggcgg agccgtccga ccagtacgtc tccagcgcgg cggtggcggt gacgaaggcc
     5821 gggttgttgc cgcggaacgt gccgttgtgc tcgcccggct cccagatgtc cagctcgggc
     5881 ttgaacaggc acagcgacat gggcagcccg tacccgctga tggacttgga gacggtgacg
     5941 atgtcggggg tgatgcccgc ctcctcgaac gagaagaagg cccccgtacg cccgcagccc
     6001 atctggatgt cgtcgacgat cagcagcatg tcctggcgct cgcacaggtc ggcgagcgcg
     6061 cgcagccact ccgggcgggc gacgttgatg ccgccctcgc cctggacggt ctccacgatc
     6121 acggcggccg gcttgttgag cccggagccc tggtcctcca ggagccgctc gaaccacagg
     6181 aagtcctcga ccgtgccgtc gaagtagttg tcgaacggca tgggcgtgcc gtgcacgagc
     6241 gggataccgg ccccggcccg cttgaaggcg ttgccggtga cggcgaggga cccgagggac
     6301 atgccgtgga aggcgttggt gaaggagacg atcgcctccc gccccttcac cttccgggcc
     6361 agcttcagcg cggactcgac cgcgttggtg ccggtcggcc ccgggaacat gaccttgtac
     6421 ggcagatcgc gcggccgcag caccaggttc tggaacgact ccaggaaggc ccgcttggcg
     6481 gtcgtcgaca tgtcgagccc gtgggtgacg ccgtcccgct ccagatagtc gatgagggcg
     6541 cgtttgagta cggggttgtt gtggccgtag ttgagtgaac cggcgccggc gaagaagtcc
     6601 agatacgtgt ggccgtcctc gtcgtacata cggctgccgt gcgcgcggtc gaagacggtg
     6661 ggccagccgc ggcagtagct gcgcacctcg gactccaggg tctcgaagac gctcaggtcc
     6721 ggctgggtga tggtcacggt cgatcgctcc tcgatgcgtg ggggggtgag agctcttcgg
     6781 gggtcagatt ccgcggggtt tggactccgc gggttcagac ggcgagcggg ccgatgcggt
     6841 ggaggacctc cggctggtgg gagccgtccg ggaagtcccc cgcctcgaac aggacggtgc
     6901 gctcgatgcc cgcgccgtgc cgttcggcgt acgcggcgaa caggcgctcg gacgcggtgt
     6961 tgcccggcgt gatggtggtc tcgacggtcg tcagggtctg ttggcgggcg acccgggcgg
     7021 tgagcccgtc cagcagcgcg gcggcgagtc cgcgcccccg gtgcgcctcg tcgacggcca
     7081 cctgccacac gaggagggtg tccggccgct ccggccgcac gtacccggtc acgaacccca
     7141 ccggttcccc cgactcgtcc cgcgccaccg ccgaggtggc ggcgaagtcc cggcaccaca
     7201 gcagatagct gtacgaggag ttcagatcga gggtcccgga gtccccggcg atacgccaca
     7261 gcgccgcgcc gtccgtaacg tccgggcggt cgatccgaat cccctccggg attttcagtt
     7321 ctgcttgtgc ggcttctgct tgtgcggcag tcatgtggat tgaatttacc gaggggaaat
     7381 tcgaatcgca ttgctgcggg gggttacgca tgggctgccc gtgtgctatc aggcgggcgc
     7441 gagcgagcgc gcgagaaggc ggtcatgatg tgtgcatttg cccggaaaat accgcacaaa
     7501 aagggcgcga tgtggagtcg gtcacatgtg tgcaaccctc acgagatctg ctcgaattgt
     7561 gtccgatcca cgcccatctg tgttcgcgaa atctttgcgt ttaggagagg gaagaacggg
     7621 caggagaatg cggggccctg ttttgattaa tccgctggaa ttccagttgg aaagcctcgc
     7681 cgagaagtcg gggaaaccgg aggaatgcga gggagtggtc agaacccggt gaccccgtcg
     7741 atgcgctcgc gcacgatgtc ggcatggccg ttgtgccggg cgtactcctc gatcatgtgg
     7801 acaaggatcc accggaggct gaccgccagc ccgcccatca tctggccgtc cgggatccgc
     7861 ccgagcgagt cgagagcgcg tcccgccacc gtcgcgcgac tgcgggcgac ctcccgccgc
     7921 cagatgtaga gcgcctcgtc gagcccgcgt tcgggggaca gccggtaacc cgtgccctcc
     7981 tcgtacacca ggggagcgtc cacgcccgcg aacacccgct ggaaccagtt ccgttcgacc
     8041 tccgtcagat gctgtacgag ccccagcagc gtgagttccg acggctccgc cgacgccgtc
     8101 cgcgcctgcg cgtcatccag ccccgcacac ttcaactcca aagtggcccg atggaactcc
     8161 agccaactct ccagcatggg gccctcgtcg ccggtcatca ggggaatggg tcggccgtcg
     8221 gggagagtgg cgggtacggg tacgggtgcc gactcggggg tggtcccggt gctcctgggc
     8281 tcggtcatgg catcggtggg ctcggaagtg gtcatgagcc caccctcaca cgcgcccccg
     8341 gcctctaccc ccaggtctcc gaagccgccc ggcgagcccc gtccgggtcg accgtcagcc
     8401 ccgcctcggc catcgccgcc cccagcccgg ccaggctgcc gtgcaccgcc tcgggggagg
     8461 cgtccgggcc gtagtggttg acccggacca tctccttggc gagggcgccg ccgcccgcgg
     8521 ccagcggccg tgcggggtcc acggccagcg ccctggcgac cagttcggag gcgtcgaccc
     8581 cggcgggcac ccggagggtc gtggcgacgg gcgccgcgtc ccgcgcctcg tacacatacg
     8641 gctccaggcc gccgcccagc gctagcgcac ccgcccgggt cgcggcggcg gcggatgcgt
     8701 gccgggacat gaccgtgtcc ggtccggccg cctcgatgcg ctccaggcag gcctccaggg
     8761 cgagcatctc caactgggcg ggcgcgtgga gcagtgcacg ccggccgccg tcgatccagc
     8821 gctccttcca gtcgaggagc gagaggtacg accgccgcgg ggcccgcggg ttcgcggcca
     8881 tccgggccca cgcccgctcg ctcacggaga tcgccgacac cccggccggc ccgcccatgg
     8941 ccttctgcgc gccgatgacg cacaggtcga caccccaggc gtcgggcagc accggctcgg
     9001 cgccgacgga ggcgacggcg tcgaggtaga acagggcgcc gtgctcgcgt acgacctcgc
     9061 cgatctccgc gacggggttg tgaacaggtg gtgattgacg gcagttgagt gtgttctggc
     9121 gcgtatgagt gagtctccgg gcgtggcagg cgcacatccc cccgggtggg gaacgggagt
     9181 tcttgtgtcg aatgtgtgac ctttgccggt ggtggtcgtt gggtgtggtg acggatttct
     9241 gcgacaccgg ctggggggtg gaagagatgg cgtcctgcgt gagggctgaa cgtcctgcgt
     9301 gaggactggg cgtcctgcgc gaggactggg cgtcctgcgc gagggctgag ctcccggcgt
     9361 tactcctcgc cgtccttgtt gttcttcttc tccctgttcg cccgcttctc gtcctcctgc
     9421 cgcttcaact cctcctcgcg gcgacggaga tcggcctccc agctcttgag gagggactcg
     9481 tccttcttgt tgtcctcggc gagggacttc aggaactcgg ggttgtcgtc cggggccacg
     9541 tactcgaggc ggtggtggcg gtgccactcg gagggggtgc ggccgttcgc gggggcgtgg
     9601 cgcatcttgc ccgccgccag ccaggcgatc gggccgacga tccagaagag caggatgatg
     9661 aagacccagg cgatcttggg aaggtgcttc gcctcgtcct cgggggtgtt gaggcagtcg
     9721 atgaacgcgt agatcgtcag cgccaacggc acgaggtaca tcaacaccct gagcatgaac
     9781 ggcccccctg gaagcggtga cggcggtgcg ggagccgtac atgtcggccc tgcacccatg
     9841 atgacgggac cgcgcgccgg ggccccgtga cggggtcagg gtagcccctc ggggatactt
     9901 gaccccatgg cttacgacga tcttcgctcc ctgctccgcg cactggagcg cgagggcgat
     9961 ctcaagcgca tcaaggccga ggtcgacccg tatctggaag tcggggagat cgtcgaccgg
    10021 gtgaacaagg ccggaggtcc ggcgctgctc ttcgagaacg tgaagggctc ctcgatgccc
    10081 ctcgcgatga acgtcttcgg caccgaccgc cgcctgctga aggcgctcgg cctgaagtcg
    10141 tacgaggaga tcagcggcaa gatcggcggc ctgctcaagc ccgagctgcc gcacgggttc
    10201 gtgggggtgc gcgaggcctt cgggaagctc ggcgcgatgg cccatgtccc gccgaagaag
    10261 gtgaaatccg acaacgcgcc ggttcaggag gtcgtcctgc tcggcgacga cgtggacctg
    10321 gagcggctcc cggcgctgtt cacctggccc aaggatggcg gctccttctt caatctgggc
    10381 ctcacccaca ccaagcacc
//
