LOCUS       NZ_CP022161            10207 bp    DNA     linear   CON 15-DEC-2024
DEFINITION  Streptomyces capitiformicae strain 1H-SSA4 chromosome.
ACCESSION   NZ_CP022161
VERSION     NZ_CP022161.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces capitiformicae
  ORGANISM  Streptomyces capitiformicae
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10989391)
  AUTHORS   Liu,C.
  TITLE     Streptomyces capitiformicae sp. nov., a novel actinomycete producing
            naphthoquinone compounds isolated from the head of Camponotus
            japonicus Mayr
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10989391)
  AUTHORS   Liu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUN-2017) Life of Science, Northeast Agricultural
            University, No. 59 Mucai Street, Harbin 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. Feb-2017
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002214185.1-RS_2024_12_13
            Annotation Date                   :: 12/13/2024 14:26:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,013
            CDSs (total)                      :: 9,935
            Genes (coding)                    :: 9,274
            CDSs (with protein)               :: 9,274
            Genes (RNA)                       :: 78
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 661
            CDSs (without protein)            :: 661
            Pseudo Genes (ambiguous residues) :: 0 of 661
            Pseudo Genes (frameshifted)       :: 220 of 661
            Pseudo Genes (incomplete)         :: 571 of 661
            Pseudo Genes (internal stop)      :: 32 of 661
            Pseudo Genes (multiple problems)  :: 155 of 661
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:06:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3380057
            Orig. end                         :: 3390264
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP022161.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10207
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="13"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10207
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10207
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5207](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5207
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            <575..874
                     /locus_tag="CES90_RS14975"
                     /pseudo=""
     CDS             <575..874
                     /NRPS_PKS="Domain: Polyketide_cyc2 (18-95). E-value:
                     6.5e-07. Score: 21.8. Matches aSDomain:
                     nrpspksdomains_CES90_RS14975_Polyketide_cyc2.1"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017946638.1"
                     /locus_tag="CES90_RS14975"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="VTGEVTRTAYFLGRRIDYVLRVAAYDPPRLLDMISVAGPMPMHVT
                     YAFEPHPRGTLARIRVRGGGGGFYRLAAPLLGRQVRANLAKDLRDLEARLMAQG"
     aSDomain        632..862
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_CES90_RS14975_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="6.50E-07"
                     /label="CES90_RS14975_Polyketide_cyc2.1"
                     /locus_tag="CES90_RS14975"
                     /protein_end="95"
                     /protein_start="18"
                     /score="21.8"
                     /tool="antismash"
                     /translation="VLRVAAYDPPRLLDMISVAGPMPMHVTYAFEPHPRGTLARIRVRG
                     GGGGFYRLAAPLLGRQVRANLAKDLRDLEARL"
     gene            complement(899..1267)
                     /locus_tag="CES90_RS14980"
     CDS             complement(899..1267)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1058: ArsR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656187.1"
                     /locus_tag="CES90_RS14980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_189785439.1"
                     /transl_table=11
                     /translation="MSTSTPLYQLKAEFFKTLGHPARIRVLELLSEREHAVAEMLPEVG
                     IEPAHLSQQLAVLRRANLVVTRKEGSTVYYSLTSPHVAELLRVARTILSGVLAGQAELL
                     ADLRAAQGEAQGEPRPPS"
     gene            complement(1480..1923)
                     /locus_tag="CES90_RS14985"
     CDS             complement(1480..1923)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005473382.1"
                     /locus_tag="CES90_RS14985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding protein"
                     /protein_id="WP_189785438.1"
                     /transl_table=11
                     /translation="MSTQLEALPYRHVVTLPTEPSSVRMARETAEQALVEWGMGLRHPA
                     VDPALLILSELVTNSVRHAAVLSPQVTVIYAAGRDCLAFAVHDRHPYQPPLHDAATGTG
                     AGGPAGSGGLSTVMELVLGLGGTAVVRADADGRGKSIWITLPL"
     gene            complement(1923..2330)
                     /locus_tag="CES90_RS14990"
     CDS             complement(1923..2330)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656190.1"
                     /locus_tag="CES90_RS14990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="pyridoxamine 5'-phosphate oxidase family protein"
                     /protein_id="WP_189785437.1"
                     /transl_table=11
                     /translation="MSTSPVPLRMVEVSGAEALWLLEGNTLGRLVYMQRDLTVVRPARH
                     VWEYGRLIVRTPVPAPAVPETATYHADEIRAVHGTGWTVTVSGPAEVITDPDEAAHYRR
                     TLSGWAHGPHDTLLCLHPKTVTGFRLARAEV"
     gene            complement(2327..4069)
                     /locus_tag="CES90_RS14995"
     CDS             complement(2327..4069)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670130.1"
                     /locus_tag="CES90_RS14995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_189785436.1"
                     /transl_table=11
                     /translation="MLVMLGRVGARIAALLPVRADLTEIRRDPRRDLLAGLAVAIVALP
                     LALGFGVSSGLGAEAGLATAVVAGALAALFGGSNLQVSGPTGAMTVVLVPIVGEYGPTG
                     VLTVGLMAGVMLVALAALKAGRYMQYVPAPVVEGFTLGIACVIGLQQIPNALGVPKPDG
                     DRVLVVTWRAIEEFTKSPNWTALGFAVAVAAVMLVGARWRPAVPFSILAVIAATVVARA
                     AGLDAAEPIGDLPSGLPAPSLSFLDVGSLGTLMAPAVAVAALAALESLLSASVADGMTV
                     GQKHDPDRELFGQGLANIAAPLFGGVPATGAIARTAVNVRTGAGSRLAALTHAALLAVI
                     VFAAAPLVSWIPLAALAGVLLATAIRMVEVGSLRAMAKATRSDALILVLTAVATLALDL
                     VYAVIIGLAVAGALALRAVAKQARFDQVPLDRGDHSAEEHALLAEHIVAYRIDGPLFFA
                     AAHRFLLELTDIADVRVVILRMSRVSTMDATGALVLKDAVEKLRRRGIVVLASGIRPGQ
                     RQVLDSVGALDLLRYEDPEGGQGREYATTPEAIRAARRHLEKAGVLPALPAQKPRTSSE
                     GALR"
     gene            complement(4263..4628)
                     /locus_tag="CES90_RS15000"
     CDS             complement(4263..4628)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019070741.1"
                     /locus_tag="CES90_RS15000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="WP_189785435.1"
                     /transl_table=11
                     /translation="MNENPPPCPKCSCEYTYEMNALVVCPECSHEWVPAESSETGVPPA
                     EGWGKVVKDSVGNVLRDGDSVVVVKALKVKGSPSGIKAGTKVRGIRLVDGVDGHDIDCK
                     IDGFGAMQLKSGVVKKA"
     misc_feature    4593..4607
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.88"
                     /tool="antismash"
     misc_feature    complement(4610..4625)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.82"
                     /tool="antismash"
     gene            5001..5207
                     /locus_tag="CES90_RS15005"
     CDS             5001..5207
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311933.1"
                     /locus_tag="CES90_RS15005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_189785434.1"
                     /sec_met_domain="strepbact (E-value: 1.6e-14, bitscore:
                     53.3, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSAPQIETAEISDAALDNISGGLNPHVSVVAGPATITDADVLAQ
                     VDAVKDQVLGAVGHHQVGVCVSI"
     gene            complement(5414..6610)
                     /locus_tag="CES90_RS15010"
     CDS             complement(5414..6610)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539000.1"
                     /locus_tag="CES90_RS15010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_189785433.1"
                     /sec_met_domain="DAO (E-value: 8e-08, bitscore: 31.4,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGHILVIGGGTAGTATALALHKAGFDVTVYEAHPDAAEDIGAFLT
                     LASNGMRALAQIDASAAVTALGFPLTGMRVLDGSGAEVASRPLGEAEDPVLRYRCMRRG
                     ELNAALQAEVSRRGITIRHGVRLASVQGGPESVTAHFADGSTATGDLLVGADGLHSTVR
                     RSIAPDARPCYAGEYVFYGYTTDASLPEDTERITFSRSSSAAFGHAVSPAGEAYWFARV
                     AGEPLSPEEIAAGTPTRWRDLLVPLLREDATPAAGIVAATAGDILVTNATEMPPAMPWR
                     SGRSLLIGDAAHAASPATGQGASMALEDAVVLAKALRDAPDTETALSLYETLRRPRVEH
                     NITVSGQISRGAAAPTRPTSPPPRPGDEAALIRLLEWDADISTTDISTTDISTPPAGS"
     gene            complement(6750..7010)
                     /locus_tag="CES90_RS15015"
     CDS             complement(6750..7010)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009716652.1"
                     /locus_tag="CES90_RS15015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_229914099.1"
                     /transl_table=11
                     /translation="MAKQFKKSDNSSGQGLLWLFGAILVVQGFGSAITEAGWDTSFGVA
                     GLLRAAHVPEWGTLLVGFAGAALLGIAARRHHAKRQGHRRP"
     misc_feature    complement(6807..6809)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(7042..7060)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.74"
                     /tool="antismash"
     gene            7120..7716
                     /locus_tag="CES90_RS15020"
     CDS             7120..7716
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040295.1"
                     /locus_tag="CES90_RS15020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_189785432.1"
                     /transl_table=11
                     /translation="MPRQVDYEDRRRRIAEAVCTLIARSGMEAVSLRDVAAQADVSMGA
                     VQRCFRTKEDMLLFALEHVSQRVSERANERIATASTPQAASTLLSHTLAELALLDEESK
                     TQAHVWLAFITHAPASERLAAVLHDSYAKLHDLIAWLIRYGQSSGEVPQHLDPIREAHT
                     LLAVADGLTVHVLAGHHSPETALTILQRHIDHLLQ"
     gene            complement(7838..8146)
                     /locus_tag="CES90_RS15025"
     CDS             complement(7838..8146)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010350112.1"
                     /locus_tag="CES90_RS15025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_189785431.1"
                     /transl_table=11
                     /translation="MMTGLRLLPWTTPDGKPCYLDPDTAYPGPLSQLADSVEVMQLGSA
                     EDVLVGGRAVLADSAAGERALRFALTRAAESLEDVLRIAVSRGRRIEAAGEDGDKEP"
     gene            complement(8143..8370)
                     /locus_tag="CES90_RS15030"
     CDS             complement(8143..8370)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CES90_RS15030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_229914098.1"
                     /transl_table=11
                     /translation="MALRRTAPRTWSTPGPRPAQRTGDSQPLDPGPPPPLPRKPVDDLW
                     PFEPYDNFVRPYVLTAEELHVAYGMEGVPA"
     gene            8537..8662
                     /locus_tag="CES90_RS51615"
     CDS             8537..8662
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CES90_RS51615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_308437887.1"
                     /transl_table=11
                     /translation="MSERVDMDAESGRAVLGRTLRLLREKAGKSLGQLADETGYD"
     gene            8714..9361
                     /locus_tag="CES90_RS15035"
     CDS             8714..9361
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040183.4"
                     /locus_tag="CES90_RS15035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5753 domain-containing protein"
                     /protein_id="WP_308437886.1"
                     /transl_table=11
                     /translation="MKDLDVYYGAGDLLVSHWKVARLDAFKDQYKRFMELEATARIMWK
                     FTLGIPGLMQIEDFARGVLSGAQTTADVDEAVEEQVAARLGRQYLLRQKPEPDLRIIMD
                     EFALRRPAAGGKTWHDQLLHLETAARWPNLTLQVLPFSAGAHHHMNGSLTLLWQRDGSA
                     VAYKEGNGCSRLIEDSDEVLRQRLSYDRLRDLALSPPDSLALIKDALKEHRS"
     gene            9358..9561
                     /locus_tag="CES90_RS15040"
     CDS             9358..9561
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056019.1"
                     /locus_tag="CES90_RS15040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_189785430.1"
                     /transl_table=11
                     /translation="MTRTLDLSTAVWRKSSYSEGGDNDCVEVADGQPGIVPVRDSKVPD
                     GRALLVSAASWSAFVRSVKAAP"
     gene            9558..9755
                     /locus_tag="CES90_RS15045"
     CDS             9558..9755
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515816.1"
                     /locus_tag="CES90_RS15045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_189785429.1"
                     /transl_table=11
                     /translation="MTPDLTTAVWRKSSYSDGGANNCLEVADGYIGTVPVRDSKRPDAH
                     PLLLSAASWSAFVRSVKAAP"
     gene            9752..9961
                     /locus_tag="CES90_RS15050"
     CDS             9752..9961
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115735.1"
                     /locus_tag="CES90_RS15050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_189785428.1"
                     /transl_table=11
                     /translation="MTPDLTTAVWRKSSYRDGGGTNCLEVADGHPGIVPVRDSKVPDGR
                     PLLVSAAAWSAFVGGYAPGAGQGR"
ORIGIN
        1 tcttcgagcc gctgcccggc gagggccgct gggacaccgt cctgctcatg gacggcaaca
       61 tcggcatcgg cggcgacccg cgcgccctgc tggaccgggt ggccgggctc ctgcgccccg
      121 gcggcctgct gatcgccgag accgccccgg tggacgtcga cgaacgcgta cgcgtccaca
      181 tcaccgatgc ccacgccacc gtcagtgccc ccttcacctg ggcgcggctc ggcacccgcg
      241 cgctgctccg gtacgcgcgg ggctgggagc gggccggtca gtggacgtcc ggcggacggc
      301 gcttcgccgc cctgcgcagt cccggcagcc gcagcgccgg cagcagcgcc gagccgccga
      361 agaggacggc ggtgatcagc agccagaggg cgaggaacac gtccgccggc aggccggtgg
      421 cggactcgta gcgccgctcc accctgccgc cgatcagcgg gaaccacacg agaaggagca
      481 gcccggacaa ggccgccggc acgcgcacgt acggcacccg gttccggcgc cggaccgcgc
      541 cgagtcctcg tacgaccgcc cggtccagcg ccgtgtaacc ggcgaggtca cgcgtacggc
      601 gtacttcctg ggccggcgca tcgactacgt gctgcgggtc gccgcgtacg acccgccccg
      661 gctgctggac atgatctccg tggcgggccc gatgcccatg cacgtcacct acgccttcga
      721 gccccacccg cgcggcacgc tcgcccgcat ccgggtgcgg ggcggcggag gaggcttcta
      781 ccggctggcg gcaccgctgc tggggcgaca ggtccgcgcc aacctcgcca aggacctgcg
      841 cgacctcgaa gcgagactca tggcacaggg gtgagacccc ggcggcgtca caccctcgct
      901 acgacggcgg cctcggctcc ccctgtgcct ctccctgcgc ggcccgcagg tcggcgagga
      961 gttcggcctg gccggccagt acaccggaca ggatcgtccg tgccacccgc agcagctcgg
     1021 cgacgtgcgg gctggtgagg gagtagtaga cggtcgagcc ctccttgcgc gtcaccacca
     1081 ggttcgcgcg ccggagcacg gcgagttgct gggagaggtg cgcgggttcg atgcccacct
     1141 cgggcagcat ctcggcgacc gcgtgctcgc gctcgctcag cagctccagg acccggatgc
     1201 gtgccgggtg gccgagcgtc ttgaagaact cggccttcag ttggtacagc ggcgtactcg
     1261 tactcatcgt gccgtcgcct ccccgaccca acacctgcac caacagcgcg gccctaccgg
     1321 ccgggccccg gcgcatcgca tccatccact cgtcgacaac atgcggccca tcctcgcctg
     1381 cggcggtcat ggtcgtgaac ttccctgtgt gtgccgcccc ttgggtgaca ggggcaggtg
     1441 gactcaaagg ggcaactcgg tttgaagggt caggcgggtt caaaggggca gggtgatcca
     1501 gatgctcttg ccgcgtccgt cggcgtccgc gcggacgacc gcggtgccgc ccaggccgag
     1561 gaccagctcc atgacggtgg acagaccgcc cgacccggcc ggtccgcccg ccccggtgcc
     1621 ggtcgccgcg tcgtgcagcg gaggctggta ggggtggcgg tcgtggacgg cgaaggccag
     1681 gcagtcccgg ccggccgcgt agatcacggt cacctgcggg gagagtacgg cggcgtgccg
     1741 gacgctgttg gtgaccagct cgctcaggat cagcagggcc gggtccaccg cgggatggcg
     1801 cagccccatc ccccactcga ccagggcctg ctcggccgtc tcgcgggcca tgcgtacgga
     1861 ggacggttcg gtcggcaacg tgaccacgtg ccgatagggc agggcttcca gctgcgtgga
     1921 catcacacct ccgcgcgggc gaggcggaac ccggtgaccg tcttggggtg caggcacagc
     1981 agggtgtcgt gcgggccgtg ggcccagccg gagagggtgc gccggtagtg cgcggcctcg
     2041 tccgggtcgg tgatcacctc ggccggtccg gagacggtga ccgtccagcc ggtgccgtgc
     2101 acggcacgga tctcgtccgc gtggtacgtc gccgtctcgg gcaccgccgg cgccgggacc
     2161 ggcgtgcgca cgatcagccg cccgtactcc cacacatgcc gggcgggccg tacgacggtc
     2221 aggtcgcgct gcatgtacac cagccgtccc agggtgttgc cctccagcag ccacagggcc
     2281 tccgcgccgg agacctcgac catacggagg gggacggggg acgtgctcat cgcagggctc
     2341 cctcgctgga ggtacggggc ttctgtgcgg gaagggcggg caggactccg gccttctcca
     2401 ggtgacgacg ggcggcgcgg atcgcctcgg gggtggtggc gtactcccgg ccctgtccac
     2461 cctcggggtc ctcgtaccgc agcaggtcca gcgcgccgac cgagtcgagg acctggcgct
     2521 ggccgggacg gatcccggag gcgaggacga cgatgccacg acgccgcagc ttctccacgg
     2581 cgtccttgag gacgagggcg ccggtggcgt ccatcgtcga cacccgggac atacgcagga
     2641 tcacgacccg tacgtcggcg atgtcggtca gctccagcag gaagcggtgc gcggcagcga
     2701 agaacagcgg cccgtcgatg cggtaggcca cgatgtgctc ggcgagcagc gcgtgctcct
     2761 cggcactgtg gtcgccgcgg tcgaggggga cctggtcgaa gcgggcctgt ttggccacgg
     2821 cacgcagggc cagggcgccc gcgacggcga ggccgatgat gacggcgtag acgaggtcca
     2881 gggccagggt cgcgaccgcg gtcaggacga ggatcagcgc gtcggaccgg gtggccttcg
     2941 ccatggcccg cagcgagccg acctcgacca tgcggatcgc ggtggcgagc aggactccgg
     3001 cgagtgcggc aagggggatc cacgagacca ggggggccgc cgcgaagacg atcacggcga
     3061 ggagcgcggc atgggtcagg gcggcgagcc gggaaccggc gcccgtacgg acgttgaccg
     3121 cggtccgggc gatcgcgccg gtcgcgggga cgccaccgaa gagcggggcg gcgatgttgg
     3181 ccaggccctg gccgaacagc tcccggtcgg ggtcgtgttt ctggccgacc gtcatgccgt
     3241 cggcgaccga ggcggacaac agggactcca gggcggccag ggcggcgacc gccacggccg
     3301 gggccatgag cgtccccagg gagccgacgt ccaggaagga caacgaaggc gcgggcagac
     3361 cggacggcag atcgccgatc ggttcggcgg cgtccagccc ggcggcccgc gcgaccaccg
     3421 tcgcggcgat cacggccagg atggagaacg gaacggccgg ccgccaccgc gccccgacca
     3481 gcatgaccgc cgcgacggcg acggcgaagc cgagagcggt ccagttcggg gacttcgtga
     3541 actcctcgat cgcccgccag gtcaccacca gcacccggtc gccgtccggc ttgggcacac
     3601 cgagggcgtt cgggatctgc tgcaggccga tcacgcaggc gatgccaagg gtgaagccct
     3661 cgaccacggg ggcgggaacg tactgcatgt aacggccggc cttcagcgcg gccagcgcca
     3721 ccagcatcac cccggccatc agaccgaccg tgaggacacc cgtcggcccg tactcgccca
     3781 cgatggggac gagcaccacc gtcatggcgc cggtcggccc cgacacctgc agattcgatc
     3841 ccccgaagag cgcggcgagc gcaccggcga ccacggcggt cgccaggccc gcctccgcgc
     3901 cgaggccgga ggagactccg aagccgaggg cgagcggcag cgccacgatc gccacggcca
     3961 gcccggccag caggtcccgg cgcgggtcgc ggcgtatctc tgtgaggtcg gcgcggacgg
     4021 gcagcagcgc cgcgatccgg gccccgaccc ggcccaacat aaccaacata tccagcacag
     4081 catctaacca attgcgaaaa tttgcaattc agcagcagcc cactgcggcg gtgtcttccg
     4141 tcacggacgg gtcgaccggg cgagccggct ggcggagcgg gtcgaggggc ggagcgggtc
     4201 ggggggcgga gcgggccggg gggcggagcg ggccgggggg cggagcgggc cggggggcgg
     4261 gatcaggcct tcttgaccac gccggacttc agctgcatcg ccccgaagcc gtcgatcttg
     4321 cagtcgatgt catggccgtc aacaccgtcg accagccgga tgccgcgcac cttggtgccg
     4381 gccttgatcc ccgaggggct gcccttgacc ttgagcgcct tgacgacgac cacgctgtcg
     4441 ccgtcccgca gcacgttgcc cacggagtcc ttgacgacct tcccccagcc ttcggccggg
     4501 gggacccccg tctcgctgct ctcggcgggc acccactcgt gactgcactc cgggcacacc
     4561 accagggcgt tcatctcgta ggtgtactca caggagcatt tcggacaggg cggaggattc
     4621 tcgttcatcc ggtcagcgta tccggcggaa cccactccgg cttccgcgaa cctgcacatc
     4681 gattgcgcaa ccgcggcaca gggtgcggcg gatctgcaca acggtccgcc cgatagcgtc
     4741 ctgttggcac cgagtgcccg cttcggcatc gtgacggcaa agcccgtgac ctcccggcgc
     4801 cgcgcggata tatggagaag ccgtcctcaa gccgtgcgca cgccgacacg gaacagtcgc
     4861 ccgtgcgttc aacggccctg gtgggagcgt tggttgagtg gatgccccgg cgagctccgt
     4921 gctttgatcc tctgcacccg ccctctgtcc ccagcggggc cacccccctt gtggatcacc
     4981 atacgaaggg aagtctcctc atgaactccg ctccccagat cgagaccgcc gagatctccg
     5041 acgccgcgct cgacaacatc tccggcggcc tgaacccgca cgtcagcgtc gtcgccggcc
     5101 cggcgacgat caccgacgcg gatgtcctgg cccaggtcga cgcggtgaag gaccaggtcc
     5161 tgggcgccgt cggccaccac caggtcggcg tctgcgtctc catctgaccc aggttccgac
     5221 ccgggctgca gggaacggcg gagctcctgg cggcaacggg gtctcaagaa gagatcccgg
     5281 cccgccagga gcttcgtgca tgttcgggtt ctgcttcaga cgggccgtga agtgaggatc
     5341 acggcggcgc cggcccccgc acccgccaga cccgcctgac cgcacaacaa cggcagcagg
     5401 agaccgtacg gcgtcatgac cccgccggtg gggtgctgat gtcggtggtg ctgatgtcgg
     5461 tggtgctgat gtcggcgtcc cactccagga gccggatcag ggcggcctcg tcgccgggcc
     5521 gcggcggggg cgacgtcggg cgggtggggg cagcggcgcc tcgggagatc tggccgctga
     5581 cggtgatgtt gtgttccaca cgggggcggc ggagggtctc gtagagggag agcgcggtct
     5641 cggtgtccgg ggcgtcccgg agggccttcg cgaggacgac ggcgtcctcc agcgccatgg
     5701 aggccccttg cccggtggcc ggcgaggccg cgtgcgccgc gtcgccgatg agcagggacc
     5761 gtccggagcg ccacggcata gccggtggca tctcggtggc gttggtgacc aggatgtcgc
     5821 cggccgtcgc cgcgacgatg ccggcggcag gggtggcgtc ctcacgcagc aacgggacga
     5881 gcaggtcgcg ccaccgggtc ggcgtgccgg ccgcgatctc ctcgggcgac agcggctcac
     5941 ccgcgacgcg ggcgaaccag tacgcctcgc ccgcgggaga caccgcatgg ccgaaggcgg
     6001 ccgagctgct gcggctgaac gtgatgcgct cggtgtcttc gggcagggac gcgtcggtgg
     6061 tgtagccgta gaagacgtac tcccctgcgt agcagggccg cgcgtcgggg gcgatcgacc
     6121 ggcggacggt ggagtgcagc ccgtcggcac cgaccagcag atcaccggtc gccgtactgc
     6181 cgtcggcgaa gtgcgcggtg acggactcgg ggccgccctg tacggaggcg aggcggacgc
     6241 cgtgcctgat ggtgatgcct ctgcgtgaca cctccgcctg cagtgcggcg ttgagctcgc
     6301 cgcgacgcat gcaccgatac cgcaggaccg ggtcctcggc ctccccgagc ggcctgctcg
     6361 ccacctcggc cccggagccg tcgagcaccc gcatgccggt cagcgggaag ccgagggcgg
     6421 tgaccgcggc ggaggcgtcg atctgcgcga gagcacgcat cccgttgctc gccagcgtga
     6481 ggaacgcccc gatgtcctcg gccgcgtcgg gatgcgcctc gtacacggtg acgtcgaacc
     6541 cggccttgtg cagcgcgagc gccgtcgccg taccggctgt cccgcccccg atgacgagta
     6601 tgtgccccac gcctgctccc ccgccgagtc cgcccggccc ggtcagccgt gctcgtcatg
     6661 ggtcgaactc ccgggcggtg tccgggagtt ccccggcgga ccccgctcag ctcgtgggcc
     6721 ggcggccggc agctccgaca aaccactgct caaggacgtc ggtgcccctg cctcttcgca
     6781 tggtgacggc gcgccgcgat ccccagtaag gcggcacctg caaagccgac cagaagggtc
     6841 ccccactcag gcacgtgggc tgcccgcaga aggccggcga caccgaagct tgtgtcccac
     6901 ccggcctccg tgatggcgga gccgaagccc tggacgacga ggatggcacc gaacaaccag
     6961 agcagcccct ggcctgagct gttgtccgat ttcttgaact gtttcgccac gattggaggt
     7021 cccttcagtt gtacacacat cgttccgatg cgatcgcatc acatcgtgca gcgtagccgg
     7081 atatgatgca agtgcatcgg aaaacaggag gagcgaggcg tgcccagaca ggtcgactac
     7141 gaggaccgac gccgccggat cgccgaggcg gtctgcactc tgatcgcgcg ttccggcatg
     7201 gaggccgtca gcctccgcga cgtcgccgct caggcggacg tgtccatggg tgccgtccag
     7261 cggtgcttcc gcaccaagga ggacatgctg ctcttcgcgc tggagcacgt ctcccaacgc
     7321 gtcagcgaac gcgccaacga gcgcatcgcc acggcctcca cccctcaggc cgcctccact
     7381 ctgctgtccc acaccctggc cgaactcgcc ttgctggacg aggaatcgaa gacgcaggca
     7441 cacgtctggc tggccttcat cacccacgcc ccggccagcg agagactggc cgccgttctt
     7501 cacgacagct atgccaagct ccacgacctc atcgcgtggc tcatccgcta tggccagagc
     7561 tccggggaag tcccacagca cctcgacccc atccgcgagg cgcacaccct cctcgccgtg
     7621 gccgacggcc tcacggtcca cgtcctcgcg ggccaccact ccccggagac ggcactgacc
     7681 atcctccagc gccacatcga ccacctgctg caatgagctc cagccagggg ttgggcgggg
     7741 tgctattggc agttccgtct cggcgacacg ccgcgtttgc gacgtgaggg ccgggacagc
     7801 cgagacaaat ctgccaatcg tcgagcacct gcgactgtca gggttccttg tcgccgtcct
     7861 caccggctgc ctcgatccgc cgccctctgc tgaccgcgat ccgcagcacg tcctccagcg
     7921 actccgccgc ccgcgtgagc gcgaaccgca acgcccgctc gccggccgcc gagtccgcga
     7981 gtacggctct gccgccgacc aacacgtcct ccgccgaccc gagttgcatg acctccacgc
     8041 tgtcggcgag ctgggagagg gggccggggt acgcggtgtc ggggtcgagg tagcagggct
     8101 tgccgtccgg ggtggtccag ggcagcagcc gcaggccggt catcatgcgg ggactccttc
     8161 catgccgtac gccacgtgga gctcctccgc ggtgaggacg tagggccgta cgaagttgtc
     8221 gtagggctcg aagggccaga gatcgtcgac gggcttgcgg ggcagtgggg gtggcggacc
     8281 ggggtccaac ggctgggagt cgccggtgcg ttgggcgggg cgcggccccg gcgtcgacca
     8341 cgtccgggga gcagtacgtc gcagagccac gtgacgaatg cgcgcatggc ggtgcctcct
     8401 gattcttctc agggttaatt cagggtcgct ttgacgagcg gtgattaccg ttcgtgtctc
     8461 gatggtcact gctgcgacgt acgctgcgga aggggtctgg cgttgcctgg agcgccaggc
     8521 aacggtgagg ggtgacatga gcgaacgggt tgacatggac gccgagtcgg ggcgcgccgt
     8581 actgggaagg acgcttcggc tccttcggga gaaggcgggc aaatcactgg ggcagttggc
     8641 cgacgagacc gggtacgact agagctatct gagccgtctg gagaagggcg agagggtctc
     8701 caatctgacg gtgatgaagg acctggacgt gtactacggg gccggtgatc tgctggttag
     8761 ccactggaag gtggctcggc tggacgcgtt caaggatcag tacaagcggt tcatggagct
     8821 ggaagcgacg gctcggatca tgtggaagtt cacgctcggc attcctgggc tcatgcagat
     8881 cgaggacttc gcccgagggg tgttgtctgg ggcccagaca acagctgacg ttgacgaagc
     8941 ggtcgaggaa caggtggctg cccggcttgg gcggcagtac ctcctgcggc agaagcccga
     9001 gcccgacctt cggatcatca tggacgagtt cgcgctacgg cggcccgctg ccggtggcaa
     9061 gacctggcac gaccaactgc ttcaccttga gacagctgcg aggtggccca acttgacgct
     9121 ccaagtactc ccgttctctg cgggggcgca ccaccatatg aacgggtcgc tgaccctgct
     9181 ctggcagagg gacggaagtg ccgttgccta caaggaaggc aacggttgca gtcggctgat
     9241 cgaggattcg gacgaggtcc tgcggcagcg attgtcctac gatcggctcc gcgatcttgc
     9301 gctgtccccg ccggattcgc tcgcgctcat caaggacgca ctgaaggagc acagatcatg
     9361 acgcgcaccc ttgacctcag cactgccgtg tggcgcaagt cgtcctacag cgaaggtggc
     9421 gacaacgact gcgtcgaggt cgccgacggc cagcccggca tagtccccgt ccgggacagc
     9481 aaagtcccgg acgggcgcgc cctgctcgtc tccgccgcat cctggtcggc cttcgtccgc
     9541 agcgtgaagg cagccccgtg acacccgacc tcaccaccgc cgtgtggcgc aagtcgtcct
     9601 acagcgacgg gggagccaac aactgcctcg aagtcgccga cggctacata ggcaccgtcc
     9661 ccgtccggga cagcaagcgc ccggacgctc accccttgct cctctccgcc gcatcctggt
     9721 cggccttcgt ccgcagcgtg aaggcagccc cgtgacaccc gacctcacca ccgccgtgtg
     9781 gcgcaagtcg tcctaccgcg acggcggcgg cacgaactgc ctcgaagtcg ccgacggcca
     9841 cccgggcata gtccccgtcc gggacagcaa agtcccggac gggcgccctc tgctcgtctc
     9901 cgccgcggcc tggtcggcgt tcgtgggcgg ctacgcaccc ggagctggtc aggggcggta
     9961 ggccgcgcgg ttgtggtagg tgaccttgcc ggtggggagc ttggtggggg cgaagagttc
    10021 gtcgacggtc acgaagtgga agccgcgggc tttgaggccc ttgatgatgc caggtatcgc
    10081 gttgaccgtc gtcttgtgga tgtcgtgcat gaggacgatg tcgccgggcc gggtcgtctc
    10141 attcacggtc ttgacgagcc gggcggtgtc gcggtgcttc cagtcgaggg tgtcgacgct
    10201 ccagtgc
//
