LOCUS       NZ_NIUP01000024        10270 bp    DNA     linear   CON 19-MAR-2024
DEFINITION  Bacillus mycoides strain MOD1_Bc228 Bc228_contig_24, whole genome
            shotgun sequence.
ACCESSION   NZ_NIUP01000024
VERSION     NZ_NIUP01000024.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus mycoides (Bacillus weihenstephanensis)
  ORGANISM  Bacillus mycoides
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 166425)
  AUTHORS   Chase,H., Gopinath,G., Tall,B. and Carter,L.
  TITLE     Bacillus cereus isolated from various food and clinical sources
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 166425)
  AUTHORS   Chase,H., Gopinath,G., Tall,B. and Carter,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUN-2017) US FDA, CFSAN, 8301 Muirkirk Road, Laurel,
            MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-DEC-2016
            Assembly Method                   :: CLC NGS Cell v. 8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002200005.1-RS_2024_03_19
            Annotation Date                   :: 03/19/2024 02:45:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,644
            CDSs (total)                      :: 5,560
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 84
            rRNAs                             :: 6, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 91 of 200
            Pseudo Genes (incomplete)         :: 129 of 200
            Pseudo Genes (internal stop)      :: 46 of 200
            Pseudo Genes (multiple problems)  :: 56 of 200
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:21:01
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 90683
            Orig. end                         :: 100953
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            NIUP01000024.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10270
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10270
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10270
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5270](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5270)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    19..33
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.81"
                     /tool="antismash"
     gene            complement(972..1415)
                     /locus_tag="CEU84_RS23210"
     CDS             complement(972..1415)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757615.1"
                     /locus_tag="CEU84_RS23210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NUDIX hydrolase"
                     /protein_id="WP_002085308.1"
                     /transl_table=11
                     /translation="MKKVNVTYALLYDETHEKLLMVKNKGENGSYYTLPGGAVKFGETL
                     EEAAIREVKEETGLDISVKGVCSISEAFFEERGHHAIFFNFLGEITGGEICISRPKEIE
                     EITWMELDSAEPYLRIPEHLKSLLQKKETVPYIFNGTIIHQSS"
     gene            complement(1600..2016)
                     /locus_tag="CEU84_RS23215"
     CDS             complement(1600..2016)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016104977.1"
                     /locus_tag="CEU84_RS23215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_002085307.1"
                     /transl_table=11
                     /translation="MQIVKQWVQEDSDYIREKVIEYNQKQLSNEEKTPSEKISFIVKNE
                     KEEIVGGITAITFWHHVYVDFLWVSEEYRHEGYGSNLMNLIEEFAIKKECRLIKLDTFS
                     FQAPNFYKKHGYKVIGVSEDYPKGHNHYYLEKRL"
     gene            complement(2197..3405)
                     /locus_tag="CEU84_RS23220"
     CDS             complement(2197..3405)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1137: Major
                     facilitator superfamily MFS 1"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814376.1"
                     /locus_tag="CEU84_RS23220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="OFA family MFS transporter"
                     /protein_id="WP_002127613.1"
                     /transl_table=11
                     /translation="MKQTSINPLLIVLGTIIVQIGLGTIYTWSLFNQPLVSKFGWNLNS
                     VAITFSITSFSLSFSTLFAGKLQQKFGLRKLIATAGIVLGLGLILSSQVSSLPLLYLLA
                     GVVVGYADGTAYITSLSNLIKWFPNRKGLISGISVSAYGMGSLIFRYINGNLINSLGVS
                     QAFLYWGIIVLLLVLIGSFFLREAIVSNTATETLHNDYTPREMMKTKQVYLLFFMLFTS
                     CMGGLYLIGMVKDIGVQLVGLSAATAANAVAMIAIFNTIGRIILGTLSDKVGRLKIVSA
                     TFIIIGLSVFTLSFIPLNYGIYFACVASVAFCFGGNITIFPAIVGDFFGLKNHSTNYGI
                     VYQGFGFGALAGSFIGALLGGFQPTFITIGVLCVISFVISIVIRPPNAEKKKEIKSLNR
                     KIA"
     gene            complement(3684..3827)
                     /locus_tag="CEU84_RS29605"
     CDS             complement(3684..3827)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017657232.1"
                     /locus_tag="CEU84_RS29605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002013225.1"
                     /transl_table=11
                     /translation="MKNEVNKNKKQNIKDVNTEQNAIYSDPKDAANMQTVPQPKDFEEI
                     EY"
     gene            complement(3859..4887)
                     /locus_tag="CEU84_RS23230"
     CDS             complement(3859..4887)
                     /EC_number="3.1.1.103"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1053: beta-lactamase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002085304.1"
                     /locus_tag="CEU84_RS23230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="WP_016127063.1"
                     /transl_table=11
                     /translation="MYKYEKLISWVESIKETNKSSAAALCIIKDNKIVLEHYSGTHSNT
                     PTSKKVTISSQFNVASARKSYLGLMVAYAVYEGKIKSLDDEAVNYFKEYDPSLLGKTTI
                     RHLVTHSHGLNETDDGTAFREFEPGQAWAYRDINVRMVTQLIYQLYNKSFPELLKERVF
                     TPADFKKTGWRIKQDDNLVKVVVNPDEDAISSVGAVDDGSEKNLFVSAREFAFWGNLHL
                     NKGFLNGKQIVPKEVIALATSLQSPEYTNKELPQNGLFWFVQNEPALFSELGERVPKGS
                     YQILGITGPTILVIPEYNVVVAKMYNKRYNYGGDNYLYYLREFSNLVADTFSSSKNTMV
                     RV"
     gene            complement(5001..5270)
                     /locus_tag="CEU84_RS23235"
     CDS             complement(5001..5270)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001869.1"
                     /locus_tag="CEU84_RS23235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_078203291.1"
                     /sec_met_domain="TIGR03601 (E-value: 5.4e-44, bitscore:
                     141.9, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNEFQQELQALNLNAYQPGNVVYWDQQQNQYPYYYIQDDARRCGG
                     CGGRCGGCGGRCGGCGGGRCGGCVGCVGCVGCFSCSNCWNWWFI"
     misc_feature    5284..5299
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 26.71"
                     /tool="antismash"
     gene            complement(5470..5916)
                     /locus_tag="CEU84_RS23240"
     CDS             complement(5470..5916)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836819.1"
                     /locus_tag="CEU84_RS23240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_000836819.1"
                     /transl_table=11
                     /translation="MKSKFHHIVRAIMIKDEKLLVAEYIGHHYFLPGGHVEIGESAENA
                     LIRELREELGVTYSIKQFLGVIENQWQAKETLHHEINHIFEIDSNELKSDFTPISKESH
                     LAFHWIDFNKENINSYTIMPTPSVKELLEKKLSDELLTCWISNF"
     gene            complement(6289..6858)
                     /locus_tag="CEU84_RS23245"
     CDS             complement(6289..6858)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002065694.1"
                     /locus_tag="CEU84_RS23245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4256 domain-containing protein"
                     /protein_id="WP_000665071.1"
                     /transl_table=11
                     /translation="MKESNKKELSLEQREELLQTLKDRFENNMNRHKGLEWANVQERLD
                     ANTEKLWSLNEMERTGGEPDVVDFGKEKGEYIFYDCSAESPKGRRSVCYDLEALESRKK
                     HKPENNAIDMATAMGIELLTEEEYRTLHNLENFDMKTSSWVQTPSDIRELGGALFCDYR
                     FGHVFVYHNGAESYYAARGFRGSLRV"
     gene            complement(7042..7506)
                     /locus_tag="CEU84_RS23250"
     CDS             complement(7042..7506)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136577.1"
                     /locus_tag="CEU84_RS23250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_088291535.1"
                     /transl_table=11
                     /translation="MNVQLKIVTRENWEDALKLRVKENQIHLVPAVAVSLAKVYIKPDG
                     ENVEYIPFAIYDGDLIVGFIMHAFVRGSTDMYWINGFIIDEKEQGKGYGKAALQEIINL
                     IKNNFKECEEIRLTVHKDNISAKKLYECYDFKPLGNVYDGEEVYRLLLLK"
     gene            complement(7584..8507)
                     /locus_tag="CEU84_RS23255"
     CDS             complement(7584..8507)
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016087665.1"
                     /locus_tag="CEU84_RS23255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleoside hydrolase"
                     /protein_id="WP_002127616.1"
                     /transl_table=11
                     /translation="MEKVLFFGDPGIDDSFAIMYGLLHPEIEIVGIVTGYGNVEHIHAA
                     HNAAYILQLANRQDIPVISGATGPLTNEITTYYPEIHGPEGLGPIHVPESVLSIPIYNF
                     GSIAAILVKYGEDLTIVDVGRSTSLAIAFNLWNELMLNIKGIYFMGGVFLEAGNVTPLA
                     EANVYGDPIASQIVFQQAKNITLFPLNVTNKTVLTPSVFTYILVNTQNPFKSIMKPMYD
                     YYLSAYQKLNPSIEGPLLHDVLAISGLVNPTFLNYTSRKVTVDICGETKGQTFADFRPH
                     SKSEGARIALQLDEEKFIQDFMKIML"
     misc_feature    8757..8773
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.88"
                     /tool="antismash"
     gene            complement(8791..9702)
                     /locus_tag="CEU84_RS23260"
     CDS             complement(8791..9702)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136580.1"
                     /locus_tag="CEU84_RS23260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DMT family transporter"
                     /protein_id="WP_002185732.1"
                     /transl_table=11
                     /translation="MHNKKWDFRIVCAHAFTILIWGTAFPGIRMGLEAYTPEHLTLLRL
                     LIASLILFLFSLIYKLRLPDLKDIPVIFIFGALGFTIYHIALNYGEKSVNAGPASLIVS
                     VTPILTAILASVFLNERMKLNGWIGGVISFVGIAFLSLSQGDSIQLNSGGLLILLAAIS
                     ESLFFVFQRPYLKKYGFLPFTIYTIWSGTVFMLIFLPGIYQEIVTAPIEITLSVIYLGV
                     FPTVLPYIALAYITSHASASEATSSLYLTPVTACIIAWIWLGEVPTFVSIIGGGITILG
                     VVIAHLTFNKDDYKQIESEKVW"
ORIGIN
        1 tgttgtgtct cggatatttt accaatttga gaagcgttgg aagaaatttg tattgtgcat
       61 tcgtcgatga tttctaagtt aacgttgttg tttgcccatt cactagccaa taaacttgac
      121 cgatctacac cttgtaaaat cttttttcta tctatattaa taatcgtttg ggattcattt
      181 ggaattaggc cggatatatt aggatatttc ccttcaataa gccttgaata caacgtaatt
      241 gtaccgaatt taaaaataat gtgactctct gaaagatata tgtatactaa atttgaatta
      301 ctgctcatta attttagtag ttcactaata gtggaacttg gtacaatgca atttaatttc
      361 gcatgtgaag aaatgggtat ttcacgtaaa gccagtctgt gcgaatcagt agcagcacaa
      421 attaatttgt tatgatcaat aacgaaatgt acgccagtaa gaactggtct agattcattt
      481 ttagcagttg caaagacagt ttgtttgaat acctcaatca gctgttccgt ttctacttgt
      541 atttctgtat gtgagtctaa aaatggtaca ttaggaaatt catttgccga gagtccattt
      601 aagtttaaag taatttcatc tgattgaatt gtaatcaact gctcattcat actttttatt
      661 aaaatctcac ttggcatttt cttaataatt tcaatgaaat actttgctgg tacaacaata
      721 cttcctgctt ttaaaatagt tgtaatttgt tcgtcttcaa ttgaaatagg tataaatttt
      781 tcaatgaaaa tattcgaatt acttgcgatt aaagtaattc cagattgatc tgctgttatc
      841 tttattccgg ataatatagg aattaaagtt tttgttgaga tagctttact tacttctgaa
      901 agagcttgtg taaattgttt gtgattaacg ataaactcca tttttccccc gccttaaatg
      961 aaatactatc tttatgaaga ttgatgaata atagttccat taaaaatata aggaactgtt
     1021 tctttctttt gtaaaaggct ttttaagtgt tctggtatac gtaaataagg ttctgctgaa
     1081 tctaattcca tccaggtaat ctcctcaatc tcttttggcc gtgatataca gatttcccct
     1141 ccggttattt ctcctaaaaa attaaagaag attgcatgat gtcctctctc ctcaaaaaag
     1201 gcttcactaa tggaacaaac cccttttacg gatatgtcta gccctgtttc ttctttcact
     1261 tcacgaattg ctgcctcttc taacgtttcc ccgaatttaa ctgctccacc tggtaaagtg
     1321 taataagaac cattttcccc tttgttttta accattaata gcttttcatg agtttcatca
     1381 tatagaagtg cgtatgtaac atttactttt ttcatatgta acccccttga atggtgaaaa
     1441 ttttctgtct ttattttatt ctatcattaa attgttttga ttcataaaaa gtaatttgag
     1501 taaataacta atacgaaacg atatcgtgtt catagacaca caaaagaaaa gagcatattt
     1561 tgaaaaagat taatcataat acactcttta atgattcatt tataaccttt tttctaaata
     1621 gtaatgatta tgccctttag gataatcttc acttacccct atcactttat atccatgctt
     1681 cttataaaaa ttaggggctt ggaaactaaa agtatctaat ttgattagcc tacattcttt
     1741 ttttattgca aattcttcaa taagattcat taaattgctt ccataacctt catgcctgta
     1801 ttcttcagaa acccatagga agtcaacata tacatggtgc caaaatgtga ttgcggttat
     1861 ccctcctact atttcttctt tttcattctt cactataaaa ctaatctttt ctgaaggtgt
     1921 tttttcttca tttgaaagtt gtttttggtt atattcaatt actttttctc ttatgtaatc
     1981 actatcttct tgtacccatt gctttactat ttgcatttta actatcacgc ccctttacat
     2041 tatttattca atttaataat cggaatttcc tatcttcttt aatgttagat tatgaaataa
     2101 gtacagggaa aaaattagat tttttgaatg tcattcattg tttatacatt tttttgtaaa
     2161 gcaaaagagc cctagtataa ggactctttc ttagcattaa gctatttttc gatttaacga
     2221 tttgatttcc ttcttctttt ctgcatttgg agggcggatt actatagaga taacgaaaga
     2281 tataacacat agaacaccga ttgtaatgaa ggttggctga aatccgccaa gtagtgctcc
     2341 aatgaatgat cctgcaagtg caccaaatcc gaaaccttgg taaacaatcc cgtagtttgt
     2401 actatgattt tttaatccga agaagtctcc cacaattgct gggaagatag tgatgttacc
     2461 accaaagcaa aaggcaacac ttgctacaca agcaaaataa attccgtaat ttagtggaat
     2521 aaaacttaaa gtgaagacgg ataatccaat aataataaat gtagccgaga cgatttttaa
     2581 tcggcctact ttatctgata acgttccaag aataattcta cctattgtat taaagattgc
     2641 aatcattgcg acggcattag cggccgtcgc tgcactaaga ccaacgagct gcaccccgat
     2701 gtcttttacc ataccgatta aatacagtcc gcccatacat gatgtgaata acatgaaaaa
     2761 taagagatat acttgtttcg ttttcatcat ttcacgcggc gtataatcat tgtgtaatgt
     2821 ttcagttgca gtgttactta caattgcttc acgtaagaag aatgatccta tcaacactaa
     2881 tagtaagacg ataatacccc aatataaaaa tgcttgtgat actccaagac tgttgataag
     2941 atttccgttt atatatctaa agattaagct tcccattcca tatgccgaaa cagatatacc
     3001 agaaataagt cctttacggt ttggaaacca tttaattaaa ttagatagtg atgtaatata
     3061 tgctgttcca tcagcgtatc caacgacaac accggctaat aaataaagta atggtaatga
     3121 ggaaacttgt gaactaagta ttaaaccgag tcctaaaaca attcctgcag tagcgataag
     3181 tttacgaagt ccaaattttt gttgtagctt tcctgcaaat agagttgaga atgataaaga
     3241 aaaactcgta attgagaacg tgatcgcgac tgaattgagg ttccatccaa acttacttac
     3301 aaggggttga ttaaataaac tccatgtgta aattgttcct aggccaattt gaacaatgat
     3361 tgtacctaga acaattagca atggatttat agatgtttgt ttcatgctaa ctgtcccctt
     3421 cttttatcat gacatgaatt ttcacagtta gtatatccat gataaattgc aagatactgt
     3481 acggaaaatg gaaattgagt tagcttacac aaaaagacac ttcatcaaga agtgcctttt
     3541 tgtaggttat atacagcatt taaagaaagc tttgttaaga taagacattt tcgaatgctt
     3601 atgtatatat gttatcaaat aatttgctga aagtgatgag acttaagacg tatttttctt
     3661 tcataaataa gtcggagaga aaattaatat tcaatttcct caaagtcttt cggctgcggt
     3721 acagtttgca tattagctgc atctttcgga tcgctataaa tcgcgttttg ttctgtgttt
     3781 acatccttta tattttgctt tttattttta ttcacttcat ttttcattaa agttcactcc
     3841 tttttattat aggagtagtt ataccctaac catagtattt ttactgctac taaatgtatc
     3901 agcgactaaa ttgctaaatt cacgtaaata atataaataa ttatccccac catagttgta
     3961 tcttttattg tacattttag caacgacgac attatattct ggaatgacta atatcgttgg
     4021 tcctgtaatt cctaatattt gataggaacc tttaggaact cgctcaccaa gttcgctaaa
     4081 taaagcaggt tcattttgaa cgaaccaaaa caacccgttt tgaggtagtt ctttatttgt
     4141 atactctgga ctttgtaagc tcgtagcaag tgctataact tcttttggca caatttgttt
     4201 accatttaaa aaccctttat ttaaatgaag gttaccccaa aatgcaaatt ctctcgcaga
     4261 gacaaaaaga tttttctcgg agccgtcatc tactgcacca acactactta ttgcatcttc
     4321 atctgggttt acaacaacct taactaaatt atcatcttgt tttattctcc atccagtttt
     4381 tttgaaatca gcaggtgtga atacacgctc ctttaatagt tcaggaaaac tcttattata
     4441 cagttgataa ataagctgcg tcaccattct tacattaata tctctgtacg cccaagcttg
     4501 tcccggctca aattcacgaa aagctgtccc atcatcggtt tcatttaaac cgtgtgaatg
     4561 agttactaaa tgtcttatcg ttgtctttcc aagtaatgaa ggatcatatt ctttaaaata
     4621 atttacagcc tcgtcatcaa gagattttat tttcccctca taaactgcat aagctaccat
     4681 taatcctaaa tagctttttc ttgcggaagc gacattgaat tgtgaagata tagttacttt
     4741 tttactagta ggagtatttg aatgcgttcc actataatgc tctagtacaa tcttattatc
     4801 tttaataata caaagtgctg ccgcagaact tttattggtc tctttaatgc tttctaccca
     4861 tgaaattaat ttttcatatt tgtacaaata aatatccccc taaaacatat aaaaataaca
     4921 gcatgaaaga aaagtttcaa tacattataa ctttgcatct gcacttttct ttcatgtgaa
     4981 atattataga tctataatac ttaaataaac caccagttcc agcaattaga acagctaaaa
     5041 caaccaacac agcctacaca acctacgcaa ccgccacaac gtccgccgcc acaaccacca
     5101 cagcgaccac cacaaccgcc acaacgtccg ccgcatccgc cacaacgacg tgcatcgtct
     5161 tgaatgtagt aatatggata ttgattttgt tgctgatccc aatacacaac attaccaggt
     5221 tgataagcat taaggtttaa cgcttgtagt tcttgttgaa actcattcat tatgaaaccc
     5281 tccatttaca aatgtaaatt ccgtccaaat tttcgagttt atttactttt aaaaataaag
     5341 tagaaataag gctatagact aaatgcgtac atcaacaaaa tatgacttta cactaggtga
     5401 tgtaccttgt ttatgagcct attttcttta tattctttta gtggacggaa ttaatataaa
     5461 tagagacaat taaaaattac taatccagca agttaatagt tcatcactta attttttttc
     5521 tagtaattct tttactgaag gtgtcggcat tattgtatag gaatttatat tttctttatt
     5581 aaaatcaatc cagtgaaatg ctaaatggga ctctttagat attggagtga aatcggattt
     5641 taactcattt gaatctattt caaaaatgtg gttaatttca tggtggaggg tttctttggc
     5701 ttgccattga ttttctatga cgcctaaaaa ttgttttata ctgtaagtta ctccaagttc
     5761 ttctcgtaac tctcttatta gtgcattctc tgctgattcc ccaatttcaa catgaccacc
     5821 gggtaaaaaa tagtgatggc ctatgtattc agcaactagt aatttttcat cttttatcat
     5881 gatagctcgt acaatatgat ggaatttact tttcattttg tattcctcct actcattcac
     5941 tttatacttg ctcgaaaatt ttcatttaaa agcgggataa tataaatcat aggtgtttca
     6001 tatgggtgaa tttcattgat aattgcaaga gtacctttta caagttctcg tttatacgtt
     6061 aattaaaact aataattttg ataaatgatt ctaaaaaaga gtatgtttat gaagaaaatt
     6121 atcaaaacat actcttaata tttttagttg attcattctt ttacaataag gtttgattag
     6181 ccctattctt tgcggcctca aaatcgaagc cgtagctaaa gtatgcaatc actactattc
     6241 cctaagcaaa tctattctct acttcaaatt tacctgcctg tacaaatttt agactcttaa
     6301 cgaaccacga aaacctcttg cggcatagta ggattctgct ccattgtgat acacaaagac
     6361 atgtccaaag cgataatcgc aaaaaagggc accgccaagt tctctaatat cagaaggtgt
     6421 ttgcacccaa ctcgacgtct tcatatcgaa attttcaaga ttatgtaatg tacgatattc
     6481 ttcttccgtt aatagttcaa tacccatggc agttgccata tcaatagcgt tattttctgg
     6541 tttatgtttc tttcttgatt ctagcgcttc aagatcgtaa caaacacttc tacgaccttt
     6601 aggactttct gctgaacaat cataaaaaat atattcgcct ttttctttac caaaatcaac
     6661 aacatcaggt tcgccaccag ttcgttccat ttcgttgagc gaccacaact tttcagtatt
     6721 agcatccagc ctttcttgaa cattagccca ttctaaacct ttatgacggt tcatattatt
     6781 ctcaaaacga tctttcaatg tttgtagtaa ttcttcacgt tgctctagtg acaactcttt
     6841 tttattgctt tctttcatat tagttcctcc aatgatgttt ttattaatga tagtttaagt
     6901 atatataaat actgttttat aataaaatgt ttaaataatt gcgtgaattg agtttgcgat
     6961 aatttatatg aattagtaca accaattctc cgcaatatga ccctttaacg gaacaactta
     7021 ttgtttcaaa acagtacatc tttatttcaa caacaataag cgatatactt cctcaccgtc
     7081 atatacattt cctaatggtt taaaatcata acattcatat agtttctttg cagaaatatt
     7141 atctttatgg acagttaatc taatttcctc acactcttta aagttatttt ttattaagtt
     7201 aatgatttcc tgtaatgctg cttttccata tccttttcct tgctcttttt catcaatgat
     7261 aaatccgtta atccaataca tatctgttga ccctcgtaca aaagcatgca taataaaacc
     7321 aactataagg tcaccatcat atatagcgaa tggaatatat tcgacatttt caccatctgg
     7381 ttttatgtat actttagcaa gtgatactgc cactgcggga acaagatgga tttgattttc
     7441 tttaactcgt aactttaatg cgtcttccca attttctctc gtaacaattt ttaattgaac
     7501 gttcactatt ttaagctcct ttaaaaagag aatttatctt ttatattcaa cggagtgaaa
     7561 tgattccctt tttcgacatc aacttataac atgattttca taaaatcctg aataaatttt
     7621 tcctcatcta attgtaacgc tatacgcgcc ccttcagatt tagagtgtgg gcgaaaatca
     7681 gcaaaagttt gtcccttcgt ttcgccacat atatccacag tcacttttcg ggatgtataa
     7741 tttaaaaatg taggattcac taaaccgctt atagcaagta catcgtgtaa taatggccct
     7801 tcaatagatg gattgagttt ttggtatgct gataaataat aatcgtacat gggtttcata
     7861 atcgacttaa atggattttg tgtattaact aaaatgtaag taaatacact tggggttaaa
     7921 accgttttat ttgttacatt taaagggaat aacgttatat ttttcgcttg ctgaaataca
     7981 atttgacttg ctataggatc tccgtacacg tttgcctctg ctaatggagt aacattccct
     8041 gcttctaaaa agacaccacc cataaaataa attcctttta tatttaacat caattcgttc
     8101 cataaattaa aagcaatcgc taacgaggta gatcttccta catcgacaat tgttaaatct
     8161 tctccatact ttacaagaat cgcagcaata ctaccaaaat tgtatatagg aatagataac
     8221 acactttccg gtacatggat gggccctaat ccttctggtc catggatttc agggtaatac
     8281 gttgtaattt catttgtaag tgggcctgtt gcaccgctaa ttacaggaat atcttgtctg
     8341 tttgctaact gtaaaatata tgcggcatta tgagcggcat gtatatgttc aacatttcca
     8401 tatccagtta cgataccaac aatttcaatt tcaggatgca gtaagccata cataattgca
     8461 aacgaatcat caatacctgg gtctccaaaa aataaaacct tctccaagaa atgcactcct
     8521 ttcataggta agataattat gtatatgtgg aaaaaaatta aatctataca gttctaagca
     8581 aatattaatg gtgagtaatg tattgataaa aaagaactag cattatgcta gttcttttgc
     8641 agataaataa tctaccagtt taattaaatc tcgtacaaat attggactaa ctgattttaa
     8701 tatagatttt tttcactata tttatagaat tgaaaacctg ttattaatcc gaaattttgc
     8761 attcaaattg caaatactcg tagggatgtc ttaccatacc ttttcactct caatttgctt
     8821 ataatcatct ttattaaatg ttaaatgagc aatcacaact ccaagtatag taattccccc
     8881 tccaattatc gaaacaaagg tcggcacctc acctaaccat atccaagcga taatacatgc
     8941 agttactggt gttaaatata gagaacttgt tgcctcagaa gcactggcat gggatgtaat
     9001 atatgctaat gcaatatacg gcagtactgt cgggaacaca cctaaatata taacactcaa
     9061 agtaatttct ataggagctg ttacgatttc ttgataaatc cctggcaaaa aaataagcat
     9121 aaatacagta ccagaccaaa ttgtatatat tgtaaatggc aagaaaccgt acttttttaa
     9181 gtatggcctt tgaaaaacga aaaatagact ttctgaaatt gccgccaata atattagtaa
     9241 tcccccacta ttcaattgaa tggaatctcc ttgacttaac gataaaaaag caattcctac
     9301 aaaactaatt acaccaccga tccaaccatt taatttcatt ctttcattca aaaaaacaga
     9361 agcaagaatt gccgttaata taggtgttac tgatacaatt aaactagcag gtccagcatt
     9421 cacactcttt tcaccgtagt tcagtgcaat atgataaatt gtaaatccaa gagcaccaaa
     9481 tataaaaatt actggaatat ctttcaaatc aggtaaacgt aacttgtata taagtgaaaa
     9541 cagaaaaaga atcaaagaag cgattaataa tcgtagcaaa gtaaggtgtt caggggtgta
     9601 agcttcaagc cccatccgaa ttcctggaaa agctgttccc catataagaa tcgtaaaagc
     9661 gtgagcacat acaatacgaa aatcccattt tttattatgc atatattctc tccctctcca
     9721 ataaaccagc cattttctaa ttaccaatta tttcttttca tcatatttaa tagttgatac
     9781 aatgtcttca accagttaat cctcgaaaaa accaaccact ttgcattttt aaaatgaatg
     9841 agggagtttt taatatatct actgataaaa aactgcctaa atatcggcag ataatagact
     9901 atatgaaaga aaaaattgaa tacggagaat ggccgattgg aagtaaggtt cctagccagc
     9961 ggcagttagc gaaattattt catgtaaatc gtagtactgt aataattgca ctagaagaac
    10021 ttatggcaga tggcttaatt caagggaaaa tcgggtcagg aacaattgta acgaataata
    10081 catggtcatt attagccacc aatcccccgc ccgattggag tgaatatgta aaggctggta
    10141 ttcataaacc aagcaagtta atggtacgag aaataaatga agcagaagca aataaaaaat
    10201 tgattcatct tagtaaagga gaacttgcac ctcatctttt cccgttagaa actatgcaat
    10261 tcattatgcg
//
