LOCUS       NZ_CP021744            10399 bp    DNA     linear   CON 16-JAN-2024
DEFINITION  Streptomyces albireticuli strain MDJK11 chromosome, complete genome.
ACCESSION   NZ_CP021744
VERSION     NZ_CP021744.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces albireticuli
  ORGANISM  Streptomyces albireticuli
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8144417)
  AUTHORS   Wang,Y., Du,B., Ding,Y., Liu,H., Hou,Q., Liu,K., Yao,L. and Wang,C.
  TITLE     Streptomyces albireticuli Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8144417)
  AUTHORS   Wang,Y., Du,B., Ding,Y., Liu,H., Hou,Q., Liu,K., Yao,L. and Wang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUN-2017) Department of Mcrobiology, Shandong
            Agricultural University, No.61 Daizong Street, Taian, Shandong
            271018, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Celera Assembler v. 2.3
            Expected Final Version            :: yes
            Genome Coverage                   :: 88.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/16/2024 07:12:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,975
            CDSs (total)                      :: 6,876
            Genes (coding)                    :: 6,711
            CDSs (with protein)               :: 6,711
            Genes (RNA)                       :: 99
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 74 of 165
            Pseudo Genes (incomplete)         :: 97 of 165
            Pseudo Genes (internal stop)      :: 5 of 165
            Pseudo Genes (multiple problems)  :: 9 of 165
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:17:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5846378
            Orig. end                         :: 5856777
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP021744.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="26"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(745..1416)
                     /locus_tag="SMD11_RS25300"
                     /old_locus_tag="SMD11_5127"
     CDS             complement(745..1416)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180689.1"
                     /locus_tag="SMD11_RS25300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMD11_5127"
                     /product="response regulator transcription factor"
                     /protein_id="WP_087928634.1"
                     /transl_table=11
                     /translation="MPDTRPIRIVLADDEHMVRSALRAILHSEPGLEVVGEATDGAEAV
                     AVIRELRPDIVLMDVRMPGIDGIRATEQVLATMDTPPRVIVVTTFENDSYVYDALRAGA
                     AGFLLKRAGADELVQAVRLVARSDSLLFPAAVRALAAEHAGARDTRDAAERLRARLSER
                     EAEVLRLMAAGLSNAGIAARLTLGEATVKTHVAGTLAKLGVSNRTQAVIAAYESGFVSP
                     R"
     gene            complement(1522..2622)
                     /locus_tag="SMD11_RS25305"
                     /old_locus_tag="SMD11_5128"
     CDS             complement(1522..2622)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238577.1"
                     /locus_tag="SMD11_RS25305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMD11_5128"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_087928635.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 4.2e-16, bitscore:
                     57.7, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDSGVLHIKGRVLVGADPEGGVRDELWAVDGRITFERPAGAREV
                     TTIEGWALPGLVDAHCHVGLDAHGPVDESTSEKQALTDRDAGTLLIRDAGSPSDTRWID
                     DREDLPRIIRAGRHIARTRRYIRNYAHEIEPEDLVAYVAREARRGDGWVKLVGDWIDRE
                     TGDLSSCWPRGEVEAAIAEAHRLGARVTAHCFAEESLAPLVEAGIDCVEHATGLTEETI
                     PLFAERGVAIVPTLVNIATFPRLAEGGQRKFPRWADHMRRLHARRYETVRAAYDAGIPV
                     FVGTDAGGSLAHGLVAQEVAELVKAGIPATAALAATAWDARAWLGRPGLEEGAPADLVV
                     YGSDPRADVRVLAAPRRVILRGRVVR"
     misc_feature    2745..2759
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.69"
                     /tool="antismash"
     misc_feature    2852..2867
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    2988..3000
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            3025..3591
                     /gene="ectA"
                     /locus_tag="SMD11_RS25310"
                     /old_locus_tag="SMD11_5129"
     CDS             3025..3591
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577188.1"
                     /locus_tag="SMD11_RS25310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMD11_5129"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_087928636.1"
                     /transl_table=11
                     /translation="MTAAQADLARAHSEFLEMPEGLTLDTPRVEDGAAIWRMARDSGAL
                     DLNSSYSYLLWCRDFAATSAVARRADGRPAGFVTGYLRPERPGTLVVWQVAVDHGHRGR
                     GLAGALLDGLVARVARERDVHAVETTVTPDNTASNSLFASFAARHGARVDREVLFDDGL
                     FPGGGHAPEVLHRIGPLDFPAAPAR"
     gene            3655..4923
                     /gene="ectB"
                     /locus_tag="SMD11_RS25315"
                     /old_locus_tag="SMD11_5130"
     CDS             3655..4923
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 6.5e-72.
                     Score: 234.3. Matches aSDomain:
                     nrpspksdomains_SMD11_RS25315_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="SMD11_RS25315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMD11_5130"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_087928637.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.4e-74, bitscore:
                     249.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFEALESEVRSYCRSWPAVFDRAQGSRMYDEDGHTY
                     LDFFAGAGALNYGHNNPVLKRALLDYLERDGVTHGLDMGTTAKRAFLESFQDIVLRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYLDGQVPDFLWFERLLEDQGSGLNQPAAVIVETVQGEGGINVARA
                     DWLRALADLCERRDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTLSKSIGGYGMPMS
                     LTLFKPELDIWEPGEHNGTFRGNNPSFVTAAAALNAYWADGQMEKQTLARGEQVARALD
                     AICAEHPGLGGRYRGRGLVWGLEFTDRARASAVAARAFELGLLVETSGPASEVVKLLPA
                     LTISPEELDEGLRTLARAVRETA"
     aSDomain        3739..4749
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SMD11_RS25315_Aminotran_3.1"
                     /evalue="6.50E-72"
                     /label="SMD11_RS25315_Aminotran_3.1"
                     /locus_tag="SMD11_RS25315"
                     /protein_end="365"
                     /protein_start="28"
                     /score="234.3"
                     /tool="antismash"
                     /translation="FDRAQGSRMYDEDGHTYLDFFAGAGALNYGHNNPVLKRALLDYLE
                     RDGVTHGLDMGTTAKRAFLESFQDIVLRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYLDGQVPDFLWFER
                     LLEDQGSGLNQPAAVIVETVQGEGGINVARADWLRALADLCERRDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTLSKSIGGYGMPMSLTLFKPELDIWEPGEHNGTFRGNNPSFV
                     TAAAALNAYWADGQMEKQTLARGEQVARALDAICAEHPGLGGRYRGRGLVWGLEFT"
     gene            5001..5399
                     /locus_tag="SMD11_RS25320"
                     /old_locus_tag="SMD11_5131"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="SMD11_RS25320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMD11_5131"
                     /product="ectoine synthase"
                     /protein_id="WP_087928638.1"
                     /sec_met_domain="ectoine_synt (E-value: 6.5e-54, bitscore:
                     179.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKARSGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHIEAVLCVEGEAELTDDETGEKHWIEPGTMYLLDGHERHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEG"
     gene            5403..6293
                     /gene="thpD"
                     /locus_tag="SMD11_RS25325"
                     /old_locus_tag="SMD11_5132"
     CDS             5403..6293
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013004613.1"
                     /locus_tag="SMD11_RS25325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMD11_5132"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_087928639.1"
                     /sec_met_domain="PhyH (E-value: 4.9e-37, bitscore: 126.9,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTATVSDLYPTRGTQEVPVPRRDPVVWSRPGAPGPIGDTELADYD
                     RDGFLTVDRLIGPDEVARYRAELDRLIADPAVRADERSIVEPRSQDVRSVFEVHRISEV
                     FAELVRDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPRMR
                     TVSVSIALTENLDTNGGLMIMPGSHRTFLGCAGETPRDNYKRSLRMQDAGTPSDAALTG
                     FAERHGVRLFTGPAGSATWFDCNCMHGSGNNITPFPRSNVFIVFNSVDNAAVEPFAAPA
                     RRPEFVGARDFTPVP"
     gene            complement(6343..7374)
                     /locus_tag="SMD11_RS25330"
                     /old_locus_tag="SMD11_5133"
     CDS             complement(6343..7374)
                     /GO_function="GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042: O-methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004943262.1"
                     /locus_tag="SMD11_RS25330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMD11_5133"
                     /product="methyltransferase"
                     /protein_id="WP_087928640.1"
                     /transl_table=11
                     /translation="MRRARDEQDHSAALDRILQLGLGFAVSKVLFSAVRLGLFTELAAR
                     PLPYEELRDRLGLHPRAARDFLDTLVALDLLERGPEGYGNTPVTARYLDRNRATYLGGF
                     LEMTDHRLYGFWGTLLDGLRTGEPQNEIKHGGDFFGTLYQDPVRRSEFLQAMTGLSMRS
                     AHALADAVDWSAHRVVADVGCAEGGTLGQILLRHPHLRGTGFDLESARGDFDRRAERFG
                     LADRLTFAAGDFFTDPLPRADALLLGHILHDWDLPTKRMLLRKAYEALPAGGVVIVYET
                     LIDDDRRTHLTGLLMSLNMLVETTGGFDYTGAECREWLAGAGFAESRVQHLAGPESMVI
                     ARK"
     gene            complement(7480..8529)
                     /locus_tag="SMD11_RS25335"
                     /old_locus_tag="SMD11_5134"
     CDS             complement(7480..8529)
                     /NRPS_PKS="Domain: Aminotran_5 (13-342). E-value: 1.4e-24.
                     Score: 78.6. Matches aSDomain:
                     nrpspksdomains_SMD11_RS25335_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059809.1"
                     /locus_tag="SMD11_RS25335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SMD11_5134"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_234366157.1"
                     /sec_met_domain="Aminotran_5 (E-value: 7.1e-18, bitscore:
                     63.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESLAPGRFAPETVYLNTAATGLLPTGAAAAMGEAIGVLAAGAPG
                     NVDFSATVDAARTAYARLVGVPAARVAAGGSVAVYCGLVAQSLPAGAEVLVAEGDFASL
                     VNPFHTRPGLRVRTVPLERLAEEVRPDTALVAVSAVQSADGRIAALGAISAAARAYGAR
                     TLVDVSQAAGWFPLDAAAHDFTVGVGYKWLMGPRGVAFLTVPEDLGALTPVFAGWVTGE
                     DPWLTCYGKVEPLASTARRFDENVAVLSYVGALHGLALVEELGVERIGAHDRALADRFR
                     AGVVALGLTPVPAEGSAIVAVPGVRGAAERPAAAGVQVSARAGRLRAAFHLYNSTADVD
                     RLLEVLARR"
     aSDomain        complement(7504..8490)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SMD11_RS25335_Aminotran_5.1"
                     /evalue="1.40E-24"
                     /label="SMD11_RS25335_Aminotran_5.1"
                     /locus_tag="SMD11_RS25335"
                     /protein_end="342"
                     /protein_start="13"
                     /score="78.6"
                     /tool="antismash"
                     /translation="VYLNTAATGLLPTGAAAAMGEAIGVLAAGAPGNVDFSATVDAART
                     AYARLVGVPAARVAAGGSVAVYCGLVAQSLPAGAEVLVAEGDFASLVNPFHTRPGLRVR
                     TVPLERLAEEVRPDTALVAVSAVQSADGRIAALGAISAAARAYGARTLVDVSQAAGWFP
                     LDAAAHDFTVGVGYKWLMGPRGVAFLTVPEDLGALTPVFAGWVTGEDPWLTCYGKVEPL
                     ASTARRFDENVAVLSYVGALHGLALVEELGVERIGAHDRALADRFRAGVVALGLTPVPA
                     EGSAIVAVPGVRGAAERPAAAGVQVSARAGRLRAAFHLYNSTADVDRL"
     misc_feature    complement(9334..9348)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.35"
                     /tool="antismash"
ORIGIN
        1 tgtcctcgaa ggacgcctcc agggggatcc ccgcgaggtg cacgaccgcg tcggcgcccc
       61 gcagcgccgc gcgcagcgcc gggccgtcgg cgagctcggc ggtgacggcc tccggcgcgc
      121 cctcgaccgg ccgcgcgtcg aagagccgca gccggtggcc gtagcggggc agccgcgcgc
      181 gcagcatcgt gccgatccgg ccggccgcgc cggtgagcag gaccgtacgg ggagcggtca
      241 tggcgacacg ctagggacgc gtgggacccg ggtcaaccat gcggaacggg gtggcggggc
      301 ggcctgacgc cgggggagac ggccgcgagg agcgcgggtg cgcaccccgt gcgcggacgc
      361 gcgtcacgtg cggcggcacg cggggtggcg gggcggcctg acgccggggt agacggccgc
      421 gaggagcgcc ggtgcacacc ccgcgtgcgg acgacacgcg gaatgcgccg cggcgggtgc
      481 ccggccgggg gtcggtgctt acccgcgtga gtgcgacgcg gccggttcgc cggtcgggcc
      541 acgttcgtcg cgcggtgccc catccgcgcg tgacgggcgg cgggcgcgct gcgccccggg
      601 gcacggcgct cacccgcgtg agagcgccgc ggccggtccg ccgggcccgg gccgcgctcg
      661 cggcgcgtcg gcccggcgcg agcccgcgcc gctgctcgcc cgcgcgtggg tgacgcgcgc
      721 tgtcccccgg cccggccggc ccgttcagcg cggggagacg aagccgctct cgtaggcggc
      781 gatgaccgcc tgggtgcggt tgctgacgcc cagtttggcg agcgtgcccg ccacgtgtgt
      841 cttgacggtg gcctcgccca gcgtcagccg tgccgcgatc ccggcgttcg acagccccgc
      901 cgccatcagc cgcagcacct ccgcctcccg ctcggacagc cgcgcccgca gccgctccgc
      961 cgcgtcccgg gtgtcgcggg cacccgcgtg ctcggcggcc agcgcgcgca cggcggccgg
     1021 gaagagcagc gagtcgctgc gcgccaccag ccgtacggcc tggaccagtt cgtccgcccc
     1081 cgcccgcttg agcaggaagc cggcggcccc cgcgcgcagc gcgtcgtaga cgtagctgtc
     1141 gttctcgaac gtggtcacca cgatcacgcg cggcggcgtg tccatcgtgg cgagcacctg
     1201 ctcggtggcc cggatgccgt cgataccggg catccggacg tccatcagga cgatgtccgg
     1261 gcgcagttcg cggatcacgg ccacggcctc ggcgccgtcg gtggcctcgc cgacgacctc
     1321 cagaccgggc tcggagtgga ggatggcgcg gagggcgctg cggaccatgt gctcgtcgtc
     1381 ggccagcacg atgcgtatgg ggcgggtgtc gggcatgggg gtcagcttat ggcgggcccc
     1441 cggggggctc ccgccgcgct cccgcgccgt cttcccggac gccgggcggg cctgaacgaa
     1501 gcgcggggac agccgcgttc gctaccgcac cacccgtccc cgcaggatca cccgccgggg
     1561 cgccgccagc acccgtacgt ccgcgcgcgg atccgacccg tagacgacca ggtccgccgg
     1621 ggcgccctcc tccaggcccg gccggcccag ccacgcgcgg gcgtcccagg ccgtcgccgc
     1681 cagggcggcc gtggccggga tgccggcctt caccagctcc gcgacctcct gggcgaccag
     1741 gccgtgggcc agcgagccgc ccgcgtccgt gccgacgaag accgggatgc ccgcgtcgta
     1801 ggcggcgcgc accgtctcgt agcgccgcgc gtgcaggcgg cgcatgtggt ccgcccagcg
     1861 ggggaacttc cgctgcccgc cctccgcgag ccgggggaac gtcgcgatgt tgaccagcgt
     1921 cggcacgatc gccacgcccc gctcggcgaa cagcgggatc gtctcctccg tcagccccgt
     1981 cgcgtgctcg acgcagtcga tgcccgcctc caccaggggc gcgagggact cctcggcgaa
     2041 gcagtgcgcg gtgacccgcg cgcccagccg gtgcgcctcg gcgatggcgg cctcgacctc
     2101 gccgcgcggc cagcaggagg acaggtcgcc ggtctcgcgg tcgatccagt cgccgacgag
     2161 cttgacccag ccgtccccgc gccgcgcctc gcgcgcgacg tacgcgacga ggtcctcggg
     2221 ctcgatctcg tgcgcgtagt tgcggatgta gcggcgggtg cgggcgatgt gccggcccgc
     2281 ccggatgatc cggggcaggt cctcgcggtc gtcgatccag cgggtgtccg agggggagcc
     2341 cgcgtcgcgg atcagcaggg tgccggcgtc ccggtcggtc aacgcctgct tctcgctggt
     2401 cgattcgtcg acggggccgt gcgcgtccag gcccacgtgg cagtgcgcgt ccaccaggcc
     2461 gggcagcgcc cagccctcga tggtggtgac ctcccgcgcc ccggcaggcc gctcgaacgt
     2521 gatcctgccg tcgacggccc acagctcgtc gcgcaccccg ccctcggggt ccgcgccgac
     2581 gagcacccgc cccttgatgt gcagcacacc actgtcgctc atgacggcac tgtatgcgca
     2641 gcggccccgc cgccccaggg gtgccgaagg ggtcgcaggg gcccgtgagg agcccgggac
     2701 ggctccggaa tttcctccgg cgacgacccg gaggtattcc tggaattctt ccggaattac
     2761 cgtgaataca gcttcccgga attctcctgc ccccggaatc cgaccttaaa cgcggtgatt
     2821 ttgtcgccac cccgataggg ggtgtccggc ctgtgatgca ctccacaacc gcgcccggtt
     2881 cgcccgataa aatacggacg aatgctgtta ttcttccggc gtctcgcgcg cccacgtatc
     2941 cccgcgcgat aacacacgac ccaccgcccg ggaaaggccg gacccgcatg caattttcga
     3001 tccggctggg taaattcact ccgcatgacc gccgcacaag cagaccttgc acgtgcccac
     3061 agtgaattcc tcgaaatgcc ggaggggctg acgctggaca ccccacgcgt cgaggacgga
     3121 gccgcgatct ggcgcatggc ccgcgactcc ggggcgctgg acctcaactc ctcgtacagc
     3181 tacctcctgt ggtgccgcga cttcgccgcc acctccgccg tcgcccggcg cgccgacggc
     3241 cgcccggccg gcttcgtcac cggctacctg cgccccgagc gccccggcac cctcgtcgtc
     3301 tggcaggtgg ccgtcgacca cggccaccgc ggccggggcc tggccggcgc cctcctggac
     3361 ggcctcgtcg cccgcgtcgc ccgggagcgg gacgtccacg cggtcgagac gaccgtcacc
     3421 ccggacaaca ccgcttccaa cagcctgttc gcctccttcg ccgcgcgcca cggcgcccgc
     3481 gtcgaccgcg aagtgctctt cgacgacggc ctgttccccg gcggcgggca cgcgcccgag
     3541 gtgctccacc gcatcggacc gctcgacttc ccggcagctc ccgcccgctg agcccgtccg
     3601 gccccgtccg tccgccgcgc cccctgccac ccccgcaccc cactggagtc cgccgtgacc
     3661 atcaccccgc ccgccctgag cgtcttcgaa gccctcgagt ccgaggtccg cagctactgc
     3721 cgcagctggc ccgccgtctt cgaccgggcc cagggcagcc ggatgtacga cgaggacggc
     3781 cacacctacc tcgacttctt cgccggcgcc ggagcgctca actacgggca caacaacccc
     3841 gtgctcaaac gcgccctgct cgactacctc gaacgcgacg gcgtcaccca cggcctcgac
     3901 atgggcacca ccgccaagcg cgcgttcctg gagtccttcc aggacatcgt gctgcgcccg
     3961 cgcgacctgc cgtacaaggt gatgttcccc ggcccgacgg gcaccaacgc cgtggaggcc
     4021 gcgctcaagc tggcccgcaa ggtcaagggc cgcgagtcga tcgtctcctt caccaacgcc
     4081 ttccacggca tgtccctcgg gtcgctcgcc gtcaccggca acgccttcaa gcgggccggc
     4141 gccggcatcc cgctggtgca cggcaccccg atgcccttcg acaactacct cgacgggcag
     4201 gtcccggact tcctgtggtt cgaacggctc ctggaggacc agggctcggg cctgaaccag
     4261 cccgccgccg tcatcgtgga gaccgtccag ggcgagggcg gcatcaacgt cgcccgcgcc
     4321 gactggctgc gcgccctcgc cgacctgtgc gagcgccgcg acatgctgct gatcgtcgac
     4381 gacatccaga tgggctgcgg ccgcaccggc gccttcttct ccttcgagga ggcgggcatc
     4441 accccggaca tcgtcaccct gtccaagtcc atcggcggct acgggatgcc gatgtcgctc
     4501 actctgttca agccggagct cgacatctgg gagcccggcg agcacaacgg caccttccgc
     4561 ggcaacaacc cctccttcgt caccgccgcg gccgctctga acgcctactg ggccgacggc
     4621 cagatggaga agcagaccct cgcccggggc gagcaggtcg cgcgggcgct cgacgccatc
     4681 tgcgccgaac accccgggct cggcgggcgg taccggggcc gcggcctcgt ctggggcctg
     4741 gagttcaccg accgcgcccg cgcctcggcc gtcgccgcgc gcgccttcga actcggcctc
     4801 ctcgtcgaga cctccggccc cgcgagcgag gtcgtcaagc tgctccccgc cctgaccatc
     4861 tcccccgagg aactggacga gggcctgcgg accctcgccc gcgcggtccg cgagaccgcc
     4921 tgaccaccgc ccgcccacga cccggccccg caccaccgga aacaccagca ccgacagcac
     4981 cgacagagag aggcaccacc gtgatcgtcc gttcgttcaa ggacatcgag ggcaccgacc
     5041 ggcacgtcaa ggcccggtcc ggcacctggg agagcaagcg catcgtgctc gccaaggagc
     5101 gcgtcggctt ctccctgcac gagaccgtgc tgtacgcggg cacggagacg tcgatgtggt
     5161 acgccaacca catcgaggcc gtgctgtgcg tcgaggggga ggccgagctc accgacgacg
     5221 agaccggcga gaagcactgg atcgagcccg gcaccatgta cctgctggac ggccacgagc
     5281 gccacaccct gcgcccgaag accgacttcc gctgcgtgtg cgtcttcaac ccgccggtca
     5341 ccggccgcga ggaccacgac gagaacggcg tctaccccct gctcacggag gagggctgag
     5401 cgatgacggc cacggtcagc gacctctacc cgacccgggg cacccaggag gtgcccgtac
     5461 cccggcggga cccggtggtg tggtcccgcc ccggcgcccc cggcccgatc ggtgacaccg
     5521 agctggcgga ctacgaccgc gacggcttcc tcacggtcga ccggctcatc ggcccggacg
     5581 aggtggcgcg ctaccgcgcc gagctggacc ggctgatcgc cgacccggcc gtacgcgccg
     5641 acgagcgctc gatcgtcgag ccccggtcgc aggacgtgcg gtcggtgttc gaggtgcacc
     5701 ggatcagcga ggtcttcgcg gagctcgtgc gcgacccgcg cgtggtcggc cgggcccggc
     5761 agatcctcgg ctcggacgtc tacgtccacc agtcccggat caacgtcaag cccgggttcg
     5821 gcgcctccgg cttctactgg cactcggact tcgagacctg gcacgccgag gacgggctgc
     5881 cgcgcatgcg gaccgtgtcg gtctcgatcg ccctcaccga gaacctcgac accaacggcg
     5941 ggctgatgat catgcccggc tcgcaccgga cgttcctcgg ctgcgccggt gagaccccgc
     6001 gcgacaacta caagcggtcg ctgcggatgc aggacgcggg cacgccctcc gacgccgcgc
     6061 tgaccggctt cgccgagcgc cacggcgtcc ggctcttcac cgggccggcc gggtcggcga
     6121 cgtggttcga ctgcaactgc atgcacgggt cagggaacaa catcacgccc ttcccgcgca
     6181 gcaacgtctt catcgtcttc aacagcgtgg acaacgcggc ggtggagccg ttcgcggcac
     6241 cggcgcggcg cccggagttc gtgggggcgc gggacttcac cccggtgccc tgagcgggcc
     6301 cgtcggccgc cgggcgggcc cggcccgccc ggcgaccgac ggctatttac gggcgatgac
     6361 catcgactcg ggccccgcca ggtgctgcac acggctctcc gcgaagcccg ccccggccag
     6421 ccattcgcgg cactcggccc cggtgtagtc gaagccgccg gtggtctcga cgagcatgtt
     6481 gagactcatc agcagcccgg tcagatgggt gcggcggtcg tcgtcgatga gcgtctcgta
     6541 gacgatgacg acgccgcccg ccgggagcgc ctcgtacgcc ttgcgcagca gcatccgctt
     6601 cgtcggcagg tcccagtcgt gcaggatgtg gccgaggagc agggcgtcgg cgcggggcag
     6661 cgggtccgtg aagaagtcgc cggccgcgaa ggtgagccgg tccgcgagcc cgaagcgctc
     6721 ggcgcgccgg tcgaagtcgc cgcgcgcgga ctccaggtcg aagccggtgc cccgcaggtg
     6781 cgggtggcgc agcaggatct ggccgagggt accgccctcg gcgcagccga cgtcggcgac
     6841 gacgcggtgc gcggaccagt cgacggcatc ggcgagggcg tgcgccgagc gcatcgacag
     6901 gcccgtcatg gcctggagga actccgagcg gcggacgggg tcctggtaga gggtcccgaa
     6961 gaagtcaccg ccgtgcttga tctcgttctg cggctcgccg gtgcgcagcc cgtcgaggag
     7021 cgtgccccag aagccgtaga gccggtggtc ggtcatctcc aggaagccgc ccaggtaggt
     7081 ggcgcggttg cggtccaggt agcgggcggt gacgggggtg ttgccgtaac cctcggggcc
     7141 gcgctccagc aggtcgagcg ccaccagcgt gtcgaggaag tcgcgggcgg cgcgggggtg
     7201 caggccgagc cggtcgcgca gttcctcgta cggcagcggg cgcgcggcca gctcggtgaa
     7261 gaggccgagc cggaccgcgc tgaacaggac cttcgagacg gcgaagccga gcccgagctg
     7321 gaggatccgg tcgagggccg cggagtggtc ctgctcgtcg cgcgcacggc gcatgtgctc
     7381 tcctcgggaa gcgggggagg gaaggacgga agaaggcaga gctccgcacg gggctccggc
     7441 ggagcgtccc gtgggcggtg atcttcccgt cctacccact caccggcgtg cgagcacctc
     7501 cagcagccgg tcgacgtcgg cggtggagtt gtagaggtgg aaggcggcgc gaagcctccc
     7561 cgcccgcgcg ctgacctgca cccccgccgc cgccggccgc tcggcggcgc cccggacgcc
     7621 cgggacggcg acgatcgccg agccctccgc cggcacgggc gtgaggccca gcgccaccac
     7681 cccggcgcgg aaccggtcgg cgagcgcccg gtcgtgcgcg ccgatgcgct cgacgcccag
     7741 ctcctccacc agggcgagcc cgtgcagggc ccccacgtac gacaggacgg cgacgttctc
     7801 gtcgaagcgg cgggccgtcg aggcgagcgg ctccaccttg ccgtagcagg tcagccaggg
     7861 gtcctcgccc gtgacccagc ccgcgaacac gggcgtgagg gcgcccaggt cctcggggac
     7921 ggtgaggaag gcgacgccgc gcgggcccat cagccacttg tagcccacac cgacggtgaa
     7981 gtcgtgggcg gcggcgtcca gcgggaacca gcccgccgcc tggctgacgt cgacgagcgt
     8041 acgggcgccg tacgcgcggg cggccgcgga gatcgccccg agggcggcga tccggccgtc
     8101 ggccgactgc acggcgctca ccgcgacgag cgccgtgtcc ggccgcacct cctccgcgag
     8161 ccgctccagc gggaccgtac gcacccggag gccgggccgg gtgtggaagg gattgacgag
     8221 cgaggcgaag tcgccctccg cgacgagcac ttcggcaccg gccggcaggg actgggcgac
     8281 gaggccgcag tacacggcga cggaaccgcc ggcggcgacc cgggcggcgg gcacgccgac
     8341 cagccgggcg taggcggtcc gggccgcgtc caccgtggcc gagaagtcca cgttccccgg
     8401 ggccccggcg gccaggacgc cgatcgcctc gcccatggcg gcggcggccc cggtgggcag
     8461 caggccggtg gccgcggtgt tgagatagac ggtctccggg gcgaaccggc cgggcgcgag
     8521 gctttccatc cacccactat cggcggacgc gatcattccg tccatgcccg gcggagcggg
     8581 ggcgcgcgac cggcccgcgc accccggccg cgggcgcgcc ggcgcggtca gggtccgccc
     8641 ccgacggcgg gatgagggca cacgcgccct gaggaggagc ggtccgggcg cggaccgggc
     8701 cctgcggacc agacgggagc gcgcccggtt ccgccctgcg gccgagggtg attcaccgct
     8761 gcgggggaag cggtggacgg ggtgggcacg gaagccttgg gacatgagcc gaacgcgacg
     8821 gagtccgctc ctcgaagccc ttgccaccac agccgatccg gggttcccga cctgggacga
     8881 ccacgcgacg ttctgcggcg ggaacccgcc cgcgggacgc ccgggagcgc cgcgcgccat
     8941 gcggcgggga gagccgggga ccggccccgg gtagcgggac cgtccggcgc cgtccgcctt
     9001 cgcccgcggc ccggggcagg aggagggcgc ggcgccgggc cggggtgtgc gaacgcccgc
     9061 cgggccgtcc cggggaagga tcccggggcg gcccggcgcc ccgcgcgcgc acaacgggcc
     9121 tcggcgtggc ggcccgccgg gaccgggcgg ccgggcccct ggcgcgggtg gggacgtccg
     9181 cggcccctcc ggaccgccgg cgtcacctac cctgtgcgga tgatcaacgg ttcgcgaaga
     9241 tccgccccgt gacggccctc ctcggccacc gggcccgcct ccgctgggtg cacctcctcc
     9301 tcggcggcgc gctgctgatg ccgtactacc tcctggccga cgtcctcctc gccctggtca
     9361 ccgctcccga cgggggaagc ctcctcctcg ggggctcgct cgcccggcag ttcggcgtct
     9421 tcgcgctggc cctcctcccc gccgcgctca ccgcgctcct gccgctcgta cgccccctgg
     9481 agggcgcggc ggcgcgggcg ctgtgcgggg tgccgggcga ggggctggcc accgggccga
     9541 gcacctcctg ggccgcgcgc ggcaggacgg ccgcctggta cacgctccac ctcacggccg
     9601 gcggcctcgt cagcgggatg tcgctggcca ccccgccggc cgccgtgacg ctgatcgccc
     9661 tgcccttcct cgaccgcgac cgggacgccg ggggcctggg ccgggcggcg gtcttcgccg
     9721 ggccccggct gctcctggcg ccgctcgccg ggctcgccct gctggccctg atcgtggtct
     9781 cctcctgggc ggcgggcgcg ctcctggcgc gctgcgcgcc cgtcctcctg ggacccacgg
     9841 cggccgaccg gctggccgcc gccgagcggc acgcgaccca tctcgcgctc cgcaaccggc
     9901 tggcccgcga gctgcacgac tccgtgggcc acgcgctgag cgccgtcacc ctccaggcga
     9961 gcgccgcgcg cagggtcctc gactccgacc ccgagttcgc ccggcaggcg ctcgcggcca
    10021 tcgaggcgac gacgcgggac gcggtcggcg aactcgacac ggtcctcggc ctcttgcgcg
    10081 aggacggcac ggcctccgcc gcgcccgcgc cgacgctcgc cgccctggac gccctcctgg
    10141 agcgcacccg cgcggcgggc ggcacggtcg tgcccaccgt cgacgccgcc ctggaccgtg
    10201 tgccggcggt cgtctcccgc gaggcctacc gcatcgtcca ggagggcctc accaacgcct
    10261 tgcggcacgc ggggccggtg ccggtgcggc tcggcatcgc ctcgcgcggg ggcgtcctgg
    10321 aagtgacctt ggagaacccg gtggcggggg tggtgccggg ggcccggcgg acgggtggtg
    10381 ggcgggggct ggcggggat
//
