LOCUS       NZ_KZ195574            10399 bp    DNA     linear   CON 06-SEP-2024
DEFINITION  Streptomyces sp. CS113 scaffold00001, whole genome shotgun sequence.
ACCESSION   NZ_KZ195574
VERSION     NZ_KZ195574.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. CS113
  ORGANISM  Streptomyces sp. CS113
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8695358)
  AUTHORS   Malmierca,M.G., Gonzalez-Montes,L., Perez-Victoria,I., Sialer,C.,
            Brana,A.F., Garcia Salcedo,R., Martin,J., Reyes,F., Mendez,C.,
            Olano,C. and Salas,J.A.
  TITLE     Searching for Glycosylated Natural Products in Actinomycetes and
            Identification of Novel Macrolactams and Angucyclines
  JOURNAL   Front Microbiol 9, 39 (2018)
   PUBMED   29441046
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8695358)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Searching for glycosylated natural products in actinomycetes
            isolated from leaf-cutting ants: activation of two silent clusters
            and identification of a novel macrolactam
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8695358)
  AUTHORS   Malmierca,M.G., Mendez,C., Olano,C. and Salas,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAY-2017) Biologia Funcional, Area de Microbiologia,
            Universidad de Oviedo, Julian Claveria S/N, Oviedo, Asturias 33006,
            Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 78.17x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002188365.1-RS_2024_09_06
            Annotation Date                   :: 09/06/2024 02:31:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,790
            CDSs (total)                      :: 7,719
            Genes (coding)                    :: 7,614
            CDSs (with protein)               :: 7,614
            Genes (RNA)                       :: 71
            rRNAs                             :: 2, 1 (5S, 16S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 105
            CDSs (without protein)            :: 105
            Pseudo Genes (ambiguous residues) :: 0 of 105
            Pseudo Genes (frameshifted)       :: 35 of 105
            Pseudo Genes (incomplete)         :: 81 of 105
            Pseudo Genes (internal stop)      :: 4 of 105
            Pseudo Genes (multiple problems)  :: 15 of 105
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:56:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6631529
            Orig. end                         :: 6641928
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KZ195574.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Prof. Salas's laboratory, A.
            Microbiology, University of Oviedo, Spain.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="25"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    618..633
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.08"
                     /tool="antismash"
     gene            1404..2012
                     /locus_tag="B9W62_RS29760"
                     /old_locus_tag="B9W62_29780"
     CDS             1404..2012
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="B9W62_RS29760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_29780"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_087810040.1"
                     /transl_table=11
                     /translation="MTLQLPELPFALRPYGPDGRWSYADGVLTGVAGARQDRFVPPTGE
                     SLESVSDAPRLLGAPDGDFQLIARVSVGFRGAFDAGVLYVHVGERAWAKLCLEYSPDVP
                     TVCTVVTRGHSDDCNSFTVDGDSVWFRVSRTGRAFAFHASLDGKRWTFVRLFTLGDEEE
                     TGAALVGFLAQAPTGEGCTVTYDHLEFRPAWPADLRDGS"
     gene            2104..2817
                     /locus_tag="B9W62_RS29765"
                     /old_locus_tag="B9W62_29785"
     CDS             2104..2817
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976951.1"
                     /locus_tag="B9W62_RS29765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_29785"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_087810041.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALAAFPHRDGVEVVHRSFELDP
                     GRAKDDVQPVLTMLTAKYGMSQEQAQAGEDNLGAQAAAEGLDYRTRDRDHGSTFDMHRL
                     LHLAKERGRHDALLDAFYRGNFADERSVFNDDARLVELAAGAGLDAEEVRAVLADPDAY
                     AEAVRADEREAAQLGATGVPFFVLDRAYGVSGAQPAEVFTQALTQAWGERTPLKLIDDG
                     GAEACGPDGCAVPGN"
     gene            2877..3971
                     /locus_tag="B9W62_RS29770"
                     /old_locus_tag="B9W62_29790"
     CDS             2877..3971
                     /NRPS_PKS="Domain: Aminotran_5 (28-319). E-value: 1.2e-22.
                     Score: 72.2. Matches aSDomain:
                     nrpspksdomains_B9W62_RS29770_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448151.1"
                     /locus_tag="B9W62_RS29770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_29790"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_087810042.1"
                     /sec_met_domain="Aminotran_5 (E-value: 5.2e-17, bitscore:
                     60.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDGEVTGPHSGAMESFENLVPTEFAPKTTYLNTASTGLLPARTLV
                     AMRDAAASAAAGQPLDMFADVEASRAAYARLVGVPATRVAAGASVATFSGLVAASLPPG
                     AEVLTAEADFSSLVNPLYTRGDLKVRSVPLERLAESVGPDTALVAVSSVQSADGRLADL
                     AALRDAARAHGARTLIDASQSNGWLPLAAGDFDFVAAVAYKWVLCPRGVAFLVVPEDLG
                     AAAGLTPLFAGWASGEHPWDSCYGPVEELAHSARRFDESPALFSYTGARHSLALIEELG
                     VDAVHAHDTGLADRFRAGVRSLGHEPVAAPGSAIVSVPGLGHRQGELGGAGVQVSDRAG
                     NLRAAFHVYNTAVDVDRLLDVLAG"
     aSDomain        2961..3833
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9W62_RS29770_Aminotran_5.1"
                     /evalue="1.20E-22"
                     /label="B9W62_RS29770_Aminotran_5.1"
                     /locus_tag="B9W62_RS29770"
                     /protein_end="319"
                     /protein_start="28"
                     /score="72.2"
                     /tool="antismash"
                     /translation="TYLNTASTGLLPARTLVAMRDAAASAAAGQPLDMFADVEASRAAY
                     ARLVGVPATRVAAGASVATFSGLVAASLPPGAEVLTAEADFSSLVNPLYTRGDLKVRSV
                     PLERLAESVGPDTALVAVSSVQSADGRLADLAALRDAARAHGARTLIDASQSNGWLPLA
                     AGDFDFVAAVAYKWVLCPRGVAFLVVPEDLGAAAGLTPLFAGWASGEHPWDSCYGPVEE
                     LAHSARRFDESPALFSYTGARHSLALIEELGVDAVHAHDTGLADRFRAGVRSLGHEPVA
                     APGSAIVSVP"
     gene            complement(4098..4994)
                     /gene="thpD"
                     /locus_tag="B9W62_RS29775"
                     /old_locus_tag="B9W62_29795"
     CDS             complement(4098..4994)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="B9W62_RS29775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_29795"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_179235734.1"
                     /sec_met_domain="PhyH (E-value: 8.1e-42, bitscore: 142.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTNVTDLYPTRGATEVATPRQDPVVWGSPDTPGPVPAGDLQS
                     LERDGFLAIDQLIAPDEVAEYQRELERLTTDPAIRADERSIVEPQSKEIRSVFEVHKIS
                     EVFAKLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPN
                     MRTISVSIALTENYDTNGGLMIMPGSHKTFLGCAGATPKDNYKKSLQMQDAGTPSDEAL
                     TKMASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENTAVEPFAA
                     PIRRPDFIGARDFTPVK"
     misc_feature    complement(4985..5005)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.15"
                     /tool="antismash"
     gene            complement(5001..5399)
                     /locus_tag="B9W62_RS29780"
                     /old_locus_tag="B9W62_29800"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037840.1"
                     /locus_tag="B9W62_RS29780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_29800"
                     /product="ectoine synthase"
                     /protein_id="WP_030184764.1"
                     /sec_met_domain="ectoine_synt (E-value: 6.1e-54, bitscore:
                     180.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKEFEGTDRHVKSASGTWESTRIVLAKEKVGFSLHETILY
                     AGTETSMWYANHIEAVVCTKGDAELTDRETGKTYHITPGTMYLLNGHERHTLKVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEEV"
     gene            complement(5529..6800)
                     /gene="ectB"
                     /locus_tag="B9W62_RS29785"
                     /old_locus_tag="B9W62_29805"
     CDS             complement(5529..6800)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-366). E-value: 2.4e-72.
                     Score: 235.7. Matches aSDomain:
                     nrpspksdomains_B9W62_RS29785_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="B9W62_RS29785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_29805"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_087810043.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.1e-76, bitscore:
                     256.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTIFDRAVGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNAVLKRALIDYLERDGVTHGLDMSTTAKRRFLETFQNTILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVEDFIWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALAALCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMA
                     LTLFKPELDVWEPGEHNGTFRGNNPAFVTATATLEAYWADGSAMEKQTRKRGEQVEQHM
                     IAITEENLADVKEYRGRGLVWGLEFHDKGRANRVAKRAFELGLLIETSGPESEVVKLLP
                     ALTITPDELDEGMKTLARAVRETA"
     aSDomain        complement(5703..6713)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9W62_RS29785_Aminotran_3.1"
                     /evalue="2.40E-72"
                     /label="B9W62_RS29785_Aminotran_3.1"
                     /locus_tag="B9W62_RS29785"
                     /protein_end="366"
                     /protein_start="29"
                     /score="235.7"
                     /tool="antismash"
                     /translation="DRAVGSRMYDEDGHEYLDFFAGAGSLNYGHNNAVLKRALIDYLER
                     DGVTHGLDMSTTAKRRFLETFQNTILRPRDLPYKVMFPGPTGTNAVESALKLARKVKGR
                     ESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVEDFIWFERL
                     LEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALAALCERQDMLLIVDDIQMGCGRT
                     GAFFSFEEAGITPDIVTVSKSISGYGLPMALTLFKPELDVWEPGEHNGTFRGNNPAFVT
                     ATATLEAYWADGSAMEKQTRKRGEQVEQHMIAITEENLADVKEYRGRGLVWGLEFH"
     gene            complement(6926..7438)
                     /gene="ectA"
                     /locus_tag="B9W62_RS29790"
                     /old_locus_tag="B9W62_29810"
     CDS             complement(6926..7438)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448158.1"
                     /locus_tag="B9W62_RS29790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_29810"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_087810044.1"
                     /transl_table=11
                     /translation="MTAAQADLQIDRPRVADGAALWRIARDSKVLDLNSSYSYLLWCRD
                     FAATSAVVRDGHGVPVGFVTGYVRPDSPDTLLVWQVAVDRTYRGRGLAATLLDGLAARV
                     VHERGITVLETTISPDNTASQRLFTSFAERRGARLEREVLFDTAVFPDGPHEPEVLYRI
                     GPLSATG"
     misc_feature    complement(7437..7449)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(7463..7475)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(7594..7609)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7604..7619
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     gene            complement(7768..8895)
                     /locus_tag="B9W62_RS29795"
                     /old_locus_tag="B9W62_29815"
     CDS             complement(7768..8895)
                     /NRPS_PKS="Domain: Aminotran_5 (73-291). E-value: 8.9e-29.
                     Score: 92.4. Matches aSDomain:
                     nrpspksdomains_B9W62_RS29795_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976957.1"
                     /locus_tag="B9W62_RS29795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_29815"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_087810045.1"
                     /sec_met_domain="Aminotran_5 (E-value: 8.3e-22, bitscore:
                     76.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLSAARFAAIEDRVARLLGTGQDVVVMQGEALLPLE
                     GAIRAAAGPGTVALNIVTGPYGQTFGNWLRDCGATVHDLAVPFHTAVSAEQVRRALAEH
                     PETDFVSLVHAEAATGNTNPVAEVGEVVRAHGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPNAPRRSYLSLLDWKERWIDGGRKALLHAP
                     AQLEMLALEACVERIEAEDPETVMAQHASAAAATRAGALALGGVEPYVYEARDAAPVAT
                     TLRTPSDVDASALVARALESDPALPLAAGGGALAGEMIRVNHYGPDATRGVVLSCLTAL
                     GAALAERGVKADPDAARRAVEETWGRPTGPGLSEG"
     aSDomain        complement(8023..8676)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B9W62_RS29795_Aminotran_5.1"
                     /evalue="8.90E-29"
                     /label="B9W62_RS29795_Aminotran_5.1"
                     /locus_tag="B9W62_RS29795"
                     /protein_end="291"
                     /protein_start="73"
                     /score="92.4"
                     /tool="antismash"
                     /translation="LRDCGATVHDLAVPFHTAVSAEQVRRALAEHPETDFVSLVHAEAA
                     TGNTNPVAEVGEVVRAHGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVS
                     AVSVSERAWARMAANPNAPRRSYLSLLDWKERWIDGGRKALLHAPAQLEMLALEACVER
                     IEAEDPETVMAQHASAAAATRAGALALGGVEPYVYEARDAAPVATTLRTPSDVDA"
     gene            9101..9583
                     /locus_tag="B9W62_RS29800"
                     /old_locus_tag="B9W62_29820"
     CDS             9101..9583
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028017.1"
                     /locus_tag="B9W62_RS29800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B9W62_29820"
                     /product="hypothetical protein"
                     /protein_id="WP_087810046.1"
                     /transl_table=11
                     /translation="MFLVLLLVVVVAVVTAVGLTGHGASVLAARRARGPGAGPAPRALA
                     AFAGAAAVAVYAWGLLHVTGAVMESNGGAGSAPAPPCRTPGNEERALHVIDQSVSFLPL
                     GFVCETTDGGAYTSDDVPGYVNPGAAALALTAVASAVAAGRAAGPRTRAAARDDRR"
ORIGIN
        1 ctaccagctg cgggccttcg ccgacatcgt cgacgagggc gagttcctcg gggtggtcgt
       61 caaccacccc gacgacaccg ccaccccgcc catccccgac ctgcgccgct acaaggtgcc
      121 gctcggcgtg gtcgccgtct actcggcctc caacttcccc ttcgccttct ccgtccccgg
      181 tggcgacacc gccagcgccc tcgcggccgg ctgcccggtc gtcgtcaagg cgcaccccga
      241 ccacccggcc ctgtccgagc tggtcgccaa ggtgctgcgc cgggccgcgg cccgccacgg
      301 catcccggac ggggtcctcg gcctggtgca cggcttcgag gcgggcgtcg agctgatcaa
      361 gcacccgctg gtcgccgcgg cgggcttcac cgggtcgatc cgcggtggcc gcgcgctgtt
      421 cgacgcggcg gccgcgcgtc cggtgccgat cccgttccac ggcgaactgg gctcgctgaa
      481 cccggtcgtc gtcactcagg cggcggccgt ggagcgggcg gaggcgatcg gcgcgggcct
      541 ggccggttcg atgacgctgg gcgtcggcca gttctgcgtc aagcccggtc tggtcctcgt
      601 accggccggc gacgccggtg acggcctggt caagtcgctc accgacgcgg tcagcgccac
      661 cgacgccggt gtgctcctcg accaccgcat gcgggacaac ttcgtcgcgg gcgtcgccga
      721 gcgcgccgcg ctgcccgacg tggactcccc ggtgacgccc ggctcgggcg gcgagcacac
      781 cgtcagcccc gggttcctca cggtgccggc ggcgcggctg gcggcggagg gcgagcacga
      841 cctgctcctg gaggagtgct tcgggccggt caccgtggtg gcccgctacg aggacgaggg
      901 ggaggtgcgg ggggtgctgt cgcggctgcc ggggaacctc acggcgacgg tgcagctctc
      961 tgccgaggaa gtggcgggag agggcgccgg ggcggctctc ctcgcggagg tgacgccgtt
     1021 ggcggggcgg gtcctggtca acggctggcc cacgggggtt gccgtggcgg cggctcagca
     1081 gcacgggggg ccgtacccgg cgaccacgtc gacgtccacg tcggtggggg gtacggccat
     1141 cgagcggtgg ctgcggccgg tggcgtatca gggggtgccg gaggcgctgc ttcctgtgga
     1201 gctgcgggat gacaatccgc tggggctgcc gaggcggttc aacggggttc tcgagcggta
     1261 gggattcgcg ggtgcgccgt aggggtgcgg ttgttcggcg ggtgctgcgc cgtcgtggcc
     1321 ggtcgcgccc acgcggcgga gccgcacatc gatacagtcc cgcgcccctt aaaagcgttc
     1381 actgagcctg cgatgatcag tgcatgactc ttcagcttcc cgaactgccg ttcgccctcc
     1441 gcccgtacgg gcccgacggt cgttggtcct acgccgacgg tgtgctcacc ggggtcgccg
     1501 gggcccggca ggaccggttc gtgccgccca ccggggagtc gctggagtcc gtgtccgacg
     1561 cgccgcggtt gctcggggcg ccggacgggg acttccagct gatcgcgcgg gtgtccgtcg
     1621 ggttccgggg ggccttcgac gccggggtgc tctacgtgca cgtgggggag cgggcctggg
     1681 ccaagctgtg tctggagtac tcgcccgacg tgcccaccgt ctgcacggtg gtcacccgcg
     1741 ggcactccga cgactgcaac tccttcaccg tggacgggga ctccgtctgg ttccgggtca
     1801 gccggaccgg acgggccttc gccttccacg cctcgctcga cgggaagcgc tggaccttcg
     1861 tacggctctt caccctggga gacgaggagg agaccggcgc ggccctcgtc ggcttcctgg
     1921 cgcaggcgcc gacgggggag ggctgcaccg tgacgtacga ccacctggag ttccggccgg
     1981 cctggccggc ggacctgcgg gacggcagct gagcgcgggc gggaatgaga tccgctgctt
     2041 gaacgttcac gtacggggcg gagagggcct ccgccgccgt acgacctgga gaagagccga
     2101 gacatgcgcg tcgagatctg gagcgacatc gcctgcccct ggtgctacgt gggcaaggcc
     2161 cgcttcgaga aggcgctcgc ggccttcccg caccgggacg gggtcgaggt ggtccaccgc
     2221 tccttcgagc tggaccccgg acgggccaag gacgacgtac agccggtgct gacgatgctc
     2281 accgccaagt acggcatgag ccaggagcag gcgcaggccg gtgaggacaa cctcggcgcg
     2341 caggccgccg ccgagggcct ggactaccgc acccgggacc gcgaccacgg cagcaccttc
     2401 gacatgcacc gcctgctgca cctggccaag gagcgggggc ggcacgatgc cctgctggac
     2461 gcgttctacc ggggcaactt cgccgacgag cgctccgtgt tcaacgacga cgcgcggctc
     2521 gtggagctgg ccgccggtgc cgggctcgac gccgaggagg tccgtgcggt gctcgccgac
     2581 cccgacgcct acgccgaggc ggtgcgcgcc gacgagcggg aggccgccca gctcggggcc
     2641 accggggtgc cgttcttcgt gctcgaccgc gcctacggcg tctcgggcgc ccagcccgcc
     2701 gaggtcttca cccaggcact gacccaggcc tggggcgagc gcacgccgct gaagctgatc
     2761 gacgacggcg gcgccgaggc ctgcggcccg gacggatgcg cggtccccgg caactgaagc
     2821 ggcaggccgg ggcggacgca taagcggccc ttgggggtac cgcgtagaaa tacgcgatgg
     2881 acggggaggt cacggggccg cacagtggtg ccatggagag cttcgagaac ctcgtcccta
     2941 ccgagttcgc cccgaagacc acgtacctga acaccgccag caccggcctg ctgcccgccc
     3001 ggaccctcgt ggccatgcgc gacgcggcgg cctccgccgc ggcggggcag ccactcgaca
     3061 tgttcgccga cgtcgaggcg tcccgggccg cgtacgcccg tctcgtggga gtgcccgcca
     3121 cccgggtcgc ggccggcgcg tccgtcgcca cgttcagcgg gctcgtcgcg gcgtcgctgc
     3181 ccccgggcgc cgaagtcctc accgcggagg ccgacttcag ctccctggtg aacccgctgt
     3241 acacgcgcgg cgacctgaag gtgcgcagcg tgccgctgga acggctggcc gagtcggtgg
     3301 gtcccgacac cgcgctcgtc gcggtcagtt ccgtgcagtc cgccgacggg cggctcgccg
     3361 acctcgccgc gctgcgggac gccgcccggg cgcacggggc gcggacgctg atcgacgcct
     3421 cgcagtcgaa cgggtggctg ccgctggcgg ccggcgactt cgacttcgtg gccgccgtcg
     3481 cctacaagtg ggtgctgtgc ccgcgcggcg tcgccttcct cgtcgtaccg gaggacctcg
     3541 gggcggcggc cgggctgaca ccgctgttcg ccgggtgggc ctccggcgag cacccctggg
     3601 acagctgcta cggccccgtc gaggaactcg cgcactccgc ccggcggttc gacgagagcc
     3661 ccgccctgtt ctcctacacc ggcgcccggc actccctggc cctgatcgag gaactgggcg
     3721 tcgacgcggt gcacgcccac gacaccgggc tcgccgaccg gttccgggcg ggggtgcgct
     3781 cgctgggcca cgagccggtg gccgcgccgg gctccgcgat cgtgtccgtg ccgggactcg
     3841 gacaccggca gggcgagctg ggcggggcgg gcgtccaggt ctcggaccgg gcgggcaacc
     3901 tgcgggccgc cttccacgtg tacaacaccg cggtcgacgt ggaccggctg ctcgacgtgc
     3961 tggcgggctg acccggcagg tccggcgggt ccggcaggtc cggcgagtcc ggcagctccg
     4021 gccggtccgg gaagaaatat cgggcagggg cctcccgtcc cacacgagaa ggcccctgcc
     4081 cgagtctcag acgcgggtca cttcaccggg gtgaagtccc gcgcgccgat gaagtccggc
     4141 cgtcggatcg gcgccgcgaa cggctccacg gccgtgttct ccacactgtt gaacacgatg
     4201 aacacgttgc tgcgcgggaa cggcgtgatg ttgtcgcccg acccgtgcat gcagttgcag
     4261 tcgaaccagg tcgccgagcc ggccttgccg gtgaacagct tgatgccgta ctcggaggcc
     4321 atcttggtca gcgcctcgtc ggacggcgtc cccgcgtcct gcatttgcag ggacttcttg
     4381 tagttgtcct tcggcgtggc ccccgcgcac ccgaggaacg tcttgtgcga ccccggcatg
     4441 atcatcagac cgccgttggt gtcgtagttc tcggtgagcg cgatcgagac cgagatcgtg
     4501 cgcatgttgg gcaggccgtc ctcggcgtgc caggtctcga agtccgagtg ccagtagaag
     4561 ccgctggccc cgaagcccgg cttgacgttg atccgcgact ggtggacgta gacgtccgag
     4621 ccgaggatct gccgcgcccg cccgaccacg cgctcgtcgc gcaccagctt ggcgaagacc
     4681 tcactgatct tgtgcacctc gaagaccgac cggatctcct tggactgcgg ctccacgatc
     4741 gagcgctcgt ccgcgcggat ggccgggtcc gtggtgagcc gctccagctc ccgctggtac
     4801 tcggcgacct cgtccggcgc gatcagctgg tcgatggcga ggaagccgtc gcgctccagc
     4861 gactggaggt cacccgccgg gaccgggccg ggcgtgtccg gggagcccca gaccaccggg
     4921 tcctgccggg gagtcgccac ctcggtggcg ccgcgggtgg gatagagatc ggtgacgttc
     4981 gtggtcgtgg tcacggtgac tcacacctcc tcggtgagca gcgggtagac gccgttctcg
     5041 tcgtgatcct cccgtccggt gacgggcggg ttgaagacac agatgcagtg gaagtcctcc
     5101 ttgaccttga gcgtgtgccg ctcgtggccg ttgaggaggt acatcgtgcc cggcgtgatg
     5161 tggtacgtct tccccgtctc gcggtcggtc aactcggcgt cgcccttggt gcagacgacg
     5221 gcctcgatgt ggttggcgta ccacatcgac gtctccgtac ccgcgtacag gatcgtctcg
     5281 tgcagggaga agccgacctt ctccttggcg aggacgatcc gggtgctttc ccaggtgccg
     5341 gacgcgctct tcacgtgccg gtcggtgcct tcgaactcct tgaacgaacg gacgatcacg
     5401 gtgttgcgat gcctcctagg tgaagggtgt gcaaggggcc ccggcgtctt cggcctcgcg
     5461 ggccgaagac gccgggagtc atggggtggg tgcccgacgg ccaagccgtc agacggtcac
     5521 gaggacggtc acgcggtctc gcgcacggcc cgggccaggg tcttcatgcc ctcgtccagc
     5581 tcgtcgggcg tgatggtgag cgcgggcagc agcttgacga cctcgctctc ggggccggag
     5641 gtctcgatga gcagccccag ctcgaaggcg cgcttcgcga cccggttcgc gcggcccttg
     5701 tcgtggaact ccaggcccca gaccaggccg cggccgcggt actccttgac gtcggcgagg
     5761 ttctcctcgg tgatggcgat catgtgctgc tcgacctgct cgccgcgctt gcgggtctgc
     5821 ttctccatcg ccgacccgtc ggcccagtag gcctccagcg tggcggtcgc cgtgacgaag
     5881 gccgggttgt tgccgcggaa ggtgccgttg tgctcgcccg gctcccacac gtccagctcc
     5941 ggcttgaaca gggtcagcgc catcggcagg ccgtagccgc tgatcgactt ggagacggtg
     6001 acgatgtccg gcgtgatgcc cgcctcctcg aaggagaaga aggcgccggt gcggccgcag
     6061 cccatctgga tgtcgtcgac gatgagcagc atgtcctggc gttcgcacag ggcggccagg
     6121 gcgcgcagcc actcggcgcg ggcgacgttg atgccgccct cgccctgcac cgtctcgacg
     6181 atcacggcgg ccggcttgtt caggccggag ccctggtcct cgaggagccg ctcgaaccag
     6241 atgaagtcct cgacggtgcc gtcgaagtag ttgtcgaacg gcatgggcgt gccgtgcacc
     6301 agcgggatgc cggcaccggc ccgcttgaag gcgttgccgg tcaccgcgag ggagccgagc
     6361 gacatgccgt ggaaggcgtt ggtgaacgac acgatcgact cgcgcccctt caccttgcgc
     6421 gccagcttca gcgcggactc gacggcgttg gtgcccgtcg ggcccgggaa catgaccttg
     6481 tacggcaggt cgcgggggcg caggatcgtg ttctggaacg tctccaggaa gcggcgcttg
     6541 gcggtggtcg acatgtcgag cccgtgcgtg acgccgtccc gctccaggta gtcgatcagc
     6601 gcgcgtttca gcacggcgtt gttgtgcccg tagttgagcg aacccgctcc ggcgaagaag
     6661 tcgaggtact cgtggccgtc ctcgtcgtac atgcggctgc cgaccgcacg gtcgaagatg
     6721 gtgggccagc cgcggcagta gctgcgcacc tcggactcga cggtctcgaa gacgctgagg
     6781 tcgggctggg tgatggtcac gacgaatcgc tcctcgtggg gtgaggtggg agaggaaggt
     6841 cgtacggatc cggggaccgg tgcggtccgc cgggaagtct gggggccgga ctggcgcggg
     6901 gtgccgggcc gtccggacgg ccgggtcagc cggtggcgga cagggggcct atgcggtaca
     6961 gcacctcggg ctcgtgcggc ccgtcgggga acaccgccgt gtcgaacagg acttcgcgct
     7021 caagccgggc gccgcggcgc tcggcgaacg aggtgaacag tcgttgggac gccgtgttgt
     7081 cgggggagat cgtcgtctcc aagacggtga tcccgcgttc gtggaccacc cgggccgcca
     7141 gtccgtccag gagcgtggcc gcgagcccac gaccacggta cgtccggtcc accgccacct
     7201 gccacacgag cagggtgtcg gggctgtcgg gccgtacgta gccggtgacg aagccgaccg
     7261 gcacgccgtg cccgtcgcgc acgacggccg acgtggcggc gaagtcccgg caccagagca
     7321 gatagctgta ggaggagttg aggtcgagga ccttggaatc cctcgctatc cgccacaggg
     7381 cggcaccgtc ggccactctc ggacgatcga tttgcaggtc tgcttgcgcg gcagtcatgc
     7441 aaattgaatt tacccagcga aattcgaaat tgcatcgccg gatggggtta cgaatgggcg
     7501 ccccgtgtgc tatcacgcgg gcgcgtgcca ccgcgcgaga cggcggcgtt atgtgggggt
     7561 ttgacgggga tgtaagccgc aaagcgggca gcatgtggat cgggtcacaa tcgcgtaacc
     7621 cgtgcgagac gtgtccgaaa tgtgaagatc ggtagtcgtt aaatctttgt gtttaggtcc
     7681 ggggaaagcg ggcagaagaa tacgggaagc tgcgctgagt gaaattcatg aattctgctt
     7741 gtgcgagcat ccggaattcc ctccgattca tccttccgaa aggccgggtc cggtcggccg
     7801 cccccacgtc tcctcgaccg cccgccgggc ggcgtccggg tcggccttca cgccccgctc
     7861 ggccagcgcg gcacccagtg ccgtcaggca ggacagcacg accccgcgcg tcgcgtccgg
     7921 gccgtagtgg ttgacccgga tcatctcgcc cgccagcgcg ccgccgcccg cggccagcgg
     7981 cagcgcggga tcggactcca gggcacgggc caccagcgcg gacgcgtcga cgtccgacgg
     8041 cgtgcgcagc gtcgtcgcga ccggcgccgc gtcccgcgcc tcgtacacgt acggctcgac
     8101 gccgcccagc gcgagcgcgc cggcccgggt ggccgccgcg gcggacgcgt gctgcgccat
     8161 caccgtctcg ggatcctccg cctcgatgcg ctcgacacag gcctccagcg cgagcatctc
     8221 caactgcgcc ggcgcgtgca gcagcgcctt gcgcccgccg tcgatccagc gctccttcca
     8281 gtccaggagc gacaggtacg accgccgcgg ggcgttcggg ttcgccgcca tccgcgccca
     8341 ggcccgctcg ctcaccgaca ccgccgagac cccggccggg ccgcccatcg ccttctgcgc
     8401 gccgatcacg cacaggtcca cgccccacgc gtccggcagc accggctcgg cgcccacgga
     8461 ggccaccgcg tccaggtaga acagcgcccc gtgcgcccgc accacctcgc cgacctccgc
     8521 gaccgggttg gtgttgccgg tcgccgcctc ggcgtgcacg agggacacga agtcggtctc
     8581 cgggtgctcg gcgagggccc ggcggacctg ctcggcggag accgccgtgt ggaacggcac
     8641 ggcgaggtcg tgcacggtcg cgccgcagtc gcgcagccag ttcccgaagg tctgcccgta
     8701 gggccccgtg acgatgttca gcgcgacggt gccgggaccc gcggcggcgc ggatcgcgcc
     8761 ctccaggggc agcagcgcct cgccctgcat gacgaccacg tcctgtccgg tgccgagcag
     8821 gcgcgcgacc cggtcctcga tcgcggcgaa acgtgcggcg ctcagcgggg ggaggtccag
     8881 gaaggggtgg gtcacggcgg tgctctcttc ggtaacggcg ggtgtgcggc gggtacgccg
     8941 ggtacggacg cgcgtacgtc gcgtacgtcg cgtacggagt cgagagtaac catggcgccc
     9001 gccggcactc gcgccggagg gcttccgttg cgacaccccc acgcccggaa tcgtggcttc
     9061 cgtcacagcc ggagaggtcg ccgcctgatg gactggcccc gtgttcctgg tactcctgct
     9121 ggtcgtcgtg gtcgccgtgg tgacggccgt cggtctgacc gggcacggcg cgagcgtcct
     9181 cgccgcccgc cgtgcccggg gtccgggcgc cggacccgca ccgcgcgccc tggccgcctt
     9241 cgccggtgcc gccgccgtcg cggtgtacgc ctgggggctg ctgcacgtga ccggcgccgt
     9301 catggagtcg aacggcggcg ccggctccgc acccgcgccg ccctgccgca cccccgggaa
     9361 cgaggaacgc gccctccacg tcatcgacca gtcggtctcc ttcctgcccc tgggcttcgt
     9421 ctgcgagacc accgacggcg gcgcgtacac cagcgacgac gtccccggct acgtcaaccc
     9481 cggcgccgcg gccctggccc tgaccgccgt cgcgagcgcc gtcgcggccg ggcgcgcggc
     9541 cgggccgcgc acccgcgcgg cagcgcgcga cgaccgccgg tgaccggccc cggacggggg
     9601 tgcgggcacc gtttagggtg cggggcatga gcgatcgcgc ggtgctgcac gtgaagggtc
     9661 gggtgctcgt cggaccggag gaggtccggg acgaggtgtg ggtgatcgac ggccggatct
     9721 cgtacgaccg ccccaccggc gcggccgacg tccgcaccgt cgagggctgg gcgctgcccg
     9781 gcctggtcga cgcgcactgc cacgtcggcc tggaccagca cggcgcggtc cccgaggacg
     9841 tcgccgagaa gcaggcgctg accgaccgcg aggcgggcac cctcctgatc cgcgacgccg
     9901 gctcgccctc cgacacccgc tggatcgacg accgcgagga cctcccgaag atcatccggg
     9961 ccgggcggca catcgcccgc acccgccgct acatccgcaa ctacgcctgg gaggtcgaac
    10021 ccgaggacct cgtcgcgtac gtcgcccagg aagcccggcg cggcgacggc tgggtgaagc
    10081 tggtcggcga ctggatcgac cgcgacctgg gcgacctgtc ggcctgctgg ccgcgcggcg
    10141 cggtcgaggc ggccatcgcg gaggcccacc gcctcggcgc ccgcgtcacg gcgcactgct
    10201 tcgccgagga ctccctgcgc gacctggtcg aggccgggat cgactgcgtc gagcacgcca
    10261 cggggctcac ggacgacacg atccccctgt tcgccgaacg cggcgtcgcc atcgtgccca
    10321 cgctggtcaa catcgccacc ttccccgcgc tggccgacgg cggagagcgg cgcttcccgc
    10381 gctggtcggc ccatatgcg
//
